1995 results about "Feed additive" patented technology

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention relates to the technical field of microorganisms, in particular to application of streptococcus non-lactolyticus GX-7 and a complex microbial inoculant thereof in sugarcane leaf silage, the strain GX-7 is separated from healthy pig manure by a research group, and through detection, the strain has good cholate resistance and is suitable for surviving in intestinal tracts, and the strain GX-7 has good cholate resistance and is suitable for being used as a feed additive. The antibacterial effect on pseudomonas fluorescens, listeria monocytogenes, salmonella choleraesuis and shigella flexneri is achieved; the strain has a good fermentation capability on sugarcane leaves, and the sugarcane leaves are inoculated with a complex microbial inoculant prepared from streptococcus non-lactolyticus GX-7 and streptococcus non-lactolyticus GX-2, so that a synergistic fermentation effect can be achieved; after the silage prepared from the complex microbial inoculant is fed to female buffalo in the gestation period, it is found that the silage can effectively increase the content of milk protein, milk total solids and milk non-fat solids in colostrum of the female buffalo and relieve diarrhea of the female buffalo, and the silage is safe and good.

The invention discloses a microbial compound feed additive, which is prepared from the following ingredients in parts by weight: 35 to 45 parts of traditional Chinese medicine residues, 25 to 35 parts of soybean meal, 15 to 25 parts of bran, 8 to 12 parts of corn flour, 0.3 to 0.7 part of selenium-enriched yeast, 8 to 12 parts of aspergillus niger spore powder, 10 to 15 parts of bacillus subtilis, 6 to 9 parts of lactobacillus plantarum and 5 to 8 parts of candida utilis. The invention relates to the technical field of feed additives, through the synergistic effect of the aspergillus niger, the bacillus subtilis, the lactobacillus plantarum and the candida utilis, the aspergillus niger can generate various enzymes, and decomposition of raw materials and release of nutritional ingredients are facilitated; the bacillus subtilis can enhance the immunity of livestock and poultry and inhibit the growth of harmful bacteria; lactobacillus plantarum can regulate intestinal microecological balance and promote digestion and absorption; candida utilis is rich in protein and vitamins and provides nutrition for livestock and poultry, the four components cooperate with each other, the function limitation of a single strain is broken through, and the health level and production performance of livestock and poultry are comprehensively improved.

The invention discloses a feed additive ratio control method and system based on environmental perception. The method comprises the steps that breeding environment, feed characteristics, equipment operation and animal physiological parameters are collected, and a high-quality parameter set is generated after preprocessing; calculating an environment stress index based on a multi-factor data fusion algorithm and analyzing a change trend; combining historical and current data, using time sequence prediction and a machine learning algorithm to predict additive requirements and generating an optimal proportioning strategy; a control instruction is generated according to the strategy, and the additive conveying and spraying process is regulated and controlled; and a matching strategy and control parameters are corrected in real time through closed-loop feedback, so that accurate regulation and control are realized. The device can dynamically adapt to environmental changes, reduce animal stress, and improve mixing uniformity and nutrition retention effect.

Provided herein, inter alia, are ruminant feed or feed additive compositions, and methods of using these feed or feed additive compositions to reduce methane emissions in ruminants while improving animal performance, these feed or feed additive compositions comprise one or more compounds that increase hydrogen production in ruminants, such as, but not limited to, 3-nitroxypropanol (3NOP) and one or more acetogens.

The invention discloses a feed additive for relieving animal mycotoxin poisoning and a preparation method thereof. The feed additive comprises the following components in parts by weight: 25-30 parts of attapulgite, 25-30 parts of montmorillonite, 25-30 parts of yeast cell walls, 2-5 parts of vitamin E, 5-10 parts of taurine and 5-10 parts of lipoic acid. The feed additive disclosed by the invention can be used for remarkably improving the harm problem of mycotoxin in the livestock and poultry breeding production process and reducing the economic loss of livestock and poultry farmers caused by the mycotoxin pollution of the feed.

