328 results about "Acid production" patented technology

The invention discloses an ammonia-nitrogen wastewater treatment device and method capable of generating target ammonium salt, the ammonia-nitrogen wastewater treatment device comprises a bipolar membrane electrodialysis device and a membrane contactor, the bipolar membrane electrodialysis device comprises at least one treatment unit and an anode chamber and a cathode chamber which are separated by the treatment unit; the treatment unit comprises a front bipolar membrane, a front cation exchange membrane, a middle bipolar membrane, an anion exchange membrane, a rear cation exchange membrane and a rear bipolar membrane which are sequentially arranged in the direction from the anode chamber to the cathode chamber, and a feeding chamber, an ammonia production chamber, an acid production chamber, a desalting chamber and an alkali production chamber are respectively formed in spaces generated between the corresponding membranes; the membrane contactor comprises a breathable hydrophobic membrane dividing an inner cavity of the membrane contactor into a first chamber and a second chamber, and the first chamber and the second chamber communicate with the ammonia production chamber and the acid production chamber through pipelines correspondingly. And the method has relatively high application and popularization values.

The invention provides an intelligent controller for sulfuric acid production and an intelligent control method for acid making. The method comprises the following steps: 1, establishing an intelligent control model capable of self-identifying environment deviation; 2, evaluating and testing the intelligent control model; the method specifically comprises the steps that a control instruction and a monitoring instruction of production data serve as model input, model output and an actual result are subjected to curve visual comparison, and the accuracy of a model is verified; and 3, switching the successfully tested intelligent control model to a use state, issuing a control strategy formed by a new intelligent control model as an instruction to an actual production control process, and performing acid making control. The invention discloses an intelligent control model capable of self-identifying environment deviation, which not only improves the automation level of an acid production system, but also enhances the capability of coping with external changes and internal fluctuations. The intelligent level and the working efficiency of the control system can be greatly improved.

The invention belongs to the technical field of food fermentation, and provides a lactobacillus plantarum CMRC 1L and application thereof. The lactobacillus plantarum CMRC 1L disclosed by the invention is separated from a sour meat sample in Maojia village region in Lijiang City, Yunnan Province, grows well on an MRS culture medium, is high in acid production speed, has the biological characteristics of salt resistance, frostbite resistance, inhibition of growth and reproduction of spoilage microorganisms and the like, has a good fermentation effect on various plant proteins, and is an excellent strain for preparing a leavening agent for production. The invention also provides a leavening agent containing the strain and application of the leavening agent in vegetable protein meat products. Compared with the vegetable protein jerky without the leavening agent, the vegetable protein jerky product prepared by the preparation method provided by the invention has the advantages of stable quality, fresh and sweet fermentation fragrance, no beany flavor and good flavor and taste. The strain CMRC 1L has great potential when being applied to production of vegetable protein meat products.

The invention belongs to the technical field of flue gas acid making, and particularly discloses an acid making fan outlet pressure soft measurement method based on LSTM (Long Short Term Memory), which comprises the following steps of: performing cleaning, down-sampling and normalization processing on fan inlet and outlet pressure original data; based on a time sequence analysis technology, extracting input features in the preprocessed data; an LSTM network is adopted to establish a soft measurement model, and long-term dependence characteristics in a time sequence are captured through a gating mechanism; performing training optimization on model hyper-parameters through grid search; and the model evaluates and predicts the performance by using the data of the test set, deploys the qualified model to an industrial control system, and predicts, regulates and controls the outlet pressure of the acid making fan in real time. According to the method, soft measurement and LSTM are combined, long-term dependency and dynamic change information in a time sequence are extracted, weighting processing is carried out on different scale features to improve prediction precision, real-time prediction of multi-step variables is achieved, and the method has the advantages of being high in prediction precision, high in timeliness and low in hardware cost.

