771 results about "Microbiome" patented technology

The invention discloses a water quality monitoring system applied to sewage treatment, and relates to the technical field of sewage quality monitoring. Before microorganisms are added, the uniformity of water quality is monitored and analyzed, the sewage treatment mode is confirmed, and during layered treatment, the water quality is monitored and analyzed. The method comprises the following steps: analyzing a microorganism combination scheme and a microorganism addition scheme during sewage treatment of each deep layer, during overall treatment, carrying out water quality monitoring on the microorganism combination scheme and the overall microorganism addition scheme of the sewage after microorganisms are added, and then carrying out corresponding adjustment, so that the uniformity and uniformity of the water quality treatment progress are ensured; the method is beneficial to maintaining the stability of a microorganism growth environment, so that microorganisms continuously and efficiently work in a relatively stable environment, the decomposition speed of pollutants in sewage is increased, the stability of effluent quality is ensured, the difficulty of subsequent microorganism control and regulation is reduced, the overall sewage treatment effect is improved, microorganism waste is not easily caused, and the method is suitable for industrial production. The operation cost is reduced.

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

The invention provides an IBS micro-ecological transplantation intelligent prediction method and system based on multi-omics driving, and relates to the technical field of biomedicine. The method comprises the following steps: establishing a multi-omics data fusion subsystem to collect metagenome, metabolome, host genome and clinical phenotype group data of a target patient; inputting the data into a flora-metabolite combined network analysis model to construct an interaction network and extracting features; generating an incidence matrix based on the features and the host genome data and calculating indexes; generating indexes through a dynamic response algorithm in combination with the clinical phenotypic data and the indexes; and outputting a curative effect prediction result by using a transfer learning framework combined with modeling. The system comprises a data acquisition module, a network analysis module, a correlation calculation module, a dynamic response module and a joint modeling module. According to the method, multiple omics data are integrated, the flora and host relation is accurately mined, intelligent prediction of the micro-ecological transplantation curative effect is achieved, powerful support is provided for IBS personalized treatment, and meanwhile data processing and safety guarantee measures are taken.

The invention discloses a method and system for predicting juvenile depression based on intestinal flora, and relates to the technical field of bioinformatics and artificial intelligence, and the method comprises the steps: firstly, obtaining an original sequence of a microbiome, carrying out the preprocessing of the original sequence of the microbiome, and obtaining a feature matrix; and screening core flora characteristics with stable trans-folding by adopting characteristic importance evaluation and interpretability analysis based on a gradient boosting decision tree. A mixed weighted graph is constructed based on Spearman correlation and a proximity relationship, and an absolute value of a correlation coefficient is taken as an edge weight and an edge density is adjusted through a threshold adaptive strategy. And finally, through an improved graph attention neural network, based on edge weight attention, layer normalization and random inactivation, enhancing robustness, and adopting adaptive optimization to complete parameter learning. And determining a dynamic classification threshold according to the AUC of the target patient, and outputting a sample discrimination result and confidence. According to the method, the accuracy, stability and biological interpretability of juvenile depression recognition are remarkably improved.

The invention discloses a biocontrol complex microbial inoculant for preventing and treating maize curvularia leaf spot and application thereof, and belongs to the field of crop disease prevention and treatment. The biocontrol complex microbial inoculant comprises the following active ingredients: bacillus cereus, pseudomonas chlororaphis and streptomyces microflavus, and / or metabolites thereof. The beneficial microorganisms with different action mechanisms are combined, and the prevention and control effect on the curvularia maize leaf spot disease is enhanced through the synergistic effect. Natural microbial resources are adopted to replace traditional chemical pesticides, harmful substance residues are reduced, and the ecological environment is protected. Through reasonable matching of different types of beneficial microorganisms, effective prevention and control of the curvularia maize leaf spot disease are realized, and healthy growth of plants can be promoted. Compared with a single strain preparation, the complex microbial inoculant provided by the invention has stronger stability and durability, and can significantly reduce the incidence rate of diseases.

