158 results about "Cytology" patented technology

The invention discloses a thyroid tumor diagnosis method and system based on ultrasonic and cytological image conjoint analysis. The method comprises the following steps: converting a thyroid B ultrasonic image of a patient to be diagnosed into an image feature vector FUS; performing structured feature extraction on the thyroid cytological image of the patient to be diagnosed to obtain a structured feature vector FCYTO; the image feature vector FUS and the structured feature vector FCYTO are converted into a fusion Token sequence; and inputting the Token into a multi-modal feature fusion and prediction network based on a Transform architecture, and carrying out feature fusion and classification prediction so as to obtain the probability that the thyroid tumor of the patient to be diagnosed is malignant. The thyroid tumor diagnosis based on multi-modal fusion is carried out on the basis of ultrasonic and cytological images, so that the diagnosis accuracy is improved.

The invention relates to the technical field of cytology, in particular to a grass carp brain astrocyte line and application thereof, the grass carp brain astrocyte line is preserved in China Center for Type Culture Collection on May 7, 2025, and the preservation number of the grass carp brain astrocyte line is CCTCC NO: C2025149. The grass carp brain astroglia cell line provided by the invention has the capability of efficiently proliferating GCRV-II, and the virus titer of the GCRV-II replicated in the cell line at least can reach 1.38 * 10 < 8 > pfu / mL or above; after the GCRV-II is blindly passed for 5 generations in a grass carp astroglia cell line, the grass carp can still have typical bleeding symptoms and death due to the virus. And the exogenous plasmid transfected grass carp brain astroglia cell line has similar transfection efficiency to commercial grass carp kidney cells. Therefore, the invention lays an important foundation for deep research of pathogenic mechanism of GCRV-II, vaccine preparation, antiviral drug screening and prevention and control of grass carp viral hemorrhagic disease.

The invention relates to the technical field of biology, in particular to a molecular marker for accurately positioning centromere of chromosome of Chinese trumpet creeper, and the sequence of the molecular marker is as shown in SEQ ID NO.1 and SEQ ID NO.2. The method comprises the following steps: specifically enriching a DNA (Deoxyribose Nucleic Acid) sequence combined with a centromere specific histone CENH3 through a chromatin co-immunoprecipitation technology (ChIP), and sequencing the DNA sequence; sequencing data are analyzed to obtain a centromere sequence of the vicia villosa, and two specific repetitive sequences of the centromere of the vicia villosa are obtained after identification and optimization. Primers are designed according to the sequences, PCR amplification is carried out, amplification product sequences are marked, fluorescence in situ hybridization verification is carried out by analyzing a cytogenetics technology, it is proved that the primers are specifically distributed in a centromere area of vicia villosa, and the centromere specificity of the primers is proved. The marker can be used for genetic identification and accurate positioning of the centromere in genome research, and can also be used for cytological identification of the centromere and karyotype research such as chromosome counting and morphological analysis through centromere signal observation.

The invention relates to the technical field of medical detection, in particular to a cervical cancer screening system based on risk HPV (human papillomavirus) expansion typing, and a risk assessment module provides solid technical support for individualized CIN2 + / 3 + risk assessment by using a Bayesian network and a Cox proportional risk model. By dynamically acquiring key data such as HPV types, cytological results, patient ages and infection duration, the Bayesian algorithm is beneficial to constructing individual specific risk models, and real-time risk division is carried out. The Cox regression model predicts accumulated risks in three years, dynamically adjusts a risk assessment threshold value and refreshes the weight of an input variable in real time in combination with a machine learning mechanism, and the composite model structure enables the risk assessment to be more flexible and ensures the scientificity of a management decision.

The invention belongs to the technical field of medical image processing, and provides a bone marrow cell WSI region-of-interest automatic analysis positioning method and system, and the method comprises the steps: obtaining a full glass slide bone marrow aspiration smear image (WSI); a pyramid network is adopted to zoom the full-glass-slide bone marrow aspiration smear image, and a low-resolution image is generated; designing a general area condition prompt strategy by combining bone marrow cytology medical priori knowledge, and calling a language model to construct text description of a bone marrow microparticle area for the low-resolution image; using a visual language model to align the text and the low-resolution image, and then adopting a feature visualization technology and based on gradient weighted channel selection to generate a semantic response graph; the dispersion degree of a semantic response region in the semantic response graph is calculated, and segmentation prompt information is generated by adopting a region perception and strategy self-adaption mechanism; and inputting segmentation prompt information and the low-resolution image into an SAM model, generating an ROI mask image through reasoning, and completing positioning of the bone marrow cell WSI region of interest.