The invention discloses a Weissella sinus strain for improving the fermentation flavor of American ginseng and application of the Weissella sinus strain. According to the present invention, through the comprehensive evaluation of a variety of lactic acid bacteria, including the acid production capacity, the chroma regulation and the flavor characteristic analysis, the optimal strain for the American ginseng fermentation is finally screened, and the strain shows the significant flavor regulation advantage. On one hand, characteristic earthy smell substances of American ginseng can be completely degraded, and the bad flavor of the raw materials is effectively improved; on the other hand, a compound with a fruity flavor characteristic can be specifically generated, and a fermentation product is endowed with a remarkable fragrant and sweet flavor. In addition, the strain disclosed by the invention maintains proper acid production capacity and chroma stability, and the flavor quality is remarkably improved on the premise of ensuring the fermentation efficiency. Due to the characteristics, the strain becomes an ideal fermentation strain for developing a high-quality fermented American ginseng product.

The invention relates to the technical field of sewage treatment, in particular to a multistage fermentation acid production preposed phosphorus recovery device which comprises an aerated grit chamber, a fermentation primary sedimentation tank, a preposed sequencing batch type phosphorus removal system, a treatment and discharge system and a recovery system, the aerated grit chamber is connected with an external sewage pipeline, the fermentation primary sedimentation tank is arranged on one side of the aerated grit chamber, and the preposed sequencing batch type phosphorus removal system is arranged on the other side of the aerated grit chamber. The front sequencing batch dephosphorization system is communicated with the fermentation primary sedimentation tank, and the treatment and discharge system is communicated with one side of the front sequencing batch dephosphorization system. Compared with the prior art, by arranging the fermentation primary sedimentation tank and reasonably controlling the hydraulic retention time and the sludge concentration, not only are fermentation conditions in the tank optimized, but also unnecessary stirring energy consumption and excessive sludge treatment requirements are reduced, the operation cost is reduced, and meanwhile, the sludge load of a subsequent system is effectively reduced.

The invention relates to the field of sludge circulation treatment, in particular to a sludge circulation treatment method capable of reducing pollution and carbon. The sludge circulation treatment method capable of reducing pollution and carbon comprises the following treatment steps: S1, sludge disintegration: selecting a sludge sample in sewage, and screening out dominant bacteria and reproduction conditions; s2, anaerobic digestion treatment: adding the screened strains into a sludge anaerobic digestion device for anaerobic digestion treatment; s3, sewage treatment: conveying the sewage subjected to anaerobic digestion treatment into a sewage treatment device, and selecting organic matters dissolved out of sludge in the step of anaerobic digestion treatment as a denitrification carbon source for biological treatment. According to the present invention, the sludge digestion innovative process is adopted, the original anaerobic reaction process in the sewage process is utilized, the decomposition and acidification effects on the biochemical excess sludge are enhanced, the methanogenesis reaction is inhibited through the internal ammonia nitrogen, the dissolution acid production efficiency is improved, the reaction time is shortened, and the on-site resource utilization of the sludge is achieved.

The invention relates to the technical field of bio-organic fertilizer fermentation monitoring, in particular to a bacillus bio-organic fertilizer fermentation condition monitoring and optimizing method. The method comprises the steps of obtaining a data change degree characterization value and a related difference characterization value of a first detection point, obtaining a target bacillus acid production characterization value of the first detection point according to the data change degree characterization value and the related difference characterization value of the first detection point, and obtaining a target bacillus acid production characterization value of the second detection point according to the target bacillus acid production characterization value. Screening the first detection point again, obtaining a predicted PH value at a future monitoring moment according to a screening result, and monitoring the fermentation process of the bacillus bio-organic fertilizer according to the predicted PH value at the future monitoring moment. And the timeliness or timeliness of monitoring the pH value of the fermentation liquid in the fermentation tank can be improved by predicting the pH value.

The present invention provides a method for increasing the fermentation yield of L-lysine, and belongs to the field of L-amino acid fermentation. The method for increasing the fermentation yield of L-lysine is composed of the following steps: preparing a shake flask culture solution, preparing a seed solution, and fermenting and culturing. The method for increasing the fermentation yield of L-lysine of the present invention can effectively improve the tolerance of the bacterial body to high osmotic pressure, improve the acid production performance in the late fermentation period, and improve the single-tank yield and sugar-acid conversion rate of L-lysine; at the same time, further reduce the adverse effects of osmotic pressure regulators on the fermentation stability of L-lysine, and effectively avoid the problem of excessive addition of osmotic pressure regulators inhibiting bacterial metabolism, extending the fermentation production cycle, and reducing fermentation efficiency.

