Patents
Literature
Patsnap Eureka AI that helps you search prior art, draft patents, and assess FTO risks, powered by patent and scientific literature data.

128 results about "Pine wood" patented technology

Pest control management system and method for forest tree breeding

The invention discloses a pest control management system and method for forest tree breeding, and relates to the technical field of forest tree breeding and seedling raising, and the method comprises the following steps: collecting environment data and soil rhizosphere data by collecting multi-mode images of leaves and trunks of a pinus massoniana young forest and videos that pinus massoniana caterpillars gnaw leaves and cluster diffusion; constructing a disease and insect pest recognition model by using a convolutional neural network, performing definite diagnosis on pine wood nematode disease, pine caterpillars and monochamus alternatus hope, and quantifying the disease and insect pest occurrence area and population density of each sub-region; calculating a pine wood nematode disease control demand index, comparing the pine wood nematode disease control demand index with a threshold value, and triggering a corresponding control strategy; the diffusion coefficient of the pine caterpillars is calculated and compared with a risk threshold value, and a prevention and control scheme is formulated; calculating the propagation coefficient of the monochamus alternatus hope, judging the propagation risk and giving a prevention and control strategy; according to the invention, intelligent pest identification, risk quantification and control strategy closed-loop control are realized, treatment and scientific prevention and control are facilitated, and the pest control efficiency of the pinus massoniana young forest and the forest stand health level are improved.
Owner:SICHUAN FORESTRY & GRASSLAND INVESTIGATION & PLANNING INST (SICHUAN FORESTRY & GRASSLAND ECOLOGICAL ENVIRONMENT MONITORING CENT)

Method for treating pine wood nematode infected wood by using poria cocos

The invention relates to the technical field of pest prevention and control, in particular to a method for treating pine wood nematode infected wood through poria cocos. The method comprises the following steps: cutting down current-year epidemic wood, and treating tree heads, tree trunks and boughs to complete peeling and tendon retaining treatment; uniformly cutting the trunk and the boughs into standard cut-logs; the method comprises the following steps: selecting fresh poria cocos sclerotium skin tissues as strains, and cutting according to an area greater than 50% of a cross section; pit pits are dug in the sunny slope; laying small branches on the bottom layer, placing the cut-logs in the pits, fixing the cut-logs with non-woven fabrics after inoculation, covering the upper layer with thin branches and leaves, and covering with soil; the two-stage biological process of mycelium planting, propagation expanding and pine wood nematode and medium insect killing is sequentially completed through a soil heat and moisture preservation environment; the propagation path of pine wood nematodes is blocked through the natural isolation effect of soil, deadwood obtained after poria cocos is subjected to biological verification and then smashed on site to return to forests or be made into fuel particles, and harmless and resource recycling of epidemic wood is achieved. According to the method, the problems of pine wood nematode propagation and epidemic wood treatment are synchronously solved.
Owner:YANTAI FOREST RESOURCES MONITORING & PROTECTION SERVICE CENT (YANTAI COASTAL SHELTERBELT PROVINCIAL NATURE RESERVE MANAGEMENT SERVICE CENT YANTAI FORESTRY SCI INST)

Bacillus tequilensis strain HN-1 and application thereof

The invention discloses a bacillus tequilensis strain HN-1 and application thereof, and belongs to the technical field of biological control of plant parasitic nematodes. The strain HN-1 is identified as bacillus tequilensis, the preservation date is July 28, 2025, the strain HN-1 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20251718, and the preservation number is CCTCC NO: M 20251718. The invention discovers that the bacillus tequilensis strain HN-1 has good insecticidal activity on meloidogyne incognita and pine wood nematode for the first time; the fermentation liquor and fermentation metabolites of the strain HN-1 can be used as a liquid fungicide, or the fermentation liquor is used for preparing a solid fungicide, and the fungicide can be used for preventing and treating meloidogyne incognita and pine wood nematodes in fields.
Owner:YUNNAN UNIV

Multi-source data fusion early warning and precise pesticide application method and system for pine wood nematode disease