The invention relates to the technical field of compound fertilizers, and discloses a boron-zinc-molybdenum synergistic compound fertilizer, which is prepared from the following raw materials in parts by weight: calcium nitrate, ammonium polyphosphate, potassium sulfate, magnesium sulfate, disodium octoborate tetrahydrate, sugar alcohol chelated zinc, sodium molybdate and a synergistic auxiliary agent, the feed additive is prepared from a feed additive, an activator, 5-alginic acid oligosaccharide, aminolevulinic acid, sugar alcohol chelated magnesium, an antagonist, a stress-resistant enhancer and a coating material. According to the boron-zinc-molybdenum synergistic interaction compound fertilizer and the preparation process thereof, the disodium octoborate tetrahydrate, the sugar alcohol chelated zinc and the sodium molybdate are compounded according to a specific proportion, antagonism is avoided through proportion limitation, the synergistic effect of the three elements is remarkably enhanced, and the problem that a single element is insufficient or excessive is solved; chelated elements such as sugar alcohol chelated zinc and sugar alcohol chelated magnesium are combined with an activating agent (humic acid chelated calcium and silica sol) to form a gel-state chelate, so that the trace elements can be prevented from being fixed by soil, and the transfer efficiency of the trace elements in crops is improved.

The invention relates to the technical field of feed formulas, and particularly discloses a natural mixed feed additive and a preparation method thereof. The natural mixed feed additive comprises the following raw materials in parts by mass: 4-7 parts of vitamin E, 5-15 parts of beta-carotene, 10-16 parts of soybean oil, 20-35 parts of deionized water, 5-8 parts of modified probiotic microcapsules and 1-5 parts of an emulsifier, wherein the modified probiotic microcapsules contain sodium alginate-pectin composite microencapsulated probiotics; the preparation method comprises the following steps: heating 10-16 parts of soybean oil to 35-45 DEG C, then adding 4-7 parts of vitamin E and 5-15 parts of beta-carotene, and stirring; adding 1-5 parts of an emulsifier into 20-35 parts of deionized water, heating to 45-55 DEG C, stirring, and then slowly adding into the mixed solution obtained in the step S1; 5-8 parts of modified probiotic microcapsules are added, and stirring is conducted for 8-12 min under the condition of 100-200 r / min. The natural mixed feed additive disclosed by the invention has the advantage that the defect that the feed additive still has defects in the aspect of reducing intestinal diseases of animals can be overcome.

The invention relates to the technical field of feed ingredient optimization, in particular to an ingredient optimization and adjustment system for a phage type antibiotic-free feed additive. Comprising the following steps: acquiring multi-dimensional environmental data and antibody content indexes and stress state information of individuals; determining a health index according to the antibody content index and the stress state information; performing batching delay analysis according to the change of the health index, and determining a lag moment; determining an effect coefficient in combination with the lagging time and the health index, determining the influence degree of the environmental data of each dimension on the batching effect, and screening to obtain an influence environmental dimension in combination with the influence degree of the environmental data of all dimensions on the batching effect and the predicted value of the environmental data in the next batch; and adjusting the ratio of additives in the same feeding stage in the next batch based on the influence environment dimension. According to the method, the ingredient adjustment logic can be optimized, and the adaptability of ingredients in a multi-dimensional complex scene is improved.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention discloses an application of a BnaMYB7 gene or an encoded protein thereof in regulating and controlling the oil content of rape, and belongs to the fields of genetic engineering and crop genetic breeding. The nucleotide sequence of the BnaMYB7 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, an agrobacterium-mediated genetic transformation method is used, an overexpression vector of the BnaMYB7 gene and a CRISPR / Cas9 gene editing vector are respectively transformed into a genome of the rape, the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is obtained, and the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is determined by measuring the oil content of seeds of overexpression and mutant materials. It is found that the BnaMYB7 gene positively regulates the oil content of the rape, so that rape varieties with different oil contents can be obtained by using the gene to perform molecular breeding on the rape. The feed additive is of great significance in relieving grain and oil tension and guaranteeing feed safety.