The invention provides a bean product fermentation influence factor analysis method and system, and relates to the technical field of food safety detection.The method comprises the steps that multiple sets of key parameters are set within a preset range, physical and chemical index changes under the parameters are monitored in real time, and the physical and chemical index changes comprise the tannin variation, the acid production capacity, the fermentation rate and the active enzyme variation; the method comprises the following steps: establishing a key parameter, constructing a physical and chemical index prediction model, training by taking the key parameter as input, evaluating a fermented bean product, training a sensory evaluation index prediction model by taking the physical and chemical index as input, and finally, determining an optimal combination of the key parameter through a genetic algorithm by taking maximization of the sensory evaluation index as a target, thereby optimizing a fermentation process. By setting and adjusting key fermentation parameters in real time and combining physical and chemical index monitoring and sensory evaluation, the purposes of optimizing the fermentation process and improving the final quality of bean products are achieved.

The utility model discloses a photoelectrochemistry-based organic matter-rich wastewater hydrolysis device which comprises a hydrolysis acid production tank, a water inlet and a gas outlet are arranged on the hydrolysis acid production tank, the water inlet is connected with a water inlet pipe, the water inlet pipe is connected with a reservoir, a water delivery pump is arranged in the reservoir, the gas outlet is connected with a gas outlet pipe, and the gas outlet pipe is connected with a gas collection tank; a photoelectrochemical integrated device for accelerating the hydrolysis acid production process is arranged on the inner wall of the hydrolysis acid production tank, and a stirring device is arranged in the hydrolysis acid production tank; the hydrolysis acid production tank is provided with a main feed port and an auxiliary feed port, the bottom of the hydrolysis acid production tank is provided with a liquid outlet and a slag outlet, the liquid outlet is connected with a liquid outlet pipe, and the slag outlet is connected with a slag outlet pipe. The method is convenient, fast and high in feasibility, proliferation and metabolism of microorganisms are promoted through the photoelectrochemical integrated device, the reaction speed can be greatly increased, then the treatment effect and treatment efficiency of the wastewater are integrally improved, and the technical problems that existing organic matter-rich wastewater is poor in treatment effect and low in treatment efficiency are effectively solved.

The invention discloses a method and a system for efficiently producing short-chain fatty acid by utilizing waste molasses in the sugar industry and municipal sludge through synergistic anaerobic fermentation, and belongs to the technical field of organic solid waste recycling. Waste molasses and municipal sludge are mixed according to a specific ratio, the carbon-nitrogen ratio is optimized to 20-30: 1, trace metal catalysts (Fe < 2 + >, Co < 2 + > and Ni < 2 + >) are added, and two-stage pH regulation (the initial pH is 6.0-7.0, and the later pH is 5.5-6.0) and a medium-temperature (35-37 DEG C) anaerobic fermentation process are combined, so that the yield of short-chain fatty acid is remarkably increased to 5.2-6.8 g COD / L and is increased by 200-300% compared with that of traditional sludge single-substrate fermentation. The method realizes recycling and high-value conversion of the organic solid waste, and has the characteristics of low cost, high acid production efficiency and environmental friendliness.

The invention discloses Pediococcus pentosaceus G41, which is separated from rumen fluid of healthy adult dairy cows in a certain cattle farm in Changchun, has the advantages of high growth speed and strong acid production capacity, and can effectively relieve cholestatic liver injury. Experiments prove that the Pediococcus pentosaceus G41 reduces the activity of serum AST, ALT and GDH of a mouse with cholestatic liver injury, reduces the content of total bile acid in blood and liver, increases the content of total bile acid in excrement, and improves cholestatic and dirty injury caused by cholestatic.

