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Candida drusei LSA and application thereof in ammonia-nitrogen degradation

A Candida and ammonia nitrogen degradation technology, applied in the field of microorganisms, can solve the problems of high operating costs, ammonia nitrogen, ammonia gas emissions, secondary pollution, etc., and achieve the effects of improving the environment in the house, improving safety, and increasing utilization

Active Publication Date: 2015-09-02
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing measures to control ammonia pollution in farms use physical methods (such as strengthening the ventilation in the house, building excrement and urine separation facilities, etc.), the operating cost is high, and because the discharge of ammonia nitrogen and ammonia to the surrounding environment has not been fundamentally eliminated, it is still a problem. It will cause secondary pollution, and the use of microbial methods can overcome the defects of the above methods, so it has a high application value in the treatment of ammonia and ammonia nitrogen pollution in animal husbandry

Method used

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  • Candida drusei LSA and application thereof in ammonia-nitrogen degradation
  • Candida drusei LSA and application thereof in ammonia-nitrogen degradation
  • Candida drusei LSA and application thereof in ammonia-nitrogen degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Isolation, purification and identification of Candida krusei LSA

[0028] 1. Isolation and purification of Candida krusei LSA

[0029] 1. Enrichment of ammonia nitrogen degrading bacteria

[0030] Take 20 g of chicken manure samples, inoculate them in a conical flask filled with 100 ml of sterilized distilled water (add sterilized glass beads to the bottle), shake at 30°C and 150 r / min for 30 minutes to fully break up the bacteria micelles, and let stand for 20 minutes. Take 5ml of the supernatant, inoculate it into a conical flask containing 100ml of enrichment medium, culture it with shaking at 30°C and 150r / min for 2 days, take 1ml of the culture medium and inoculate it again into a new enrichment medium, and repeat continuously Enriched for 10 generations.

[0031] 2. Screening, isolation and purification of ammonia nitrogen degrading bacteria

[0032] Draw 1ml of the enriched culture solution, and after 10 -1 After serial dilution, 200 μl was spread ...

Embodiment 2

[0043] Example 2, research on the function of Candida krusei LSA to degrade ammonia nitrogen

[0044] 1. Research on the degradation characteristics of Candida krusei LSA to high concentration ammonia nitrogen

[0045] 1. Preparation of Medium A

[0046] Medium A: Glucose was used as the sole carbon source, and the carbon source and (NH 4 ) 2 S0 4 The concentration of ammonia nitrogen is 1200mg / L, C / N is 10, and pH is 7.0.

[0047] 2. Pick a single clone of Candida krusei LSA, inoculate it in 100mL YPD liquid medium, and culture it with shaking at 30°C and 150r / min for 24-36h (to make the cell concentration in the culture system reach 8.0-9.0×10 8 cfu / mL), centrifuged at 4000rpm for 10min, collected the bacteria, and washed 3 times with sterile saline.

[0048] 3. Take the bacterial cells obtained in step 2 and resuspend them with sterile physiological saline to obtain a bacterial concentration of 8.0×10 8 cfu / mL bacterial suspension.

[0049] 4. Inoculate 0.1mL of the ...

Embodiment 3

[0074] Example 3, the influence of Candida krusei LSA on the amount of feces ammonia emission

[0075] 1. Pick a single clone of Candida krusei LSA, inoculate it in 100mL YPD liquid medium, and cultivate it with shaking at 30°C and 150r / min for 24-36h (to make the cell concentration in the culture system reach 8.0-9.0×10 8 cfu / mL), centrifuged at 4000rpm for 10min, collected the bacteria, and washed 3 times with sterile saline.

[0076] 2. Take the bacterial cells obtained in step 1 and resuspend them with sterile physiological saline to obtain a bacterial concentration of 8.0×10 8 cfu / mL bacterial suspension.

[0077]3. Take a large beaker (1L), put a small beaker in the large beaker (the small beaker is equipped with 20ml of absorption liquid; the absorption liquid is 0.01mol / L sulfuric acid solution), put 100g of fresh chicken manure in the large beaker The sample and 10ml of the bacterial suspension obtained in step 2 (using an equal volume of sterile normal saline as th...

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Abstract

The invention discloses a candida drusei LSA and an application thereof in ammonia-nitrogen degradation. The preservation registration number of the candida drusei LSA disclosed by the invention is CGMCC NO.10562. The invention further discloses a culture of the candida drusei LSA, and further discloses a bacterial suspension of the candida drusei LSA. The invention further discloses a product; and the active components of the product are the candida drusei LSA, the culture or the bacterial suspension. The product has the following function: (a) degrading ammonia and nitrogen, or (b) degrading ammonia and nitrogen in water, or (c) degrading ammonia and nitrogen in excrements, or (d) reducing the ammonia emission of the excrements. The candida drusei disclosed by the invention can be applied to the following aspects: (1) improvement of water quality problem of ammonia-nitrogen pollution, (2) improvement of indoor environmental health when the candida drusei is applied to livestock and poultry houses, and (3) application as a feed additive for chicken.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Candida krusei and the application of the candida krusei in degrading ammonia nitrogen. Background technique [0002] The waste water and feces produced in the process of livestock and poultry breeding will produce a large amount of malodorous gas during the storage process, among which the harm of ammonia gas is the most obvious. The lighter ones induce the occurrence of respiratory diseases in poultry, pigs and other animals, causing animal stress reactions, and the severe ones Those who can cause poisoning death. At the same time, a large amount of ammonia emission promotes the formation of particulate matter (PM2.5) that can enter the lungs, and diffuses into the atmosphere to react with some acidic substances to form ammonium salt aerosols, leading to eutrophication and acidification of the ecosystem. In addition, if the livestock and poultry breedi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A62D3/02C02F9/14C12R1/72
Inventor 刘国华刘志云谢庆石鹏君蔡辉益常文环张姝郑爱娟
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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