1460 results about "Feed additive" patented technology

The invention discloses a feed additive ratio control method and system based on environmental perception. The method comprises the steps that breeding environment, feed characteristics, equipment operation and animal physiological parameters are collected, and a high-quality parameter set is generated after preprocessing; calculating an environment stress index based on a multi-factor data fusion algorithm and analyzing a change trend; combining historical and current data, using time sequence prediction and a machine learning algorithm to predict additive requirements and generating an optimal proportioning strategy; a control instruction is generated according to the strategy, and the additive conveying and spraying process is regulated and controlled; and a matching strategy and control parameters are corrected in real time through closed-loop feedback, so that accurate regulation and control are realized. The device can dynamically adapt to environmental changes, reduce animal stress, and improve mixing uniformity and nutrition retention effect.

Provided herein, inter alia, are ruminant feed or feed additive compositions, and methods of using these feed or feed additive compositions to reduce methane emissions in ruminants while improving animal performance, these feed or feed additive compositions comprise one or more compounds that increase hydrogen production in ruminants, such as, but not limited to, 3-nitroxypropanol (3NOP) and one or more acetogens.

The invention relates to the technical field of compound fertilizers, and discloses a boron-zinc-molybdenum synergistic compound fertilizer, which is prepared from the following raw materials in parts by weight: calcium nitrate, ammonium polyphosphate, potassium sulfate, magnesium sulfate, disodium octoborate tetrahydrate, sugar alcohol chelated zinc, sodium molybdate and a synergistic auxiliary agent, the feed additive is prepared from a feed additive, an activator, 5-alginic acid oligosaccharide, aminolevulinic acid, sugar alcohol chelated magnesium, an antagonist, a stress-resistant enhancer and a coating material. According to the boron-zinc-molybdenum synergistic interaction compound fertilizer and the preparation process thereof, the disodium octoborate tetrahydrate, the sugar alcohol chelated zinc and the sodium molybdate are compounded according to a specific proportion, antagonism is avoided through proportion limitation, the synergistic effect of the three elements is remarkably enhanced, and the problem that a single element is insufficient or excessive is solved; chelated elements such as sugar alcohol chelated zinc and sugar alcohol chelated magnesium are combined with an activating agent (humic acid chelated calcium and silica sol) to form a gel-state chelate, so that the trace elements can be prevented from being fixed by soil, and the transfer efficiency of the trace elements in crops is improved.

The invention relates to the technical field of feed ingredient optimization, in particular to an ingredient optimization and adjustment system for a phage type antibiotic-free feed additive. Comprising the following steps: acquiring multi-dimensional environmental data and antibody content indexes and stress state information of individuals; determining a health index according to the antibody content index and the stress state information; performing batching delay analysis according to the change of the health index, and determining a lag moment; determining an effect coefficient in combination with the lagging time and the health index, determining the influence degree of the environmental data of each dimension on the batching effect, and screening to obtain an influence environmental dimension in combination with the influence degree of the environmental data of all dimensions on the batching effect and the predicted value of the environmental data in the next batch; and adjusting the ratio of additives in the same feeding stage in the next batch based on the influence environment dimension. According to the method, the ingredient adjustment logic can be optimized, and the adaptability of ingredients in a multi-dimensional complex scene is improved.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention discloses an application of a BnaMYB7 gene or an encoded protein thereof in regulating and controlling the oil content of rape, and belongs to the fields of genetic engineering and crop genetic breeding. The nucleotide sequence of the BnaMYB7 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, an agrobacterium-mediated genetic transformation method is used, an overexpression vector of the BnaMYB7 gene and a CRISPR / Cas9 gene editing vector are respectively transformed into a genome of the rape, the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is obtained, and the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is determined by measuring the oil content of seeds of overexpression and mutant materials. It is found that the BnaMYB7 gene positively regulates the oil content of the rape, so that rape varieties with different oil contents can be obtained by using the gene to perform molecular breeding on the rape. The feed additive is of great significance in relieving grain and oil tension and guaranteeing feed safety.