The invention discloses a method for producing L-arginine through escherichia coli fermentation. The escherichia coli has the characteristics of high L-arginine yield and low heteroacid content. In the fermentation process, fed-batch sugar and sodium glutamate are adopted according to the thallus concentration OD600 and the fermentation period; the concentration of glutamic acid is controlled by feeding sodium glutamate or glutamic acid precursor sodium succinate and sodium citrate, the arginine acid production and sugar acid conversion rate is increased, the content of heteroacid is reduced, the final acid production can reach 13.9% at most, the sugar acid ratio is 57.2%, and the heteroacid content is not higher than 0.3%. The process solves the control difficulty in the industrial process of arginine, and has great commercial value.

The present invention provides a method for preparing a keratinized gingival repair scaffold, wherein the active powder of biological microspheres is dispersed in a protein slurry to obtain a loose surface stock solution, which is freeze-dried together with a cell-free matrix material, and after two cross-linkings, a keratinized gingival repair scaffold with a loose surface and a dense surface is obtained. When in use, the loose surface of the porous sponge structure contacts the wound surface, which is conducive to the attachment and growth of tissue cells, and the dense surface can seal and protect the wound to a certain extent in an open healing environment. The obtained keratinized gingival repair scaffold can effectively maintain and regulate the pH environment in the oral cavity, inhibit bacteria and resist bacteria, and inhibit the growth of acid-producing bacteria such as Streptococcus mutans and bacteria with strong caries-causing properties, and at the same time has the ability to stop bleeding, promote bone growth and periodontal ligament regeneration, and inhibit periodontal epithelial regression.

The invention discloses a dry-type high-pressure anaerobic fermentation system and method for agricultural waste, and belongs to the technical field of biogas fermention.The dry-type high-pressure anaerobic fermentation system comprises a mixing pool, a feeding tank and an anaerobic fermentation reactor, and the feeding tank is connected with a hydrolysis pool feeding pipeline and an acid production pool feeding pipeline; the anaerobic fermentation reactor comprises a hydrolysis tank, an acid production tank and a methane production tank which are separated by a reactor outer wall, a first separation wall and a second separation wall which are arranged in a concentric circle, and a biogas collection area formed by a separation top plate and a reactor dome is arranged at the top end of the reactor outer wall; an overflow channel is formed at the top end of the first separating wall; a plurality of separating wall supporting columns are circumferentially and uniformly distributed at the bottom of the second separating wall; a leachate tank is correspondingly arranged at the bottom end of the methanogenesis tank, and a plurality of biogas slurry lifting pipes are arranged in the methanogenesis tank; a fermentation waste discharge pipeline is arranged on the side wall of the second separation wall, a biogas discharge pipe is arranged on the dome of the reactor, and the fermentation system is stable in operation, small in consumption of circulating inoculum and high in volume gas production rate.

The invention relates to the technical field of cool acid production, and discloses a cool acid production heat exchanger based on a transverse tube falling film evaporation technology, which comprises a tank body, a connecting ring a fixedly sleeved on the tank body, a connecting ring b fixedly connected on the connecting ring a through a bolt and a nut, a tank tail fixedly connected in the connecting ring b, and two support frames fixedly mounted on the tank tail, a fixing block is arranged in the tank tail, a plurality of arc-shaped grooves are formed in the fixing block, an arc-shaped pipe is movably connected into each arc-shaped groove, each connecting pipe is connected to a heat exchange pipe in the tank body in a sleeving mode, then the tank body and the tank tail can be fixed through bolts and nuts, and therefore the arc-shaped pipes can be connected with the heat exchange pipes. And when the heat exchange tubes need to be cleaned, the tank tail can be detached, so that the heat exchange tubes and the arc-shaped tubes can be cleaned one by one, and the situation that the arc-shaped parts of the arc-shaped heat exchange tubes are difficult to clean can be avoided.

