43 results about "Tissue imaging" patented technology

The present disclosure relates to techniques for imaging tissue of a subject. An exemplary method comprises transitioning the liquid crystal light shutter to the closed state to prevent the global shutter imager from receiving visible light; illuminating the tissue of the subject with the fluorescence excitation illumination source to accumulate charge at a plurality of pixels of the global shutter imager, while the liquid crystal light shutter is in the closed state; reading a set of accumulated charge at the plurality of pixels of the global shutter imager to produce a set of imaging data; and generating a fluorescence image frame based on the set of imaging data.

The application discloses a terahertz biological tissue imaging system and method based on a metasurface antenna. The terahertz biological tissue imaging system comprises a terahertz signal generator, an intelligent system control device, a terahertz signal transmitter and a data acquisition module. The terahertz signal generator is used for generating an ultra-wideband terahertz wave signal. The intelligent system control device controls the terahertz signal generator to continuously generate the ultra-wideband terahertz wave signal. The terahertz signal transmitter is used for transmitting the ultra-wideband terahertz wave signal after programmable modulation to a biological tissue organ. The terahertz signal receiver is used for receiving terahertz biological signals scattered inside and around the biological tissue organ, and modulating the received terahertz biological signals. The data acquisition module is used for acquiring the terahertz biological signals. The intelligent sensing signal processing module is used for processing the terahertz biological signals to obtain a terahertz image of the biological tissue organ. The application solves the problems of narrow bandwidth and low gain in the corresponding technology.

Methods and systems for positioning and positioning a patient for irradiation therapy without fiducial markers. Platform settings including parameters of the patient support platform are obtained. Platform position data is obtained in which a patient is placed on a platform for imaging without fiducial markers, the platform position data including 3D coordinates of the platform relative to an imager coordinate system calibrated to a predefined fixed set of initialized coordinates in a treatment room. Target tissue is imaged with the patient on the platform. A treatment plan is generated based on the target tissue imaging. The treatment plan comprises a treatment radiation field of a treatment angle, and the treatment angle comprises a platform positioning parameter. When treatment is initiated, the patient is placed on the platform without fiducial markers according to the platform settings. In accordance with the treatment plan, the platform position data, and a coordinate system transformation of the imager coordinate system to the treatment room coordinate system, the platform is adjusted to position the patient at the placement position in the treatment room such that the target tissue is positioned at the isocenter of the treatment room.

The application discloses a pyridine cation mitochondrial fluorescent probe, the chemical name of the probe is 4-(8-dimethylamino-5-benzocoumarin)-methylpyridine iodine salt, and the chemical structural formula is shown as formula (I). The application further discloses application of the fluorescent probe in labeling or imaging display of mitochondrial distribution or morphology in living cells and in long-range tracking display of mitochondria in living cells and imaging display of nucleoli in cells. Experiments prove that the labeled mitochondrial fluorescent probe has high light bleaching resistance and a high fluorescence quantum yield, can realize long-range tracking of mitochondria in living cells, and can label fixed nucleoli in cells. It is indicated that the fluorescent probe has potential application and development value in the fields of fluorescent probes and tissue imaging, and has a wide application prospect.

An embedding mold is provided. Wherein the embedding mold comprises a bottom plate, a side surrounding plate, a side baffle and a supporting piece, the side surrounding plate is arranged on the bottom plate, and a containing cavity with a side opening is defined by the side surrounding plate; the side baffle is detachably combined with the side coaming at the side opening to form a shell structure with an embedding groove; the supporting piece is detachably mounted on the shell structure, can extend into the embedding groove from the outside of the shell structure, and is used for supporting the biological tissue to be embedded, so that the biological tissue to be embedded is suspended in the shell structure. The embedding sample obtained by virtue of the embedding mold can be stable in the whole slicing process, and the position of the biological tissue is relatively fixed, so that the imaging section integrity difference caused by the position difference of the biological tissue can be reduced, and the section imaging integrity after continuous slicing can be improved; and thus, complete and clear whole-tissue imaging can be obtained.

