210 results about "Synechococcus" patented technology

The invention relates to aerobic denitrifying paracoccus denitrificans and application thereof. A bacterial strain provided in the invention is paracoccus denitrificans DN-3CGMCC No. 3658; the Paracoccus denitrificans can carry out aerobic denitrification by using nitrate nitrogen under both aerobic and oxygen-limited conditions and can also carry out heterotrophic nitrification-aerobic denitrification by using ammonia-N, and a total nitrogen removal rate on above mentioned occasions is greater than 90%. The paracoccus denitrificans provided in the invention has stable hereditary features, isapplicable to treatment of a variety of nitrogen-containing waste water and produces a good nitrogen removal effect.

The invention belongs to the technical field of veterinary microbial additive preparation, and specifically relates to a strain of separated and screened Enterococcus faecium providing significant bacterial inhibition effects for common enteropathogenic bacteria such as staphylococcus aureus, escherichia coli and salmonella in breeding animals, and an application thereof. The probiotic Enterococcus faecium of the present invention is characterized in that: the strain is Enterococcus faecium HDRsEf1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CCTCC NO:M2011031. The probiotic Enterococcus faecium of the present invention has characteristics of fast growth, high acid production capacity, strong stress resistance, safety, disease resistance and growth promotion, and can be used as the microbial feed additive for livestock and poultry feeds.

The invention discloses a pyridine-degrading bacterium as well as a composite bacterial agent, a preparation method and uses thereof. The newly-separated Rhodococcus pyridinovora KT-J002 CGMCC No.2789 and Cellulomonas sp KT-J007 CGMCC No.2788 not only can effectually degrade the high concentration pyridine, but also has tolerance or degradation ability for toxic substances such as benzene, phenol, dimethylbenzene, quinoline, cyanide and the like; the composite bacterial agent composed of Rhodococcus pyridinovora KT-J002 CGMCC No.2789, Cellulomonas sp KT-J007 CGMCC No.2788, Paracoccus d enitrificans W12 CGMCC No.1673, Micrococcus luteus ATCC 49442 and Arthrobacter crystallopoietes ATCC 15481 is used for environmental improvement, can effectually degrade the pyridine and is particularly suitable for the biological treatment of coking waste water.

Disclosed herein is a method for treating odors in wastewater treatment bodies. The pH of the body is preferably established above about 6 and most preferably above about 7, usually by adding a base to the body. The oxidation reduction potential of the body is established at about −300 mV or higher and more preferably about −200 mV or higher, usually by adding an oxidizing agent such as oxygen or nitrates. After these conditions are established, sulfur oxidizing bacteria are added to the body. These preferably include Paracoccus denitrificans (formerly called Thiosphaera pantotropha) ATTC number 35,512. The sulfur oxidizing bacterial population should be established at levels of at least about 1×104 cfu / mL and more preferably at levels of 1×105 to 1×106 cfu / mL or higher. The increased ORP and pH levels make sulfate reduction thermodynamically unfavorable. Additionally, the presence of an alternative electron acceptor such as oxygen or nitrate allows the sulfur oxidizing bacteria to oxidize organic acids.

The invention discloses a denitrogenation paracoccus strain and the cultivating method and the application. The strain is Paracoccus denitrificans W12 CGMCC No.1673. It has the strong degradation ability to organic pollutant, and could be used to take the biology treatment to the polluted water containing benzene, xylene, quinoline and cyanide and to the polluted soil. Especially, it has high degradation activity to benzene.

This invention discloses Pediococcus acidilactici LH31 (CCTCC M207013), and a method for producing pediocin from it. The method comprises: inoculating Pediococcus acidilactici LH31 in modified MRS liquid medium, culturing, activating, inoculating activated Pediococcus acidilactici LH31 in a fermentation tank, and performing feed-batch fermentation with three stages. Pediococcus acidilactici LH31 is proliferated in stages 1 and 2. The maximal bacterial density (OD600 nm) can reach 30. Pediocin is accumulated in stages 2 and 3. The maximal titer can reach 18000 AU / mL. The method is suitable for industrialization.

