The invention discloses the technical field of gynecologic products and particularly relates to the field of HPV positive-to-negative spray. The HPV positive-to-negative spray is prepared from the following components in parts by weight: 30 to 40 parts of a blueberry leaf extract, 27 to 37 parts of herba patriniae, 25 to 35 parts of triethanol, 25 to 35 parts of chrysanthemum indicum, 25 to 35 parts of callicarpa nudiflora, 18 to 28 parts of radix lithospermi, 18 to 28 parts of polygonum tinctorium, 15 to 25 parts of curcuma zedoary, 15 to 25 parts of rhizoma coptidis, 14 to 24 parts of flos lonicerae, 8 to 18 parts of fructus cnidii, 5 to 15 parts of herba houttuyniae and the balance of normal saline. The HPV positive-to-negative spray disclosed by the invention selects traditional Chinese medicines as raw materials and has the advantages of easiness in raw material obtaining, reasonable formula, small toxic and/or side effects and high rate of HPV positive-to-negative; by using the HPV positive-to-negative spray, the efficacies of clearing away heat and toxic materials, and resisting bacteria, inflammation and viruses are realized, an HPV virus is effectively inhibited and HPV positive-to-negative conversion is realized.