18 results about "Candida krusei" patented technology

The invention relates to the technical field of culture media, and particularly discloses a candida chromogenic culture medium as well as a preparation method and application thereof. The candida chromogenic culture medium disclosed by the invention is prepared from the following components in concentration: 10 to 11 g / L of peptone, 12 to 17 g / L of agar, 7 to 9 g / L of glucose, 1 to 2 g / L of chromogenic substrate and 0.4 to 0.6 g / L of bacteriostatic agent, the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D-galactosamine and 4-methylumbelliferone-beta-D-N-acetyl galactosamine, and the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D- The bacteriostatic agent is prepared by mixing chloramphenicol, taurocholic acid and nalidixic acid. When being used for detecting and identifying candida albicans, candida tropicalis, candida krusei and candida glabrata, the chromogenic culture medium provided by the invention has strong specificity, short identification time and wide practical application value.

This invention discloses the application of larvicin or its pharmaceutical salt in the preparation of antifungal drugs. The structure of larvicin is shown below. This invention discovers an effective antifungal compound with pharmaceutical potential: larvicin. The compound larvicin of this invention exhibits clear antifungal activity against pathogenic fungi such as *Candida albicans*, *Candida auris*, *Candida tropicalis*, *Candida parapsilosis*, *Candida krusei*, *Cryptococcus neoformans*, *Trichophyton mentagrophytes*, and *Trichophyton rubrum*, with a minimum inhibitory concentration (MIC). 90 The range is 2–8 μg / mL.

This invention discloses a fermentation method for improving the flavor and stability of old dough, belonging to the field of food technology. The method includes: mixing activated *Candida cruzi*, *Lactobacillus brevis*, *Hansenula polymorpha*, *Yarrowia lipolytica*, and *Saccharomyces cerevisiae* in a specific ratio to obtain a compound bacterial solution; and fermenting coarse grains with this compound bacterial solution to obtain coarse grain steamed buns. This invention employs the synergistic fermentation of five specific bacterial strains. *Lactobacillus brevis* and *Candida cruzi* synergistically optimize the rheological properties of the dough, *Hansenula polymorpha* and *Saccharomyces cerevisiae* synergistically produce rich aroma compounds, and *Yarrowia lipolytica* secretes phytase and cellolytic enzymes to lower the glycemic index. The coarse grain steamed buns prepared by this invention contain 55-65 flavor compounds, and the glycemic index is estimated to be reduced to 48-56, achieving a stable and controllable fermentation process, a low-GI product, and a rich flavor.

The present invention discloses a plant essential oil composition having anti-Candida activity and a preparation method thereof. The composition comprises a clove light oil component and a cinnamon heavy oil component in a volume ratio of 1:1 to 128:1. Through extensive experimental studies, the present invention has found that the combination of undistilled and refined clove essential oil and cinnamon essential oil does not have a good anti-Candida effect and lacks a significant synergistic effect. However, the combination of a clove light oil component obtained by secondary distillation and refined clove and a cinnamon heavy oil component in a volume ratio of 1:1, 8:1, or 16:1 has a synergistic anti-Candida effect, particularly against Candida tropicalis, Candida krusei, and Candida albicans. The composition can be used to prepare an anti-Candida drug.

This application relates to the field of biotechnology, and particularly to primer pairs, primer-probe compositions, PCR premixes, kits, and methods for detecting multiple respiratory pathogens. Nucleic acid sequences are shown in primer pairs as indicated by SEQ ID NO: 1-2, 4-5, 7-8, 10-11, 13-14, 16-17, 19-20, 22-23, 25-26, 28-29, 31-32, 34-35, 37-38, and 40-41. For target respiratory pathogens, this application designs specific amplification primer pairs, paired with suitable probes, enabling the simultaneous detection of eight targets. Furthermore, the detection process avoids interference from heme, trimethoprim, sulfamethoxazole, amphotericin B, itraconazole, fluconazole, azithromycin, adrenaline, and lidocaine hydrochloride in the test sample. It exhibits good anti-interference properties and shows no cross-reactivity with *Rhodococcus equi*, *Candida tropicalis*, and *Candida krusei*.

