41 results about "Macrobrachium rosenbergii" patented technology

The invention relates to method of crossbreeding the exotic fresh water crayfish, especially to method of crossbreeding the Luo shi swamp shrimp. The invention introduces the distant Luo shi swamp shrimp sprout A, mating the female parent shrimp and male parent shrimp of the distant Luo shi swamp shrimp sprout A with that of native breed and getting the cross cut B1+ and negative cross B1-; mating the cross cut B1+ and negative cross B1- with the native breed and getting the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-; breeding the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-. The survival rate and seed-accumulating rate of the four kinks of sprout is all good, the composition of water, coarse ash content, coarse protein, coarse fat content, amino acid and aliphatic acid is higher than that of native obviously by applying the technique in this invention, especially the breed-accumulating rate of the cross B2-+ of B1- is increased to 67.25%, the rate of emergence to 94.56%.

The invention discloses a giant freshwater juvenile prawn cultivating method and belongs to the technical field of cultivation of giant freshwater juvenile prawns. Environmental conditions are optimized, a cultivating pond is rational constructed, shrimp seeds are scientifically released and breeding management is precisely performed; the method provides a set of complete method for improving survival rate during cultivation of juvenile prawns. The giant freshwater juvenile prawn cultivating method is simple to operate, low in cost and has remarkable effect, obvious economic benefits and social benefits.

The invention relates to macyobrachiun rosebergii larvae basal feed which comprises the components of imported fish meal, domestic fish meal, crude soybean meal, peanut meal, puffing soybean, macyobrachiun rosebergii shell meal, silkworm chrysalis, phospholipid oil, flour, squid paste, calcium dihydrogen, betaine, choline chloride, calcium powder, vitamin c lipid, taurine, inositol, deshell elements, allicin, multiple vitamins and composite mineral elements. The macyobrachiun rosebergii larvae basal feed has the advantages that the material cost is low, breeding cost is reduced effectively, the feed utilization amount is reduced by about 15%, beneficial elements of vitamins, mineral substances and the like are collocated scientifically, the nutrition is balanced and completely meets the nutrition and growth needs of macyobrachiun rosebergii larvae, the growth speed and the immunity of the macyobrachiun rosebergii larvae are increased, and the feed has no hormone or additives, and belongs to a natural green pollution-free product.

The invention relates to an ecological breeding method for mixed breeding of gold saddle rabbitfish and giant freshwater prawns. The ecological breeding method comprises the steps that 1, pond cleaning is performed before water inflowing, pond drying in the sun is performed for 14-16 days, and then water inflowing begins, wherein the salinity of a water body is 3-8; 2, the gold saddle rabbitfish and the giant freshwater prawns are bred in a breeding pond in a mixed mode, wherein the density of the gold saddle rabbitfish is 2500-2600 per mu, and the density of the giant freshwater prawns is 50000-5200p per mu; a seawater floating mixed feed and a giant freshwater prawn feed are fed respectively every day in month before the breeding process; only the seawater floating mixed feed is fed after one month of breeding. By adopting the breeding method, the gold saddle rabbitfish survival rate can be up to 85%, the yield of the gold saddle rabbitfish per mu is up to 192 kg, the yield of the giant freshwater prawn per mu is up to 200 kg, bred giant freshwater prawns are large and few get ill, a breeding effect is remarkable, and the ecological breeding method has a good application prospect.

The invention discloses a giant freshwater prawn shrimp seed period compound feed and a preparation method thereof. The giant freshwater prawn shrimp seed period compound feed consists of the following raw material ingredients in percentage by weight: 15-20% of Manhattan fish meal, 18-25% of bean pulp, 10-14% of peanut cake, 4-7% of corn protein powder, 20-25% of high gluten flour, 1-3% of molasses yeast, 1-3% of squid paste, 0-1% of salt, 0-0.5% of monocalcium phosphate, 1-2% of fish oil, 1-2% of soyabean lecithin, 1% of vitamin premix and 1% of mineral substance premix. The compound feed disclosed by the invention has the advantages of simple proportion and balanced nutrition, the survival rate of the giant freshwater prawn shrimp seed period can be obviously improved, and the growth performance of the giant freshwater prawn can be improved.