The invention relates to a betaine phosphate-containing feed additive for improving net energy level and a preparation method and application thereof, and belongs to the technical field of feed additives, the preparation method comprises the following steps: preparing composite betaine phosphate, preparing composite zinc methionine, and mixing; the preparation of the composite betaine phosphate comprises the following steps: preparing a betaine phosphate crude product, preparing a composite silicon dioxide carrier, and crystallizing; the preparation of the compound zinc methionine comprises the following steps: preparing an activated silicon dioxide carrier, adsorbing starch and adsorbing the zinc methionine; in the step of mixing, the compound betaine phosphate and the compound zinc methionine are mixed and then stirred at room temperature, and the betaine phosphate-containing feed additive capable of improving the net energy level is obtained. The feed additive prepared by the invention has a strong effect of improving the net energy level of broiler chickens, is not easy to have the problems of bonding and clustering, and is good in storage resistance.

The invention belongs to the technical field of microorganisms, and discloses a high-temperature-resistant vibrio alginolyticus bacteriophage and a composition and application thereof, the high-temperature-resistant vibrio alginolyticus bacteriophage is named as vibrio alginolyticus bacteriophage PJ644 and is preserved in China General Microbiological Culture Collection Center on August 16, 2024, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9624. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. The bacteriophage has excellent high temperature resistance, and the bacteriophage and the bacteriophage composition not only can effectively prevent and treat various vibrio infections, but also can be used as active ingredients for preparing bacteriophage pharmaceutical preparations, aquatic feed additives, water disinfectants and aquatic product preservatives, and are used for effectively preventing and treating diseases caused by vibrio infections in aquaculture farms. The use is safe, and the problems of antibiotic residues and pathogen drug resistance caused by use of antibiotics are effectively avoided.

This invention relates to the field of biocatalysis technology, and more particularly to an aflatoxin oxidase, its preparation method, and its applications. This invention utilizes a self-designed artificial intelligence enzyme design workflow to de novo design a novel aflatoxin oxidase, DnD-AFO. Compared to the traditional wild-type AFO, DnD-AFO exhibits enhanced activity, better thermal stability (its activity is significantly superior to the wild-type after high-temperature granulation), and improved resistance to pepsin and acid. Furthermore, it further enhances the detoxification efficiency against aflatoxin, making it well-suited as a food or feed additive for applications in agriculture and food engineering, among other related industries, demonstrating significant commercial and added value.

The invention relates to a novel camellia saponin derivative feed additive and a preparation method thereof, and belongs to the technical field of feed additives. The modified chitosan is used in the flocculation and impurity removal process for preparing the camellia oleifera saponin derivative, and the modified chitosan reduces the loss of effective components caused by flocculation and impurity removal while ensuring the purity of the camellia oleifera saponin derivative; soybean lecithin and gelatin are used as capsule walls, the camellia saponin derivative and pyropheophorbide-a are wrapped, pyropheophorbide-a can generate singlet oxygen under excitation of visible light, phospholipid bonds are damaged, the camellia saponin derivative is released, and therefore the slow release effect is achieved; the novel camellia saponin derivative feed additive prepared by the invention has good antibacterial property and timeliness.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum with high yield of nano-selenium and application of the bifidobacterium longum in production of nano-selenium, the bifidobacterium longum with high yield of nano-selenium can convert inorganic selenium salt into nano-selenium, and the content of nano-selenium enriched in freeze-dried bacterial powder is up to 24000mg / kg. The selenium-rich feed additive can be directly used for selenium dietary supplements or selenium-rich animal product production, and has potential application prospects in the fields of selenium-rich food and feed additives.

