170results about "Imaging particle spectrometry" patented technology

A method and corresponding devices to reduce the presence of unwanted ions within the pseudo-potential well of the RF fields for inductively coupled plasma mass spectrometry is disclosed. This method reduces space-charge effects and leaves more room available for the ions of interest and, hence, leads to increased transmission of the desired ions into the mass analyzer. In the present invention, the space charge is reduced by adding a low-band resolving DC potential, or a fast sweeping resolving DC potential with a notch, or a fast constant resolving DC potential with a notch, or by adding auxiliary quadrupolar or dipolar RF potential to the quadrupole ion guide, such that all the unwanted ions will be outside the stable region and therefore ejected. This reduces the charge density in the ion beam and ameliorate space charge effects.

The present invention relates to the technical field of geomagnetic activities, and in particular to an auroral substorm simulation method and macroscopic model based on neutral atom imaging measurement. The method includes the following steps: establishing a neutral atom imaging simulation equation for dynamic evolution of ion flux distribution of a ring current guided by ion pitch angle diffusion; and using the neutral atom imaging simulation equation, forward modeling neutral atom simulation images in an ecliptic plane corresponding to different ion pitch angle distribution functions during a geomagnetically quiet period and during an auroral substorm period to reproduce ring current ion flux distribution patterns in a growth phase stage and a recovery phase stage of an auroral substorm process. The present invention can emulate and reproduce ring current ion flux distribution patterns in a growth phase stage and a recovery phase stage of an auroral substorm process, which lays a foundation for further research.

The invention relates to a device for analyzing a biological sample, comprising: a sample stage; a charged particle source configured to deliver a charged particle beam to a location on the sample stage; and a first laser source and a first focusing optic configured to direct the laser beam emitted by the first laser source toward the sample stage, where the charged particle source, the first laser source, and the first focusing optic are configured such that the charged particle beam and the laser beam are directed toward opposite sides of the sample stage.

Disclosed is a method of mass spectrometry comprising the steps of: providing a library of matrix data, said matrix data comprising one or more physico-chemical properties of one or more matrix components as a function of retention time; chromatographically separating a sample, said sample containing at least some of said matrix components and one or more analyte components; analysing said sample at a plurality of retention times to obtain sample data, said sample data comprising one or more physico-chemical properties of one or more sample components as a function of retention time; and calculating one or more error values as a function of retention time based on a comparison between said sample data and said matrix data.

The imaging mass analysis system of the present invention comprises: an imaging mass analysis unit (100) for collecting data by performing mass analysis on a plurality of micro-areas within a measurement area set on a target sample, and obtaining an image showing a distribution of signal intensity based on the data; a quantitative analysis unit (300) for performing analysis on the target sample or a similar sample by a prescribed analysis method showing higher quantitativeness than the mass analysis performed by the imaging mass analysis unit, and obtaining a quantitative value using the analysis result; and a processing unit (400) for obtaining a relationship between the signal intensity and the quantitative value based on the quantitative value and the signal intensity, and using the relationship to estimate a quantitative value at an arbitrary position within the signal intensity distribution. Thus, it is possible to reduce complicated and labor-intensive operations and obtain a high-precision concentration image corresponding to an MS imaging image.

A method and system for calculating the arrival time window of a plume at an analyzer is presented. The plume is generated from a tissue or geological sample by laser ablation. In some embodiments, external sensors measure ambient parameters such as temperature and pressure to more accurately calculate the arrival time window. In other embodiments, direct measurements of the plume's arrival time or non-contact measurements of the plume's flow velocity in a pipe are used to determine the arrival time window of the plume at the analyzer.

A needle for mass spectrometry having a solid shaft and a first end with a tip having a radius of curvature of less than 1500 nm, wherein the tip is distal from a second end of the needle, wherein the second end is configured to accept an electrical potential, and wherein the tip provides an ionization region when the electrical potential is applied. Methods of manufacturing a needle having a radius of curvature of less than 1500 nm. Methods and systems including at least one needle of the present disclosure for qualitative and / or quantitative analysis.

A method for the spectrometric analysis of sample material located in at least one ablation area 4 on a sample support 2 comprises: determining an ablation area dimension between opposing boundaries o

The present invention relates to a method for analyzing and measuring sample materials deposited on the surface of a sample holder, comprising: (a) defining a plurality of regions on the surface, some of which are in contact with the sample material, (b1) using a desorption beam to sample a sample segment on one region to generate desorbed molecules, which are ionized and transferred to an analyzer, (b2) scanning the region along a non-linear trajectory selected from a plurality of predefined non-linear trajectories on the region by changing the orientation of the beam relative to the surface, while keeping the holder in one position, (c) using a spatial adjustment device of the sample holder to transition from the scanned region to the next region to be scanned, and (d) repeating steps (b1), (b2) and (c) until a predetermined termination condition is met. The present invention also relates to a system for analyzing ions, which has an ion generating device and a control unit.

