248 results about "Pine wood" patented technology

The invention discloses a pine wood nematode disease intelligent identification management and control method and system based on artificial intelligence, and relates to the technical field of forest management and control, and the system discloses a pine forest region division management and control module, a pine wood nematode disease identification module, a pine wood nematode disease treatment analysis module, and a region management and control sorting module. A pine forest region division control module and a pine wood nematode disease identification module are arranged, reasonable region control is performed on a pine forest region, pine wood nematode diseases in the pine forest region are accurately identified through deep analysis of multi-dimensional disease features, and a pine wood nematode disease treatment analysis module and a region control sorting module are arranged. The pine forest simulation management and control model of the pine wood nematode disease recognition area is constructed, the actual situation of the pine wood nematode disease recognition area in the subsequent management and control process is accurately analyzed, and the current disease situation, the subsequent disease control situation and the disease influence situation of the pine wood nematode disease recognition area are quantitatively analyzed.

The invention discloses a pest control management system and method for forest tree breeding, and relates to the technical field of forest tree breeding and seedling raising, and the method comprises the following steps: collecting environment data and soil rhizosphere data by collecting multi-mode images of leaves and trunks of a pinus massoniana young forest and videos that pinus massoniana caterpillars gnaw leaves and cluster diffusion; constructing a disease and insect pest recognition model by using a convolutional neural network, performing definite diagnosis on pine wood nematode disease, pine caterpillars and monochamus alternatus hope, and quantifying the disease and insect pest occurrence area and population density of each sub-region; calculating a pine wood nematode disease control demand index, comparing the pine wood nematode disease control demand index with a threshold value, and triggering a corresponding control strategy; the diffusion coefficient of the pine caterpillars is calculated and compared with a risk threshold value, and a prevention and control scheme is formulated; calculating the propagation coefficient of the monochamus alternatus hope, judging the propagation risk and giving a prevention and control strategy; according to the invention, intelligent pest identification, risk quantification and control strategy closed-loop control are realized, treatment and scientific prevention and control are facilitated, and the pest control efficiency of the pinus massoniana young forest and the forest stand health level are improved.

The invention relates to the field of agricultural protection preparations, in particular to preparation of gold nanoclusters and application of the gold nanoclusters to prevention and treatment of plant parasitic nematodes. The invention provides a preparation method of a gold nanocluster, which comprises the following steps: preparing by using HAuCl4 and L-His to obtain an L-type histidine modified gold nanocluster; the preparation method comprises the steps that a HAuCl4 aqueous solution and an L-His aqueous solution are evenly mixed and then incubated, an obtained reaction solution containing L-His-AuNCs is centrifuged and then subjected to aftertreatment such as ultrafiltration, and the gold nanocluster is obtained. The gold nanocluster can be used for preventing and treating pine wood nematodes.

The present disclosure provides a method for identifying a pine wood nematode-infected discolored wood in a mixed coniferous and broadleaf forest, including inputting a pine forest image of the mixed coniferous and broadleaf forest to be identified into a trained identification model for the pine wood nematode-infected discolored wood to identify the pine wood nematode-infected discolored wood in the mixed coniferous and broadleaf forest, wherein the trained identification model is improved based on a you Only Look Once version 5 small (YOLOv5s) model by connecting a feature-filtering module after a Neck; constructing a feature-enhancing module to replace a C3 module in a Backbone; constructing a convolution-transformer module based on multi-head self-attention (MSHA) to connect after a last layer of residual units in the Backbone; constructing, by Group Shuffle Convolution (GSConv), a multi-scale feature fusion layer to replace an ordinary convolution in the Neck.

The invention discloses a pesticide compound composition of abamectin and dsRNA (double-stranded ribonucleic acid) and application of the pesticide compound composition in prevention and control of nematodes. According to the invention, dsRNA is respectively designed and synthesized for five Mcm genes of bursaphelenchus xylophilus, and nucleotide sequences of positive-sense strands of the dsRNA are respectively shown as SEQ ID No.1-SEQ ID No.5; the synthesized dsRNA and abamectin of different components are compounded to obtain the pesticide compound composition, the pesticide compound composition shows a remarkable synergistic effect in the aspect of preventing and treating pine wood nematode diseases, and the nematode killing effect of combined use of the dsRNA and the abamectin is superior to that of single use of any agent; the pesticide compound composition has the potential to become a novel trunk injection for preventing the pine wood nematode disease.

