175results about How to "Fold precisely" patented technology

Disclosed are methods and compositions for producing heterologous disulfide bond containing polypeptides in bacterial cells. In preferred embodiments the methods involve co-expression of a prokaryotic disulfide isomerase, such as DsbC or DsbG and a gene encoding a recombinant eukaryotic polypeptide. Exemplary polypeptides disclosed include tissue plasminogen activator.

Aggregation is a major cause of the misbehavior of proteins. A system for modifying a protein to create a more stable variant is provided. The method involves identifying non-conserved hydrophobic amino acid residues on the surface of a protein, suitable for mutating to more hydrophilic residues (e.g., charged amino acids). Any number of residues on the surface may be changed to create a variant that is more soluble, resistant to aggregation, has a greater ability to re-fold, and / or is more stable under a variety of conditions. The invention also provides GFP, streptavidin, and GST variants with an increased theoretical net charge created by the inventive technology. Kits are also provided for carrying out such modifications on any protein of interest.

The present invention relates to methods for selecting, obtaining or producing Fc variant polypeptides which show altered recognition for an Fc ligand (e.g., FcγR, CIq). Additionally, the Fc variant polypeptides may have altered antibody-dependent cell-mediated cytotoxicity (ADCC) and / or complement dependent cytotoxicity (CDC) activity. The invention further provides methods and protocols for the application of said Fc variant polypeptides particularly for therapeutic purposes.

A polypeptide self-antigen useful in a tumor-specific vaccine mimics one or more epitopes of an antigen uniquely expressed by cells of the tumor. The polypeptide is preferably produced in a plant that has been transformed or transfected with nucleic acid encoding the polypeptide and is obtainable from the plant in correctly folded, preferably soluble form without a need for denaturation and renaturation. This plant-produced polypeptide is immunogenic without a need for exogenous adjuvants or other immunostimulatory materials. The polypeptide is preferably an scFv molecule that bears the idiotype of the surface immunoglobulin of a non-Hodgkin's (or B cell) lymphoma. Upon administration to a subject with lymphoma, the plant-produced, tumor-unique scFv polypeptide induces an idiotype-specific antibody or cell-mediated immune response against the lymphoma.

A method and machine for folding and finishing training pant diapers, whereby the diapers are formed from respective flat blanks, each of which is fed to a continuously-rotating, suction-type first wheel, and is folded into a U by clamping an intermediate portion of the blank on the first wheel by means of a folding member; retaining a trailing half of the blank on the first wheel, while detaching a leading half from the first wheel by means of a continuously-rotating, suction-type second wheel; transferring the leading half to a continuously-rotating, suction-type third wheel; and gradually superimposing the leading half of the blank onto the trailing half, as the trailing half advances on the first wheel, and after first removing the folding member from the blank.

The invention relates to novel modified erythropoietin (EPO) forms such as fusion proteins comprising a Fc portion of an Ig molecule and a target molecule having the biological activity of EPO. By selective altering of the amino acid sequences of the erythropoietin moiety as well as of the immunoglobulin moiety and the glycosylation pattern of erythropoietin fusion proteins with enhanced biological activity can be obtained. The invention relates also to novel nun-fused EPO molecules which have a pattern of cysteines or disulfide bonding which is distinct from human or animal EPO.

The present invention provides a method for modifying a wild type nucleic acid sequence encoding a polypeptide to enhance expression and accumulation of the polypeptide in the host cell by harmonizing synonymous codon usage frequency between the foreign DNA and the host cell DNA. This can be done by substituting codons in the foreign coding sequence with codons of similar usage frequency from the host DNA / RNA which code for the same amino acid. The present invention also provides novel synthetic nucleic acid sequences prepared by the method of the invention.

Method and system for automatically forming packaging boxes. At least four folding units each provided with a controllable gripping element support a custom sized blank supporting an article in a supporting station. The at least one of the folding units is configured for folding respective side and / or end panels of the blank upwardly. The at least four folding units comprise a front pair of folding units and a rear pair of folding units positioned at a longitudinal distance from the front pair of folding units, and the folding units of the front pair and the folding units of the rear pair are positioned at a respective transverse distance from each other. Said longitudinal distance and / or said transverse distance are adjustable.

