9 results about "Synechococcus" patented technology

The invention discloses a function unannotated protein CpsA and application thereof, and belongs to the technical field of biochemistry. The CpsA protein provided by the invention is a thylakoid membrane protein which is coded by synpcc79421022 in synechococcus slender PCC 7942, and the function of the thylakoid membrane protein is not annotated. The protein can be specifically combined with an early PSII assembly factor Psb28, formation and stability of a PSII final oxygen evolution complex are promoted, and the assembly efficiency of a light system II and formation of a functional complex are remarkably improved. In addition, the CpsA protein is highly conserved in cyanobacteria, microalgae and land plants, and has wide application potential.

The invention provides an iron electrolysis coupled phycomycete microbial fuel cell, a preparation method and application thereof, and a wastewater treatment method, and belongs to the technical field of wastewater treatment. The IE-MFC comprises an anode chamber, a cathode chamber and an iron electrolysis chamber which are sequentially arranged, the anode chamber and the cathode chamber are separated by a proton exchange membrane, the cathode chamber and the iron electrolysis chamber are separated by a cation exchange membrane, and an anaerobic microbial membrane is fixed on the surface of an anode; and the surface of the cathode is fixed with coleus. An iron electrolysis and phycomycete symbiotic system is introduced, the metabolic activity of microorganisms can be stimulated and strengthened through a micro electric field generated inside, the biological metabolism of phycomycetes and the synergistic effect of the electric field of the phycomycetes decompose sulfamethoxazole, and meanwhile ferrous ions are generated in situ through iron electrolysis; and oxygen generated by photosynthesis of the coleus in the cathode region is triggered to generate hydroxyl free radicals with strong oxidizing property, so that mineralization of refractory intermediate products is realized, and a'biodegradation-micro electric field-iron electrolysis' three-synergistic degradation mechanism is formed.

The application discloses a method for treating dairy farm wastewater based on Synechococcus, Aspergillus and green algae. In the wastewater treatment method, Aspergillus is used in the first step, and can realize rapid conversion of organic matter in dairy farm wastewater. Synechococcus is used in the second step, and has strong treatment capacity for high-concentration wastewater in a dairy farm, and is named Synechococcus ZSL-NS1. On the basis of the first two steps, the biomass can be rapidly increased. Finally, the removal rate of pollutants in the dairy farm wastewater can reach more than 80%, and the biomass yield is increased by 2.3 times. In addition, Synechococcus ZSL-NS1 can also produce chlorophyll, protein, polysaccharide, oil and other biomasses, so that the purpose of recycling is achieved while the pollution is removed, and the method has application value.

The invention discloses a method for improving the photosynthetic efficiency of blue-green algae. The method comprises the following steps: enabling the blue-green algae to over-express an sll5097 gene with the GenBank number of BAD01867.1; the synechocystis overexpression sll5097 gene can grow faster, and the physiological phenotype is better.

The invention provides a polyphosphate kinase overexpression synechocystis and an application thereof in cadmium pollution treatment. An overexpression vector containing a strong promoter and a polyphosphate kinase gene sll0290 is introduced into synechocystis to obtain a synechocystis mutant, algae slurry rich in polyphosphors is obtained through a synechocystis mutant algae species grading expanding culture and large-scale culture method, and then the algae slurry is used for treating cadmium-polluted soil environments such as rice fields. The concentration of bio-available cadmium ions in the soil can be obviously reduced. When the polyphosphate kinase overexpression synechocystis is applied to treatment of cadmium-polluted soil such as rice fields, the stable, long-acting and safe synergistic effect is achieved.

