The invention provides a fluorescent probe represented by the formula (I), wherein R1 and R2 are independently selected from H or an aromatic vinyl group, and at least one of R1 and R2 is the aromatic vinyl group; R3 is selected from H, F, Cl, Br, OH, OCH3, N(CH3)2 or C1-C6 alkyl; and R4 is selected from C1-C6 alkyl. Compared with the prior art, because the fluorescent probe provided by the invention has a relatively large electronic conjugated system and plane, the intensity of the intramolecular charge transfer effect can affect the molecular fluorescence emission intensity; when the fluorescent probe and the G-quadruplex generate a specific action, the flexibility of intramolecular rotatable double bonds is restricted, the intramolecular charge transfer effect is enhanced, and the fluorescence is also enhanced significantly; moreover, the fluorescent probe provided by the invention has the advantages of low biotoxicity, phototoxicity and photobleaching property, good light stability, good water solubility and good cell membrane permeability.