30 results about "Cervical diseases" patented technology

The invention relates to the technical field of biological detection, and discloses a reagent composition and a kit for detecting cervical cancer and precancerous lesions of cervical cancer and application of the reagent composition and the kit. The reagent composition provided by the invention is used for detecting the methylation degree of a specific marker gene, and the detection sensitivity and specificity of cervical cancer and high-grade cervical cancer precancerous lesions are at a relatively high level. When the reagent composition is used for screening cervical cancer and precancerous lesions of HPV positive people, compared with conventional cytological detection, the colposcope referral rate is greatly reduced, meanwhile, the detection times for definite diagnosis of high-grade cervical lesions or cervical cancer are reduced, and the waste of medical resources and the examination burden of patients are reduced.

The invention provides a biomarker for cervical lesion caused by low folic acid mediated HPV infection and application of the biomarker, the biomarker is IGF2BP3 and miR-27a, and the IGF2BP3 and the miR-27a can be used as a therapeutic target and a research tool for cervical lesion caused by low folic acid mediated HPV infection. The small nucleic acid targeting IGF2BP3 and miR-27a is delivered through the folic acid modified exosome in cervical lesion treatment, and the delivery efficiency and the treatment effect are remarkably improved.

The invention relates to the technical field of external traditional Chinese medicine plasters, in particular to a yang-activating, cold-removing and pain-relieving ointment and a preparation method thereof. The analgesic ointment is prepared by taking black sesame oil and yellow lead as matrixes and assisting coarse medicinal materials with the effects of warming yang, dispelling cold, promoting blood circulation and dredging collaterals and fine medicinal materials (musk, camphor, myrrh, frankincense and the like) through the steps of treating the medicinal materials, extracting, refining oil, adding the yellow lead to form the ointment, removing fire toxin, adding the fine materials, spreading and the like. The traditional Chinese medicine composition does not contain western medicine components, can warm yang and dredge vessels, dispel cold and wind and eliminate dampness, promote blood circulation to remove meridian obstruction and remove blood stasis, and relieve swelling and pain, and is suitable for treating various orthopedic and rheumatism diseases such as acute and chronic lumbar and cervical diseases, knee joint hyperosteogeny, sciatica and scapulohumeral periarthritis. The ointment is large in drug loading capacity, capable of being administrated for a long time, good in long-term curative effect, not prone to relapse after healing, capable of being directly applied without heating, convenient to use, free of deterioration in two years at the normal temperature, small in irritation, low in anaphylaxis and high in safety.

The utility model relates to the technical field of research, development and design of pet supplies, in particular to a novel pet bowl which comprises a supporting seat and a containing part, a food containing space used for containing pet food is arranged in the containing part, the containing part is provided with a rotating shaft part, the supporting seat is provided with a through hole corresponding to the rotating shaft part, and the supporting seat is provided with a through hole corresponding to the rotating shaft part. The pet feeding device has the advantages that the angle of the containing piece can be adjusted by adjusting the matching of the rotating shaft part and the through hole, the degree that a pet needs to lower the head during feeding is effectively reduced, and the pet feeding device is simple in structure, convenient to use and high in practicability. A user can adjust the containing part at any time according to the actual condition of a pet to reach the angle suitable for the pet, it is ensured that the pet keeps a natural and comfortable posture during feeding, therefore, pressure on the neck of the pet is relieved, cervical vertebra diseases can be prevented, the reasonable feeding angle is beneficial to more smooth feeding of the pet, and the feeding efficiency is improved.