This invention discloses a multi-source data fusion early warning and precision application method and system for pine wilt disease, belonging to the field of smart forestry technology. It includes a multi-source data acquisition module, a spatiotemporal data fusion module, a health index calculation module, an external feature extraction module, a dual-channel coupled diagnosis module, a graded early warning module, an application urgency assessment module, an application strategy planning module, and an adaptive optimization module. This invention collects and fuses multi-source data such as satellite remote sensing data, UAV inspection data, ground-based IoT data, and forest meteorological data. It utilizes a dual-channel coupled deep learning model to achieve probabilistic diagnosis of the multidimensional state of individual trees and to extrapolate the spread of the disease. Based on the diagnostic results, it generates graded differentiated application strategies and finally achieves adaptive closed-loop optimization of the calculation parameters and model through a control effect feedback mechanism.
Owner:惠东县森林病虫害防治检疫站

Multi-channel controllable pine wood nematode collection culture dish and culture collection method

The invention relates to a multi-channel controllable pine wood nematode collection culture dish and a culture and collection method, and belongs to the technical field of pine wood nematode culture.The multi-channel controllable pine wood nematode collection culture dish comprises a culture dish body, the culture dish body comprises a culture dish cover and a culture dish body, the culture dish cover is provided with three sampling holes, sampling pipes are installed at the sampling holes, and the three sampling holes are communicated with the culture dish body. The three sampling tubes are annularly arranged along the center of the culture dish cover. According to the method, the natural enrichment phenomenon is utilized, the sampling pipe and the sample leaking hopper are matched, water drops containing the nematodes are collected through gravity, compared with a traditional filtering centrifugal collection method, the traditional time-consuming filtering step is omitted, the nematodes can be used after being extracted, the operation process is remarkably simplified, the nematode collection time is greatly shortened, the working efficiency is improved, and the method is suitable for popularization and application. And the three sampling pipes synchronously collect and continuously collect after the sampling pipes are replaced, so that the resource utilization rate is increased.
Owner:SHANDONG FOREST SCI RES INST

Bursaphelenchus xylophilus disease rapid detection method

The invention discloses a pine wood nematode disease rapid detection method, and relates to the field of forestry plant quarantine and pest and disease damage detection, a portable integrated detection device is used for executing the following steps: S1, sample collection: a miniature drilling unit of the device automatically drills into a to-be-detected pine tree trunk to take a wood chip sample; s2, sample pretreatment: conveying and weighing a wood chip sample, transferring the wood chip sample to a crushing and extracting chamber after the wood chip sample reaches a target weight, adding a lysis solution, heating, crushing and extracting to obtain an extracting solution; s3, sample transfer: injecting the extraction liquid into a micro-fluidic chip mechanism by using a diaphragm pump; s4, nucleic acid purification and isothermal amplification: carrying out nucleic acid purification on the extract in a micro-fluidic chip mechanism, and carrying out a nucleic acid isothermal amplification reaction at a constant temperature; and S5, optical detection and result analysis: carrying out real-time fluorescence monitoring on a nucleic acid isothermal amplification process, obtaining and processing a fluorescence signal, calculating the estimated concentration of the pine wood nematodes based on an algorithm model, and evaluating an infection risk level in combination with forest stand background information. The method realizes on-site rapid and accurate detection, and can meet field rapid detection requirements.
Owner:JIANGXI ACAD OF FORESTRY

A portable bursaphelenchus investigation kit

ActiveCN224374051UWork tools storageBursaphelenchusAgricultural engineering
The utility model relates to the field of pine wood nematode disease, concretely relates to a portable pine wood nematode disease investigation tool box, including tool box body, the main part for depositing investigation instrument, the door, the outside hinged in tool box body, is used for closing tool box body, and the bottom of tool box body all rotatoryly connects with gyro wheel, the mechanical handle is fixedly installed on tool box body, the outside fixed mounting of tool box body has push -and -pull cover. The utility model is provided with tool box body, gyro wheel, mechanical handle, push -and -pull cover, push -and -pull rod, first inner box drawer, second inner box drawer and positioning sliding disc, when using the pine wood nematod disease investigation tool box, can slide first inner box drawer and second inner box drawer through the sliding turning groove and second sliding disc first, then again through the first sliding groove and first sliding disc pull out sliding plate, and the whole first inner box drawer and second inner box drawer are unfolded in the outside of tool box body in this way.
Owner:GUANGXI FORESTRY GRP GUIJIANG FORESTRY SURVEY & DESIGN CO LTD

LAMP primer designed by taking BXAEP1 gene of bursaphelenchus xylophilus as target gene, CRISPR RNA and application thereof

