6664 results about "Biological activity" patented technology

A recombinant humanized collagen and a preparation method therefor. The recombinant humanized type III collagen can be efficiently and stably expressed in three expression systems of yeast, Escherichia coli, and CHO cells, and has the biological activity superior to that of a commercially available collagen product. The recombinant humanized type III collagen has structural characteristics and functional activity similar to those of a natural collagen, and thus has wide application prospects in biomedical materials, tissue engineering products, cosmetics, foods, health care products or drugs, medical instruments, medical cosmetology and the like.

The present invention discloses a human serum albumin-targeting nanobody and its application. A variety of human serum albumin-targeting nanobodies that can be used to construct long-acting fusion protein drugs and their immune screening methods are disclosed. The antibody is composed only of the heavy chain variable region and can be fused and expressed with therapeutic proteins or polypeptide drugs such as interleukin, interferon, and tumor necrosis factor in an Escherichia coli expression system. The fusion protein has the activity of specifically binding to human serum albumin, has a relatively high affinity, can effectively extend the drug half-life, and does not affect the biological activity of the recombinant protein drug at the same time.

The invention relates to a high-activity recombinant XVII type collagen as well as a rapid preparation method and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 1, and the nucleotide sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 2. The invention also provides a preparation method and application of the recombinant XVII type collagen. The high-activity recombinant XVII type collagen has good biological activity, has good effects of promoting cell adhesion, cell proliferation and cell migration, and can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines. The high-activity recombinant XVII type collagen gene is integrated to different positions of a yeast genome through three different vectors, and the copy number of a target gene is increased, so that the expression quantity of the recombinant XVII type collagen is remarkably increased, and the yield of the high-activity recombinant XVII type collagen is effectively increased.

The invention relates to the field of preparation and application of biological peptides, in particular to a hippocampal swelling peptide and application thereof in preparation of functional products for repairing male reproductive system injury. A green controllable enzymolysis technology is utilized to obtain protein polypeptide with nutritional, health-care and medicinal values, five active peptide sequences with high biological activity are obtained through further research on the basis, the high-purity active peptide is obtained through a chemical synthesis mode, and the biological activity of the peptide is verified through mouse spermatogonium.

The invention discloses red ginseng fermentation liquor with high rare saponin content and functional bioactivity and a preparation method thereof, and the preparation method comprises the following steps: mixing red ginseng powder with water to obtain red ginseng mixed liquor; cellulase is added into the red ginseng mixed solution for enzymolysis, and red ginseng enzymatic hydrolysate is obtained; or heating the red ginseng liquid, and performing hot water extraction to obtain a red ginseng extracting solution; heating the red ginseng extracting solution to be boiled, and after sterilization is finished, adding lactic acid bacteria powder for fermentation after the red ginseng solution is cooled, so as to obtain red ginseng fermentation liquor; and carrying out solid-liquid separation on the fermentation broth to obtain a fermentation supernatant, and sterilizing the red ginseng fermentation broth to obtain the sterilized red ginseng fermentation broth. According to the preparation method disclosed by the invention, the content of total saponins in the red ginseng fermentation liquor is increased, the variety and content of rare saponins can be increased, and in addition, the prepared red ginseng fermentation liquor has relatively high antioxidant activity, tyrosinase inhibition rate activity, leukoproteinase inhibition activity and hyaluronidase inhibition activity; wide application prospects and market potentials are realized.

The invention provides a recombinant humanized III-type collagen with a triple helix structure and preparation and application thereof, and belongs to the technical field of protein engineering, the amino acid sequence of the recombinant humanized III-type collagen comprises n core units, n is an integer of 1 or greater than 1, and the amino acid sequence of each core unit is as shown in SEQ ID NO: 1; the invention also provides a construction method of the expression system and a preparation method of the recombinant humanized III-type collagen. The invention also provides practical industrial application according to the characteristics of the protein. The recombinant humanized III-type collagen provided by the invention is high in purity, good in stability and high in biological activity, and experiments show that the recombinant humanized III-type collagen has various biological activities of promoting cell proliferation and migration and the like.

A computer implemented method may collect a set of known drug compounds from a database, and create augmented datasets with labeled and unlabeled chemical data and biological data. A computer implemented method may train a neural network using the expanded training set to generate a prediction score of bioactivity of candidate drug compounds towards the biological target, wherein the contribution of the second plurality of unlabeled samples in training the neural network is scaled by a parameter. A computer implemented method may output one or more refined neural network models capable of generating candidate drug compounds with predicted activity. A computer implemented method may generate a candidate drug compound by inputting a candidate drug compound with chemical data into the trained neural network model.

