82 results about "Cell staining" patented technology

The invention provides a single-cell multi-omics cell type annotation method based on distribution and knowledge alignment, and belongs to the technical field of single-cell type annotation, the method comprises the following steps: obtaining single-cell transcriptome data and single-cell chromatin accessibility sequencing data, and pre-training and training a multi-omics variation auto-encoder model, the multi-omics variational auto-encoder model is combined with a variational auto-encoder and a knowledge distillation technology, and multi-omics single cell data is integrated and annotated through distribution and knowledge alignment. And performing cell type prediction on the single cell transcriptome data and the single cell chromatin accessibility sequencing data which are input at the same time by using the trained multi-omics variational auto-encoder model. According to the method, the problem of limitation of a method only depending on single omics is solved, the synergistic effect between the omics is enhanced, the accuracy of annotation is improved, and the calculation overhead is reduced through knowledge distillation.

The embodiment of the invention relates to the technical field of drug detection, in particular to an anti-CD38 antibody drug receptor occupation detection method and system, a peripheral blood sample is used for preparing a single-cell suspension, the single-cell suspension is divided into a TEST tube and an FMO tube, a cell surface antibody and an RO detection antibody are added into the TEST tube, only the cell surface antibody is added into the FMO tube, and only the RO detection antibody is added into the FMO tube. The TEST tube and the FMO tube are subjected to sample detection analysis, so that the average fluorescence intensity of RO detection antibodies of the TEST tube and the FMO tube is obtained. And performing RO detection analysis by adopting a dissociation method, taking the tubes which are not combined with the medicine as a total receptor group, taking the tubes which are combined with the medicine as a dissociation receptor group, and calculating an RO detection result according to the average fluorescence intensity of the TEST tube and the FMO tube.

According to the cell fixing liquid, phenol oxidase is used for catalyzing phenol to be oxidized into a quinone structure, the quinone structure reacts with protein amido to form light covalent immobilization, cell immobilization is achieved, a stable hydration environment and membrane protection are provided through the ionic liquid solvent choline dihydrogen phosphate, a phosphate-free buffer system is provided, and cell immobilization is achieved. Therefore, a novel cell fixing liquid is obtained, the cell structure integrity required by cell imaging analysis is effectively reserved, meanwhile, cell staining fluorescence signal interference is avoided, and therefore the accuracy of cell counting and cell imaging analysis is ensured.

An imaging flow cytometry-based high-throughput drug screening method, comprising: cell incubation, cell staining, acquiring single-cell images by using a flow cytometer, and image extraction and analysis, which combines the high-throughput advantages of flow cytometry and the imaging capability of a microscope, so that multi-channel single-cell images can be generated in a high throughput manner, thereby implementing acquisition of fluorescent images and unmarked images of single cells at a throughput of 102-105 cells per second, which can be used for cell phenotype drug screening and improving the screening efficiency by a factor of 102-104.

The invention discloses an image processing method and system based on an in-situ hybridization technology and a medium, and relates to the technical field of biological information, and the method comprises the following steps: converting a cell DAPI dyeing result into two-dimensional data from three-dimensional data by utilizing Z-axis maximum intensity projection, identifying and separating a single cell from the two-dimensional data by utilizing cell segmentation, and when the cell segmentation is used for processing an overlapping region, identifying and separating the single cell from the two-dimensional data. Using a registration algorithm Ashlar to calculate an error between adjacent visual fields of the same round to obtain cell position information; an imaging result after hybridization of the fluorescent probe and the gene is subjected to multiple rounds of registration through a registration algorithm Ashlar to generate a spliced image, local maximum values of the spliced image under different rounds are marked by using a fluorescent dot recognition algorithm, so that position information of each fluorescent dot is obtained, the position information of each fluorescent dot corresponds to the gene in a transcript, and the position information of each fluorescent dot corresponds to the gene in the transcript. Obtaining gene position information; distributing genes into cells by utilizing the cell position information and the gene position information to obtain a cell gene matrix and displaying the cell gene matrix; according to the image processing method and system and the medium, accurate matching of sequencing data and spatial information is achieved, and then the distribution rule of gene expression in space is better revealed.