Provided is a shrimp-vegetable-fish three-dimensional ecological culture method. The method comprises: firstly, using quick lime to clean a pond and disinfect; exposing a culture pond in the sun for one week, after water injection, putting shrimp fries in the pond, 20-30 days after putting the shrimp fries, putting grass carp, silver carp, spotted silver carp, carp, and giant freshwater prawns inthe pond; feeding South America white shrimp fodder, through observation, determining feeding amount of the fodder, not feeding fodder of other culture varieties; through a floating bed, planting water spinach on a water surface, since prawns are cultured in the pond, pond water is eutrophic, using root systems of the water spinach to absorb eutrophic substances in the pond water, applying fertilizer for the pond being not needed. Three-dimensional ecological efficiency among planted and cultured creatures is used, ecological three-dimensional effect among animals, plants, and microorganisms is used, and heterosis (antagonism) effect is used. A water surface space is fully used, nutrient substances in the water are reasonably used, incomplete decomposition of organic materials in the pondis reduced, occurrence of diseases is reduced, and comprehensive benefits of pond culture is improved. The method has little investment, and is simple in maintenance, and low in manufacturing and operation cost.

The invention discloses a genetic diversity analysis method for a inbreeding male giant freshwater prawn, which comprises the following steps of: step 1, acquiring inbreeding giant freshwater prawn samples with different traits, and extracting the DNA; step 2, selecting a microsatellite marker combination and designing a specific PCR primer set for amplification; step 3, evaluating the number of PCR products and observing the genotype and size of the alleles; step 4, recording the allele number A, the allele duplication number G, the observed heterozygosity Ho, the desired heterozygosity He, and the accuracy deviation value P based on the Hardy-Weinberg equilibrium law of each prawn, and analyzing the difference of the microsatellite sites; calculating the gene flow Nm according to the frequency parameters of the population allele; gene spacing is determined by using the gene Nei coefficient method. According to the invention, based on the microsatellite analysis method, the method provides a genetic diversity analysis method for a inbreeding male giant freshwater prawn, which is very suitable for researching the genetic structure of different near-parent male prawn groups, and provides a high-quality male parent for the giant freshwater prawns.

The invention provides a method for rotation culture of crawfish and giant fresh water prawn. The method comprises the steps of 1, pond construction and disinfection treatment, 2, aquatic plant cultivating and water body treatment, 3, crawfish stocking and fishing, 4, giant fresh water prawn stocking and fishing, and 5, feeding management; in the crawfish feeding stage, the pH value of the pond is kept between 7.8 and 8.5, the dissolved amount of oxygen in the pond is kept at 5.5 mg / L; in the rearing stage of the giant fresh water prawn: the pH value in the pond is kept between 7.8 and 8.5, and the dissolved amount of oxygen in the pond is kept at 4.5 mg / L. The method for rotation culture of crawfish and giant fresh water prawn raises crawfish and giant fresh water prawn in a reasonable season by rotations, the water body system is reutilized, the utilization rate of the pond is increased, the risk of the farming process is subdued, and the method has a significant economic effect.

The invention discloses a high-density layered intensive cultivation method for giant freshwater prawns. The method comprises the following steps: 1) preparing an aquaculture pond; 2) stocking shrimp larvae; 3) culturing fishes in a mixed form; 4) feeding baits and applying a fertilizer; and 5) fishing. According to the invention, a giant freshwater prawn inhabiting device is arranged in the aquaculture pond. The device is composed of a PVC pipe 1, a wire rope 2, a bamboo chip 3, a buckle 4, meshes 5, a floating ball 6, a weight 7, a water hyacinth 8 and a catkin strip 9. Three layers of meshes are arranged and served as inhabiting beds of the giant freshwater prawns; under the condition that the culture area is not increased, the inhabiting space of the giant freshwater prawns is increased and the mutual interference of the giant freshwater prawns is reduced, so that the culturing density is indirectly increased, the yield is increased and the yield per mu can reach up to above 1100kg; the yield is two times of the yield of a common culturing method; the disease probability of the giant freshwater prawns is greatly reduced; and the survival rate can reach up to above 85%.

The invention provides a compound feed for giant freshwater prawns. The compound feed is prepared from the following components in parts by weight: 27-33 parts of fish meal, 12-18 parts of bean pulp, 10-18 parts of soybean protein powder and 20-25 parts of flour; the compound feed is characterized by further comprising 0.5-3 parts of probiotic promoting substance. After the prawns eat the compound feed, the probiotic promoting substance is capable of promoting the growth of the effective microbial community in the bodies of the prawns so that the prawns can be healthier, grow vigorously, and be stronger. Excess feed thrown in the vigorous growth period of the prawns is dissolved into water and then the probiotic promoting substance in the feed is released into the water to promote the growth of the effective microbial community in the water body, and therefore, the water quality can be improved, the problem of eutrophication caused by the excess feed is solved and a good beneficial cycle is formed.