The invention discloses a fermentation production process for improving the conversion rate of L-arginine and a strain used by the fermentation production process, and belongs to the technical field of fermentation engineering. According to the method, the content of residual sugar and ammonia nitrogen in the fermentation process of the L-arginine is accurately regulated and controlled, so that the growth speed of thalli and the sugar-acid conversion capacity are controlled, the fermentation period is shortened, meanwhile, the fermentation period of the L-arginine is 44-55 hours, the produced acid reaches 130 g / L or above, and the conversion rate is 50% or above by supplementing materials in the fermentation process and controlling other processes. According to the process, the acid production and conversion rate of the strain are greatly improved, and the production cost is reduced.

The invention provides fermented lactobacillus mucus BL-SWN1 and application thereof, the fermented lactobacillus mucus BL-SWN1 is screened from naturally fermented soybean clear water, is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 6, 2025, is named as Limosilicilla fermentum in taxonomy, has a preservation number of CGMCC No.33307, and belongs to the technical field of microorganisms. The lactobacillus mucus BL-SWN1 has good acid production capacity, gastrointestinal fluid and cholate tolerance, bacteriostatic activity and nitrite degradation capacity, the pH value of a culture medium can be reduced to about 4.3 within 24 h of culture, and the nitrite degradation rate reaches 95% or above. The fermentation lactobacillus mucus BL-SWN1 can be applied to vegetable fermentation products such as pickled Chinese cabbages and pickled vegetables, can also be suitable for the fermentation process of dairy products and meat products, has a wide application prospect in the food industry, and particularly has remarkable advantages in the aspect of development of healthy fermentation foods.

The invention provides a coagulation impurity filter for 2-chloronicotinic acid production sewage treatment, the coagulation impurity filter comprises a sedimentation tank, a shell and an outer shell, a transverse plate, a first partition plate and a second partition plate are fixedly mounted in the sedimentation tank, a flow guide mechanism is rotatably connected in the sedimentation tank, the shell and the outer shell are both fixedly connected with the outer wall of the sedimentation tank, and the first partition plate and the second partition plate are arranged in the shell. A cleaning mechanism is arranged in the shell, a deslagging mechanism is arranged at the bottom of the sedimentation tank, a blocking mechanism is arranged in the shell, a sweeping mechanism is arranged at the bottom of the screen frame, and the interior of the shell communicates with the interior of the sedimentation tank through a water pumping mechanism. According to the sewage treatment device, the sedimentation tank is adopted for conveying sewage, rapid discharge of impurities is achieved through back-and-forth movement of the push plate, multi-stage filtration can be conducted on the sewage with the impurities precipitated through the multiple screen frames, real-time sweeping is achieved while the sewage is conveyed, and the sewage can be quickly discharged through opening of the plugging mechanism and starting of the water pumping mechanism. Impurities collected at the filter screen can be treated, and real-time treatment can be carried out to ensure high efficiency and stability of the filter in working.

The invention provides a segmented direct vat set chilli sauce composite leavening agent and a method for preparing chilli sauce by using the same, and belongs to the technical field of food fermentation. The composite leavening agent comprises a leavening agent 1 and a leavening agent 2, wherein the leavening agent 1 is prepared from freeze-dried powder of plant lactobacillus CICC 25125, freeze-dried powder of lactobacillus acidophilus CICC 6094 and freeze-dried powder of leuconostoc mesenteroides CICC 24385, and the leavening agent 2 is prepared from freeze-dried powder of saccharomyces cerevisiae, a fermentation accelerant and garlic essential oil. The composite leavening agent can effectively control the fermentation process, perform segmented intensified inoculation, increase the acid production rate, shorten the fermentation period and reduce the generation of nitrite in the fermentation process. The invention further provides a method for preparing the chilli sauce by using the composite leavening agent, and the prepared chilli sauce is low in nitrite content, presents soft and rich fruity flavor and is high in flavor.

