4817 results about "Biological activity" patented technology

A recombinant humanized collagen and a preparation method therefor. The recombinant humanized type III collagen can be efficiently and stably expressed in three expression systems of yeast, Escherichia coli, and CHO cells, and has the biological activity superior to that of a commercially available collagen product. The recombinant humanized type III collagen has structural characteristics and functional activity similar to those of a natural collagen, and thus has wide application prospects in biomedical materials, tissue engineering products, cosmetics, foods, health care products or drugs, medical instruments, medical cosmetology and the like.

The present invention discloses a human serum albumin-targeting nanobody and its application. A variety of human serum albumin-targeting nanobodies that can be used to construct long-acting fusion protein drugs and their immune screening methods are disclosed. The antibody is composed only of the heavy chain variable region and can be fused and expressed with therapeutic proteins or polypeptide drugs such as interleukin, interferon, and tumor necrosis factor in an Escherichia coli expression system. The fusion protein has the activity of specifically binding to human serum albumin, has a relatively high affinity, can effectively extend the drug half-life, and does not affect the biological activity of the recombinant protein drug at the same time.

The invention relates to the field of preparation and application of biological peptides, in particular to a hippocampal swelling peptide and application thereof in preparation of functional products for repairing male reproductive system injury. A green controllable enzymolysis technology is utilized to obtain protein polypeptide with nutritional, health-care and medicinal values, five active peptide sequences with high biological activity are obtained through further research on the basis, the high-purity active peptide is obtained through a chemical synthesis mode, and the biological activity of the peptide is verified through mouse spermatogonium.

The present disclosure relates to systems, non-transitory computer-readable media, and methods for utilizing language machine learning model (LLM) as autonomous reasoners to navigate and execute multiple layers of a computerized bio-activity discovery pipeline of a tech-bio exploration system. In particular, the disclosed systems can utilize an LLM that learns to access one or more tech-bio exploration tools to execute one or more processes and / or tasks in a bio-activity discovery pipeline. For instance, the disclosed systems can provide an interactive query prompt interface to enable users to provide tech-bio queries (as prompts) and utilize the LLM with the prompts to execute one or more tasks in the bio-activity discovery pipeline to generate and / or retrieve bio-activity data for the query. Moreover, the disclosed systems can utilize one or more LLMs to autonomously utilize and / or interact with one or more tech-bio tools in the bio-activity discovery pipeline to generate and / or obtain bio-activity data.

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

The invention relates to the technical field of soil fertility query analysis, and discloses a citrus orchard soil fertility query method and system based on artificial intelligence. The method comprises the following steps: collecting surface and deep soil samples in a citrus orchard for physical and chemical detection, and filling missing values with space-time dual interpolation to obtain a data set; extracting physicochemical and biological activity features, and forming feature vectors by adopting a fusion strategy; inputting a cascade forest model to carry out five-level classification; classifying the sampling points to generate a distribution diagram; intelligent diagnosis is carried out, the fertilization amount is calculated, and a differentiated fertilization recommendation map is generated. According to the application, soil fertility accurate evaluation, spatial distribution visualization and differentiated fertilization recommendation based on multi-source data are realized, so that the fertilizer utilization rate is improved, the environmental risk is reduced, and the citrus yield and quality are improved.

The invention relates to the technical field of termite monitoring and killing, in particular to a termite monitoring and killing method based on multi-modal sensing and a dynamic threshold value, and adopts the technical scheme that multi-source signal acquisition is adopted, and biological activity signals of a target area are synchronously acquired through a vibration sensor, a sonic sensor, a chemical gas sensor and a thermal imaging sensor; the anti-interference performance is improved, so that the false alarm rate is reduced; environmental parameter compensation is carried out, temperature, humidity and soil conductivity data are collected in real time, a dynamic calibration coefficient is generated to correct sensor original data, and environmental adaptability is improved; the method comprises the following steps: acquiring data of a current area, performing dynamic threshold calculation, inputting the acquired data into an LSTM-Transformer hybrid network, and outputting a self-adaptive early warning threshold of the current area so as to reduce the possibility of leak detection; through multi-stage early warning judgment, the real-time performance is improved, and the early warning delay is reduced; and starting a corresponding disposal process according to the early warning level, performing response execution, and narrowing the spraying range, thereby reducing pollution.

