9results about How to "Biologically active and stable" patented technology

The invention relates to the technical field of freeze-dried preparations, and provides exosome freeze-dried powder as well as a preparation method and application thereof. The exosome freeze-drying preparation is prepared by mixing an exosome solution and a freeze-drying protection solution and then freeze-drying the mixture, the concentration of the exosome solution in the mixed solution is 0.1-10% V / V, and the freeze-drying protection solution comprises 0.4-4% W / V of saccharides and 0.25-4% W / V of alcohols; the saccharide is trehalose, cane sugar or lactose; the alcohol is mannitol, sorbitol or a combination of mannitol and polyethylene glycol; and the solvent is sterilized water for injection or a PBS buffer solution. The exosome freeze-dried powder is stable in activity, small in irritation, good in safety and good in fibroblast proliferation promoting capacity, when the exosome freeze-dried powder is applied to supporting essence or masks in the medical beauty industry, the exosome activity of the exosome freeze-dried powder is still kept stable, and the application prospect is good.

The invention discloses 3D printing biological material ink based on a drug sustained-release polycitrate material and a preparation method and a printing method of the 3D printing biological material ink, and relates to the technical field of 3D printing biologication.The 3D printing biological material ink is prepared from a medical polycitrate polymer, nano-hydroxyapatite and an active drug ingredient; the addition amount of the hydroxyapatite is 5-65% of the total mass of the obtained biological material ink; the active pharmaceutical ingredients are gastrodin, notoginsenoside, recombinant wnt3a protein and LL-37 peptide; when the active medicines are gastrodin and notoginsenoside, the addition amount of the active medicines is 4-12% of the total mass of the obtained biological material ink; when the used active drugs are recombinant wnt3a protein and LL-37 peptide, the addition amount of the active drugs is 0.001-0.002% of the total mass of the obtained biological material ink; and the particle size of the nano hydroxyapatite is 60-80 nm. According to the method, 3D printing process parameters are selected, post-treatment is adopted to adjust a cross-linked network structure of a poly-citrate polymer, biological material scaffolds with different mechanical properties and degradation properties are obtained, and stable and long-acting release of carried drugs is ensured.

The present application relates to the field of microorganisms, and specifically discloses a lactic acid bacterium and its application in preventing and controlling bacterial infections in livestock and poultry. The lactic acid bacterium is produced by fermentation of Lactococcus lactis. The strain is named Lactococcus lactis 5019 and was deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration on January 13, 2021. The deposit address is: Beijing, China, and the deposit number is CGMCC NO.21607. The lactic acid bacterium produced by Lactococcus lactis in the present application can be used to prevent and control bacterial infections in livestock and poultry, and it has the advantages of high temperature resistance and strong antibacterial activity.

The invention relates to a polysaccharide-assisted tea extract as well as a preparation method and application thereof. Fresh tea leaves are soaked with an aloe polysaccharide solution, tea leaves obtained through filtration are dried in the sun to obtain dry tea leaves, then the dry tea leaves are soaked with filtrate obtained through filtration again and then filtered, the tea leaves are subjected to pure water soaking and high-temperature and high-pressure treatment to obtain tea leaf liquid, and the tea leaf liquid is subjected to concentration, pure water hydrolysis and high-temperature and high-pressure treatment to obtain hydrolyzed tea leaf liquid. And mixing the filtrate obtained by filtering the hydrolyzed tea liquid with the filtrate obtained in the previous step, filtering, concentrating and drying. The ethanol aqueous solution can dissolve soluble substances of the aloe polysaccharide, and insoluble impurities are removed in the subsequent filtering step. According to the method, effective substances of the tea leaves are extracted under the assistance of the polysaccharide, the EGCG content in the tea leaves can be greatly increased, and the anticancer activity of the tea extract is improved through the synergistic effect of the aloe polysaccharide and active substances in the tea extract.

The utility model provides a centrifugal device for cell protein detection relates to cell protein detection field, including centrifugal unit, including the outer cover, the inner chamber of setting in the outer cover, the inner chamber of setting in the inner chamber of the cover that is connected in the outer cover top through the hinge activity, centrifugal assembly for carrying out centrifugal operation of setting in the inner chamber of the inner chamber of the inner chamber of setting, and the microcontroller of setting in the outer cover front, temperature control unit, including the base, the radiating fan of setting in the base inner chamber one side, the utility model discloses through temperature sensor real -time monitoring the temperature in the inner chamber cavity, and cooperate microcontroller can make the synergistic operation of semiconductor refrigerating sheet and radiating fan, can rapidly reduce and maintain the temperature in the inner chamber cavity, ensure that the protein sample keeps stable biological activity in the centrifugal process, through centrifugal assembly makes cell fragments, cell organ and so on solid particle and protein solution can more effectively separate, has improved the purity of protein.

The present invention belongs to the field of bioengineering technology, and specifically relates to a kit and a detection method for detecting respiratory syncytial virus infection. The present invention first provides a respiratory syncytial virus F recombinant protein, which is based on the respiratory syncytial virus F wild protein sequence, replaces the Furin restriction site and p27 polypeptide between the F1 fragment and the F2 fragment of the wild protein sequence with a (Gly)8 flexible connecting peptide sequence, and optimizes the codons. Then, an application based on the recombinant protein is provided.

The invention relates to application of microorganism-derived outer membrane vesicles in medicines for treating obesity and related metabolic diseases, and belongs to the technical field of exosomes. In order to solve the technical problems that probiotics beneficial to intestinal tracts are weak in colonization ability in the intestinal tracts, are easily influenced by gastrointestinal tract environments and are unstable in individual response, the sphingosine-containing microbe-sourced outer membrane vesicle is provided, and the sphingosine content in the outer membrane vesicle is 50-65 ng / mg.

The invention discloses a heterocyclic ring-containing thiourea ligand and a preparation method and application thereof, and compared with the prior art, the heterocyclic ring-containing thiourea ligand has the following advantages: selection and optimization of a monothiourea skeleton: on the basis of early-stage work, the monothiourea skeleton is still kept in a ring shape, which is a precondition for keeping high activity and stability; on the basis that monothiourea and five-membered ring chain hydrocarbon skeleton structures are reserved, a previous N-connected benzene ring side chain is assumed to be further studied and optimized into an aliphatic carboxylic acid side chain, a functionalized aromatic ring is combined on a five-membered ring chain hydrocarbon skeleton, and the water solubility of thiourea ligand molecules is changed by changing the structure of the novel thiourea ligand, so that the water solubility of the thiourea ligand molecules is improved. The steric hindrance and the electronic effect can generate controllable influence on the catalytic efficiency and the recoverability of the thiourea ligand.

The present application provides a Cistanche deserticola polysaccharide and Hippophae rhamnoides polypeptide composition with hypoxia tolerance activity, and the amino acid sequence of the Hippophae rhamnoides polypeptide is SEQ ID NO:1. The composition provided by the present application can significantly prolong the survival time of mice, is superior to single components and positive controls, and exhibits synergistic effect. Molecular docking analysis shows that the used polypeptide has high affinity with SIRT1, supporting its mechanism in optimizing cell energy metabolism and antioxidant stress. The composition is suitable for highland medical protection and adjuvant treatment of hypoxic diseases, and has the advantages of clear components and stable biological activity.