245 results about "Cytology" patented technology

The invention discloses a thyroid tumor diagnosis method and system based on ultrasonic and cytological image conjoint analysis. The method comprises the following steps: converting a thyroid B ultrasonic image of a patient to be diagnosed into an image feature vector FUS; performing structured feature extraction on the thyroid cytological image of the patient to be diagnosed to obtain a structured feature vector FCYTO; the image feature vector FUS and the structured feature vector FCYTO are converted into a fusion Token sequence; and inputting the Token into a multi-modal feature fusion and prediction network based on a Transform architecture, and carrying out feature fusion and classification prediction so as to obtain the probability that the thyroid tumor of the patient to be diagnosed is malignant. The thyroid tumor diagnosis based on multi-modal fusion is carried out on the basis of ultrasonic and cytological images, so that the diagnosis accuracy is improved.

The invention relates to the technical field of cytology, in particular to a grass carp brain astrocyte line and application thereof, the grass carp brain astrocyte line is preserved in China Center for Type Culture Collection on May 7, 2025, and the preservation number of the grass carp brain astrocyte line is CCTCC NO: C2025149. The grass carp brain astroglia cell line provided by the invention has the capability of efficiently proliferating GCRV-II, and the virus titer of the GCRV-II replicated in the cell line at least can reach 1.38 * 10 < 8 > pfu / mL or above; after the GCRV-II is blindly passed for 5 generations in a grass carp astroglia cell line, the grass carp can still have typical bleeding symptoms and death due to the virus. And the exogenous plasmid transfected grass carp brain astroglia cell line has similar transfection efficiency to commercial grass carp kidney cells. Therefore, the invention lays an important foundation for deep research of pathogenic mechanism of GCRV-II, vaccine preparation, antiviral drug screening and prevention and control of grass carp viral hemorrhagic disease.

The invention relates to the technical field of biology, in particular to a molecular marker for accurately positioning centromere of chromosome of Chinese trumpet creeper, and the sequence of the molecular marker is as shown in SEQ ID NO.1 and SEQ ID NO.2. The method comprises the following steps: specifically enriching a DNA (Deoxyribose Nucleic Acid) sequence combined with a centromere specific histone CENH3 through a chromatin co-immunoprecipitation technology (ChIP), and sequencing the DNA sequence; sequencing data are analyzed to obtain a centromere sequence of the vicia villosa, and two specific repetitive sequences of the centromere of the vicia villosa are obtained after identification and optimization. Primers are designed according to the sequences, PCR amplification is carried out, amplification product sequences are marked, fluorescence in situ hybridization verification is carried out by analyzing a cytogenetics technology, it is proved that the primers are specifically distributed in a centromere area of vicia villosa, and the centromere specificity of the primers is proved. The marker can be used for genetic identification and accurate positioning of the centromere in genome research, and can also be used for cytological identification of the centromere and karyotype research such as chromosome counting and morphological analysis through centromere signal observation.

The invention relates to the technical field of medical detection, in particular to a cervical cancer screening system based on risk HPV (human papillomavirus) expansion typing, and a risk assessment module provides solid technical support for individualized CIN2 + / 3 + risk assessment by using a Bayesian network and a Cox proportional risk model. By dynamically acquiring key data such as HPV types, cytological results, patient ages and infection duration, the Bayesian algorithm is beneficial to constructing individual specific risk models, and real-time risk division is carried out. The Cox regression model predicts accumulated risks in three years, dynamically adjusts a risk assessment threshold value and refreshes the weight of an input variable in real time in combination with a machine learning mechanism, and the composite model structure enables the risk assessment to be more flexible and ensures the scientificity of a management decision.

Disclosed is a computer-implemented method for real-time classification of cells in a body tissue that includes capturing one or more images of a cytology slide sample of the body tissue, pre-processing the captured one or more images, detecting and extracting a plurality of regions of interest in the pre-processed images using a cell detection process, wherein each region of interest includes one or more cells or cell clusters of interest, segmenting each individual cell or cluster in the regions of interest and extracting pixel content of each segmented cell or cluster, extracting semantic features from each segmented cell image using an embedding extraction model, classifying each individual cell or cell cluster based on corresponding extracted sematic features, and predicting a class of the cytology slide sample, based on one or more positions and relationships among one or more classified cells, using a slide level decision model.

