2316 results about "Analyte" patented technology

Glucose and ketones may be dysregulated singularly or concurrently in certain physiological conditions and may be advantageously assayed together using an analyte sensor capable of detecting both analytes. Certain analyte sensors capable of dual detection may comprise a first working electrode and a second working electrode, a ketones-responsive active area disposed upon a surface of the first working electrode, a glucose-responsive active area comprising a glucose-responsive enzyme disposed upon a surface of the second working electrode, a membrane having a first portion overcoating the ketones-responsive active area and a second portion overcoating the glucose-responsive active area, in which the first portion and the second portion have different compositions. The ketones-responsive active area comprises an enzyme system comprising at least two enzymes that are capable of acting in concert to facilitate detection of ketones.

Methods and analyte sensors including at least a first working electrode having a first active area thereon, and performing a dip coating operation to deposit a bilayer membrane upon the first working electrode and the first active area. The bilayer may include an inner layer having a first membrane polymer and an outer layer having a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another. The dip coating operation may comprise one or more first dips in a first membrane formulation to form the inner layer of the bilayer membrane and one or more second dips in a second membrane formulation to form the outer layer of the bilayer membrane upon the inner layer.

Methods and analyte sensors including at least a first working electrode having a first active area thereon, and performing a dip coating operation to deposit a bilayer membrane upon the first working electrode and the first active area. The bilayer may include an inner layer having a first membrane polymer and an outer layer having a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another. The dip coating operation may comprise one or more first dips in a first membrane formulation to form the inner layer of the bilayer membrane and one or more second dips in a second membrane formulation to form the outer layer of the bilayer membrane upon the inner layer.

Methods and analyte sensors including at least a first working electrode having a first active area thereon, and performing a dip coating operation to deposit a bilayer membrane upon the first working electrode and the first active area. The bilayer may include an inner layer having a first membrane polymer and an outer layer having a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another. The dip coating operation may comprise one or more first dips in a first membrane formulation to form the inner layer of the bilayer membrane and one or more second dips in a second membrane formulation to form the outer layer of the bilayer membrane upon the inner layer.

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

Different designs of an analyte sensor applicator for applying a wearable analyte monitoring device are disclosed. The analyte sensor applicator includes a housing that holds an analyte sensor assembly, a cam assembly for delivering the analyte sensor assembly, and a skin piercing for inserting a sensor, wherein the skin piercing device is retracted within the housing top after insertion.

This disclosure provides methods for spatial profiling of biological analytes present in a biological sample. Methods include generating feature arrays using a master / copy format using recessed arrays, and methods for using such arrays. For example spatially-tagged analyte capture analytes can be used in spatial detection in methods to determine the location of analytes (e.g., proteins) in biological samples.

Provided herein are methods of determining a location or presence of a target analyte in a biological sample by detecting oligonucleotide-conjugated binding agents.

Provided herein are methods for capturing an analyte from a first region of interest of a biological sample on a substrate, where the biological sample comprises the first region of interest and a second region, and where the method includes contacting the second region with a sealant in order to create a hydrophobic seal thereby preventing an interaction between an analyte from the second region with a capture domain of a capture probe.

The invention provides an electrochemical biosensor based on ultrasonic bidirectional driving and a detection method, and belongs to the technical field of biosensing. The sensor comprises a porous membrane and an ultrasonic bidirectional driving unit, and a probe capable of specifically capturing a target object is fixed in a pore channel of the porous membrane; the ultrasonic bidirectional driving unit is composed of a first ultrasonic transducer and a second ultrasonic transducer which are symmetrically arranged on the two sides of the porous membrane, and can synchronously execute reverse power gradient change to form alternately changing pressure difference on the two sides of the membrane so as to drive liquid to repeatedly penetrate through the porous membrane. The detection method comprises the steps of to-be-detected liquid injection and ultrasonic bidirectional driving, ultrasonic auxiliary cleaning and electrochemical detection. The problems of insufficient probe utilization rate, low mass transfer efficiency and non-specific adsorption of a traditional passive diffusion mechanism are solved by utilizing bidirectional ultrasonic active driving force, the detection sensitivity, speed and signal-to-noise ratio are remarkably improved, and the method is suitable for rapid and high-sensitivity quantitative analysis of various analytes.

The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.

