114 results about "Cytology" patented technology

The invention belongs to the technical field of medical image processing, and provides a bone marrow cell WSI region-of-interest automatic analysis positioning method and system, and the method comprises the steps: obtaining a full glass slide bone marrow aspiration smear image (WSI); a pyramid network is adopted to zoom the full-glass-slide bone marrow aspiration smear image, and a low-resolution image is generated; designing a general area condition prompt strategy by combining bone marrow cytology medical priori knowledge, and calling a language model to construct text description of a bone marrow microparticle area for the low-resolution image; using a visual language model to align the text and the low-resolution image, and then adopting a feature visualization technology and based on gradient weighted channel selection to generate a semantic response graph; the dispersion degree of a semantic response region in the semantic response graph is calculated, and segmentation prompt information is generated by adopting a region perception and strategy self-adaption mechanism; and inputting segmentation prompt information and the low-resolution image into an SAM model, generating an ROI mask image through reasoning, and completing positioning of the bone marrow cell WSI region of interest.

A method for diagnosing nasal cytology based on deep learning includes: reading in blocks a nasal cytology to be diagnosed in a sliding window; performing preprocessing on the window image and inputting the preprocessed window image into a trained cell detection model, a coordinate and a score of a bounding box of a detected target being obtained based on feature maps, and a bounding box set being obtained by filtering out bounding boxes with scores below a predetermined threshold; cropping an image from the window image based on coordinates of bounding boxes in the bounding box set, performing preprocessing on the image and inputting the preprocessed image into a trained cell classification model to output a cell category of the corresponding image, and performing post-processing to obtain a cell category of the nasal cytology to be diagnosed.

The invention relates to the technical field of biological detection, and discloses a reagent composition and a kit for detecting cervical cancer and precancerous lesions of cervical cancer and application of the reagent composition and the kit. The reagent composition provided by the invention is used for detecting the methylation degree of a specific marker gene, and the detection sensitivity and specificity of cervical cancer and high-grade cervical cancer precancerous lesions are at a relatively high level. When the reagent composition is used for screening cervical cancer and precancerous lesions of HPV positive people, compared with conventional cytological detection, the colposcope referral rate is greatly reduced, meanwhile, the detection times for definite diagnosis of high-grade cervical lesions or cervical cancer are reduced, and the waste of medical resources and the examination burden of patients are reduced.

Provided is a microscopic layered image data analysis device for analyzing microscopic z-stack images for use in cytologic diagnosis. This microscopic layered image data analysis device for a specimen S containing a large number of cells comprises: a microscope (20) that acquires z-stack images of the specimen S; an imaging unit (30) that images the z-stack images acquired by the microscope (20); a storage unit (70) that stores the z-stack images captured by the imaging unit (30); and a control unit (50) that detects cells from the z-stack images stored in the storage unit (70), acquires a cell image group corresponding to each of the detected cells, crops a cell image from images forming the cell image group for each of the cells, acquires the confidence level of each of a plurality of class classifications for each of the cell images of the cells, and visualizes the confidence level acquired for each of the class classifications.

The invention relates to the technical field of biological detection, and discloses a reagent composition for detecting urothelial carcinoma, a kit and application. The composition provided by the invention is used for detecting the methylation state of a specific target sequence in a characteristic gene of the urinary tract epithelial cancer, and the detection sensitivity and specificity of the urinary tract epithelial cancer are at a relatively high level. The composition provided by the invention can be used for noninvasive urinary tract epithelium cancer screening, and when the composition is used for urinary tract epithelium cancer screening, compared with conventional urine cytology examination and iconography examination, the detection sensitivity is greatly improved, the early screening accuracy of urinary tract epithelium cancer is improved, and the misdiagnosis rate and the missed diagnosis rate are greatly reduced.