The invention provides application of a pseudomonas chlororaphis strain Rhs-P27 in prevention and treatment of pine wood nematode diseases, and relates to the technical field of agricultural environmental microorganisms. In particular to a Pseudomonas chlororaphis strain Rhs-P27 separated from rhizosphere soil of Chinese pines and application of the Pseudomonas chlororaphis strain Rhs-P27 in prevention and treatment of pine wood nematode diseases. The strain is preserved in the China Center for Type Culture Collection in Wuhan University on December 18, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) 20242846. The in-vitro poisoning activity of the strain Rhs-P27 on pine wood nematodes can reach 100% within 24 hours; a fermentation filtrate and a zymophyte suspension of the strain Rhs-P27 have a remarkable inhibition effect on the propagation of the pine wood nematodes; the prevention effect of a zymophyte suspension of the strain Rhs-P27 on the pine wood nematode disease of biennial pinus tabulaeformis potted seedlings can reach 45%, and the density of pine wood nematode in pine trees can be remarkably reduced; the pseudomonas chlororaphis strain Rhs-P27 disclosed by the invention has a good application prospect in the field of pollution-free prevention and treatment of pine wood nematode diseases.

The invention relates to a preparation method and application of carbon nanosphere modified biomass monolithic composite porous carbon, and belongs to the field of energy and chemical industry, and the preparation method comprises the following steps: S1, cutting pine into pine chips, drying, and carbonizing at high temperature to obtain a carbonized product; s2, dipping the carbonized product in a hydrochloric acid solution, then washing the carbonized product to be neutral, dehydrating the carbonized product, and polishing the dehydrated carbonized product; s3, putting the composite porous carbon into a glucose solution, carrying out vacuum-assisted impregnation, transferring into a hydrothermal reaction kettle, carrying out hydrothermal carbonization, drying, and carrying out constant-temperature chemical conversion; and S4, placing the composite porous carbon in a potassium bicarbonate solution for vacuum impregnation, drying and dehydrating, placing in a tubular furnace for constant-temperature chemical conversion to obtain a converted and carbonized product, impregnating in hydrochloric acid, washing, and drying. The specific surface area of the integral composite porous carbon is effectively increased, the electrode / electrolyte interface contact characteristic is remarkably optimized, and the pore channel space utilization rate and the energy storage performance are synchronously improved.

The utility model relates to the technical field of laser cutting, in particular to a pine board laser cutting smoke dust adsorption device which comprises a cutting platform, a smoke suction assembly and a smoke guide cover, a moving mechanism is arranged on the cutting platform, and a laser cutting device is arranged on the moving mechanism. The smoke suction assembly is arranged on the moving mechanism, synchronously moves along with the moving mechanism and is used for adsorbing smoke dust generated during laser cutting, the smoke suction assembly comprises an adsorption mechanism and a smoke suction nozzle connected with the output end of the adsorption mechanism, and the smoke guide cover is arranged on the periphery of the laser cutting equipment. Smoke dust generated during laser cutting is guided upwards through the smoke guide cover and then adsorbed upwards, a specific flowing path is provided for the smoke dust, the adsorption effect is optimized, the smoke suction assembly is prevented from getting close to laser cutting equipment, and the smoke suction assembly is prevented from getting close to the laser cutting equipment. The influence of negative pressure generated when the smoke suction assembly sucks smoke on airflow near the cutting head is reduced.

The invention relates to the technical field of organic chemistry, in particular to application of pyrazine compounds in the aspect of killing nematodes. The pyrazine compound has good nematicidal activity on pine wood nematodes, root-knot nematodes and aphelenchoides besseyi, and part of the pyrazine compound also has good activity on root knot nematode diseases caused by the root-knot nematodes, so that the pyrazine compound has good application prospects in the aspect of root knot nematode disease resistance.