The invention discloses a buoyancy capsule based on slow release of 3-hydroxydecanoic acid as well as a preparation method and application of the buoyancy capsule. The capsule comprises an air / inert gas core, a biodegradable polymer shell layer and 3-hydroxydecanoic acid loaded in the shell layer, the apparent density of the capsule is lower than or close to that of water, and the capsule can suspend on the surface layer of a water body for a long time and continuously release 3-hydroxydecanoic acid, so that the concentration of 3-hydroxydecanoic acid is maintained at an effective window of 1-2 ppm, thereby inhibiting growth of blue-green algae for a long time and reducing recurrence. The preparation method adopts coaxial gas-liquid injection to form gas-nucleus liquid drops and solidify the gas-nucleus liquid drops, and has the advantages of monodisperse particle size, adjustable hollow rate, greenness, no residue and the like. The buoyancy capsule prepared by the invention can inhibit the growth of blue-green algae in a water body for a long time, is suitable for water environments such as lakes, reservoirs, landscape water bodies and the like in which the blue-green algae are easy to gather on the surface layer, and is particularly used for inhibiting the proliferation and water bloom formation of dominant blue-green algae such as synechococcus, synechocystis and microcystis aeruginosa.

The invention discloses a compound microbial organic fertilizer and a preparation method thereof, and belongs to the technical field of compound organic fertilizers, the compound microbial organic fertilizer comprises livestock and poultry manure, leaf mold, lignite, seaweed residues, synechococcus, a compound fermentation inoculant, diatom ooze, turf, porous pelelith powder, modified charcoal and living adult earthworms. By means of secondary fermentation and curing treatment, the number of thalli of the finished fertilizer is increased, flora in earthworms is utilized, nutrient activity and soil air permeability are improved, vesuvianite powder, modified charcoal and graphene are adopted, the air permeability, water retention and fertilizer retention effects of soil after fertilization are improved, pesticide residues are effectively adsorbed, slow degradation is achieved, and tea seed cakes and lignite are adopted, so that the effect of improving the fertilizer efficiency is achieved. The soil pH is effectively adjusted, soil-borne diseases are inhibited, and the soil aggregate structure is improved in cooperation with seaweed residues and synechococcus.

The present application relates to the technical field of coral symbiotic cyanobacteria and cyanobacteria application, and specifically discloses a coral-derived cyanobacterium, an agricultural preparation, and an application and a method for improving the heat tolerance of corals. Synechococcus The present application discloses a coral-derived cyanobacterium GXU02, which is classified and named as: CCTCC NO: M 2026250, and is preserved in the China Center for Type Culture Collection (CCTCC). The present application isolates a cyanobacterium from the cross-petal coral, which provides good research materials for studying the function of marine cyanobacteria and the symbiotic relationship between the algae and corals, and has application potential in the development of preparations for crop growth promotion and stress resistance, and in the artificial modification of corals and the repair of coral reef ecosystems. The cyanobacterium of the present application can promote plant growth and improve stress resistance; inoculation of the cyanobacterium of the present application can improve the heat tolerance of corals, effectively alleviate the crisis of coral heat whitening, and open up a new idea and method for the cultivation of heat-resistant seedlings for the repair of coral reef ecosystems based on the recombination of symbiotic organisms.

This invention provides a method for constructing a multi-pond artificial wetland system based on rhizosphere synergistic symbiosis and its application, relating to the field of ecological environment restoration technology. Specifically, it includes the following steps: preparing engineered algal strain A displaying laccase on its surface and engineered algal strain B displaying non-specific nucleases on its surface, and mixing them in a specific ratio to prepare a composite microalgae agent; introducing the prepared composite microalgae agent into a shallow pond at the front end of the wetland to construct a photocatalytic microalgae pretreatment unit; and planting submerged plants in the symbiotic ponds connected in series after the pretreatment unit. This invention, by employing a multi-pond artificial wetland system construction method based on rhizosphere synergistic symbiosis and utilizing synthetic biology surface display technology, anchors bacterial laccase and non-specific nucleases onto the cell surfaces of different Synechococcus algae, solving the problem of insufficient carbon source leading to easy saturation and short lifespan of the substrate adsorbed with phosphorus in existing artificial wetlands treating wastewater effluent, and compensating for the shortcomings of existing single ecological restoration technologies.