The invention provides a soil flora analysis method, a related soil flora and application thereof. Based on a soil long-term positioning test, an absolute quantitative method is used for the first time to explore a dynamic assembly rule of rhizosphere and in-root microbiome in a plant full life cycle, and the response characteristics of the rhizosphere microbiome to different fertilization treatments are studied. By combining methods of metagenome, microorganism isolated culture, community synthesis and the like, functional adaptation of rhizosphere microorganisms under different fertilization conditions and a mechanism of influencing the growth of leguminous plants are disclosed. The invention also provides a method for improving the chemical properties and soil flora of soil; the invention also provides an artificially synthesized flora which has a promoting effect on the growth and biomass of leguminous plants.

The present invention discloses a disease prediction method and system based on adaptive fusion of microbiome hierarchical features, which relates to the technical field of microbiome data analysis. A trained disease prediction model is used to predict diseases based on microbiome data. The disease prediction model performs multi-level feature extraction on feature data of five classification levels through a hierarchical feature extraction module, and integrates the results into multi-level fusion features. The multi-scale feature fusion module processes the multi-level fusion features through three parallel channels of pattern flow, context flow and content flow to achieve an all-round representation of microbiome data. The dynamic sampling module is introduced in the training process, and based on the class balance mechanism and the uncertainty evaluation strategy, the sample sampling weights are dynamically adjusted. Finally, a classifier is used to predict diseases for the multi-scale fusion features. The present invention makes full use of microbiome classification level information, realizes multi-dimensional feature representation, effectively addresses the problem of sample imbalance, and improves the accuracy of disease prediction.

The invention discloses a method for improving saline-alkali soil by using arbuscular mycorrhizal fungus bacterial fertilizer, and relates to the technical field of biology. The arbuscular mycorrhizal fungus bacterial fertilizer comprises a microorganism combination for improving saline-alkali soil, the microbial composition comprises Scopulariopsis brevicaulis (Scopulariopsis brevicaulis), Absidia corymbifera (Absidia corymbifera) and Eurotium chevalieri (Eurotium chevalieri), and is characterized in that the microbial composition comprises Scopulariopsis brevicaulis, Absidia corymbifera and Eurotium chevalieri (Eurotium chevalieri). The method for improving the saline-alkali soil by using the arbuscular mycorrhizal fungus bacterial fertilizer provided by the invention is a green, environment-friendly and efficient soil improvement technology, can effectively improve the physicochemical properties of the saline-alkali soil and improve the soil quality and crop yield, and has wide market prospects and important social significance.

The invention belongs to the technical field of microbial agents, and particularly relates to a preservative and fresh-keeping microbial composition as well as a microbial agent and application thereof. The microbial composition disclosed by the invention comprises bacillus subtilis LS and bacillus velezensis LX, and in the microbial composition, the concentration ratio of the bacillus subtilis LS to the bacillus velezensis LX is (0.1-1): (0.1-1); the preservation number of the bacillus subtilis LS is CGMCC (China General Microbiological Culture Collection Center) NO.30953; the preservation number of the bacillus velezensis LX is CGMCC (China General Microbiological Culture Collection Center) NO. 30954. In the microbial composition provided by the invention, the bacillus subtilis LS and the bacillus velezensis LX can generate a synergistic effect and can be used for preventing and treating the soft rot of the kiwi fruits, and a theoretical basis and technical guidance are provided for biological prevention and treatment and storage and fresh-keeping of the soft rot of the kiwi fruits.

The invention discloses methanogen lyase homologous with a PeiR lyase sequence and application of the methanogen lyase, and aims to solve the problem that PeiR lyase protein of a methanogen cell wall peptide bond can participate in a biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the PeiR lyase protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogens virus from rumen microbiome sequencing data, and sequence homology between the proteins and PeiR protein is further analyzed to obtain a series of methanogens lyases. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.