The invention relates to the technical field of pest prevention and control, in particular to a method for treating pine wood nematode infected wood through poria cocos. The method comprises the following steps: cutting down current-year epidemic wood, and treating tree heads, tree trunks and boughs to complete peeling and tendon retaining treatment; uniformly cutting the trunk and the boughs into standard cut-logs; the method comprises the following steps: selecting fresh poria cocos sclerotium skin tissues as strains, and cutting according to an area greater than 50% of a cross section; pit pits are dug in the sunny slope; laying small branches on the bottom layer, placing the cut-logs in the pits, fixing the cut-logs with non-woven fabrics after inoculation, covering the upper layer with thin branches and leaves, and covering with soil; the two-stage biological process of mycelium planting, propagation expanding and pine wood nematode and medium insect killing is sequentially completed through a soil heat and moisture preservation environment; the propagation path of pine wood nematodes is blocked through the natural isolation effect of soil, deadwood obtained after poria cocos is subjected to biological verification and then smashed on site to return to forests or be made into fuel particles, and harmless and resource recycling of epidemic wood is achieved. According to the method, the problems of pine wood nematode propagation and epidemic wood treatment are synchronously solved.

The utility model relates to the technical field of forestry pest prevention and control, in particular to a pesticide injection device for preventing and controlling pine wood nematode disease, which comprises a main body, a first rotating groove and a second rotating groove are arranged at the top of the main body, and a first miniature water pump shell is rotatably connected in the second rotating groove. A second fixing block is fixedly connected to the end, away from the first micro water pump shell, of the main body, the first micro water pump shell, the main body and the second fixing block are connected through a second hose, and a second micro water pump shell is rotationally connected into the first rotating groove; a first fixing block is fixedly connected to the end, away from the second micro water pump shell, of the main body, and the second micro water pump shell, the main body and the first fixing block are connected through a first hose. Through the design, the overall practicability of the medicine injection device for preventing and treating the pine wood nematode disease can be improved.

The invention discloses a bacillus tequilensis strain HN-1 and application thereof, and belongs to the technical field of biological control of plant parasitic nematodes. The strain HN-1 is identified as bacillus tequilensis, the preservation date is July 28, 2025, the strain HN-1 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20251718, and the preservation number is CCTCC NO: M 20251718. The invention discovers that the bacillus tequilensis strain HN-1 has good insecticidal activity on meloidogyne incognita and pine wood nematode for the first time; the fermentation liquor and fermentation metabolites of the strain HN-1 can be used as a liquid fungicide, or the fermentation liquor is used for preparing a solid fungicide, and the fungicide can be used for preventing and treating meloidogyne incognita and pine wood nematodes in fields.

Belongs to the technical field of pine wood nematode disease prevention and control, and particularly relates to a preparation method and application of a pyrazole-chitosan oligosaccharide derivative. Chloroacetyl chloride serves as an acylating agent, chitosan oligosaccharide is acetylated, and chloroacetyl chitosan oligosaccharide is generated; then, a pyrazole molecule with nematicidal activity is connected to the Cl site of the chloracetyl chitosan oligosaccharide through ammonolysis reaction, and the novel pyrazole-chitosan oligosaccharide derivative is obtained. The derivative has relatively high contact activity on pine wood nematodes, and LC50 of 24 hours and LC50 of 48 hours are 4.814 mM and 4.061 mM respectively; the chitosan oligosaccharide can remarkably inhibit multiple physiological indexes such as movement, feeding and service life of the pine wood nematodes, and is good in stability, low in molecular weight of the chitosan oligosaccharide, good in water solubility, green and environment-friendly, and the plant toxicity is remarkably reduced. The derivative has a good application prospect in the aspect of controlling the pine wood nematode disease and can be applied to prevention and control of the pine wood nematode disease.

