10results about How to "Reduce phototoxicity" patented technology

The invention provides a single particle diffusion quantitative feature prediction method and device, electronic equipment and a storage medium. The method comprises the following steps: acquiring a target input image corresponding to a target single particle; based on a pre-trained particle trajectory prediction model, according to the target input image, predicting to obtain a particle pseudo trajectory image; on the basis of the particle pseudo-trajectory image, diffusion quantitative characteristics of the target single particle are calculated; wherein the particle trajectory prediction model is obtained by performing training optimization according to a first training sample set formed by the single-particle motion blurred image and the corresponding particle motion trajectory image. According to the method, the actual movement path of the target single particle is predicted from the target input image through the particle trajectory prediction model, and the diffusion quantitative characteristic of the target single particle is calculated based on the actual movement path, so that the diffusion coefficient of a large number of single particles can be rapidly and accurately predicted in a high-mark-density environment, and the phototoxicity is remarkably reduced; diffusion characteristics of different biological particles can be effectively distinguished, and a powerful tool is provided for dynamic research of particles in a diffusion system and living cells.

The invention discloses a single molecule positioning method based on high-speed phase-locked detection, which can perform high-precision single molecule positioning and imaging on a fluorescent sample under the condition of using a single laser light source. Molecular excitation of a sub-diffraction limit region is generated by using nonlinear characteristics of excitation and fluorescence emission when a fluorescence sample is excited by laser, and meanwhile, positioning information is obtained by surrounding a single molecule of a region of interest by using an illumination light beam; and the laser intensity is continuously improved, and the positioning precision is also improved until sub-five-nanometer precision information is obtained. And the detection end adopts a high-speed lock-in amplifier to lock fluorescence signal information of characteristic frequency in real time, and meanwhile, the final signal-to-background ratio is also improved. Compared with traditional high-precision positioning based on double laser light sources, the method has the advantages of a single laser light source, does not need special fluorescent dye, does not need special matching between double-wavelength lasers, and has universality; and the damage caused by phototoxicity and photobleaching due to over-high illumination intensity is avoided.

The invention discloses an immunofluorescence imaging method for driving tyramine signal amplification based on chemiluminescence and application thereof, and relates to the technical field of biomedical imaging. The immunofluorescence imaging method comprises the following steps: carrying out first incubation on a to-be-detected sample and a peroxidase-labeled antibody; performing second incubation on the to-be-detected sample after the first incubation and a tyramine substrate containing a fluorophore under a dark condition; adding a chemiluminescent substrate solution into the to-be-detected sample after the second incubation, and carrying out image acquisition; wherein the antibody can be specifically bound with a target protein; the tyramine substrate can generate active free radicals under the catalysis of peroxidase, so that the active free radicals are covalently cross-linked with tyrosine residues; the chemiluminescent substrate solution comprises hydrogen peroxide and luminol. The immunofluorescence imaging method improves imaging signal and fluorescence stability, effectively reduces background interference and phototoxicity, is suitable for long-time stable fluorescence immunoimaging, and is especially suitable for high-sensitivity detection of low-expression protein markers.

This invention belongs to the fields of biomedicine and fluorescent probe technology, and relates to a biheaded functional molecule and its application in the preparation of products for imaging cell tailpods or migration bodies. The biheaded functional molecule has the chemical structure shown in Formula I; wherein R is a hydrophilic positive ionic fluorescent group, X is methylene or O, Y is methylene or carbonyl, and n is 1~3. The biheaded functional molecule provided by this invention can achieve long-term cell membrane anchoring and can dynamically track the migration body formation process with low phototoxicity, low background fluorescence, high photostability, and high brightness.