This invention relates to a fermented beverage and its preparation method, belonging to the field of fermented beverage technology, with IPC classification number A23L2 / 38. The invention uses wolfberry, cassia seed, burdock root, mulberry bark, and codonopsis as raw materials. These are boiled in water, and then inoculated with specific strains of *Candida krusei* CICC 31807, *Saccharomyces cerevisiae* CICC 31105, and *Bifidobacterium animalis* CICC 6165 in a ratio of (2-5):1:(2.5-6) ​​for fermentation. After post-processing, a concentrated material is obtained. This concentrated material is then combined with *Phyllanthus emblica* extract, mulberry extract, sweetener, acidity regulator, excipients, and water to prepare the fermented beverage. The beverage prepared by this invention has good stability, maintaining good stability even after exposure to high or low temperatures; it has a rich aroma, no bitterness, and a smooth, pleasant taste, providing a refreshing and delicate experience, and also has a certain liver-protective effect.

The present application relates to the technical field of microorganism, and particularly relates to a Dvorst's bacteria and a method for fermenting corn steep liquor by using the same.The present application provides a Dvorst's bacteria capable of efficiently degrading vomitoxin in corn steep liquor, and corn steep liquor is fermented by using the Dvorst's bacteria and Candida krusei in cooperation, so that the pH of the corn steep liquor can be increased, the content of vomitoxin and anti-nutritional factors in the corn steep liquor can be reduced, and the content of choline in the corn steep liquor can be significantly increased, the quality of the corn steep liquor is obviously improved, the corn steep liquor after the cooperative fermentation can be better applied in the field of animal feed, and has a wide application prospect.

The invention relates to the technical field of biological medicine, in particular to an antifungal pharmaceutical composition based on a (1, 3)-beta-D-glucan synthase inhibitor. In-vitro experiment results show that the antibacterial peptide LL-37 and (1, 3)-beta-D-glucan synthase inhibitors (caspofungin, micafungin, anidulafungin and iremifungin) are combined for use to achieve the effect of synergistically inhibiting candida albicans, candida auricula, candida tropicalis, candida glabrata, candida parapsilosis and candida krusei, so that the antibacterial peptide LL-37 can be combined with the (1, 3)-beta-D-glucan synthase inhibitors (caspofungin, micafungin, anidulafungin and iremifungin) to achieve the effect of inhibiting the (1, 3)-beta-D-glucan synthase inhibitors). And 3) preparing the antifungal drug by combining the 1, 2, 4, 5, 6, 7, 8, 8, 9, 10- However, the antibacterial peptide LL-37 is easily degraded by in-vivo protease, so that the expression of the antibacterial peptide LL-37 in an organism is up-regulated by adopting an antibacterial peptide LL-37 expression accelerant, and the antibacterial peptide LL-37 and a (1, 3)-beta-D-glucan synthetase inhibitor are synergistically antibacterial in the organism.

The invention belongs to the technical field of in-vitro molecular detection of pathogenic microorganisms, and particularly provides a primer probe group and a reagent for multiple fluorescent PCR (Polymerase Chain Reaction) and / or multiple quantitative digital PCR detection of five candida. The primer probe group comprises specific primer pairs and corresponding probes aiming at candida albicans, candida tropicalis, candida glabrata, candida parapsilosis and candida krusei. Based on the unified combination, two compatible detection paths are established: five-target synchronous qualitative detection can be realized by adopting a multiple fluorescent PCR (Polymerase Chain Reaction) method, and five-target absolute quantitative analysis can also be realized by adopting a multiple quantitative digital PCR method; the two methods can be applied independently or jointly. Through verification, the primer probe group has high specificity; the sensitivity is excellent, and DNA extracted from a clinical sample can be directly detected. The method is simple in detection process and short in time consumption, and an efficient and reliable molecular detection solution is provided for early differential diagnosis and precise medication of clinical invasive candidiasis.