The invention discloses an application of ansamitocin P-3 (Ansamitocin P-3) in preparation of a medicine for treating gastric cancer. The invention proves that the ansamitocin P-3 enables gastric cancer cells to die by influencing the apoptosis pathway of the cells through cytological research. Meanwhile, a stomach cancer abdominal cavity planting model of a nude mouse is constructed, and it is also observed that the ansamitocin P-3 has a killing effect on stomach cancer in the model. The invention provides a new application of ansamitocin P-3 in treatment of gastric cancer, and the ansamitocin P-3 has good clinical transformation value. The invention provides a new treatment means for gastric cancer patients with drug resistance.

The invention discloses a double-channel gold-copper-doped graphene electrochemical immunosensor as well as a preparation method and application thereof. The double-channel gold-copper-doped graphene electrochemical immunosensor comprises a gold / gold-copper co-doped vertical graphene electrode, and bovine serum albumin and an antibody which are incubated on the gold / gold-copper co-doped vertical graphene electrode, the gold / gold-copper co-doped vertical graphene electrode comprises a gold-copper co-doped vertical graphene electrode and gold nanoparticles loaded on the surface of the gold-copper co-doped vertical graphene electrode, and the gold-copper co-doped vertical graphene electrode comprises a substrate and a gold-copper co-doped vertical graphene nanosheet grown on the surface of the substrate. The sensor capable of sensitively detecting the concentration of the carcinoembryonic antigen secreted by the tumor cells is prepared, an immunoreaction detection system is built, a reliable industrial signal amplification platform is provided for early diagnosis of tumors, and a new strategy is developed for research of tumor cytology.

The invention belongs to the technical field of medical image processing, and provides a bone marrow cell WSI region-of-interest automatic analysis positioning method and system, and the method comprises the steps: obtaining a full glass slide bone marrow aspiration smear image (WSI); a pyramid network is adopted to zoom the full-glass-slide bone marrow aspiration smear image, and a low-resolution image is generated; designing a general area condition prompt strategy by combining bone marrow cytology medical priori knowledge, and calling a language model to construct text description of a bone marrow microparticle area for the low-resolution image; using a visual language model to align the text and the low-resolution image, and then adopting a feature visualization technology and based on gradient weighted channel selection to generate a semantic response graph; the dispersion degree of a semantic response region in the semantic response graph is calculated, and segmentation prompt information is generated by adopting a region perception and strategy self-adaption mechanism; and inputting segmentation prompt information and the low-resolution image into an SAM model, generating an ROI mask image through reasoning, and completing positioning of the bone marrow cell WSI region of interest.

The invention belongs to the technical field of biological protein, and particularly relates to a cytological detection method of recombinant three-type humanized collagen, which comprises the following steps: S1, culturing cells: inoculating human dermal fibroblasts into a culture medium, and culturing to a logarithmic phase for later use; s2, co-culture: co-culturing the standard recombinant three-type humanized collagen with different concentrations and the human dermal fibroblasts in the logarithmic production period; s3, constructing a standard curve; s4, quantifying and evaluating. The concentration of the target protein can be calculated according to the standard curve, the method is simple, expensive detection instruments are not needed, and the method is more practical and convenient. Meanwhile, dozens of samples can be detected in batches at the same time, and the detection efficiency is greatly improved. When the concentration of the target protein is quantitatively detected, the biological activity of the target protein can be evaluated through the cell adhesion rate, and compared with an existing detection method, more biological information of the target protein can be provided.

The invention belongs to the technical field of biological medicine, and particularly relates to a construction method of a gastric mucosa intestinal epithelial metaplasm type organ model with goblet cell phenotype. The model is characterized by MUC2 + MUC6 + goblet cells, and histological characteristics of gastric mucosa intestinal metaplasia are more deeply simulated from the aspect of cell phenotype. The construction method comprises the following steps: (1) obtaining a human stomach gland to prepare an organoid; (2) subculturing the organoid; (3) construction of a pCDH-CMV-MCS-EF1-CDX2-Puro overexpression lentiviral vector, and construction of a pCDH- (4) virus packaging; (5) constructing CDX2 overexpression stably transfected cells and organoid; and (6) carrying out gradient differentiation on the CDX2 overexpression organoid to prepare the intestinal metaplasia organoid. According to the invention, the pathological transformation of the cytology level in the gastrointestinal mucosa intestinal epithelial metaplasia process is realized in the in-vitro model for the first time, and a new research model and theoretical guidance are provided for the diagnosis, prevention and individualized precise treatment of precancerous lesions of gastric cancer.