The invention provides a sauerkraut (Direct Vat Set) DVS. Five lactobacillus production leavens such as lactobacillus plantarum, pediococcus acidilactici, leuconostoc mesenteroides, lactic lactococcus, lactobacillus brevis, and the like, are firstly prepared; the viable count of all production leavens is not less than 10<9> / ml; subsequently, the five lactobacillus production leavens are frozen dryly; according to the weight portions of the dryly frozen bacteria powder, 3-5 of lactobacillus plantarum, 2-4 of pediococcus acidilactici, 1-3 of leuconostoc mesenteroides, 1-3 of lactic lactococcus and 3-5 of lactobacillus brevis are taken and uniformly mixed so as to obtain the mixed dryly frozen leaven powder. The product of the invention can obviously shorten the fermentation period of the sauerkraut, lead the product to have acid bitterness and good taste, greatly reduce the content of the nitrite in the product, improve the edible safety of the product, and lead the yield of the sauerkraut to be improved from about 30% of natural fermentation to more than 40% at the same time.

This disclosure relates to modified Streptococcus pneumonia pneumolysm (PLY) proteins which lack hemolytic activity and can be used as immunogens in an immunogenic composition or vaccine against invasive pneumococcol diseases caused by S. pneumonia. The modified pneumolysm proteins comprise ammo acid substitutions at threonine 65, glycine 293 and cysteine 428 Nucleic acids, polypeptides encoded thereby, compositions containing the same, methods for using such nucleic acids, polypeptides and compositions are also provided

The invention discloses paracoccus and the application for degrading azotic heterocyclic compound. The paracoccus is paracoccus sp. BW001 CGMCC No. 2225. The paracoccus sp. BW001 CGMCC No. 2225 of the invention, on one hand, can grow into pyridine as the only carbon source and nitrogen source, which has a strong degradation activity (the degradation rate is 100%) for pyridine, is extremely suitable for biologic control of organic wastewater (such as coking wastewater) which contains pollutant of pyridine category, and can be used as biological hardening agent in the biological control technology of organic persistent wastewater, and corresponding environmental biological agent is exploited, thereby having relatively high value of study, application and marketing.

The invention discloses a pediococcus pentosaceus strain, ferment produced thereby and the use of ferment in meat product, wherein the docket number of the R9(Pediiococcus Pentosaceus I9) strain in the Chinese Microbiological Culture Preservation Administration Commission Common Microbiological Center is CGMCC No.0918. The leaven prepared from the bacterial can be applied into the fermentation production of meat food.

The invention relates to a salt-tolerant COD removal denitrifying microbial agent. The microbial agent consists of at least one of paracoccus sp FSTB-2, microbacterium kitamiense FSTB-4 and pseudomonas stutzeri FSTB-5, and further consists of at least one of paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3, wherein the paracoccus sp FSTB-2, the microbacterium kitamiense FSTB-4 and the pseudomonas stutzeri FSTB-5 are preserved in China General Microbiological Culture Collection Center on June 1, 2015, with preservation numbers of CGMCC No.10938, CGMCC No.10939 and CGMCC No.10940; and the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are disclosed in CN102465104A and CN102465103, and the preservation numbers of the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are CGMCC No.3658 and CGMCC No.3660. The microbial agent can be used for directly processing total nitrogen and COD in high-salinity wastewater, and the microbial agent can be also added to various biochemical reaction constructions so as to improve microbiological composition, optimize the salt tolerance of a microbial system in wastewater treatment and improve total nitrogen and COD removal rate of the entire process.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

Disclosed herein is a method for treating odors in wastewater treatment bodies. The pH of the body is preferably established above about 6 and most preferably above about 7, usually by adding a base to the body. The oxidation reduction potential of the body is established at about -300 mV or higher and more preferably about -200 mV or higher, usually by adding an oxidizing agent such as oxygen or nitrates. After these conditions are established, sulfur oxidizing bacteria are added to the body. These preferably include Paracoccus denitrificans (formerly called Thiosphaera pantotropha) ATTC number 35,512. The sulfur oxidizing bacterial population should be established at levels of at least about 1x10<4 >cfu / mL and more preferably at levels of 1x10<5 >to 1x10<6 >cfu / mL or higher. The increased ORP and pH levels make sulfate reduction thermodynamically unfavorable. Additionally, the presence of an alternative electron acceptor such as oxygen or nitrate allows the sulfur oxidizing bacteria to oxidize organic acids.