The present disclosure relates to systems, non-transitory computer-readable media, and methods for utilizing language machine learning model (LLM) as autonomous reasoners to navigate and execute multiple layers of a computerized bio-activity discovery pipeline of a tech-bio exploration system. In particular, the disclosed systems can utilize an LLM that learns to access one or more tech-bio exploration tools to execute one or more processes and / or tasks in a bio-activity discovery pipeline. For instance, the disclosed systems can provide an interactive query prompt interface to enable users to provide tech-bio queries (as prompts) and utilize the LLM with the prompts to execute one or more tasks in the bio-activity discovery pipeline to generate and / or retrieve bio-activity data for the query. Moreover, the disclosed systems can utilize one or more LLMs to autonomously utilize and / or interact with one or more tech-bio tools in the bio-activity discovery pipeline to generate and / or obtain bio-activity data.

Compositions and methods relating to paraoxonase fusion polypeptides are disclosed. In some aspects, the fusions are bispecific molecules that include a first biologically active polypeptide linked amino-terminal to a biologically active paraoxonase, wherein the first biologically active polypeptide is a DNase, an RNase, a SOD1, a CTLA-4 extracellular domain, a CD40 extracellular domain, or a polypeptide that specifically binds and neutralizes an inflammatory cytokine. Bispecific fusions may further include a second biologically active polypeptide (e.g., a dimerizing or FcRn-binding domain) linked carboxyl-terminal to the first biologically active polypeptide and amino-terminal to the paraoxonase. In other aspects, a fusion polypeptide includes a biologically active paraoxonase linked carboxyl-terminal or amino-terminal to a dimerizing or FcRn-binding domain. Also disclosed are dimeric proteins comprising first and second paraoxonase fusion polypeptides as disclosed herein. The fusion polypeptides and dimeric proteins are useful in methods for therapy.

The invention discloses a bionic nanofiber-hydrogel composite biological scaffold as well as a preparation method and application thereof. The bionic nanofiber-hydrogel composite biological scaffold is a composite scaffold for simulating a gray-white matter partition structure of a spinal cord tissue, and comprises bionic nanofibers and a bionic hydrogel material, wherein the bionic nanofibers are arranged in an oriented manner and simulate a white matter ordered structure around the spinal cord, and the bionic hydrogel material is combined with the bionic nanofibers and simulates gray matter in the center of the spinal cord. According to the bionic nanofiber-hydrogel composite biological scaffold, the biological activity of loaded stem cells or adult cells can be improved, the problem that a large number of transplanted exogenous cells die due to unbalanced oxidative stress generated after tissue damage can be solved, and it is guaranteed that the transplanted cells reside in a damaged area and play a role; therefore, the spinal cord microenvironment is improved, proliferation and differentiation of endogenous neural stem cells are promoted, functional recovery after spinal cord injury is finally promoted, and great potential is achieved in spinal cord injury treatment.

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

The invention relates to the technical field of soil fertility query analysis, and discloses a citrus orchard soil fertility query method and system based on artificial intelligence. The method comprises the following steps: collecting surface and deep soil samples in a citrus orchard for physical and chemical detection, and filling missing values with space-time dual interpolation to obtain a data set; extracting physicochemical and biological activity features, and forming feature vectors by adopting a fusion strategy; inputting a cascade forest model to carry out five-level classification; classifying the sampling points to generate a distribution diagram; intelligent diagnosis is carried out, the fertilization amount is calculated, and a differentiated fertilization recommendation map is generated. According to the application, soil fertility accurate evaluation, spatial distribution visualization and differentiated fertilization recommendation based on multi-source data are realized, so that the fertilizer utilization rate is improved, the environmental risk is reduced, and the citrus yield and quality are improved.

The invention provides a high-stability recombinant XVII type collagen as well as a construction method and application thereof, and belongs to the technical field of bioengineering. According to the invention, an amino acid sequence of natural human (XVII) type collagen is selected as a basic unit and repeated for multiple times to obtain recombinant (XVII) type collagen; in the design process of the recombinant XVII type collagen, an unstable GXY triad is avoided, potential matrix metalloproteinase restriction enzyme cutting sites and glycosylation sites are abandoned, a KGD active sequence is reserved, and the probability of containing sites which are easy to degrade can be reduced; a sequence fragment of the recombinant XVII type collagen is derived from a full-length sequence of a natural collagen gene, has excellent solubility, biological activity and high stability, and can be kept stable in a high-temperature storage environment and under the condition of avoiding alkalinity; the product can be used as a raw material in the production process of medicines, medical equipment, biological materials or cosmetics, and has good practicability.