The present invention relates to the technical field of microbial fermentation, and particularly relates to an auxiliary lipid-lowering composition, a preparation method thereof, and an application thereof. A preparation method of an auxiliary lipid-lowering composition includes Step 1: First, mix apples, white hyacinth beans, red grapes, euryale ferox seeds, and lilies, then add water to form a slurry, and sterilize to obtain a fermentation substrate; Step 2: Inoculate Trichoderma reesei and ferment to obtain an initial fermented product; Step 3: Inoculate Candida krusei and ferment to obtain a second fermented product; Step 4: Take the supernatant of the second fermented product and inoculate Bifidobacterium breve, and ferment to obtain a third fermented product; Step 5: Take the third fermented product, filter and sterilize it to obtain an auxiliary lipid-lowering fermented product; Step 6: Mix the auxiliary lipid-lowering fermented product, freeze-dried natto powder, and ginkgo leaf extract evenly to obtain an auxiliary lipid-lowering composition. The auxiliary lipid-lowering composition of the present application has both safety and high bioavailability.

The invention discloses a method for improving methane production through anaerobic digestion of cellulose based on in-situ fermentation, and belongs to the technical field of development and utilization of agricultural waste biomass energy. The method comprises the following steps: (1) stirring ferric trichloride hexahydrate, ferrous chloride tetrahydrate and a sodium hydroxide solution in a 55-65 DEG C water bath to prepare ferroferric oxide; (2) Candida krusei is activated, a Candida culture solution is obtained, and the OD600 of the Candida culture solution is not lower than 1.0; (3) the candida culture solution is added into a PBS buffer solution containing 0.8-1.2 g / L of ferroferric oxide according to the volume ratio of 4-6%, then the mixture is added into a glutaraldehyde aqueous solution according to the volume ratio of (0.5-1.5): (0.5-1.5), the concentration of the glutaraldehyde aqueous solution is 2-3%, crosslinking is conducted at the room temperature for at least 2 h, and the yeast immobilized microbial inoculum is prepared; and (4) adding the immobilized microbial agent into an anaerobic digestion system for anaerobic digestion. According to the method, in-situ fermentation is assisted by an immobilization technology, so that the activity of yeast in a reactor is improved, alcohol is stably produced, and direct interspecific electron transfer is promoted, thereby improving the methane production efficiency of anaerobic digestion.

This application belongs to the field of microbial technology, specifically disclosing the application of *Lactobacillus fermentum* BLF03 in anti-Candida activity. *Lactobacillus fermentum* BLF03 was deposited on November 6, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36521. This application proposes a *Lactobacillus fermentum* BLF03 strain resistant to *Candida albicans* and *Candida krusei*, which can significantly inhibit the growth, reproduction, and hyphal transformation of *Candida albicans* and *Candida krusei* at the in vivo level, alleviate Candida-induced infection symptoms, reduce the expression level of the inflammatory marker procalcitonin (PCT) after Candida infection, improve the inflammatory stress response induced by infection, and has a significant protective effect against tissue damage caused by Candida invasion. It also has targeted preventive and adjunctive therapeutic effects on Candida infections in the urogenital tract and other sites, providing a new, safe, and highly effective strain for anti-Candida products.

The application discloses a heavy Zanthoxylum bungeanum essential oil component with antibacterial activity and a preparation method thereof. The Zanthoxylum bungeanum essential oil is deeply researched, and the Zanthoxylum bungeanum essential oil is separated into a light Zanthoxylum bungeanum essential oil component and the heavy Zanthoxylum bungeanum essential oil component through a molecular distillation instrument. The antibacterial and antifungal experimental results show that the heavy Zanthoxylum bungeanum essential oil component obtained through distillation refining has better bactericidal activity on Klebsiella pneumoniae, methicillin-resistant Staphylococcus aureus, Staphylococcus aureus and corynebacterium striatum compared with the Zanthoxylum bungeanum essential oil and the light Zanthoxylum bungeanum essential oil component, and has better bactericidal activity on Candida tropicalis, Candida krusei, Candida glabrata and white Candida, and a good technical effect is achieved.