A multiplexing adaptive optics method for deep-tissue imaging is provided, comprising: providing a first optical beam and a group of second optical beams, each with lower intensity and distinct modulation frequencies; combining the beams with temporal overlap to form a composite excitation beam; focusing the composite beam into a sample such that the second optical beams are laterally separated in the focal plane; detecting a signal excited by the composite beam; demodulating the signal with reference frequencies to compute phase and amplitude data at each modulation frequency and obtain a second signal; performing spatial measurements of the first beam relative to the second beams to derive down-sampled electric-field point spread functions; and merging the functions to reconstruct a full-sampled point spread function for identifying and correcting optical aberrations within the sample.

Systems and methods for motion estimation and correction in nuclear imaging systems are disclosed. In some examples, a processing device receives measurement data, such as positron emission tomography (PET) measurement data, from an image scanning system. The processing device applies a histo-imaging process to the measurement data and, based on applying the histo-imaging process to the measurement data, generates a histo-image. Further, the processing device applies a trained machine learning process to the histo-image and, based on applying the trained machine learning process to the histo-image, the processing device generates a short-frame image. The processing device also determines a position of a mass in the short-frame image, and generates motion data characterizing a motion of the mass based on the position. In some examples, a reconstructed image is generated based on the motion data.

A method for optimizing stimulation for a patient having a stimulator device, such as a deep brain stimulation (DBS) device, is disclosed that involves taking into account tissue imaging information in the environment around a lead. Test stimulation is provided at initial combinations of lead position and stimulation parameters, such as amplitude values, wherein the patient is scored for each combination. One or more objective criteria are measured and scored. The objective criteria may be electrode impedance and / or pressure indicators, such as cardiac signals. The process is iteratively repeated until a stop criterion is met. The lead position in question may be a longitudinal or rotational position about the lead, and if the lead is essentially oriented, preferably both.

The invention relates to a glandular cystitis operation imaging interference processing method, and relates to the technical field of medical image processing. The method comprises the following steps: constructing a tissue imaging repair neural network, wherein a tissue imaging repair model comprises a variational auto-encoder for generating an ambient light map, a repair network for generating a repair image, and a transmission rate estimation network for generating a transmission rate map; a reconstruction loss function is constructed based on the scattering model, the reconstruction loss function guides all parts of the tissue imaging repair model to be jointly trained, so that the tissue imaging repair neural network follows a modeling interference forming process of a physical rule according to the scattering model, and it is ensured that interference removal conforms to the glandular cystitis operation environment optical rule; in addition to reconstruction loss, the loss function further comprises atmospheric light prior loss followed by a variational auto-encoder in the initial training stage, S and V loss of an HSV space restoration image and restoration image consistency loss. The restored image output by the network can be closer to reality, and the organization structure is clearer.

The present disclosure relates to ultrasound-based strategies and techniques that are specially designed for time-resolved simultaneous cardiac tissue visualization and intracardiac flow instability mapping. First, to achieve high-resolution tissues images while also acquiring flow data, we devised an acquisition scheme which consists of two batches. The first batch is dedicated to tissue imaging, where we transmit a series of diverging waves emitted from different virtual source locations. These pulses have a single pulse-cycle to optimize axial resolution and are coherently compounded for improving lateral resolution. This paradigm generates tissue images with an improved resolution, allowing for real-time guidance at frame rates up to 50 fps. The second batch is dedicated for flow imaging where all diverging waves are transmitted from the same virtual source location. These flow pulses have a 5-cycle pulse length for improved flow signal. The length of this batch can be adjusted, but our experiments use a length of 64. The final transmission scheme alternates between the tissue batch and flow batch.

