183 results about "Concentrations glucose" patented technology

The invention relates to the technical field of biomedical sensing and health monitoring, and discloses a noninvasive glucose detection system. The system comprises an interstitial fluid extraction device, a photoelectric sensing module and a main control circuit which are integrated on a flexible substrate. The interstitial fluid extraction device drives subcutaneous interstitial fluid to migrate to the hydrogel interface layer under the action of reverse ion electroosmosis; the photoelectric sensing module receives interstitial fluid through a hydrogel interface layer, a working electrode of the photoelectric sensing module comprises a Cu monatomic imprinting recognition layer, and imprinting holes formed by Cu monatomic sites are specifically coordinated with glucose molecules. The master control circuit controls sampling and determines the glucose concentration based on the photocurrent signal. Noninvasive sampling and monatomic imprinting photoelectric detection are fused, sampling-detection integration is achieved, and the device has the advantages of being noninvasive, painless, high in selectivity, high in interference resistance, comfortable to wear and the like and is suitable for continuous monitoring of blood glucose.

The invention provides schizochytrium limacinum FJ-1 and application thereof in production of phospholipid type EPA (eicosapentaenoic acid). The application comprises the following steps: taking a cryopreserved schizochytrium limacinum strain, and inoculating the schizochytrium limacinum strain into a liquid seed culture medium for pre-culture; the method comprises the following steps: inoculating pre-cultured schizochytrium limacinum into a fermentation tank according to the inoculum size of 5-10% v / v, culturing for 48-60 hours at the sea salt concentration of 0.5-1% w / v, fermenting for 48-72 hours, supplementing sea salt to a culture medium in batches until the sea salt concentration is 3-5% w / v, and continuously culturing for 96-120 hours by controlling the glucose concentration and the yeast extract concentration through supplementing materials. The technical problem that the synthesis efficiency of schizochytrium limacinum phospholipid type EPA is low is solved through precise regulation and control of salinity stress, an efficient, economical and generalizable innovative scheme is provided for industrial production of functional lipid, and the method is suitable for large-scale production.

The invention relates to the crossing field of computer technology and biomedical engineering, and discloses a miniaturized intelligent blood glucose monitoring and analyzing method integrating edge computing. The method comprises the steps that glucose concentration and time sequence multi-source physiological signals are collected through a wearable sensor; performing data segmentation and multi-modal feature alignment based on the circadian rhythm; inputting the features into a lightweight student model obtained through knowledge distillation for blood glucose prediction; and the reasoning frequency is adaptively adjusted in combination with a memory feedback mechanism. The system comprises a sensing unit, a preprocessing unit, a lightweight model execution unit and a risk early warning unit. While the prediction precision is guaranteed, the model is compressed to be within 150 thousand parameters, a 512 KB on-chip memory is adapted, and low-power-consumption and high-stability end-side continuous blood glucose monitoring and early warning are achieved.

The invention discloses saccharomyces cerevisiae recombinant bacteria for efficiently producing terpenoids as well as a construction method and application of the saccharomyces cerevisiae recombinant bacteria. According to the construction method, saccharomyces cerevisiae rich in terpenoid precursor farnesyl pyrophosphate is taken as a starting strain, the GAL80 protein level in a galactose induction system is regulated and controlled by introducing an N-Degron strategy, and meanwhile, a glucose concentration response type promoter HXT1 is introduced to effectively regulate and control the expression of GAL80 from the transcriptional level; the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids can be obtained. The strategy is applied to a saccharomyces cerevisiae engineering strain of beta-elemene and patchouli alcohol, and a remarkable effect is achieved. And in the high-density fermentation process of the saccharomyces cerevisiae, the yield of the beta-elemene is obviously increased and reaches 4818.6 mg / L. The invention provides a solid research basis for sustainable biosynthesis of terpenoids, and has a wide application prospect.

The invention provides a DNA enzyme triple-helix molecular switch detection system adaptive to a personal glucometer as well as a preparation method and application of the DNA enzyme triple-helix molecular switch detection system, and belongs to the technical field of biosensors. The DNA enzyme triple-helix molecular switch disclosed by the invention comprises an aptamer containing two nucleotide arm segments and DNA enzyme covalently coupled with a magnetic bead, two nucleotide arm segments of the aptamer and DNA enzyme form a triple helix structure; the two nucleotide arm segments comprise a nucleotide sequence SEQ ID NO.1 at the 5'end of the aptamer and a nucleotide sequence SEQ ID NO.2 at the 3 'end of the aptamer. According to the invention, the flexibility of an original aptamer is limited by using a terminal fixation strategy, the spatial conformation of the original aptamer is stabilized, the affinity of the aptamer to a target is improved, and the sensitivity of the aptamer to the target is improved by using a triple-helix molecular switch containing DNA enzyme and nano-enzyme-gold nanoparticles. Accurate and sensitive detection of trace pollutants in food is realized by measuring the reduction amplitude of the glucose concentration in the detection liquid through a household glucometer.