The invention discloses application of licochalcone as a drug sensitizer and an anti-tumor drug composition. The application comprises an application of the acanthochalcone in preparation of an anti-tumor drug sensitizer and an application of the acanthochalcone in improvement of the anti-tumor efficacy of platinum chemotherapeutic drugs, and the invention further provides an anti-tumor composition capable of efficiently inhibiting platinum drug-resistant gastric cancer cells based on the application. Through a gastric cancer organ model and an in-vitro cytology experiment, it is found that the acanthochalcone has an inhibiting effect on proliferation of gastric cancer cells, and the sensitivity of the gastric cancer cells to platinum drugs can be remarkably enhanced. The invention also finds that IC50 of gastric cancer cells to cis-platinum and oxaliplatin, especially platinum acquired drug-resistant gastric cancer cells, can be significantly reduced by combined use of the acanthochalcone and platinum drugs. Therefore, the invention provides a new medication basis and choice for overcoming acquired platinum drug resistance of gastric cancer by utilizing the acanthochalcone.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The invention belongs to the technical field of plant cytology, and particularly relates to a method for improving the transformation efficiency of plant protoplast, which comprises the following steps: preparing enzymatic hydrolysate: each liter of enzymatic hydrolysate contains 20 mmol of KCl, 5-20 g of cellulase, 2-6 g of macerozyme, 10 mmol of CaCl2, 1 g of BSA and the balance of water; performing leaf enzymolysis; obtaining a protoplast; preparing a competent protoplast; resuspending the MMG solution; and transforming and incubating the protoplast resuspension by using the target plasmid. According to the method, the high-quality protoplast can be obtained, and when the protoplast is applied to downstream experiments, the conversion efficiency and stability of the experiments can be remarkably improved.

The present invention relates to a computer-implemented method for producing a trained model for detecting one or more defined cell types and / or cellular indicators, such as biomarkers or cellular abnormalities, in particular genomic aberrations, from single-cell images obtained from a sample of a body liquid, bone marrow, cytology smear or slide preparations, in particular a blood smear sample. It relates also to a computer-implemented method for detecting one or more defined cell types and / or cellular indicators, such as biomarkers or cellular abnormalities, in particular genomic aberrations, from single-cell images obtained from a sample of a body liquid, bone marrow or cytology smear, in particular a blood smear sample.

The invention relates to a compound as shown in a formula (I) and / or a formula (II) and / or a formula (III) or a tautomer or pharmaceutically acceptable salt thereof, a preparation method and application of the compound or the tautomer or the pharmaceutically acceptable salt in medicine. Specifically, the fluorescent probe can be used as a fluorescent probe for cytological imaging, can also be used as a near-infrared fluorescent imaging probe for diagnosing benign / malignant lesions such as fibrosis, nodules and tumors, and can also be applied to research of related biological events in cytobiology and molecular biology. The fluorescent probe can be particularly applied to fluorescent navigation in tumor resection surgery.

The invention discloses a method and device for identifying helicobacter pylori based on artificial intelligence fluorescence imaging, and relates to the technical field of artificial intelligence medical detection. Comprising the following steps: S1, carrying out slide preparation treatment on a cytological sample or a tissue slice sample, and processing the slide preparation through an immunofluorescent staining reagent to obtain a helicobacter pylori fluorescent staining picture; s2, outputting and obtaining a helicobacter pylori picture with a rectangular frame identifier through a helicobacter pylori identifier neural network model; and S3, according to the coordinate information of the rectangular frame, obtaining the coordinate information of the helicobacter pylori in the fluorescent staining picture, and splicing the helicobacter pylori pictures to obtain a spliced picture. According to the method, the helicobacter pylori is marked automatically through the helicobacter pylori identification neural network model, the problems of subjectivity and time consumption of traditional manual microscopic examination are avoided, meanwhile, the positions of thalli are accurately marked, positive probability data are output, and then the detection efficiency is improved.

Provided are a centromere protein CENP-M small molecule inhibitor cenpemlin, and a preparation method therefor and the use thereof. A small molecule compound cenpemlin is obtained by means of analyzing the protein plane information of the fine structures of CENP-M and CENP-L and performing targeted screening. The results of biochemical and cytological experiments all indicate that cenpemlin inhibits the proliferation of cancer cells by means of interfering with the interaction between CENP-M and CENP-L, thereby providing a new compound for analyzing and interfering with the rapid proliferation of cancer cells.