The application belongs to the technical field of medical imaging, and discloses a deep tissue blood oxygen saturation photoacoustic precise imaging method, which comprises the following steps: S1, obtaining a light flux distribution preliminary model of a to-be-measured region; S2, photoacoustic imaging is performed on the to-be-measured tissue to obtain original photoacoustic imaging data of the to-be-measured tissue; S3, calculating arterial photoacoustic signal intensity; S4, calculating the relative light flux distribution of each arterial region to form an arterial light flux space correction network; S5, introducing the arterial light flux space correction network into the light flux preliminary model to correct the light flux distribution preliminary model, and obtaining the corrected relative light flux distribution; and S6, calculating the blood oxygen saturation of the to-be-measured tissue in the region other than the artery according to the relative light flux of the artery and the to-be-measured tissue imaging region other than the artery, and obtaining the blood oxygen saturation distribution of the entire to-be-measured tissue imaging region. The application calibrates the light flux distribution in the deep tissue through the arterial photoacoustic signal, and realizes the precise measurement of the blood oxygen saturation of the deep tissue.

The invention belongs to the technical field of intelligent biological materials, and discloses a preparation method and application of a pH / Ca < 2 + > responsive near-infrared injectable hydrogel sensing stent. The problem that the current synthesized responsive hydrogel is non-injectable is effectively solved. Comprising the following steps: synthesizing PEA-MAA-DVB microgel, and functionalizing the PEA-MAA-DVB microgel with vinyl to obtain MG; activating the PEA-MAA-DVB microgel and DMTMM (Dimethyl Tetramethylbenzidine); the preparation method comprises the following steps: dissolving Cy5-NH2 and Cy5.5-NH2 in a phosphate buffer solution to obtain a dye solution; reacting a dye solution with the activated microgel dispersion to obtain NP; mG and NP are mixed, a photoinitiator and a NaOH aqueous solution are added, and MG-NP is obtained; and carrying out ultraviolet radiation on the MG-NP to obtain the Gels (MG-NP) hydrogel. The hydrogel can be injected, reversibly responds to pH and Ca < 2 + > concentration, and supports near-infrared tissue imaging.

Provided herein are probes, methods and kits for detecting target molecule, e.g., in a multiplex manner at a single-cell or tissue level.

To provide a nervous tissue imaging system that does not require fluorescent dyes, fluorescent tissue probes or other fluorescent markers to increase visual contrast between the nerve / dura mater and non-nervous tissue surrounding the nerve / dura mater.SOLUTION: The system includes a housing containing an excitation light source, optically coupled with a source optical train, where the excitation light source emits excitation light in a first wavelength range, which can be in a near ultraviolet light range, to illuminate a tissue region of interest including healthy nervous tissue and healthy non-nervous tissue. The excitation light is in the first wavelength range that causes the healthy nervous tissue, in response to being illuminated with the excitation light, to endogenously autofluoresce and emit first autofluorescence light at a first luminance in a second wavelength range. The healthy non-nervous tissue, in response to being illuminated with the excitation light, either avoids emitting any autofluorescence light in the second wavelength range, or endogenously autofluoresces and emits second autofluorescence light in the second wavelength range at a second luminance that is lower than 50%of the first luminance.SELECTED DRAWING: Figure 1

The invention provides a navigation grinding method and a surgical robot system for craniomaxillofacial abnormal proliferation bone fibers, and the method comprises the steps: S1, obtaining craniomaxillofacial bone tissue image data and intraoral scanning data of a target human body, and constructing a three-dimensional digital craniomaxillofacial model; s2, based on abnormal pathological features of abnormal proliferation bone fibers in the craniomaxillofacial bone tissue image data, identifying and obtaining a bone fiber abnormal proliferation disease lesion area, and generating a lesion area three-dimensional model; s3, human body key tissue structures existing in the craniomaxillofacial region are obtained through segmentation, the minimum distance between abnormally proliferated bone fibers in the bone fiber abnormal proliferation disease lesion region and any human body key tissue structure is calculated, and a distance field graph is generated; s4, based on the lesion area three-dimensional model and the distance field map, a navigation grinding path of the surgical robot is established, and therefore the functions of medical image data recognition and analysis, navigation grinding path automatic generation, robot-assisted surgery and the like based on the artificial intelligence technology are achieved.