A method may comprise (a) inserting an insulin delivery device subcutaneously into a body of a subject, which comprises an amperometric glucose sensor comprising an electrode layer comprising an indicating electrode and underlying a redox-catalytic layer comprising a redox mediator; (b) using said insulin delivery device to deliver an insulin formulation (e.g., comprising a phenol or cresol excipient) subcutaneously to said subject; and (c) using said amperometric glucose sensor to measure a subcutaneous glucose concentration, which comprises using said redox-catalytic layer to allow electron transfer from subcutaneous glucose to said indicating electrode sufficient to cause a response of said amperometric glucose sensor at an applied bias potential of no more than +250 millivolts, wherein (b) and (c) are performed at the same time for at least one hour, while maintaining a sensor sensitivity of at least 50% of an initial sensor sensitivity for up to one hour.

In an aspect, the present disclosure provides a method comprising: (a) positioning an insulin delivery device on a body of a subject via a single puncture site in said body of said subject; (b) using said insulin delivery device to deliver an insulin or insulin analog formulation to said subject via said single puncture site; and (c) using a sensor of said insulin delivery device to measure a glucose concentration in said body of said subject via said single puncture site, wherein (b) and (c) are performed at substantially the same time for a time period of at least 1 hour subsequent to said positioning in (a), while maintaining a sensitivity of said sensor of at least 50% of an initial sensitivity of said sensor.

The invention provides a calibration method for real-time dynamic blood glucose monitoring. The calibration method comprises the steps that 1, weak current signals generated by a glucose sensitive electrode are obtained regularly; 2, filtering the detected weak current signal by adopting a sliding window double-layer screening algorithm; 3, monitoring the body temperature of the human body, compensating the temperature based on the filtered weak current value, and estimating the glucose concentration after temperature compensation; 4, monitoring the PH value of the human blood, compensating the PH value according to the filtered weak current value, and estimating the glucose concentration after PH value compensation; and 5, combining the glucose concentrations after temperature and pH value compensation, and carrying out filtering processing on the combined glucose concentrations by adopting a parameter compensation filtering algorithm to obtain a final glucose concentration estimation result.

The invention relates to the technical field of medical detection equipment, and particularly provides a wearable sweat glucose detection system which comprises a sweat collecting and conveying unit which is provided with a sweat inlet and a sweat flow channel which are communicated with each other and is used for collecting and conveying sweat secreted by skin; the electric signal acquisition unit is arranged on the sweat flow channel, comprises a counter electrode, a working electrode and a reference electrode which are sequentially arranged along the flowing direction of sweat in the sweat flow channel, and is used for acquiring a real-time electric signal related to the glucose concentration; the working electrode comprises a porous alloy structure layer composed of platinum and nickel and a glucose oxidase layer located on the surface of the porous alloy structure layer; the signal processing unit is used for acquiring the real-time glucose concentration according to the real-time electric signal and a preset change curve of the electric signal about the glucose concentration; the system can effectively improve the sensitivity of glucose concentration detection and reduce the dosage of precious metal.

The invention relates to a system for storing or cultivating organ or tissue models and its use. The system is designed for verifying the functionality of the harvested organ or tissue models and allowing them to be processed. The system can be used for cultivating organ and / or tissue models harvested from donors and / or prepared using other techniques, such as using 3D and / or 4D bioprinting or electrospinning techniques, and comprises a chamber (2) for the organ or tissue models, which is equipped with means for measuring and adjusting the temperature of the perfusion liquid in the chamber, a perfusion liquid container (1), which is equipped with means for measuring and adjusting the temperature of the perfusion liquid therein, means (4) for measuring the glucose concentration in the perfusion liquid and adding glucose to the perfusion liquid in a metered amount, means (3) for measuring the pH of the perfusion liquid and adding a substance for adjusting the pH of the perfusion liquid in a metered amount, means for non-contact mixing of the perfusion liquid, and means for measuring and controlling the flow parameters of the perfusion liquid.

