3 results about "HPV Positive" patented technology

The present application relates to the technical field of medical data processing, and particularly relates to a cervical disease two-stage dynamic risk prediction method based on multidimensional data, comprising the following steps: step S1, data acquisition and structuring: a plurality of dimensions of patient data are collected and structured by questionnaire, and a cervical disease database is constructed; step S2, first-stage model construction: for a general screening population, a first prediction model is constructed by using the questionnaire survey data collected in step S1; step S3, second-stage model construction: for a population with HPV positive or other suspicious indications obtained by the first prediction model, high-dimensional clinical data collected in step S1 are integrated, and a second prediction model is constructed by using an ensemble learning strategy; step S4, model series early warning output; and step S5, decision support generation. The present application integrates low-cost and easily-obtained multidimensional data, constructs a scientific risk stratification model, and realizes two-stage and continuous dynamic early warning.

The invention discloses a screening method of action targets of traditional Chinese medicine phellinus linteus and application of the screening method to treatment of HPV positive tumors, and the screening method comprises the following steps: (1) screening potential action targets of phellinus linteus, respectively obtaining pathogenic targets of HPV infected cervical cancer by combining a GeneCards database, an OMIM database and a DisGeNET database, and taking intersection to obtain common targets; (2) obtaining a protein-protein interaction network of the intersection targets by using an STRING database, and constructing a PPI network between the intersection targets; (3) carrying out GO function enrichment and KEGG pathway enrichment analysis on the core target spot; and (4) carrying out molecular docking on the identified potential target spot of the phellinus linteus. The traditional Chinese medicine phellinus linteus ketone screened by the method disclosed by the invention can accurately target HPV virus, promote degradation of key oncoprotein E6 / E7 and play an anti-tumor role by regulating mitochondrial autophagy and reducing growth and metastasis of HPV-induced cervical cancer, and the medicine is identified to be low in cost and small in toxic and side effects and has relatively good treatment potential in HPV positive tumors.

Methods and compositions are provided for the detection of HPV infection in a subject sample. In particular, the methods and composition include variants of certain HPV proteins (e.g., HPV16 E6 protein), wherein the HPV proteins have been modified to enhance solubility while maintaining or enhancing seropositivity to HPV positive plasma / sera. Additional methods and compositions relate to improving purification of HPV proteins through the use of modified amino acid sequences. These approaches may enhance the performance of serological detection techniques and support broader implementation of detection assays.