The invention discloses a soybean phospholipid disease-preventing and root-promoting microbial agent as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises a microbial composition, modified enzymolysis soybean phospholipid, a carrier and auxiliary components. According to the invention, the modified enzymolysis soybean phospholipid and microorganisms are combined to achieve a synergistic effect. According to the method, soybean phospholipid is modified and subjected to enzymolysis, and the steps of pretreatment, stepped low-temperature treatment, enzymolysis treatment, enzyme inactivation and purification and the like are carried out, so that the quality and performance of the soybean phospholipid are improved, the rhizosphere colonization rate of strains can be increased, the disease prevention and root promotion effects can be improved, the soil micro-ecological environment can be improved, and good growth conditions are created for plant root systems; and the disease-preventing and root-promoting effects of the microbial agent are further enhanced. The microbial agent disclosed by the invention can be used for remarkably reducing the morbidity of wheat sharp eyespot and reducing the use amount of chemical pesticides, can also be applied to planting of other crops, and can play a role in preventing and controlling various plant diseases and promoting root growth.

The invention belongs to the field of polypeptides, and particularly relates to an antibacterial peptide derived from marine microorganisms, an antibacterial composition and application. Based on a deep learning algorithm, five potential antibacterial peptides are mined from a global marine microbiome database, and the five antibacterial peptides are expressed in yarrowia lipolytica. Experimental tests show that the five antibacterial peptides have differentiated antibacterial effects on different bacteria, and the antibacterial peptide shown in SEQ ID NO: 3 can have an obvious inhibition effect on escherichia coli, pseudomonas aeruginosa and staphylococcus aureus, has low hemolysis and has a positive application prospect. The antibacterial peptide disclosed by the invention has the potential of further developing novel antibacterial drugs or antibacterial additives.

The present invention relates to a microbial combination and a fermented milk and the use thereof, in particular to a multi-strain microbial combination, a fermented milk prepared from the microbial combination and the use of the microbial combination in the fermentation of a camel milk. The microbial composition contains at least two of lactic acid bacteria and yeasts derived from traditional fermented milk products in Xinjiang, including Leuconostoc pseudomesenteroides, Lactobacillus rhamnosus, Debaryomyces hansenii and Kluyveromyces marxianus. The microbial composition ensures that industrially fermented camel milk products have the flavor and nutritional functions of naturally fermented sour camel milk, but with higher quality, better safety and significantly prolonged shelf life. The microbial composition has relatively high acidification efficiency, proteolysis capacity and lactose decomposition capability, can improve the unpleasant flavor of camel milk products industrially fermented by using conventional strains, and has wide application prospects in industrial production.

The invention relates to the technical field of microbial agents, and discloses a plant root system growth-promoting microbial agent screening method which comprises the following steps: firstly, collecting rhizosphere microbiome data and corresponding crop growth phenotype data of different target crops in growth stages and soil environments; then establishing a microorganism-phenotype prediction model based on the data, and obtaining two groups of key growth promoting strains through feature importance analysis and microorganism interaction network analysis; finally, according to the key strains and the prediction model, screening out a microbial inoculum formula with the optimal expected growth promoting effect. According to the invention, a set of systematic screening scheme for the plant root growth-promoting microbial agent is established, by comprehensively considering the growth-promoting effect and the interaction relationship of the strains, an efficient and synergistic dominant strain combination can be screened out, the pertinence and the effectiveness of the plant root growth-promoting microbial agent are remarkably improved, and the method has a good application prospect in the field of microbial fertilizer development.

The present invention discloses a water quality monitoring system applied to sewage treatment, which relates to the technical field of sewage water quality monitoring. Before adding microorganisms, the present invention monitors and analyzes the uniformity of water quality, confirms the sewage treatment mode. During stratified treatment, it analyzes the microorganism combination plan and microorganism addition plan for sewage treatment at each depth layer. During overall treatment, it analyzes the microorganism combination plan and overall microorganism addition plan for sewage, and after adding microorganisms, it conducts water quality monitoring and then makes corresponding adjustments to ensure the unity and uniformity of the water quality treatment progress, which helps to maintain the stability of the microorganism growth environment, so that the microorganisms can continuously work efficiently in a relatively stable environment, improve the decomposition speed of pollutants in the sewage, ensure the stability of the effluent water quality, reduce the difficulty of subsequent microorganism control and regulation, improve the overall sewage treatment effect, and is not easy to cause microorganism waste, reducing the operation cost.

