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640 results about "False positive rate" patented technology

In statistics, when performing multiple comparisons, a false positive ratio (or false alarm ratio) is the probability of falsely rejecting the null hypothesis for a particular test. The false positive rate is calculated as the ratio between the number of negative events wrongly categorized as positive (false positives) and the total number of actual negative events (regardless of classification).

Leakage detecting and locating method based on pressure and sound wave information amalgamation

A leakage detection location method based on pressure and sound information integration is provided, which belongs to the technology field of an oil (gas) pipeline fault diagnosis, and is characterized by leakage detection and leakage location based on information integration. The former comprises: separately acquiring the measurement data of the pressure on the upperstream end and downstream end of the pipeline and a sound wave sensor, sending the data to a computer and gaining final detection results through three levels processing including data filtering, characteristic level integration and decision level integration. If the detection result shows leakage, starting leakage location process based on information integration. The process first carries out leakage location by separately adopting the signals of two kinds of sensors and a plurality of different leakage location algorithms and finally gains the location results via integration of the location results based on the same sensor and different location methods and two levels processing based on the integration of the location results of two kinds of different sensors. The method can effectively reduce the false positive rate and false negative rate and improve the location precision.
Owner:TSINGHUA UNIV

Copy number variation detection method based on next generation sequencing

The invention discloses a copy number variation detection method based on next generation sequencing. The method comprises the following steps: pre-processing copy number variation data, constructing a sliding window, calculating of statistics, implementing a replacement policy, constructing zero distribution, and carrying out performance evaluation of an algorithm. The performance evaluation of the algorithm comprises the steps of judging whether a relatively high correct positive rate can be acquired by the algorithm under the condition that a false positive rate is controllable, evaluating whether the algorithm can relatively accurately estimate a p value or not, detecting a boundary detection capability of copy number variation, and analyzing the calculation complexity of the algorithm. With the adoption of the copy number variation detection method, the problem of copy number variation detection errors, caused by the fact that sequencing platforms and sequencing levels are different, is solved, and a result is relatively accurate; data is normalized by utilizing characteristics of a multi-peak frequency histogram, so that a normal region and a copy number variation region are accurately divided; and a new model is established by a comprehensive effect of relevance between a variation reads number and a variation site, so that the inconsistency problem is solved, and the remarkable level of copy number variation is objectively estimated.
Owner:XIDIAN UNIV

Method for rapidly obtaining CRISPR/Cas9 (clustered regularly interspersed short palindromic repeats/Cas9) gene knockout stable cell line through monoclonal cell sorting

InactiveCN107418974AImprove knockout productivitySave the trouble of screeningVectorsStable introduction of DNAFluorescenceHigh survival rate
The invention relates to a method for rapidly obtaining a CRISPR/Cas9 (clustered regularly interspersed short palindromic repeats/Cas9) gene knockout stable cell line through monoclonal cell sorting and belongs to the technical field of genetic engineering and genetic modification. According to the method, a CRISPR/Cas9 system, single-cell sorting by a flow cytometer and fluorescent protein screening on an expression vector are combined, positive monoclonal cells can be obtained in short time, and gene knockout work efficiency of the cell line is greatly improved. Compared with a traditional cell line gene knockout method, the flow cytometer is utilized for single-cell sorting, on one hand, tedious work of antibiotic screening is omitted, so that a large quantity of single cells can be obtained in quite short time, and on the other hand, the condition that cells in each culture hole are single cells can be guaranteed and false positive rate is reduced. Sorting is performed 40-80 h after cell transfection, at the time point, the highest survival rate of the cells after sorting can be guaranteed, and the screening efficiency is improved accordingly.
Owner:XINXIANG MEDICAL UNIV
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