41 results about "Macrobrachium rosenbergii" patented technology

The invention relates to method of crossbreeding the exotic fresh water crayfish, especially to method of crossbreeding the Luo shi swamp shrimp. The invention introduces the distant Luo shi swamp shrimp sprout A, mating the female parent shrimp and male parent shrimp of the distant Luo shi swamp shrimp sprout A with that of native breed and getting the cross cut B1+ and negative cross B1-; mating the cross cut B1+ and negative cross B1- with the native breed and getting the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-; breeding the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-. The survival rate and seed-accumulating rate of the four kinks of sprout is all good, the composition of water, coarse ash content, coarse protein, coarse fat content, amino acid and aliphatic acid is higher than that of native obviously by applying the technique in this invention, especially the breed-accumulating rate of the cross B2-+ of B1- is increased to 67.25%, the rate of emergence to 94.56%.

The invention relates to macyobrachiun rosebergii larvae basal feed which comprises the components of imported fish meal, domestic fish meal, crude soybean meal, peanut meal, puffing soybean, macyobrachiun rosebergii shell meal, silkworm chrysalis, phospholipid oil, flour, squid paste, calcium dihydrogen, betaine, choline chloride, calcium powder, vitamin c lipid, taurine, inositol, deshell elements, allicin, multiple vitamins and composite mineral elements. The macyobrachiun rosebergii larvae basal feed has the advantages that the material cost is low, breeding cost is reduced effectively, the feed utilization amount is reduced by about 15%, beneficial elements of vitamins, mineral substances and the like are collocated scientifically, the nutrition is balanced and completely meets the nutrition and growth needs of macyobrachiun rosebergii larvae, the growth speed and the immunity of the macyobrachiun rosebergii larvae are increased, and the feed has no hormone or additives, and belongs to a natural green pollution-free product.

The invention discloses a giant freshwater prawn shrimp seed period compound feed and a preparation method thereof. The giant freshwater prawn shrimp seed period compound feed consists of the following raw material ingredients in percentage by weight: 15-20% of Manhattan fish meal, 18-25% of bean pulp, 10-14% of peanut cake, 4-7% of corn protein powder, 20-25% of high gluten flour, 1-3% of molasses yeast, 1-3% of squid paste, 0-1% of salt, 0-0.5% of monocalcium phosphate, 1-2% of fish oil, 1-2% of soyabean lecithin, 1% of vitamin premix and 1% of mineral substance premix. The compound feed disclosed by the invention has the advantages of simple proportion and balanced nutrition, the survival rate of the giant freshwater prawn shrimp seed period can be obviously improved, and the growth performance of the giant freshwater prawn can be improved.

Provided is a shrimp-vegetable-fish three-dimensional ecological culture method. The method comprises: firstly, using quick lime to clean a pond and disinfect; exposing a culture pond in the sun for one week, after water injection, putting shrimp fries in the pond, 20-30 days after putting the shrimp fries, putting grass carp, silver carp, spotted silver carp, carp, and giant freshwater prawns inthe pond; feeding South America white shrimp fodder, through observation, determining feeding amount of the fodder, not feeding fodder of other culture varieties; through a floating bed, planting water spinach on a water surface, since prawns are cultured in the pond, pond water is eutrophic, using root systems of the water spinach to absorb eutrophic substances in the pond water, applying fertilizer for the pond being not needed. Three-dimensional ecological efficiency among planted and cultured creatures is used, ecological three-dimensional effect among animals, plants, and microorganisms is used, and heterosis (antagonism) effect is used. A water surface space is fully used, nutrient substances in the water are reasonably used, incomplete decomposition of organic materials in the pondis reduced, occurrence of diseases is reduced, and comprehensive benefits of pond culture is improved. The method has little investment, and is simple in maintenance, and low in manufacturing and operation cost.