The present embodiments relate generally to systems and methods for measuring an analyte in a host. More particularly, the present embodiments provide sensor applicators and methods of use to insert the sensor into an individual's skin. Applicators are disclosed for inserting the sensor. Such applicators may be reusable applicators configured to implant multiple different sensors.

The present invention relates to modified Dda helicases which can be used to control the movement of analytes such as polynucleotides. The modified Dda helicases are used in analyte detection and characterisation. The present invention also relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore.

An evaporation device includes a sample fluid channel extending from a sample fluid inlet to a sample fluid outlet. The evaporation device is configured to evaporate a solvent of a sample fluid containing a target analyte to increase the concentration of the target analyte. The device includes a housing enclosing and defining a testing environment within which the evaporation device is disposed. The housing includes a heater, a temperature sensor, and a humidity sensor. A pump coupled to a gas inlet tube is configured to cause a gas flow within the testing environment adjacent to the evaporation device. A controller in communication with each of the heater, the temperature sensor, and the humidity sensor, is configured to activate the heater in response to at least one of a temperature value or a humidity value of the testing environment.

An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.

Systems, devices and methods are provided for determining a medication dose for a patient or user. The dose determination can account for recent and / or historical analyte levels of the patient or user. The dose determination can also take into account other information about the patient or user, such as physiological information, dietary information, activity, and / or behavior. Many different dose determination embodiments are set forth, pertaining to a wide array of different aspects of the system or environment in which the embodiments can be implemented.

Providing a fluid to an instrument comprises supporting a sample receptacle on a first carrier, transporting the first carrier on a conveyor adjacent a plurality of modules including at least one instrument, stopping the first carrier at the instrument, transporting the sample receptacle to a pick-up position of the instrument, transporting the sample receptacle from the pick-up position to a pipetting station within the instrument, aspirating fluid within the sample receptacle and transferring the aspirated fluid to a reaction receptacle, after aspirating fluid from the sample receptacle, transporting the sample receptacle to the pick-up position, transporting the sample receptacle from the pick-up position to a second carrier the conveyor, in the instrument, performing an assay to determine the presence or absence of an analyte in the aspirated fluid, and transporting the second carrier on the conveyor to one or more of the plurality of modules other than the analytical instrument.

Provided herein are methods, compositions, and kits for detecting analytes of interest from biological samples using tagmentation. The method comprises: (a) hybridising a nucleic acid analyte to a capture probe on an array, wherein the capture probe comprises: (i) a spatial barcode and (ii) a capture domain; (b) extending the capture probe using the nucleic acid analyte as a template, thereby generating a barcoded nucleic acid molecule on the array, wherein the barcoded nucleic acid molecule comprises a complement of the sequence of the nucleic acid analyte; and (c) tagmenting the barcoded nucleic acid molecule or a derivative thereof using a transposome comprising a transposase to insert a transposon end sequence into the barcoded nucleic acid molecule or the derivative thereof, thereby generating a barcoded nucleic acid fragment, wherein the barcoded nucleic acid fragment comprises: (i) the barcode, (ii) the transposon end sequence, and (iii) at least a portion of a sequence of the nucleic acid analyte or a complement thereof.

A computer-implemented method is provided for monitoring and / or controlling a chemical and / or biological process. The method comprises: obtaining (S10) a time series dataset comprising observations, each one of the observations being collected with respect to the chemical and / or biological process at a particular time point, wherein each one of the observations includes values of observed parameters obtained with a spectroscopic method at the particular time point and a value of an analyte parameter obtained with a reference measurement method at the particular time point; obtaining (S20) a prediction model for estimating a predicted value of the analyte parameter in the chemical and / or biological process, the prediction model being trained using at least part of the time series dataset; validating (S30) the prediction model by assessing an ability of the prediction model to predict a difference between an actual value of the analyte parameter which would be obtained with the reference measurement method at a given time point and an expected value of the analyte parameter at the given time point, using a set of observations that is not included in the at least part of the time series dataset used for training the prediction model; determining (S40) whether the prediction model is valid or invalid based on a result of the validating; and monitoring and / or controlling (S50) the chemical and / or biological process when the prediction model is determined to be valid.

A point of use analyte detection and quantification system for chemical applications is provided. Related methods are also provided.

