26 results about "Cytology" patented technology

The present invention relates to a computer-implemented method for producing a trained model for detecting one or more defined cell types and / or cellular indicators, such as biomarkers or cellular abnormalities, in particular genomic aberrations, from single-cell images obtained from a sample of a body liquid, bone marrow, cytology smear or slide preparations, in particular a blood smear sample. It relates also to a computer-implemented method for detecting one or more defined cell types and / or cellular indicators, such as biomarkers or cellular abnormalities, in particular genomic aberrations, from single-cell images obtained from a sample of a body liquid, bone marrow or cytology smear, in particular a blood smear sample.

A computer-implemented method for cytological grading includes receiving (302, 402) first image data (102) representing a biological sample, and parsing (304, 404) the received first image data into a plurality of patches (104). Each of the plurality of patches represents a corresponding portion of the received first image data. The method further includes identifying (306, 406) at least one cytological feature of each of the plurality of patches (108) using a first trained machine learning model (106), determining (308, 408) at least one statistic (110) based on the identified at least one cytological feature of each of the plurality of patches using a second trained machine learning model, and outputting (310, 410) the determined at least one statistic.

This invention belongs to the field of plant breeding and genetic engineering technology, specifically involving AtGalOxs Genes and their application in regulating plant organ size and development. Specifically, this invention screened a gene that regulates plant organ size and development. AtGalOxs It was identified as a key gene regulating the size and development of organs in Arabidopsis thaliana plants, and this was confirmed through cytological observation and transcriptome analysis. AtGalOxs Genes function by regulating cell proliferation and auxin signaling pathways, providing new theoretical basis for elucidating the molecular network regulating plant organ size; AtGalOxs The gene can be directly applied to the regulation of Arabidopsis plant architecture, and its homologous genes are conserved in crops such as Brassicaceae. This provides key gene resources for improving crop plant architecture and increasing yield, which is of great significance for promoting the development of plant molecular breeding technology and improving agricultural production efficiency.

The present invention relates to a computer-implemented method for producing a trained model for detecting one or more defined cell types and / or cellular indicators, such as biomarkers or cellular abnormalities, in particular genomic aberrations, from single-cell images obtained from a sample of a body liquid, bone marrow, cytology smear or slide preparations, in particular a blood smear sample. It relates also to a computer-implemented method for classifying a sample of a body liquid, bone marrow or cytology smear, in particular a blood smear sample, as indicative or non-indicative of one or more defined cell types and / or cellular indicators, such as biomarkers or cellular abnormalities, in particular genomic aberrations, from single-cell images obtained from the sample.

This invention discloses a KRAS protein degrader modified with norbornene as a hydrophobic group, its preparation method, and its applications. This invention provides a protein degrader with norbornene as the hydrophobic group and MRTX1133 as the KRAS ligand terminus, capable of selectively binding to KRAS (preferably the G12D mutant) and inducing its degradation within cells through a hydrophobic tag, thereby inhibiting downstream oncogenic signaling pathways and suppressing tumor cell proliferation. One objective of this invention is to provide a general synthetic route for this type of degrader and several preferred compound examples (denoted as P1~P7), and to demonstrate methods for detecting its KRAS degradation ability and antiproliferative activity in cell biology experiments (WB, cell viability assays, etc.).

The application discloses an anti-inflammatory composition based on multi-target synergistic effect, a rabdosia sasaposhiton extract and a preparation method and products and application thereof. The anti-inflammatory composition is composed of Amethystoidin A and Rabdoternin H. The rabdosia sasaposhiton extract is extracted from aboveground parts of rabdosia sasaposhiton and includes khusiyangditerpenoids without acetoxy and khusiyangditerpenoids with acetoxy. The anti-inflammatory composition and the rabdosia sasaposhiton extract can produce synergistic inhibitory effects on NF-kappa B pathways and MAPK pathways, and can be applied in the preparation of medicines, health products, functional foods or cosmetics for preventing or treating inflammatory diseases. The anti-inflammatory composition and the rabdosia sasaposhiton extract are proved to have anti-inflammatory soothing repair effects from aspects of cytology, animal model experiments and human skin test experiments, are high in efficiency and low in toxicity, and provide new candidate active substances for multi-target anti-inflammatory medicines and cosmetics.