The invention discloses a genetic diversity analysis method for a inbreeding male giant freshwater prawn, which comprises the following steps of: step 1, acquiring inbreeding giant freshwater prawn samples with different traits, and extracting the DNA; step 2, selecting a microsatellite marker combination and designing a specific PCR primer set for amplification; step 3, evaluating the number of PCR products and observing the genotype and size of the alleles; step 4, recording the allele number A, the allele duplication number G, the observed heterozygosity Ho, the desired heterozygosity He, and the accuracy deviation value P based on the Hardy-Weinberg equilibrium law of each prawn, and analyzing the difference of the microsatellite sites; calculating the gene flow Nm according to the frequency parameters of the population allele; gene spacing is determined by using the gene Nei coefficient method. According to the invention, based on the microsatellite analysis method, the method provides a genetic diversity analysis method for a inbreeding male giant freshwater prawn, which is very suitable for researching the genetic structure of different near-parent male prawn groups, and provides a high-quality male parent for the giant freshwater prawns.

The invention provides a method for rotation culture of crawfish and giant fresh water prawn. The method comprises the steps of 1, pond construction and disinfection treatment, 2, aquatic plant cultivating and water body treatment, 3, crawfish stocking and fishing, 4, giant fresh water prawn stocking and fishing, and 5, feeding management; in the crawfish feeding stage, the pH value of the pond is kept between 7.8 and 8.5, the dissolved amount of oxygen in the pond is kept at 5.5 mg/L; in the rearing stage of the giant fresh water prawn: the pH value in the pond is kept between 7.8 and 8.5, and the dissolved amount of oxygen in the pond is kept at 4.5 mg/L. The method for rotation culture of crawfish and giant fresh water prawn raises crawfish and giant fresh water prawn in a reasonable season by rotations, the water body system is reutilized, the utilization rate of the pond is increased, the risk of the farming process is subdued, and the method has a significant economic effect.

The invention discloses a high-density layered intensive cultivation method for giant freshwater prawns. The method comprises the following steps: 1) preparing an aquaculture pond; 2) stocking shrimp larvae; 3) culturing fishes in a mixed form; 4) feeding baits and applying a fertilizer; and 5) fishing. According to the invention, a giant freshwater prawn inhabiting device is arranged in the aquaculture pond. The device is composed of a PVC pipe 1, a wire rope 2, a bamboo chip 3, a buckle 4, meshes 5, a floating ball 6, a weight 7, a water hyacinth 8 and a catkin strip 9. Three layers of meshes are arranged and served as inhabiting beds of the giant freshwater prawns; under the condition that the culture area is not increased, the inhabiting space of the giant freshwater prawns is increased and the mutual interference of the giant freshwater prawns is reduced, so that the culturing density is indirectly increased, the yield is increased and the yield per mu can reach up to above 1100kg; the yield is two times of the yield of a common culturing method; the disease probability of the giant freshwater prawns is greatly reduced; and the survival rate can reach up to above 85%.

The invention provides a compound feed for giant freshwater prawns. The compound feed is prepared from the following components in parts by weight: 27-33 parts of fish meal, 12-18 parts of bean pulp, 10-18 parts of soybean protein powder and 20-25 parts of flour; the compound feed is characterized by further comprising 0.5-3 parts of probiotic promoting substance. After the prawns eat the compound feed, the probiotic promoting substance is capable of promoting the growth of the effective microbial community in the bodies of the prawns so that the prawns can be healthier, grow vigorously, and be stronger. Excess feed thrown in the vigorous growth period of the prawns is dissolved into water and then the probiotic promoting substance in the feed is released into the water to promote the growth of the effective microbial community in the water body, and therefore, the water quality can be improved, the problem of eutrophication caused by the excess feed is solved and a good beneficial cycle is formed.

The invention provides phagostimulant for improving the palatability of fish-meal-free giant freshwater prawn feed. The phagostimulant is prepared from 2,6-dibromophenol premix, arginine, glycine, alanine and silicon dioxide. The phagostimulant is added to the fish-meal-free feed, so the breeding effect of fish-meal-free giant freshwater prawn feed and the breeding effect of commercially-available giant freshwater prawn feed with normal fish meal content have no obvious differences, the cost of fish-meal-free giant freshwater prawn feed is remarkably lower than that of commercially-available feed, and therefore the breeding benefits and incomes can be effectively improved.