The invention relates to the technical field of ballast water treatment, in particular to a ballast water treatment-ultraviolet cooperative control method and system based on multi-parameter feedback, and the method comprises the steps: collecting turbidity, pressure gradient, ultraviolet light transmittance and biological activity data in real time through multiple types of sensors arranged at an inlet of a filter unit, the surface of a filter screen and an ultraviolet reactor; a composite control model fusing hydromechanics and optical characteristics is constructed, and closed-loop cooperation of aperture dynamic adjustment, ultraviolet dose compensation and directional backwashing is realized. The method is characterized in that an electrostriction filter screen is adopted to realize local aperture gradient control so as to balance flux and precision; an ultraviolet dose dynamic compensation algorithm is designed, and the irradiation efficiency is enhanced and improved in combination with a vortex flow field; and establishing a linkage optimization mechanism of the backwashing period and the service life of the equipment. A real ship verifies that the method can effectively reduce comprehensive energy consumption, can adapt to water quality fluctuation of different sea areas in the world, and meets the long-acting compliance requirement of the IMOD-2 standard.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application of the swelling hippocampal peptide in preparation of anti-fatigue products. Five active peptide sequences with high biological activity are obtained through research, animal behavioral experiments prove that the belly distention hippocampal peptide has a remarkable anti-fatigue effect and can prolong the swimming exhaustion time and enhance the muscle strength, the anti-fatigue effect of the belly distention hippocampal peptide is verified, and a new way is provided for development of anti-fatigue hippocampal products.

The invention discloses an immunoregulation hydrogel as well as a preparation method and application thereof, and relates to the technical field of medical materials, amino-derived cationic polysaccharide, carboxyl-derived anionic polysaccharide and dopa-modified protein biopolymer materials are used as biological matrix materials, spermidine is used as a bioactive functional component, and the immunoregulation hydrogel is prepared by a one-step method. The preparation method comprises the following steps: by taking a benzaldehyde-terminated high polymer material as a cross-linking agent, forming a first layer of dynamic reversible cross-linked network structure through Schiff base reaction; then calcium ions are introduced, and the calcium ions and carboxylate radicals are complexed and cross-linked to form a second layer of static irreversible cross-linked network structure which can be used for an ESD (Electro-Static Discharge) operation. By adopting a step-by-step cross-linking mode, the first-step cross-linked single-network hydrogel has injectability, self-repairability, tissue adhesiveness and an immune regulation function, and the second-step cross-linking further ensures stable and lasting performance, so that the problems that a submucosal injection material is easy to disperse, short in lasting time and poor in stability are solved. The immunoregulation function effect of the wound repair material is poor.

The invention includes a mutant T7 DNA ligase or a biologically active fragment thereof, which has greater activity than wild type T7 DNA ligase. The mutant T7 DNA ligase, or the biologically active fragment, has one or more substitutions differing from the wild type, as described more fully in the Summary. The preferred mutant T7 DNA ligase has at least one of the following mutations: E63K (SEQ ID NO:4), K73E (SEQ ID NO:6), K137E (SEQ ID NO:7), K174E (SEQ ID NO:9), E182K (SEQ ID NO:11), K210E (SEQ ID NO: 13), E243K (SEQ ID NO:15), D245R (SEQ ID NO: 17), E268K (SEQ ID NO:19), E272K (SEQ ID NO:21), E289K (SEQ ID NO:23), K295E (SEQ ID NO:25) and D336R (SEQ ID NO:27).

The invention relates to the technical field of image recognition, and discloses a carotid plaque stability and stroke risk prediction system fusing multiple modes, and the system comprises a data collection and fusion module, a feature extraction and association module, a risk assessment and layering module, an intervention decision module, and a report generation and feedback module. When carotid plaque stability and cerebral apoplexy risk prediction is carried out, plasma proteomics, radiomics and clinical data are synergistically integrated by establishing a multi-modal data fusion analysis framework, so that the limitation that a traditional method depends on a single data source is overcome; the plaque risk can be evaluated from multiple dimensions of biological activity and morphological features, the comprehensiveness and accuracy of risk prediction are improved, a more reliable diagnosis basis is provided for clinic, interaction and sensitivity influence among different modal features can be adaptively quantified by introducing dynamic feature correlation modeling and a real-time weight calibration mechanism, and the risk prediction accuracy is improved. And objectivity and consistency of risk assessment results are ensured.