The invention provides phagostimulant for improving the palatability of fish-meal-free giant freshwater prawn feed. The phagostimulant is prepared from 2,6-dibromophenol premix, arginine, glycine, alanine and silicon dioxide. The phagostimulant is added to the fish-meal-free feed, so the breeding effect of fish-meal-free giant freshwater prawn feed and the breeding effect of commercially-available giant freshwater prawn feed with normal fish meal content have no obvious differences, the cost of fish-meal-free giant freshwater prawn feed is remarkably lower than that of commercially-available feed, and therefore the breeding benefits and incomes can be effectively improved.

The invention provides a mandarin finish breeding method with a puffed compound feed. The mandarin fish breeding method with puffed compound feed comprises the following steps: selecting a pond with an area of 5-15 mu, a water depth of above 2.5m, convenient water intake and water drainage, and good water quality; erecting net cages in the center of the pond, and the net cages are connected with the pond banks through floating bridges; two nano -microporous oxygenation aerators and one feeding area are arranged in each net cage, and 2-4 sets of waterwheel type oxygenation machines are arrangedat the outside of the net cages; adding mandarin fish fry in domestication net cages for domestication until the mandarin fish fry is domesticated to eat the puffed compound feed; 40-60 g of the mandarin fish fry which is adapted to eat feed is sorted into breeding net cages, and are fed with the mandarin fish puffed compound feed; and the outsides of the net cages are matched with aristichthys nobilis fry, silver carp fry, fingerling fry and giant freshwater prawns. The mandarin finish breeding method with the puffed compound feed through erecting net cages in the center of the pond has theadvantages of fast growth rate, neat specification of finished product breeding, low fixed input, easy conditioning of water quality, conveniently catching, and conveniently feeding and the like, cansignificantly improve ecological, economic, and social benefits for the mandarin finish farming industry.

The invention relates to a method for increasing the egg mass rate of giant freshwater prawns. The method comprises the following steps: by taking a columnar PVC tube as an artificial fish nest, transversely placing the PVC tube at the bottom of a breeding container, wherein the PVC tube is 15-30 cm long and has an inner diameter of 4-15 cm; putting prawns which are 10 cm or longer, have completelimbs and swim actively as parents into the breeding container; when the water temperature is 20 DEG C, feeding the prawns, and slowly heating to 28-34 DEG C, wherein the feeding amount accounts for 3-8% of a prawn weight, and the feed comprises 2-8% of an immunopotentiator and 92-98% of a basic feed; feeding twice per day in the morning and at night, and feeding for 40-60 days. By adopting the method, the problems that female parents are slow in growth speed, high in death rate, slow in ovary development and low in egg mass rate in the conventional giant freshwater prawn breeding process canbe effectively solved. The method provided by the invention is simple and low in cost, the survival rate of parent prawns can be greatly increased, and the egg mass rate of the giant freshwater prawnscan be increased to 80% or greater.

The invention provides application of the specificity Kazal type protease inhibitor MRPINK of the male genital system of the giant freshwater shrimp in inhibiting the germ cell activity of the giant freshwater shrimp. By research and adding MRPINK in the germ cell of the giant freshwater shrimp, the germ cell activity can be effectively inhibited, and the egg can not be fertilized; and the similar protease inhibitor is added in the frozen germ cell, the endoenzyme activity of the germ cell is inhibited, and the germ cell is inactivated, so that the invention has important referential meaning for the low-temperature storage of the germ cell.

In the invention described here, the approach is to formulate an edible vaccine based on N-terminal yeast surface display expression systems including S. cerevisiae EBY100 / pYD5-VP28, S. cerevisiae EBY100 / pYD5-VP28-VP24 and S. cerevisiae EBY100 / pYD5-VP24 for preventing shrimps such as L. vannamei, P. monodon and M. rosenbergii species from white spot syndrome virus (WSSV) infection, suggesting that yeast surface display expression system expressing WSSV antigen has potential as a prophylactic treatment for WSSV in shrimps via oral vaccination. The technology developed in this patent application can also be used to produce edible (oral) vaccines for preventing and treating other infectious diseases in animals and human.

