121 results about "Biological process" patented technology

The invention relates to the technical field of biological process intelligent control, and discloses a pomegranate leaf fermentation process optimization and intelligent control system based on digital twinning. According to the system, the Internet of Things and a visual sensor are integrated through a multi-source data sensing module, so that synchronous acquisition and fusion of temperature, pH, dissolved oxygen, flora density and image data are realized; the intelligent data preprocessing module extracts high-quality features by adopting a self-adaptive filtering technology; the digital twin simulation engine module drives the virtual model to dynamically update based on real-time data; the deep prediction module accurately predicts the fermentation trend through a deep learning algorithm; the intelligent diagnosis module realizes abnormal root cause analysis by using a knowledge graph; the reinforcement learning optimization module autonomously generates an optimal control strategy based on the diagnosis result; and the self-adaptive execution module accurately regulates and controls fermentation parameters. According to the system, multi-dimensional perception, high-precision prediction and adaptive optimization control of the fermentation process are realized, and the accuracy and stability of process control are effectively improved.

A computer-implemented method is provided for monitoring and / or controlling a chemical and / or biological process. The method comprises: obtaining (S10) a time series dataset comprising observations, each one of the observations being collected with respect to the chemical and / or biological process at a particular time point, wherein each one of the observations includes values of observed parameters obtained with a spectroscopic method at the particular time point and a value of an analyte parameter obtained with a reference measurement method at the particular time point; obtaining (S20) a prediction model for estimating a predicted value of the analyte parameter in the chemical and / or biological process, the prediction model being trained using at least part of the time series dataset; validating (S30) the prediction model by assessing an ability of the prediction model to predict a difference between an actual value of the analyte parameter which would be obtained with the reference measurement method at a given time point and an expected value of the analyte parameter at the given time point, using a set of observations that is not included in the at least part of the time series dataset used for training the prediction model; determining (S40) whether the prediction model is valid or invalid based on a result of the validating; and monitoring and / or controlling (S50) the chemical and / or biological process when the prediction model is determined to be valid.

The invention discloses a Halomonas alkaliphila NCSL-JXB2 which is a flocculating bacterium, and the preservation number of the Halomonas alkaliphila NCSL-JXB2 is CGMCC (China General Microbiological Culture Collection Center) No.33827. The invention further discloses a preparation method of the Halomonas alkaliphila NCSL-JXB2. The invention also discloses a culture method and application of the basophilic halomonas. The flocculating bacterium Halomonas alkaliphila NCSL-JXB2 disclosed by the invention can tolerate an ultra-high salt environment, is wide in salt tolerance range, can survive in an environment with the salinity of 0-25wt%, and has a good flocculation effect, and the self-flocculation rate is 66.3%. The strain can be used as a biological enhancement means to assist other biological processes, or is added together with other functional strains, or is added independently to accelerate the process starting speed, improve the operation effect of the biological process, improve the treatment efficiency of high-salt organic wastewater and reduce the operation cost.

The invention discloses a protein complex identification method based on knowledge enhancement graph comparative learning, and belongs to the technical field of bioinformatics. Edge trimming is performed by using a gene expression profile and subcellular localization data, so that a knowledge enhancement PPI network is constructed; and incorporating the molecular function and the biological process as network attributes as attributes for enhancing the network. Graph structure enhancement is applied to knowledge enhanced PPI attribute networks, and multiple perturbation views are generated with diverse random perturbation policies including edge addition, edge discarding, and feature masking to help mitigate data sparsity. A perturbation view generated by graph structure enhancement is coded through a randomized graph convolutional network layer, so that multi-scale features are extracted, key PPI interaction is reserved, and meanwhile prediction performance is improved. And finally, the original PPI network is weighted again, and the protein complex is identified by adopting a core-affiliated strategy.