The invention relates to an imaging elemental analyzer for imaging one or more analyte elements in an organic sample, wherein the analyzer comprises: a chamber for receiving an organic sample containing one or more analyte elements to be imaged, wherein the internal chamber pressure surrounding the sample is in the range of 10 -5 up to 10 -2mbar; at least one irradiant selected from: (i) an ion gun for irradiating the sample with a high-intensity primary ion beam, wherein the primary ions are generated in the ion gun at a pressure below 1 mbar, the ion gun serving to focus the primary ion beam onto a localized spot on the sample surface and to move the spot over time to a plurality of locations on the sample surface; (ii) a laser for irradiating a localized spot on the sample surface and to move the spot over time to a plurality of locations on the sample surface; a gas-filled RF ion guide for receiving the generated ions, comprising the elements released from the sample in response to irradiation by the primary ions or the laser, wherein the RF ion guide prevents the propagation of any ions whose m / z values ​​are less than the mass or mass range of the analyte elements;and a time-of-flight (TOF) mass analyzer for recording the ions or reaction products of the secondary ions formed from the RF ion guidance, wherein the TOF mass analyzer is configured for a repetition rate of at least 5 kHz. The invention also relates to a corresponding method and a corresponding device for imaging one or more inorganic, non-volatile elements.

Methods and systems to calculate the arrival time window of a plume at an analyzer is presented. The plume is generated from a tissue or geological sample by laser ablation. In some embodiments, an external sensor measures the ambient parameters such as temperature and pressure to calculate the arrival time window more accurately. In other embodiments, direct measurement of plume arrival times or non-contact measurement of the flow speed of the plume in a tube are used to determine the arrival time window of the plume at an analyzer.

Systems and methods for image and / or video processing of mass spectrometry data comprise a processor in communication with a storage device, and computer system code executed by the processor that causes the processor to perform an image or video analysis comparison between a second data plot image or video to a first data plot image or video, and generate a delta dataset based on the comparison. The delta dataset is representative of the differences between the first and second data plots. In additional systems and methods the computer system code can perform an image or video analysis comparison between the first and second data plots and a reference data plot, generate first and second delta datasets based on the comparisons, which are representative of the differences between the first and second data plots and the reference data plot, and perform a statistical analysis on the first and second delta datasets.

The invention generally relates to methods for analyzing a tissue sample. In certain aspects, the invention provides methods that involve obtaining a tissue sample including an unsaturated compound, conducting a radical reaction on the tissue sample that targets a carbon- carbon double bond within the unsaturated compound to thereby produce a plurality of compound isomers, subjecting the plurality of compound isomers to mass spectrometry analysis to identify a location of the carbon-carbon double bond within the unsaturated compound, and quantifying the plurality of compound isomers in order to distinguish normal tissue from diseased tissue.

The invention generally relates to mass spectral analysis. In certain embodiments, methods of the invention involve analyzing a lipid containing sample using a mass spectrometry technique, in which the technique utilizes a liquid phase that does not destroy native tissue morphology during analysis.

The present invention relates to an apparatus and method for performing a desorption scan on an analyte material placed on a sample carrier, which may include the following operating modes: (a) setting the position of the carrier to be close to the impact area at which the beam is aligned for local desorption of the analyte material; (b) determining the actual position of the carrier after setting the position; (c) comparing the determined actual position with the target position of the carrier to determine any deviation; (d) if a deviation is detected, adjusting the beam orientation so that the beam is aligned to the impact area that would have been produced on the carrier in the absence of a deviation; (e) applying the beam to the impact area to locally desorb the analyte material and convey it to an analyzer; and (f) checking whether a predetermined end condition is satisfied, and if not, repeating steps (a)-(e) for subsequent non-conforming impact areas.