A method and machine for folding and finishing training pant diapers, whereby the diapers are formed from respective flat blanks, each of which is fed to a continuously-rotating, suction-type first wheel, and is folded into a U by clamping an intermediate portion of the blank on the first wheel by means of a folding member; retaining a trailing half of the blank on the first wheel, while detaching a leading half from the first wheel by means of a continuously-rotating, suction-type second wheel; transferring the leading half to a continuously-rotating, suction-type third wheel; and gradually superimposing the leading half of the blank onto the trailing half, as the trailing half advances on the first wheel, and after first removing the folding member from the blank.

Techniques for reorganizing security levels without implicating accessibility to secured files classified in accordance to one of the security levels are disclosed. In a case of adding a new security level, the controllability or restrictiveness of the new security level is determined with respect to the most restrictive security level or the least security level in a set of existing security levels. A set of proper security parameters are then generated for the new security level and subsequently the existing security levels are reorganized to accommodate the new security level. In a case of removing a security level from the existing security levels, the security parameters for the security level to be deleted are either folded up or down to an immediate next security level, depending on implementation. As a result, the security parameters for the immediate next security level are updated to include those for the security level to be deleted such that the secured files classified at the security level to be deleted can still be accessed by those with proper clearance levels.

The invention provides methods of increasing yields of desired conformation of proteins. In particular embodiments, the invention includes contacting preparations of a recombinant protein with a reduction / oxidation coupling reagent for a time sufficient to increase the relative proportion of a desired configurational isomer.

The present invention relates to proteome chips comprising arrays having a large proportion of all proteins expressed in a single species. The invention also relates to methods for making proteome chips. The invention also relates to methods for using proteome chips to systematically assay all protein interactions in a species in a high-throughput manner. The present invention also relates to methods for making and purifying eukaryotic proteins in a high-density array format. The invention also relates to methods for making protein arrays by attaching double-tagged fusion proteins to a solid support. The invention also relates to a method for identifying whether a signal is positive.

A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery, a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.

The invention belongs to the technical field of geometric measurement device verification devices, relates to a laser rangefinder verification device, and particularly relates to a laser rangefinder verification device based on the principle of error cancellation and a verification method thereof. The laser rangefinder verification device comprises a verification table. A fixing adjustment deviceis installed in the fixed end of the verification table. The fixing adjustment device is used for fixing a laser rangefinder to be verified. After the fixing adjustment device fixes a laser rangefinder to be verified, a self-walking target vehicle is installed in the verification table. A mobile reflection apparatus is installed in the self-walking target vehicle. A reflection target and at leastone fixed reflection apparatus are installed beside the fixing adjustment device at the fixed end of the verification table. A measurement reflector is installed at the rear end of the mobile reflection apparatus inside the self-walking target vehicle. A laser interferometer is installed at the end of the verification table away from the fixed end. The measurement reflector is used for reflectinga laser beam emitted by the laser interferometer.

Protein synthesis inhibitor resistance genes (typified by a cycloheximide resistance gene) are capable of imparting resistance to a protein synthesis inhibitor (typified by cycloheximide) to animal cells sensitive to the inhibitor. The genes have a sequence mutated by substitution in a gene encoding a ribosome-constituting protein derived from an animal. The genes may be placed in recombinant vectors, including expression vectors containing the gene together with a foreign protein structural gene.

Method and system for automatically forming packaging boxes. At least four folding units each provided with a controllable gripping element support a custom sized blank supporting an article in a supporting station. The at least one of the folding units is configured for folding respective side and / or end panels of the blank upwardly. The at least four folding units comprise a front pair of folding units and a rear pair of folding units positioned at a longitudinal distance from the front pair of folding units, and the folding units of the front pair and the folding units of the rear pair are positioned at a respective transverse distance from each other. Said longitudinal distance and / or said transverse distance are adjustable.

A system for expressing antigenic polypeptides in oligomeric form fuses the antigenic polypeptide to an oligomerisation polypeptide such that the oligomerisation polypeptide can interact with other oligomerisation polypeptides and bring multiple copies of the antigenic polypeptide into close proximity in the form of an oligomer. Expressing the polypeptides in oligomeric form in this way can improve their immunogenicity compared to a monomeric form.

