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System for evaluating a company's customer equity

The object of the present invention is to provide a system for evaluating the customer equity of products and services provided by a company, with consideration of customer-purchase trends, and to provide a means for evaluating the ratio of repeat customers and the customer-equity growth ratio by store, region, and / or customer group, and to provide data for establishing an optimal plan for increasing sales. A system 100 for evaluating customer equity according to the present invention comprises a computer system 2 for conducting sales management for a company, and an evaluation computer 10 connected to the computer system 2 via a communications line 5. The evaluation computer 10 comprises a Web server 11, a communications controller 15, an application server 12, and a database 14 for recording purchase records received from a company or store. The database comprises a table of original records 14a for recording purchase records in the order that they are generated, a master table of customers 14b, and a tabulation table 14c for sorting records by time period. The application server 12 comprises a purchase-data collecting means 12a, a customer-equity sorting means 12b, and a customer-equity evaluation means 12c. The customer-equity evaluation means 12c comprises a total-customer-equity tabulating means 12c-1; an average-customer-equity tabulating means 12c-2; a means of tabulating the customer-stability ratio 12c-3; and a customer-equity growth-ratio tabulating means 12c-4.
Owner:DENTSU TEC INC

Method and system for rapid biomolecular recognition of amino acids and protein sequencing

Methods, compositions, kits, and apparatus are provided wherein the aminoacyl-tRNA synthetase system is used to analyze amino acids. The method allows very small devices for quantitative or semi-quantitative analysis of the amino acids in samples or in sequential or complete proteolytic digestions. The methods can be readily applied to the detection and/or quantitation of one or more primary amino acids by using cognate aminoacyl-tRNA synthetase and cognate tRNA. The basis of the method is that each of the 20 synthetases and/or a tRNA specific for a different amino acid is separated spatially or differentially labeled. The reactions catalyzed by all 20 synthetases may be monitored simultaneously, or nearly simultaneously, or in parallel. Each separately positioned synthetase or tRNA will signal its cognate amino acid. The synthetase reactions can be monitored using continuous spectroscopic assays. Alternatively, since elongation factor Tu:GTP (EF-Tu:GTP) specifically binds all AA-tRNAs, the aminoacylation reactions catalyzed by the synthetases can be monitored using ligand assays. Microarrays and microsensors for amino acid analysis are provided. Additionally, amino acid analysis devices are integrated with protease digestions to produce miniaturized enzymatic sequenators capable of generating either N- or C-terminal sequence and composition data for a protein or peptide. The possibility of parallel processing of many samples in an automated manner is discussed.
Owner:NANOBIODYNAMICS

Lens and associatable flow cell

PCT No. PCT/US97/04398 Sec. 371 Date Sep. 18, 1998 Sec. 102(e) Date Sep. 18, 1998 PCT Filed Mar. 19, 1997 PCT Pub. No. WO97/35176 PCT Pub. Date Sep. 25, 1997Improvements in a biosensor of the type having reservoirs or wells for analyzing a biological liquid are disclosed. A biosensor (190) includes a waveguide (164) placed between a plurality of members such as plates (100, 186), at least one of the members (100) being formed to define the walls (132, 134, 136) of the reservoirs where the liquid is biologically analyzed. The walls of the reservoirs are made of an inert, opaque material such as a metal. Although the biosensor may include a gasket (162), the gasket is associated with the members and waveguide in such a way (e.g. by recessing the gasket into a channel formed into a metal plate) so that the gasket does not form any significant portion of the reservoir wall. Waveguides of varying composition (e.g. plastic, quartz or glass) may be associated with the members to form the biosensor. The metal plate of the biosensor has input and output ports for infusing, draining, or oscillating the liquid to be analyzed in the reaction reservoir. Also disclosed is a sled-shaped waveguide associated with a rear lens to couple light out of the waveguide to serve as a quality control measure thus insuring that the biosensor is properly placed and that the light is working.
Owner:UNIV OF UTAH RES FOUND

Methods and compositions for the manipulation and characterization of individual nucleic acid molecules

