The present invention discloses a Chinese herbal medicinal preparation with stable medicine properties for immunological enhancement and sterilization of penaeus orientalis and a method thereof. The Chinese herbal medicinal preparation comprises the following ingredients in mass percentages: 20%-50% of astragalus polysaccharide, 20%-50% of chlorogenic acid, 20%-50% of allicin, 5%-10% of sodium hydrogen sulfite, and 3%-6% of dimethyl-beta-propionic acid thialdine. The method comprises the following steps: radix astragali is crushed, the crushed radix astragali is screened, the screened radix astragali is mixed evenly with distilled water to be boiled, filtrate is concentrated, ethanol is added until the ethanol content is 80%, and the materials are subjected to suction filtration overnight to obtain the astragalus polysaccharide; cortex eucommiae is crushed, the crushed cortex eucommiae is screened, the screened cortex eucommiae is mixed evenly with ethyl acetate, the mixture is subjected to stirring and reflux extracting at water bath at 65 DEG C, filtrates are blended, ethyl acetate is separated, and the chlorogenic acid is obtained. The Chinese herbal medicinal preparation is simple in preparation processes, relatively low in costs and convenient in applications, can effectively improve the immune defense ability and sterilization function of the penaeus orientalis after the production application, effectively prevents and cures diseases of the penaeus orientalis, reduces the uses of antibiotics and chemical drugs, provides technical supports for sustainable development of breeding health of the penaeus orientalis, and provides technical supports for ecological environment protection of water areas.