4results about How to "Biologically active and stable" patented technology

The invention relates to a polysaccharide-assisted tea extract as well as a preparation method and application thereof. Fresh tea leaves are soaked with an aloe polysaccharide solution, tea leaves obtained through filtration are dried in the sun to obtain dry tea leaves, then the dry tea leaves are soaked with filtrate obtained through filtration again and then filtered, the tea leaves are subjected to pure water soaking and high-temperature and high-pressure treatment to obtain tea leaf liquid, and the tea leaf liquid is subjected to concentration, pure water hydrolysis and high-temperature and high-pressure treatment to obtain hydrolyzed tea leaf liquid. And mixing the filtrate obtained by filtering the hydrolyzed tea liquid with the filtrate obtained in the previous step, filtering, concentrating and drying. The ethanol aqueous solution can dissolve soluble substances of the aloe polysaccharide, and insoluble impurities are removed in the subsequent filtering step. According to the method, effective substances of the tea leaves are extracted under the assistance of the polysaccharide, the EGCG content in the tea leaves can be greatly increased, and the anticancer activity of the tea extract is improved through the synergistic effect of the aloe polysaccharide and active substances in the tea extract.

The utility model provides a centrifugal device for cell protein detection relates to cell protein detection field, including centrifugal unit, including the outer cover, the inner chamber of setting in the outer cover, the inner chamber of setting in the inner chamber of the cover that is connected in the outer cover top through the hinge activity, centrifugal assembly for carrying out centrifugal operation of setting in the inner chamber of the inner chamber of the inner chamber of setting, and the microcontroller of setting in the outer cover front, temperature control unit, including the base, the radiating fan of setting in the base inner chamber one side, the utility model discloses through temperature sensor real -time monitoring the temperature in the inner chamber cavity, and cooperate microcontroller can make the synergistic operation of semiconductor refrigerating sheet and radiating fan, can rapidly reduce and maintain the temperature in the inner chamber cavity, ensure that the protein sample keeps stable biological activity in the centrifugal process, through centrifugal assembly makes cell fragments, cell organ and so on solid particle and protein solution can more effectively separate, has improved the purity of protein.

The invention relates to application of microorganism-derived outer membrane vesicles in medicines for treating obesity and related metabolic diseases, and belongs to the technical field of exosomes. In order to solve the technical problems that probiotics beneficial to intestinal tracts are weak in colonization ability in the intestinal tracts, are easily influenced by gastrointestinal tract environments and are unstable in individual response, the sphingosine-containing microbe-sourced outer membrane vesicle is provided, and the sphingosine content in the outer membrane vesicle is 50-65 ng / mg.

The present application provides a Cistanche deserticola polysaccharide and Hippophae rhamnoides polypeptide composition with hypoxia tolerance activity, and the amino acid sequence of the Hippophae rhamnoides polypeptide is SEQ ID NO:1. The composition provided by the present application can significantly prolong the survival time of mice, is superior to single components and positive controls, and exhibits synergistic effect. Molecular docking analysis shows that the used polypeptide has high affinity with SIRT1, supporting its mechanism in optimizing cell energy metabolism and antioxidant stress. The composition is suitable for highland medical protection and adjuvant treatment of hypoxic diseases, and has the advantages of clear components and stable biological activity.