19 results about "Cervical diseases" patented technology

The invention relates to the technical field of biological detection, and discloses a reagent composition and a kit for detecting cervical cancer and precancerous lesions of cervical cancer and application of the reagent composition and the kit. The reagent composition provided by the invention is used for detecting the methylation degree of a specific marker gene, and the detection sensitivity and specificity of cervical cancer and high-grade cervical cancer precancerous lesions are at a relatively high level. When the reagent composition is used for screening cervical cancer and precancerous lesions of HPV positive people, compared with conventional cytological detection, the colposcope referral rate is greatly reduced, meanwhile, the detection times for definite diagnosis of high-grade cervical lesions or cervical cancer are reduced, and the waste of medical resources and the examination burden of patients are reduced.

The invention provides a biomarker for cervical lesion caused by low folic acid mediated HPV infection and application of the biomarker, the biomarker is IGF2BP3 and miR-27a, and the IGF2BP3 and the miR-27a can be used as a therapeutic target and a research tool for cervical lesion caused by low folic acid mediated HPV infection. The small nucleic acid targeting IGF2BP3 and miR-27a is delivered through the folic acid modified exosome in cervical lesion treatment, and the delivery efficiency and the treatment effect are remarkably improved.

The invention relates to the technical field of external traditional Chinese medicine plasters, in particular to a yang-activating, cold-removing and pain-relieving ointment and a preparation method thereof. The analgesic ointment is prepared by taking black sesame oil and yellow lead as matrixes and assisting coarse medicinal materials with the effects of warming yang, dispelling cold, promoting blood circulation and dredging collaterals and fine medicinal materials (musk, camphor, myrrh, frankincense and the like) through the steps of treating the medicinal materials, extracting, refining oil, adding the yellow lead to form the ointment, removing fire toxin, adding the fine materials, spreading and the like. The traditional Chinese medicine composition does not contain western medicine components, can warm yang and dredge vessels, dispel cold and wind and eliminate dampness, promote blood circulation to remove meridian obstruction and remove blood stasis, and relieve swelling and pain, and is suitable for treating various orthopedic and rheumatism diseases such as acute and chronic lumbar and cervical diseases, knee joint hyperosteogeny, sciatica and scapulohumeral periarthritis. The ointment is large in drug loading capacity, capable of being administrated for a long time, good in long-term curative effect, not prone to relapse after healing, capable of being directly applied without heating, convenient to use, free of deterioration in two years at the normal temperature, small in irritation, low in anaphylaxis and high in safety.

The invention patent is titled "Dragon and Cypress Gel," belonging to the pharmaceutical category. The main components of Dragon and Cypress Gel are: Phellodendron bark, earthworm, dragon's blood, Oldenlandia diffusa, Sophora flavescens, and Bletilla striata. In recent years, research on cervicitis and cervical lesions has shown good results using external treatments of traditional Chinese medicine. Vaginal cervical medications are characterized by high local drug concentration, low dosage, and safety and effectiveness, receiving high clinical evaluation. The vagina and cervix have abundant capillaries and lymphatic vessels, and lack clearly defined nerve endings, resulting in minimal pain stimulation for patients during administration. This makes it an effective drug release site for specific diseases such as cervicitis and vaginitis. Traditional Chinese medicine gels are a novel type of external preparation of traditional Chinese medicine. They are made by dissolving or uniformly dispersing traditional Chinese medicine in a gel. These gels adhere tightly to the treatment site for a relatively long time, exhibiting good bioadhesion, and are simple to prepare. It is suitable for use on skin, mucous membranes and cavities. It not only avoids the first-pass effect of enzymes and acids in the gastrointestinal tract that exists in oral administration, but also reduces the adverse reactions of drugs. It has the characteristics of good spreadability, non-greasy feeling, easy to wash off, good transdermal absorption, and comfortable use.