Provided is a method and apparatus for cell staining without cell loss, and more particularly, to a method and apparatus for cell staining without cell loss during treatment of a staining reagent or washing reagent by immobilizing cells to be analyzed in a phase change material before staining. A method for cell staining without cell loss may effectively prevent cell loss during staining and analysis of rare cells that are becoming important in clinical diagnosis, and thus may facilitate observation, analysis and diagnosis thereby even with a trace amount of sample. In addition, as the method for cell staining without cell loss allows immobilization of cells, it makes discrimination and isolation of single cells easy, and may be effectively used in analysis of the isolated cells.

The utility model is applicable to the technical field of cell staining devices, and provides a bioscience cell staining device which comprises a plurality of placing seats, a driving mechanism, a staining unit and a positioning structure, the driving mechanism comprises a bottom plate, a first stepping motor, a mounting disc and a sleeve, and the positioning structure comprises a driving rod, a positioning plate, an inflation and deflation pump and an elastic rope. The bioscience cell staining device is provided with a plurality of placing seats, a driving mechanism, a staining unit and a positioning structure, the placing seats are driven by a driving machine to move to staining stations one by one, and the staining unit comprises an injection cylinder, a piston, a piston rod, a liquid suction pipe, a liquid outlet pipe and a power mechanism. The dyeing unit performs continuous dyeing operation, the automation degree is high, and the working efficiency is improved.

The invention discloses cell enriching and dyeing integrated equipment which comprises a base; the enrichment assembly is arranged on the base and is used for enriching target cells; the dyeing assembly is arranged on the base and is used for dyeing target cells; the liquid adding assembly comprises a first guide rail arranged on the base, a first liquid adding pipe and a second liquid adding pipe, the first liquid adding pipe and the second liquid adding pipe are in sliding fit with the first guide rail, the first liquid adding pipe is used for adding a first reagent to the enrichment assembly, and the second liquid adding pipe is used for adding a second reagent to the dyeing assembly. The enrichment assembly and the dyeing assembly are integrated on the same base, the liquid adding assembly with the first liquid adding pipe and the second liquid adding pipe which can slide along the guide rail is arranged, the guide rail design of the liquid adding assembly ensures the accuracy and repeatability of liquid adding, mechanical operation of reagent adding is achieved, and the reagent adding efficiency is improved. The time and steps for manually taking and placing the reagent are obviously reduced, so that the efficiency of the whole cell enrichment, dyeing and subsequent identification process is effectively improved.

Provided are a single-cell chromatin accessibility and transcriptome combined sequencing method and application thereof. The method comprises: treating a cell nucleus using a transposase to obtain gDNA of a chromatin open region connected with a first specific tag sequence; treating the cell nucleus using a transcriptome capture sequence to obtain cDNA connected with a second specific tag sequence; generating a droplet based on a droplet microfluidic to seal the cell nucleus and a first microbead in the droplet, wherein the gDNA and the cDNA are captured by the first microbead, and wherein more than one cell nucleus is sealed in the droplet; and sequencing the gDNA and the cDNA to obtain combined information of single-cell chromatin accessibility and transcriptome according to the first specific tag sequence and the second specific tag.

Provided herein are, inter alia, are methods and systems for amplifying a chromosomal fragment DNA sequence. The methods and systems provided herein are, inter alia, useful for detecting copy number variation, structural variation, and / or extrachromosomal DNA within the chromosomal DNA of a plurality of cells. In addition, the methods provided herein are, inter alia, useful for jointly analyzing chromatin architecture and gene expression at a single cell level. The methods provided herein can be, inter alia, used to analyze chromosomal heterogeneity, clonal shifts, and / or evolutionary shifts between different cells.

This invention discloses a mesenchymal stem cell detection device, relating to the field of cell detection technology. It includes a detection unit comprising an operating table, a staining microscope mounted on top of the operating table, and a display screen mounted on top of the operating table. The operator places a culture dish on top of a fixing component and fixes it using a linkage component. A vibration component drives the linkage and fixing components, causing the culture dish to rotate sequentially to the bottom of the staining microscope for detection. The detection results are displayed on the display screen in real time. By using two staining microscopes, two culture dishes can be detected simultaneously and their data compared, ensuring the accuracy of the detection data. During cell staining, the rapid rotation of the vibration component promotes the mixing of the staining agent with the cells, reducing the staining mixing time.