The application discloses a kind of based on multimodal fusion's thyroid nodule BRAF gene mutation prediction system and method, belong to medical auxiliary diagnosis field, including: data acquisition module, for obtaining the conventional ultrasound image of target thyroid nodule, real-time shear wave elastography quantitative data and puncture cytology image;Multimodal feature extraction module includes ultrasound feature extraction unit, elastic feature extraction unit and cytology feature extraction unit;Feature fusion module is configured to by based on the deep learning model of attention mechanism, the ultrasound image depth feature, the elastic feature and the cytology depth feature are adaptively fused in feature level, and obtain fusion feature;Prediction module is configured to the fusion feature is input into classifier, and the prediction result that whether the target thyroid nodule exists BRAF gene mutation is output.For the precise diagnosis and treatment of thyroid nodule, individualized treatment decision and clinical process optimization provide innovative technical support.

The present invention relates of an ex vivo method for determining a reference proteomic profile of extrahepatic cholangiocarcinoma in the presence of bile duct stricture in a subject, comprising steps of : a) quantification of the protein abundances of all proteins identified in at least one bile duct cytological sample from at least one subject for which a diagnosis of extrahepatic cholangiocarcinoma has been previously established, or having benign stricture, b) quantification of the protein abundances of all proteins identified in human bile duct cells standard reference, c) determining the proteomic profile of each of said at least one sample by comparing protein abundances of each protein in the at least one sample with respect to the standard reference, d) determining a reference proteomic profile for extrahepatic cholangiocarcinoma condition, by means of a statistical test established with the proteomic profiles determined in step c). The present invention also relates to a reference proteomic profile of extrahepatic cholangiocarcinoma in the presence of bile duct stricture, and to an ex vivo method for differential diagnosis of extrahepatic cholangiocarcinoma in the presence of bile duct stricture of a subject.

The present invention reveals a portable device for staining and analyzing histopathological samples. It comprises a main base on which cylindrical structures are mounted. Each structure contains a slide container, which is stained individually. The reagent can be reused thanks to a bidirectional pump that returns it to the container after use. To stain only one sample per container, a circular base with a rectangular perforation allows the reagent to flow from a reagent container to the slide container. If another sample needs to be stained, a stepper motor rotates the cylindrical structure, positioning another slide container over the rectangular perforation. A camera captures images for analyzing the stained cytological or histological samples.The configuration of the invention's operation and the visualization of the results obtained can be done using a smart device or a screen located on the side of the main base.

Provided herein are devices, systems, and methods of using the devices and systems that enable manual and automated sampling and preparation of biological samples for evaluation. Any number of samples can be obtained, including nano / micro / microfluidic quantities. The samples include cells and / or other biological particles that are suspended or grown on a culture medium such as microcarriers, and can be obtained from one or more vessels (e.g., single well plates, vials, flasks, or bioreactors). The instruments to which the samples are transferred can include any analytical instrument, such as optical or laser force cytometry instruments.

The application relates to a method for efficiently creating a far-cross hybrid between Chinese cabbage and the genus Eruca, which comprises the following steps: taking the selfing line 'ASTRO' of the genus Eruca as a male parent, wherein the selfing line 'ASTRO' has excellent properties such as drought and flood resistance, disease resistance, salt and alkali tolerance, and the like; taking an excellent Chinese cabbage material '72049' as a female parent; spraying magnesium chloride hexahydrate; and then performing intergeneric hybridization. After embryo rescue and colchicine doubling, a doubled material is obtained, and the doubled material is transplanted into a field. Through cytological identification, morphological observation and pollen fertility identification, the doubled material is finally determined as a true hybrid plant.