A nervous tissue imaging system includes a pair of matched filters, including an excitation light filter that only passes excitation light in a first wavelength range from 365 nm to 400 nm, and a received light detection filter that only passes received light in a second wavelength range from 433 nm to 450 nm. An endoscope is connected with a first source optical train for guiding excitation light through the first source optical train and the excitation light filter to uniformly illuminate the excitation light on a patient's tissue region of interest inside an enclosed cavity of the patient's body. The endoscope is connected to a camera device and a receiving optical train and the received light detection filter for receiving and passing into the camera device light signals including autofluorescence light in the second wavelength range from healthy nervous tissue in the patient's tissue region of interest.

The present disclosure provides conjugates that comprise a small molecule attached to an antifouling nanoparticle. The present disclosure also provides methods of using such conjugates, such as diagnosis, tissue imaging, temporal monitoring, and treatment.

Systems and methods are provided for measuring the mechanical properties of ocular tissue, such as the lens or cornea, for both diagnosis and treatment monitoring. A laser blocking feedback system is included to achieve frequency accuracy and sensitivity, facilitating highly precise operations and diagnosis. Differential comparisons between regions of ocular tissue in a patient, whether in the same eye or the contralateral eye, can further facilitate early diagnosis and monitoring.

The embodiment of the invention is suitable for the technical field of medicine, and provides a physiological gating signal generation method and system.The method comprises the steps that a signal probe is controlled to scan a target area, and echo signals fed back by the target area are received; wherein the echo signal can reflect physiological motion information of target tissue in the target area; generating a motion image of the target tissue according to the echo signal, and displaying the motion image; determining a physiological gating signal of the target tissue based on the motion image; the physiological gating signal is used for controlling the medical equipment to perform imaging scanning on the target tissue. By adopting the method, high-quality physiological gating signals can be conveniently acquired, so that artifacts during target tissue imaging are reduced.

The present disclosure relates to techniques for imaging tissue of a subject. An exemplary method comprises transitioning the liquid crystal light shutter to the closed state to prevent the global shutter imager from receiving visible light; illuminating the tissue of the subject with the fluorescence excitation illumination source to accumulate charge at a plurality of pixels of the global shutter imager, while the liquid crystal light shutter is in the closed state; reading a set of accumulated charge at the plurality of pixels of the global shutter imager to produce a set of imaging data; and generating a fluorescence image frame based on the set of imaging data.

The invention belongs to the technical field of bioengineering and molecular imaging, and particularly relates to a novel multi-excitation fluorescent protein and application thereof, the nucleotide sequence of the novel multi-excitation fluorescent protein is shown as SEQ ID NO.1, and the amino acid sequence of the novel multi-excitation fluorescent protein is shown as SEQ ID NO.2. The multi-excitation fluorescent protein disclosed by the invention effectively overcomes the limitation that the protein or a light source needs to be frequently replaced due to narrow excitation spectrum in multiple imaging of the traditional fluorescent protein, so that multi-mode and multi-parameter imaging by using the same protein in a single experiment becomes possible. According to the invention, the experimental process is greatly simplified, the experimental cost is reduced, the application range of the method in the fields of dynamic tracking of living cells, simultaneous detection of multiple components, deep tissue imaging and the like is remarkably widened, and a more powerful and flexible tool is provided for life science research and medical diagnosis.

The application discloses a kind of synchrotron orthogonal linear polarization light resonant cavity light-emitting diode.The device, from bottom to top, is metal substrate, lower mirror, parallel plane resonant cavity, upper electrode, upper mirror;The epitaxial layer of the parallel plane resonant cavity includes n-type layer, p-type layer and active region;Wherein n-type layer, p-type layer and active region are made of birefringent semiconductor material, and the active region is birefringent quantum well structure.The application has the characteristics of simple structure, high integration, narrow spectral line width, adjustable spectral mode and the like, and has wide application prospect in the fields of precision measurement, microscopic tissue imaging, visual imaging, three-dimensional display, optical communication, quantum communication and the like.

An imaging system for multi-scale, non-contact US, PA, and FL imaging with a spatiotemporal sampling scheme, scanning with light integrated with mechanical mirror scanning pathway and testing instantaneous mechanical pressure and FL emission over target tissue surface. The imaging system provides deep and wide tissue imaging using a scanning laser vibrometry with broad tissue excitation using, for example, a tunable pulsed laser and fast microscopic imaging of identified regions of interest using an optic attachment to the camera to secure the tight focusing on the tissue surface. In some configurations, a raster¬ scanning photoacoustic imaging with a fast pulsed laser and parallelized data acquisition is used.