The utility model discloses a system for realizing online circulating flow and fermentation of amino acid by adopting a ceramic membrane, which comprises a strain tank, a seed tank arranged on one side of the strain tank, a fermentation tank arranged on one side of the seed tank, a feed pump arranged at the top end of one side of the fermentation tank, a cleaning tank arranged on one side of the fermentation tank, and a circulating pump arranged at the bottom end of one side of the cleaning tank. According to the amino acid on-line circulating flow and fermentation system realized by adopting the ceramic membrane, the culture times of early-stage strain culture and seed tank culture are obviously reduced; the fermentation level is always in a high level state, the acid production rate of thalli is high, the yield is remarkably improved under the condition that equipment is not increased, and the total yield even can reach 2-3 times of the original yield; nutrient substances in the ceramic membrane filtrate can be sterilized after being extracted to serve as nutrient substances to be fed into the fermentation tank, so that the product output ratio of feeding is increased, namely the acid yield is increased; and the ceramic membrane can be sterilized by steam, so that the requirement of sterile equipment is met.

The invention discloses a microorganism, a microbial agent, food, a method for preparing the food and a method for producing pectinase, and relates to the field of microorganisms. The microorganism is plant lactobacillus, and the preservation number of the microorganism is CGMCC (China General Microbiological Culture Collection Center) No.31283. The new strain of the plant lactobacillus obtained through screening has the advantages of being high in growth speed, high in acid production capacity, acid resistance and salt resistance, high in pectinase production capacity, high in nitrite and nitrate metabolism capacity and the like; the new strain is used for developing salt-reduced fermented food, the process adaptability is good, and the prepared fermented food is rich in lactic acid bacteria content, nutritional, healthy and unique in flavor; a food fermentation process developed by utilizing the new strain is high in fermentation efficiency, short in production period and high in application value.

The invention relates to the technical field of chemical equipment, and provides a tetrazoleacetic acid production system which comprises a glycine tank, a triethanol orthoformate tank, a glacial acetic acid tank, a regulating liquid tank, a pretreatment kettle, a refining reaction kettle and a nitrogen displacement device, a feeding mechanism for feeding sodium azide is arranged at the upper end of the refining reaction kettle, and a nitrogen replacement pipe of the nitrogen replacement device can be alternatively communicated with the feeding mechanism or the refining reaction kettle; when the sodium azide is fed, the nitrogen replacement pipe is communicated with the feeding mechanism, and the feeding mechanism can enable the sodium azide to enter the refining reaction kettle under the pushing of nitrogen; according to the invention, the feeding of sodium azide can be combined with the nitrogen replacement device through the feeding mechanism, the feeding work of sodium azide is driven by nitrogen, manual feeding is eliminated in the production system, and the convenience and safety are improved.

The invention discloses a sludge anaerobic acidogenesis strengthening method based on pyrohydrolysis and potassium permanganate synergistic degradation of melanoids. The method comprises the following steps: (1) screening sludge to remove impurities, and then carrying out gravity settling concentration; (2) pyrohydrolysis treatment: high-temperature and high-pressure treatment, pressure relief and cooling; (3) adding potassium permanganate for oxidation, and performing ultrasonic dispersion; (4) anaerobic fermentation: inoculating activated sludge, and controlling fermentation conditions; and (5) separating and extracting the short-chain fatty acid. Through the synergistic effect of pyrohydrolysis and potassium permanganate, melanoids generated in the pyrohydrolysis process are degraded in a targeted mode, the inhibition effect of melanoids on anaerobic microorganisms is eliminated, and meanwhile efficient dissolution of organic matter in sludge is promoted. Nano-manganese dioxide generated by oxidizing potassium permanganate serves as an electron transfer medium, the metabolic activity of acid-producing bacteria is further enhanced, and the acid pickling recovery rate is larger than or equal to 90%. According to the method, the yield of short-chain fatty acid is increased by 65% (as high as 456mg / g VS), high efficiency and environmental friendliness are achieved, and an innovative solution is provided for sludge resourceful treatment.

The invention provides a sulbactam acid production process, sulbactam acid is prepared from a raw material 6-APA through the steps of bromination, water washing separation, oxidation, decoloration, cooling centrifugation, reduction and the like. Compared with a traditional synthesis mode of adding an oxidant solution, the method has the advantages that the usage amount of a solvent and water is effectively reduced, so that the generation amount of wastewater after reaction is reduced, the production of production wastewater can be effectively reduced, and the wastewater treatment cost can be saved. In addition, the oxidation process of the process is completed in a water phase, and the use of an organic solvent is reduced, so that the process has the characteristics of production cost saving, low pollution in the reaction process, easiness in operation in the reaction process and mild reaction conditions.