The invention relates to a bone organ constructed based on oxygen-releasing immune hydrogel microspheres and a preparation method and application thereof. An oxygen release material, macrophages and bone marrow mesenchymal stem cells are loaded in the oxygen release immune hydrogel microspheres. And adding the oxygen-releasing immune hydrogel microspheres into a culture medium for culturing to obtain the bone organ constructed based on the oxygen-releasing immune hydrogel microspheres. The oxygen release material (CaO2-HAp) is entrapped in the hydrogel microspheres, so that continuous oxygen supply can be provided in a defect area, the low-oxygen environment is improved, the activity of osteoblasts is improved, osteogenic differentiation is promoted, macrophages are entrapped by the microspheres, the immune microenvironment is regulated and controlled, the release of proinflammatory cytokines is reduced, the osteogenic efficiency is improved, and bone defect repair is accelerated. The micro-fluidic technology is utilized to accurately control the size and shape of the microspheres, so that the microspheres can adapt to irregular bone defects, bone organs are formed through long-term in-vitro culture, and the biological activity and adaptability of the bone repair material are improved.

Described herein are novel and improved antibodies that bind Interleukin 13 (IL-13) and methods of use thereof. In certain aspects, described herein are methods of inhibiting IL-13 biological activity. In certain aspects, described herein are pharmaceutical compositions comprising the anti-IL-13 antibodies. In certain aspects, the antibodies and methods described herein are used for treatment of an inflammatory disease or disorder associated with elevated levels of IL-13 and / or IgE.

The invention relates to the field of cubilose peptides, in particular to preparation and application of cubilose glycopeptides capable of preventing and assisting in treating gynecological inflammation and gastrointestinal inflammation. The invention provides cubilose glycopeptide which comprises cubilose peptide, sialylated O-glycan and sialylated N-glycan, and the cubilose peptide is connected with the sialylated O-glycan and / or the sialylated N-glycan. The cubilose glycopeptide provided by the invention has unique sialylated O-glycans and N-glycans, has unique biological activity and functions and synergistic effect compared with the cubilose glycopeptide without N-glycans or O-glycans and non-sialylated cubilose peptides, and forms and enhances the inhibition effect on pathogens and the antiviral effect; therefore, the compound has unique advantages in the aspect of preventing and treating related problems. The sialic acid binding type cubilose glycopeptide has stronger antibacterial activity, especially has an inhibition effect on pathogens such as candida albicans and helicobacter pylori, and also has an antiviral effect.

The invention relates to the technical field of termite monitoring and killing, in particular to a termite monitoring and killing method based on multi-modal sensing and a dynamic threshold value, and adopts the technical scheme that multi-source signal acquisition is adopted, and biological activity signals of a target area are synchronously acquired through a vibration sensor, a sonic sensor, a chemical gas sensor and a thermal imaging sensor; the anti-interference performance is improved, so that the false alarm rate is reduced; environmental parameter compensation is carried out, temperature, humidity and soil conductivity data are collected in real time, a dynamic calibration coefficient is generated to correct sensor original data, and environmental adaptability is improved; the method comprises the following steps: acquiring data of a current area, performing dynamic threshold calculation, inputting the acquired data into an LSTM-Transformer hybrid network, and outputting a self-adaptive early warning threshold of the current area so as to reduce the possibility of leak detection; through multi-stage early warning judgment, the real-time performance is improved, and the early warning delay is reduced; and starting a corresponding disposal process according to the early warning level, performing response execution, and narrowing the spraying range, thereby reducing pollution.

The invention discloses an algae-controlling bioactive preparation as well as a preparation method and application thereof. The preparation method comprises the following steps: inoculating bacillus velezensis HY16 in a bioactive substance fermentation culture medium and culturing; the culture temperature is 35-40 DEG C, and the culture time is 32-48 hours. The algae-controlling bioactive preparation provided by the invention has good capability of inhibiting growth of microcystis aeruginosa, and the algae-lysing rate of the algae-controlling bioactive preparation on microcystis aeruginosa in an aquarium system can reach 97% or above. The algae-controlling bioactive preparation prepared from the bacillus velezensis HY16 fermentation active liquid is stable in property, safe, environment-friendly and simple to apply, can be used for effectively and biologically controlling and repairing an aquarium water body with microcystis aeruginosa, is remarkable in effect, and has good market application prospect and value.