A method for diagnosing nasal cytology based on deep learning includes: reading in blocks a nasal cytology to be diagnosed in a sliding window; performing preprocessing on the window image and inputting the preprocessed window image into a trained cell detection model, a coordinate and a score of a bounding box of a detected target being obtained based on feature maps, and a bounding box set being obtained by filtering out bounding boxes with scores below a predetermined threshold; cropping an image from the window image based on coordinates of bounding boxes in the bounding box set, performing preprocessing on the image and inputting the preprocessed image into a trained cell classification model to output a cell category of the corresponding image, and performing post-processing to obtain a cell category of the nasal cytology to be diagnosed.

The invention relates to the technical field of biological detection, and discloses a reagent composition and a kit for detecting cervical cancer and precancerous lesions of cervical cancer and application of the reagent composition and the kit. The reagent composition provided by the invention is used for detecting the methylation degree of a specific marker gene, and the detection sensitivity and specificity of cervical cancer and high-grade cervical cancer precancerous lesions are at a relatively high level. When the reagent composition is used for screening cervical cancer and precancerous lesions of HPV positive people, compared with conventional cytological detection, the colposcope referral rate is greatly reduced, meanwhile, the detection times for definite diagnosis of high-grade cervical lesions or cervical cancer are reduced, and the waste of medical resources and the examination burden of patients are reduced.

Provided is a microscopic layered image data analysis device for analyzing microscopic z-stack images for use in cytologic diagnosis. This microscopic layered image data analysis device for a specimen S containing a large number of cells comprises: a microscope (20) that acquires z-stack images of the specimen S; an imaging unit (30) that images the z-stack images acquired by the microscope (20); a storage unit (70) that stores the z-stack images captured by the imaging unit (30); and a control unit (50) that detects cells from the z-stack images stored in the storage unit (70), acquires a cell image group corresponding to each of the detected cells, crops a cell image from images forming the cell image group for each of the cells, acquires the confidence level of each of a plurality of class classifications for each of the cell images of the cells, and visualizes the confidence level acquired for each of the class classifications.

The invention relates to the technical field of biological detection, and discloses a reagent composition for detecting urothelial carcinoma, a kit and application. The composition provided by the invention is used for detecting the methylation state of a specific target sequence in a characteristic gene of the urinary tract epithelial cancer, and the detection sensitivity and specificity of the urinary tract epithelial cancer are at a relatively high level. The composition provided by the invention can be used for noninvasive urinary tract epithelium cancer screening, and when the composition is used for urinary tract epithelium cancer screening, compared with conventional urine cytology examination and iconography examination, the detection sensitivity is greatly improved, the early screening accuracy of urinary tract epithelium cancer is improved, and the misdiagnosis rate and the missed diagnosis rate are greatly reduced.

The invention discloses application of licochalcone as a drug sensitizer and an anti-tumor drug composition. The application comprises an application of the acanthochalcone in preparation of an anti-tumor drug sensitizer and an application of the acanthochalcone in improvement of the anti-tumor efficacy of platinum chemotherapeutic drugs, and the invention further provides an anti-tumor composition capable of efficiently inhibiting platinum drug-resistant gastric cancer cells based on the application. Through a gastric cancer organ model and an in-vitro cytology experiment, it is found that the acanthochalcone has an inhibiting effect on proliferation of gastric cancer cells, and the sensitivity of the gastric cancer cells to platinum drugs can be remarkably enhanced. The invention also finds that IC50 of gastric cancer cells to cis-platinum and oxaliplatin, especially platinum acquired drug-resistant gastric cancer cells, can be significantly reduced by combined use of the acanthochalcone and platinum drugs. Therefore, the invention provides a new medication basis and choice for overcoming acquired platinum drug resistance of gastric cancer by utilizing the acanthochalcone.