The invention relates to a betaine phosphate-containing feed additive for improving net energy level and a preparation method and application thereof, and belongs to the technical field of feed additives, the preparation method comprises the following steps: preparing composite betaine phosphate, preparing composite zinc methionine, and mixing; the preparation of the composite betaine phosphate comprises the following steps: preparing a betaine phosphate crude product, preparing a composite silicon dioxide carrier, and crystallizing; the preparation of the compound zinc methionine comprises the following steps: preparing an activated silicon dioxide carrier, adsorbing starch and adsorbing the zinc methionine; in the step of mixing, the compound betaine phosphate and the compound zinc methionine are mixed and then stirred at room temperature, and the betaine phosphate-containing feed additive capable of improving the net energy level is obtained. The feed additive prepared by the invention has a strong effect of improving the net energy level of broiler chickens, is not easy to have the problems of bonding and clustering, and is good in storage resistance.

The invention relates to the technical field of fermentation equipment, in particular to high-safety feed additive fermentation equipment. Comprising a bottom plate; the supporting seat is connected to the top of the bottom plate; the supporting frame is connected to the top of the bottom plate; the rotating blocks are rotationally connected to the supporting seat and the supporting frame respectively; the connecting frame is connected to the rotating block, and placing grooves are formed in the inner bottom of the connecting frame in the circumferential direction at intervals; the fermentation cylinder is placed in the placement groove, and a through hole is formed in the top of the fermentation cylinder; the first driving motor is mounted at the top of the bottom plate; and the universal coupling is mounted between the output shaft of the first driving motor and one of the rotating blocks. The rotatable connecting frame is matched with the detachable fermentation cylinder, a traditional stirring mechanism is replaced, material mixing is achieved through overall rotation, the problem that materials remain on the stirring shaft and the blades can be effectively solved, and meanwhile pollution-free and safety in the fermentation process can be guaranteed through cooperation of the pressing plate fixing structure and the sealing assembly.

The invention relates to a preparation method of traditional Chinese medicine residue metabiotics and application of the traditional Chinese medicine residue metabiotics in fish feed, and belongs to the technical field of feed additives. Beneficial bacteria such as bacillus subtilis, lactobacillus lactis, bifidobacterium longum and saccharomyces cerevisiae are separated from intestinal contents of grass carp and are subjected to enlarged culture, then traditional Chinese medicine residues such as fresh ginger, folium mori, ginseng, fructus lycii, rhizoma curcumae longae, liquorice and mint are added, staged mixed fermentation culture is performed, obtained metabolites are added into fish feed, and the fish feed is obtained. The feeding rate and the growth rate of fishes such as mandarin fish and micropterus salmoides are effectively improved, and the resistance is enhanced.

The invention discloses clostridium butyricum as well as a culture method and application thereof, belongs to the field of feed additives, and aims to solve the problems that a controllable fattening period is shortened and the efficacy of the clostridium butyricum cannot be completely exerted due to the fact that a wide range of clostridium butyricum needs a relatively long adaptation period for an endogenous intestinal environment of an animal. The clostridium butyricum from the North Naa Biotechnology Co., Ltd. Is cultured by simulating an animal endogenous environment in vitro, and after the clostridium butyricum which completely adapts to animal endogenous is obtained, the screened animal endogenous clostridium butyricum is coated by adopting a coating technology, so that the clostridium butyricum is not damaged by a strong acid environment in an animal stomach bag; the clostridium butyricum strain has the advantages that the clostridium butyricum strain can be used for preparing animal endogenous clostridium butyricum, a large quantity of animal endogenous clostridium butyricum can reach intestinal tracts and be released, and the animal endogenous clostridium butyricum can quickly adapt to animal intestinal environment due to higher adaptability of the strains, and can quickly form efficient colonization and functional expression under the condition of almost no adaptation period. The feed additive can be used as a biological feed additive.

The invention discloses a composite microbial preparation for treating obesity of pet dogs and application of the composite microbial preparation, and belongs to the technical field of microbial preparations. The compound microbial preparation is prepared by fermenting lactobacillus acidophilus L100 and lactobacillus reuteri GBW1146, and the preservation number of the lactobacillus reuteri GBW1146 is CGMCC (China General Microbiological Culture Collection Center) No. 34455. The compound microbial preparation is prepared by fermenting lactobacillus acidophilus L100 and lactobacillus reuteri GBW1146. The compound microbial preparation can remarkably reduce digestion and absorption of intestinal tracts of dogs on fat and reduce triglyceride and cholesterol levels in blood, so that the average food intake and average calorie consumption of the dogs are reduced, and finally the effect of reducing obesity of the pets is achieved. The compound microbial preparation provided by the invention enhances the competitiveness of the pet feed additive and series products thereof in China in the international market, and has a wide application prospect.