The invention relates to the technical field of pest prevention and control, in particular to a method for treating pine wood nematode infected wood through poria cocos. The method comprises the following steps: cutting down current-year epidemic wood, and treating tree heads, tree trunks and boughs to complete peeling and tendon retaining treatment; uniformly cutting the trunk and the boughs into standard cut-logs; the method comprises the following steps: selecting fresh poria cocos sclerotium skin tissues as strains, and cutting according to an area greater than 50% of a cross section; pit pits are dug in the sunny slope; laying small branches on the bottom layer, placing the cut-logs in the pits, fixing the cut-logs with non-woven fabrics after inoculation, covering the upper layer with thin branches and leaves, and covering with soil; the two-stage biological process of mycelium planting, propagation expanding and pine wood nematode and medium insect killing is sequentially completed through a soil heat and moisture preservation environment; the propagation path of pine wood nematodes is blocked through the natural isolation effect of soil, deadwood obtained after poria cocos is subjected to biological verification and then smashed on site to return to forests or be made into fuel particles, and harmless and resource recycling of epidemic wood is achieved. According to the method, the problems of pine wood nematode propagation and epidemic wood treatment are synchronously solved.

The invention discloses a multi-layer heterogeneous network unicellular organism network inference method based on meta-path enhancement, which mainly comprises a gene regulation knowledge base enhanced multi-layer heterogeneous network construction module for integrating an external gene interaction network and multiple omics data such as scRNA-seq, scATAC-seq, ST and the like; constructing a single-cell multi-omics multilayer heterogeneous network containing cell-cell, cell-gene and gene-gene relationships, and fusing spatial constraints to consider cell positions and tissue structures; and the feature enhancement module based on the meta-path explores complex semantics of the network by designing a multi-hop meta-path mode, designs an adaptive multi-view learning framework and a multi-round enhancement mechanism, and optimizes feature representation by using cell-gene interaction and cross-modal attention fusion. The unicellular biological network can be effectively deduced, the deduction accuracy and biological interpretation are remarkably improved, the method plays an important role in understanding the cell biological process, developing and treating diseases and the like, has good expandability, and can further integrate multi-modal omics data such as proteomics and metabonomics.

A first unit (1) for treating a biological process liquid comprises a first side face (2), a second side face (3) and a front face (4) meeting the two side faces. The front face comprises: a plurality of valves (7) adapted to receive one or more legs (8) of the disposable flow path (6) and to act on the one or more legs (8) of the disposable flow path (6); optionally, one or more pumps (10) adapted to receive the one or more legs of the disposable flow path and to act on the one or more legs of the disposable flow path; optionally, one or more sensors (11) adapted to receive and measure one or more parameters in one or more legs of the disposable flow path; wherein the plurality of valves and optional pumps and sensors are vertically offset from each other to give a slope of at least 3.0 degrees from a horizontal plane (h) to one or more legs of the disposable flow path received by the valves and optional pumps and sensors.

Biological process for treating liquid effluent using the macrofungus Lentinus. The present invention describes a process for treating tannery and electroplating effluents contaminated by different types of metals (aluminum, copper, chromium, iron, and nickel) by adding biomass of a macrofungus from the Lentinus crinitus complex to the treatment process. This invention falls within the fields of biotechnology, biology, and environmental engineering.

This invention discloses methanogenic lyases with the same protein family annotation as PeiR lyases and their applications. These enzymes are obtained through homology screening based on the known characteristics of PeiR lyases targeting peptide bonds in the methanogenic cell wall, using the Pfam protein family database annotation. PeiR lyase proteins participate in the biological process of hydrolyzing archaea cell walls, effectively killing methanogens and reducing methane production. Proteins homologous to this protein have potential methane-reducing potential. This invention integrates rumen microbial metagenomic sequencing data, uses a series of bioinformatics software to screen methanogenic viral proteins, and combines Pfam functional domain annotation for homology analysis, ultimately identifying a series of lyases. These lyases were successfully expressed in a prokaryotic expression system, and in vitro gas production experiments also showed that the crude enzyme solution significantly reduced methane production.

This application relates to a method, apparatus, computer device, and storage medium for identifying enzyme substrates. The method for identifying enzyme substrates analyzes the sequence information of substrate peptides before and after enzyme digestion by quantitative detection of control samples and enzyme inhibitor-treated samples, thereby identifying potential enzyme substrates. It effectively identifies enzyme cleavage sites, detects the sequences of substrate peptides before and after enzyme digestion, and promotes enzyme substrate identification and site analysis. This plays an important role in understanding biological processes and identifying biomarkers for diseases.