The invention patent is titled "Dragon and Cypress Gel," belonging to the pharmaceutical category. The main components of Dragon and Cypress Gel are: Phellodendron bark, earthworm, dragon's blood, Oldenlandia diffusa, Sophora flavescens, and Bletilla striata. In recent years, research on cervicitis and cervical lesions has shown good results using external treatments of traditional Chinese medicine. Vaginal cervical medications are characterized by high local drug concentration, low dosage, and safety and effectiveness, receiving high clinical evaluation. The vagina and cervix have abundant capillaries and lymphatic vessels, and lack clearly defined nerve endings, resulting in minimal pain stimulation for patients during administration. This makes it an effective drug release site for specific diseases such as cervicitis and vaginitis. Traditional Chinese medicine gels are a novel type of external preparation of traditional Chinese medicine. They are made by dissolving or uniformly dispersing traditional Chinese medicine in a gel. These gels adhere tightly to the treatment site for a relatively long time, exhibiting good bioadhesion, and are simple to prepare. It is suitable for use on skin, mucous membranes and cavities. It not only avoids the first-pass effect of enzymes and acids in the gastrointestinal tract that exists in oral administration, but also reduces the adverse reactions of drugs. It has the characteristics of good spreadability, non-greasy feeling, easy to wash off, good transdermal absorption, and comfortable use.

The invention discloses a nano vaccine for treating HPV (human papilloma virus) infection related cervical lesions as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. In order to solve the problem that the response rate of an existing cervical lesion treatment vaccine does not meet clinical requirements, the hybrid membrane with enhanced immunogenicity and improved safety is prepared by applying a membrane fusion technology and combining an autologous tumor cell membrane and an escherichia coli cytoplasmic membrane; meanwhile, PLGA nano particles loaded with HPV16 E5 / E6 / E7 specific CTL epitope long peptide are used as a delivery carrier, and the HM-NPs-LP nano vaccine co-delivering the hybrid membrane and the HPV16 specific antigen peptide is developed. According to the nano vaccine, tumor cells and HPV16 E5 / E6 / E7 specific CTL epitope long peptide are taken as antigens for immunotherapy, dual immune activation of broad spectrum and specific antigens can be realized, personalized multi-effect treatment capacity for HPV infection related cervical diseases is exerted, targeted killing of HPV infection and pathological cells is enhanced, pathological relapse is prevented, and the nano vaccine has good clinical application prospects. And a new strategy is provided for individualized precise immunotherapy.

According to the cervical cell automatic detection method based on the improved YOLOv8 model, the method is improved based on the YOLOv8 model, the framework of the YOLOv8 model is emphatically optimized, and the recognition precision is improved. A large separable kernel attention (LSKA) module is introduced into the improved YOLOv8 model, and a large Separable KernelAttention (LSKA) module is introduced into the improved YOLOv8 model. The module can more effectively capture the multi-scale features of cervical cells through large-kernel separation convolution and an attention mechanism, improves the recognition capability of cell details, and achieves more comprehensive and accurate target positioning and recognition. The method provided by the invention effectively improves the recognition accuracy of cervical cells, provides an efficient and reliable auxiliary means for early diagnosis and analysis of cervical diseases, has a wide application prospect, and is expected to play an important role in the field of medical diagnosis.

1. The name of the design product: cervical treatment tablets. 2. The use of the design product: medicinal materials for treating cervical diseases. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view 1.

The present invention discloses a method for establishing a cervical disease progression prediction model based on low-depth whole-genome sequencing technology, which uses cervical scraping or vaginal swabs to collect exfoliated cells, extract DNA, and perform whole-genome sequencing to obtain the original offline data of low-depth whole-genome sequencing of the DNA sample; the above-mentioned original data is subjected to standard quality control, and then compared with the human reference genome sequence and the repetitive sequences are marked; the genome instability index is calculated by the following formula:; In addition, the present invention also relates to a method for constructing a cervical disease prediction model; the present invention is conducive to improving the accuracy and sensitivity of detection, reducing the cost of detection, and improving the accessibility of cervical disease screening and prediction, while contributing to the early diagnosis of cervical disease and the formulation of treatment decisions, and improving the management and treatment effects of patients with cervical disease.

The present application relates to the technical field of medical data processing, and particularly relates to a cervical disease two-stage dynamic risk prediction method based on multidimensional data, comprising the following steps: step S1, data acquisition and structuring: a plurality of dimensions of patient data are collected and structured by questionnaire, and a cervical disease database is constructed; step S2, first-stage model construction: for a general screening population, a first prediction model is constructed by using the questionnaire survey data collected in step S1; step S3, second-stage model construction: for a population with HPV positive or other suspicious indications obtained by the first prediction model, high-dimensional clinical data collected in step S1 are integrated, and a second prediction model is constructed by using an ensemble learning strategy; step S4, model series early warning output; and step S5, decision support generation. The present application integrates low-cost and easily-obtained multidimensional data, constructs a scientific risk stratification model, and realizes two-stage and continuous dynamic early warning.

