8 results about "Cell staining" patented technology

Provided are a single-cell chromatin accessibility and transcriptome combined sequencing method and application thereof. The method comprises: treating a cell nucleus using a transposase to obtain gDNA of a chromatin open region connected with a first specific tag sequence; treating the cell nucleus using a transcriptome capture sequence to obtain cDNA connected with a second specific tag sequence; generating a droplet based on a droplet microfluidic to seal the cell nucleus and a first microbead in the droplet, wherein the gDNA and the cDNA are captured by the first microbead, and wherein more than one cell nucleus is sealed in the droplet; and sequencing the gDNA and the cDNA to obtain combined information of single-cell chromatin accessibility and transcriptome according to the first specific tag sequence and the second specific tag.

The utility model provides a kind of floating type's vibration mixing device and cell staining equipment, floating type's vibration mixing device includes slide loading and unloading mechanism, vibration motor, height adjusting mechanism and base, slide loading and unloading mechanism includes slide rack, glass slide and limit post, installation groove is opened on slide rack, glass slide is set in installation groove, slide rack front part is equipped with limit block, glass slide one end abuts limit block, limit post is set in the both sides and rear portion of slide rack, vibration motor is fixedly arranged below slide loading and unloading mechanism, height adjusting mechanism is located between slide loading and unloading mechanism and base.The device simple structure, there is no any shelter above slide, it is convenient for operator to load and unload glass slide.

Compositions, methods, and kits for performing multiplexed, spatially resolved, or single-cell chromatin analysis are provided.

The utility model provides a biological cell dyeing device relates to cell dyeing technical field, including base, the base upper surface one side is provided with a plurality of layers horizontal placement's superposition board, the superposition board upper surface four corner all fixed mounting has the socket and superposition board lower surface is located in any socket just below position all fixed mounting has the plug -in post, through setting up superposition board, and through setting up first jack, second jack and third jack of different distribution size on superposition board, utilize the plugging of front fender insert piece and rear fender insert piece and first jack, second jack or third jack realizes the relative position adjustment, and it is convenient for placing different size's object glass on superposition board, and utilize the resistance of front fender insert piece and rear fender insert piece to object glass corner position can fix the position of object glass.

The present application relates to the technical field of cell staining, and particularly relates to a vaginal secretion staining solution, a preparation method and a staining method thereof.The vaginal secretion staining solution provided by the present application uses basic fuchsin and methylene blue as staining agents, can stain epithelial cells and leukocytes, and cannot stain trichomonas and mold, so that leukocytes and trichomonas can be obviously distinguished through color difference, and personnel misjudgment caused by the similarity of leukocytes and trichomonas is reduced.The vaginal secretion staining solution provided by the present application has simple operation steps, short cycle and fast staining.The present application further provides a preparation method and a staining method of the vaginal secretion staining solution.

The application provides a single-cell protein high-resolution quantitative detection system and method based on a micro-droplet compression channel, and the system composition comprises: a micro-droplet lysis and generation module, which is used for realizing the falling of a single-cell dyeing antibody in a droplet; a micro-droplet quartz compression channel detection module, which is used for realizing the collection and detection of a single-cell protein fluorescence signal in a micro-droplet; a micro-droplet fluorescence signal collection and processing module, which is used for processing and analyzing the collected fluorescence signal; and a micro-droplet driving module, which is used for realizing the flow rate control of the micro-droplet in a micro-channel. The application reduces optical noise by using quartz instead of a common base material, reduces electrical noise by using a sine wave to control a laser source to modulate fluorescence, and has a significant two-order-of-magnitude improvement in detection resolution compared with existing methods; and the application realizes high reliability and accuracy of detection results by optimizing the composition of a cell lysis solution compared with existing methods.

This invention relates to the field of pathogen infection detection technology, specifically a method for visually tracking human bronchial organoids infected with Mycoplasma pneumoniae. It includes the following steps: (1) Constructing human bronchial organoids: culturing mature human bronchial organoids with cavities, the organoids comprising ciliated cells, basal cells, goblet cells, and Club cells, having a polarized three-dimensional structure with the apex facing outwards; (2) Mycoplasma pneumoniae culture and fluorescent labeling: Mycoplasma pneumoniae is amplified and cultured in MP complete medium, and then fluorescently labeled using CellTrace far-infrared cell staining solution; (3) Co-culture for infection: Mycoplasma pneumoniae fluorescently labeled in step (2) is co-cultured with the human bronchial organoids from step (1) for infection; (4) Live cell dynamic fluorescence tracking: Non-invasive in situ continuous imaging is performed on the human bronchial organoids infected by MP in step (3). This invention can achieve non-invasive dynamic tracking of the entire process of MP infection.