A method for observing and determining the size of individual molecules and for determining the weight distribution of a sample containing molecules of varying size, which involves placing a deformable or nondeformable molecule in a medium, subjecting the molecule to an external force, thereby causing conformational and / or positional changes, and then measuring these changes. Preferred ways to measure conformational and positional changes include: (1) determining the rate at which a deformable molecule returns to a relaxed state after termination of the external force, (2) determining the rate at which a molecule becomes oriented in a new direction when the direction of the perturbing force is changed, (3) determining the rate at which a molecule rotates, (4) measuring the length of a molecule, particularly when it is at least partially stretched, or (5) measuring at least one diameter of a spherical or ellipsoidal molecule. Measurements of relaxation, reorientation, and rotation rates, as well as length and diameter can be made using a light microscope connected to an image processor. Molecule relaxation, reorientation and rotation also can be determined using a microscope combined with a spectroscopic device. The invention is particularly useful for measuring polymer molecules, such as nucleic acids, and can be used to determine the size and map location of restriction digests. Breakage of large polymer molecules mounted on a microscope slide is prevented by condensing the molecules before mounting and unfolding the molecules after they have been placed in a matrix.
Owner:WISCONSIN ALUMNI RES FOUND

Image processing and analysis of individual nucleic acid molecules

A method for observing and determining the size of individual molecules and for determining the weight distribution of a sample containing molecules of varying size, which involves placing a deformable or nondeformable molecule in a medium, subjecting the molecule to an external force, thereby causing conformational and / or positional changes, and then measuring these changes. Preferred ways to measure conformational and positional changes include: (1) determining the rate at which a deformable molecule returns to a relaxed state after termination of the external force, (2) determining the rate at which a molecule becomes oriented in a new direction when the direction of the perturbing force is changed, (3) determining the rate at which a molecule rotates, (4) measuring the length of a molecule, particularly when it is at least partially stretched, or (5) measuring at least one diameter of a spherical or ellipsoidal molecule. Measurements of relaxation, reorientation, and rotation rates, as well as length and diameter can be made using a light microscope connected to an image processor. Molecule relaxation, reorientation and rotation also can be determined using a microscope combined with a spectroscopic device. The invention is particularly useful for measuring polymer molecules, such as nucleic acids, and can be used to determine the size and map location of restriction digests. Breakage of large polymer molecules mounted on a microscope slide is prevented by condensing the molecules before mounting and unfolding the molecules after they have been placed in a matrix.
Owner:WISCONSIN ALUMNI RES FOUND

Image processing and analysis of individual nucleic acid molecules

A method for observing and determining the size of individual molecules and for determining the weight distribution of a sample containing molecules of varying size, which involves placing a deformable or nondeformable molecule in a medium, subjecting the molecule to an external force, thereby causing conformational and / or positional changes, and then measuring these changes. Preferred ways to measure conformational and positional changes include: (1) determining the rate at which a deformable molecule returns to a relaxed state after termination of the external force, (2) determining the rate at which a molecule becomes oriented in a new direction when the direction of the perturbing force is changed, (3) determining the rate at which a molecule rotates, (4) measuring the length of a molecule, particularly when it is at least partially stretched, or (5) measuring at least one diameter of a spherical or ellipsoidal molecule. Measurements of relaxation, reorientation, and rotation rates, as well as length and diameter can be made using a light microscope connected to an image processor. Molecule relaxation, reorientation and rotation also can be determined using a microscope combined with a spectroscopic device. The invention is particularly useful for measuring polymer molecules, such as nucleic acids, and can be used to determine the size and map location of restriction digests. Breakage of large polymer molecules mounted on a microscope slide is prevented by condensing the molecules before mounting and unfolding the molecules after they have been placed in a matrix.
Owner:WISCONSIN ALUMNI RES FOUND

Method and apparatus for analyzing time series data

The present invention relates to a method and an apparatus for determining which one or more time series parameters of a plurality of time series parameters relating to operation of a system are correlated with a first operation state of the system. According to the invention, the method comprises providing time series data including data relating to a time series of each of the plurality of time series parameters; determining at least two first time periods, wherein the system is in the first operation state during the at least two first time periods; determining at least one second time period, wherein the system is in a second operation state during the at least one second time period; determining, for each respective time series parameter of the plurality of time series parameters, a first characteristic parameter relating to a first characteristic of the time series of the respective time series parameter for each of the at least two first time periods and the at least one second time period; and determining which one or more time series parameters of the plurality of time series parameters relating to the operation of the system are correlated with the first operation state of the system by determining, for each respective time series parameter of the plurality of time series parameters, whether or not the respective time series parameter is correlated with the first operation state of the system based on the first characteristic parameters of the respective time series parameter determined for each of the at least two first time periods and the at least one second time period.
Owner:EUROPEAN SPACE AGENCY
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