98 results about "Tissue imaging" patented technology

The invention discloses a method for improving the axial resolution of photoacoustic microscopic imaging obtained based on a single-pixel frequency domain. The method comprises the following steps: 1) creating a Fourier substrate pattern with the spatial frequency of k in the axial direction; 2) respectively irradiating four Fourier substrate patterns with the spatial frequency of k and the initial phases of # imgabs0 #, # imgabs1 #, # imgabs2 # and # imgabs3 # to an imaging object, carrying out photoacoustic microscopic imaging, and acquiring data by using an ultrasonic transducer to obtain a response value of the ultrasonic transducer; 3) according to the obtained response value of the ultrasonic transducer, adopting a four-step phase shift method to obtain a Fourier coefficient corresponding to the spatial frequency k; and 4) gradually increasing the spatial frequency of the Fourier substrate pattern to the cut-off frequency of the objective lens, further obtaining Fourier coefficients corresponding to all spatial frequencies, obtaining a complete Fourier spectrum, and performing inverse Fourier transform to obtain a photoacoustic microscopic image after the axial resolution is increased. The method disclosed by the invention is expected to promote wider application of photoacoustic microscopic imaging in living cell and tissue imaging.

Provided herein are imaging systems for a patient including an imaging probe and an imaging assembly. Methods of performing an imaging procedure are also provided. The imaging probe includes: a first elongate shaft; a second elongate shaft positioned within a lumen of the first elongate shaft; a rotatable optical core positioned within a lumen of the second elongate shaft; and an optical assembly positioned proximate a distal end of the rotatable optical core. The optical assembly can direct light to tissue to be imaged and to collect reflected light from the tissue to be imaged. The imaging assembly can emit light into the imaging probe and receive the reflected light collected by the optical assembly.

A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

The invention discloses a wound tissue imaging and auxiliary diagnosis system, which belongs to the technical field of wound tissue detection and comprises a hardware platform and an upper computer system. The hardware platform comprises a data acquisition module, a preprocessing and fusion module and a communication and control module; and the upper computer system is used for receiving the protocol packet and carrying out data analysis, coordinate system alignment and multispectral image waveband registration. According to the wound tissue imaging and auxiliary diagnosis system, a heterogeneous computing architecture is adopted, FPGA assembly line processing and ARM control are combined, power consumption and the size are remarkably reduced, and high-precision wound detection and real-time decision support are achieved through multispectral and three-dimensional data fusion, hardware acceleration preprocessing and AI driving analysis.

A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

The present disclosure relates to techniques for imaging tissue of a subject. An exemplary method comprises transitioning the liquid crystal light shutter to the closed state to prevent the global shutter imager from receiving visible light; illuminating the tissue of the subject with the fluorescence excitation illumination source to accumulate charge at a plurality of pixels of the global shutter imager, while the liquid crystal light shutter is in the closed state; reading a set of accumulated charge at the plurality of pixels of the global shutter imager to produce a set of imaging data; and generating a fluorescence image frame based on the set of imaging data.

A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

The invention relates to the field of high-end ultrasonic transducer manufacturing, and particularly discloses a patch type ultrasonic transducer for deep tissue imaging and an acoustic material preparation method, and a backing preparation method comprises the following steps: weighing an adhesive, a metal filler and a non-metal additive according to a mass ratio of 15: 55-75: 2; fully and uniformly mixing the weighed raw materials, and putting the mixed sample into a molding mold capable of demolding; the pressure is maintained for 10-30 min under the conditions that the pressure is larger than 10 MPa and the plasticizing temperature of the adhesive is reached, then the heating function is turned off, when the temperature is reduced to 120 DEG C, the pressure is rapidly reduced to 0 MPa at the moment, the adhesive is taken out when the adhesive is naturally cooled to the room temperature, and the backing is obtained. The problems of high sound attenuation, low sound impedance and low sound velocity preparation of the backing are solved, the dilemma problem of low sound impedance and ultrahigh sound attenuation is effectively solved, and the problem that the ultrathin design of the wearable patch type ultrasonic transducer conflicts with the maintenance of excellent acoustic performance due to insufficient sound attenuation of the existing backing is effectively solved.