Abstract A method for detecting a biological marker in a sample, preferably a biological marker on a cancer cell, the method comprising: (i) contacting the sample with at least one DNA aptamer coupled to a first reagent and wherein the aptamer specifically binds to a first biological marker in the sample to form a first complex; (ii) contacting the first complex of (i) with a second binding agent that specifically binds to the first reagent such that a second complex is formed, wherein the second binding agent is coupled to a reporter molecule; (iii) contacting the second complex (ii) with a substrate for the reporter molecule of the at least one second binding agent; and (iv) detecting the substrate reaction by formation of a reaction product thereby detecting the biological marker in the sample. Biological markers include cytokeratin 8 (CK8), cytokeratin 18 (CK18), human epidermal growth factor receptor 2 (HER2) and epithelial cell adhesion molecule (EpCAM). Abstract 41 / 50 FIGURE 40 Frozen section preparation 4 Sample fixation + Wash (10 dips in PBS) DNA aptamer staining DAB Substrate DAB substrate HRP FAM Anti-FITC conjugated with HRP FAM DNA Aptamer conjugated with FAM HRP Frozen section slide / Touch imprint slide / Cytology slide / Direct smear slide Anti-FITC-HRP Wash (10 dips in PBS) DAB Wash (10 dips in PBS) Hematoxylin counter-staining Wash under tap water Cove rs lip DAB brown precipitate at the location of the aptamer B o min 1 min 15 sec 5 min 15 sec 5 min 15 sec 5 min 15 sec 10 sec 1 min 1 min A + Wash (10 dips in PBS) Total time for aptamer-DAB staining: —18 min 4 1 / 5 0 A B 1 0 m i n 1 m i n D A B s u b s t r a t e 1 5 s e c H R P A n t i - F I T C 5 m i n D N A a p t a m e r s t a i n i n g H R P 1 5 s e c 5 m i n 1 5 s e c D N A A p t a m e r c o n j u g a t e d w i t h F A M 5 m i n D A B W a s h ( 1 0 d i p s i n P B S ) F r o z e n s e c t i o n s l i d e / T o u c h i m p r i n t s l i d e / C y t o l o g y s l i d e / D i r e c t s m e a r s l i d e c o u n t e r - s t a i n i n g 1 m i n W a s h u n d e r t a p w a t e r 1 m i n C o v e r s l i p F I G U R E 4 020 25 28 36 33 19 D ec 2 02 5 1 9 D e c 2 0 2 5 A B 1 0 m i n F r o z e n s e c t i o n p r e p a r a t i o n 1 m i n S a m p l e f i x a t i o n D A B 2 0 2 5 2 8 3 6 3 3 s u b s t r a t e 1 5 s e c W a s h ( 1 0 d i p s i n P B S ) H R P A n t i - F I T C 5 m i n D N A a p t a m e r s t a i n i n g H R P 1 5 s e c 5 m i n A n t i - F I T C - H R P T o t a l t i m e f o r 1 5 s e c D N A A p t a m e r W a s h ( 1 0 d i p s i n P B S ) c o n j u g a t e d w i t h F A M 5 m i n D A B s t a i n i n g : ~ 1 8 m i n W a s h ( 1 0 d i p s i n P B S ) F r o z e n s e c t i o n s l i d e / T o u c h i m p r i n t s l i d e / 1 0 s e c H e m a t o x y l i n C y t o l o g y s l i d e / D i r e c t s m e a r s l i d e c o u n t e r - s t a i n i n g 1 m i n W a s h u n d e r t a p w a t e r 1 m i n C o v e r s l i p F I G U R E 4 0

This invention discloses an intelligent screening method and system for abnormal cells in cervical liquid-based cytology images, belonging to the field of medical image processing technology. The method includes: acquiring and preprocessing a full-field digital image of a liquid-based thin-layer cytology smear; extracting individual cell contours using a cell instance segmentation network; calculating morphological features such as nuclear area, nucleocytoplasmic ratio, chromatin distribution, and nuclear membrane regularity; sequentially distinguishing normal cells, atypical squamous cells, low-grade and high-grade squamous intraepithelial lesions using a cascaded classification strategy; locating abnormal cells and screening high-risk fields of view based on an attention-guided algorithm; and outputting an abnormal cell annotation atlas and TBS classification assistance suggestions.

This invention discloses the E3 ubiquitin ligase ASB7 as a molecular marker for predicting PARP inhibitor therapy sensitivity and its application. Through cell biology experiments and animal model studies, this invention found that tumor cells and tissues with high ASB7 expression exhibit significant sensitivity to PARP inhibitors. Specifically, ASB7 induces homologous recombination repair (HRR) deficiency, increasing genomic instability and thus enhancing the sensitivity of tumor cells to PARP inhibitors. This ASB7 molecular marker provides new insights for precision medicine, offers an optimized treatment strategy for tumor patients with high ASB7 expression, and lays a solid foundation for the development of highly effective anti-tumor drugs, demonstrating significant application prospects and clinical significance.