The invention discloses an accurate early warning auxiliary diagnosis model system based on high-risk HPV positive, and relates to the technical field of medical diagnosis, according to the accurate early warning auxiliary diagnosis model system based on high-risk HPV positive, by obtaining the relevance and action intensity of conventional blood and urine test indexes and high-risk human papilloma virus (HR-HPV) infection, the accurate early warning auxiliary diagnosis model system based on high-risk HPV positive can be used for early warning of high-risk human papilloma virus (HR-HPV) infection. A basis is provided for deep research in the field; an HR-HPV infection positive early warning model taking health screening big data as a basis and hematuria biological indexes as elements is formed, and the problems that HPV detection depends on subjective judgment of doctors or active selection of clients, an accurate measurement basis is lacked, and the screening coverage rate is not enough are solved. Therefore, a simple, convenient, rapid and economical means is provided for gynecological evaluation, reliable indications are provided for HPV detection, the screening efficiency is improved, and help is provided for prevention of HPV infection and cervical diseases.

The invention relates to the technical field of biological detection, and discloses a reagent composition for cervical cancer detection, a kit and application. The reagent composition comprises a reagent for detecting the methylation level function of cervical cancer marker genes or fragments thereof, and the marker genes comprise a PAUPAR gene, an NETO1 gene and an NTM gene. According to the reagent composition, the methylation level of a specific marker gene combination is detected, efficient non-invasive screening of high-grade cervical lesions and cervical cancer can be achieved, and high detection specificity and sensitivity are achieved.

Devices, systems and methods are provided for treating conditions of the reproductive tract. A number of conditions can afflict the lining and cell layers deeper within the anatomical structures. For example, cervical intraepithelial neoplasia (CIN), also known as cervical dysplasia, is a condition involving abnormal growth of cells on the surface of the cervix that could potentially lead to cervical cancer in situ (CIS). Other conditions include human papillomavirus (HPV)-related cervical disease, various endometrial diseases, acute and chronic cervicitis, and various infections (e.g. trichomoniasis) to name a few. In some embodiments, treatments eliminate diseased, damaged, abnormal or otherwise undesired cells leaving the tissue framework intact. This allows the tissue to regenerate in a normal fashion, avoiding the formation of scar tissue. When the tissue framework is left intact, the framework structure repopulates with healthy cells, regenerating the normal tissue without altering the structural properties.

Devices, systems and methods are provided for treating conditions of the reproductive tract. A number of conditions can afflict the lining and cell layers deeper within the anatomical structures. For example, cervical intraepithelial neoplasia (CIN), also known as cervical dysplasia, is a condition involving abnormal growth of cells on the surface of the cervix that could potentially lead to cervical cancer in situ (CIS). Other conditions include human papillomavirus (HPV)-related cervical disease, various endometrial diseases, acute and chronic cervicitis, and various infections (e.g. trichomoniasis) to name a few. In some embodiments, treatments eliminate diseased, damaged, abnormal or otherwise undesired cells leaving the tissue framework intact. This allows the tissue to regenerate in a normal fashion, avoiding the formation of scar tissue. When the tissue framework is left intact, the framework structure repopulates with healthy cells, regenerating the normal tissue without altering the structural properties.

The invention relates to the technical field of image recognition model algorithms, in particular to a construction method of a colposcope intelligent auxiliary diagnosis decision-making system, which comprises the following steps of: S1, widely collecting colposcope image data covering different lesion degrees on the basis of a multi-modal cervical special disease database, marking the image data one by one by a qualified doctor, accurately delimiting a high-risk area of high-level cervical lesions to form a target detection annotation data set; s2, performing data preprocessing on the data obtained in the step S1, including performing systematic cleaning and enhancement processing on an original image, and performing focus area detection processing on the preprocessed data; and S3, inputting the candidate region detected in the step S2 into a feature learning network. According to the method, spatial distribution characteristics of lesion severity are fully considered, and an area with the most likely existence of HSIL is preferentially recommended and covered, so that biopsy targeting and diagnosis efficiency are improved.