The invention relates to a composition for treating large yellow croaker pyramidoniasis as well as a preparation method and application thereof, and belongs to the technical field of aquaculture. The composition comprises the following components: curcumin, bile acid, beta glucan, bacillus subtilis freeze-dried powder, marine red yeast freeze-dried powder, vitamin C, vitamin E and ethylicin. When the feed additive is added into basal feed to feed large yellow croakers infected with the trypanosomiasis, the death rate of sick fishes can be effectively reduced, the polypide loading capacity is remarkably reduced, and pathological injuries of livers and spleens of the large yellow croakers are relieved. In addition, the composition can also improve the non-specific immunity of the fish body, improve the liver function, enhance the antioxidant capacity of the liver, and improve the lipase activity of the intestinal tract at the same time. Therefore, the composition can be used as a medicine or a feed additive for treating the large yellow croaker pyramidoniasis, has the advantages of small dosage and remarkable effect, and provides a new technical approach for solving the problem that the current large yellow croaker pyramidoniasis lacks an effective prevention and control means.

The invention relates to the technical field of probiotics, in particular to lactobacillus salivarius and application of the lactobacillus salivarius in regulating flora balance of digestive tracts of mutton sheep. The invention provides a lactobacillus salivarius KS1018, and the preservation number of the lactobacillus salivarius KS1018 is CCTCC (China Center for Type Culture Collection) NO: M 2025020. The lactobacillus salivarius KS1018 is a strain which is separated from dolang sheep rumen fluid and has the characteristics of high temperature resistance, acid resistance and cholate resistance, and has the advantages of low immunogenicity and high safety when being applied to mutton sheep. Due to the high-temperature-resistant characteristic, high stability can be kept in the feed mixing and processing process, and the application effect is further improved. The acid-resistant and cholate-resistant characteristics can reduce the influence of the digestive tract on the activity of the feed additive, so that the feed additive effectively plays a role in the digestive tract of the ruminant. The strain is good in palatability after being fed, and can effectively regulate digestive tract flora of ruminants, restore digestive tract flora balance and improve the disease-resistant potential of the ruminants.

The invention discloses a holothurian culture feed additive and a preparation method thereof, and belongs to the technical field of aquaculture. According to the technical scheme, the holothurian culture feed additive comprises the following components in percentage by mass: 45%-55% of fish meal, 2%-3% of butyric acid bacteria, 25%-30% of compound probiotics, 2%-4% of a Chinese herbal medicine extract, 8%-12% of a fermentation substrate, 10%-15% of seaweed meal, 0.5%-1% of vitamin E, 0.5%-1% of selenium yeast and the balance of a carrier, the compound probiotics are composed of lactic acid bacteria, bacillus and photosynthetic bacteria according to the mass ratio of 2: 1: 1; the Chinese herbal medicine extract is a mixture of astragalus polysaccharide and an asarum extract in a mass ratio of 3: 1; through the synergistic effect of the fish meal, the compound probiotics, the Chinese herbal medicine extract and other components, nutrition balance and intestinal flora regulation and control are achieved, the problems of slow growth, intestinal diseases and water quality pollution caused by traditional feed are effectively solved, and the sea cucumber feed has the advantages that the survival rate of sea cucumbers is increased, and the culture benefits are increased.

The invention provides a chitosan composite material and a compound feed additive, and relates to the technical field of feed additives. The chitosan composite material comprises cross-linked chitosan and a porous carrier, the cross-linked chitosan is coated on the surface of the porous carrier; the porous carrier comprises a first porous carrier and a second porous carrier, the average particle size of the first porous carrier is 80-150 [mu] m, and the average particle size of the second porous carrier is 10-30 [mu] m. The chitosan composite material disclosed by the invention has relatively good heavy metal ion adsorption performance and can be used as a feed additive.

The invention provides an anti-white spot syndrome virus peptide derived from litopenaeus vannamei, and belongs to the technical field of biology. The amino acid sequence of the anti-white spot syndrome virus peptide provided by the invention is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO.2. The invention further provides a recombinant expression vector and host bacteria containing the anti-white spot syndrome virus peptide gene, and application of the recombinant expression vector and host bacteria in anti-white spot syndrome virus drugs, vaccines, feeds and feed additives, and the recombinant expression vector and host bacteria have wide application prospects and economic benefits.

