52 results about "Cell staining" patented technology

The embodiment of the invention relates to the technical field of drug detection, in particular to an anti-CD38 antibody drug receptor occupation detection method and system, a peripheral blood sample is used for preparing a single-cell suspension, the single-cell suspension is divided into a TEST tube and an FMO tube, a cell surface antibody and an RO detection antibody are added into the TEST tube, only the cell surface antibody is added into the FMO tube, and only the RO detection antibody is added into the FMO tube. The TEST tube and the FMO tube are subjected to sample detection analysis, so that the average fluorescence intensity of RO detection antibodies of the TEST tube and the FMO tube is obtained. And performing RO detection analysis by adopting a dissociation method, taking the tubes which are not combined with the medicine as a total receptor group, taking the tubes which are combined with the medicine as a dissociation receptor group, and calculating an RO detection result according to the average fluorescence intensity of the TEST tube and the FMO tube.

According to the cell fixing liquid, phenol oxidase is used for catalyzing phenol to be oxidized into a quinone structure, the quinone structure reacts with protein amido to form light covalent immobilization, cell immobilization is achieved, a stable hydration environment and membrane protection are provided through the ionic liquid solvent choline dihydrogen phosphate, a phosphate-free buffer system is provided, and cell immobilization is achieved. Therefore, a novel cell fixing liquid is obtained, the cell structure integrity required by cell imaging analysis is effectively reserved, meanwhile, cell staining fluorescence signal interference is avoided, and therefore the accuracy of cell counting and cell imaging analysis is ensured.

An imaging flow cytometry-based high-throughput drug screening method, comprising: cell incubation, cell staining, acquiring single-cell images by using a flow cytometer, and image extraction and analysis, which combines the high-throughput advantages of flow cytometry and the imaging capability of a microscope, so that multi-channel single-cell images can be generated in a high throughput manner, thereby implementing acquisition of fluorescent images and unmarked images of single cells at a throughput of 102-105 cells per second, which can be used for cell phenotype drug screening and improving the screening efficiency by a factor of 102-104.

The invention discloses an image processing method and system based on an in-situ hybridization technology and a medium, and relates to the technical field of biological information, and the method comprises the following steps: converting a cell DAPI dyeing result into two-dimensional data from three-dimensional data by utilizing Z-axis maximum intensity projection, identifying and separating a single cell from the two-dimensional data by utilizing cell segmentation, and when the cell segmentation is used for processing an overlapping region, identifying and separating the single cell from the two-dimensional data. Using a registration algorithm Ashlar to calculate an error between adjacent visual fields of the same round to obtain cell position information; an imaging result after hybridization of the fluorescent probe and the gene is subjected to multiple rounds of registration through a registration algorithm Ashlar to generate a spliced image, local maximum values of the spliced image under different rounds are marked by using a fluorescent dot recognition algorithm, so that position information of each fluorescent dot is obtained, the position information of each fluorescent dot corresponds to the gene in a transcript, and the position information of each fluorescent dot corresponds to the gene in the transcript. Obtaining gene position information; distributing genes into cells by utilizing the cell position information and the gene position information to obtain a cell gene matrix and displaying the cell gene matrix; according to the image processing method and system and the medium, accurate matching of sequencing data and spatial information is achieved, and then the distribution rule of gene expression in space is better revealed.

The invention discloses cell enriching and dyeing integrated equipment which comprises a base; the enrichment assembly is arranged on the base and is used for enriching target cells; the dyeing assembly is arranged on the base and is used for dyeing target cells; the liquid adding assembly comprises a first guide rail arranged on the base, a first liquid adding pipe and a second liquid adding pipe, the first liquid adding pipe and the second liquid adding pipe are in sliding fit with the first guide rail, the first liquid adding pipe is used for adding a first reagent to the enrichment assembly, and the second liquid adding pipe is used for adding a second reagent to the dyeing assembly. The enrichment assembly and the dyeing assembly are integrated on the same base, the liquid adding assembly with the first liquid adding pipe and the second liquid adding pipe which can slide along the guide rail is arranged, the guide rail design of the liquid adding assembly ensures the accuracy and repeatability of liquid adding, mechanical operation of reagent adding is achieved, and the reagent adding efficiency is improved. The time and steps for manually taking and placing the reagent are obviously reduced, so that the efficiency of the whole cell enrichment, dyeing and subsequent identification process is effectively improved.