The invention relates to the technical field of ballast water treatment, in particular to a ballast water treatment-ultraviolet cooperative control method and system based on multi-parameter feedback, and the method comprises the steps: collecting turbidity, pressure gradient, ultraviolet light transmittance and biological activity data in real time through multiple types of sensors arranged at an inlet of a filter unit, the surface of a filter screen and an ultraviolet reactor; a composite control model fusing hydromechanics and optical characteristics is constructed, and closed-loop cooperation of aperture dynamic adjustment, ultraviolet dose compensation and directional backwashing is realized. The method is characterized in that an electrostriction filter screen is adopted to realize local aperture gradient control so as to balance flux and precision; an ultraviolet dose dynamic compensation algorithm is designed, and the irradiation efficiency is enhanced and improved in combination with a vortex flow field; and establishing a linkage optimization mechanism of the backwashing period and the service life of the equipment. A real ship verifies that the method can effectively reduce comprehensive energy consumption, can adapt to water quality fluctuation of different sea areas in the world, and meets the long-acting compliance requirement of the IMOD-2 standard.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application of the swelling hippocampal peptide in preparation of anti-fatigue products. Five active peptide sequences with high biological activity are obtained through research, animal behavioral experiments prove that the belly distention hippocampal peptide has a remarkable anti-fatigue effect and can prolong the swimming exhaustion time and enhance the muscle strength, the anti-fatigue effect of the belly distention hippocampal peptide is verified, and a new way is provided for development of anti-fatigue hippocampal products.

The invention relates to a biotechnology, and particularly discloses a plasmin degradation product D-dimer antibody and application thereof. According to the application, a single B cell antibody preparation technology and a flow cytometry sorting method are applied to the development of a blood coagulation in-vitro diagnosis fibrinolysis system detection antibody for the first time, and a mammalian cell expression system (293F) is adopted to successfully express and obtain three strains of D-dimer monoclonal antibodies (antibody numbers are DD-1, DD-2 and DD-3) with biological activity. Compared with the on-sale Roch D-dimer antibody, the three antibodies have the same affinity with the DD antigen, and can identify and detect the D-dimer in plasma with high specificity and high sensitivity; meanwhile, the probe has no cross reaction with fibrinogen, primary fibrinolytic substances (X, Y and D) and soluble fibrin (SF), and can be used for qualitative or quantitative detection of D-dimer.

The invention discloses an immunoregulation hydrogel as well as a preparation method and application thereof, and relates to the technical field of medical materials, amino-derived cationic polysaccharide, carboxyl-derived anionic polysaccharide and dopa-modified protein biopolymer materials are used as biological matrix materials, spermidine is used as a bioactive functional component, and the immunoregulation hydrogel is prepared by a one-step method. The preparation method comprises the following steps: by taking a benzaldehyde-terminated high polymer material as a cross-linking agent, forming a first layer of dynamic reversible cross-linked network structure through Schiff base reaction; then calcium ions are introduced, and the calcium ions and carboxylate radicals are complexed and cross-linked to form a second layer of static irreversible cross-linked network structure which can be used for an ESD (Electro-Static Discharge) operation. By adopting a step-by-step cross-linking mode, the first-step cross-linked single-network hydrogel has injectability, self-repairability, tissue adhesiveness and an immune regulation function, and the second-step cross-linking further ensures stable and lasting performance, so that the problems that a submucosal injection material is easy to disperse, short in lasting time and poor in stability are solved. The immunoregulation function effect of the wound repair material is poor.

The invention includes a mutant T7 DNA ligase or a biologically active fragment thereof, which has greater activity than wild type T7 DNA ligase. The mutant T7 DNA ligase, or the biologically active fragment, has one or more substitutions differing from the wild type, as described more fully in the Summary. The preferred mutant T7 DNA ligase has at least one of the following mutations: E63K (SEQ ID NO:4), K73E (SEQ ID NO:6), K137E (SEQ ID NO:7), K174E (SEQ ID NO:9), E182K (SEQ ID NO:11), K210E (SEQ ID NO: 13), E243K (SEQ ID NO:15), D245R (SEQ ID NO: 17), E268K (SEQ ID NO:19), E272K (SEQ ID NO:21), E289K (SEQ ID NO:23), K295E (SEQ ID NO:25) and D336R (SEQ ID NO:27).

The invention discloses a glucose response type insulin delivery system as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The glucose response type insulin delivery system provided by the invention comprises a delivery carrier and a bioactive component encapsulated in the carrier, the delivery carrier is a metal polyphenol network formed by carrying out coordination reaction on epigallocatechin gallate and metal ions; the bioactive components comprise glucose oxidase, catalase and insulin. The glucose response type insulin delivery system provided by the invention can adjust the release of insulin according to the concentration of glucose in the surrounding environment, so that the blood glucose is maintained at a normal level.