The invention discloses a method for extracting extracellular vesicles from dried Sanguisorba officinalis rhizomes and application of the extracellular vesicles, and relates to the technical field of molecular biology and biomedicine.The method comprises the following steps that S1, rehydration is conducted, specifically, the dried Sanguisorba officinalis rhizomes are subjected to liquid nitrogen quick freezing and then ground into powder at the low temperature, the powder and hydration liquid are mixed according to the proportion of 1: 10 (w / v), the mixture is slowly stirred for 2-4 h at the temperature of 4 DEG C, and the extracellular vesicles are obtained; removing large-particle impurities by using sterile gauze or a filter screen, and collecting filtrate; according to the method, through the synergistic effect of multiple enzymolysis and in combination with cellulase, pectinase and the like, the degradation efficiency is remarkably improved, meanwhile, EDTA is chelated with metal ions, and the enzymolysis effect is enhanced. Short-time alternating ultraviolet light is introduced to activate'dormant 'vesicles, so that the yield and the biological activity are improved. Secondly, multiple protection mechanisms, such as vitamin C, EDTA (Ethylene Diamine Tetraacetic Acid), cane sugar, BSA (Bovine Serum Albumin) and an ultraviolet protective agent are adopted, so that the integrity and the biological activity of the vesicles are ensured.

The invention belongs to the technical field of organic matter preparation, and discloses an amide-containing isoxazoline derivative as well as a preparation method and application thereof. According to the invention, chemical modification and molecular design are carried out on the compound with an isoxazoline structure, and a substituent group is introduced to N of an amide group, so that a series of efficient compounds which can be used for killing insects or mites in agriculture or forestry and have excellent activity are obtained, and particularly, the compounds have very good biological activity on lepidoptera, hemiptera and other pests. When being used at an extremely low concentration, the compound disclosed by the invention also shows an excellent control effect on various pests or pest mites, has insecticidal or acaricidal activity in each stage (such as eggs, larvae (nymphs), pupae and adults) of the life cycle of the pests or pest mites, and has an excellent control effect on the pests or pest mites which have drug resistance to traditional insecticides or acaricides. And the composition also shows excellent control activity.

The invention includes a mutant Taq polymerase, which can significantly extend and amplify a target sequence where the extension conditions are time limited to as little as one second. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.

The invention provides a recombinant XVII type collagen and application thereof, and relates to the technical field of protein engineering. The novel recombinant human XVII type collagen is obtained by optimizing and screening collagen structural domain sequences of the human XVII type collagen and then combining the sequences. The recombinant human XVII type collagen is consistent with the corresponding part of the amino acid sequence of the human XVII type collagen, does not generate immunological rejection when being applied to a human body, and can be widely applied to the industries of surgical medical treatment and medical beauty. In addition, the recombinant human XVII type collagen is high in stability, and the problem of protein degradation in the process of expressing recombinant protein by pichia pastoris can be avoided. In addition, the recombinant human XVII type collagen also has the effects of high biological activity, high biological safety, promotion of cell proliferation and differentiation and promotion of hair growth, and can be applied to the field of cosmetics or medicines as a functional component.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application of the swelling hippocampal peptide in preparation of a product for improving memory ability and cognitive impairment. According to the invention, a green controllable enzymolysis technology is utilized to obtain protein polypeptide which has the main molecular weight of less than 1000 Da and has nutritional, health-care and medicinal values, and four active peptide sequences with high biological activity are obtained by further research on the basis of the protein polypeptide.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine prescription efficacy quantification method and system.The method comprises the steps that chemical component spectrums, pharmacodynamic substance contents and biological activity indexes of traditional Chinese medicines forming a prescription are obtained; constructing a multi-target regulation and control network by combining a traditional Chinese medicine-target interaction database according to the chemical component spectrum, the pharmacodynamic substance content and the biological activity index, identifying a core efficacy pathway cluster by adopting a Louvain algorithm, and calculating a pathway activation intensity value by adopting a pathway target membership weighting model; according to the core efficacy pathway cluster, establishing a mapping relation between the core efficacy pathway cluster and traditional Chinese medicine efficacy terms through ontology and semantic mapping, and according to pathway activation intensity values, generating a prescription efficacy intensity vector through a weighted aggregation model; according to the efficacy intensity vector of the prescription, verifying and correcting the network weight through an in-vitro cell model, and generating the efficacy quantification of the prescription in combination with the clinical syndrome calibration coefficient. Therefore, the problems of single data dimension and insufficient quantification result accuracy in the prior art are solved.

The invention relates to the technical field of synthetic biology, in particular to recombinant XVII type collagen as well as a preparation method and application thereof.The amino acid sequence of the recombinant XVII type collagen comprises a sequence shown in any one of SEQ ID NO.1-SEQ ID NO.3. The recombinant XVII type collagen can be highly expressed in an escherichia coli and pichia pastoris system, the expression system is simple, the cost is low, and the recombinant XVII type collagen is suitable for industrial production. The yield is high; the biological activity is good. The protein can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines, so that the protein can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like.