The invention discloses a high-activity recombinant human XVII type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The recombinant human XVII type collagen comprises 138 amino acids (SEQ ID NO: 1) of an XVII type collagen functional area and 15 amino acids (SEQ ID NO: 2) of a cell binding structural domain of I type collagen, and the amino acid sequences of the recombinant human XVII type collagen are further mutated to obtain the high-activity recombinant human XVII type collagen. The invention also provides an expression vector construction method of the protein and a preparation method of the recombinant human XVII type collagen. The theoretical molecular weight of the protein is small and is 14.6 kDa, and cytological experiments show that the protein has high biological activity for promoting cell migration, proliferation and adhesion and can be used for research, development and application of medical instruments and biological skin care products.

A method for diagnosing nasal cytology based on deep learning includes: reading in blocks a nasal cytology to be diagnosed in a sliding window; performing preprocessing on the window image and inputting the preprocessed window image into a trained cell detection model, a coordinate and a score of a bounding box of a detected target being obtained based on feature maps, and a bounding box set being obtained by filtering out bounding boxes with scores below a predetermined threshold; cropping an image from the window image based on coordinates of bounding boxes in the bounding box set, performing preprocessing on the image and inputting the preprocessed image into a trained cell classification model to output a cell category of the corresponding image, and performing post-processing to obtain a cell category of the nasal cytology to be diagnosed.

The invention provides an integrated cervical HPV (human papillomavirus) and cervical cytology self-sampling device, and belongs to the field of medical instruments. The problems that most existing cervix uteri self-sampling devices are designed in a split mode, samplers and specimen protection liquid are packaged separately, the structure is complex, large-scale production is not facilitated, the sampling efficiency of sampling heads is low, and samples of the sampling heads are prone to being polluted are solved. The device comprises a sleeve, a central sampling tube and a balloon, the sleeve slidably sleeves the outer side of the central sampling tube, the central sampling tube is of a hollow structure, the front end of the central sampling tube is provided with a sampling head, the outer side of the central sampling tube is fixedly sleeved with a piston, the outer wall of the piston is in contact with the inner wall of the sleeve and is in sliding fit with the inner wall of the sleeve, and the outer wall of the piston is in sliding fit with the inner wall of the sleeve. The tail end of the central sampling tube is connected with a balloon, a plurality of through holes are formed in the side wall of the central sampling tube between the sampling head and the piston, and a sealing end cover is arranged at the front end of the sleeve. The self-sampling device is mainly used for self-sampling of cervical cancer screening.

The invention relates to an intracerebral phosphorus homeostasis accelerant and application thereof. Specifically, a gene compensation therapy for targeting glial cells, especially astrocytes, is developed on the basis of a molecular cytology mechanism of the intracerebral phosphorus homeostasis, and can be used for repairing the intracerebral phosphorus homeostasis imbalance condition, so that the occurrence of calcification is inhibited, or the further progress of intracerebral calcification is inhibited, and the intracerebral phosphorus homeostasis is inhibited. And clinical nervous system symptoms of related patients are improved.

The invention relates to the technical field of biological detection, and discloses a reagent composition and a kit for detecting cervical cancer and precancerous lesions of cervical cancer and application of the reagent composition and the kit. The reagent composition provided by the invention is used for detecting the methylation degree of a specific marker gene, and the detection sensitivity and specificity of cervical cancer and high-grade cervical cancer precancerous lesions are at a relatively high level. When the reagent composition is used for screening cervical cancer and precancerous lesions of HPV positive people, compared with conventional cytological detection, the colposcope referral rate is greatly reduced, meanwhile, the detection times for definite diagnosis of high-grade cervical lesions or cervical cancer are reduced, and the waste of medical resources and the examination burden of patients are reduced.

The invention discloses recombinant laminin with anti-aging and repairing effects and a preparation method of the recombinant laminin, relates to the technical field of recombinant proteins, and aims to solve the technical problems that existing laminin is relatively low in yield and limited in application. According to the recombinant laminin with the anti-aging and repairing effects, the sequence of the recombinant laminin is shown as SEQ ID No.3, the total number is 363aa, and the predicted molecular weight is 40.4 kD. Compared with the prior art, the recombinant laminin is synthesized by embedding a laminin gene sequence into a pichia pastoris genome for heterologous expression. A cytological detection method for evaluating the efficacy of a skin care product is applied, and a research result shows that the recombinant laminin can remarkably promote cell adhesion and migration activity and can stimulate cells to generate collagen, so that the anti-aging and repairing functions of the recombinant laminin are shown.

