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Carbon quantum dot fluorescence labeling material with orange peels used as carbon source as well as preparation method and application of carbon quantum dot fluorescence labeling material

A technology of fluorescent labeling and carbon quantum dots, which is applied in the directions of luminescent materials, chemical instruments and methods, etc., to achieve the effects of cheap and easy-to-obtain raw materials, low cost, and simple equipment

Active Publication Date: 2015-04-29
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is simple to synthesize, but requires equipment such as muffle furnaces, microwave ovens, etc. for high temperature and high pressure reactions, and ionic liquids that are difficult to prepare as additives

Method used

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  • Carbon quantum dot fluorescence labeling material with orange peels used as carbon source as well as preparation method and application of carbon quantum dot fluorescence labeling material
  • Carbon quantum dot fluorescence labeling material with orange peels used as carbon source as well as preparation method and application of carbon quantum dot fluorescence labeling material

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Embodiment 1

[0039] A method for preparing a carbon quantum dot fluorescent labeling material using pomelo peel as a carbon source, comprising the following steps:

[0040] (1) Add 0.5g of freshly chopped pomelo peel to 10ml of water, and stir for about 5 minutes;

[0041] (2) Add concentrated H to step (1) 2 SO 4 Solution 3mL, stir for about 5min, until the pomelo peel is carbonized and the solution changes color;

[0042] (3) Add NaOH solution, adjust the pH to 7, and then centrifuge at a speed of 5000r / min for 5min to remove undissolved residues;

[0043] (4) Add 3mL of dichloromethane to the supernatant of step (3), then centrifuge at a speed of 5000r / min for 5min to remove non-fluorescent fat-soluble substances;

[0044] (5) Pass the supernatant in step (4) through a 0.45 μm filter membrane to prepare a fluorescent labeling material.

[0045] From figure 1 It can be seen that the prepared pomelo peel as a carbon source carbon quantum dot fluorescent labeling material is spherical...

Embodiment 2

[0047] A method for preparing a carbon quantum dot fluorescent labeling material using pomelo peel as a carbon source, comprising the following steps:

[0048] (1) Add 0.7g of freshly chopped pomelo peel to 10ml of water, and stir for about 5 minutes;

[0049] (2) Add concentrated H to step (1) 2 SO 4 Solution 3mL, stir for about 5min, until the pomelo peel is carbonized and the solution changes color;

[0050] (3) Add NaOH solution, adjust the pH to 7.5, and then centrifuge at a speed of 5500r / min for 5min to remove undissolved residues;

[0051] (4) Add 1mL of dichloromethane to the supernatant of step (3), then centrifuge at a speed of 5500r / min for 5min to remove non-fluorescent fat-soluble substances;

[0052] (5) Pass the supernatant in step (4) through a 0.1 μm filter membrane to prepare a fluorescent labeling material.

Embodiment 3

[0054] A method for preparing a carbon quantum dot fluorescent labeling material using pomelo peel as a carbon source, comprising the following steps:

[0055] (1) Add 0.3g of freshly chopped pomelo peel to 10ml of water, and stir for about 3 minutes;

[0056] (2) Add concentrated H to step (1) 2 SO 4 Solution 3mL, stir for about 5min, until the pomelo peel is carbonized and the solution changes color;

[0057] (3) Add NaOH solution, adjust the pH to 7, and then centrifuge at a speed of 6000r / min for 5min to remove undissolved residues;

[0058] (4) Add 5 mL of dichloromethane to the supernatant of step (3), then centrifuge at 6000 r / min for 5 min to remove non-fluorescent fat-soluble substances;

[0059] (5) Pass the supernatant in step (4) through a 0.22 μm filter membrane to prepare a fluorescent labeling material.

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Abstract

The invention relates to a carbon quantum dot fluorescence labeling material with orange peels used as a carbon source as well as a preparation method and an application of the carbon quantum dot fluorescence labeling material. The orange peels are used as a raw material, and a simple method for one-step synthesis of a high-fluorescence-quantum-efficiency carbon quantum dot is established. The method comprises steps as follows: the orange peels are mixed with water; then a mixed liquid is mixed with concentrated sulfuric acid; the pH is adjusted by the aid of NaOH, and undissolved residues are removed in a centrifugal manner; then dichloromethane is added to a centrifuged supernate, and a non-fluorescence fat-soluble substance is removed in the centrifugal manner; finally, the centrifuged supernate passes through a film, and the fluorescence labeling material is prepared. According to the method, high-temperature and high-pressure reactions are not required to be performed by equipment such as plasma emitters, hydrothermal reaction kettles, muffle furnaces and the like during synthesis, reaction conditions are mild, operations are simple and convenient, the cost is very low, the method can be implemented in ordinary laboratories, the synthesized fluorescence labeling material lays a foundation for the further application to work such as bio-labeling, heavy metal analysis, antibody labeling, cell staining and the like.

Description

technical field [0001] The invention relates to the technical field of material synthesis, in particular to a carbon quantum dot fluorescent labeling material using citrus peel as a carbon source and a preparation method and application thereof. Background technique [0002] Fluorescent carbon dots, as a new member of the carbon nano family, are dispersed spherical nanoparticles with a size less than 10 nm. They have excellent fluorescence properties such as tunable excitation and emission wavelengths, stable fluorescence, and no light flickering. Compared with traditional semiconductor quantum dots, rare earth fluorescent labeling materials and organic fluorescent dyes, it has the advantages of small particle size and molecular weight, low biotoxicity, and good biocompatibility, as well as adjustable luminous range, good photostability, and no light With the advantages of blinking and easy functionalization, it has great application prospects in the fields of cell imaging a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65
Inventor 范蓓王淼陈卫华王凤忠张晶卢嘉李敏敏戴超贺妍孙玉凤
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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