A method of generating an image of a medical instrument using a medical apparatus includes a catheter, a medical instrument assembly, and an imaging assembly. The catheter has a first working channel terminating with a first distal exit, and a second working channel terminating with a second distal exit. The medical instrument assembly has a medical instrument adapted to be housed within the first working channel and adapted to be extendable through the first distal exit to an extended position beyond the first distal exit to intercept a target tissue. The imaging assembly includes an imaging device adapted to be housed within the second working channel and is extendable through the second distal exit to an extended position beyond the second distal exit. The imaging device is adapted to generate an image in an image plane of the distal end of the medical instrument when the distal end of the medical instrument is extended out the first distal exit.

Systems, devices, and methods for enhanced tissue imaging and biochemical detection using near-infrared (NIR) illumination in conjunction with resonant structures, such as whispering gallery mode (WGM) resonators and dielectric waveguides are proposed that operate in either transmission mode—where NIR light passes through a target sample—or reflection mode—where reflected NIR signals are recoupled into the sensing structure. These configurations support low-cost, flexible, real-time, and high-sensitivity analysis across a broad spectrum of biological and non-biological specimens, thereby enabling applications in tissue diagnostics, immunoassay-based detection, and material characterization, including deployment in point-of-care and resource-limited environments.

A system and method for performing a dual energy subtraction imaging process on a fixed or mobile fluoroscopy imaging system for pulmonary imaging applications is provided. The dual energy subtraction image processing system includes a processor configured to operate a radiation source of a fluoroscopy imaging system in a pulsed manner to obtain a number of high energy (HE) frames, to form an HE image from the number of HE frames, to operate the radiation source of the fluoroscopy imaging system in a continuous manner to obtain a number of low energy (LE) frames, to form an LE image from the LE frames, and to employ the HE image and the LE image in a dual energy subtraction imaging process to output a number of enhanced contrast images. The processor is also configured to operate the detector in continuous manner to obtain the HE frames and the LE frames.

The utility model provides a fixing system for optical imaging of a living body. The fixing system comprises a main body structure, a bottom cover, a cover glass, a ventilation pipeline and an imaging assembly, the main body structure comprises a base and a peripheral fence, and a containing hole is formed in the base and used for containing a living organ or tissue to be fixed; the bottom cover is in threaded connection with the base, a square groove is formed in the upper end face of the bottom cover, and a round hole communicated with the square groove is formed in the lower end face; the cover glass is arranged in the square groove, and an adsorption cavity is formed between the cover glass and the living organ or tissue between the accommodating holes; the ventilation pipeline is used for exhausting air outwards to enable the adsorption cavity to form negative pressure, living organs or tissues are fixed, and the situation that an imaging focal plane is unstable due to shaking of a living body is avoided; the imaging assembly comprises an object carrier and imaging equipment, the object carrier is used for bearing the bottom cover, and the imaging equipment is used for imaging living organs or tissues. The fixing system provided by the utility model is low in cost, wide in adaptive objective lens range, adaptive to more types of living organs or tissues, and reusable.

A system and method for acquiring in vivo tomographic X-ray scatter data for tissue discrimination of breast tissue. The system includes a coded aperture for spatially encoding X-ray scatter originating from within a patient's breast. Detectors record the modulated scatter signal, which is used to reconstruct a spatially resolved estimate of the X-ray scatter spectra which can then be used to generate spatially resolved tissue type estimates for the user.

A system and method for in vivo tomographic tissue imaging using coded aperture X-ray scatter tomography are disclosed. The imaging system includes a coded aperture for spatially encoding X-ray scatter originating from within a patient. An X-ray detector array records the modulated scatter signal, which is analyzed and used to generate a spatially resolved X-ray scatter spectral reconstruction of the tissue and produce a spatially resolved scatter tissue image of the irradiated tissue.