The application discloses a cell image recognition method and device based on a large language model. When detecting a cervical cytology whole section image to be recognized, if positive cells are detected, the corresponding positive cells are further recognized by a preset recognition model to determine whether the corresponding positive cells are positive; and the recognition result of the cervical cytology whole section image to be recognized is determined according to the recognition result output by the preset recognition model; a preset large language model in the preset recognition model combines image features and text features to generate an explanatory text conforming to pathological logic as a recognition result; the method can improve the recognition accuracy of the cell image, and thus can improve the efficiency and reliability of early screening of cervical cancer.

The present application relates to the technical field of image detection, and discloses a cervical whole slide image detection method, a device, and a computer program product. The cervical whole slide image detection method comprises: acquiring a cervical cytology whole slide image to be detected; cropping said cervical cytology whole slide image to obtain an image block; then inputting the image block into a cell detector to obtain a preset cell image in the image block outputted by the cell detector; and performing feature extraction on the preset cell image by means of a feature extraction network in a slide classifier, and predicting an extracted feature by means of a multi-instance learning classifier in the slide classifier to obtain a cervical cytology whole slide image detection result. By executing the above operations, the accuracy of cervical whole slide image detection is improved.

The present invention provides a high throughput method for constructing and screening a compound library and a reaction device. Specifically, the method of the present invention comprises: (a) providing a reactor comprising n independent and addressable reaction chambers; (b) performing m independent synthesis reactions in said n reaction chambers, thereby constructing a compound library; and (c) performing activity tests in reaction chambers in which synthesis reactions are performed. In the present invention, the preparation and screening processes of a compound can be completed in the same reaction system. As the reactions of the present invention almost quantitatively generate products, the products can be directly used in enzymatic or even cytological activity test experiments without separation.

An apparatus for detaching cells or tissue from a subject's cavity, the apparatus comprising: an elongated member arranged to move within a lumen of a needle or catheter; and at least one flexible member arranged at a distal end of the elongated member, wherein the flexible member is configured between a first constrained configuration within the lumen of the needle or catheter and a second deployed configuration outside the lumen, wherein the flexible member in the second deployed configuration is configured to conform to the internal geometry of the cavity into which the apparatus is inserted.

The application discloses a kind of long-term treatment strategy making methods for cervical cancer prevention and treatment of HPV, belong to medical information processing technical field.The method includes: obtaining the digital HPV health record containing multiple HPV typing detection results, cervical cytology history record and individual physiological characteristic parameters;Three-dimensional treatment situation cube is constructed by virus infection state subspace, cervical tissue state subspace and treatment intervention state subspace;In the cube, state backtracking and forward-looking deduction algorithm based on time axis are used, virus clearance probability field and lesion outcome probability field are calculated;High-risk persistent infection space-time region and low-risk self-healing tendency space-time region are demarcated;Current patient state is projected to the cube, if fall into high-risk region, then trigger long-term treatment strategy generation process including multiple treatment stages, output intervention opportunity, intensity and state transition condition of each stage.

The present invention relates to a computer-implemented method for detecting one or more defined genomic aberrations from images of a body fluid, bone marrow or cytological sample, such as a cytological smear sample, in particular a blood smear sample.

This invention provides a method for efficient isolation of callus protoplasts from Brazilian bananas and its application, belonging to the field of plant cell biology. The method for isolating Brazilian banana protoplasts involves: cutting unexpanded heart leaves of Brazilian bananas into strips and mixing them with an enzymatic hydrolysate at a ratio of 4-5 g: 15 mL for enzymatic hydrolysis. The enzymatic hydrolysate comprises the following components at the following concentrations: cellulase 1-1.5 w / v%, pectinase 0.2-0.4 w / v%, mannitol 0.35-0.45 M, KCl 18-22 mM, MES 18-22 mM, and CaCl₂. 2 8~12 mM, BSA bovine serum albumin 0.08~0.12%. This invention, by improving the enzymatic hydrolysate formulation, significantly enhanced the viability of protoplasts from the Brazilian banana plantation, achieving a protoplast yield of 3.1 × 10⁻⁶. 6 g ‑1 The viability of the protoplasts prepared by this invention is as high as 92%. These protoplasts can be used for subcellular localization and bimolecular fluorescence complementation experiments, laying a solid foundation for gene function research and molecular breeding work in the Musa genus, and also demonstrating good potential feasibility for their application in plant regeneration.