The utility model discloses a liquid-based cell preservation bottle, which belongs to the technical field of medical equipment and comprises a bottle body for containing preservation liquid and a cover body for sealing the bottle body, the bottle body is hermetically connected with the cover body, a through hole is formed in the top surface of the cover body, the through hole is communicated with the bottle body, a sealing cover is arranged on the cover body, a rubber sealing plug is arranged on the sealing cover, and the rubber sealing plug is communicated with the bottle body. The rubber sealing plug is arranged in the through hole and tightly attached to the inner wall of the through hole. The device has the effects of facilitating operation of workers and improving the working efficiency of the workers.

The invention discloses equipment for preparing cytological specimens, which comprises a shell and a centrifugal turntable rotationally arranged in the shell, a centrifugal frame is rotationally arranged on the centrifugal turntable, and a centrifugal tube is mounted in the centrifugal frame. A hole is formed in the bottom of the centrifugal tube, and a rubber plug is arranged in the hole; a material taking device is arranged in the shell, a material taking needle tube is arranged on the side, facing the rubber plug, of the material taking device, and an air cylinder used for pushing the material taking device to move in the direction of the centrifugal tube is further arranged in the shell. After centrifugation is finished, the cell sample at the bottommost part of the centrifugal tube can be directly extracted without inclining the centrifugal tube. Therefore, shaking of the centrifugal tube after centrifugation is reduced, and liquid mixing after centrifugal layering is reduced. Therefore, the purity of subsequent smears is improved.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The invention belongs to the technical field of plant cytology, and particularly relates to a method for improving the transformation efficiency of plant protoplast, which comprises the following steps: preparing enzymatic hydrolysate: each liter of enzymatic hydrolysate contains 20 mmol of KCl, 5-20 g of cellulase, 2-6 g of macerozyme, 10 mmol of CaCl2, 1 g of BSA and the balance of water; performing leaf enzymolysis; obtaining a protoplast; preparing a competent protoplast; resuspending the MMG solution; and transforming and incubating the protoplast resuspension by using the target plasmid. According to the method, the high-quality protoplast can be obtained, and when the protoplast is applied to downstream experiments, the conversion efficiency and stability of the experiments can be remarkably improved.

The present invention relates to a computer-implemented method for producing a trained model for detecting one or more defined cell types and / or cellular indicators, such as biomarkers or cellular abnormalities, in particular genomic aberrations, from single-cell images obtained from a sample of a body liquid, bone marrow, cytology smear or slide preparations, in particular a blood smear sample. It relates also to a computer-implemented method for detecting one or more defined cell types and / or cellular indicators, such as biomarkers or cellular abnormalities, in particular genomic aberrations, from single-cell images obtained from a sample of a body liquid, bone marrow or cytology smear, in particular a blood smear sample.

The invention relates to a compound as shown in a formula (I) and / or a formula (II) and / or a formula (III) or a tautomer or pharmaceutically acceptable salt thereof, a preparation method and application of the compound or the tautomer or the pharmaceutically acceptable salt in medicine. Specifically, the fluorescent probe can be used as a fluorescent probe for cytological imaging, can also be used as a near-infrared fluorescent imaging probe for diagnosing benign / malignant lesions such as fibrosis, nodules and tumors, and can also be applied to research of related biological events in cytobiology and molecular biology. The fluorescent probe can be particularly applied to fluorescent navigation in tumor resection surgery.

The invention discloses a method and device for identifying helicobacter pylori based on artificial intelligence fluorescence imaging, and relates to the technical field of artificial intelligence medical detection. Comprising the following steps: S1, carrying out slide preparation treatment on a cytological sample or a tissue slice sample, and processing the slide preparation through an immunofluorescent staining reagent to obtain a helicobacter pylori fluorescent staining picture; s2, outputting and obtaining a helicobacter pylori picture with a rectangular frame identifier through a helicobacter pylori identifier neural network model; and S3, according to the coordinate information of the rectangular frame, obtaining the coordinate information of the helicobacter pylori in the fluorescent staining picture, and splicing the helicobacter pylori pictures to obtain a spliced picture. According to the method, the helicobacter pylori is marked automatically through the helicobacter pylori identification neural network model, the problems of subjectivity and time consumption of traditional manual microscopic examination are avoided, meanwhile, the positions of thalli are accurately marked, positive probability data are output, and then the detection efficiency is improved.