The application discloses a primer probe combination, a kit and application for detecting pathogenic microorganisms in a genital tract, wherein the primer probe combination comprises primer probe sequences shown in SEQ ID NO. 1-SEQ ID NO. 96, the pathogenic microorganisms in the genital tract include 27 pathogenic microorganisms which can cause diseases in the genital tract, such as Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Candida albicans, Candida glabrata, Candida tropicalis, Candida auris, Candida krusei, Candida dubliniensis and the like, and four common vaginal lactobacilli, such as Lactobacillus crispatus, Lactobacillus jensenii, Lactobacillus gasseri and Lactobacillus iners, and further include a human internal standard. Based on the fluorescence quantitative PCR technology, the 27 pathogenic microorganisms which can cause diseases in the genital tract and the four common vaginal lactobacilli can be simultaneously detected in an in-vitro sample, the main pathogenic microorganisms of the genital tract infection can be covered, the evaluation of the vaginal ecological environment can be satisfied, the detection time is shortened to about 40 minutes, and the specificity and the sensitivity are good.

The present invention relates to new active agents for use in the treatment of diseases caused by the yeast strains C. albicans ATCC 10231, C. krusei ATCC 6258, and C. tropicalis Y-12968.

The invention belongs to the technical field of biology, and particularly relates to application of rCK14-3-3 protein in preparation of a vaccine for resisting Candida krusei infection, and the amino acid sequence of the rCK14-3-3 protein is shown as SEQ ID NO: 2. According to the invention, the Candida krusei recombinant 14-3-3 protein is used for immunizing a mouse after being compatible with a Freund's adjuvant for the first time, and the immunological effect of the Candida krusei recombinant 14-3-3 protein is evaluated, so that the Candida krusei recombinant 14-3-3 protein is proved for the first time to be capable of inducing humoral immunity and cellular immune response, and has good immunogenicity; meanwhile, the capability of resisting Candida krusei infection of the body can be improved, and a foundation is laid for further research of genetic engineering recombinant vaccines.

The invention relates to an essence preparation method, in particular to a method for extracting high-quality cigarette essential oil with assistance of fungus fermentation, and belongs to the IPC (International Patent Classification) C11B9 range. The method comprises the following steps: crushing plant raw materials, carrying out anaerobic fermentation by adopting candida krusei CICC 31796 and lactobacillus delbrueckii subsp. Lactis GDMCC NO.1. 3694 as zymophyte, then filtering, adding ethyl acetate for extraction, removing water, and concentrating to remove ethyl acetate, so as to obtain the essential oil. The essential oil prepared in a fermentation extraction mode is high in fragrance intensity and has a good application prospect in cigarettes; in addition, an essence finished product prepared from the essential oil is excellent in performance and high in stability.

A compound with broad-spectrum antibacterial activity and its antibacterial composition are provided. The compound with broad-spectrum antibacterial activity has formula (I), which are antibacterial drug source compounds with a broad antibacterial spectrum, good antibacterial activity, suitable in vivo pharmacokinetics, and significant in vivo therapeutic effects. Preferred compound BAB159 has good antibacterial activity against Staphylococcus, Clostridium perfringens, Enterococcus, Bacillus, Streptococcus, Haemophilus, Candida krusei, Aspergillus niger, and Trichophyton, which is comparable or better than the listed drugs. The combination of preferred compound BAB159 and polymyxin E exhibits significantly enhanced synergistic antibacterial activity, especially against multidrug-resistant strains.