The invention discloses a corn kernel development regulation gene ZmRH51 as well as an encoding protein, a functional marker and an application of the corn kernel development regulation gene ZmRH51. According to the application, a corn kernel mutant m223 is taken as a basic material, heterozygote of the corn kernel mutant m223 is hybridized with Zheng 58 (Z58) to construct an F2 segregation population, then phenotype identification and cytological observation are performed on the segregation population of the offspring, the gene ZmRH51 for controlling the mutation phenotype of the m223 is obtained through genetic analysis, BSR-seq analysis and map-based cloning, the gene is positioned in a nucleolus region, and the mutation phenotype of the m223 is controlled. The ZmRH51 is used for coding RNA helicase of a DEAD-Box gene family, and research shows that ZmRH51 participates in ribosome assembly and can influence processing of pre-rRNA in corn kernels; the excellent allelic variation genotype of the excavated ZmRH51 gene in the aspect of grain thickness is obtained through further excavation, the grain thickness and hundred-grain weight of the ZmRH51 gene can be increased through the utilization of the gene, and then the yield is increased. In addition, distribution and utilization potentials of different haplotypes in a selfing line are researched, and technical support and excellent germplasm resources are provided for utilization of the gene.

The invention relates to the technical field of medicines, and provides application of ibrutinib as a platinum drug sensitizer in gastric cancer treatment. Furthermore, the invention also provides an application of ibrutinib combined with platinum drugs in preparation of drugs for treating CREB1 high-expression gastric cancer, and further provides a pharmaceutical composition for treating gastric cancer. A mouse in-vivo experiment shows that the size and the growth speed of a stomach tumor overexpressed with CREB1 can be obviously reduced by combined use of ibrutinib and oxaliplatin, so that the platinum drug sensitivity of CREB1 high-expression stomach cancer cells is enhanced; through a gastric cancer organ model and an in-vitro cytology experiment, the ibrutinib can reduce the activity of CREB1 by inhibiting phosphorylation of various proteins of an EGFR-PI3K-AKT-CREB1 pathway, so that proliferation of gastric cancer cells is inhibited, the ferroptosis level of the gastric cancer cells is increased, and IC50 of the ibrutinib can be remarkably reduced by combined use of the ibrutinib and platinum drugs. The invention provides a new basis for the ibrutinib or the derivative thereof to inhibit the growth of CREB1 high-expression gastric cancer cells and enhance the sensitivity of platinum drugs.

Provided is a microscopic layered image data analysis device for analyzing microscopic z-stack images for use in cytologic diagnosis. This microscopic layered image data analysis device for a specimen S containing a large number of cells comprises: a microscope (20) that acquires z-stack images of the specimen S; an imaging unit (30) that images the z-stack images acquired by the microscope (20); a storage unit (70) that stores the z-stack images captured by the imaging unit (30); and a control unit (50) that detects cells from the z-stack images stored in the storage unit (70), acquires a cell image group corresponding to each of the detected cells, crops a cell image from images forming the cell image group for each of the cells, acquires the confidence level of each of a plurality of class classifications for each of the cell images of the cells, and visualizes the confidence level acquired for each of the class classifications.

The invention relates to the technical field of biological detection, and discloses a reagent composition for detecting urothelial carcinoma, a kit and application. The composition provided by the invention is used for detecting the methylation state of a specific target sequence in a characteristic gene of the urinary tract epithelial cancer, and the detection sensitivity and specificity of the urinary tract epithelial cancer are at a relatively high level. The composition provided by the invention can be used for noninvasive urinary tract epithelium cancer screening, and when the composition is used for urinary tract epithelium cancer screening, compared with conventional urine cytology examination and iconography examination, the detection sensitivity is greatly improved, the early screening accuracy of urinary tract epithelium cancer is improved, and the misdiagnosis rate and the missed diagnosis rate are greatly reduced.

The invention discloses application of licochalcone as a drug sensitizer and an anti-tumor drug composition. The application comprises an application of the acanthochalcone in preparation of an anti-tumor drug sensitizer and an application of the acanthochalcone in improvement of the anti-tumor efficacy of platinum chemotherapeutic drugs, and the invention further provides an anti-tumor composition capable of efficiently inhibiting platinum drug-resistant gastric cancer cells based on the application. Through a gastric cancer organ model and an in-vitro cytology experiment, it is found that the acanthochalcone has an inhibiting effect on proliferation of gastric cancer cells, and the sensitivity of the gastric cancer cells to platinum drugs can be remarkably enhanced. The invention also finds that IC50 of gastric cancer cells to cis-platinum and oxaliplatin, especially platinum acquired drug-resistant gastric cancer cells, can be significantly reduced by combined use of the acanthochalcone and platinum drugs. Therefore, the invention provides a new medication basis and choice for overcoming acquired platinum drug resistance of gastric cancer by utilizing the acanthochalcone.