Provided are a single-cell chromatin accessibility and transcriptome combined sequencing method and application thereof. The method comprises: treating a cell nucleus using a transposase to obtain gDNA of a chromatin open region connected with a first specific tag sequence; treating the cell nucleus using a transcriptome capture sequence to obtain cDNA connected with a second specific tag sequence; generating a droplet based on a droplet microfluidic to seal the cell nucleus and a first microbead in the droplet, wherein the gDNA and the cDNA are captured by the first microbead, and wherein more than one cell nucleus is sealed in the droplet; and sequencing the gDNA and the cDNA to obtain combined information of single-cell chromatin accessibility and transcriptome according to the first specific tag sequence and the second specific tag.

Provided herein are, inter alia, are methods and systems for amplifying a chromosomal fragment DNA sequence. The methods and systems provided herein are, inter alia, useful for detecting copy number variation, structural variation, and / or extrachromosomal DNA within the chromosomal DNA of a plurality of cells. In addition, the methods provided herein are, inter alia, useful for jointly analyzing chromatin architecture and gene expression at a single cell level. The methods provided herein can be, inter alia, used to analyze chromosomal heterogeneity, clonal shifts, and / or evolutionary shifts between different cells.

This invention discloses a mesenchymal stem cell detection device, relating to the field of cell detection technology. It includes a detection unit comprising an operating table, a staining microscope mounted on top of the operating table, and a display screen mounted on top of the operating table. The operator places a culture dish on top of a fixing component and fixes it using a linkage component. A vibration component drives the linkage and fixing components, causing the culture dish to rotate sequentially to the bottom of the staining microscope for detection. The detection results are displayed on the display screen in real time. By using two staining microscopes, two culture dishes can be detected simultaneously and their data compared, ensuring the accuracy of the detection data. During cell staining, the rapid rotation of the vibration component promotes the mixing of the staining agent with the cells, reducing the staining mixing time.

The application relates to the technical field of image processing, in particular to an abnormal cell screening method and system based on image analysis, which comprises the following steps: acquiring continuous image frames of a staining process, extracting average optical density, integral optical density and chromatin gray standard deviation of sample cells as staining parameters, and acquiring chromatin texture; forming a staining parameter space curve with the change of the staining parameters over time, fitting to obtain a continuous staining curve; aligning and fitting the continuous staining curve with a standard parameter curve calibrated in advance to obtain a coordinate transformation vector; acquiring an image frame after the completion of the staining, performing standardization correction on the staining parameters of the to-be-tested cells by using the coordinate transformation vector to obtain chromatin texture features, and then performing feature comparison and identification on abnormal cells. The application reduces the staining sensitivity difference caused by biological factors such as individual gene expression difference, different cell membrane protein compositions and pH value changes, and eliminates the influence of staining condition difference and equipment difference among different laboratories on the screening result.

The application provides an immunohistochemical staining image analysis method, including: cutting a plurality of cell nuclei of an immunohistochemical staining image according to a machine learning model; removing pixels belonging to the cell nuclei in the immunohistochemical staining image and removing pixels meeting a color range to obtain a plurality of cytoplasm pixels; distributing the cytoplasm pixels to the cell nuclei according to the positions of the cytoplasm pixels to form a plurality of cells; and calculating a pixel staining score of each pixel in the cells, thereby calculating a cell staining score of the cells. Thus, the staining score of the cells can be objectively and automatically calculated.

The invention belongs to the technical field of biomedical detection, and discloses a blood cell analysis system based on image flow cytometry, which comprises a cell staining module, the dyeing module simultaneously comprises a first fluorescent probe capable of being specifically combined with components in a cell nucleus or enriched in the cell nucleus, a second fluorescent probe capable of being specifically combined with cell nucleus DNA and a third fluorescent probe capable of being specifically combined with a blood cell differentiation antigen; an image flow cytometer; and the image processing module is used for calculating the kernel detection rate and / or the average kernel number. According to the present invention, the combination of the specific types of the cell staining dyes is adopted, the image flow cytometry is matched, and the optimized multi-dimensional fluorescence staining combination and the matched automatic image analysis process are adopted to achieve the synchronous, high-throughput and accurate quantitative analysis of the blood cell immune phenotype and the cell morphology key characteristics at the single cell level;