A method for correcting a data set for a target analyte in a sample, according to a first embodiment, comprises the steps of: obtaining a data set for a target analyte; generating an amplification function including the data set; generating a derivative by differentiating the amplification function, wherein the derivative includes at least one peak and a local maximum point located on the peak; selecting a first local maximum point having the smallest cycle number among the at least one local maximum point; determining an amplification recognition cycle by using the first local maximum point; setting a baseline fitted to a data set corresponding to a cycle equal to or less than the amplification recognition cycle; and correcting the data set by subtracting the baseline from the data set.

This disclosure relates to magnetic microgel beads, and in particular to magnetic microgel beads for biofunctionalization and methods of making and uses thereof, for example, in biosensing assays. In an embodiment, a magnetic microparticle comprising a magnetic nanoparticle encapsulated by a polymer hydrogel. In another embodiment, an assay for detecting the presence of a target analyte in a sample comprising a) the magnetic microparticle disclosed herein, wherein the biorecognition agent further comprises a reporter moiety; b) an electrochemical chip comprising a working electrode, a counter electrode and a reference electrode; and c) a capture probe functionalized on the working electrode; wherein binding of the biorecognition agent to the target analyte results in production of an electrochemical, electroluminescent or photoelectrochemical signal.

Provided herein are kits, systems and methods for analyzing analytes from a biological sample. The system includes an upper housing configured to receive a sample substrate and a thermal element configured to control a temperature of the sample substrate. The system includes a lower housing and an alignment mechanism coupling the upper housing to the lower housing. The alignment mechanism is configured to move the upper housing, the lower housing, or both into an arrangement having an open configuration, a closed configuration, or an intermediate configuration therebetween. The thermal element is configured to cool the sample substrate.

The invention discloses an information processing method, device and equipment for analyte concentration monitoring and a medium, and relates to the technical field of physiological signal analysis, applied to a computer device.The method comprises the steps that an original analyte response signal set generated by an analyte sensor based on a reference physiological fluid is obtained, the signal group comprises response sensitivity, a base line and response current; performing temperature compensation on the signal group according to the current temperature fed back by the temperature sensor, and determining the concentration value of the target analyte in the reference physiological fluid by using the compensated signal group; substituting the concentration value of the target analyte in the reference physiological fluid into a target model to obtain the concentration of the target analyte in the target physiological fluid; the target model is constructed on the basis of a regulation and control mechanism of the temperature to the transport relation of the analyte and a regulation and control mechanism of the temperature to the metabolic relation of the analyte, and the concentration of the target analyte in the target physiological fluid is determined as a concentration monitoring result. By means of the scheme, the accuracy of analyte concentration monitoring is improved.

Systems and methods for concurrent multi-frequency impedance measurements to characterize a substance of interest (e.g., analyte concentration) are disclosed herein. Generally, in some variations a method for characterizing a substance includes generating an input signal having a first set of frequency components across a plurality of frequencies, exciting an electrode arrangement with the input signal wherein the electrode arrangement is in contact with the substance. receiving an output signal from the excited electrode arrangement, the output signal having a second set of frequency components across the plurality of frequencies, determining an impedance signature of the substance across the plurality of frequencies, based on the first and second sets of frequency components, and characterizing the substance based on the impedance signature.

Provided herein are methods, kits and compositions for detecting an analyte of interest to interrogate spatial gene expression in a sample using templated ligation.

The utility model relates to transmitting equipment for medical monitoring, and discloses an applicator for transmitting an analyte sensor, which comprises a needle assisting shell, a trigger sleeve and a boosting core, the needle assisting shell is sleeved on the trigger sleeve, the boosting core is accommodated in the trigger sleeve through a locking mechanism, and an elastic element is arranged between the trigger sleeve and the boosting core; the locking mechanism comprises an ejector block arranged on the inner wall of the needle assisting shell, a limiting elastic piece arranged on the trigger sleeve and a limiting hole formed in the boosting core. During locking, the ejector block abuts against the limiting elastic piece, and the lower end of the limiting elastic piece is limited in the limiting hole. During unlocking, the needle assisting shell drives the ejector block to be separated from the limiting elastic piece, and the elastic element drives the boosting core to eject the limiting elastic piece open. The locking mechanism can be unlocked by exerting acting force on the auxiliary needle shell, the auxiliary needle shell pushes the boosting core to move downwards, the ejector block on the auxiliary needle shell is separated from the limiting elastic piece, the locking mechanism is unlocked, the boosting core is released, no additional component is needed to drive the boosting core, and no window needs to be formed in the auxiliary needle shell.