The application relates to the technical field of image processing, in particular to an abnormal cell screening method and system based on image analysis, which comprises the following steps: acquiring continuous image frames of a staining process, extracting average optical density, integral optical density and chromatin gray standard deviation of sample cells as staining parameters, and acquiring chromatin texture; forming a staining parameter space curve with the change of the staining parameters over time, fitting to obtain a continuous staining curve; aligning and fitting the continuous staining curve with a standard parameter curve calibrated in advance to obtain a coordinate transformation vector; acquiring an image frame after the completion of the staining, performing standardization correction on the staining parameters of the to-be-tested cells by using the coordinate transformation vector to obtain chromatin texture features, and then performing feature comparison and identification on abnormal cells. The application reduces the staining sensitivity difference caused by biological factors such as individual gene expression difference, different cell membrane protein compositions and pH value changes, and eliminates the influence of staining condition difference and equipment difference among different laboratories on the screening result.

The application provides an immunohistochemical staining image analysis method, including: cutting a plurality of cell nuclei of an immunohistochemical staining image according to a machine learning model; removing pixels belonging to the cell nuclei in the immunohistochemical staining image and removing pixels meeting a color range to obtain a plurality of cytoplasm pixels; distributing the cytoplasm pixels to the cell nuclei according to the positions of the cytoplasm pixels to form a plurality of cells; and calculating a pixel staining score of each pixel in the cells, thereby calculating a cell staining score of the cells. Thus, the staining score of the cells can be objectively and automatically calculated.

The invention belongs to the technical field of biomedical detection, and discloses a blood cell analysis system based on image flow cytometry, which comprises a cell staining module, the dyeing module simultaneously comprises a first fluorescent probe capable of being specifically combined with components in a cell nucleus or enriched in the cell nucleus, a second fluorescent probe capable of being specifically combined with cell nucleus DNA and a third fluorescent probe capable of being specifically combined with a blood cell differentiation antigen; an image flow cytometer; and the image processing module is used for calculating the kernel detection rate and / or the average kernel number. According to the present invention, the combination of the specific types of the cell staining dyes is adopted, the image flow cytometry is matched, and the optimized multi-dimensional fluorescence staining combination and the matched automatic image analysis process are adopted to achieve the synchronous, high-throughput and accurate quantitative analysis of the blood cell immune phenotype and the cell morphology key characteristics at the single cell level;

The invention provides an artificial intelligence umbilical cord stem cell quality detection data analysis method and system, and relates to the technical field of cell quality detection.The method comprises the steps that umbilical cord stem cell dyeing monitoring parameters are obtained and compared with umbilical cord stem cell dyeing expected parameters, and umbilical cord stem cell dyeing parameter deviation vectors are obtained; a first umbilical cord stem cell activity detection sample set is collected, centralized value evaluation is executed, and an umbilical cord stem cell activity value confidence interval is obtained; when the umbilical cord stem cell activity detection value does not belong to the umbilical cord stem cell activity value confidence interval, carrying out abnormal quality detection data identification on the umbilical cord stem cell activity detection value; and when the umbilical cord stem cell activity detection value belongs to the umbilical cord stem cell activity value confidence interval, performing normal quality detection data identification on the umbilical cord stem cell activity detection value. The technical problem of poor stability of umbilical cord stem cell quality detection results in the prior art is solved.

The invention discloses a method for confirming the cell mutation degree in a TCT detection mode. The method comprises the following steps: 1) preprocessing an original TCT cell staining image; 2) dividing pixels of the obtained grey-scale map into three categories of cell nucleuses, cell bodies and backgrounds, and respectively completing corresponding loop iterations; 3) performing segmentation, post-processing and closed operation in sequence from the obtained result maps of the three clusters, and determining a cell nucleus region result; 4) sequentially carrying out segmentation and post-processing operation in combination with the obtained cell nucleus region from the three obtained clustering result maps, and determining a cell body region result; and 5) according to the obtained cell nucleus region result and the obtained cell body region result, obtaining the nucleus-plasma ratio rho of the cells, and finally obtaining the characterization conclusion of the cell mutation degree. The method belongs to the technical field of images, and solves the problem that the cell mutation degree cannot be accurately determined due to the fact that the nucleus-plasma ratio of the cells is difficult to accurately calculate in the prior art.