Described herein are novel and improved antibodies that bind to thymic stromal lymphopoietin (TSLP) and methods of use thereof. In certain aspects, described herein are methods of inhibiting TSLP biological activity. In certain aspects, the antibodies and methods described herein are used for treatment of an inflammatory disease or disorder associated with elevated levels of TSLP. In certain aspects, described herein are methods of treating asthma by administering anti-TSLP antibodies.

The invention belongs to the technical field of medicines, and particularly relates to application of eupatorium chinense extract in preparation of a medicine for treating gout. Researches show that the eupatorium chinense extract and the active components thereof have good biological activity and can inhibit the activity of xanthine oxidase (XOD). Molecular docking experiments also show that the eupatorium chinense extract and xanthine oxidase have good binding energy. Further research finds that the eupatorium chinense extract can reduce the levels of uric acid and creatinine in serum in a hyperuricemia mouse body, and has good uric acid reducing activity. In the field of treatment of hyperuricemia and gout, many traditional medicines have many adverse reactions. Compared with the prior art, the eupatorium chinense serving as a traditional Chinese medicine provides a safe and effective natural medicine for treating hyperuricemia and gout or gout complications due to the efficient uric acid reducing effect of the eupatorium chinense.

The invention belongs to the field of high polymer material chemistry, and particularly relates to double-network hydrogel based on sodium alginate / gelatin, a preparation method and dressing. A chemical method of grafting modification is adopted, grafting modification is carried out by designing abundant active groups on side chains of gelatin and sodium alginate, and based on traditional gelatin or sodium alginate hydrogel, not only is the thermal stability of gelatin improved, but also the biological activity of sodium alginate is improved, and the antibacterial property is achieved; the finally prepared SD / GelMA dual-network hydrogel wound dressing not only has excellent mechanical properties, but also has very high biocompatibility and antibacterial activity, and has huge application prospects and potential in the aspect of skin wound healing.

The present disclosure relates to methods that enable the continuous formation of droplets and dehydration of droplets to provide pharmaceutically relevant particles that can be used for therapy. In particular, the methods disclosed herein allow the controlled continuous droplet formation and dehydration that produce circular particles having low internal void spaces comprising bioactive therapeutic biologies.

The invention provides a recombinant humanized elastin with high biological activity as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering and biological materials. 100% of the amino acid sequence of the elastin is derived from human elastin and is prepared by expression of genetically engineered bacteria; the protein has a typical beta-spiral hydrophobic structural domain and a lysine-rich hydrophilic structural domain, has a good bionic structure and functional characteristics, can significantly promote proliferation, adhesion and migration of human skin fibroblasts, also has excellent antioxidant, anti-wrinkle and anti-aging activities, and can be used for preparing a biomimetic protein. The hydrogel can be widely applied to the fields of skin repair dressings, injection implants, artificial skin, biomedical materials, functional skin care products and the like; in addition, the elastin is good in solubility, high in stability, mature in expression system and suitable for large-scale production.

The invention discloses a metal ion-mediated composite polysaccharide-based steady-state probiotic loading body as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. Sodium alginate and metal ions are coordinated and self-assembled through interaction of non-covalent supramolecules to form a metal ion-alginate skeleton network, functional guar gum is further filled, nano-scale aggregates are pushed to be spontaneously deposited on the surfaces of thalli, and a compound polysaccharide coating with excellent adhesion performance and steady-state performance is generated, so that the stability of the composite polysaccharide coating is improved. The independent coating of the probiotics is realized. The presence of metal ions increases the affinity of the biological material to the coated probiotics, and guar gum endows the probiotic delivery system with good adhesion characteristics. According to the probiotic loading body, the operation steps of an existing cell surface modification technology are simplified, the density is improved while the usage amount of biological materials is reduced, cells can be protected from being damaged by the gastrointestinal tract stress environment so as to maintain the activity of probiotics, and the colonization capacity and the biological activity of the probiotics in the intestinal tract are improved.

The invention belongs to the technical field of soil improvement, and relates to a soil conditioner for saline-alkali soil and a soil improvement method. The soil conditioner comprises vinegar residues, chitin, glucose, a composite biological preparation, a modified gel and a functional additive, the composite biological agent comprises bacillus subtilis powder, bacillus licheniformis powder, trichoderma spore powder, brown algae powder and light calcium carbonate; the functional auxiliary agent comprises modified bentonite, polyglutamic acid, pregelatinized starch and biochar potassium humate. Through the synergistic effect of multiple components and structural design optimization, systematic remediation of physical properties, chemical properties and biological activity of the saline-alkali soil is achieved. According to the technology, the limitation of single function of a traditional improvement measure is broken through, and the long-standing technical problems of return salt rebound, low fertility, microbial community imbalance and the like in saline-alkali soil treatment are effectively solved by constructing a multi-layer and multi-dimensional soil improvement system.