The utility model discloses a liquid-based cell preservation bottle, which belongs to the technical field of medical equipment and comprises a bottle body for containing preservation liquid and a cover body for sealing the bottle body, the bottle body is hermetically connected with the cover body, a through hole is formed in the top surface of the cover body, the through hole is communicated with the bottle body, a sealing cover is arranged on the cover body, a rubber sealing plug is arranged on the sealing cover, and the rubber sealing plug is communicated with the bottle body. The rubber sealing plug is arranged in the through hole and tightly attached to the inner wall of the through hole. The device has the effects of facilitating operation of workers and improving the working efficiency of the workers.

The invention relates to a cell cleaning solution, a reagent combination for serosal cavity liquid-based cell treatment, a serosal cavity liquid-based cell staining kit and a staining method, and belongs to the technical field of biological detection. In order to consider the morphology and the ICC dyeing effect, the invention provides a formula of the cell cleaning fluid based on a large number of experimental studies in the early stage, and subsequent experiments prove that when the cell cleaning fluid is used for treating a bloody specimen, the effects of rapid hemolysis, mucus and protein fluid removal and the like can be realized, and meanwhile, other cells are effectively fixed; the morphological integrity and good antigenicity of other cells are ensured. And a thin and uniform cell layer is prepared through natural sedimentation, is suitable for cytological detection, has a clear background, and can completely show the morphology and structure of cells. The cell cleaning fluid is a medium for cleaning and dissolving red blood cells, can generate a destructive effect on the red blood cells, and is an effective reagent capable of ensuring the morphological integrity and good antigenicity of other cells.

The invention discloses equipment for preparing cytological specimens, which comprises a shell and a centrifugal turntable rotationally arranged in the shell, a centrifugal frame is rotationally arranged on the centrifugal turntable, and a centrifugal tube is mounted in the centrifugal frame. A hole is formed in the bottom of the centrifugal tube, and a rubber plug is arranged in the hole; a material taking device is arranged in the shell, a material taking needle tube is arranged on the side, facing the rubber plug, of the material taking device, and an air cylinder used for pushing the material taking device to move in the direction of the centrifugal tube is further arranged in the shell. After centrifugation is finished, the cell sample at the bottommost part of the centrifugal tube can be directly extracted without inclining the centrifugal tube. Therefore, shaking of the centrifugal tube after centrifugation is reduced, and liquid mixing after centrifugal layering is reduced. Therefore, the purity of subsequent smears is improved.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The invention belongs to the technical field of plant cytology, and particularly relates to a method for improving the transformation efficiency of plant protoplast, which comprises the following steps: preparing enzymatic hydrolysate: each liter of enzymatic hydrolysate contains 20 mmol of KCl, 5-20 g of cellulase, 2-6 g of macerozyme, 10 mmol of CaCl2, 1 g of BSA and the balance of water; performing leaf enzymolysis; obtaining a protoplast; preparing a competent protoplast; resuspending the MMG solution; and transforming and incubating the protoplast resuspension by using the target plasmid. According to the method, the high-quality protoplast can be obtained, and when the protoplast is applied to downstream experiments, the conversion efficiency and stability of the experiments can be remarkably improved.

The present invention concerns a computer-implemented method for detecting one or more defined genomic aberrations from an image of a body liquid, bone marrow or cytological sample, for instance a cytology smear sample, in particular a blood smear sample.

Automated cell staining systems and methods are disclosed herein. In particular, the staining systems disclosed herein provide low-volume, automated bench top staining systems for staining biological samples contained on a cytological slide.

The utility model relates to an automatic tool setting device for a wax block of a slicing machine, which belongs to medical laboratory equipment and is applied to the fields of medical clinical pathological histology, cytology, clinical examination, biological research and the like. The wax block automatic tool setting device has the advantages that the displacement sensor is installed in the middle of the side of the wax block chuck below the blade of the blade frame, automatic tool setting of the wax block is achieved through positioning signals and control of the displacement sensor, fingers are prevented from being cut or scratched in the manual tool setting process of the wax block, the accuracy of tool setting of the wax block is improved, and the speed of tool setting of the wax block is increased; and the practicability, flexibility and reliability are improved. Unnecessary manual operation steps are reduced, simplicity and rapidness are achieved, labor and time are saved, and the working efficiency is improved. Therefore, the method has great market prospect and economic value.