The application discloses an intelligent visual projection method and system suitable for optical imaging, wherein the system comprises a visual stimulation control module for setting a visual stimulation control signal; an image output module for generating full-spectrum visual stimulation and light intensity according to the visual stimulation control signal; a spectrum switching module for receiving the full-spectrum visual stimulation and the light intensity and outputting first specific spectrum visual stimulation; an infrared light source module for outputting infrared spectrum visual stimulation; a spectrum processing module for filtering preset green spectrum signals of the first specific spectrum visual stimulation and integrating the infrared spectrum visual stimulation to output second specific spectrum visual stimulation; and an image stimulation focusing module for fine-tuning the projection area and light intensity of the second specific spectrum visual stimulation and projecting the second specific spectrum visual stimulation to a visual tissue region. The application can solve the problem that the prior art cannot meet the research demand of visual neural optical signals and has the characteristics of intelligence, automation, precision and no optical pollution.

This invention relates to an ultra-high cell density organ module based on click chemistry photocurable hydrogel, its construction method, and its applications. The construction method includes: providing norbornene-modified high molecular weight hyaluronic acid with a high grafting rate and thiol-modified high molecular weight hyaluronic acid; mixing a photoinitiator, cells, HANb solution, and HASH solution; and rapidly cross-linking and curing the module through a click chemistry reaction after extremely short-term light exposure to form the organ module. This invention utilizes the synergistic effect of the high molecular weight hyaluronic acid host material and the high grafting rate modification to achieve the construction of an ultra-high cell density organ module under ultra-low photoinitiator concentration and extremely short light exposure conditions. The liver module constructed based on this method possesses extremely high cell density, good mechanical properties, and excellent liver function.

The invention discloses a tumor targeting activatable nano photosensitizer as well as a preparation method and application thereof. The tumor targeting activatable nano photosensitizer comprises a cationic polymer, an anionic polymer and an aromatic structure photosensitizer. Cationic-pi interaction is formed between a cationic side chain in the cationic polymer and the photosensitizer with an aromatic structure, the nano photosensitizer is formed through self-assembly under the interaction, and the photosensitive activity of the nano photosensitizer in blood circulation is in a completely inactivated state; and no light toxic or side effect is generated on normal light exposure tissues. When selectively enriched in tumor tissues, the photosensitizer is structurally degraded in tumor cells in a specific environment, and the photosensitive activity of the photosensitizer is quickly activated and recovered, so that efficient tumor inhibition treatment is realized.

The present invention discloses a fluorescence loss principle of stimulated radiation loss sensitization and a super-resolution imaging method and device thereof, wherein, on the one hand, the fluorescence loss method uses near-infrared continuous laser to induce the stimulated radiation process of sensitized ions in rare-earth doped fluorescent nanoparticles, blocks the energy transfer process between the sensitized ions and one or more activated ions from the excitation source, quenches the luminescence of all energy levels of all activated ions, thereby realizing the simultaneous loss of fluorescence of different energy levels of different activated ions by a single beam loss laser. On the other hand, based on the loss method, a method and device for full-spectrum multicolor super-resolution imaging is realized, thereby performing full-spectrum multicolor super-resolution microscopic imaging of multiple luminescent ions or molecules based on the same pair of near-infrared wavelength excitation light / loss light, which has the advantages of no photobleaching, low phototoxicity, large depth, high resolution, low complexity, low cost, etc.

The invention provides a hypoxia-responsive protein degradation targeting chimera compound as well as a preparation method and application thereof. The structure of the compound is as shown in a formula (VII), and more specifically as shown in a formula (I). The compound disclosed by the invention takes an azo bond as a hypoxia response unit, can respond to a hypoxia tumor microenvironment and activate a protein degradation function of the hypoxia tumor microenvironment, and meanwhile, recovers a fluorescence signal of a photosensitizer so as to monitor drug release in real time. Under the irradiation of near-infrared light, the photosensitizer generates active oxygen to induce tumor cell apoptosis, so that photodynamic therapy and PROTAC combined therapy are realized. The compound provided by the invention can solve the problems of systemic toxicity after administration of an existing PROTACs drug system and poor effect in tumor treatment, and meanwhile, can monitor drug release in real time, so that the compound has good practical application value. # imgabs0 #