The invention provides an abnormal color-changing pine monitoring method and system, and particularly relates to the technical field of monitoring and evaluation.The method comprises the steps that firstly, an unmanned aerial vehicle remote sensing image of a target forest area is obtained, and a preprocessing data set containing abnormal color-changing pine and withered pine labels is generated through clipping, framing and labeling processing; the data set is input into a target detection model for training, the model comprises a feature extraction layer based on a lightweight convolutional network, an attention mechanism layer integrating a channel and a space attention sub-layer, and a feature fusion layer adopting a bidirectional weighted feature pyramid structure, and finally a pine tree state recognition model is obtained. Based on pine tree coordinate data output by the model, multi-factor weight collaborative analysis is carried out in combination with multi-source environmental factors, a pine wood nematode disease diffusion risk probability graph is generated, and monitoring and early warning information is output according to the pine wood nematode disease diffusion risk probability graph. And efficient and accurate pine tree disease monitoring is realized.

The invention discloses a compound composition of fluopyram and dsRNA (double-stranded ribonucleic acid) and application of the compound composition in prevention and control of nematodes. The method comprises the following steps: soaking pine wood nematodes by adopting a fluopyram medicament with LC20 concentration, collecting samples at different time points respectively, and carrying out transcriptome analysis. The pesticide compound composition for effectively poisoning and killing the pine wood nematodes is obtained by comparing gene expression differences at different time points, screening out remarkably up-regulated detoxification genes BxCYP, BxCES and BxUDP, designing and synthesizing double-stranded RNA (ds BxCYP, ds BxCES and ds BxUDP) according to the detoxification genes, and compounding the double-stranded RNA with fluopyram. The pesticide compound composition has a remarkable synergistic prevention and control effect on pine wood nematodes, is expected to become a novel and efficient pine wood nematode prevention and control agent, and provides a new strategy and theoretical basis for prevention and control of pine wood nematode diseases.

The invention relates to an unmanned aerial vehicle pine wood nematode disease detection method based on multi-modal features. The method comprises the following steps: constructing an image-text multi-modal data set to provide prior semantic knowledge; using vegetation index threshold mask filtering (VTMF) to enhance spectral difference and suppress noise; frequency domain denoising and feature retention are carried out by using a W-KAN module fusing wavelet transform and KAN attention; modeling a long-distance spatial dependency relationship through a four-domain diffusion diagram convolution residual attention module (Q-DGCRAB); an epidemic situation semantic enhancement detection module (ESADM) is introduced to realize text-visual feature fusion; the training process is dynamically optimized by adopting a forest guardian collaborative optimization algorithm (FGA-CE); according to the method, the problem of low pine wood nematode disease detection precision caused by complex background, repeated texture, significant noise and insufficient semantic information in a high-altitude unmanned aerial vehicle image is solved, the pine wood nematode disease detection precision and robustness in a complex forest stand environment are significantly improved, and the pine wood nematode disease can be accurately monitored in early stage in real time.

The invention discloses a durable bio-based asphalt waterproof coiled material and a preparation method thereof, and relates to the field of waterproof coiled materials. The composite material is formed by coating a base cloth with modified bio-based asphalt, the modified bio-based asphalt is lignin-modified bio-based asphalt, the bio-based asphalt is filled with oil-immersed wood fibers, and the oil-immersed wood fibers are formed by impregnating pine fibers and nano naphthenic oil. The nano naphthenic oil is prepared by mixing nano zinc oxide and naphthenic oil; through triple synergy of lignin modification, wood fiber reinforced oil locking and nano zinc oxide slow-release corrosion prevention, the purpose that the environment-friendly property and the high-performance index of the bio-based material are achieved at the same time is achieved.