The invention belongs to the technical field of medical animal model construction, and particularly relates to a construction method of an animal model for simulating various stages from HPV (human papillomavirus) continuous infection to cervical canceration in a natural infection state and application of the animal model. According to the method, a drug immunosuppressor for promoting HPV infection is combined with MmuPV1 for mouse lower genital tract infection modeling; the constructed animal model is stable and uniform in papilloma virus infection, high in modeling rate, low in fatality rate, short in modeling time, controllable in process and good in reproducibility, can completely reproduce the whole process from HPV continuous infection to cervical canceration, accords with clinical practice, improves the efficiency and quality of model construction, and is suitable for popularization and application. An ideal animal model is provided for follow-up research on an HPV infection carcinogenic mechanism and a virus host interaction mechanism, meanwhile, the model can assist in research, development and screening of clinical treatment methods for preventing and treating cervical lesions at all stages, and clinical transformation, new drug research and development and other work are promoted.

The present application relates to the technical field of image recognition model algorithm, in particular to a kind of colposcope intelligent auxiliary diagnosis decision system construction method, step S1, with multi-modal cervical disease database as the basis, the image data of different lesion degrees is widely collected, the image data is annotated by experienced doctor, and the high-risk area of high-grade cervical lesion is accurately demarcated to form target detection annotation dataset;Step S2, data preprocessing is carried out to the data obtained in step S1, including systematic cleaning and enhancement processing to the original image, and lesion area detection processing is carried out to the preprocessed data;Step S3, the candidate area detected in step S2 is input into feature learning network.The method of the present application fully considers the spatial distribution characteristics of lesion severity, preferentially recommends the area most likely to exist HSIL, thereby improving the targeting and diagnostic efficiency of biopsy.

The invention discloses a group of gene methylation primer probe compositions for auxiliary identification of cervical lesions, a kit, application and a diagnosis model, and relates to the technical field of biological detection, and the primer probe compositions are related to six genes of PAX1, SOX1, EPB41L3, ST6GALNAC5, SOX14 and ZNF671. The kit comprises a nucleic acid extraction or purification reagent, a methylation detection sample pretreatment reagent, a PCR reaction mixed solution, a primer probe mixed solution 1, a primer probe mixed solution 2 and a positive and negative quality control. The composition and the kit are used for detecting HPV negative or HPV positive cervical high-grade intraepithelial neoplasia, cervical squamous carcinoma and cervical adenocarcinoma. According to the invention, the detection sensitivity and specificity can be improved, the sample input amount is reduced, and multiple detections of one tube of sample in cervical cancer screening are realized; and different histopathological types of cervical cancer can be detected.

The invention relates to the technical field of cervical lesions, and discloses a cervical lesion risk prediction model based on combination of double gene methylation and clinical characteristics and application thereof, and the method comprises the following steps: S1, selecting a research object; s2, carrying out double-gene methylation detection; and S3, data statistical analysis: performing statistical analysis by applying SPSS 25.0 and R4.1. 2 software, performing chi-square test and single-factor logistic regression analysis on each potential influence factor, and bringing the screened influence factors into multi-factor logistic regression analysis. According to the cervical lesion risk prediction model based on combination of double-gene methylation and clinical characteristics and application and development thereof, evidence-based basis can be better provided, clinical reduction of missed diagnosis and misdiagnosis rates of cervical cancer is helped, morbidity and mortality are reduced, and implementation of a strategic target of eliminating cervical cancer in China is assisted.

The invention belongs to the technical field of traditional Chinese medicines, and provides a pharmaceutical composition for cervical and vaginal lesions as well as a preparation method and application of the pharmaceutical composition. The pharmaceutical composition is prepared from the following components in parts by weight: 0.01 to 1 part of astilbin, 7.5 to 30 parts of radix sophorae flavescentis, 10 to 35 parts of herba houttuyniae, 7.5 to 30 parts of honeycomb, 6 to 24 parts of dysosma versipellis, 8 to 24 parts of fructus cnidii and 2.5 to 5 parts of oleic acid. The pharmaceutical composition disclosed by the invention has a prominent treatment effect on vagina or cervical lesions, especially vaginitis and vagina and cervical squamous intraepithelial neoplasia caused by HPV (Human Papilloma Virus).