The invention provides a mandarin finish breeding method with a puffed compound feed. The mandarin fish breeding method with puffed compound feed comprises the following steps: selecting a pond with an area of 5-15 mu, a water depth of above 2.5m, convenient water intake and water drainage, and good water quality; erecting net cages in the center of the pond, and the net cages are connected with the pond banks through floating bridges; two nano -microporous oxygenation aerators and one feeding area are arranged in each net cage, and 2-4 sets of waterwheel type oxygenation machines are arrangedat the outside of the net cages; adding mandarin fish fry in domestication net cages for domestication until the mandarin fish fry is domesticated to eat the puffed compound feed; 40-60 g of the mandarin fish fry which is adapted to eat feed is sorted into breeding net cages, and are fed with the mandarin fish puffed compound feed; and the outsides of the net cages are matched with aristichthys nobilis fry, silver carp fry, fingerling fry and giant freshwater prawns. The mandarin finish breeding method with the puffed compound feed through erecting net cages in the center of the pond has theadvantages of fast growth rate, neat specification of finished product breeding, low fixed input, easy conditioning of water quality, conveniently catching, and conveniently feeding and the like, cansignificantly improve ecological, economic, and social benefits for the mandarin finish farming industry.

The invention relates to a method for increasing the egg mass rate of giant freshwater prawns. The method comprises the following steps: by taking a columnar PVC tube as an artificial fish nest, transversely placing the PVC tube at the bottom of a breeding container, wherein the PVC tube is 15-30 cm long and has an inner diameter of 4-15 cm; putting prawns which are 10 cm or longer, have completelimbs and swim actively as parents into the breeding container; when the water temperature is 20 DEG C, feeding the prawns, and slowly heating to 28-34 DEG C, wherein the feeding amount accounts for 3-8% of a prawn weight, and the feed comprises 2-8% of an immunopotentiator and 92-98% of a basic feed; feeding twice per day in the morning and at night, and feeding for 40-60 days. By adopting the method, the problems that female parents are slow in growth speed, high in death rate, slow in ovary development and low in egg mass rate in the conventional giant freshwater prawn breeding process canbe effectively solved. The method provided by the invention is simple and low in cost, the survival rate of parent prawns can be greatly increased, and the egg mass rate of the giant freshwater prawnscan be increased to 80% or greater.

The invention relates to a method for establishing a giant freshwater prawn ideal protein mode. The suitable needed amount of each kind of EAA in the fodder in the method is calculated according the following formula: the required amount of each kind of EAA (g16g - 1N) = EAA contrast * (2 - DEL - (NR test/NR contrast)), wherein according to the EAA contrast, the content corresponding to the EAA in the fodder is contrasted; according to the DEL, the deduction rate corresponding to the EAA in group fodder is tested; according to the NR test, the nitrogen deposition (mg gMBW - 1day - 1) of group prawns is contrasted. The method for establishing the giant freshwater prawn ideal protein mode overcomes the defect that in the past, a feed formula can not guarantee the balance of all amino acids, particularly the ten kinds of necessary amino acids, so that the nutritive value of fodder protein is influenced and the cost is high. The method fills the blank related to the ideal protein mode in the nutrient requirement parameters of the giant freshwater prawns, the proportional relation of the ten kinds of necessary amino acids is determined, and low protein high-efficiency environment-friendly giant freshwater prawn compound fodder which is balance in amino acid, high in bioavailability and obvious in price advantage is prepared.