Provided are a centromere protein CENP-M small molecule inhibitor cenpemlin, and a preparation method therefor and the use thereof. A small molecule compound cenpemlin is obtained by means of analyzing the protein plane information of the fine structures of CENP-M and CENP-L and performing targeted screening. The results of biochemical and cytological experiments all indicate that cenpemlin inhibits the proliferation of cancer cells by means of interfering with the interaction between CENP-M and CENP-L, thereby providing a new compound for analyzing and interfering with the rapid proliferation of cancer cells.

The invention relates to the technical field of liquid-based cytology, and particularly discloses serosa effusion cell preserving fluid. Comprising a preservation solution, an anticoagulant, a red blood cell treatment solution, a cell membrane stabilizer, an antioxidant and a metal ion chelating agent, the preservation solution contains a buffer system, an energy supply component, an antibiotic and a surfactant, and the red blood cell treatment solution contains a hemolysis active component and a cell immobilization component; the preparation method comprises the following steps: firstly, preparing the preserving fluid, adjusting the pH value, adding the active substances, mixing the antibiotics, and then completing constant volume and sterile filtration; the anticoagulant solution and the red blood cell treating fluid are independently prepared, and sterilization is achieved through filtration; and finally, packaging all the components in a sterilized storage bottle in a matched manner. The composition provided by the invention can be used for preserving cells in a clinical serosal effusion sample, and can provide a high-quality sample for subsequent cell morphology observation or detection; in addition, according to the preparation method, the controllability is high when all the components are independently prepared, and the preserving fluid with the consistent performance can be stably prepared.

The present invention relates to the field of medicine, and specifically to the use of taraxasterol or its salts in the preparation of drugs for preventing or treating traumatic brain injury. Animal experiments have confirmed that the taraxasterol of the present invention can improve the motor ability of the balance beam experiment in the neurological function score of mice with brain trauma, improve the neurological function scores including motor tests and sensory tests; reduce the permeability of the blood-brain barrier, reduce brain edema, improve the short-term lesion volume after traumatic brain injury, and inhibit microglial activation and immune cell infiltration. Cytological experiments have confirmed that taraxasterol can reduce microglia-mediated neuroinflammation, reduce the expression of inflammatory factors such as TNF-α, IL-6, and IL-1β, and reduce neuronal damage.

The invention discloses an application of a novel phosphodiesterase 4 (PDE4) inhibitor ZL-n-91 in preparation of drugs for treating growth and metastasis of small cell lung cancer. In-vitro cytology experiments and in-vivo zoology experiments show that the phosphodiesterase 4 inhibitor ZL-n-91 can significantly inhibit proliferation and metastasis of small cell lung cancer, and the phosphodiesterase 4 inhibitor ZL-n-91 is expected to treat small cell lung cancer and has good development and application prospects.

The application discloses a marker group for cervical cancer screening and diagnosis and application thereof, and belongs to the field of molecular biological technology.The marker group comprises at least four polypeptides shown in sequences of SEQ ID NO.1-42.The marker group constructed by the application has more excellent sensitivity and specificity when used for auxiliary diagnosis and early screening of cervical cancer.The auxiliary diagnosis result is superior to the efficiency (sensitivity 81.1%, specificity 78.9%) of combined detection of serum CEA, SCC-Ag and CA125 in early diagnosis of cervical cancer;the diagnostic sensitivity and specificity of serum cf-DNA are 72.2% and 81.7% respectively.The early screening result is also superior to the internationally recognized cervical cancer screening method, i.e., combined screening of cytological detection (TCT) and human papilloma virus (HPV) virology detection.

The application relates to the field of cytology and relates to an oligonucleotide probe set design for identifying Brassica rapa chromosomes and application, which comprises the following steps: S1: obtaining a NR-TR set in a Brassica rapa R-o-18 reference genome; S2: statistically clustering the NR-TR set and obtaining TR array data of Brassica rapa chromosomes; S3: obtaining a repetitive sequence cluster which is enriched in all the chromosomes of Brassica rapa; S4: segmenting a repetitive sequence representative array of the obtained repetitive sequence cluster; and S5: respectively aligning the segmented sequences to the Brassica rapa R-o-18 reference genome by using blastn. The application establishes a technology for coating Brassica rapa chromosomes, and the technology can batch coat Brassica rapa chromosome materials.