The invention discloses a nano vaccine for treating HPV (human papilloma virus) infection related cervical lesions as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. In order to solve the problem that the response rate of an existing cervical lesion treatment vaccine does not meet clinical requirements, the hybrid membrane with enhanced immunogenicity and improved safety is prepared by applying a membrane fusion technology and combining an autologous tumor cell membrane and an escherichia coli cytoplasmic membrane; meanwhile, PLGA nano particles loaded with HPV16 E5 / E6 / E7 specific CTL epitope long peptide are used as a delivery carrier, and the HM-NPs-LP nano vaccine co-delivering the hybrid membrane and the HPV16 specific antigen peptide is developed. According to the nano vaccine, tumor cells and HPV16 E5 / E6 / E7 specific CTL epitope long peptide are taken as antigens for immunotherapy, dual immune activation of broad spectrum and specific antigens can be realized, personalized multi-effect treatment capacity for HPV infection related cervical diseases is exerted, targeted killing of HPV infection and pathological cells is enhanced, pathological relapse is prevented, and the nano vaccine has good clinical application prospects. And a new strategy is provided for individualized precise immunotherapy.

The present application relates to the technical field of medical data processing, and particularly relates to a cervical disease two-stage dynamic risk prediction method based on multidimensional data, comprising the following steps: step S1, data acquisition and structuring: a plurality of dimensions of patient data are collected and structured by questionnaire, and a cervical disease database is constructed; step S2, first-stage model construction: for a general screening population, a first prediction model is constructed by using the questionnaire survey data collected in step S1; step S3, second-stage model construction: for a population with HPV positive or other suspicious indications obtained by the first prediction model, high-dimensional clinical data collected in step S1 are integrated, and a second prediction model is constructed by using an ensemble learning strategy; step S4, model series early warning output; and step S5, decision support generation. The present application integrates low-cost and easily-obtained multidimensional data, constructs a scientific risk stratification model, and realizes two-stage and continuous dynamic early warning.

The invention relates to the technical field of biological detection, and discloses a reagent composition for cervical cancer detection, a kit and application. The reagent composition comprises a reagent for detecting the methylation level function of cervical cancer marker genes or fragments thereof, and the marker genes comprise a PAUPAR gene, an NETO1 gene and an NTM gene. According to the reagent composition, the methylation level of a specific marker gene combination is detected, efficient non-invasive screening of high-grade cervical lesions and cervical cancer can be achieved, and high detection specificity and sensitivity are achieved.

Devices, systems and methods are provided for treating conditions of the reproductive tract. A number of conditions can afflict the lining and cell layers deeper within the anatomical structures. For example, cervical intraepithelial neoplasia (CIN), also known as cervical dysplasia, is a condition involving abnormal growth of cells on the surface of the cervix that could potentially lead to cervical cancer in situ (CIS). Other conditions include human papillomavirus (HPV)-related cervical disease, various endometrial diseases, acute and chronic cervicitis, and various infections (e.g. trichomoniasis) to name a few. In some embodiments, treatments eliminate diseased, damaged, abnormal or otherwise undesired cells leaving the tissue framework intact. This allows the tissue to regenerate in a normal fashion, avoiding the formation of scar tissue. When the tissue framework is left intact, the framework structure repopulates with healthy cells, regenerating the normal tissue without altering the structural properties.

The invention belongs to the technical field of medical animal model construction, and particularly relates to a construction method of an animal model for simulating various stages from HPV (human papillomavirus) continuous infection to cervical canceration in a natural infection state and application of the animal model. According to the method, a drug immunosuppressor for promoting HPV infection is combined with MmuPV1 for mouse lower genital tract infection modeling; the constructed animal model is stable and uniform in papilloma virus infection, high in modeling rate, low in fatality rate, short in modeling time, controllable in process and good in reproducibility, can completely reproduce the whole process from HPV continuous infection to cervical canceration, accords with clinical practice, improves the efficiency and quality of model construction, and is suitable for popularization and application. An ideal animal model is provided for follow-up research on an HPV infection carcinogenic mechanism and a virus host interaction mechanism, meanwhile, the model can assist in research, development and screening of clinical treatment methods for preventing and treating cervical lesions at all stages, and clinical transformation, new drug research and development and other work are promoted.