The invention provides a preparation-free authigenic composite acid fracturing fluid and application thereof. The preparation-free authigenic composite acid fracturing fluid comprises an acid generator, an acid generator catalyst and a preparation-free fracturing fluid. The preparation-free authigenic composite acid fracturing fluid not only has the performance of a fracturing fluid, but also has the performance of an acid fluid. The method has the advantages that few additives are adopted, an acid generator is single, products obtained after hydrolysis under certain conditions are not only hydrochloric acid or organic acid, but hydrochloric acid and organic acid are adopted, and acid-rock reaction is more facilitated; acid production products are controllable; the acid generation temperature is high, and acid generation can be carried out under the condition of 150-200 DEG C; the effective efficiency is high, and the H < + > concentration is up to 1.36-4.6 mol / L. The fracturing fluid system can realize on-line preparation-free, simplify the on-site liquid preparation process, change the fracturing fluid formula at any time according to the requirement of a reservoir for liquid, achieve the effect of volume transformation of the fracturing fluid and the effect of acidification at the same time, and achieve the purpose of uniform acid distribution by enabling acid generated at the reservoir temperature to reach the top end of a crack along with the fracturing fluid.

This invention relates to the field of microbial technology, and particularly to a compound lactic acid bacteria fermentation agent and its application, a method for producing *Anoectochilus roxburghii* fermentation broth, and related products. The compound lactic acid bacteria fermentation agent comprises *Lactobacillus plantarum* BXM2, *Lactobacillus fermentatus* J IAN, and *Lactobacillus paracasei* YYS-69. Fermenting *Anoectochilus roxburghii* broth using this compound fermentation agent results in stronger acid production, higher viable bacterial counts, and significantly improved antioxidant activity, total polyphenol and flavonoid content, and enhanced hypoglycemic activity. Sensory effects are also significantly improved. This compound lactic acid bacteria fermentation agent, when applied to the fermentation of traditional Chinese medicine, can enhance the antioxidant capacity and effective functional component content of the fermentation broth, thereby improving efficacy. It has great application prospects in the field of deep-processed *Anoectochilus roxburghii* products and even other fermented medicinal and edible products.

The invention discloses an automatic control method and system for fumaric acid production. The method comprises the following steps: collecting process variables in a fermentation process; constructing a soft measurement model fusing physical modeling and a residual neural network to obtain an acid production rate prediction value and prediction confidence; calculating a feed-forward control quantity, and adjusting the weight according to the prediction confidence to obtain a feed-forward control output; constructing a state vector, and establishing a Markov decision process; training a neural network control strategy by adopting a near-end strategy optimization algorithm, and outputting a proportionality coefficient, an integral coefficient and a differential coefficient; calculating feedback control quantity, fusing the feedback control quantity with feed-forward control output, and applying change rate and amplitude limitation to obtain final control output; and switching to a feedback priority control mode under a specific condition, and regularly updating a soft measurement model and a control strategy parameter. According to the invention, high-precision stable control of pH in the fumaric acid fermentation process is realized, the production efficiency and the process robustness are improved, and the method is suitable for automatic control of the large-scale fermentation process.

The invention relates to a method for preparing fish feed by secondary utilization of traditional Chinese medicine wastes, and belongs to the technical field of fish feed. After the traditional Chinese medicine waste is pretreated to remove heavy metal, primary fermentation of bacillus subtilis and aspergillus niger is carried out, the bacillus subtilis can degrade cellulose, the aspergillus niger decomposes antinutritional factors, then fermentation of lactobacillus lactis and candida utilis is carried out, lactic acid bacteria produce acid to inhibit infectious microbes, flavor substances are synthesized, and palatability is improved. Through traditional Chinese medicine residue pretreatment, multi-strain synergistic fermentation and a scientific feed formula, efficient reutilization of traditional Chinese medicine waste is successfully realized, and the fish feed which is rich in nutrition, high in safety and remarkable in economic benefit is produced.