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiR lyase and application of the methanogen lyase and aims to solve the problems that PeiR lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, methanogen is effectively killed, methane production is reduced, and protein homologous with the protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogen viruses from a large amount of rumen microbiome sequencing data, and high-dimensional homology between the proteins and PeiR proteins based on a deep learning model TM-Vec is further analyzed, so that a series of methanogen lyases are obtained. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.

The invention relates to the technical field of microorganisms, in particular to a complex microbial inoculant for resisting salmonella infection and application of the complex microbial inoculant. The invention provides a microbial composition for resisting salmonella infection. The microbial composition comprises lactobacillus reuteri, lactobacillus mucosa, lactobacillus plantarum, lactobacillus bulgaricus and pediococcus pentosaceus. Results of the embodiment of the invention show that the complex microbial inoculant provided by the invention has an excellent anti-salmonella typhimurium effect, and only the anti-salmonella typhimurium effect of the complex microbial inoculant in a specific proportion provided by the invention is optimal; meanwhile, the complex microbial inoculant can effectively relieve the harm of salmonella typhimurium to chickens, improve the growth performance of the chickens and improve the immunity of the chickens. The invention provides a new prevention and treatment thought for salmonella.

The invention discloses a novel method for food allergy intestinal flora target mining, and belongs to the technical field of target mining. The new method comprises the following steps: acquiring whole genome associated GWAS data and intestinal flora related data of food allergy; performing tool variable screening and multi-effect removal on the collected data; according to the collected tool variables, analyzing the data by adopting a bidirectional Mendel randomization method to obtain a preliminary screening result of the causal flora; the preliminary screening result is analyzed, and candidate tool variables are obtained by integrating effect values of multiple analysis methods and judgment of sensitivity analysis; establishing a food allergy animal model; carrying out targeted determination on the screened related flora having a causal relationship with food allergy by adopting a microbiological analysis method; and the active small molecules act on the target flora to determine the reliability of the active small molecules as the food allergy target. According to the method, the intestinal flora and the target flora of food allergy can be determined.

The invention discloses a feed additive for improving mutton quality and producing low-mutton-smell mutton, which is characterized in that through combined application of phytosterol and dl-alpha-tocopheryl acetate, the microbial composition of the lamb intestinal tract is improved, especially the abundance of beneficial bacteria is improved, and the abundance of potential pathogenic bacteria is reduced. The combined use of the two can improve the intestinal microbial ecology, and significantly reduce the drip loss, shear force and Plt of mutton; 0.05, the mutton color and the tenderness of the mutton are improved; excrement causing secondary pollution to the environment is not generated. The application of the plant extract in the production of the low-mutton-smell mutton is reported for the first time, the mutton smell can be remarkably reduced by combining and adding the phytosterol and the dl-alpha-tocopheryl acetate according to a certain proportion, and meanwhile the mutton quality is improved. The feed additive disclosed by the invention is simple in components, remarkable in effect and simple and convenient to apply, and has great potential popularization and application values.

The invention provides a directional enrichment method of soil active organic carbon, a biochar-microorganism composite preparation and a preparation method, and relates to the technical field of soil improvement and agricultural sustainable development. The method comprises the following steps: (1) screening biochar; (2) screening and culturing a functional microbiome which at least comprises carbon-decomposing bacteria, nitrogen-fixing bacteria and phosphate-solubilizing bacteria, and compounding the carbon-decomposing bacteria, the nitrogen-fixing bacteria and the phosphate-solubilizing bacteria according to a CFU ratio of (1-5): (1-3): (1-2); (3) compounding the biochar in the step (1) and the functional microbiome in the step (2) by adopting an adsorption-embedding combined process to form a biochar-microbiome synergistic regulation system; and (4) applying the charcoal-microbiome coordinated regulation system obtained in the step (3) to target soil, and culturing. According to the invention, not only is input of soil organic carbon increased, but also accurate and efficient enrichment of the soil active organic carbon library is realized.