The invention relates to the technical field of biological medicine, in particular to an RNF / CLR / Cas / crRNA compound as well as a preparation method and application thereof. The RNF / CLR / Cas / crRNA compound disclosed by the invention is composed of an RNA nanoflower (RNF), a circular fluorescent reporter molecule (CLR) and a Cas / crRNA compound, and the circular fluorescent reporter molecule and the Cas / crRNA compound are combined on the RNA nanoflower through base complementary pairing; the Cas / crRNA compound is composed of a Cas protein and crRNA, and the crRNA is used for specifically recognizing the crRNA of a nucleic acid molecule. The RNF / CLR / Cas / crRNA compound can be used for nucleic acid molecule specificity detection and nucleic acid molecule base mutation detection, can also be used for fluorescence in situ hybridization tissue imaging, and has a good application prospect.

A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

The invention belongs to the technical field of biomedical engineering measurement, and discloses a diffused light tissue imaging blood oxygen-blood flow synchronous and same-position acquisition device which comprises a three-wavelength near-infrared laser, a plurality of multimode optical fibers, a first optical switch, a second optical switch, a plurality of Y-shaped optical fibers, a DOS / DOT photon detector module and a DCS / DCT photon detector module. The output end of the three-wavelength near-infrared laser is connected with one end of each multi-mode optical fiber through the first optical switch, and the other end of each multi-mode optical fiber is arranged on the surface of a measured medium; the common end of each Y-shaped optical fiber is arranged on the surface of a detected medium, a first branch port of each Y-shaped optical fiber is connected with the DOS / DOT photon detector module through the second optical switch, and a second branch port of each Y-shaped optical fiber is connected with the DCS / DCT photon detector module through the second optical switch. According to the invention, DOS / DOT and DCS / DCT are fused, simultaneous and same-position acquisition of tissue blood flow and blood oxygen signals is realized, the dimension of clinical information measured by a diffused light technology is widened, and the accuracy of oxygen metabolism rate calculation is improved.

The invention relates to the technical field of medical instruments, in particular to a three-mode endoscope system, a control method and an endoscopic surgery robotic.The three-mode endoscope system comprises a light source device, a mode switching assembly, an imaging handle and an image processing host; the mode switching assembly generates an observation mode switching signal; the light source device outputs visible light, first near-infrared light and second near-infrared light. The imaging handle transmits light output by the light source device to target tissue; the imaging handle collects an imaging light beam returned by the target tissue and outputs a corresponding electric signal; the image processing host receives the electric signal and generates a visual image. According to the three-mode endoscope system, a white light mode, a fluorescence mode and a blood flow mode are integrated in a single endoscope system, an operator can obtain a blood vessel map at a target tissue through the blood flow mode of the three-mode endoscope system, and therefore the trend of large blood vessels invisible to naked eyes is revealed in the dissection process, the risk of accidental injury of the blood vessels is avoided, and the dissection efficiency is improved. And the operation safety is improved.

The application discloses a terahertz biological tissue imaging system and method based on a metasurface antenna. The terahertz biological tissue imaging system comprises a terahertz signal generator, an intelligent system control device, a terahertz signal transmitter and a data acquisition module. The terahertz signal generator is used for generating an ultra-wideband terahertz wave signal. The intelligent system control device controls the terahertz signal generator to continuously generate the ultra-wideband terahertz wave signal. The terahertz signal transmitter is used for transmitting the ultra-wideband terahertz wave signal after programmable modulation to a biological tissue organ. The terahertz signal receiver is used for receiving terahertz biological signals scattered inside and around the biological tissue organ, and modulating the received terahertz biological signals. The data acquisition module is used for acquiring the terahertz biological signals. The intelligent sensing signal processing module is used for processing the terahertz biological signals to obtain a terahertz image of the biological tissue organ. The application solves the problems of narrow bandwidth and low gain in the corresponding technology.