The invention discloses a biological additive for promoting poultry feather development and application thereof, and belongs to the technical field of animal breeding. The biological additive is prepared from scallop skirt oligopeptide and stiff silkworm polypeptide according to the mass ratio of 1: 1. Animal experiments show that when 60 mg of the biological additive is added into every 1 kg of basic feed to feed broilers, the growth of the broilers can be promoted, and more importantly, the feather development of the broilers can be remarkably and synergistically promoted, and the length of main wing feather is obviously increased. The cytology research further proves that the biological additive can synergistically promote the proliferation of the poultry hair follicle epidermal stem cells, and the effect of the biological additive is superior to that of EGF positive control. Therefore, the biological additive developed by the invention is safe, efficient and green, effectively solves the problems of drug residue and drug resistance possibly caused by the traditional chemical additive, provides a sustainable solution for poultry breeding, and has important economic and application values.

A computer-implemented method for cytological grading includes receiving (302, 402) first image data (102) representing a biological sample, and parsing (304, 404) the received first image data into a plurality of patches (104). Each of the plurality of patches represents a corresponding portion of the received first image data. The method further includes identifying (306, 406) at least one cytological feature of each of the plurality of patches (108) using a first trained machine learning model (106), determining (308, 408) at least one statistic (110) based on the identified at least one cytological feature of each of the plurality of patches using a second trained machine learning model, and outputting (310, 410) the determined at least one statistic.

The invention relates to the technical field of preparation of composite scaffolds, in particular to a preparation method of a novel photosensitive injectable CMCSMA composite bioactive hydrogel scaffold loaded with urine-derived stem cell subpopulation exosomes, and the preparation method comprises the following steps: S1, preparing reagents and instruments; s2, extracting and culturing urine-derived stem cells; s3, carrying out flow cytometry identification and subgroup sorting on the urine-derived stem cells, wherein the subgroup sorting is based on CD133 and CD271 double-positive cell surface markers; s4, extracting and storing the exosome; s5, synthesizing methacrylated carboxymethyl chitosan; and S6, mixing the extracted exosome with a methacrylated carboxymethyl chitosan solution, so as to prepare the CMCSMA-sExos composite bioactive hydrogel scaffold. According to the invention, the exosome is loaded by the methacrylated carboxymethyl chitosan hydrogel, so that slow release and targeted delivery of the exosome are realized, and the in-vivo action time of the exosome is prolonged.

The present invention relates to a method for providing information for predicting the likelihood of bladder cancer recurrence, which provides information that patients who have undergone transurethral resection of a bladder tumor and test positive for suspicious high-grade urothelial carcinoma (SHGUC) or high-grade urothelial carcinoma (HGUC) in a urine cytology test have a higher likelihood of bladder cancer recurrence compared with patients who do not test positive.

The application discloses a biological additive for promoting poultry feather development and application thereof, and belongs to the technical field of animal breeding. The biological additive is prepared from scallop skirt oligopeptide and cantharides polypeptide at a mass ratio of 1:1. Animal experiments show that, when meat chickens are fed with 60 mg of the biological additive per 1 kg of basic feed, the growth of the meat chickens can be promoted, and more importantly, the feather development of the meat chickens can be significantly and synergistically promoted, and the length of the main wing feathers is obviously increased. Cytological studies further prove that the biological additive can synergistically promote the proliferation of poultry hair follicle epidermal stem cells, and the effect is better than that of an EGF positive control. Therefore, the biological additive developed by the application is safe, efficient and green, effectively solves the problems of drug residues and drug resistance possibly caused by traditional chemical additives, provides a sustainable solution for poultry breeding, and has important economic and application values.

The invention provides a computer-implemented method for assisting diagnosis of dysplasia from a liquid-based cytology whole-slide image (WSI), using a multi-stage pipeline combining deep- learning-based detection with engineered feature extraction and a calibrated machine-learning classifier. The method involves receiving and resampling a digital WSI to a canonical spatial resolution, detecting individual cell instances with a neural network, computing morphological and texture features for each detected cell, aggregating these features across the WSI to form a slide-level feature vector, inputting this vector into a calibrated classifier to yield a slide-level probability of dysplasia, computing slide-quality metrics to govern downstream triage, presenting results via an interactive graphical interface with per-cell thumbnails, and iteratively refining the system with expanded and harmonized training data from expert annotations.