In binding a cover sheet together with a sheet bundle, ever-accurate spine-creasing irrespective of bundle thickness is made possible. A sheet stacker stacks sheets into bundles, an adhesive applicator applies adhesive to a spine part of the sheet bundles, and a cover-sheet binder binds cover sheets together with the spine parts. The cover-sheet binder is constituted from a left-and-right pair of spine-creasing press members arranged free to shift between standby and spine-creasing positions; a shifter that reciprocates the spine-creasing press members between the standby and spine-creasing positions; and a controller. The controller functions to vary, in accordance with sheet bundle thickness, any of: (1) the standby position of the press members; (2) the start time of an operation whereby the press members are shifted from the standby to the spine-creasing position; or (3) the traveling speed of the press members in shifting from the standby to the spine-creasing position.

A machine for forming and ironing folds in cloth has a plate frame on which are fixed a support and pressure system, a template on which the cloth is put, a moving system for moving a blocking plate which blocks the cloth, a guide system for guiding a folding element which folds outer edges of the cloth and an ironing plate. The guide system has a guide profile and a translating arm which is translated in a longitudinal direction and has a roller and a folding element. The arm is under pressure towards the guide profile so that the roller beats and slides along the edge of the guide profile. In this way, the folding element follows the movement of the arm according to the guide profile and moves in the longitudinal direction and a transversal direction.

A collapsible ‘quasi 3D’ stereoscopic display device and method of construction for quick automatic deployment for use with a mobile smart device at any time and place and folding for storage within a mobile communication device (phone, tablet, portable computer) thin cover carrying case or other thin storage device.

A system for expressing antigenic polypeptides in oligomeric form fuses the antigenic polypeptide to an oligomerisation polypeptide such that the oligomerisation polypeptide can interact with other oligomerisation polypeptides and bring multiple copies of the antigenic polypeptide into close proximity in the form of an oligomer. Expressing the polypeptides in oligomeric form in this way can improve their immunogenicity compared to a monomeric form.

A bookbinding device includes a collecting device for collecting sheets into a bundle and a bookbinding path that includes a sheet bundle conveying device, an adhesive applying device, a cover binding device, and a cover sheet feeding path. The cover binding device includes a back folding press member for the cover sheet and a back rest plate member to back up the cover sheet. A control device allows the back folding press member to execute a back folding process with a small gap formed between the back rest plate member and a back of the sheet bundle. The control device further allows the back of the sheet bundle to abut against the back rest plate member to forcibly cool the adhesive. The control device varies the time required for the cooling depending on the thickness of the sheet bundle.

A shield shell (40) has a base (41) and a lid (42) formed together through a pair of coupling pieces (47). A housing (20) on which terminal members (21) are mounted is put on the base (41), and distal ends of shielded electric wires (11) are mounted on the housing (20). A core (12) in each shielded electric wire (11) is brought into insulation displacement in the terminal member (21), and a cover (35) closes the housing (20). The lid (42) is bent at the coupling pieces (47). Thus, the housing (20) is disposed and locked between the base (41) and the lid (42). Contact pieces (56) on the lid (42) of the shield shell (40) pass through windows (38) in the cover (38) and elastically contact a short circuit member (18).

The invention discloses a method for producing L-ornithine hydrochloride by TOP10 / PBAD / Thio-TOPO-Arg-Bsub colibacillus genetic engineering bacteria. The strain can express and generate excess bacillus subtilis arginase, and efficiently converts L-arginine into L-ornithine.

The invention relates to a recombinant adeno-associated virus. The recombinant adeno-associated virus is formed by inserting a tumor antigen gene into a shuttle expression vector pAAV-MCS. The invention also provides a construction method and an application of the recombinant adeno-associated virus. According to the invention, the recombinant adeno-associated virus carrying a tumor-associated antigen gene is utilized for infecting DC cells and expresses tumor-associated antigen protein in the DC cells, and a PD-1 gene is also combined for silencing CTL cells in the application method of the recombinant adeno-associated virus.

A storage organizing system comprises a basket and a pair of slide rail assemblies. A wrap is engaged about some of the surfaces of the basket. The wrap includes a plurality of panels each of which are configured to substantially cover a side of the basket. One or more of the panels include fixation mechanisms, such as ties, clips, clasps, buttons, snaps, hooks, hook and loop material, etc, to affix the wrap to the basket.