Methods and systems for positioning and positioning a patient for irradiation therapy without fiducial markers. Platform settings including parameters of the patient support platform are obtained. Platform position data is obtained in which a patient is placed on a platform for imaging without fiducial markers, the platform position data including 3D coordinates of the platform relative to an imager coordinate system calibrated to a predefined fixed set of initialized coordinates in a treatment room. Target tissue is imaged with the patient on the platform. A treatment plan is generated based on the target tissue imaging. The treatment plan comprises a treatment radiation field of a treatment angle, and the treatment angle comprises a platform positioning parameter. When treatment is initiated, the patient is placed on the platform without fiducial markers according to the platform settings. In accordance with the treatment plan, the platform position data, and a coordinate system transformation of the imager coordinate system to the treatment room coordinate system, the platform is adjusted to position the patient at the placement position in the treatment room such that the target tissue is positioned at the isocenter of the treatment room.

Certain exemplary ultrasound probe method and / or apparatus embodiments are described, which can be used for 2D and / or 3D imaging soft tissue in an intra oral cavity.

The invention discloses a rare earth homologous dual-emission high-resolution deep imaging method based on deep learning enhancement. A plurality of long-life intermediate states of lanthanide upconversion nanoparticles (UCNPs) are utilized, a single 980nm pumping source is used for exciting Tm < 3 + > / Yb < 3 + > co-doped UCNPs, and two-photon fluorescence with higher penetrability and four-photon fluorescence with higher resolution are induced at the same time. By combining a dual-mechanism CycleGAN artificial neural network based on antagonism training and cyclic consistency constraint, cross-domain mapping between two fluorescence signals is established, and the advantages of two fluorescence imaging are cooperatively utilized, so that deep penetration and high-resolution imaging are realized. According to the invention, in a self-established wide-field microscopic imaging system, the resolution of 209nm of a single particle in a 808nm fluorescence channel can be realized, and the signal-to-background ratio is doubled. According to the invention, the technical bottleneck that the resolution and the penetration depth of a traditional multi-photon microscope in deep tissue imaging are mutually restricted is effectively solved.

The application discloses a pyridine cation mitochondrial fluorescent probe, the chemical name of the probe is 4-(8-dimethylamino-5-benzocoumarin)-methylpyridine iodine salt, and the chemical structural formula is shown as formula (I). The application further discloses application of the fluorescent probe in labeling or imaging display of mitochondrial distribution or morphology in living cells and in long-range tracking display of mitochondria in living cells and imaging display of nucleoli in cells. Experiments prove that the labeled mitochondrial fluorescent probe has high light bleaching resistance and a high fluorescence quantum yield, can realize long-range tracking of mitochondria in living cells, and can label fixed nucleoli in cells. It is indicated that the fluorescent probe has potential application and development value in the fields of fluorescent probes and tissue imaging, and has a wide application prospect.

An embedding mold is provided. Wherein the embedding mold comprises a bottom plate, a side surrounding plate, a side baffle and a supporting piece, the side surrounding plate is arranged on the bottom plate, and a containing cavity with a side opening is defined by the side surrounding plate; the side baffle is detachably combined with the side coaming at the side opening to form a shell structure with an embedding groove; the supporting piece is detachably mounted on the shell structure, can extend into the embedding groove from the outside of the shell structure, and is used for supporting the biological tissue to be embedded, so that the biological tissue to be embedded is suspended in the shell structure. The embedding sample obtained by virtue of the embedding mold can be stable in the whole slicing process, and the position of the biological tissue is relatively fixed, so that the imaging section integrity difference caused by the position difference of the biological tissue can be reduced, and the section imaging integrity after continuous slicing can be improved; and thus, complete and clear whole-tissue imaging can be obtained.

