6 results about "HPV Positive" patented technology

The present application relates to the technical field of medical data processing, and particularly relates to a cervical disease two-stage dynamic risk prediction method based on multidimensional data, comprising the following steps: step S1, data acquisition and structuring: a plurality of dimensions of patient data are collected and structured by questionnaire, and a cervical disease database is constructed; step S2, first-stage model construction: for a general screening population, a first prediction model is constructed by using the questionnaire survey data collected in step S1; step S3, second-stage model construction: for a population with HPV positive or other suspicious indications obtained by the first prediction model, high-dimensional clinical data collected in step S1 are integrated, and a second prediction model is constructed by using an ensemble learning strategy; step S4, model series early warning output; and step S5, decision support generation. The present application integrates low-cost and easily-obtained multidimensional data, constructs a scientific risk stratification model, and realizes two-stage and continuous dynamic early warning.

The invention discloses an accurate early warning auxiliary diagnosis model system based on high-risk HPV positive, and relates to the technical field of medical diagnosis, according to the accurate early warning auxiliary diagnosis model system based on high-risk HPV positive, by obtaining the relevance and action intensity of conventional blood and urine test indexes and high-risk human papilloma virus (HR-HPV) infection, the accurate early warning auxiliary diagnosis model system based on high-risk HPV positive can be used for early warning of high-risk human papilloma virus (HR-HPV) infection. A basis is provided for deep research in the field; an HR-HPV infection positive early warning model taking health screening big data as a basis and hematuria biological indexes as elements is formed, and the problems that HPV detection depends on subjective judgment of doctors or active selection of clients, an accurate measurement basis is lacked, and the screening coverage rate is not enough are solved. Therefore, a simple, convenient, rapid and economical means is provided for gynecological evaluation, reliable indications are provided for HPV detection, the screening efficiency is improved, and help is provided for prevention of HPV infection and cervical diseases.

The invention discloses a screening method of action targets of traditional Chinese medicine phellinus linteus and application of the screening method to treatment of HPV positive tumors, and the screening method comprises the following steps: (1) screening potential action targets of phellinus linteus, respectively obtaining pathogenic targets of HPV infected cervical cancer by combining a GeneCards database, an OMIM database and a DisGeNET database, and taking intersection to obtain common targets; (2) obtaining a protein-protein interaction network of the intersection targets by using an STRING database, and constructing a PPI network between the intersection targets; (3) carrying out GO function enrichment and KEGG pathway enrichment analysis on the core target spot; and (4) carrying out molecular docking on the identified potential target spot of the phellinus linteus. The traditional Chinese medicine phellinus linteus ketone screened by the method disclosed by the invention can accurately target HPV virus, promote degradation of key oncoprotein E6 / E7 and play an anti-tumor role by regulating mitochondrial autophagy and reducing growth and metastasis of HPV-induced cervical cancer, and the medicine is identified to be low in cost and small in toxic and side effects and has relatively good treatment potential in HPV positive tumors.

Methods and compositions are provided for the detection of HPV infection in a subject sample. In particular, the methods and composition include variants of certain HPV proteins (e.g., HPV16 E6 protein), wherein the HPV proteins have been modified to enhance solubility while maintaining or enhancing seropositivity to HPV positive plasma / sera. Additional methods and compositions relate to improving purification of HPV proteins through the use of modified amino acid sequences. These approaches may enhance the performance of serological detection techniques and support broader implementation of detection assays.

Provided are a method of using a circular RNA as vaccine to treat HPV-positive cancer and a design strategy to elicit specific and robust T cell response by promoting antigen presentation.

Disclosed in the present application are the use of an antigen short peptide in screening a drug for treating HPV-related diseases and a TCR screened by same, wherein the amino acid sequence of the antigen short peptide is as shown in SEQ ID NO: 1 or SEQ ID NO: 2. In the present application, the antigen short peptide can be used to screen a specific T cell receptor (TCR), and the T cell transduced with the TCR can be specifically activated and have a very strong killing effect on tumor cells expressing A1101 and HPV, which can be used for immunotherapy of HPV positive tumors, such as cervical cancer. In addition, the T cell transduced with the TCR of the present application has a strong activation reaction on a cell line expressing E6, has no activation reaction on a cell line not expressing E6, and has a very strong killing function on the cell line expressing E6 and can effectively inhibit the growth of E6 positive tumors.