The invention relates to a cultural method for paracoccus denitrificans and application of the same to purifying aquaculture water, and belongs to the technical field of aquaculture. Various low-value materials such as molasses and waste liquid of monosodium glutamate and production waste which are harmless for aquaculture organisms and the paracoccus denitrificans are used as raw materials, the raw materials are reasonably compounded into liquid culture media, paracoccus denitrifican liquid with high activity and high concentration is produced, the liquid is directly fed into the aquaculture water, an ecological environment of the aquaculture water is repaired, and an excellent water environment beneficial to survival and growth is created for the aquaculture organisms. The culture method and the application have the advantages that the paracoccus denitrificans become effective fast after being used, an inorganic nitrogen removal form and the removal speed can be selected according to an oxygen dissolving condition of the water, contents of harmful substances such as ammonia nitrogen, nitrite and nitrate in the water are remarkably reduced, the quality of the water environment and a survival condition for the aquaculture organisms are improved, the yield of the aquaculture organisms is increased, and the quality of the aquaculture organisms is improved.

The invention belongs to the field of applied microbiology, and relates to Corallococcus coralloides and application thereof. The Corallococcus coralloides EGB is preserved in China Center for Type Culture Collection (CCTCC), wherein the preservation data is 17th, December, 2012 and the preservation number is CCTCC NO: M2012528. In the growing process of the strain, the strain has good inhibiting effect to growth of plant pathogenic fungi of paddy false smut, fusarium wilt of cotton and fusarium wilt of cucumber. The fermented supernate of the strain has remarkable splitting action to hypha of pathogenic fungi. Meanwhile, abundant secondary metabolites generated by the strain have potential research value.

The invention discloses a domestic sewage treatment microbial inoculant which comprises polyacrylamide, polyethyleneimine, silicon dioxide and compound microorganisms, wherein the compound microorganisms include Bacillus subtilis, Pseudomonas aeruginosa, Bacillus cereus, Candida lipolytica, nitrobacteria and Paracoccus denitrificans. The treatment agent can effectively treat domestic sewage, is low in cost and favorable in effect, and reduces pollution of the domestic sewage to the ecological environment; the sewage suspended substance removal rate is up to 99.8%, the BOD (biochemical oxygen demand) removal rate is 98%, the COD (chemical oxygen demand) removal rate is 98.6%, the chroma is 5, and the sludge quantity is so low that no sludge treatment system needs to be arranged; and the domestic sewage treatment microbial inoculant causes no secondary pollution, and is stable in treatment effect, environment-friendly and non-toxic.

The invention discloses an industrial sewage treatment agent, comprising polyacrylamide, silicon dioxide, peroxysulphate, sodium carbonate and compound microorganisms, wherein the compound microorganisms comprise bacillus licheniformis, pseudomonas aeruginosa, bacillus cereus, candida lipolytica, nitrifying bacteria and paracoccus denitrificans. By adopting the industrial sewage treatment agent disclosed by the invention, the removal rate of sewage suspension solids can be up to 99.8%, the biochemical oxygen demand (BOD) can be up to 98.7%, the chemical oxygen demand (COD) removal rate can be up to 98%, the industrial sewage treatment agent is less in sludge amount, free of secondary pollution, stable in treatment effect, environment-friendly, and non-toxic.

The invention relates to immunogenic compositions comprising mutant Streptococcus pneumoniae pneumolysin proteins. The invention further relates to such proteins and nucleic acids encoding these proteins. In particular embodiments, the invention is directed to an isolated mutant pneumolysin (PLY) protein, wherein the mutant PLY protein differs from the wild type PLY protein by the presence of a mutation within the region of amino acids 144 to 161 of the wild type sequence, such that the toxicity of the mutant is reduced relative to that of the wild-type protein. In particular embodiments, the mutant PLY protein differs from the wild type protein by the substitution or deletion of amino acids within this region, including the deletion of two adjacent amino acids within the region of amino acids 144 to 151 of the wild type sequence.

The invention relates to the technical field of biology and particularly relates to paracoccus denitrificans and an application thereof in treatment of livestock farm wastewater. The paracoccus denitrificans TDFQ-1 is collected at the China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC NO:M2017238. The bacterial strain has the advantages of being high in growth speed, high in environmental tolerance and good in growth in an organic culture medium. The paracoccus denitrificans has a heterotrophic nitrification capability and an aerobic denitrification capability, and is capable of reducing harmful substances, such as ammonia nitrogen and nitrate in water, especially removing the ammonia nitrogen in the livestock farm wastewater with relatively high organic matter content and improving the breeding environment. The bacterial strain is capable of efficiently degrading fluoroquinolone antibiotics when treating the wastewater, reducing the pollution of the antibiotics to soil and water sources and protecting the human health.