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer designed by taking a BXAEP1 gene of Bursaphelenchus xylophilus as a target gene, CRISPR (Clustered An LAMP primer and CRISPR RNA are designed aiming at a BXAEP1 gene of the pine wood nematode as a target gene, a detection kit and a detection method for detecting the pine wood nematode based on an LAMP-CRISPR / Cas12a system are established, specificity and sensitivity tests find that the CRISPR RNA shown in SEQ ID No.8 has the best detection effect, the pine wood nematode can be specifically distinguished from pine wood nematode, and the sensitivity reaches 10 fg / L; the established detection method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of good stability, strong practicability, high amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

Intelligent pine wood nematode detection method based on deep learning

The invention provides a pine wood nematode intelligent detection method based on deep learning. The pine wood nematode intelligent detection method comprises the following steps: acquiring an original microscopic image set by using a microscopic imaging hardware system; performing basic preprocessing, key part identification and labeling and data enhancement operation on the original microscopic image set to obtain a high-quality training sample set; an improved YOLOv11 detection model is constructed; using a YOLOv11 detection model to output candidate boxes and a prediction probability, and screening and positioning the candidate boxes to obtain a double-ROI image and coordinate information; respectively extracting a tail parameter set and a closet parameter set to obtain a unified composite feature vector; and then carrying out online deployment on the YOLOv11 detection model and intuitively displaying a detection result. According to the method, key organs such as vulva caps, joint thorns and tail shapes of the pine wood nematodes can be automatically positioned and recognized under the microscopic imaging condition, and rapid judgment and auxiliary quarantine of pine wood nematode pathogens are achieved.
Owner:JILIN TEACHERS INST OF ENG & TECH

LAMP primer designed by taking BXPC1 gene of bursaphelenchus xylophilus as target gene, CRISPR RNA and application thereof

The invention discloses an LAMP primer designed by taking a BXPC1 gene of bursaphelenchus xylophilus as a target gene, CRISPR RNA and application of the LAMP primer. The invention designs an LAMP (loop-mediated isothermal amplification) primer and CRISPR (clustered regularly interspaced short palindromic repeats) RNA (ribonucleic acid) by aiming at a BXPC1 gene of the pine wood nematodes as a target gene and establishes a detection kit and a detection method for detecting the pine wood nematodes based on an LAMP-CRISPR / Cas12a system. Specificity and sensitivity tests show that the CRISPR RNA as shown in SEQ ID No.8 has the best detection effect, pine wood nematode and bursaphelenchus mucronatus can be specifically distinguished, and the sensitivity reaches 0.1 pg / L; the method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of being good in stability, high in practicability, high in amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

A genetic control method for monochamus alternatus

PendingCN122168456ABiocideBacteriaAdult stagePesticide residue
The application provides a method for preventing and treating monochamus alternatus, which comprises putting male monochamus alternatus carrying Wolbachia into a target prevention and treatment area of monochamus alternatus not carrying Wolbachia. The method has low investment cost, is green, safe, environment-friendly, has no pesticide residue and high risk, conforms to the green prevention and control principle and needs of diseases and pests in the new era, is less affected by external temperature, humidity and other climate environments, is suitable for wide plots, is a new prevention and treatment method, is early in the prevention and treatment period, prevents and treats from the egg stage, reduces the double hazards of the larval stage dry woodworm and the pupal stage adult stage spreading pine wood nematode disease from the root, has good prevention and treatment effect, and can significantly reduce the egg hatching rate of monochamus alternatus by more than 90%.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI +1

Fixing clamp device for poria cocos fungus bag planting

ActiveCN224267639UImprove planting effectpromote reproductionAgriculture gas emission reductionCultivating equipmentsMyceliumEngineering
The utility model is applicable to the technical field of poria cocos fungus bag planting, and provides a fixing clamp device for poria cocos fungus bag planting, which comprises a connecting frame, and mounting plates are symmetrically and fixedly mounted on the connecting frame; the clamping ring is assembled on the mounting plate, a clamping mechanism is arranged between the clamping ring and the mounting plate, and the clamping mechanism is used for driving the clamping ring to move to clamp pine sections used for poria cocos fungus bag planting; the fixing band is assembled on the mounting plate and is used for fixing the poria cocos fungus bag on a pine section; positioning columns are arranged on the two mounting plates, connecting rings are fixedly mounted at the two ends of the fixing belt, and the two connecting rings are connected with the two positioning columns in a matched mode. According to the fixing clamp device for poria cocos fungus bag planting, poria cocos fungus bags and pine sections can be clamped and positioned, the fitting degree of the poria cocos fungus bags and the pine sections is enhanced, hyphae can better breed on the pine, and the poria cocos planting effect is improved.
Owner:SOUTHWEST FORESTRY UNIVERSITY +1