To provide a data analysis method, a data analysis device, and an analyzer that automatically execute difference analysis between samples differing in shape and size, without performing troublesome image deformation processing.SOLUTION: Provided is a data analysis method for equipment analyses that acquires, using a computer, object information related to a difference between a plurality of samples. The method executes: a computation processing step S3 in which persistent homology processing is performed on data that includes the signal value of an m-dimensional array structure (m=an integer of 2 to n, inclusive) that is obtained from one data group and a persistent diagram is created, for each of a plurality of data groups; and analysis processing steps S4, S5, S7, S8 in which the persistent diagrams obtained respectively from the plurality of data groups are compared, and object information is obtained, on the basis of the difference of dispersion state of plots in the whole of the persistent diagram to be compared and / or information about plots whose positions cannot be mapped in the persistent diagram to be compared.SELECTED DRAWING: Figure 2

The invention relates to an apparatus for multimodal analysis of sample material, for example from a tissue, which detects molecular image information from the sample material in a spatially resolved manner, for example using a MALDI time-of-flight mass analyser, records light microscopy image information from the sample material in a spatially resolved manner, and associates the two with improved accuracy to give spatially resolved co-registered overall image information.

Immunohistochemistry (INC) and in situ hybridization (ISH) methods are used for the targeted detection and localization of biomolecules (e.g., proteins and miRNAs) in tissues or cells, for research applications and clinical applications such as those of pathologists (e.g., biomarker analysis of resected tumors or tumor biopsies). These methods include mass spectrometry imaging (MSI) as a mode for detecting and localizing biomolecules in tissues or cells. The methods employ optically cleavable mass-tagged reagents linked to probes such as antibodies and nucleic acids and used to enable multiplexed immunohistochemistry and in situ hybridization, with MSI as the detection / readout mode. The methods also include multi-omics MSI procedures in which MSI of optically cleavable mass-tagged probes is combined with other MSI modes, such as direct label-free MSI of endogenous biomolecules from biological samples (e.g., tissues).

[0006] Embodiments of the present invention relate to replacing previous ICP-based ionization systems with new laser ionization systems, and provide improved mass spectrometer-based devices and methods for using them to analyze samples, particularly the use of mass spectrometry mass cytometry, imaging mass spectrometry, and imaging mass cytometry for the analysis of biological samples. Accordingly, embodiments of the present invention provide a device, e.g., a mass cytometer, that includes: (1) a sampler; (2) a laser ionization system that receives material removed from the sample by the sampler, the laser ionization system including an ionization system conduit and a pulsed laser adapted to ionize sample material passing through or exiting the ionization system conduit; and (3) a mass spectrometer that receives elemental ions from the ionization system and analyzes the elemental ions.

A method and apparatus for monitoring and / or controlling the extent of denaturation and / or bond cleavages of proteins on any surface (e.g., biological tissues, biofilms, etc.). In one embodiment, a low power laser (e.g., a 5 mW, 362 nm diode laser) is directed through a biological sample to a photodetector. The sample is heated by a set of radiant heaters to between about 220° C. and about 250° C. in a time period of between 10 seconds to 60 seconds. The baseline transmissivity of the sample is monitored continuously throughout treatment of the biological sample via continuous monitoring of the signal voltage detected at the photodetector. Upon detection of increase in relative transmissivity in the biological sample, the heating treatment is concluded and the biological sample is removed for in situ protein identification as part of an imaging MALDI-MS measurement.

One mode of an imaging analysis apparatus according to the present invention includes: a measurement unit configured to acquire analysis result data by performing ion mobility mass spectrometry for each of a plurality of micro regions in a predetermined measurement region in a sample; a correlation investigation unit (22, 23) configured to investigate a correlation between an m / z and an ion mobility in the analysis result data based on the analysis result data acquired by the measurement unit; and a data reduction unit (24, 25) configured to reduce an amount of the analysis result data by limiting an ion mobility range according to the m / z or limiting an m / z range according to the ion mobility based on the correlation obtained by the correlation investigation unit.

An MS2 analysis for one precursor ion is performed to collect data on each micro area within a measurement target area (S1). A plurality of product ions are extracted based on those data (S2), and a mass spectrometric (MS) imaging graphic is created for each m / z of the product ion (S3). Hierarchical cluster analysis is performed on the created MS imaging graphics to group the product ions based on the similarity of the graphics (S4). Product ions having similar distributions are sorted into the same group. Such a group of ions can be considered to have originated from the same compound. Accordingly, the intensity information of a plurality of product ions is totaled in each group and for each micro area (S5), and an MS imaging graphic is created based on the totaled intensity information (S6). Even if there are a plurality of compounds overlapping the precursor ion, the influence of the overlapping can be eliminated through those steps. Thus, a graphic having a higher level of SN ratio, sensitivity and dynamic range than an MS imaging graphic obtained at a single product ion can be created and displayed.