The invention discloses a Comamonas testosteroni LH-N5 CGMCC No.6975. The invention also discloses a heterotrophic nitrification-aerobic denitrification microbial inoculum which comprises the Comamonas testosteroni LH-N5, or one or both of Achromobacter xylosoxidans subtype xylosoxidans LH-N25 and Paracoccus aminovorans LH-N40; and the strains are combined in any proportion. The invention also discloses a preparation method of the microbial inoculum. The microbial inoculum disclosed by the invention can solve the problem of difficulty in removing total nitrogen in the traditional reactor, can effectively remove ammonia nitrogen and total nitrogen in the water body in one reactor, has tolerance or degradation capability for phenols, amines, heterocycles, cyanides, polycyclic aromatic hydrocarbons and other toxic substances in wastewater, is especially applicable to nitrogenous chemical wastewater, has the advantages of simple treatment process, stable effect and environmental toxicant impact resistance, and has very wide application prospects in various actual nitrogenous chemical wastewater treatment processes.

The invention relates to the technical field of microbes, and especially discloses a composite microbial preparation used for river treatment. The composite microbial preparation used for river treatment comprises a compound microorganism, and the compound microorganism is composed of the following raw materials in percent by weight: 1-2% of nitrosococcus, 5-8% of thiobacillus thiooxidans, 8-15% of paracoccus denitrificans, 20-30% of bacillus spp, 8-18% of rhodospira, 6-15% of lactobacillus spp, 10-15% of saccharomyces cerevisiae, and 20-30% of bacillus subtilis. After being poured into polluted water, the composite microbial preparation used for river treatment is capable of obviously improving the water body blackening smelliness problem, reducing water-quality ammonium nitrogen and total phosphorus content, decomposing hydrogen sulfide and other harmful substances and improving water body environment. The composite microbial preparation used for river treatment is capable of improving the absorption utilization efficiency of water plants on nutrition elements and obviously improving the water body transparency, and possesses the advantages of stabile microbial preparation, strong purification capability, simple preparation, low cost and applicability to river treatment.

Belonging to the field of microbial environmental protection, the invention provides a microbial agent for treatment of sewage and sludge. The microbial agent mainly includes Rhodospirillum rubrum, rhodopseudomonas palustris, bacillus subtilis, Streptomyces microflavus, streptococcus thermophilus, candida utilis, Streptomyces viridis, pseudomonas aeruginosa, virgibacillus halodenitrificans, and Paracoccus denitrificans 10 bacteria. Each bacterium is subjected to conventional slant culture, shaking culture and seed tank cultivation respectively, the bacteria in the seed tank are mixed and then put into sewage directly, symbiosis of flora is utilized to reach the purpose of treating sewage and reducing sludge generation. The microbial agent provided by the invention can be applied to large-scale production and direct adding, is low in cost, and can adapt to changeful sewage components. And the flora has good impact resistance to sewage.

The invention belongs to the technical field of biology and environment protection, and aims at providing paracoccus denitrificans and a method for disposing single-cell protein through high ammonia-nitrogen wastewater. The adopted technical scheme is as follows: the invention provides hydrogen-oxidizing bacteria, namely paracoccus denitrificans Y5, which is collected in General Microorganisms Center of China Committee for Culture Collection of Microorganisms on May 12, 2017 (address: Institute of Microbiology of Chinese Academy of Sciences, No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, 100101), with preservation number CGMCC No. 14118. The paracoccus denitrificans Y5 provided by the invention can remove COD and ammonia nitrogen in high ammonia-nitrogen wastewater, fix production of single-cell protein (SCP) by CO2, and can remove stink of wastewater.

The present invention relates to a new strain of coronavirus and its application. Said invention utilizes the throat-swabbed cell colture of SARS patient to separate out a new type coronavirus, called by name of SARS coronavirus Fudan I strain. By infecting Vero E 6 cell and patient's serum the indirect FA display is positive, after the influenza virus A and B, RS virus and pathogens of pneumococcus, etc. are excluded, the tests show that said separated coronavirus is a variant strain of new coronavirus. Said strain can be used for providing basis for preparing SARS diagnosis reagent and researchnig and producing medicine for resisting and curing SARS disease.