The invention discloses a corneal glucose real-time non-invasive detection system and method, and belongs to the technical field of glucose real-time non-invasive detection.The method comprises the following steps that corneal reflection spectrum, tear film thickness change and environment temperature and humidity data are obtained in real time, and spectrum compensation parameters are generated through a temperature and humidity compensation model; outputting a tear film refractive index correction value in combination with a refractive index correction model, and correcting the reflection spectrum through a combined correction formula; then, calculating a reference reflection spectrum and a deviation value based on historical data, extracting statistical characteristics, and fusing confidence to obtain a confidence factor for adjusting the deviation value; if the deviation exceeds the threshold value, replacing the corrected data with the reference data; and finally, calculating the glucose concentration according to the processed spectrum, and determining the health grade according to a preset comparison table. The high-reliability non-invasive blood glucose health monitoring closed-loop method and system are constructed through multi-source data fusion correction, intelligent deviation optimization and a confidence coefficient decision mechanism.

The invention relates to a method for monitoring host physical sign parameters on line and a multi-working electrode system. The accuracy of glucose monitoring can be improved. The method comprises the following steps: acquiring a first physical sign parameter of a host by adopting a first working electrode; acquiring a second physical sign parameter of the host by adopting a second working electrode; obtaining a target glucose concentration of the host based on the first physical sign parameter and the second physical sign parameter; the first working electrode and the second working electrode are arranged on the same substrate and can independently obtain a first physical sign parameter and a second physical sign parameter at different depths of the host tissue. The two working electrodes can effectively detect the concentrations of analytes at different depths, and by combining the sign parameters generated by the different working electrodes, a more reliable continuous monitoring result can be comprehensively obtained, and the monitoring accuracy is improved.

The invention provides a glucose hydrogel optical fiber sensor and a preparation method thereof. The glucose hydrogel optical fiber sensor comprises an optical fiber and a glucose fluorescence sensing module fixedly carried on the end face of the optical fiber. Wherein the optical fiber comprises a double-layer hydrogel optical fiber which comprises an inner fiber core and an outer cladding. The glucose fluorescence sensing module is formed by fixing a glucose fluorescence probe containing a boric acid group and a terminal amino group through a covalent reaction between the amino group and an epoxy group in the hydrogel network. The glucose hydrogel optical fiber sensor has excellent performance, high sensitivity and good selectivity, and can realize stable and real-time detection of glucose concentration in a complex physiological environment. The sensor is flexible in structure and good in biocompatibility, does not depend on enzyme reaction, and avoids the problem of signal drift caused by enzyme inactivation; meanwhile, the sensor is good in repeatability, can be integrated with other optical sensing modules to realize multi-parameter synchronous monitoring, and is suitable for long-term dynamic monitoring application in vivo.

Provided in the present application are a blood glucose monitoring method and a related apparatus. In the method, the blood glucose level at a first moment can be acquired on the basis of a glucose concentration in interstitial fluid at the first moment, a glucose concentration in the interstitial fluid within a time period before the first moment and a glucose concentration in the interstitial fluid within a time period after the first moment. In the present application, the glucose concentration in the interstitial fluid within the time period after the first moment is taken into consideration, and an additional time period is provided for the diffusion of glucose between the interstitial fluid and blood; therefore, when the blood glucose level drastically changes, the glucose concentration in the interstitial fluid more closely approximates the blood glucose level, and thus the accuracy of blood glucose monitoring can be improved.

A sensor device is adapted to detect glucose concentration in saliva of an object, and includes a conductive substrate, a polymer layer, and a carbon nanomaterial unit. The polymer layer is disposed on the conductive substrate and is made from conducting polymer. The carbon nanomaterial unit is disposed on the polymer layer and includes at least one carbon-nanotube layer. The saliva of the object is applied on the carbon nanomaterial unit and an enzyme is added to be mixed with the saliva to detect the glucose concentration in the saliva. A method for making the sensor device is also provided.

The invention discloses a BHK-21 cell high-density multiplication culture device and method, and relates to the technical field of cell high-density multiplication culture, the BHK-21 cell high-density multiplication culture device comprises a lower cover, the edge of one end of the lower cover is rotatably connected with an upper cover, the bottom of the inner wall of the lower cover is fixedly connected with a fixed shell, and the bottom of the inner wall of the fixed shell is provided with a plurality of culture boxes; a second handle and a fixing piece are fixedly connected to the edge of the other end of the lower cover, a first handle is fixedly connected to the edge of one end of the upper cover, and a rotating piece is rotationally connected to the position, close to the first handle, of the edge of one end of the upper cover. According to the BHK-21 cell high-density multiplication culture device and method disclosed by the invention, a sample with the maximum cell number and the most stable PH value is found out through mutual contrast of multiple groups of experiments, the glucose concentration for most efficiently culturing BHK-21 strain cells is obtained, and meanwhile, the utilization efficiency of a culture solution is improved by using a suspension mechanism; therefore, the effect of improving the culture density of the BHK-21 strain cells is achieved.