This invention discloses a quantitative method and system for the contribution of river nitrous oxide sources and fate processes, relating to the field of biotechnology. The method includes: collecting surface water and groundwater samples; determining the physicochemical properties of the water samples, including calculating and quantifying the water-to-groundwater exchange ratio; extracting dissolved nitrous oxide from the water samples after determining the physicochemical properties and performing isotope analysis on the nitrous oxide to generate isotope test results; conducting indoor culture experiments on the water samples after completing the isotope analysis to determine whether there are abiotic sources of nitrous oxide in the water samples, generating indoor culture results; and quantifying the contribution of river nitrous oxide sources based on the results of the water-to-groundwater exchange ratio, the isotope test results, and the indoor culture results. This invention effectively analyzes the sources and fate processes of nitrous oxide in rivers and accurately quantifies the contributions of different sources.

The invention discloses a collagen peptide composition with a function of increasing muscle strength and application of the collagen peptide composition. The collagen peptide composition comprises fish collagen peptide and beef tendon collagen peptide. A mouse senescence sarcopenia model is constructed, then a collagen peptide composition is used for intragastric intervention, and the improvement effect is evaluated through behavioral testing, body component analysis and HE staining. The potential target and regulatory pathway of the collagen peptide composition are explored by combining network pharmacology and molecular docking. Results show that the four-limb grip strength, treadmill exhaustion time and movement distance of a model mouse can be remarkably improved, quadriceps femoris muscle and gastrocnemius muscle indexes are recovered, and muscle fiber atrophy and collagen deposition are relieved. And it is found that TNF, IL1B, AKT1, mTOR and MAPK1 may be core targets of the TNF, IL1B, AKT1, mTOR and MAPK1. The polypeptide plays a role by regulating and controlling biological processes such as MAPK cascade, protein processing and inflammatory reaction, cell apoptosis, a cAMP pathway and a calcium signal pathway.

This invention discloses a methanogenic lyase homologous to PeiR lyase and its applications. The PeiR lyase protein, targeting the peptide bonds of the methanogenic cell wall, participates in the biological process of hydrolyzing the archaea cell wall, effectively killing methanogens and reducing methane production. Proteins homologous to this lyase possess potential methane-reducing potential. This invention utilizes a series of bioinformatics software to identify protein sequences encoded by methanogenic viruses from a large amount of rumen microbial virome sequencing data, and analyzes the structural homology between these proteins and PeiR. Ultimately, this invention successfully obtained a series of methanogenic lyases homologous to PeiR. To achieve the expression of these lyases, this invention designed multiple primers to synthesize the target sequences and successfully expressed them in a prokaryotic expression system. In vitro gas production experiments with the crude enzyme solution showed that these lyases are effective in reducing methane production.

The invention relates to the technical field of microbial carbon sequestration, and discloses bacillus megaterium, a carbon sequestration microbial agent as well as a preparation method and application of the carbon sequestration microbial agent. The preservation number of the bacillus megaterium provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.35586. The bacillus megaterium provided by the invention is a new strain of bacillus megaterium separated from a water sample in an oil field. The bacillus megaterium can effectively immobilize carbon dioxide in the atmosphere and generate gases such as nitrogen under a high-temperature condition through a unique metabolic pathway, so that the synergistic effect of carbon sequestration and gas production is realized, and the energy recovery rate and environmental benefits of the whole biological process are improved.

This invention belongs to the field of synthetic biology and microbial engineering, and discloses a chemical-biological method for preparing the isopentenyl bibenzyl compound 2-isopentenyl-3,3',4',5-tetrahydroxybibenzyl (NPB-1575) which has anti-ischemic stroke activity. The specific disclosed method includes: (1) chemically synthesizing the substrate dihydropiperidine; (2) constructing an engineered Escherichia coli strain based on the isopentenyl utilization pathway, co-expressing hydroxyethylthiazol kinase, isopentenyl phosphokinase, isopentenyl pyrophosphate isomerase and specific isopentenyl transferase, and efficiently synthesizing the isopentenyl donor; (3) using this strain to catalyze dihydropiperidine in whole cells, the yield in a 200L fermenter can reach 2.54g / L, which is nearly 40 times higher than the existing method; (4) obtaining NPB-1575 with a purity >98.5% by macroporous adsorption resin and normal / reversed phase chromatography and recrystallization. This method uses readily available raw materials, involves simple steps, is environmentally friendly, and yields high purity, laying the foundation for the development of NPB-1575 as a new drug for ischemic stroke and showing significant application potential.

