4results about "Imaging particle spectrometry" patented technology

A method for the spectrometric analysis of sample material located in at least one ablation area 4 on a sample support 2 comprises: determining an ablation area dimension between opposing boundaries o

An MS2 analysis for one precursor ion is performed to collect data on each micro area within a measurement target area (S1). A plurality of product ions are extracted based on those data (S2), and a mass spectrometric (MS) imaging graphic is created for each m / z of the product ion (S3). Hierarchical cluster analysis is performed on the created MS imaging graphics to group the product ions based on the similarity of the graphics (S4). Product ions having similar distributions are sorted into the same group. Such a group of ions can be considered to have originated from the same compound. Accordingly, the intensity information of a plurality of product ions is totaled in each group and for each micro area (S5), and an MS imaging graphic is created based on the totaled intensity information (S6). Even if there are a plurality of compounds overlapping the precursor ion, the influence of the overlapping can be eliminated through those steps. Thus, a graphic having a higher level of SN ratio, sensitivity and dynamic range than an MS imaging graphic obtained at a single product ion can be created and displayed.

To process data determined from image charge / current signals representing multiple ions of a given charge state (Q), each undergoing vibrational motion at respective oscillation frequencies (f) within an ion analysis apparatus.SOLUTION: A data set includes a measured signal frequency (f0) common to a plurality of measured image charge / current signals, and a plurality of estimated ion charge values corresponding to the respective amplitudes of the measured image charge / current signals. Using an integer charge value ([Qi]) corresponding to one of the estimated ion charge values rounded to the nearest integer, a plurality of different image charge / current signal frequency candidate values (fiCand) is calculated. The plurality of calculated image charge / current signal frequency candidate values (fiCand) is compared with the plurality of different signal frequencies (f) of the measured image charge / current signals to measure a score value representing the similarity between them on the basis of the comparison.SELECTED DRAWING: Figure 2b

In one embodiment, a method for imaging a biological specimen is disclosed, which includes defining a plurality of reference points across the surface of the biological specimen; directing ablation radiation to the biological specimen at a first plurality of specimen locations corresponding to a first subset of the defined reference points, thereby ablating at least a portion of the specimen at each of the locations, thereby generating a gas-phase sample from each of the specimen locations; ionizing each of the gas-phase samples to generate ions corresponding to the gas-phase sample; acquiring one or more mass signals corresponding to the ions associated with each of the ablated portions; and identifying at least one region of interest based on the analysis of the mass signals. An image of the identified region of interest can be acquired using image mass cytometry.