Preparation of amide compound containing benzoheterocycle and application of amide compound as nematicide

The invention relates to preparation and application of a series of benzoheterocycle-containing amide compounds with novel structures. The structural general formulas of the series of the amide compounds containing the benzoheterocycles are shown as a formula (I) and a formula (II), and research finds that the amide compounds containing the benzoheterocycles have excellent poisoning activity on pine wood nematodes which are major forest-devastating plant parasitic nematodes. The compound can be used as a nematicide candidate for research and development of a novel nematicide with high efficiency, low toxicity and environmental protection.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Use of a cyclopropane compound for preventing and treating forestry pests

The application discloses a preparation method of a cyclopropane compound and application of the cyclopropane compound in nematode killing activity. The application uses an electrochemical method to quickly cyclopropanate a methylene compound by using a cheap and easily available sulfide. The synthesis method is simple, clean, efficient, short in reaction time, low in energy consumption, and cheap and easily available in raw materials, and is suitable for mass production and better research. Through activity testing, the cyclopropane compound has strong activity against pine wood nematode disease, and relevant research provides a good basis for strong biological activity, anticancer activity, antibacterial activity and anti-inflammatory activity.
Owner:NANJING FORESTRY UNIV

Molecular marker related to slash pine wood property, DNA fragment, kit and application thereof

The invention discloses a molecular marker related to slash pine wood property, a DNA (deoxyribonucleic acid) fragment, a kit and application of the molecular marker, the SNP molecular marker is scaffold 18550859010 and is located at the 59010th site of the fragment scaffold 185508; the polymorphism of the SNP sites is C / T, the genotype of the SNP site of the slash pine with high wood density is CC, and the genotype of the SNP site of the slash pine with low wood density is CT; the nucleotide sequence of the DNA fragment related to the slash pine wood property is as shown in SEQ ID NO. 1 or SEQ ID NO. 2; comprising a forward primer and a reverse primer which are used for amplifying a DNA fragment related to slash pine wood property; the nucleotide sequence of the forward primer is as shown in SEQ ID NO.3, and the nucleotide sequence of the reverse primer is as shown in SEQ ID NO.4; the molecular marker, the DNA fragment and the kit are used for slash pine wood property-related phenotype identification, early prediction, breeding and the like, and the problems that existing slash pine wood property-related traits are late in identification, long in breeding period, low in breeding efficiency and the like can be solved.
Owner:RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY

A composition for preventing and treating pine wood nematode and application thereof

The application discloses a composition for preventing and treating Bursaphelenchus xylophilus and application thereof, and belongs to the technical field of biological pesticides. The composition is compounded by taking a crude extract of Zanthoxylum bungeanum as a main active ingredient and a crude extract of Astragalus membranaceus as a synergistic ingredient. The composition has high Bursaphelenchus xylophilus killing activity. After Bursaphelenchus xylophilus is treated by 600ug / mL of the composition solution for 24 hours, the corrected mortality of the Bursaphelenchus xylophilus reaches 99.33%. The composition can effectively prevent the occurrence of the pine wilt disease in a forest, and the control effect reaches 79.59%. In addition, the preparation process of the composition is simple, the composition is easy to use, and the composition is suitable for popularization and use.
Owner:ANHUI SENKE BIOTECHNOLOGY CO LTD

A method for detecting pine wood nematode disease of a drone based on multi-modal features

The present application relates to a kind of unmanned aerial vehicle pine wood nematode disease detection method based on multi-modal feature, by constructing image-text multi-modal dataset to provide prior semantic knowledge;Adopt vegetation index threshold mask filtering (VTMF) to enhance spectral difference and inhibit noise;Utilize the W-KAN module of fusion wavelet transform and KAN attention to carry out frequency domain denoising and feature reservation;Through four-domain diffusion graph convolution residual attention module (Q-DGCRAB) modeling long-distance spatial dependence relationship;Introduce epidemic semantic enhancement detection module (ESADM) to realize text-visual feature fusion;And adopt forest guardian collaborative optimization algorithm (FGA-CE) dynamic optimization training process;The present application solves the problem of low pine wood nematode disease detection precision caused by complex background, texture repetition, significant noise and insufficient semantic information in high-altitude unmanned aerial vehicle image, significantly improves the detection precision and robustness of pine wood nematode disease in complex stand environment, realizes early accurate, real-time monitoring of pine wood nematode disease.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