The attractant is composed of (1R)-2, 2-dimethyl-3-methylene dicycloheptane, pinene, camphene and other components, 95% ethyl alcohol is used as a solvent, porous silica gel microspheres with the average pore size of 2-5 nm are matched to serve as a slow-release carrier, and the pine tree longicorn attractant is prepared. The preparation method comprises the steps of step-by-step mixing, ultrasonic dispersion, standing reaction and slow-release packaging, and process parameters of each step are accurately controlled. Compared with the prior art, the pine tree longhorn beetle attractant has the remarkable advantages that through precise matching of the components and collaborative optimization of technological parameters, triple breakthrough of the pine tree longhorn beetle attractant in targeting, stability and cost control is achieved, and an efficient and reliable technical scheme is provided for green prevention and control of pine wood nematode diseases.

The invention discloses bacillus safensis and application thereof in prevention and control of pine wood nematode diseases, and belongs to the technical field of biocontrol of plant diseases. The invention discloses bacillus safensis GDWS1 which is separated from resistant masson pine tissues and has a relatively good inhibition effect on pine wood nematode diseases, and the preservation number of the bacillus safensis GDWS1 is GDMCC No: 66264. The strain GDWS1 has high lethal activity on pine wood nematodes, the correction death rate of the strain GDWS1 on the pine wood nematodes reaches 90% or above, and active substances capable of poisoning and killing the pine wood nematodes and good in stability can be generated; the requirements on culture conditions are low, and the ecological environment is friendly and pollution-free. A greenhouse control experiment is carried out by using pinus massoniana seedlings, the strain GDWS1 can effectively reduce the disease index of the pinus massoniana seedlings inoculated with pine wood nematodes, the control effect reaches 45.88%, and the strain has a good application prospect in prevention and control of pine wood nematode diseases, provides a new biocontrol bacterium type for biological prevention and control of pine wood nematode diseases, and has the potential of being developed into biopesticides.

The utility model discloses improved pine cultivation equipment which comprises a cultivation frame, a plurality of basin fixing hoops are installed on the cultivation frame, cultivation basins are placed in the basin fixing hoops, a water guide drawer is arranged below the cultivation basins, and erecting columns are arranged on the two sides of the water guide drawer. A pot body is arranged in the culture pot, connecting pipes are installed on the two sides of the pot body, an air conveying disc is arranged between the connecting pipes, air holes are formed in the outer surface of the pot body, and a plurality of drainage holes are formed in the bottom of the pot body. Pine seedlings are placed in a culture pot, in order to improve pine and grow vigorously, an oxygen supply device can be connected to an input cylinder, a hand wheel is controlled by the input cylinder to rotate, oxygen is input into an air conveying disc from a connecting pipe, oxygen is supplied into soil from a guide column, and therefore the roots and stems of the pine seedlings can absorb oxygen, and the roots and stems of the pine seedlings can grow vigorously. And meanwhile, the other connecting pipe can be connected with a medicament, and the medicament is sprayed between the guide columns, so that the medicament can be absorbed by the soil more sufficiently, and the culture effect of the pine wood is further improved.

The invention relates to the technical field of agricultural microorganisms, in particular to bacillus tequilensis R78 for preventing and treating pine wood nematode diseases and application of the bacillus tequilensis R78. The bacillus tequilensis R78 for preventing and treating the pine wood nematode disease is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32106, and the preservation time is September 29, 2024. The bacillus tequilensis R78 can be used for preventing and treating the pine wood nematode disease. The bacillus tequilensis R78 is separated from pine trees, and fermentation liquor of the bacillus tequilensis R78 has stable and efficient pine wood nematode inhibiting and killing capacity. When OD600 is equal to 1.5, the fatality rate of fermentation liquor of the strain R78 on pine wood nematode can reach 95% or above, the disease index of pine tree infected with pine wood nematode disease can be remarkably reduced, and the prevention and treatment effect can reach 68.6%.

The pine wood nematode disease unmanned aerial vehicle monitoring and positioning device comprises an unmanned aerial vehicle body, a mounting block is mounted at the bottom of the unmanned aerial vehicle body, and an auxiliary flight stabilizing structure for improving flight stability by means of airflow is arranged in the mounting block; the bottom of the mounting block is provided with a plugging structure for cooperating with the auxiliary flight stabilization structure, and the bottom of the mounting block is connected with a box body in the middle of the plugging structure. According to the pine wood nematode disease unmanned aerial vehicle monitoring and positioning device and method, cooperative work of the flight stabilizing structure and the blocking and triggering structure is assisted, an airflow channel is optimized, the flight stability of the unmanned aerial vehicle under the conditions of complex terrains and severe weather is enhanced, energy consumption is reduced, and the endurance time is prolonged. In addition, the device is provided with a wireless communication module and a network adapter, and collected and processed data can be transmitted to a ground control station or a cloud server in real time.