The invention belongs to the technical field of microorganisms, and discloses a high-temperature-resistant vibrio alginolyticus bacteriophage and a composition and application thereof, the high-temperature-resistant vibrio alginolyticus bacteriophage is named as vibrio alginolyticus bacteriophage PJ644 and is preserved in China General Microbiological Culture Collection Center on August 16, 2024, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9624. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. The bacteriophage has excellent high temperature resistance, and the bacteriophage and the bacteriophage composition not only can effectively prevent and treat various vibrio infections, but also can be used as active ingredients for preparing bacteriophage pharmaceutical preparations, aquatic feed additives, water disinfectants and aquatic product preservatives, and are used for effectively preventing and treating diseases caused by vibrio infections in aquaculture farms. The use is safe, and the problems of antibiotic residues and pathogen drug resistance caused by use of antibiotics are effectively avoided.

The invention belongs to the technical field of feed additives, and particularly relates to slow-release fat powder for pigs and a preparation method of the slow-release fat powder. The slow-release fat powder for pigs comprises an intragastric adhesion-slow release layer, a small intestine forepart bile acid response controlled release layer and a small intestine posterior microbe response controlled release layer. The prepared slow-release fat powder for the pigs is good in stability, accurate release of different nutritional ingredients in different parts of gastrointestinal tracts of the pigs can be achieved, and powerful support is provided for sustainable development of the pig raising industry.

The invention belongs to the technical field of probiotics, and particularly relates to fermented lactobacillus mucus BM01 with an effect of relaxing bowel and application of the fermented lactobacillus mucus BM01. A new fermented lactobacillus mucus BM01 strain is separated from excrement of a healthy infant in Guangzhou city, Guangdong province, a zebra fish constipation model is constructed through loperamide hydrochloride, and research finds that the strain can remarkably promote intestinal tract movement, increase the content of in-vivo serotonin and inhibit the enzyme activity of dipeptidyl peptidase-4, so that the constipation of the zebra fish can be inhibited, the constipation of the zebra fish can be inhibited, and the constipation of the zebra fish can be inhibited. Therefore, the bowel relaxing effect is achieved. It is indicated that the BM01 strain can play the bowel relaxing effect through multiple mechanisms, has significant application value, can be used for research and development of drugs for treating constipation, irritable bowel syndrome and other intestinal diseases or development of health care products with the bowel relaxing effect, and can also be used as a feed additive to improve animal intestinal health and reduce disease risks. The culture benefit is improved.

The invention discloses a traditional Chinese medicine composition for improving mutton quality and a preparation method thereof, and belongs to the technical field of feed additives. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 20 to 30 parts of radix angelicae, 10 to 15 parts of radix astragali seu hedysari, 10 to 15 parts of fructus crataegi and 10 to 15 parts of radix salviae miltiorrhizae. Experiments prove that by adding the traditional Chinese medicine composition provided by the invention into basic ration, the tenderness, meat color brightness, hardness, elasticity and other quality characteristics of mutton can be obviously improved; the content of unsaturated fatty acid in the mutton can be remarkably increased, and the purpose of improving the mutton quality is achieved. The invention provides a new choice for improving the mutton quality.

The invention discloses a preparation method of a mycotoxin degrading enzyme intelligent slow-release feed additive, and relates to the technical field of feed additive production, a double-emulsion embedding technology is adopted, enzyme is embedded in a multi-layer shell composed of chitosan, sodium alginate and an enteric polymer Eudragit S100, and then Ca < 2 + > ion cross-linking solidification is performed to obtain the mycotoxin degrading enzyme intelligent slow-release feed additive. And finally, freeze-drying or spray-drying to obtain the core-shell structure microcapsule. The obtained microcapsules are compounded with functional carriers such as yeast cell walls, sodium aluminosilicate, mannan oligosaccharide, talcum powder and the like to prepare finished products which can be directly added into various feeds. The release rate of the additive in simulated gastric juice with the pH value of 2.0 for 4 hours is lower than 15%, the release rate of the additive in simulated intestinal juice with the pH value of 6.8 for 6 hours exceeds 90%, the enzyme activity can be effectively protected, and targeted release of intestinal tracts is achieved; meanwhile, the compound carrier has a synergistic adsorption effect on various mycotoxins, and the degradation rates of aflatoxin B1, zearalenone and ochratoxin A in an in-vitro experiment respectively reach 87% or above.