The present application relates to the technical field of image recognition model algorithm, in particular to a kind of colposcope intelligent auxiliary diagnosis decision system construction method, step S1, with multi-modal cervical disease database as the basis, the image data of different lesion degrees is widely collected, the image data is annotated by experienced doctor, and the high-risk area of high-grade cervical lesion is accurately demarcated to form target detection annotation dataset;Step S2, data preprocessing is carried out to the data obtained in step S1, including systematic cleaning and enhancement processing to the original image, and lesion area detection processing is carried out to the preprocessed data;Step S3, the candidate area detected in step S2 is input into feature learning network.The method of the present application fully considers the spatial distribution characteristics of lesion severity, preferentially recommends the area most likely to exist HSIL, thereby improving the targeting and diagnostic efficiency of biopsy.

The invention discloses a group of gene methylation primer probe compositions for auxiliary identification of cervical lesions, a kit, application and a diagnosis model, and relates to the technical field of biological detection, and the primer probe compositions are related to six genes of PAX1, SOX1, EPB41L3, ST6GALNAC5, SOX14 and ZNF671. The kit comprises a nucleic acid extraction or purification reagent, a methylation detection sample pretreatment reagent, a PCR reaction mixed solution, a primer probe mixed solution 1, a primer probe mixed solution 2 and a positive and negative quality control. The composition and the kit are used for detecting HPV negative or HPV positive cervical high-grade intraepithelial neoplasia, cervical squamous carcinoma and cervical adenocarcinoma. According to the invention, the detection sensitivity and specificity can be improved, the sample input amount is reduced, and multiple detections of one tube of sample in cervical cancer screening are realized; and different histopathological types of cervical cancer can be detected.

The invention relates to the technical field of cervical lesions, and discloses a cervical lesion risk prediction model based on combination of double gene methylation and clinical characteristics and application thereof, and the method comprises the following steps: S1, selecting a research object; s2, carrying out double-gene methylation detection; and S3, data statistical analysis: performing statistical analysis by applying SPSS 25.0 and R4.1. 2 software, performing chi-square test and single-factor logistic regression analysis on each potential influence factor, and bringing the screened influence factors into multi-factor logistic regression analysis. According to the cervical lesion risk prediction model based on combination of double-gene methylation and clinical characteristics and application and development thereof, evidence-based basis can be better provided, clinical reduction of missed diagnosis and misdiagnosis rates of cervical cancer is helped, morbidity and mortality are reduced, and implementation of a strategic target of eliminating cervical cancer in China is assisted.

The invention relates to the technical field of gynecological medical instruments, in particular to a gynecological cervical disease examination sampling device which comprises a first shell and a sampling assembly, an opening is formed in the first end of the first shell, the sampling assembly is arranged at the second end of the first shell, the sampling assembly comprises a driving rod, and the first end of the driving rod is fixedly connected with a sampling brush head; and the second end part of the driving rod is fixedly connected with the position adjusting assembly. The position adjusting assembly comprises a bearing disc and a rotary position adjusting rod, the driving rod is connected with the center of the side wall of the bearing disc, the rotary position adjusting rod is arranged on the edge of the side wall of the side, away from the driving rod, of the bearing disc, and a rotationally-connected sleeve is arranged at the end, away from the bearing disc, of the rotary position adjusting rod. When the sampling device is used, after an operator holds the sleeve, the driving rod and the sampling brush head can be driven to continuously rotate by rotating the position adjusting rod and the bearing disc, and the hand of the operator can do circular motion around the driving rod, so that the sleeve does not need to be loosened, the physical strength of the operator is saved, and the working efficiency of the sampling device is improved.