The invention discloses a primer and probe mixed liquid for detecting macrobrachium rosenbergii ronivirus-1. The mixed liquid is composed of a primer group A and a Taqman fluorescent probe B. The end5' of the Taqman fluorescent probe B is marked with a fluorescent reporter group, and the end 3' of the Taqman fluorescent probe B is marked with a fluorescent quenching group. The primer group A comprises a primer MrRoV-q119F and a primer MrRoV-q119R, the nucleotide sequence of the primer MrRoV-q119F is shown in the formula of SEQ ID NO: 1, the nucleotide sequence of the primer MrRoV-q119R is shown in the formula of SEQ ID NO: 2, and the nucleotide sequence of the Taqman fluorescent probe B is shown in the formula of SEQ ID NO: 3. The invention discloses a fluorescent quantitative detection kit for macrobrachium rosenbergii ronivirus-1. The kit comprises the mixed liquid, an RT-qPCR reaction solution, a positive quality control material and a negative quality control material, the RT-qPCRreaction solution is a probe-method fluorescent quantitative one-step RT-qPCR reaction solution, the negative quality control material is sterilized normal saline, and the positive quality control material is a carrier containing macrobrachium rosenbergii ronivirus-1 replicase genes. The kit is used for detecting the macrobrachium rosenbergii ronivirus-1 and is high in speed and high in specificity.

The invention relates to a method for establishing a giant freshwater prawn ideal protein mode. The suitable needed amount of each kind of EAA in the fodder in the method is calculated according the following formula: the required amount of each kind of EAA (g16g - 1N) = EAA contrast * (2 - DEL - (NR test / NR contrast)), wherein according to the EAA contrast, the content corresponding to the EAA in the fodder is contrasted; according to the DEL, the deduction rate corresponding to the EAA in group fodder is tested; according to the NR test, the nitrogen deposition (mg gMBW - 1day - 1) of group prawns is contrasted. The method for establishing the giant freshwater prawn ideal protein mode overcomes the defect that in the past, a feed formula can not guarantee the balance of all amino acids, particularly the ten kinds of necessary amino acids, so that the nutritive value of fodder protein is influenced and the cost is high. The method fills the blank related to the ideal protein mode in the nutrient requirement parameters of the giant freshwater prawns, the proportional relation of the ten kinds of necessary amino acids is determined, and low protein high-efficiency environment-friendly giant freshwater prawn compound fodder which is balance in amino acid, high in bioavailability and obvious in price advantage is prepared.

The invention discloses a feed for cultivating a giant freshwater prawn aquatic product, which comprises the following components in parts by weight: 10-20 parts of cat's ear lentil powder, 37-43 parts of corn flour, 15-25 parts of white kidney bean powder, 5-10 parts of torreya nucifera powder, 3-6 parts of Chinese velvet bean extract, 2-4 parts of schisandra extract, 1-3 parts of gallnut extract, 4-7 parts of acacia catechu, 3-6 parts of orobanche coerulescens and 5-8 parts of schizonepeta. By adopting the feed, the giant freshwater prawns are rapid in growth, high in dressing percentage and quick to breed, and output is improved.

The invention discloses a method for alternately breeding South America white shrimps and giant freshwater prawns for three batches in a pond greenhouse. The method comprises the steps of building the pond greenhouse, cleaning and sterilizing the pond greenhouse, breeding the South America white shrimps and the giant freshwater prawns in a small shed, arranging offspring throwing time of the three batches, throwing fodder, managing water quality, supplementing oxygen in a combination mode, preventing and controlling diseases, and fishing the South America white shrimps and giant freshwater prawns of the three batches. The method is characterized in that the small shed is built in the greenhouse, and the offsprings are thrown to a pond after being bred in the small shed in a storing mode; the offsprings of the South America white shrimps of the first batch are thrown from March 18th to March 22nd, the offsprings of the South America white shrimps of the second batch are thrown from July 1st to July 6th, and the offsprings of the giant freshwater prawns of the third batch are thrown from October 20th to October 22nd; the first fishing time of the South America white shrimps of the first batch starts from May 20th to May 25th, and the last fishing time of the South America white shrimps of the first batch and pond clearing start from June 10th to June 20th; the first fishing time of the South America white shrimps of the second batch starts from October 5th to October 10th, and the last fishing time of the South America white shrimps of the second batch and pond clearing start from October 18th to October 20th; the first fishing time of the giant freshwater prawns of the third batch starts from November 18th to November 20th, and the last fishing time of the giant freshwater prawns of the third batch and pond clearing start from December 18th to December 25th.