The utility model provides a high-temperature killing device for killing termites, and relates to the technical field of termite killing, the high-temperature killing device for killing termites comprises a cylinder, four side surfaces of the cylinder are uniformly provided with a plurality of termite inlets, the bottom of the inner wall of the cylinder is fixedly connected with a support plate, the middle of the top of the support plate is fixedly connected with a fuse, and the top of the support plate is fixedly connected with a water inlet. The top of the cylinder body is provided with a cover body, a fixing device is arranged between the cover body and the cylinder body, the top of the cover body is fixedly connected with a threaded ring, the two auxiliary plates are arranged, under the action of the threaded rod, the threaded rod can drive the auxiliary plates to move, the auxiliary plates can drive pine wood to move towards the fuze in the moving process, and therefore the fuze can be fixed to the pine wood. The auxiliary plate can limit the pine until the pine is in contact with the fuze, so that the pine can be prevented from inclining and being away from the fuze, the pine can be ignited together when the fuze is ignited, and then the termites can be normally killed.

This invention discloses a multi-source data fusion early warning and precision application method and system for pine wilt disease, belonging to the field of smart forestry technology. It includes a multi-source data acquisition module, a spatiotemporal data fusion module, a health index calculation module, an external feature extraction module, a dual-channel coupled diagnosis module, a graded early warning module, an application urgency assessment module, an application strategy planning module, and an adaptive optimization module. This invention collects and fuses multi-source data such as satellite remote sensing data, UAV inspection data, ground-based IoT data, and forest meteorological data. It utilizes a dual-channel coupled deep learning model to achieve probabilistic diagnosis of the multidimensional state of individual trees and to extrapolate the spread of the disease. Based on the diagnostic results, it generates graded differentiated application strategies and finally achieves adaptive closed-loop optimization of the calculation parameters and model through a control effect feedback mechanism.

The invention relates to a multi-channel controllable pine wood nematode collection culture dish and a culture and collection method, and belongs to the technical field of pine wood nematode culture.The multi-channel controllable pine wood nematode collection culture dish comprises a culture dish body, the culture dish body comprises a culture dish cover and a culture dish body, the culture dish cover is provided with three sampling holes, sampling pipes are installed at the sampling holes, and the three sampling holes are communicated with the culture dish body. The three sampling tubes are annularly arranged along the center of the culture dish cover. According to the method, the natural enrichment phenomenon is utilized, the sampling pipe and the sample leaking hopper are matched, water drops containing the nematodes are collected through gravity, compared with a traditional filtering centrifugal collection method, the traditional time-consuming filtering step is omitted, the nematodes can be used after being extracted, the operation process is remarkably simplified, the nematode collection time is greatly shortened, the working efficiency is improved, and the method is suitable for popularization and application. And the three sampling pipes synchronously collect and continuously collect after the sampling pipes are replaced, so that the resource utilization rate is increased.

The invention discloses a pine wood nematode disease rapid detection method, and relates to the field of forestry plant quarantine and pest and disease damage detection, a portable integrated detection device is used for executing the following steps: S1, sample collection: a miniature drilling unit of the device automatically drills into a to-be-detected pine tree trunk to take a wood chip sample; s2, sample pretreatment: conveying and weighing a wood chip sample, transferring the wood chip sample to a crushing and extracting chamber after the wood chip sample reaches a target weight, adding a lysis solution, heating, crushing and extracting to obtain an extracting solution; s3, sample transfer: injecting the extraction liquid into a micro-fluidic chip mechanism by using a diaphragm pump; s4, nucleic acid purification and isothermal amplification: carrying out nucleic acid purification on the extract in a micro-fluidic chip mechanism, and carrying out a nucleic acid isothermal amplification reaction at a constant temperature; and S5, optical detection and result analysis: carrying out real-time fluorescence monitoring on a nucleic acid isothermal amplification process, obtaining and processing a fluorescence signal, calculating the estimated concentration of the pine wood nematodes based on an algorithm model, and evaluating an infection risk level in combination with forest stand background information. The method realizes on-site rapid and accurate detection, and can meet field rapid detection requirements.