The invention provides a cervical immunohistochemical lesion grading method, a cervical immunohistochemical lesion grading system and cervical immunohistochemical lesion grading equipment based on deep learning, and relates to the crossing field of deep learning and medicine. The method comprises the following steps: acquiring a digital image of a cervical immunohistochemical section through data acquisition and preprocessing, acquiring an RGB matrix and corresponding image data, acquiring corresponding data features through deep learning, and fusing through a Transform model; constructing a reference feature library, and presetting error thresholds of different CIN levels; and calculating a total error between the input sample and the corresponding reference feature in each sub-region, determining a lesion level of each sub-region, and performing evaluation to obtain a grading result of the current region to be graded. By introducing various modal information such as RGB image features and image space features and combining a dynamic attention mechanism and a multi-scale error evaluation strategy, automatic and refined grading of cervical lesions is realized.

The invention discloses an HPVE6 / E7-caused cervical cancer lesion early diagnosis system and application, and relates to the technical field of medical treatment, the system comprises an HPVE6 / E7 protein detection module used for quantitatively detecting the HPVE6 / E7 protein level in cervical exfoliated cells of a target object to obtain a detection result of the HPVE6 / E7 protein level; the telomerase activity detection module is used for detecting the activity of single-cell telomerase in the cervical exfoliated cells to obtain a detection result of the activity of the single-cell telomerase; the diagnosis threshold determination module is used for determining a diagnosis threshold of the HPVE6 / E7 protein level and a diagnosis threshold of the single cell telomerase activity based on the first correlation analysis result, the second correlation analysis result and the third correlation analysis result; the combined diagnosis module is used for carrying out early diagnosis on the precancerous lesion of the cervical cancer on the target object; and the risk assessment module is used for assessing the progress risk of the cervical lesion of the target object and the possibility of relapse after treatment according to the early diagnosis result of the cervical precancerous lesion.

The invention relates to the technical field of gynecological medical instruments, in particular to a gynecological cervical disease examination sampling device which comprises a first shell and a sampling assembly, an opening is formed in the first end of the first shell, the sampling assembly is arranged at the second end of the first shell, the sampling assembly comprises a driving rod, and the first end of the driving rod is fixedly connected with a sampling brush head; and the second end part of the driving rod is fixedly connected with the position adjusting assembly. The position adjusting assembly comprises a bearing disc and a rotary position adjusting rod, the driving rod is connected with the center of the side wall of the bearing disc, the rotary position adjusting rod is arranged on the edge of the side wall of the side, away from the driving rod, of the bearing disc, and a rotationally-connected sleeve is arranged at the end, away from the bearing disc, of the rotary position adjusting rod. When the sampling device is used, after an operator holds the sleeve, the driving rod and the sampling brush head can be driven to continuously rotate by rotating the position adjusting rod and the bearing disc, and the hand of the operator can do circular motion around the driving rod, so that the sleeve does not need to be loosened, the physical strength of the operator is saved, and the working efficiency of the sampling device is improved.

The utility model provides a cervix administration device which comprises a delivery part and an expansion part, the delivery part is of a cone structure, and the conical surface of the cone structure is an inwards-sunken curved surface and is matched with the shape of a cervix opening; the microneedle patch is mounted on the surface of the delivery part; the bottom of the cone structure is provided with a supporting chassis with a certain thickness, and the supporting chassis plays a supporting role on the whole; a through hole is formed in the delivery part in a penetrating manner, and the delivery part is integrally mounted on the connecting long tube of the expansion part through the through hole to form a whole, so that the micro-needle patch is delivered to the diseased region of the cervical orifice of a patient to realize micro-needle drug delivery; the dilation component is further provided with a balloon, and the purpose of slowly and controllably dilating the cervical orifice is achieved after liquid / gas is injected; therefore, the transvaginal drug delivery device specially used for delivering the microneedle patch to the cervix uteri is constructed, a delivery scheme is provided for the microneedle patch in treatment application of cervical diseases, and the treatment comfort of a patient and the treatment efficiency of medical staff are improved.