Device for monitoring and killing of pine wood nematode in forestry

ActiveCN224402685UTrappingHusk
The utility model discloses a forestry bursaphelenchus xylophilus's monitoring and killing device relates to pest protection technical field. The utility model discloses a shell and the rectangular groove on the shell outer wall of setting up a plurality, still include: monitoring part, monitoring part installs on the shell, cleaning part, the cleaning part sets up in the shell and extends to the shell outside and lock catch part, lock catch part sets up on the shell, wherein, a plurality of rectangular grooves are in the shell and are in the circular array distribution, the monitoring part includes the support frame fixedly connected on the shell outer wall, the bottom fixed connection of support frame has two monitoring, the bottom inner wall of shell places and has collected the box. The utility model discloses, through setting up the cleaning part, solved the current bursaphelenchus xylophilus killing device when using, inconveniently clean device inner wall, in the running process, the inner wall will attach the insect body, impurity etc, the device is difficult to keep clean for a long time, thereby influence the problem of the stability of the function of trapping and monitoring.
Owner:CHONGQING HA HA JING PEST CONTROL CO LTD

A pine wood nematode collecting device

The utility model relates to the technical field of insect collecting equipment, concretely relates to a pine wood nematode collecting device, including base, the base top fixedly connected with insect collecting mechanism, the base bottom is provided with fixed subassembly, among them: The fixed subassembly includes two arc connecting plates, two The arc connecting plates pass through opposite or opposite movement adjustment distance between two arc connecting plates, can with different specifications connecting piece steady clamping between two arc connecting plates, adapt to different diameter connecting piece, expand the applicable scene of device, one end of fixed subassembly is provided with adjusting assembly, through Adjusting assembly rotation drive fixed subassembly one end rotation adjusts the angle of fixed subassembly, satisfy fixed subassembly with different direction connecting piece's connection, increase the adaptability of insect collecting mechanism installable position, effectively guarantee the insect collecting mechanism in the moth module can always be oriented optimal monitoring direction, improve the insect collecting efficiency.
Owner:JILIN PROVINCIAL ACADEMY OF FORESTRY SCIENCES JILIN

Composite adsorption material applied to CO2 gas-liquid separation and preparation method thereof

The invention relates to a composite adsorption material applied to CO2 gas-liquid separation and a preparation method of the composite adsorption material, and belongs to the technical field of chemical material preparation, a three-layer structure is constructed by taking oriented carbonized pine wood as a supporting framework, taking rare earth / nano-particle dual-modified zeolite as an adsorption core and taking a hydrophobic coating as a protective layer; the preparation method comprises the continuous processes of pine gradient carbonization, zeolite dual modification, in-situ coating forming and continuous sintering. The material solves the problems of capacity attenuation, high regeneration energy consumption and microbial pollution of a traditional adsorption material under a high-humidity working condition, is high in CO2 saturated adsorption capacity and high in capacity retention rate after regeneration, is adaptive to scenes such as a transcritical CO2 heat pump, high-humidity industrial waste gas purification and high-salinity wastewater treatment, can realize low-energy-consumption regeneration through photocatalysis, and is remarkable in effect.
Owner:HENAN HAOLI INTELLIGENT TECH CO LTD

Bacillus safensis HMQAU23223 and application thereof

The invention relates to the field of biocontrol of plant diseases. The invention provides a bacillus safensis strain HMQAU23223, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 13, 2025, and the preservation registration number is CGMCC No.36623. The bacillus safensis strain HMQAU23223 has the advantages that the preservation number is CGMCC No.36623; the invention also provides a biocontrol inoculant. The invention also provides a preparation method of the biocontrol inoculant. The invention further provides the application of the bacillus safensis strain HMQAU23223 or the biocontrol microbial inoculum or the biocontrol microbial inoculum prepared by the preparation method. The invention further provides the application of the bacillus safensis strain HMQAU23223 or the biocontrol microbial inoculum prepared by the preparation method. The invention further provides a method for preventing and treating plant diseases. The invention also provides an application of the bacillus safensis strain HMQAU23223 in preparation of a compound for inhibiting phytopathogen or preventing and treating plant pathogenic nematodes. The fermentation filtrate of the bacillus safensis strain HMQAU23223 provided by the invention has the lethal effects of 89% or above on pine wood nematode, meloidogyne incognita, wheat cyst nematode, heterodera filipjevi and ditylenchus destructor, and has better biocontrol potential.
Owner:QINGDAO AGRI UNIV