The utility model provides a kind of floating type's vibration mixing device and cell staining equipment, floating type's vibration mixing device includes slide loading and unloading mechanism, vibration motor, height adjusting mechanism and base, slide loading and unloading mechanism includes slide rack, glass slide and limit post, installation groove is opened on slide rack, glass slide is set in installation groove, slide rack front part is equipped with limit block, glass slide one end abuts limit block, limit post is set in the both sides and rear portion of slide rack, vibration motor is fixedly arranged below slide loading and unloading mechanism, height adjusting mechanism is located between slide loading and unloading mechanism and base.The device simple structure, there is no any shelter above slide, it is convenient for operator to load and unload glass slide.

The present application provides a fluorescent dye, and a preparation method therefor and a use thereof. The fluorescent dye has a structure as shown in formula I, is a silicon-based Rhodamine organic dye, has excellent near-infrared fluorescence characteristics, and contains a conjugated ligand group R in the molecular structure, so that the fluorescent dye can coordinate with various transition metals, thereby improving the response between the metals and the silicon-based Rhodamine dye, and an obtained metal complex can fully exert the characteristics of the complex and the characteristics of silicon-based Rhodamine near-infrared light absorption and emission. The fluorescent dye and the metal complex containing same can be used in the fields of photosensitizers, cell staining, photocatalysis, photodynamic therapy and the like, and have wide application prospects. The preparation method for the fluorescent dye provided by the present application is simple, has mild reaction conditions, is suitable for large-scale preparation, has a high yield and few by-products, is easy to purify to obtain pure products, and greatly widens the application range of silicon-based Rhodamine dyes.

The invention relates to the technical field of biomedical detection, in particular to a multiple myeloma circulating plasma cell flow cytometry method and system, a peripheral blood sample is used for preparing a single-cell suspension, the single-cell suspension is extracted to prepare a to-be-detected tube (TEST tube), the TEST tube is subjected to non-specific site sealing, and the TEST tube is subjected to non-specific site sealing to obtain the multiple myeloma circulating plasma cell flow cytometry method and the multiple myeloma circulating plasma cell flow cytometry system. The method comprises the following steps: taking a TEST tube as a substrate, adding a multi-fluorescent antibody panel containing nano antibodies aiming at BCMA and GPRC5D to perform cell staining operation, performing flow cytometry analysis on the TEST tube to obtain flow cytometry data, and performing gate analysis on the flow cytometry data according to a preset detection analysis strategy to obtain a fluorescence detection result. The number and phenotypic characteristics of circulating plasma cell (CPC) populations are identified and counted, so that circulating plasma cell detection information is generated.

The present invention relates to the technical field of automatic cell staining for medical devices, and particularly relates to an automatic staining module and staining method for formed components of gynecological secretions, including a conveyor belt, a steel needle mounting seat, reagent dropping steel needles, set screws, a reagent dropping cover mounting plate, a reagent dropping cover, a waste liquid sampling needle, a waste liquid sampling needle baffle, a spring, a glass slide, and a glass slide lifting box. The conveyor belt is arranged directly below the reagent dropping cover, and the glass slide lifting box is installed directly below the reagent dropping cover. There are three reagent dropping steel needles, and the three reagent dropping steel needles are installed on the steel needle mounting seat through set screws. The steel needle mounting seat and the reagent dropping cover are installed on the reagent dropping cover mounting plate, and the three reagent dropping steel needles are inserted into the interior of the reagent dropping cover. The waste liquid sampling needle is movably inserted into the steel needle mounting seat and the reagent dropping cover. The protrusion on the waste liquid sampling needle cooperates with the waste liquid sampling needle baffle installed, and the spring is sleeved on the waste liquid sampling needle, making the staining uniform, the process environmentally friendly, and the speed faster.