This invention discloses a green synthesis method for acid-base bifunctional molecular sieves and its application. The method constructs acid-base sites in a one-step synthesis, yielding a molecular sieve catalyst containing acid-base bifunctional sites. This molecular sieve exhibits high activity and stable performance, and can be used as a catalyst to catalyze the isomerization of glucose to fructose. It significantly improves conversion efficiency in reactions catalyzing the isomerization of high-concentration glucose to fructose or its conversion to methyl lactate. Furthermore, this synthesis method avoids the use of environmentally harmful fluorinated mineralizers and inorganic acids, solving the problems of complex, time-consuming, and environmentally unfriendly synthesis processes of existing acid-base bifunctional molecular sieves. It also addresses the issues of low reaction efficiency and low selectivity of existing catalysts in reactions catalyzing the conversion of high-concentration glucose.

A method of determining glucose values during continuous glucose monitoring (CGM) measurements includes providing a CGM device including a sensor, a memory, and a processor; applying a constant voltage potential to the sensor; measuring a primary current signal resulting from the constant voltage potential and storing the measured primary current signal in the memory; applying a probe potential modulation sequence to the sensor; measuring a probe potential modulation current signal resulting from the probe potential modulation sequence and storing the measured probe potential modulation current signal in the memory; determining an initial glucose concentration based on a conversion function and the measured probe potential modulation current signal; determining a connection function value based on the primary current signal and a plurality of the probe potential modulation current signals; and determining a final glucose concentration based on the initial glucose concentration and the connection function value. Other aspects are disclosed.

The application belongs to the field of microorganisms, and particularly relates to a strain of ultrahigh molecular weight gamma-polyglutamic acid synthesis strain and application thereof. The specific technical scheme is as follows: a strain of Bacillus subtilis FB-3 is preserved in the China General Microbiological Culture Collection Center on April 29, 2022, and the preservation number is CGMCC NO. 24826. The Bacillus subtilis is inoculated into a gamma-PGA fermentation medium, and is subjected to early fermentation under the conditions of a glucose concentration of 5-20 g / L and a ventilation amount of 0.2-3 vvm for 6-18 hours. After the early fermentation is completed, the oxygen supply level in the fermentation system is adjusted in real time, and qO2 is maintained constant at 15-40 mmol / (g.h). The application provides a new strain of Bacillus subtilis, which can produce gamma-PGA with a molecular weight of up to 25000 KDa, far exceeding the existing level of the same kind of microorganism. The method provided by the application is simple to operate, can be automatically monitored and calculated, has low requirements on operators, is suitable for large-scale fermentation production of ultrahigh molecular weight gamma-PGA, and has industrial popularization and application value.

A method may comprise (a) inserting an insulin delivery device subcutaneously into a body of a subject, which comprises an amperometric glucose sensor comprising an electrode layer comprising an indicating electrode and underlying a redox-catalytic layer comprising a redox mediator; (b) using said insulin delivery device to deliver an insulin formulation (e.g., comprising a phenol or cresol excipient) subcutaneously to said subject; and (c) using said amperometric glucose sensor to measure a subcutaneous glucose concentration, which comprises using said redox-catalytic layer to allow electron transfer from subcutaneous glucose to said indicating electrode sufficient to cause a response of said amperometric glucose sensor at an applied bias potential of no more than +250 millivolts, wherein (b) and (c) are performed at the same time for at least one hour, while maintaining a sensor sensitivity of at least 50% of an initial sensor sensitivity for up to one hour.

The present invention belongs to the technical field of insulin derivatives. Disclosed are a phenylboronic-acid-based insulin derivative, and a preparation method therefor and the use thereof. The provided phenylboronic-acid-based insulin derivative has a good glucose-responsive release function, wherein the phenylboronic acid group binds to a glycosyl protein to generate a phenylboronic acid ester bond, thereby generating an insulin and glycosyl protein complex in situ in blood. When the concentration of glucose in the blood is high, the glucose specifically cleaves the phenylboronic acid ester bond, thereby releasing the insulin. The provided phenylboronic-acid-based insulin derivative achieves stable and rapid glucose-responsive insulin release, which can steadily and persistently maintain the blood glucose of a diabetic subject within the normal range with almost no occurrence of hypoglycemia.