Application of natural unsaturated aldehyde in prevention and treatment of plant parasitic nematodes

The application discloses application of natural unsaturated aliphatic aldehyde in prevention and treatment of plant parasitic nematodes. The natural unsaturated aliphatic aldehyde is trans, trans-2,4-heptadienal and trans-2-heptenal. The plant parasitic nematodes are root-knot nematodes, cyst nematodes and pine wood nematodes. Both the trans, trans-2,4-heptadienal and the trans-2-heptenal can exhibit excellent nematocidal activity through contact killing and fumigation, which provides a basis for development of green and environmentally-friendly nematocidal agents, and has a good application prospect for replacing traditional chemical nematocidal agents.
Owner:CHONGQING UNIV

Monochamus alternatus adult micro-capsule slow-release synergistic trapping and killing preparation and prevention and control method

The invention belongs to the technical field of green prevention and control of forestry pests, and discloses a microcapsule slow-release synergistic trapping and killing preparation for adult monochamus alternatus and a prevention and control method. According to the preparation, the monochamus alternatus attractant and the 6% abamectin chlorobenzoyl microcapsule suspending agent are embedded in a polymer capsule wall material, and synchronous slow release of the monochamus alternatus attractant and the 6% abamectin chlorobenzoyl microcapsule According to the prevention and control method, an unmanned aerial vehicle or an airplane is adopted for precisely applying pesticide in a strip mode, and monochamus alternatus adults are efficiently prevented and controlled through a directional luring-concentrated gathering-targeted killing mechanism. The pesticide dosage is only 1 / 4 of that of conventional prevention and control, the comprehensive cost is remarkably reduced, the prevention and control effect is not statistically different from that of conventional agents, an ecological sensitive area can be flexibly avoided, high efficiency, economical efficiency and ecological friendliness are achieved, and a key technical scheme is provided for prevention and control of pine wood nematode disease vector insects.
Owner:HENAN YEHUA BIOTECHNOLOGY CO LTD +1

Method for enhancing pine wood nematode disease resistance of larch through jasmonic acid signal mediated flavonoid biosynthesis

The invention discloses a method for enhancing pine wood nematode disease resistance of larch through jasmonic acid signal mediated flavonoid biosynthesis. The method comprises the following steps that 1, the larch is inoculated with pine wood nematode; 2, performing high-throughput omics sequencing sampling and data analysis; 3, treating larch seedlings with methyl jasmonate; 4, the inhibition effect of the kaempferol and the citrus aurantium on the pine wood nematodes is obtained. According to the method, larch is taken as a research object, a disease stress system is constructed by artificially inoculating pine wood nematodes, leaf / stem tissues at different time points after infection are selected, and sequential multiomics analysis is carried out by combining transcriptome sequencing and a wide targeting metabolome technology; the theoretical basis and method for improving the pine wood nematode disease resistance of larch by regulating and controlling the biosynthesis of kaempferol and citrus aurantium through a jasmonic acid signal way are defined.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

Method for preventing and controlling pine wood nematode disease by killing vector insects based on RNA interference technology and application thereof

PendingCN122445640ABiotechnologyNematode
The application belongs to the technical field of biological prevention and treatment of forestry diseases and insect pests, and discloses a method for preventing and controlling pine wood nematode disease by killing medium insects based on RNA interference technology and application thereof. The method screens RNA interference lethal targets from Monochamus alternatus, and the targets are selected from at least one of IAP genes and RPL40 genes; RNA interference molecules targeting the targets are prepared, and are prepared into RNAi nucleic acid preparations together with nucleic acid loading materials, nucleic acid protection materials, plant surface adhesion spreading materials, light stable protection materials and aqueous media; and the preparations are applied to Monochamus alternatus, pine branches, pine needles, tree barks and feeding, activity, habitat or egg laying sites thereof. The method can improve the stability, retention and delivery efficiency of RNA interference molecules in a forest environment, induce Monochamus alternatus to reduce the expression of lethal targets and kill medium insects, thereby reducing the transmission risk of pine wood nematodes, and is suitable for green prevention and control of pine wood nematode disease.
Owner:INDIGO (BEIJING) BIOTECHNOLOGY RESEARCH CO LTD