The invention provides an artificial intelligence umbilical cord stem cell quality detection data analysis method and system, and relates to the technical field of cell quality detection.The method comprises the steps that umbilical cord stem cell dyeing monitoring parameters are obtained and compared with umbilical cord stem cell dyeing expected parameters, and umbilical cord stem cell dyeing parameter deviation vectors are obtained; a first umbilical cord stem cell activity detection sample set is collected, centralized value evaluation is executed, and an umbilical cord stem cell activity value confidence interval is obtained; when the umbilical cord stem cell activity detection value does not belong to the umbilical cord stem cell activity value confidence interval, carrying out abnormal quality detection data identification on the umbilical cord stem cell activity detection value; and when the umbilical cord stem cell activity detection value belongs to the umbilical cord stem cell activity value confidence interval, performing normal quality detection data identification on the umbilical cord stem cell activity detection value. The technical problem of poor stability of umbilical cord stem cell quality detection results in the prior art is solved.

The utility model provides a kind of floating type's vibration mixing device and cell staining equipment, floating type's vibration mixing device includes slide loading and unloading mechanism, vibration motor, height adjusting mechanism and base, slide loading and unloading mechanism includes slide rack, glass slide and limit post, installation groove is opened on slide rack, glass slide is set in installation groove, slide rack front part is equipped with limit block, glass slide one end abuts limit block, limit post is set in the both sides and rear portion of slide rack, vibration motor is fixedly arranged below slide loading and unloading mechanism, height adjusting mechanism is located between slide loading and unloading mechanism and base.The device simple structure, there is no any shelter above slide, it is convenient for operator to load and unload glass slide.

The invention relates to the technical field of biomedical detection, in particular to a multiple myeloma circulating plasma cell flow cytometry method and system, a peripheral blood sample is used for preparing a single-cell suspension, the single-cell suspension is extracted to prepare a to-be-detected tube (TEST tube), the TEST tube is subjected to non-specific site sealing, and the TEST tube is subjected to non-specific site sealing to obtain the multiple myeloma circulating plasma cell flow cytometry method and the multiple myeloma circulating plasma cell flow cytometry system. The method comprises the following steps: taking a TEST tube as a substrate, adding a multi-fluorescent antibody panel containing nano antibodies aiming at BCMA and GPRC5D to perform cell staining operation, performing flow cytometry analysis on the TEST tube to obtain flow cytometry data, and performing gate analysis on the flow cytometry data according to a preset detection analysis strategy to obtain a fluorescence detection result. The number and phenotypic characteristics of circulating plasma cell (CPC) populations are identified and counted, so that circulating plasma cell detection information is generated.

The invention relates to the technical field of image processing, in particular to an abnormal cell screening method and system based on image analysis, and the method comprises the steps: obtaining continuous image frames of a dyeing process, extracting the average optical density, integral optical density and chromatin gray standard deviation of sample cells as dyeing parameters, and obtaining chromatin textures; the dyeing parameters are changed along with time to form a dyeing parameter space curve, and a continuous dyeing curve is obtained through fitting; aligning and fitting the continuous dyeing curve and a pre-calibrated standard parameter curve to obtain a coordinate transformation vector; and obtaining a dyed image frame, carrying out standardized correction on a to-be-detected cell dyeing parameter by using the coordinate transformation vector to obtain a chromatin texture feature, and carrying out feature comparison to identify an abnormal cell. According to the method, the dyeing sensitivity difference caused by biological factors such as individual gene expression difference, cell membrane protein composition difference and pH value change is reduced, and the influence of dyeing condition difference and equipment difference among different laboratories on screening results is eliminated.

The application discloses a method for simultaneously identifying cell subgroups and related factor phosphorylation levels. The method simplifies the protein phosphorylation flow cytometry staining step without affecting the staining effect, can simultaneously detect 17 stained cells, and simultaneously saves cost and is more efficient. The method provided by the application can provide more accurate distinguishing ability, can obtain more information from a small cell sample, can detect abnormal cells at a low level, increases laboratory efficiency, is especially suitable for the research of the immune system, and has a wide prospect.

