5results about How to "Reduce phototoxicity" patented technology

The invention discloses an immunofluorescence imaging method for driving tyramine signal amplification based on chemiluminescence and application thereof, and relates to the technical field of biomedical imaging. The immunofluorescence imaging method comprises the following steps: carrying out first incubation on a to-be-detected sample and a peroxidase-labeled antibody; performing second incubation on the to-be-detected sample after the first incubation and a tyramine substrate containing a fluorophore under a dark condition; adding a chemiluminescent substrate solution into the to-be-detected sample after the second incubation, and carrying out image acquisition; wherein the antibody can be specifically bound with a target protein; the tyramine substrate can generate active free radicals under the catalysis of peroxidase, so that the active free radicals are covalently cross-linked with tyrosine residues; the chemiluminescent substrate solution comprises hydrogen peroxide and luminol. The immunofluorescence imaging method improves imaging signal and fluorescence stability, effectively reduces background interference and phototoxicity, is suitable for long-time stable fluorescence immunoimaging, and is especially suitable for high-sensitivity detection of low-expression protein markers.

This invention belongs to the fields of biomedicine and fluorescent probe technology, and relates to a biheaded functional molecule and its application in the preparation of products for imaging cell tailpods or migration bodies. The biheaded functional molecule has the chemical structure shown in Formula I; wherein R is a hydrophilic positive ionic fluorescent group, X is methylene or O, Y is methylene or carbonyl, and n is 1~3. The biheaded functional molecule provided by this invention can achieve long-term cell membrane anchoring and can dynamically track the migration body formation process with low phototoxicity, low background fluorescence, high photostability, and high brightness.

The application discloses an intelligent visual projection method and system suitable for optical imaging, wherein the system comprises a visual stimulation control module for setting a visual stimulation control signal; an image output module for generating full-spectrum visual stimulation and light intensity according to the visual stimulation control signal; a spectrum switching module for receiving the full-spectrum visual stimulation and the light intensity and outputting first specific spectrum visual stimulation; an infrared light source module for outputting infrared spectrum visual stimulation; a spectrum processing module for filtering preset green spectrum signals of the first specific spectrum visual stimulation and integrating the infrared spectrum visual stimulation to output second specific spectrum visual stimulation; and an image stimulation focusing module for fine-tuning the projection area and light intensity of the second specific spectrum visual stimulation and projecting the second specific spectrum visual stimulation to a visual tissue region. The application can solve the problem that the prior art cannot meet the research demand of visual neural optical signals and has the characteristics of intelligence, automation, precision and no optical pollution.

This invention relates to an ultra-high cell density organ module based on click chemistry photocurable hydrogel, its construction method, and its applications. The construction method includes: providing norbornene-modified high molecular weight hyaluronic acid with a high grafting rate and thiol-modified high molecular weight hyaluronic acid; mixing a photoinitiator, cells, HANb solution, and HASH solution; and rapidly cross-linking and curing the module through a click chemistry reaction after extremely short-term light exposure to form the organ module. This invention utilizes the synergistic effect of the high molecular weight hyaluronic acid host material and the high grafting rate modification to achieve the construction of an ultra-high cell density organ module under ultra-low photoinitiator concentration and extremely short light exposure conditions. The liver module constructed based on this method possesses extremely high cell density, good mechanical properties, and excellent liver function.