The present invention relates to a flexible, scalable up-stream pharmaceutical product Process System for expression of Mabs and mixing for mRNA bases consisting of multiple process containers, a liquid dividing manifold, a fluid conveying device, a solids separation unit.

The invention relates to the technical field of biological medicines, and particularly provides a hair follicle regeneration nano activator as well as a preparation method and application thereof. The activator comprises a first coupling monomer and a second coupling monomer, an oligonucleotide sequence of the first coupling monomer and an oligonucleotide sequence of the second coupling monomer are at least partially complementary and form a double-chain structure through base pairing, the first coupling monomer is formed by coupling a first polypeptide and a first ssDNA, and the second coupling monomer is formed by coupling a second polypeptide and a second ssDNA. The second coupling monomer is formed by coupling a second polypeptide and a second ssDNA; wherein the first polypeptide and the second polypeptide are both specifically targeted to beta-catenin protein. The polypeptide-DNA-polypeptide dimer has a polypeptide-DNA-polypeptide sandwich-type dimer structure, can be specifically combined with beta-catenin in stem cells, only regulates and controls a Wnt / beta-catenin pathway, does not generate non-specific influence on other pathways, and can efficiently activate a hair follicle regeneration related signal pathway and promote a hair follicle development biological process.

The invention discloses a PPR (pentatricopeptide repeats) high-salt-resistant all-potent nuclease mutant and application thereof. The invention firstly discloses an amino acid sequence of the PPR high-salt-resistant all-potent nuclease mutant as shown in SEQ ID NO. 1. The invention further discloses application of the PPR high-salt-resistant all-potent nuclease mutant to removal of nucleic acid. According to the PPR high-salt-resistant totipotent nuclease mutant, alanine at the 165 site of wild PPR high-salt-resistant totipotent nuclease which does not contain signal peptide is replaced with aspartic acid, overexpression can be carried out in a prokaryotic host, and a large number of proteins can be obtained through purification. The PPR high-salt-resistant all-purpose nuclease mutant disclosed by the invention maintains high activity in a wide salt concentration range, a temperature range, a pH range and a Mg < 2 + > range, and can be compatible with most additives used in a biological process. In addition, the activity of the PPR high-salt-resistant all-purpose nuclease mutant is superior to that of other domestic competitive products, and the competitive product level with the best commercial effect at present can be achieved.

The application discloses a RAU risk generation and prediction method and system based on a causal guided diffusion model, belongs to the cross technical field of medical information technology and artificial intelligence, and does not directly predict a risk label, but learns and simulates complete evolution distribution from a current health state to multiple possible health states in the future. Through Monte Carlo sampling, the application can generate a large number of 'future selves' conforming to individual characteristics, and statistically obtain risk probabilities from the 'future selves'. The method can better capture the uncertainty of disease development, provide a probability distribution instead of a single point estimate, and the prediction result is more in line with the randomness nature of a biological process.

A system for detecting and quantifying platelet function and coagulation function within a biological sample is provided. The system includes a detection instrument with computing capability. The system includes an assay device capable of receiving a biological sample. Introduction of the biological sample into the assay device results in a biological process by which fibrin and platelets may accumulate at a reaction zone of the assay device. The assay device is capable of receiving one or more chemical reagents compatible with the biological sample and usable for detecting the accumulation of the fibrin and platelets within the reaction zone. The fibrin and platelets, and their associated signals, accumulated at the reaction zone of the assay device are usable to determine at least one of a platelet function, a coagulation function, platelet normalcy, fibrin normalcy, platelet response to a drug, and coagulation response to a drug, within the biological sample.