The invention discloses an application of kaempferol in preparation of a medicine for treating or preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The kaempferol can obviously inhibit the replication of the PEDV in Vero and LLC-PK1 cells through 3C-like protease targeting the PEDV, so that the kaempferol can be used for preparing the medicine for preventing and treating the PEDV. Besides, kaempferol, as a natural substance separated from mulberries, can be used as a feed additive for a long time so as to achieve the purpose of preventing epidemic diseases, and does not cause generation of drug-resistant strains and other toxic and side effects after being used for a long time; the defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome.

The invention discloses a feed additive for improving animal immunity and a preparation method, and belongs to the technical field of animal feed. The nutrient components comprise the following raw materials in parts by weight: 10-20 parts of amino acids, 0.5-3.0 parts of vitamins and 15-22 parts of minerals; the traditional Chinese medicine components comprise 20-30 parts of radix astragali, 15-25 parts of radix codonopsis, 5-20 parts of rhizoma atractylodis macrocephalae, 10-20 parts of poria cocos, 10-15 parts of radix isatidis, 5-15 parts of honeysuckle flowers, 6-12 parts of echinacea purpurea, 3-12 parts of radix acanthopanacis senticosi and 3-8 parts of fiveleaf gynostemma herb; functional additives: 3-5 parts of probiotics, 2-4 parts of an enzyme preparation and 1-3 parts of an antioxidant. According to the invention, a multi-dimensional synergistic effect is achieved, and a traditional Chinese medicine immune enhancement-antiviral core formula system is formed; through cooperation with nutrients and functional additives, a composite protection system of immunity enhancement, nutrition optimization, intestinal health and stress resistance is constructed. The immunity and the disease resistance of animals are obviously improved.

The invention relates to rhodosporidium toruloides with high grease yield and application of the rhodosporidium toruloides. The rhodosporidium toruloides mutant strain with high grease content and high grease yield is obtained through a compound mutation method. According to the invention, a wild rhodosporidium toruloides strain RT is taken as a starting strain, a method of combining normal-pressure and room-temperature plasma mutagenesis with genome rearrangement is adopted, a stable genetic mutant strain RT-55 with the oil content and the yield obviously improved is obtained through oil content screening, and under the condition of a nitrogen-limited culture medium, the oil content of the mutant strain reaches 70.1% and is improved by 14.2%, and the yield of the mutant strain is greatly improved. The biomass is 4.11 g / L and is reduced by 0.8%, and the grease yield reaches 2.832 g / L and is improved by 10.9%. The rhodosporidium toruloides mutant strain provided by the invention can be used as a substitute of a grease raw material and is applied to the fields of aquatic feed additives and the like.

The invention belongs to the field of microorganisms and fermentation, and relates to a biological preparation, a preparation method thereof and application of the biological preparation in preparation of products for inhibiting escherichia coli. And the biological agent is a co-fermentation product of fructus forsythiae and bacillus amyloliquefaciens (Bacillus amyloliquefaciens). The biological preparation provided by the invention can obviously improve the secretion of IFN-alpha in a mouse body, reduce the production of IL-1beta, IL-6 and TNF-alpha, increase the proportion of CD3 + CD4 + and CD3 + CD8 + T cells, and improve the expression of ZO-1 and Occludin in duodenum and jejunum. Compared with single use of fructus forsythiae and bacillus amyloliquefaciens, the biological preparation shows a stronger synergistic effect, can effectively prevent and inhibit escherichia coli infection, and is not easy to generate drug resistance. The preparation method is simple, raw materials are easy to obtain, and the feed additive is suitable for developing antibacterial drugs and feed additives.