Pine wood nematode disease monitoring sampler

The utility model relates to forestry disease and insect pest monitoring equipment technical field, concretely for pine wood nematode disease monitoring sampler, include: support seat, first waist type piece, second waist type piece and third waist type piece, both ends of support seat all respectively have clamping arm and prop open arm, and two prop open arms are located between two clamping arms position, first waist type piece, second waist type piece and third waist type piece are in turn arranged from top to bottom. The utility model drives clamping arm and support seat to occur hinged through movable element cooperation third hinged link and first hinged link, and make two clamping arms gradually relatively rotate, and the tree is steadily clamped, and the movable column drives prop open arm and support seat hinged through second hinged link simultaneously, makes the tree gap expand, when the pine nematode exposes, keeps the gap expansion state, at this moment, the pine nematode in the gap can be taken out from the forceps, to realize the single person convenient pine nematode and reduce the purpose of the damage to the tree trunk.
Owner:JILIN PROVINCIAL ACADEMY OF FORESTRY SCIENCES JILIN

Isaria fumosorosea and its application in preventing and treating pine wood nematode disease

This invention discloses a fungal encapsulation agent for *Ischemicula elais* and its application in the control of pine wilt disease, belonging to the field of microbial control technology. This invention uses *Ischemicula elais* as the functional microorganism, combined with carrier materials, additives, and active ingredients to prepare a solid microbial encapsulation agent. The shelf life of *Ischemicula elais* spores at room temperature is extended from 2-3 days for traditional spore solutions to over 3-6 months, and up to 12 months under 4°C refrigeration, solving the problem of inconvenient low-temperature storage in mountainous areas. The microencapsulation components form a slow-release protective layer, preventing activity loss caused by environmental factors such as high temperature and drought.
Owner:LINYI UNIVERSITY

Bursaphelenchus xylophilus disease identification method and system based on unmanned aerial vehicle image

The invention relates to the technical field of remote sensing monitoring, in particular to a pine wood nematode disease identification method and system based on an unmanned aerial vehicle image, and the method comprises the following steps: obtaining a pine forest multispectral image through an unmanned aerial vehicle, extracting edge pixels through a Canny algorithm, decomposing a direction to generate a sequence, calculating a gradient offset, counting curvature mutation points, and extracting curvature characteristic parameters. The method comprises the following steps: removing abnormal edge segments, performing DBSCAN clustering to obtain candidate areas, constructing a radial channel to collect gray values, generating gray attenuation features, extracting a three-dimensional feature space, and performing K-means clustering to output a recognition result graph. According to the method, multi-spectral image gradient decomposition is combined with curvature fluctuation characteristic analysis, disease spot microscopic distortion is accurately captured, non-pathological interference is inhibited through curvature mutation points and density clustering, three-dimensional characteristics are constructed through a radial gray attenuation main trend, gray identification is enhanced through direction offset analysis, experience dependence is reduced through multi-threshold screening, and the method is suitable for large-scale popularization and application. The dynamic self-matching framework improves the robustness of forest region detection, and realizes the autonomous recognition of disease spots.
Owner:GUANGZHOU INST OF FORESTRY & LANDSCAPE ARCHITECTURE

Selective nematicidal compound I based on bursaphelenchus xylophilus neuropeptide receptor and application thereof

The invention discloses a selective nematicidal compound I based on a bursaphelenchus xylophilus neuropeptide receptor and application of the selective nematicidal compound I. The invention relates to a small molecular lead compound for killing bursaphelenchus xylophilus and application of the small molecular lead compound, the chemical formula of the small molecular lead compound is C25H27 Cl N2 O3 S, and the median lethal concentration of the small molecular lead compound for killing the bursaphelenchus xylophilus is 9.30 mg / L. The micromolecular lead compound can effectively inhibit the feeding capacity and population number of pine wood nematodes, and shows good nematode killing potential. The small molecular lead compound has a good application prospect in the aspect of preventing and treating pine wood nematode diseases.
Owner:ZHEJIANG FORESTRY UNIVERSITY