The invention relates to a giant freshwater prawn feed test method. The method includes the following steps: 1) selecting giant freshwater prawns with the initial body mass of 1.36g +- 0.06g, and theinitial body length of 3.95cm +- 0.06cm; 2) feed test: adopting soybean meal, fishmeal, peanut meal and meat powder as a protein source to prepare a base feed according to the nutritional requirementof the giant freshwater prawns, adopting the base feed as a control group; replacing 0%, 9.15%, 18.25% and 27.35% of fishmeal in the base feed with 0%, 5%, 10% and 15% of corn gluten meal in a test feed; 3) feeding management; 4) determination of 4.1) growth performance indicators; 4.2) nutrient indicators; 5) statistical analysis. According to the method, the feasibility of application of the corn gluten meal in the feed of the giant freshwater prawns is achieved, and a basis for the application of the corn gluten meal in the feed of the giant freshwater prawns is provided.

The invention discloses a feed for cultivating a giant freshwater prawn aquatic product, which comprises the following components in parts by weight: 10-20 parts of cat's ear lentil powder, 37-43 parts of corn flour, 15-25 parts of white kidney bean powder, 5-10 parts of torreya nucifera powder, 3-6 parts of Chinese velvet bean extract, 2-4 parts of schisandra extract, 1-3 parts of gallnut extract, 4-7 parts of acacia catechu, 3-6 parts of orobanche coerulescens and 5-8 parts of schizonepeta. By adopting the feed, the giant freshwater prawns are rapid in growth, high in dressing percentage and quick to breed, and output is improved.

The invention discloses an efficient material checking technology for giant freshwater prawn pond culture. The technology comprises the steps of manufacturing a material checker and using the materialcheck for checking materials. The material checker is easy to manufacture, low in cost and capable of being manufactured by a farmer; the using method of the material checker is simple and convenientfor a pond opening to use; the material checking technology is simple and convenient to implement and high in practicability and universality; the material checking technology accurately judges the ingestion condition of giant freshwater prawn, the phenomena of insufficient feed and remaining feed are avoided, the feed utilization efficiency is improved, and therefore healthy and rapid growth ofgiant freshwater prawn is promoted, culture benefits of the farmer are increased, and ecological benefits of the culture environment are increased.

The invention relates to a novel larva-bait separator for Macrobrachium rosenbergii. The separator includes a hopper body, a support, a discharge opening, a plurality of observation windows, and a plurality of sieve plates; the hopper body is cylindrical; the plurality of sieve plates include a 35-mesh sieve plate, a 55-mesh sieve plate, and a 70-mesh sieve plate; the plurality of sieve plates are arranged in the hopper body from top to bottom, and each of the sieve plates is horizontally arranged on a holder arranged in the hopper body; every sieve plate can be drawn out from the hopper body; and positions of an outer wall above the sieve plates, of the hopper body, are provided with one observation window each. The novel larva-bait separator is simple in structure, is clean in bait, is high in separation efficiency, can avoid pollution due to foreign matters, and can achieve up and down separation at the same time.

The invention discloses a method for thickening offspring seeds of giant freshwater prawns by using a double-layer greenhouse. According to the method for thickening the offspring seeds of the giant freshwater prawns by using the double-layer greenhouse, by means that a double-layer greenhouse culture environment is constructed, a stocking intensity is reasonably set up, a seed water allocation pond is constructed, a bottom blow-down device is set up, water is exchanged appropriately every day, early seed dwelling sites are increased, and the like, the thickening rate of early seeds can be greatly increased; and meanwhile, through the heat-preservation effect of the double-layer greenhouse, heat energy consumption can be reduced, and the thickening cost can be reduced. Testing shows that by adopting the thickening method disclosed by the invention, the survival rate of the offspring seeds of the giant freshwater prawns can be increased by 50-80%, and the culture energy consumption costcan be reduced by 35-45%.

The invention relates to protease-(M.rosenbergii sperm gelatinase-like protease, SGP) with the hydrolyzed gelatin function, which is purified from the sperm crude extract of giant freshwater prawn (M.rosenbergii), and a coded gene and an application thereof. The studies of enzyme related with fertilization on sperms of different species are helpful to understanding the mechanism of crustacean fertilization and the mechanism of the production of reproductive isolation between different species. Based on the theories and the techniques, the relevant researchers can establish a good hybrid system, thus obtaining high yield and fast growth hybrid species.