The invention relates to the technical field of liquid-based cytology, and particularly discloses serosa effusion cell preserving fluid. Comprising a preservation solution, an anticoagulant, a red blood cell treatment solution, a cell membrane stabilizer, an antioxidant and a metal ion chelating agent, the preservation solution contains a buffer system, an energy supply component, an antibiotic and a surfactant, and the red blood cell treatment solution contains a hemolysis active component and a cell immobilization component; the preparation method comprises the following steps: firstly, preparing the preserving fluid, adjusting the pH value, adding the active substances, mixing the antibiotics, and then completing constant volume and sterile filtration; the anticoagulant solution and the red blood cell treating fluid are independently prepared, and sterilization is achieved through filtration; and finally, packaging all the components in a sterilized storage bottle in a matched manner. The composition provided by the invention can be used for preserving cells in a clinical serosal effusion sample, and can provide a high-quality sample for subsequent cell morphology observation or detection; in addition, according to the preparation method, the controllability is high when all the components are independently prepared, and the preserving fluid with the consistent performance can be stably prepared.

The invention discloses an application of a novel phosphodiesterase 4 (PDE4) inhibitor ZL-n-91 in preparation of drugs for treating growth and metastasis of small cell lung cancer. In-vitro cytology experiments and in-vivo zoology experiments show that the phosphodiesterase 4 inhibitor ZL-n-91 can significantly inhibit proliferation and metastasis of small cell lung cancer, and the phosphodiesterase 4 inhibitor ZL-n-91 is expected to treat small cell lung cancer and has good development and application prospects.

The application discloses a marker group for cervical cancer screening and diagnosis and application thereof, and belongs to the field of molecular biological technology.The marker group comprises at least four polypeptides shown in sequences of SEQ ID NO.1-42.The marker group constructed by the application has more excellent sensitivity and specificity when used for auxiliary diagnosis and early screening of cervical cancer.The auxiliary diagnosis result is superior to the efficiency (sensitivity 81.1%, specificity 78.9%) of combined detection of serum CEA, SCC-Ag and CA125 in early diagnosis of cervical cancer;the diagnostic sensitivity and specificity of serum cf-DNA are 72.2% and 81.7% respectively.The early screening result is also superior to the internationally recognized cervical cancer screening method, i.e., combined screening of cytological detection (TCT) and human papilloma virus (HPV) virology detection.

The application relates to the field of cytology and relates to an oligonucleotide probe set design for identifying Brassica rapa chromosomes and application, which comprises the following steps: S1: obtaining a NR-TR set in a Brassica rapa R-o-18 reference genome; S2: statistically clustering the NR-TR set and obtaining TR array data of Brassica rapa chromosomes; S3: obtaining a repetitive sequence cluster which is enriched in all the chromosomes of Brassica rapa; S4: segmenting a repetitive sequence representative array of the obtained repetitive sequence cluster; and S5: respectively aligning the segmented sequences to the Brassica rapa R-o-18 reference genome by using blastn. The application establishes a technology for coating Brassica rapa chromosomes, and the technology can batch coat Brassica rapa chromosome materials.

A computer-implemented method for cytological grading includes receiving (302, 402) first image data (102) representing a biological sample, and parsing (304, 404) the received first image data into a plurality of patches (104). Each of the plurality of patches represents a corresponding portion of the received first image data. The method further includes identifying (306, 406) at least one cytological feature of each of the plurality of patches (108) using a first trained machine learning model (106), determining (308, 408) at least one statistic (110) based on the identified at least one cytological feature of each of the plurality of patches using a second trained machine learning model, and outputting (310, 410) the determined at least one statistic.

The present invention relates to a method for providing information for predicting the likelihood of bladder cancer recurrence, which provides information that patients who have undergone transurethral resection of a bladder tumor and test positive for suspicious high-grade urothelial carcinoma (SHGUC) or high-grade urothelial carcinoma (HGUC) in a urine cytology test have a higher likelihood of bladder cancer recurrence compared with patients who do not test positive.