The invention belongs to the technical field of biology, and discloses application of wogonin in resisting siniperca chuatsi and frog iridovirus, the wogonin can effectively inhibit over-generation of ROS caused by MRV virus infection, and oxidative stress damage of host cells is relieved in a dose-dependent mode. The wogonin can be used for preventing and treating the siniperca chuatsi and frog iridovirus, the high expression state of a proinflammatory gene can be reduced, intestinal tissue damage induced by MRV infection can be relieved, proliferation of MRV in the micropterus salmoides is effectively inhibited, the MRV challenge survival rate of the micropterus salmoides is increased, a basis is provided for preparing a product for resisting the siniperca chuatsi and frog iridovirus from wogonin, and the wogonin has important application value. Meanwhile, the invention also discloses a medicine, a fish feed and a fish feed additive of the wogonin.

The invention belongs to the technical field of bacteriophages, and discloses an edwardsiella bacteriophage, a bacteriophage composition and application thereof, the bacteriophage is named as edwardsiella bacteriophage PREE 50, the bacteriophage is preserved in China General Microbiological Culture Collection Center on May 26, 2025, and the preservation number of the bacteriophage is CGMCC NO. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.46488. The bacteriophage PREE 50 is wide in lysis spectrum, high in lysis rate for various types of edwardsiella, good in acid-base stability and temperature stability and capable of being used for preparing drugs, water disinfectants, aquatic feed additives, detection kits and the like for treating or preventing edwardsiella infection, and the bacteriophage PREE 50 can be applied to preparation of drugs, water disinfectants, aquatic feed additives, detection kits and the like while solving edwardsiella infection. And the problems of antibiotic residues and pathogen drug resistance caused by the use of antibiotics are avoided.

The invention belongs to the technical field of feed additives, and particularly relates to a feed additive for improving the production performance, egg quality and body health of laying hens and application of the feed additive. The feed additive is composed of mulberry leaf powder and bacillus licheniformis. The invention also provides a feed which is composed of a basic feed and the feed additive, each 1kg of the feed contains 1 * 10 < 10 > CFU of bacillus licheniformis, and the mass of the mulberry leaf powder is 9%-15% of the mass of the feed. Animal experiments prove that the feed additive disclosed by the invention has no obvious negative influence on the production performance and the body health of laying hens, and can also obviously improve the egg quality. The invention provides a feasible scheme for reduction and substitution of soybean meal and corn in livestock and poultry feed, and has important practical significance for relieving structural shortage of protein feed raw materials.

The invention relates to the technical field of microorganisms, and particularly discloses a lactobacillus paracasei KQNIU, a microbial preparation and application of the lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, the preservation number is CGMCC No.35739, and the Lactobacillus paracasei KQNIU is classified and named as Lactobacillus paracasei, and the Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The lactobacillus paracasei KQNIU provided by the invention has good gastrointestinal tract tolerance, has an inhibition effect on pathogenic bacteria such as escherichia coli, salmonella and the like, can be prepared into a microbial preparation or a feed additive for preventing and treating calf diarrhea, replaces antibiotics and realizes green and healthy breeding.

The invention relates to the technical field of microorganisms, and particularly discloses a metagen as well as a preparation method and anti-inflammatory application thereof. The method for preparing the metagen comprises the following steps: (1) carrying out solid-state fermentation by taking bacillus amyloliquefaciens CAU X1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.26769 and bacillus coagulans B2 with the preservation number of CGMCC No.26767 as zymophyte together, so as to obtain a fermentation product, wherein the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together, and the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together; (2) extracting the fermentation product by using a water-soluble solvent, centrifuging to take supernatant, and freeze-drying to obtain powder; or extracting the fermentation product by using an organic solvent, centrifuging and taking the supernatant, and volatilizing the organic solvent to obtain the extract. The metagen provided by the invention has anti-inflammatory activity, can improve bacterial enteritis, can play roles in resisting inflammation, regulating body immunity, optimizing intestinal flora structure and improving bacterial enteritis, can be used for medicines or feed additives, and has a good application prospect.