In accordance with an aspect of the present disclosure, a tissue imaging system includes a cassette configured to retain a tissue for imaging. The cassette includes a first cassette portion and a second cassette portion. The first cassette portion includes a recess formed in a central portion thereof, a window formed within the recess, and a first connection mechanism affixed thereto. The second cassette portion includes a cavity configured to compress a tissue therein and a second connection mechanism affixed thereto. An imaging device is configured to image the tissue through the window. Aligning the first connection mechanism and the second connection mechanism causes the first cassette portion to couple to the second cassette portion, compressing the tissue into the cavity for imaging.

The present disclosure provides a tissue imaging method, including: inserting an electronic probe into a lesion area of a patient and ablating tissue of the lesion area; capturing a first image including the lesion area by using an imaging apparatus; vibrating the electronic probe to generate displacement of at least a portion of the tissue of the lesion area, and capturing a second image including the lesion area by using the imaging apparatus; generating a correlation image according to a correlation between the first image and the second image; and computing an ablation boundary according to the correlation image.

A multiplexing adaptive optics method for deep-tissue imaging is provided, comprising: providing a first optical beam and a group of second optical beams, each with lower intensity and distinct modulation frequencies; combining the beams with temporal overlap to form a composite excitation beam; focusing the composite beam into a sample such that the second optical beams are laterally separated in the focal plane; detecting a signal excited by the composite beam; demodulating the signal with reference frequencies to compute phase and amplitude data at each modulation frequency and obtain a second signal; performing spatial measurements of the first beam relative to the second beams to derive down-sampled electric-field point spread functions; and merging the functions to reconstruct a full-sampled point spread function for identifying and correcting optical aberrations within the sample.

Systems and methods for motion estimation and correction in nuclear imaging systems are disclosed. In some examples, a processing device receives measurement data, such as positron emission tomography (PET) measurement data, from an image scanning system. The processing device applies a histo-imaging process to the measurement data and, based on applying the histo-imaging process to the measurement data, generates a histo-image. Further, the processing device applies a trained machine learning process to the histo-image and, based on applying the trained machine learning process to the histo-image, the processing device generates a short-frame image. The processing device also determines a position of a mass in the short-frame image, and generates motion data characterizing a motion of the mass based on the position. In some examples, a reconstructed image is generated based on the motion data.

The invention discloses an ultra-stable red fluorescent protein. The fluorescent protein disclosed by the invention is a protein with an amino acid sequence SEQ ID No.4. Experiments prove that the fluorescent protein has excellent electron microscope sample preparation resistance, can retain more fluorescent signals after osmic acid treatment, and can tolerate embedding of Epon resin; meanwhile, the fluorescent protein has excellent thermal stability, and can retain more fluorescent signals at 90 DEG C; in addition, the fluorescent protein has high chemical stability and light stability. Based on the related properties of the fluorescent protein, the fluorescent protein can be used in the fields of protein labeling, various types of fluorescence imaging and super-resolution photoelectric correlated imaging, and is used for tracking the structural and morphological changes of samples such as proteins, subcellular organelles, local cell regions and cells; the kit can be used for packaging related viruses or transgenic animals, marking specific proteins, specific organelles or specific cells, and realizing rapid transparent tissue imaging and expansion super-resolution imaging.

A system and method for in vivo tomographic tissue imaging using coded aperture X-ray scatter tomography are disclosed. The imaging system includes a coded aperture for spatially encoding X-ray scatter originating from within a patient. An X-ray detector array records the modulated scatter signal, which is analyzed and used to generate a spatially resolved X-ray scatter spectral reconstruction of the tissue and produce a spatially resolved scatter tissue image of the irradiated tissue.