The application discloses application of VAMP8 in preparation of products for diagnosing and treating HPV16 virus infection related cervical diseases. Through research on expression and functions of VAMP8 in HPV16 infection and cervical disease progression, the application provides a new potential marker and treatment target for prevention and treatment of HPV16 related cervical diseases, and has important clinical application value. Compared with the prior art, the application can more accurately treat HPV16 infection and induced cervical diseases, reduce side effects, and improve treatment effect.

The present application relates to the technical field of biological detection, and discloses a reagent composition, a kit and application for detecting cervical cancer and precancerous lesions. MDGA2 gene, ZNF773 gene, CSDAP1 gene and CLEC14A gene. The reagent composition of the present application can realize efficient non-invasive screening of high-grade cervical lesions and cervical cancer by detecting the methylation level of a specific combination of marker genes, and has high detection specificity and sensitivity.

The present application belongs to the field of biological medicine, and particularly relates to the use of recombinant humanized collagen type XVII in the treatment of cervical cancer, and more particularly to the use of recombinant humanized collagen type XVII in the preparation of a drug for preventing and / or treating cervical diseases; the recombinant humanized collagen type XVII comprises or consists of a sequence represented by (A)m, wherein each A is selected from the amino acid sequence represented by any one of SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3; m is an integer between 1 and 10; each A is the same or different, and two adjacent As are directly connected by a peptide bond or connected by one or more amino acid residues. Research data shows that the recombinant humanized collagen type XVII can significantly inhibit the proliferation, migration, invasion and tumorigenicity of cervical cancer cells, and the inhibitory effect is dose-dependent.

The application discloses application of VAMP8 in preparation of products for diagnosing and treating HPV16 virus infection related cervical diseases. Through research on expression and functions of VAMP8 in HPV16 infection and cervical disease progression, the application provides a new potential marker and treatment target for prevention and treatment of HPV16 related cervical diseases, and has important clinical application value. Compared with the prior art, the application can more accurately treat HPV16 infection and induced cervical diseases, reduce side effects, and improve treatment effect.

The invention discloses an endometrial stem cell preparation for cervical hypertrophy and chaff scale repair and application, and belongs to the technical field of stem cells, the endometrial stem cell preparation comprises a stem cell gel carrier, endometrial stem cells, auxiliary repair polypeptide and a survival rate enhancer, in the endometrial stem cell preparation, the stem cell gel carrier is synergistically matched with the endometrial stem cells, the auxiliary repair polypeptide and the survival rate enhancer, so that the survival rate of the endometrial stem cells in vivo can be increased, and the auxiliary repair polypeptide can activate the MAPK / ERK pathway of the endometrial stem cells, so that the endometrial stem cells can be effectively repaired. The anti-inflammatory mimic peptide in the survival rate enhancer can inhibit stress silencing of the endometrial stem cells, so that the secretion function of the endometrial stem cells is ensured, and the targeted repair capacity of the endometrial stem cells is enhanced through the three-dimensional porous structure of the stem cell gel carrier; homing and colonization of the endometrial stem cells on the cervical diseased region are enhanced.

The invention belongs to the technical field of biological medicines, and discloses MMP12 as a biological marker for cervical cancer and precancerous lesions of cervical cancer and application of the MMP12 in order to solve the problem that a TIME change mechanism is still unclear in the progress process of cervical cancer at present. According to the application disclosed by the invention, the increase of MMP12 positive macrophages along with the progress of the cervical disease is determined for the first time, the MMP12 protein secreted by the MMP12 positive macrophages is a specific molecular characteristic of the progress of the cervical disease, and a new basis is provided for accurate diagnosis and treatment. Through multi-omics analysis and in-vivo and in-vitro functional experiments, it is proved that the MMP12 protein can drive growth, invasion and migration of cervical cancer tumors and shape an immunosuppression microenvironment, and the MMP12 protein is a treatment target with great potential. The effect of the MMP12 inhibitor is verified in an in-vivo and in-vitro model for the first time, and an experimental basis is provided for a treatment strategy of cervical cancer and precancerous lesions of cervical cancer.