The invention relates to a method for inhibiting rumen gas production by using a genetically modified laver filament as a ruminant feed additive and a method for inhibiting methane production by using the genetically modified laver filament as the ruminant feed additive. A porphyra yezoensis bromine peroxidase gene is cloned, an overexpression vector of the gene is constructed, porphyra yezoensis thallus is used as a receptor for genetic transformation, and a porphyra algal strain of which the key enzyme expression quantity is remarkably improved is obtained through screening. The method comprises the following steps: inducing thalli to generate protonema, crushing the protonema by using a crusher, carrying out ventilated large-scale culture in a seawater culture medium added with PES by using a column bioreactor to obtain a large number of protonema materials, collecting the protonema materials, crushing the protonema materials by using the crusher, and drying at low temperature. The obtained laver protonema powder is put into a vacuum bag for low-temperature preservation in dark light, and can be applied to preparation of ruminant feed as a dairy cow feed additive. The filamentous can accumulate halogenated compounds such as bromidogen and the like, can inhibit the activity of a key enzyme generated by methane in rumen, and further inhibits the generation of methane.

The invention belongs to the technical field of biological information calculation, and discloses a pet feed additive formula calculation model optimization system, which comprises a data interface for acquiring raw material parameters and data containing environmental stress factors; the memory stores a heterogeneous neural network model, and comprises a first time sequence convolution subnet, a second cascade processing subnet and a global gating distribution unit which are arranged in parallel and are coupled through a lateral suppression channel; the processor operates the model, the global gating distribution unit is used for dynamically adjusting the computing power weight between the subnets according to the system energy state tensor, and back propagation iteration is carried out based on a nonlinear constraint penalty term. The problems of convergence difficulty and negative migration when a general model processes high-dimensional strong-coupling biological data are solved, and the prediction precision and safety of formula optimization are improved.

The invention belongs to the technical field of preparation of feed additives, and particularly relates to a method for preparing seaweed-based polysaccharide for feed based on composite low-value brown algae. According to the method, multiple kinds of composite low-value brown algae are adopted as raw materials for the first time, composite alkali liquor is creatively heated to assist ultrasonic extraction to prepare the seaweed-based polysaccharide for the feed, and in some embodiments of the method, compared with an ultrasonic extraction method without alkali heating, the polysaccharide extraction rate is increased by 1.2 times through composite alkali liquor heating to assist ultrasonic extraction. The used raw material is low-value brown algae which is wide in source and low in cost; the preparation process is simple to operate, clear in parameters of each step and easy to realize industrial production. The prepared seaweed-based polysaccharide for the feed is convenient to add in the feed, can remarkably improve the growth performance of animals, enhance the immunity and improve the intestinal health, has good economic benefits and social benefits, has wide application prospects in the feed industry, and is high in practicability.

A peptidoglycan hydrolase LysPH1 having a broad-spectrum lytic activity, and a mutant and the use thereof, which belong to the technical field of biological formulations. An antibacterial peptide LysPH1-CP, a peptidoglycan hydrolase LysPH1 containing the antibacterial peptide LysPH1-CP and a peptidoglycan hydrolase mutant LysPH1-K containing the peptidoglycan hydrolase LysPH1 all have good thermal stability, and can broadly lyse both Gram-negative bacteria and Gram-positive bacteria. Moreover, the peptidoglycan hydrolase LysPH1 and the peptidoglycan hydrolase mutant LysPH1-K can be solubly expressed in Escherichia coli, and have high enzymatic activity. Therefore, the antibacterial peptide LysPH1-CP, the peptidoglycan hydrolase LysPH1 and the peptidoglycan hydrolase mutant LysPH1-K have good application prospects in the preparation of an anti-infective drug and the research and development of feed additives.