The application discloses a biological additive for promoting poultry feather development and application thereof, and belongs to the technical field of animal breeding. The biological additive is prepared from scallop skirt oligopeptide and cantharides polypeptide at a mass ratio of 1:1. Animal experiments show that, when meat chickens are fed with 60 mg of the biological additive per 1 kg of basic feed, the growth of the meat chickens can be promoted, and more importantly, the feather development of the meat chickens can be significantly and synergistically promoted, and the length of the main wing feathers is obviously increased. Cytological studies further prove that the biological additive can synergistically promote the proliferation of poultry hair follicle epidermal stem cells, and the effect is better than that of an EGF positive control. Therefore, the biological additive developed by the application is safe, efficient and green, effectively solves the problems of drug residues and drug resistance possibly caused by traditional chemical additives, provides a sustainable solution for poultry breeding, and has important economic and application values.

The invention provides a computer-implemented method for assisting diagnosis of dysplasia from a liquid-based cytology whole-slide image (WSI), using a multi-stage pipeline combining deep- learning-based detection with engineered feature extraction and a calibrated machine-learning classifier. The method involves receiving and resampling a digital WSI to a canonical spatial resolution, detecting individual cell instances with a neural network, computing morphological and texture features for each detected cell, aggregating these features across the WSI to form a slide-level feature vector, inputting this vector into a calibrated classifier to yield a slide-level probability of dysplasia, computing slide-quality metrics to govern downstream triage, presenting results via an interactive graphical interface with per-cell thumbnails, and iteratively refining the system with expanded and harmonized training data from expert annotations.

The invention discloses a marker group for screening and diagnosing bladder cancer and application of the marker group, and belongs to the technical field of molecular biology. The marker group comprises at least four of polypeptides as shown in sequences SEQ ID NO. 1 to 35. The marker combination constructed by the invention has more excellent sensitivity and specificity when being used for auxiliary diagnosis and early screening of bladder cancer. The auxiliary diagnosis results of the selected marker combination are partially or completely superior to the urine cytology examination (the sensitivity is 62.5% and the specificity is 87.5%) recommended by the current bladder cancer clinical guide and the biomarker NMP22 (the sensitivity is 85.4% and the specificity is 76.5%) approved by FDA. The early screening results are partially or completely superior to the mass spectrum metabonomics OPLS-DA model (the sensitivity is 91.2%, and the specificity is 86.8%) recommended by the current bladder cancer early screening expert consensus.

The invention discloses an application of a novel phosphodiesterase 4 (PDE4) inhibitor ZL-n-91 in preparation of medicines for treating growth and metastasis of oral cancer. In-vitro cytology experiments and in-vivo zoology experiments show that the phosphodiesterase 4 inhibitor ZL-n-91 can significantly inhibit proliferation and migration of the oral cancer, reveals that the phosphodiesterase 4 inhibitor ZL-n-91 is expected to treat the oral cancer, and has good development and application prospects.

The application relates to the technical field of medical image database, and discloses a standardized cervical cancer pathological image database construction method, which comprises the following steps: step 1, specimen number is hashed to obtain pseudo-anonymous specimen number, unique slice identification and cell-level unique identification are generated; step 2, a unified reference system is established, the reference layer pixel spacing, the down-sampling multiple and the coordinate mapping are determined; step 3, the image is converted into an optical density space and normalized according to a target staining base to obtain a standardized image; step 4, cross-modal anchor point lists are established by manually comparing cytological and histological images; step 5, a hierarchical label set is generated by synthesizing multi-level unique labels according to the severity full sequence; step 6, the intersection-over-union index and the kappa consistency value are calculated according to the combination key, and a review to-do list is generated; step 7, point review and rule revision are carried out, and a data dictionary, a consistency report and an anchor point snapshot are output. The application realizes the standardized database construction and traceable quality control of cervical cancer cytological and histological pathological images.

A device (1) for detaching cells or tissue from a cavity in a subject, the device (1) comprising: an elongated member (200) arranged to be moved within a lumen of a needle (11) or a catheter; and at least one flexible member (300) arranged at a distal end of the elongated member (200), wherein the flexible member (300) is configured to be brought between a first, constrained configuration within the lumen of the needle (11) or catheter, and a second, expanded configuration outside said lumen, wherein the flexible member (300) in the second, expanded configuration is configured to conform to an inner geometry of a cavity in which the device (1) is inserted.