In the invention described here, the approach is to formulate an edible vaccine based on N-terminal yeast surface display expression systems including S. cerevisiae EBY100 / pYD5-VP28, S. cerevisiae EBY100 / pYD5-VP28-VP24 and S. cerevisiae EBY100 / pYD5-VP24 for preventing shrimps such as L. vannamei, P. monodon and M. rosenbergii species from white spot syndrome virus (WSSV) infection, suggesting that yeast surface display expression system expressing WSSV antigen has potential as a prophylactic treatment for WSSV in shrimps via oral vaccination. The technology developed in this patent application can also be used to produce edible (oral) vaccines for preventing and treating other infectious diseases in animals and human.

The invention discloses an efficient material checking technology for giant freshwater prawn pond culture. The technology comprises the steps of manufacturing a material checker and using the materialcheck for checking materials. The material checker is easy to manufacture, low in cost and capable of being manufactured by a farmer; the using method of the material checker is simple and convenientfor a pond opening to use; the material checking technology is simple and convenient to implement and high in practicability and universality; the material checking technology accurately judges the ingestion condition of giant freshwater prawn, the phenomena of insufficient feed and remaining feed are avoided, the feed utilization efficiency is improved, and therefore healthy and rapid growth ofgiant freshwater prawn is promoted, culture benefits of the farmer are increased, and ecological benefits of the culture environment are increased.

The invention relates to a novel larva-bait separator for Macrobrachium rosenbergii. The separator includes a hopper body, a support, a discharge opening, a plurality of observation windows, and a plurality of sieve plates; the hopper body is cylindrical; the plurality of sieve plates include a 35-mesh sieve plate, a 55-mesh sieve plate, and a 70-mesh sieve plate; the plurality of sieve plates are arranged in the hopper body from top to bottom, and each of the sieve plates is horizontally arranged on a holder arranged in the hopper body; every sieve plate can be drawn out from the hopper body; and positions of an outer wall above the sieve plates, of the hopper body, are provided with one observation window each. The novel larva-bait separator is simple in structure, is clean in bait, is high in separation efficiency, can avoid pollution due to foreign matters, and can achieve up and down separation at the same time.

The invention discloses a method for thickening offspring seeds of giant freshwater prawns by using a double-layer greenhouse. According to the method for thickening the offspring seeds of the giant freshwater prawns by using the double-layer greenhouse, by means that a double-layer greenhouse culture environment is constructed, a stocking intensity is reasonably set up, a seed water allocation pond is constructed, a bottom blow-down device is set up, water is exchanged appropriately every day, early seed dwelling sites are increased, and the like, the thickening rate of early seeds can be greatly increased; and meanwhile, through the heat-preservation effect of the double-layer greenhouse, heat energy consumption can be reduced, and the thickening cost can be reduced. Testing shows that by adopting the thickening method disclosed by the invention, the survival rate of the offspring seeds of the giant freshwater prawns can be increased by 50-80%, and the culture energy consumption costcan be reduced by 35-45%.

The invention discloses a culture cage for giant freshwater prawns. The culture cage comprises a plurality of metal frames, layering nets, separation meshes and peripheral nets. The metal frames are parallel to each other. The layering nets are connected in parallel between every two adjacent metal frames, and the peripheral nets are connected to side faces between the metal frames. The separationmeshes are connected to the metal frames. The metal frames, the layering nets, the separation meshes and the peripheral nets form a culture space with upper and lower layers. Feed platforms are arranged on the layering nets in the culture space, and the feed platforms of the adjacent layering nets are mutually staggered. The layering nets, the separation meshes and the peripheral nets are netting. The culture cage provides a good place for the giant freshwater prawns, production, transport and storage are facilitated, and the survival rate and yield per unit area of the giant freshwater prawns are increased.

The invention discloses amethod of giant freshwater prawn in a pond, and relates to the technical field of giant freshwater prawn. The method comprises the following steps of S1, constructing a juvenile prawn culture pond; S2, stocking preparation; S3, stocking juvenile prawns; S4, comprehensive prevention and treatment of diseases. According to the method, the problems of unscientific management,slow juvenile prawn growth and low juvenile prawn survival rate in the macrobrachium nipponense culture process are solved.

The invention relates to protease-(M.rosenbergii sperm gelatinase-like protease, SGP) with the hydrolyzed gelatin function, which is purified from the sperm crude extract of giant freshwater prawn (M.rosenbergii), and a coded gene and an application thereof. The studies of enzyme related with fertilization on sperms of different species are helpful to understanding the mechanism of crustacean fertilization and the mechanism of the production of reproductive isolation between different species. Based on the theories and the techniques, the relevant researchers can establish a good hybrid system, thus obtaining high yield and fast growth hybrid species.