The invention relates to the technical field of image recognition, and discloses a carotid plaque stability and stroke risk prediction system fusing multiple modes, and the system comprises a data collection and fusion module, a feature extraction and association module, a risk assessment and layering module, an intervention decision module, and a report generation and feedback module. When carotid plaque stability and cerebral apoplexy risk prediction is carried out, plasma proteomics, radiomics and clinical data are synergistically integrated by establishing a multi-modal data fusion analysis framework, so that the limitation that a traditional method depends on a single data source is overcome; the plaque risk can be evaluated from multiple dimensions of biological activity and morphological features, the comprehensiveness and accuracy of risk prediction are improved, a more reliable diagnosis basis is provided for clinic, interaction and sensitivity influence among different modal features can be adaptively quantified by introducing dynamic feature correlation modeling and a real-time weight calibration mechanism, and the risk prediction accuracy is improved. And objectivity and consistency of risk assessment results are ensured.

The invention discloses a PBAT (poly (butylene adipate-co-terephthalate)) composite material containing nano encapsulation enzyme as well as a preparation method and application thereof. The PBAT composite material is prepared from the following components in percentage by mass: 50 to 90 parts of PBAT resin, 0 to 40 parts of filler and 0.01 to 20 parts of nano encapsulation enzyme. The preparation method comprises the following steps: firstly synthesizing the nano encapsulation enzyme, and then mixing the nano encapsulation enzyme, the PBAT resin and the filler, extruding, and carrying out hot press molding or curtain coating and film blowing. The method is characterized in that 1) the nano encapsulated enzyme has more excellent thermal stability than other immobilized enzymes, and the biological activity of the enzyme can be effectively maintained after high-temperature heat treatment; and 2) as the biological activity of the enzyme is effectively reserved, the PBAT composite material containing the nano encapsulated enzyme has higher environmental degradation speed than pure PBAT or a PBAT composite material containing free enzyme, and the environmental degradation speed can be reflected in various conditions such as aqueous solution and industrial compost.

The invention discloses an anti-degradation recombinant collagen as well as a preparation method and application thereof, and belongs to the technical field of collagen and bioengineering. According to the invention, full-length or partial genes of natural collagen in a human body are selected for certain optimization combination, a collagen amino acid sequence with strong stability and high biological activity is designed, and the amino acid sequence is completely consistent with a sequence of a corresponding region of the natural collagen in theory, and has relatively low immunogenicity; and the collagen has typical biological functions of human body collagen. The recombinant collagen prepared by the invention has excellent stability, biological activity and safety.

The invention discloses a milk-derived polypeptide sequence for promoting skeletal muscle cell proliferation and application of the milk-derived polypeptide sequence, and belongs to the field of bioactive peptides. The milk-derived bioactive polypeptide RHPHPHLSF provided by the invention has the effect of promoting muscle cell proliferation, and is expected to be used for preparing medicines for promoting muscle cell proliferation and preventing sarcopenia. The milk-derived bioactive polypeptide RHPHPHLSF is convenient to synthesize, can be industrially produced, and has a good application prospect in the fields of foods, medicines, cosmetics and the like.

The invention discloses a method for extracting extracellular vesicles from dried Sanguisorba officinalis rhizomes and application of the extracellular vesicles, and relates to the technical field of molecular biology and biomedicine.The method comprises the following steps that S1, rehydration is conducted, specifically, the dried Sanguisorba officinalis rhizomes are subjected to liquid nitrogen quick freezing and then ground into powder at the low temperature, the powder and hydration liquid are mixed according to the proportion of 1: 10 (w / v), the mixture is slowly stirred for 2-4 h at the temperature of 4 DEG C, and the extracellular vesicles are obtained; removing large-particle impurities by using sterile gauze or a filter screen, and collecting filtrate; according to the method, through the synergistic effect of multiple enzymolysis and in combination with cellulase, pectinase and the like, the degradation efficiency is remarkably improved, meanwhile, EDTA is chelated with metal ions, and the enzymolysis effect is enhanced. Short-time alternating ultraviolet light is introduced to activate'dormant 'vesicles, so that the yield and the biological activity are improved. Secondly, multiple protection mechanisms, such as vitamin C, EDTA (Ethylene Diamine Tetraacetic Acid), cane sugar, BSA (Bovine Serum Albumin) and an ultraviolet protective agent are adopted, so that the integrity and the biological activity of the vesicles are ensured.