The invention discloses a cytology specimen pretreatment system and method based on machine vision and intelligent decision, and relates to the technical field of biological sample treatment.The cytology specimen pretreatment system comprises an operation platform, and the operation platform is provided with a first oscillation module, a centrifugal module, a visual recognition module and a sample tube operation assembly; automatic uniform mixing and centrifugal operation of sample liquid are achieved, so that early-stage materials meeting the requirements of cytology specimens are obtained, excessive manual intervention treatment is not needed, the labor cost and the infection probability in the operation process of medical staff are reduced, the working efficiency of early-stage treatment of the cytology specimens is improved, the requirement for experience of operators is low, and the operation cost is reduced. In addition, the cytological specimen is completely prepared, the cytological wax block and the thin layer are prepared, and the supernate is reserved, so that the opportunity of grafting the cytological specimen to other detection platforms subsequently is increased, and the value of the cytological specimen in pathological diagnosis is improved.

This invention belongs to the field of plant breeding and genetic engineering technology, specifically involving AtGalOxs Genes and their application in regulating plant organ size and development. Specifically, this invention screened a gene that regulates plant organ size and development. AtGalOxs It was identified as a key gene regulating the size and development of organs in Arabidopsis thaliana plants, and this was confirmed through cytological observation and transcriptome analysis. AtGalOxs Genes function by regulating cell proliferation and auxin signaling pathways, providing new theoretical basis for elucidating the molecular network regulating plant organ size; AtGalOxs The gene can be directly applied to the regulation of Arabidopsis plant architecture, and its homologous genes are conserved in crops such as Brassicaceae. This provides key gene resources for improving crop plant architecture and increasing yield, which is of great significance for promoting the development of plant molecular breeding technology and improving agricultural production efficiency.

The present technology relates to methods for determining whether a patient having thyroid nodules with indeterminate cytology will benefit from diagnostic surgery, e.g., lobectomy. These methods are based on screening a patient's thyroid nodules and detecting alterations in target nucleic acid sequences corresponding to a specific set of thyroid cancer-related genes. Kits for use in practicing the methods are also provided.

The utility model provides a pen-holding type electric rotating solid tumor cytology needle biopsy device. A puncture device comprises a shell, a rotating / front-back telescopic module, a negative pressure module and a puncture needle, wherein the rotating / front-back telescopic module and the negative pressure module are installed in the shell, and the puncture needle is installed at the end of the shell. The rotating / front-back telescopic module comprises a first driving motor, a driving gear, a driven gear, a first end cam and a second end cam, the first end cam and the second end cam can relatively rotate and axially reset, the first driving motor drives the driven gear to rotate, the driving gear is in meshing transmission with the driven gear, and the driven gear is coaxially and slidably connected with the first end cam. The tail portion of the first end face cam is detachably connected with the puncture needle. According to the puncture device, the pulling and inserting actions and the puncture distance in the puncture process can be accurately controlled, automatic or controllable negative pressure is achieved, the experience dependence of the puncture technology of an operator is remarkably reduced, the learning curve of the operator is effectively shortened, the effective rate of sample taking is improved, and the probability of complications possibly generated in the puncture process can be remarkably reduced.

The invention belongs to the technical field of plant breeding and genetic engineering, and particularly relates to an AtGalOxs gene and application thereof in regulation and control of size development of plant organs. Specifically, a gene AtGalOxs for regulating and controlling the size and development of the plant organs is screened and is clear as a key gene for regulating and controlling the size and development of the plant organs of arabidopsis thaliana, and cytological observation and transcriptome analysis prove that the gene AtGalOxs plays a role by regulating and controlling cell proliferation and auxin signal pathways, so that the gene AtGalOxs can be used for regulating and controlling the size and development of the plant organs of the arabidopsis thaliana. A new theoretical basis is provided for analyzing a molecular network for regulating and controlling the size of the plant organ; the AtGalOxs gene can be directly applied to plant type regulation and control of arabidopsis thaliana, and the homologous gene of the AtGalOxs gene has conservative property in crops such as cruciferae and the like, so that a key gene resource is provided for plant type improvement and yield increase of the crops, and the AtGalOxs gene has important significance in promoting development of a plant molecular breeding technology and realizing improvement of agricultural production benefits.