The present application relates to the technical field of rapid immunohistochemistry, and particularly relates to a rapid immunohistochemical staining method for pleural effusion cytology. The method comprises the following steps: S1, taking the pleural effusion for processing to obtain cell sediment, drying the water of the cell group, and embedding; S2, placing the cell group on a freezing mold, wrapping with OCT embedding agent, and placing in a quick freezer to quickly freeze into a block, and then slicing; S3, taking several slices from the prepared cell group freezing block of S2, and using flowing water and PBS to clean the OCT embedding agent on the slices, and then placing the slices in a preheated citric acid buffer for medium-low fire repair; S4, after the slice repair is completed, using an OptiView DAB IHC Detection Kit to perform manual immunohistochemical staining on the slice. The rapid immunohistochemical staining technology for pleural effusion cytology established by the present application can obtain consistent diagnostic results with conventional paraffin methods within 2 hours, and provides reliable support for intraoperative rapid cytological pathological diagnosis.

This invention discloses a fully automated liquid-based cytology slide preparation and staining machine, comprising a frame, a turntable assembly, a lateral movement assembly, a lifting assembly, a push rod assembly, a solenoid valve assembly, a waste collection assembly, and a waste liquid collection assembly. The turntable assembly integrates sample bottles, pipette tips, slide clamps, and sedimentation baskets, enabling multi-channel sample loading. The lateral movement assembly and the lifting assembly work together to perform pipetting, sample addition, filter placement and removal, and staining operations. The push rod assembly drives the turntable assembly to perform centrifugal slide preparation. The solenoid valve assembly controls the actions of each actuator. The waste collection assembly and waste liquid collection assembly respectively achieve centralized treatment of waste and waste liquid. This invention integrates centrifugal slide preparation and automatic staining functions. The equipment has a compact layout and small footprint. It uses standard consumables and operates fully automatically. The multi-channel design improves slide preparation and staining efficiency, solving the technical problems of high cost and limited functionality of existing TCT equipment and cumbersome processes and high operational requirements of LCT equipment. It is suitable for liquid-based cytology testing scenarios in pathology departments.

The present application relates to the field of crop breeding technology, and specifically discloses a wheat tandem translocation chromosome, a molecular marker thereof and application, wherein the translocation chromosome comprises a segment of 637-812 Mb at the end of the long arm of a wheat 2B chromosome replaced by the long arm of a rye 2R chromosome and the 2S chromosome of a variable goat grass v to form a 2BS.2BL-2RL-2S v L tandem translocation chromosome. The present application creates a tandem translocation chromosome containing a powdery mildew resistance gene Pm2R QL and a stripe rust resistance gene YrAev , and develops a supporting molecular marker, solves the problem that after endogenous disease resistance genes are aggregated in wheat breeding, the identification of exogenous chromosome fragments relies on time-consuming and laborious cytological methods and is difficult to be applied in high-throughput, and quickly and accurately distinguishes between 2BS.2BL-2RL-2S v L tandem translocation chromosome carriers and non-carriers, replaces traditional cytological identification, supports molecular marker-assisted breeding, and accelerates the breeding process of new varieties of multi-resistant wheat.

This application provides a method, system, device, medium, and program product for analyzing bone marrow metastases in neuroblastoma, relating to the fields of intelligent medical image analysis and artificial intelligence-assisted diagnosis. The application includes: obtaining digitized whole-slice images of bone marrow cells from bone marrow cytological smears and preprocessing these images; preprocessing the whole-slice images to obtain bone marrow cell image blocks; weighting the bone marrow cell image blocks to obtain bone marrow cell feature images; wherein, the weighting processing includes feature extraction from the bone marrow cell image blocks to obtain corresponding feature vectors, employing multi-instance learning to represent the feature vectors at the whole-slice level to obtain bone marrow cell feature images; and classifying the bone marrow cell feature images to obtain corresponding classification results. This application achieves effective fusion from local cytological features to whole-slice-level diagnostic results through a multi-instance learning aggregation module.