A method for optimizing stimulation for a patient having a stimulator device, such as a deep brain stimulation (DBS) device, is disclosed that involves taking into account tissue imaging information in the environment around a lead. Test stimulation is provided at initial combinations of lead position and stimulation parameters, such as amplitude values, wherein the patient is scored for each combination. One or more objective criteria are measured and scored. The objective criteria may be electrode impedance and / or pressure indicators, such as cardiac signals. The process is iteratively repeated until a stop criterion is met. The lead position in question may be a longitudinal or rotational position about the lead, and if the lead is essentially oriented, preferably both.

The invention relates to a glandular cystitis operation imaging interference processing method, and relates to the technical field of medical image processing. The method comprises the following steps: constructing a tissue imaging repair neural network, wherein a tissue imaging repair model comprises a variational auto-encoder for generating an ambient light map, a repair network for generating a repair image, and a transmission rate estimation network for generating a transmission rate map; a reconstruction loss function is constructed based on the scattering model, the reconstruction loss function guides all parts of the tissue imaging repair model to be jointly trained, so that the tissue imaging repair neural network follows a modeling interference forming process of a physical rule according to the scattering model, and it is ensured that interference removal conforms to the glandular cystitis operation environment optical rule; in addition to reconstruction loss, the loss function further comprises atmospheric light prior loss followed by a variational auto-encoder in the initial training stage, S and V loss of an HSV space restoration image and restoration image consistency loss. The restored image output by the network can be closer to reality, and the organization structure is clearer.

The tissue imaging system, incorporates an integrated X-Ray and Ultrasound Imaging system. The X-Ray system is used for imaging and generating a 3D Volume of the extracted tissue sample (specimen). The Ultrasound imaging system is used for imaging the body area of interest of the patient from which the tissue sample is to be extracted. On the display of the system, the physician draws contours on the area of interest and the same is displayed as a 3D Volume. A 3D volume of the extracted tissue is generated using the x-ray imaging sub-system. Quantitative analysis is performed on the two 3D volumes (extracted tumor x-ray imaging and the contoured ultrasound imaging) to determine the difference between the contoured and the surgically extracted specimen to assist the physician in determining if further surgical intervention is required.

The present disclosure relates to ultrasound-based strategies and techniques that are specially designed for time-resolved simultaneous cardiac tissue visualization and intracardiac flow instability mapping. First, to achieve high-resolution tissues images while also acquiring flow data, we devised an acquisition scheme which consists of two batches. The first batch is dedicated to tissue imaging, where we transmit a series of diverging waves emitted from different virtual source locations. These pulses have a single pulse-cycle to optimize axial resolution and are coherently compounded for improving lateral resolution. This paradigm generates tissue images with an improved resolution, allowing for real-time guidance at frame rates up to 50 fps. The second batch is dedicated for flow imaging where all diverging waves are transmitted from the same virtual source location. These flow pulses have a 5-cycle pulse length for improved flow signal. The length of this batch can be adjusted, but our experiments use a length of 64. The final transmission scheme alternates between the tissue batch and flow batch.

The invention relates to the technical field of medical detection. The invention aims to provide a nano-probe for detecting the expression of an epidermal growth factor receptor of head and neck squamous carcinoma cells. The nano-probe is a nano-particle with a core-shell structure, the nano particle takes a nano Au particle as a core, and 4-mercaptobenzoic acid (4-MBA) is adsorbed on the surface of the Au core as a Raman signal internal reference molecule; according to the nanoparticle, SiO2 is used as a shell, and PEG and EGFR antibodies are linked to the SiO2 shell. According to the nanoprobe, head and neck squamous cell cancer cell and tissue imaging is researched by adopting a surface enhanced Raman spectroscopy technology, tumor cell and tissue EGFR expression images are drawn, and quantitative analysis and research are carried out. A rapid, visual and accurate new tool is provided for clinically detecting the squamous cell carcinoma of the head and neck and the EGFR expression condition of the squamous cell carcinoma of the head and neck.