The invention relates to a light-activated methylene blue preparation process for targeted therapy of various cancers and various hyperplasia cardio-cerebral thrombosis diseases. The death rate of cancer patients is about 80%, heart and cerebral thrombosis severely endangers life, and the occurrence rate of hyperplasia diseases of old people is more than 75%. The key point is that the methylene blue can dye the cells, but after the cells are colored, normal cells can decolor the methylene blue, and cancer cell hyperplasia cells and thrombus cells lose the capability of decoloring the methylene blue. The key point is that methylene blue is a molecule with positive charges, and can be embedded into DNA and RNA of cancer cell hyperplasia cells and thrombus cells after light activation, so that replication is prevented, and targeted therapy is achieved. The traditional Chinese medicine composition is orally taken once every two days by adopting light-activated methylene blue, 120 milligrams of the traditional Chinese medicine composition are orally taken each time, and the traditional Chinese medicine composition is taken for 30-60 times, and has a targeted treatment effect on cancer hyperplasia heart-cerebral thrombosis diseases, and the curative effect is determined by a preparation process. The method for activating methylene blue by light is an effective way for targeted therapy of various cancers and various hyperplasia heart and cerebral thrombosis diseases. The preparation process comprises a preparation process of photo-activated methylene blue and capsule tablet granules.

The invention relates to the technical field of image processing, in particular to an abnormal cell screening method and system based on image analysis, and the method comprises the steps: obtaining continuous image frames of a dyeing process, extracting the average optical density, integral optical density and chromatin gray standard deviation of sample cells as dyeing parameters, and obtaining chromatin textures; the dyeing parameters are changed along with time to form a dyeing parameter space curve, and a continuous dyeing curve is obtained through fitting; aligning and fitting the continuous dyeing curve and a pre-calibrated standard parameter curve to obtain a coordinate transformation vector; and obtaining a dyed image frame, carrying out standardized correction on a to-be-detected cell dyeing parameter by using the coordinate transformation vector to obtain a chromatin texture feature, and carrying out feature comparison to identify an abnormal cell. According to the method, the dyeing sensitivity difference caused by biological factors such as individual gene expression difference, cell membrane protein composition difference and pH value change is reduced, and the influence of dyeing condition difference and equipment difference among different laboratories on screening results is eliminated.

The application discloses a method for simultaneously identifying cell subgroups and related factor phosphorylation levels. The method simplifies the protein phosphorylation flow cytometry staining step without affecting the staining effect, can simultaneously detect 17 stained cells, and simultaneously saves cost and is more efficient. The method provided by the application can provide more accurate distinguishing ability, can obtain more information from a small cell sample, can detect abnormal cells at a low level, increases laboratory efficiency, is especially suitable for the research of the immune system, and has a wide prospect.

The invention discloses a folate receptor mediated cell staining solution and a preparation method and application thereof, and the folate receptor mediated cell staining solution comprises the following components by weight: 0.05%-0.2% of methylene blue, 0.3%-1% of ascorbic acid, 0.3%-1% of folic acid, 1%-3% of acetic acid, 0.1%-0.4% of sodium acetate, 1%-3% of propylene glycol, 0.1%-0.5% of sodium diphenylaminesulfonate, and the balance of water. According to the sodium diphenylaminesulfonate, the cell staining solution can better distinguish different stages of tumors, so that the sensitivity and the accuracy of a detection result are ensured. In addition, the prepared staining solution is long in storage time, the detection time is shortened to a great extent, and the detection mode is simplified to a great extent.