According to one aspect of the present invention, an analysis method using MALD I-MS and LA-ICP-MS both capable of performing imaging mass analysis comprises: a step (S2) for setting a region (A) on a sample coated with a substrate; a step (S3) for performing mass analysis on each of the plurality of minute regions in the region A using MALD I-MS; a step (S4) for setting, on the sample, a region (B) including the entire region (A) and a region in which the matrix is present, the region (B) being different from the region (A); a step (S5) for performing mass analysis on each of the plurality of minute regions in the region B using LA-ICP-MS; a step (S6) for determining the presence or absence of an influence of the matrix-related component on the basis of data within the region A and data of a range outside the region A generated by LA-ICP-MS; and a step (S7) of correcting, as necessary, the signal strength in the data obtained in (S5) on the basis of the determination result.

A multiplex method for co-detecting a plurality of different types of biomarkers in a tissue sample on a single slide comprises: (a) providing a tissue sample on a single slide; (b) performing antigen

The present invention relates to a method for single-cell imaging mass spectrometry (MS) by correlating an optical image of a cell sample with an MS image. The method of the invention is in particular useful in research to test concomitantly optical and molecular phenotypes at a single-cell resolution.

An imager 13 takes an image of a sample-placement surface of a sample plate 5. A plate identifier 103 decodes a barcode on the image to acquire a plate ID, recognizes the well arrangement, and retrieves a template having a cell arrangement corresponding to the well arrangement from a plate information storage section 101. A sample presence identifier 104 identifies, from the image, a well on which a sample is placed. A plate correspondence information input assistant 105 creates a template image in which the background color of the cells is changed depending on the presence of the sample, and displays it on a display unit 12. An analysis operator inputs information into each cell by a direct character-input operation or a drag-and-drop operation from a previously created sample list. The inputted information is associated with the corresponding well number and stored in a registration information storage section 107.

Disclosed herein is a method of analysing a tissue sample. The method comprises identifying one or more regions of interest within the tissue sample based on a tissue stain that has been applied to the tissue sample. Analyte material is then generated from the one or more regions of interest identified based on the tissue stain using a direct surface sampling probe (10), which analyte material is then received at a sampling inlet (30) and passed towards a mass and / or ion mobility spectrometer (50) for analysis.

A method for ion mapping of a sample, which has the following features: Application of a liquid sample to a metallic target surface, Separation of components of the liquid sample on the metallic target surface according to one or more physicochemical properties in a first spatial dimension and in a second spatial dimension and Ionizing and mass-analyzing multiple separated areas of the separated sample to generate an ion map of at least a portion of the sample separated on the target surface, wherein the components of the sample are applied to the target surface and separated by mechanical, hydrodynamic and / or aerodynamic means, and wherein the one or more physicochemical properties include one or more from the group comprising surface binding, surface affinity, solubility, binding affinity and polarity.

A SIMS-based analysis system, that includes (a) a memory circuit that is configured to store (i) an estimated model of a non-planar three dimensional (3D) structural element, and (ii) a measured SIMS spectrum of the 3D structural element; (b) a calibration information circuit that is configured to obtain calibration information, (c) a SIMS simulating circuit that is configured to provide a (i) an updated model of the 3D structural element, the updated model is indicative of changes undergone by the 3D structural element during the sputtering process, and (ii) a simulated SIMS spectrum of the 3D structural element; and (d) a fitting circuit that is configured to determine at least one of geometry and material concentration parameters based on the simulated SIMS spectrum and the measured SIMS spectrum.

A computer-implemented method for detecting at least one analyte in a sample with a laser desorption mass spectrometer (220) is disclosed. The method comprises:a) at least one imaging step comprising imaging at least one reflective target (128) by using at least one imaging device (235), wherein the sample comprising the at least one analyte is applied to the reflective target (128);b) at least one sample recognition step comprising localizing at least one sample region on the reflective target (128); andc) at least one analyte detection step comprising detecting the at least one analyte in the sample using surface assisted laser desorption ionization mass spectrometry (SALDI-MS) with the laser desorption mass spectrometer (220), wherein laser irradiation is applied to the reflective target (128) by using at least one laser source (222) of the laser desorption mass spectrometer (220) such that at least one ion of the at least one analyte is generated which is detected by using at least one of a mass analyzing unit (224) or an ion-mobility spectrometry device of the laser desorption mass spectrometer (220), wherein the laser irradiation is steered on the localized sample region by using at least one control device (237).