Methods and apparatus for generating values for parameters of a model defining a relationship between one or more biological processes of a biological system. A plurality of data sets are received, ea

A bioreactor system for carrying out a biological process including a vessel for receiving a liquid biological medium, and a gassing installation for controlled supply of different gases from gas sources into the vessel. The gassing installation has a plurality of gas outlet lines which open into gassing devices and / or into an overlay gas outlet in the interior of the vessel. Each gas outlet line is connected to a plurality of gas sources via a respective gas supply line or via a respective branch from a gas supply line. A mass flow controller connected to a controller is arranged in each gas supply line and in each branch. The system may include a controller with a master controller for a controlled variable, at least one sensor associated with the master controller, and one or more slave controllers having actuators, the manipulated variables of which purposefully influence the controlled variable.

The invention belongs to the technical field of biological process monitoring and optimization, and discloses a fermentation-hydrolysis process parameter automatic control system and method, and the method comprises the steps: collecting online process parameter time sequence data for pre-selected key process parameters; taking the online process parameter time sequence data as real-time input, and utilizing a pre-trained soft measurement model to obtain a key metabolite estimation concentration value sequence; calculating to obtain a key metabolite estimation generation rate value sequence based on the key metabolite estimation concentration value sequence; forming a real-time two-dimensional state vector based on the current estimated concentration value of the key metabolite and the current estimated generation rate value of the key metabolite; comparing and evaluating the real-time two-dimensional state vector with a predefined decision logic; when the decision logic judges that a preset triggering condition is met, a decision signal for starting hydrolysis is obtained; and the optimal opportunity for starting hydrolysis is accurately captured.

Methods of tagging cells with unique oligonucleotide “zipcode” constructs are provided. By these methods and associated compositions, cells in a multicellular structure such as a tissue section can be tagged with a construct, the unique composition of which is associated with the cells position in the multicellular structure. Subsequently, the multicellular structure can be dissociated into single cells and a single cell transcriptome analysis performed, as well as other types of single cell analyses. By preserving positional information in the analyzed single cells, biological processes within the tissue can be mapped. By these methods, the effects of the local environment surrounding a cell on its state and various functions can be elucidated, and intra-tissue processes can be mapped and observed. Likewise, coordinated actions by multiple cells within a tissue can be mapped and tracked over time.

The invention relates to the technical field of biological medicine, and particularly discloses application of nefenavir. Nefinavir is used for preparing a medicine for treating or preventing castration-resistant prostate cancer, and the medicine can specifically up-regulate expression of DHCR7, induce DNA damage of tumor cells and activate a cell cycle arrest pathway mediated by periodic protein of the cells so as to inhibit key biological processes such as proliferation of the tumor cells, cholesterol metabolism and the like; especially, formation and amplification of multinuclear giant cells in castration-resistant prostate cancer can be effectively inhibited by up-regulating expression of DHCR7, so that malignant progression of multinuclear giant cell mediated tumors and treatment of drug resistance are blocked. Finally, the castration-resistant prostate cancer is prevented and treated, and the technical bottlenecks that an existing castration-resistant prostate cancer treatment means is limited in curative effect, and the drug resistance and metastasis capability mediated by multinuclear giant cells are difficult to inhibit in a targeted manner are solved.

The invention discloses a method for preparing tobacco extract rich in terpenes by a biological method, which comprises the following steps: (1) grinding and sieving tobacco leaves in the middle of flue-cured tobacco, mixing with deionized water, extracting, filtering and sterilizing to obtain tobacco extract liquid; (2) denucleating bergamot, squeezing to obtain juice, adding tannase for constant-temperature reaction, and heating to inactivate the tannase after the reaction is ended, so as to obtain bergamot extracting solution; (3) respectively culturing micrococcus ZY-02 and klebsiella variicola HNYJ-3, and mixing the seed solutions according to the volume ratio of 1: 1 to obtain an aroma-producing strain seed solution; (4) mixing the tobacco extract with the bergamot extract according to the mass ratio of 100: 1-1: 1, and then mixing the mixture with the aroma-producing fungus seed solution according to the volume ratio of 1: 1000-1: 50 for fermentation; and (5) centrifuging the fermentation liquor, taking supernatant, concentrating, and adding 1, 2-propylene glycol to obtain the tobacco extract rich in terpenes. The terpenoid-rich tobacco extract is prepared by a biological method, so that the quality of the tobacco extract can be effectively improved.