The invention discloses a folate receptor mediated cell staining solution and a preparation method and application thereof, and the folate receptor mediated cell staining solution comprises the following components by weight: 0.05%-0.2% of methylene blue, 0.3%-1% of ascorbic acid, 0.3%-1% of folic acid, 1%-3% of acetic acid, 0.1%-0.4% of sodium acetate, 1%-3% of propylene glycol, 0.1%-0.5% of sodium diphenylaminesulfonate, and the balance of water. According to the sodium diphenylaminesulfonate, the cell staining solution can better distinguish different stages of tumors, so that the sensitivity and the accuracy of a detection result are ensured. In addition, the prepared staining solution is long in storage time, the detection time is shortened to a great extent, and the detection mode is simplified to a great extent.

The invention provides a construction method and application of an anti-myocardial damage screening model based on intelligent recognition, and belongs to the field of drug screening models. The mitochondrial active oxygen level is indicated through the mitochondrial superoxide indicator, the mitochondrial morphological parameters are indicated through the mitochondrial dark red fluorescent probe, and the DNA damage level is indicated through Hoechst; the method comprises the following steps: performing high-content imaging after cell staining, analyzing the mitochondrial active oxygen level by adopting a high-content MetaXpress built-in module, analyzing mitochondrial morphological parameters by adopting Image pro plus software, analyzing the DNA foci quantity by adopting a FociNet model to obtain a high-content multichannel image, and further obtaining the spatial representation of a to-be-detected compound through Microsnop image learning. The screening model can accurately distinguish different action mechanisms of compounds, presents specific clusters consistent with phenotypic characteristics in a characteristic space, is high in flux and specificity, and can be used for screening anti-adriamycin myocardial injury positive drugs.

The invention discloses a method and a system for synchronously detecting intracellular microbial colonization and host response at a single cell level, and belongs to the technical field of single cell epimics and microbiomics. Microorganism colonization information and host chromatin openness information of each single cell are synchronously extracted from single cell chromatin accessibility sequencing data, and correlation analysis is performed on the microorganism colonization information and the host chromatin openness information at the single cell level, so that a cell specificity mechanism of host-microorganism interaction is analyzed. At present, a systematic method capable of synchronously realizing microbiological detection and host chromatin accessibility analysis under single cell resolution is not reported yet, and the method fills the technical blank. The method can solve the problem that the existing sequencing technology based on 16S rRNA, 18S rRNA or scRNA-seq is difficult to analyze microorganism colonization and host chromatin accessibility synchronously and highly sensitively at the single cell level, and provides an innovative tool for revealing a host-microorganism interaction mechanism of cell specificity in complex tissues.

The utility model belongs to the technical field of cell staining, and particularly relates to a cell staining device which comprises a working table, the top of the working table is fixedly connected with a supporting frame, and one side of the supporting frame is connected with a first motor. According to the cell dyeing device, the supporting frame, the first motor, the screw rod, the movable block, the flow dividing pipe, the dropper, the connecting pipe, the stirring barrel, the second motor, the stirring rod, the water inlet pipe and the water outlet pipe are matched for use, so that the device can dye a plurality of groups of cells when being used by a worker, and the working efficiency is improved when the worker uses the device. According to the cell staining device, the problems that a staining agent is layered after standing for a long time, a plurality of groups of cells cannot be stained, and the staining agent on the glass slide needs to be manually and uniformly smeared are solved, so that the staining agent is not layered after standing for a long time, and the plurality of groups of cells can be stained; and the staining agent on the glass slide can be automatically and uniformly smeared.

The invention relates to a sample treatment method for detecting accessibility of single cell chromatin and application of the sample treatment method. The sample treatment method comprises the following steps: slicing a tissue sample to obtain a tissue slice, and sequentially carrying out permeabilization treatment, transposase treatment and cell nucleus extraction treatment on the tissue slice, the tissue sample includes a frozen tissue sample. According to the method, a'slice in-situ transposition reaction 'strategy is designed, the original spatial conformation and physiological state of chromatin in vivo are effectively maintained, chromatin structure disturbance caused by early release of cell nucleuses in the thawing process is avoided, the quality of ATAC-seq data is remarkably improved, and the method is suitable for being used in a large scale. The enrichment fraction of the transcription start site and the proportion result of fragments overlapped with peaks are remarkably improved, a nucleosome positioning signal is clearer, and the accuracy and reliability of chromatin openness detection are improved.