The invention belongs to the field of microbial agents, and particularly relates to a microbial composite agent for degrading herbicides and a preparation method of the microbial composite agent. The preparation method of the microbial complex microbial inoculant comprises the following preparation steps: mixing bran hydrolysate, peat soil and diatomite, sterilizing, adding a protective agent and a complex flora suspension in a sterile environment, uniformly mixing, kneading and forming, preparing into slices, naturally air-drying, and crushing to obtain the microbial inoculant, and uniformly mixing the microbial agent with the adsorbent, the surfactant and the nutrient substances to obtain the microbial complex microbial inoculant. According to the invention, domesticated microorganisms form a composite flora to prepare the microbial agent, and the composite flora exerts unique degradation capability and generates synergistic effect, so that the herbicide can be degraded more comprehensively and efficiently, and the proportion of each microorganism, an adsorbent, a surfactant and other components in the microbial agent can be adjusted according to different herbicide types and polluted environments; and efficient degradation of specific herbicides is realized.

The invention relates to the technical field of microbial soil remediation, in particular to a microbial composition for remediation of saline alkali soil, a microbial agent and application of the microbial agent. According to the characteristics of the bacillus subtilis KC000 and the paenibacillus mucilaginosus JDY001, different compounding proportions are set and applied to the saline-alkali soil, then the salt content of the saline-alkali soil is measured, and it is found that the soil nutrition and environmental conditions are obviously improved by applying the microbial agent, and the saline-alkali soil can be applied to the saline-alkali soil. The soil conditioner is beneficial to the growth and reproduction of paenibacillus mucilaginosus and bacillus subtilis in soil, is beneficial to improving the content of organic matters, rapidly available phosphorus and rapidly available potassium in the soil, increases the content of alkali-hydrolyzable nitrogen in the soil to a certain extent, reduces the pH value of the soil, accelerates salt leaching, inhibits salt return and reduces the salt content of the surface layer of the soil. Meanwhile, the microbial composition also has the effect of preventing and treating sheath blight.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

Provided is a Bifidobacterium lactis. The Bifidobacterium lactis is Bifidobacterium animalis subsp. lactis BL-11, and the preservation number of the Bifidobacterium animalis subsp. lactis BL-11 is CGMCC No. 20847. The provided Bifidobacterium animalis subsp. Lactis can improve intestine metabolism disorder and normalize the composition of intestine microbiome so as to improve height, suppresses obesity, promote infant and youth physical development and mental development, and prevent and treat mental disorders, said mental disorders including anxiety, depression, attention-deficit / hyperactivity disorder, autism, schizophrenia, hepatic encephalopathy, anorexia, Tourette syndrome, and Asperger syndrome.

The present invention relates to compositions for use in treating cardiovascular disease (CVD) and / or gut dysbiosis, and methods of treating CVD and / or gut dysbiosis. In particular, the present invention relates to compositions for use and methods for treating cardiovascular disease (CVD) and / or gut dysbiosis in subject groups having a reduced gut gene count (faecal microbiome gene count).

The present invention generally provides methods and compositions for the treatment of Parkinson's disease and depression and / or anxiety. The invention relates to recombinant microorganisms, particularly gut-colonizing probiotics, modified to produce L-DOPA.

The disclosure relates to compositions, including ready-to-use spray or wipe compositions, that are non-toxic, non-corrosive, biodegradable acidic antimicrobial compositions that exhibit broad-spectrum antimicrobial efficacy, wherein the compositions comprise an amine oxide, such as an alkyl amine oxide, in combination with an acidulant. Beneficially, the acidic antimicrobial compositions are capable of achieving at least a 3-log microbial reduction within 5 minutes under relatively mild pH conditions. The compositions provide cleaning and sanitizing.

The present invention includes modified and unmodified microbes, compositions comprising these microbes, and methods of using these microbes and / or compositions for enhancing plant health, plant growth plant yield and / or drought tolerance. This disclosure also provides non-naturally occurring plant varieties that are artificially infected with microbes descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.

The invention relates to methods and compositions for preventing microbial infections. Methods and compositions for permanently affecting the microbial ecosystem (microbiome) in a subject to prevent infection and reduce recurrence of microbial infections are provided. In some embodiments, compositions and methods are provided for the production and use of molecularly modified bacterial strains having potential to prevent infection with a variety of microorganisms.