The invention relates to the technical field of plant exosome-like extracts, in particular to a liquorice exosome-like extract as well as extraction and application thereof. The method comprises the following steps: firstly, removing impurities such as cells, cell debris, organelles, large vesicles and the like in homogenate by adopting a combination mode of low-speed centrifugation and medium-speed centrifugation, and further purifying the homogenate by adopting high-speed centrifugation after the low-speed centrifugation and the medium-speed centrifugation are completed, so as to obtain the exosome-like extract. And the setting of the increasing rotation speed is beneficial to finer separation of exosome-like particles with different sizes and densities, so that the purity and quality of the extract are improved. According to the method, all centrifugal operations are carried out in a low-temperature environment of 4 DEG C; the low-temperature condition is beneficial to maintaining the biological activity and stability of the sample, and the problems of protein denaturation, enzyme inactivation and the like caused by temperature rise are reduced, so that the quality and performance of the exosome-like extract are ensured.

The invention belongs to the technical field of pharmaceutical preparations, and relates to traditional Chinese medicine self-assembled nanoparticles for enhancing anti-inflammatory activity as well as a preparation method and application of the traditional Chinese medicine self-assembled nanoparticles. The traditional Chinese medicine self-assembled nanoparticles are prepared from biphenyl cyclooctene type lignan and an oleanane type pentacyclic triterpenoid compound, wherein the mass ratio of the biphenyl cyclooctene type lignan to the oleanane type pentacyclic triterpenoid compound is 1 to (0.125 to 8). Through an anti-solvent exchange method, the carrier-free nanoparticles based on self-assembly of the traditional Chinese medicine active ingredients are prepared by utilizing spontaneous non-covalent interaction between the biphenyl cyclooctene lignan and the oleanane pentacyclic triterpenoid, so that the cytotoxicity is remarkably reduced, the biocompatibility is enhanced, and the toxic and side effects are reduced; meanwhile, the traditional Chinese medicine self-assembled nano-particles have a remarkable cellular uptake effect, the absorption and utilization efficiency of the medicine is improved, and more efficient anti-inflammatory biological activity is shown.

The invention discloses a method for extracting and purifying flavone in litsea coreana through an ultrasonic-assisted eutectic solvent, and belongs to the technical field of green processing of natural products. The invention provides a method for efficiently preparing litsea coreana flavone by integrating computer prediction, green solvent extraction and resin purification technologies. The method comprises the following steps: firstly, calculating and screening a deep eutectic solvent system with the highest affinity with the litsea coreana flavone on the basis of a COSMO-RS theoretical model, then coupling the optimized DES with an ultrasonic-assisted extraction (UAE) technology, and through the synergistic effect of the DES and the UAE technology, remarkably improving the extraction rate of the litsea coreana flavone and furthest keeping the biological activity of a target component; then, a macroporous resin dynamic adsorption-desorption purification process is linked, so that high-selectivity enrichment and impurity removal of the flavonoid compounds in the crude extract are realized, a high-purity product is obtained, and a complete process route from intelligent screening, efficient extraction to precise purification is finally formed.

The invention relates to the technical field of pig feed, and discloses a low soybean meal type pig feed formula based on enzymolysis protein and a preparation method of the low soybean meal type pig feed formula based on enzymolysis protein, dephenolized cottonseed meal, corn alcohol lees and the like are used as main raw materials, and the core of the low soybean meal type pig feed formula is that gradient enzymolysis and directional fermentation are dynamically coupled. The probiotics are subjected to specific induction domestication, so that the probiotics can efficiently adapt to a reaction system; in the coupling process, compound cellulase, protease and the like are added step by step according to a time sequence, the pH value of the system is intelligently adjusted by utilizing acid generated by fermentation, and optimal conditions are created for subsequent directional enzymolysis, so that the functional peptide with specific molecular weight is enriched. And finally, a low-temperature keep-alive drying technology is adopted, so that the biological activity of probiotics and functional peptides in the product is completely reserved. According to the method, raw material harmlessness, protein directional conversion and active substance protection are integrated, and high-value utilization of low-value agricultural and sideline products is achieved.