The invention discloses a method for detecting openness of permeability transition pores (MPTP) of peony pollen mitochondria under physiological conditions by computer assistance, which comprises the following steps: preparing Calcein-AM staining working solution and CoCl2 solution, preparing pollen cells to be stained, grouping staining, staining the pollen cells, preparing stained pollen flakes, observing, shooting, and analyzing by computer-aided software (Aivia). And pollen MPTP open fluorescence intensity analysis is carried out. The method has the beneficial effects that the dyeing concentration and steps of Calcein-AM / CoCl2 are optimized aiming at peony pollen cells, the pollen cells and the fluorescence intensity of the pollen cells on each image in different dyeing groups are accurately identified by combining computer-aided analysis, pollen self-luminescence interference is eliminated by taking an undyed group as a reference, and the detection accuracy of the pollen cells is improved. By creating an object classification model based on computer deep learning, fluorescence intensity intervals of MPTP dyeing are accurately divided, and a workflow is created to realize batch processing. According to the method disclosed by the invention, the opening accuracy and visibility of the Calcein-AM / CoCl2 staining method for determining the MPTP are greatly improved, and batch image analysis and processing are realized.

The invention provides a construction method and application of an anti-myocardial damage screening model based on intelligent recognition, and belongs to the field of drug screening models. The mitochondrial active oxygen level is indicated through the mitochondrial superoxide indicator, the mitochondrial morphological parameters are indicated through the mitochondrial dark red fluorescent probe, and the DNA damage level is indicated through Hoechst; the method comprises the following steps: performing high-content imaging after cell staining, analyzing the mitochondrial active oxygen level by adopting a high-content MetaXpress built-in module, analyzing mitochondrial morphological parameters by adopting Image pro plus software, analyzing the DNA foci quantity by adopting a FociNet model to obtain a high-content multichannel image, and further obtaining the spatial representation of a to-be-detected compound through Microsnop image learning. The screening model can accurately distinguish different action mechanisms of compounds, presents specific clusters consistent with phenotypic characteristics in a characteristic space, is high in flux and specificity, and can be used for screening anti-adriamycin myocardial injury positive drugs.

The invention discloses a method and a system for synchronously detecting intracellular microbial colonization and host response at a single cell level, and belongs to the technical field of single cell epimics and microbiomics. Microorganism colonization information and host chromatin openness information of each single cell are synchronously extracted from single cell chromatin accessibility sequencing data, and correlation analysis is performed on the microorganism colonization information and the host chromatin openness information at the single cell level, so that a cell specificity mechanism of host-microorganism interaction is analyzed. At present, a systematic method capable of synchronously realizing microbiological detection and host chromatin accessibility analysis under single cell resolution is not reported yet, and the method fills the technical blank. The method can solve the problem that the existing sequencing technology based on 16S rRNA, 18S rRNA or scRNA-seq is difficult to analyze microorganism colonization and host chromatin accessibility synchronously and highly sensitively at the single cell level, and provides an innovative tool for revealing a host-microorganism interaction mechanism of cell specificity in complex tissues.

The utility model belongs to the technical field of cell staining, and particularly relates to a cell staining device which comprises a working table, the top of the working table is fixedly connected with a supporting frame, and one side of the supporting frame is connected with a first motor. According to the cell dyeing device, the supporting frame, the first motor, the screw rod, the movable block, the flow dividing pipe, the dropper, the connecting pipe, the stirring barrel, the second motor, the stirring rod, the water inlet pipe and the water outlet pipe are matched for use, so that the device can dye a plurality of groups of cells when being used by a worker, and the working efficiency is improved when the worker uses the device. According to the cell staining device, the problems that a staining agent is layered after standing for a long time, a plurality of groups of cells cannot be stained, and the staining agent on the glass slide needs to be manually and uniformly smeared are solved, so that the staining agent is not layered after standing for a long time, and the plurality of groups of cells can be stained; and the staining agent on the glass slide can be automatically and uniformly smeared.

Provided are a single-cell chromatin accessibility and transcriptome joint sequencing method and a use thereof. The method comprises: using a transposase to process a nucleus so as to obtain gDNA of a chromatin open region linked to a first specific tag sequence; using a transcriptome capture sequence to process the nucleus so as to obtain cDNA linked to a second specific tag sequence; generating a droplet on the basis of droplet microfluidics to encapsulate the nucleus and a first microbead in the droplet, wherein the gDNA and the cDNA are captured by the first microbead, and one or more nuclei are encapsulated in the droplet; and sequencing the gDNA and the cDNA, and obtaining single-cell chromatin accessibility and transcriptome joint information on the basis of the first specific tag sequence and the second specific tag sequence.