The application discloses application of VAMP8 in preparation of products for diagnosing and treating HPV16 virus infection related cervical diseases. Through research on expression and functions of VAMP8 in HPV16 infection and cervical disease progression, the application provides a new potential marker and treatment target for prevention and treatment of HPV16 related cervical diseases, and has important clinical application value. Compared with the prior art, the application can more accurately treat HPV16 infection and induced cervical diseases, reduce side effects, and improve treatment effect.

The present application relates to the technical field of biological detection, and discloses a reagent composition, a kit and application for detecting cervical cancer and precancerous lesions. MDGA2 gene, ZNF773 gene, CSDAP1 gene and CLEC14A gene. The reagent composition of the present application can realize efficient non-invasive screening of high-grade cervical lesions and cervical cancer by detecting the methylation level of a specific combination of marker genes, and has high detection specificity and sensitivity.

The present application belongs to the field of biological medicine, and particularly relates to the use of recombinant humanized collagen type XVII in the treatment of cervical cancer, and more particularly to the use of recombinant humanized collagen type XVII in the preparation of a drug for preventing and / or treating cervical diseases; the recombinant humanized collagen type XVII comprises or consists of a sequence represented by (A)m, wherein each A is selected from the amino acid sequence represented by any one of SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3; m is an integer between 1 and 10; each A is the same or different, and two adjacent As are directly connected by a peptide bond or connected by one or more amino acid residues. Research data shows that the recombinant humanized collagen type XVII can significantly inhibit the proliferation, migration, invasion and tumorigenicity of cervical cancer cells, and the inhibitory effect is dose-dependent.

The application discloses application of VAMP8 in preparation of products for diagnosing and treating HPV16 virus infection related cervical diseases. Through research on expression and functions of VAMP8 in HPV16 infection and cervical disease progression, the application provides a new potential marker and treatment target for prevention and treatment of HPV16 related cervical diseases, and has important clinical application value. Compared with the prior art, the application can more accurately treat HPV16 infection and induced cervical diseases, reduce side effects, and improve treatment effect.

The invention discloses an endometrial stem cell preparation for cervical hypertrophy and chaff scale repair and application, and belongs to the technical field of stem cells, the endometrial stem cell preparation comprises a stem cell gel carrier, endometrial stem cells, auxiliary repair polypeptide and a survival rate enhancer, in the endometrial stem cell preparation, the stem cell gel carrier is synergistically matched with the endometrial stem cells, the auxiliary repair polypeptide and the survival rate enhancer, so that the survival rate of the endometrial stem cells in vivo can be increased, and the auxiliary repair polypeptide can activate the MAPK / ERK pathway of the endometrial stem cells, so that the endometrial stem cells can be effectively repaired. The anti-inflammatory mimic peptide in the survival rate enhancer can inhibit stress silencing of the endometrial stem cells, so that the secretion function of the endometrial stem cells is ensured, and the targeted repair capacity of the endometrial stem cells is enhanced through the three-dimensional porous structure of the stem cell gel carrier; homing and colonization of the endometrial stem cells on the cervical diseased region are enhanced.

The invention belongs to the technical field of biological medicines, and discloses MMP12 as a biological marker for cervical cancer and precancerous lesions of cervical cancer and application of the MMP12 in order to solve the problem that a TIME change mechanism is still unclear in the progress process of cervical cancer at present. According to the application disclosed by the invention, the increase of MMP12 positive macrophages along with the progress of the cervical disease is determined for the first time, the MMP12 protein secreted by the MMP12 positive macrophages is a specific molecular characteristic of the progress of the cervical disease, and a new basis is provided for accurate diagnosis and treatment. Through multi-omics analysis and in-vivo and in-vitro functional experiments, it is proved that the MMP12 protein can drive growth, invasion and migration of cervical cancer tumors and shape an immunosuppression microenvironment, and the MMP12 protein is a treatment target with great potential. The effect of the MMP12 inhibitor is verified in an in-vivo and in-vitro model for the first time, and an experimental basis is provided for a treatment strategy of cervical cancer and precancerous lesions of cervical cancer.