This invention relates to the field of biocatalysis technology, and more particularly to an aflatoxin oxidase, its preparation method, and its applications. This invention utilizes a self-designed artificial intelligence enzyme design workflow to de novo design a novel aflatoxin oxidase, DnD-AFO. Compared to the traditional wild-type AFO, DnD-AFO exhibits enhanced activity, better thermal stability (its activity is significantly superior to the wild-type after high-temperature granulation), and improved resistance to pepsin and acid. Furthermore, it further enhances the detoxification efficiency against aflatoxin, making it well-suited as a food or feed additive for applications in agriculture and food engineering, among other related industries, demonstrating significant commercial and added value.

The invention relates to a varied massinia, and in particular relates to a separated varied massinia P2-4 with broad-spectrum phagocytosis activity on pathogenic bacteria, and the varied massinia P2-4 is used for phagocytosis in a bacterium cracking manner, so that the activity of the varied massinia P2-4 is improved. The compound has phagocytosis activity on pathogenic bacteria such as pseudomonas aeruginosa, shewanella alginosa, aeromonas cainea, aeromonas hydrophila and photobacterium damsela; the Marsinia variabilis P2-4 provided by the invention can be applied to inhibition of pathogenic bacteria in an aquaculture water body; by adding the varied maleinia sp. P2-4 or the microbial inoculum thereof into the aquaculture feed, the immunity and / or the oxidation resistance and / or the disease infection resistance of the organism of aquaculture animals can be enhanced; the varied marssonia sp. P2-4 disclosed by the invention can be used as a feed additive for aquaculture or a biocontrol product for aquaculture, and can also be used for preventing and / or treating diseases of aquaculture animals.

The invention relates to an enzyme and bacterium combined feed additive for pre-digestion of feed raw materials as well as preparation and application of the enzyme and bacterium combined feed additive. The feed additive comprises: (a) an enzyme preparation, which is composed of protease, cellulase, xylanase, mannase, phytase, pectinase and keratinase, and has a total enzyme activity of 42500-45000 U / g; the probiotics are composed of bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae, and the viable count is larger than or equal to 107 CFU / g; and (c) the mass ratio of enzyme to bacteria is (1-2): 1. According to the additive, the enzyme preparation and the probiotics are jointly used, and the raw materials are effectively pretreated, so that the nutritional value and the digestibility of the feed are improved. The feed additive disclosed by the invention comprises a specific enzyme preparation and probiotic combination, and predigestion treatment on the feed raw materials is realized through an optimized preparation process and fermentation process, so that the nutritional value and the utilization value of the feed raw materials are improved. The additive is simple in preparation method, low in cost and environment-friendly, and has a wide application prospect.

The invention discloses an anti-lipopolysaccharide factor rALF-like protein and application thereof, and belongs to the technical field of molecular biology. The anti-lipopolysaccharide factor rALF-like protein provided by the invention belongs to secretory protein, the rALF-like protein can be competitively combined with an LPS structural domain of bacteria to achieve the purpose of inhibiting inflammation, and the expression of the coding gene Pv-ALF-like gene in blood cells, hepatopancreas, gill and intestinal tissues is remarkably up-regulated, so that the anti-lipopolysaccharide factor rALF-like protein can be used for inhibiting inflammation. The rALF-like protein disclosed by the invention has antibacterial and antiviral effects, lays a foundation for developing antibacterial and antiviral drugs and functional feed additives, and plays an important role in improving the resistance link of prawns.

The invention discloses a feed additive for improving mutton quality and producing low-mutton-smell mutton, which is characterized in that through combined application of phytosterol and dl-alpha-tocopheryl acetate, the microbial composition of the lamb intestinal tract is improved, especially the abundance of beneficial bacteria is improved, and the abundance of potential pathogenic bacteria is reduced. The combined use of the two can improve the intestinal microbial ecology, and significantly reduce the drip loss, shear force and Plt of mutton; 0.05, the mutton color and the tenderness of the mutton are improved; excrement causing secondary pollution to the environment is not generated. The application of the plant extract in the production of the low-mutton-smell mutton is reported for the first time, the mutton smell can be remarkably reduced by combining and adding the phytosterol and the dl-alpha-tocopheryl acetate according to a certain proportion, and meanwhile the mutton quality is improved. The feed additive disclosed by the invention is simple in components, remarkable in effect and simple and convenient to apply, and has great potential popularization and application values.