The invention provides a compound feed additive capable of improving the flavor of eggs, a preparation technology and a compound feed, and relates to the technical field of feed preparation.Main components in shaddock peel are analyzed, the main components influencing the flavor of the eggs are screened out, and chemical components and shaddock peel powder are adopted to prepare the compound feed additive. The grapefruit flavor extract (active ingredients such as limonene, polyphenols and the like) prepared on the basis of the grapefruit peel powder. The preparation method comprises the following steps: compounding the raw materials in parts by weight: 20-40 parts of linalool, 5-10 parts of linalyl acetate, 2-4 parts of limonene, 1-2 parts of vanillyl alcohol, 0.7-1.4 parts of geranyl acetate, 5-10 parts of myrcene, 80 parts of pomelo peel powder, 150 parts of a carrier and 70 parts of a pomelo flavor extract; the total addition amount of the shaddock peel powder in the basal feed is reduced from 2% to 0.1%, a good flavor improvement effect can be maintained, and dependence on shaddock peel raw materials is greatly reduced.

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

The invention relates to the technical field of probiotic development and utilization, in particular to a goose source Weissella cibaria F11 and application thereof. The Weissella cibarius F11 is preserved in the China General Microbiological Culture Collection Center on July 11, 2025, and the preservation number is CGMCC NO.35211. The Weissella cibarius F11 has the characteristics of good acid resistance and cholate resistance, and can be used for preparing the acid-resistant and cholate-resistant strain. And the bacillus subtilis has a good inhibition effect on pathogenic bacteria such as escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and salmonella typhimurium, and also has stable acid production capacity and good safety. The Weissella sinus F11 provided by the invention meets the probiotic characteristic standard, the antibiotic resistance spectrum of the Weissella sinus F11 meets the feed probiotic safety standard, the growth of goslings can be remarkably promoted, the death rate and diarrhea occurrence rate of the goslings can be remarkably reduced, and the weissella sinus F11 can be used for preparing feed probiotics. The development potential in the fields of preparation of bacteriostatic and antibacterial drugs, probiotics for poultry feed, feed additives, poultry feed and the like is huge.

The invention discloses a strain of lactobacillus plantarum SMU-KDLP2312 and application thereof, the strain of lactobacillus plantarum SMU-KDLP2312 is classified and named as Lactobacillus plantarum, the strain of lactobacillus plantarum SMU-KDLP2312 is named as Lactobacillus plantarum, and the strain of lactobacillus plantarum SMU-KDLP2312 is named as Lactobacillus plantarum. The strain is preserved in Guangdong Microbial Culture Collection Center on March 21, 2025, with the preservation number of GDMCC No.66040. The strain is preserved in the Guangdong Microbial Culture Collection Center. The lactobacillus plantarum SMU-KDLP2312 system is obtained by being separated and screened from intestinal tracts of Tibetan sheep, and has relatively strong stress resistance and in-vitro antibacterial ability. Meanwhile, the lactobacillus plantarum SMU-KDLP2312 has a relatively high survival rate in an acid environment, has relatively good tolerance to bile salt, has the effect of reducing cholesterol, and has the potential of becoming a good probiotic effect regulator. As one of feed additive strains, when applied to daily ration, the strain can promote the growth performance of Tibetan sheep to a certain extent, adjust the balance of animal intestinal flora and enhance the proliferation of beneficial bacteria.

The invention provides application of bacillus tequilensis Bt-CO as a feed additive in largemouth bass culture, and belongs to the technical field of largemouth bass culture. The preservation number of the bacillus tequilensis Bt-CO is GDMCC No: 64960, the influence of the Bt-CO on the growth, survival, physical signs, immunity, antioxidant reaction, digestive enzyme, disease resistance, intestinal microbiota and metabolism of the largemouth bass is evaluated, and the probiotic potential of the Bt-CO and the effect of the Bt-CO in enhancing the resistance of the largemouth bass to aeromonas veronii infection are proved; bt-CO can be developed into a substitute of antibiotics, and a theoretical framework and a practical basis are provided for reasonable application of probiotics in aquaculture.