Compositions, methods, and kits for performing multiplexed, spatially resolved, or single-cell chromatin analysis are provided.

The invention belongs to the technical field of biology, and discloses a living cell chromatin ring reporting system and application thereof. The living cell chromatin ring reporting system comprises a DNA sequence for coding CTCC-fluorescent protein C-terminal or N-terminal fusion protein and coding Cohesin compound subunit and fluorescent protein N-terminal or C-terminal fusion protein. The reporting system does not influence the original function of CTCF-Cohesin, can visually monitor the formation of CTCF-Cohesin mediated chromatin Loop in real time, has high structural specificity and temporal-spatial resolution, and is obviously superior to the traditional Hi-C or ChIA-PET and other analysis methods which are only limited to endpoint fixed samples. By constructing a stably expressed fusion protein cell line, the signal consistency and the system robustness can be further improved, and the method is suitable for large-scale screening application. Strategies such as small molecule treatment, CRISPR (clustered regularly interspaced short palindromic repeats) libraries and transcription factor function verification can be combined, research means of chromatin structure regulation mechanisms can be expanded, and discovery of novel epigenetic regulation factors can be accelerated.

1. Name of the product in this design: Liquid-based thin-layer cell preparation machine. 2. Purpose of this design: This design is used for cell staining of histological specimens. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

1. The name of the design product: upright fluorescent module. 2. The use of the design product: the design product is mainly used in the field of life science research, cell chromosome identification, biomedical observation and other fluorescent substance detection, and can be used with a microscope. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

1. The name of the design product: upright fluorescent module (DD-3). 2. The use of the design product: the design product is mainly used in the fields of life science research, cell chromosome identification, biomedical observation and other fluorescent substance detection, and can be used with a microscope. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model discloses a liquid-based cell staining and slide-making all-in-one machine, which relates to the field of cell staining and slide-making, and comprises a liquid-based cell staining and slide-making machine, a slide-making machine side shell, a bottom inner cavity, a top inner cavity, a centrifugal disc body, a sample disc body and a slide-making machine body, a staining reagent bottle is arranged in the liquid-based cell staining tableting machine, and a staining reagent replacement bottle can be selected as the staining reagent bottle. The liquid-based cell staining tableting machine solves the problem that liquid cannot be added and discharged due to adhesion of a pipeline after corrosion caused by absence of water and waste liquid; and the high-precision sample needle is matched with the ceramic pump to accurately control the sample adding amount of a dyeing reagent and a sample by adopting a dripping dyeing technology. The sample adding needle and the dyeing needle both adopt a drop dyeing technology, so that a traditional injector sample adding mode is abandoned, the problem that the needle head of the sample adding needle is blocked by crystals due to the fact that a dyeing reagent is not used for a long time is fundamentally solved, and the dyeing effect is better.

The utility model provides a biological cell dyeing device relates to cell dyeing technical field, including base, the base upper surface one side is provided with a plurality of layers horizontal placement's superposition board, the superposition board upper surface four corner all fixed mounting has the socket and superposition board lower surface is located in any socket just below position all fixed mounting has the plug -in post, through setting up superposition board, and through setting up first jack, second jack and third jack of different distribution size on superposition board, utilize the plugging of front fender insert piece and rear fender insert piece and first jack, second jack or third jack realizes the relative position adjustment, and it is convenient for placing different size's object glass on superposition board, and utilize the resistance of front fender insert piece and rear fender insert piece to object glass corner position can fix the position of object glass.

The cell staining device frame comprises a base, a liquid storage box, stand columns, a clamping structure and a limiting rod, the top and one side of the base are of an opening structure, the liquid storage box is connected into the base in a sliding mode, water absorption sponge is laid at the bottom of the liquid storage box, and the stand columns are arranged at the two ends of the two sides of the base. First movable grooves are formed in the opposite sides of the two stand columns at the ends, away from the open end of the base, of the stand columns, the clamping structures are arranged between the two stand columns on the same side, and the limiting rods are arranged between the stand columns on the two sides close to the open end of the base and rotationally connected with the stand columns. Compared with the prior art, the glass slide clamping device has the advantages that the clamping rods are extruded through the springs when the glass slides are clamped, so that the glass slides with different widths can be clamped, and due to the fact that the clamping grooves are of the V-shaped structures, the glass slides with different thicknesses can be clamped.