The invention relates to the technical field of aviation equipment testing and detection, in particular to an airborne low-scattering radome tool and a positioning device thereof.The airborne low-scattering radome tool comprises a rotating base arranged on the ground, a fixing mechanism is arranged on the rotating base and used for fixedly installing a radome to be detected, and an installing mechanism is further arranged on the rotating base and used for installing the radome to be detected. The mounting mechanism is used for fixedly mounting an antenna, and the antenna on the mounting mechanism can rotate. According to the invention, the fixing mechanism used for installing the antenna housing adopts a non-shielding structural design, the antenna housing is fixed through the arc-shaped cantilever, the core and edge areas of the antenna housing which is integrally curved can be avoided, the electromagnetic exposure rate of the antenna housing reaches 99.8%, and the antenna housing can be effectively protected by arranging the frame made of pine wood and the pointed cone wave-absorbing pad capable of absorbing electromagnetic waves. The influence of scattering and diffraction caused by the tool can be eliminated to the maximum extent, and the accuracy of detection data is ensured.

The utility model relates to the field of pine wood nematode disease, concretely relates to a portable pine wood nematode disease investigation tool box, including tool box body, the main part for depositing investigation instrument, the door, the outside hinged in tool box body, is used for closing tool box body, and the bottom of tool box body all rotatoryly connects with gyro wheel, the mechanical handle is fixedly installed on tool box body, the outside fixed mounting of tool box body has push -and -pull cover. The utility model is provided with tool box body, gyro wheel, mechanical handle, push -and -pull cover, push -and -pull rod, first inner box drawer, second inner box drawer and positioning sliding disc, when using the pine wood nematod disease investigation tool box, can slide first inner box drawer and second inner box drawer through the sliding turning groove and second sliding disc first, then again through the first sliding groove and first sliding disc pull out sliding plate, and the whole first inner box drawer and second inner box drawer are unfolded in the outside of tool box body in this way.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer designed by taking a BXAEP1 gene of Bursaphelenchus xylophilus as a target gene, CRISPR (Clustered An LAMP primer and CRISPR RNA are designed aiming at a BXAEP1 gene of the pine wood nematode as a target gene, a detection kit and a detection method for detecting the pine wood nematode based on an LAMP-CRISPR / Cas12a system are established, specificity and sensitivity tests find that the CRISPR RNA shown in SEQ ID No.8 has the best detection effect, the pine wood nematode can be specifically distinguished from pine wood nematode, and the sensitivity reaches 10 fg / L; the established detection method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of good stability, strong practicability, high amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.

The invention provides a pine wood nematode intelligent detection method based on deep learning. The pine wood nematode intelligent detection method comprises the following steps: acquiring an original microscopic image set by using a microscopic imaging hardware system; performing basic preprocessing, key part identification and labeling and data enhancement operation on the original microscopic image set to obtain a high-quality training sample set; an improved YOLOv11 detection model is constructed; using a YOLOv11 detection model to output candidate boxes and a prediction probability, and screening and positioning the candidate boxes to obtain a double-ROI image and coordinate information; respectively extracting a tail parameter set and a closet parameter set to obtain a unified composite feature vector; and then carrying out online deployment on the YOLOv11 detection model and intuitively displaying a detection result. According to the method, key organs such as vulva caps, joint thorns and tail shapes of the pine wood nematodes can be automatically positioned and recognized under the microscopic imaging condition, and rapid judgment and auxiliary quarantine of pine wood nematode pathogens are achieved.