The invention discloses an efficient sewage treatment microbial agent. The efficient sewage treatment microbial agent is prepared from, by weight, 2-4 parts of nitrosobacteria, 10-20 parts of bacillus subtilis, 8-15 parts of alcaligenes faecalis, 5-10 parts of bacillus megatherium, 2-8 parts of pseudomonas aeruginosa, 2-10 parts of candida lipolytica, 2-8 parts of deoxidized paracoccus, 2-6 parts of pseudomonas stutzeri, 2-10 parts of saccharomyces cerevisiae, 1-4 parts of aspergillus niger, 0.1-0.6 part of yeast extract, 2-6 parts of peptone, 0.1-0.5 part of glucose, 8-15 parts of kaolin, 2-5 parts of aluminum polychlorid, 0.1-0.5 part of FMEE and a proper amount of water. The efficient sewage treatment microbial agent is suitable for treating industrial waste water, breeding waste water and the like, all strains are in cooperation, symbiotic coordination is achieved, high activity is achieved, and reproduction is fast. The efficient sewage treatment microbial agent is put in the waste water to be treated, the degradation effect can be effectively accelerated, the treatment water quality can be greatly improved, operating cost can be reduced, the treated water reaches the standard to be drained, and damage to environment is reduced.

The invention discloses a salt-tolerant pyrene degrading bactericide applied to oil-containing silt. The salt-tolerant pyrene degrading bactericide is characterized by comprising Paracoccus sp. PH205 and a carrier; the Paracoccus sp. PH205 is preserved in the common microorganism center of China Committee for Culture Collection of Microorganisms on May 16th, 2014, and the preservation number is CGMCC No. 9174. Polycyclic aromatic hydrocarbon pyrene in the salt-containing and oil-containing silt can be degraded quickly by the aid of the salt-tolerant pyrene degrading bactericide; As proved by experiments, the salt-tolerant pyrene degrading bactericide has the advantages that the salt-tolerant pyrene degrading bactericide is high in pyrene degrading speed and wide in salinity application range, and can be used for the oil-containing silt with the salinity of 5% at most, and the content of the polycyclic aromatic hydrocarbon pyrene in the oil-containing silt can be reduced effectively.

The invention discloses a new strain HT of pediococcus acidilactici and a feed additive containing bacillus like-bacteriocin and pediococcus acidilactici. The collection number of the new strain of pediococcus acidilactici is CGMCC No.6500. The freeze-dried powder of the like-bacteriocin of bacillus subtilis CGMCC No.6511 is sufficiently mixed with the powder of pediococcus acidilactici HT according to a proportion to obtain the feed additive. The feed additive is a green feed additive which improves the feed conversion rate, enhances the productivity and immunity of animals, prevents diarrhea, and realizes an effect of improving the quality of animal products.

The invention discloses pediococcus acidilactici P2CGMCC No.4213 and a method for producing pediocin by using the same. The method comprises the following steps of: culturing and activating a pediococcus acidilactici strain P2 and inoculating to an improved Man Rogosa Sharpe (MRS) culture medium for fermentation culture, wherein in the fermentation culture process, the activated pediococcus acidilactici strain P2 is inoculated to the improved MRS fermentation culture medium, the fermentation temperature is between 35 and 37 DEG C, the pH value is 5.5 to 7.5, culture time is 15 to 22 hours, inoculation amount is 0.3 to 0.6 percent, 20 to 25 percent of dissolved oxygen is kept in fermentation liquid, and glucose is fed; and extracting the pediocin from a fermentation product after culturing for 15 to 22 hours. The fermentation is performed through the improved MRS culture medium under the optimized fermentation culture condition, so that the yield of the pediocin is over 22,000AU / ml; and by combining a method for extracting the non-secreted pediocin from cells, the total yield of the pediocin is over 27,500AU / ml, and the pediocin is suitable for large-scale industrial production.

The invention relates to a pediococcus pentosaceus G11 strain as well as screening and applications of the pediococcus pentosaceus G11 strain. The pediococcus pentosaceus G11 strain is preserved in the China Center for Type Culture Collection, and is assigned with the accession number of CCTCC NO:2016248. The pediococcus pentosaceus G11 strain has tolerance to compound sulfamethoxazole and ceftazidime, has antibacterial activity for vibrio parahaemolyticus, aeromonas hydrophila, vibrio alginolyticus, staphylococcus aureus and beta streptococcus, has certain stress resistance and enzyme producing ability, is free of hemocyte solubility, and has no drug tolerance for most antibiotics; the dipping bath experiment proves that the pediococcus pentosaceus G11 strain is harmless to the host, can improve the immunity of the body, has wide application prospects, can be applied to probiotic preparations, and also can be prepared into bacterial powder which is then added into a feed as a feed additive, further, the growth of pathogenic bacteria in the bodies of the animals is inhibited, so that the death rate of animals is reduced, and the pediococcus pentosaceus G11 strain is a probiotic brain worthy of development.