Methods and apparatus, including computer program products, are provided for processing analyte data. In some example implementations, a method may include generating glucose sensor data indicative of a host's glucose concentration using a glucose sensor; calculating a glycemic variability index (GVI) value based on the glucose sensor data; and providing output to a user responsive to the calculated glycemic variability index value. The GVI may be a ratio of a length of a line representative of the sensor data and an ideal length of the line. Related systems, methods, and articles of manufacture are also disclosed.

An agent is for inhibiting glucose uptake into red blood cells and a blood collection tube suppresses glucose concentration decrease. The agent for inhibiting glucose uptake into red blood cells includes inosine as an active ingredient. The inhibition agent can be used for suppressing a decrease in glucose concentration of whole blood collected in a blood collection tube. The blood collection tube includes inosine in an internal space. The blood collection tube can be used to measure glucose concentration in blood. The blood collection tube can further include a glycolysis inhibitor.

The invention relates to a method for improving PD-1 titer produced by Chinese hamster ovary cells. The method comprises the following steps: inoculating the Chinese hamster ovary cells into an initial culture medium for culturing; after the culture is performed for 48 hours, feeding a fed-batch culture medium every day, and maintaining the glucose concentration in the culture medium to be 1.3-1.7 g / L, the galactose concentration to be 0.6-1.0 g / L and the L-sodium lactate concentration to be 14-16 mM; the culture temperature is reduced, a fed-batch culture medium is fed every day, the glucose concentration in the culture medium is maintained at (2-2.8) g / L, the galactose concentration is maintained at (1.7-2.2) g / L, and cells are harvested. Galactose is introduced to serve as a second carbon source, and control over the culture initial metabolism environment and the carbon source in the culture stage is combined, so that the maximum accumulation amount of lactic acid is obviously reduced, the cell culture period is remarkably prolonged, and the maximum titer of the PD-1 antibody is remarkably improved compared with an existing process.

A glucose concentration estimation device includes a first light source, a second light source, a third light source, a light receiving element, and an estimation unit. The first light source irradiates a living body with first light including light of one wavelength in the wavelength range of 1375-1395 nm. The second light source irradiates the living body with second light including light of one wavelength in the wavelength range of 1575-1595 nm. The third light source irradiates the living body with third light including light of one wavelength in the wavelength range of 1835-1855 nm. The light receiving element receives reflected light returning from the living body when the living body is irradiated with the first to third light. The estimation unit estimates the glucose concentration on the basis of the output of the light-receiving element.

The present invention relates to methods for preparing hyaluronidase and variants thereof. Specifically, the methods are capable of altering N-glycan levels under culture conditions, including controlled glucose concentration within the culture medium and reduced culture temperature and specified culture time, thereby increasing specific activity by 10% or more, and improving quality and yield.

The invention discloses a method for improving clostridium anaerobic fermentation hydrogen volume yield and hydrogen production rate and application. According to the method, biochar is added into a dark fermentation culture medium for anaerobic fermentation to obtain hydrogen. According to the method, the clostridium can be adsorbed on the surface and inside the biochar, so that the clostridium is prevented from being directly contacted with a high-concentration glucose-containing culture medium to a certain extent, the inhibition effect of high-concentration glucose is reduced, and the hydrogen production rate and the glucose utilization rate are increased. Moreover, the biochar is alkaline after being dissolved in water, so that the rapid reduction of the pH of the culture system can be relieved. Meanwhile, the porosity of the biochar is also developed, and the biochar can adsorb part of organic acids such as acetic acid and butyric acid generated by glucose metabolism, so that the inhibition effect of the organic acids on hydrogen production is relieved, and the hydrogen production rate and the glucose utilization rate are further improved. In addition, the method also has the characteristics of low cost, simplicity and convenience in operation and the like, and large-scale industrial application is easy to realize.

A method for measuring glucose and cortisol levels in earwax, wherein the measured cortisol and glucose levels are interpreted as average cortisol and glucose levels, and a medical device that provides effective, reliable, safe 5 and hygienic self-extraction of earwax.

The invention relates to the field of medicine, and more particularly to biosensor analytical devices and can be used for quantifying the concentration of glucose in blood, plasma and serum. The present device comprises a printed graphite electrode, the surface of which is modified with a hybrid composite material incorporating a biorecognition element in the form of glucose oxidase, wherein the hybrid composite material is a redox-active polymer based on chitosan covalently bonded to a mediator in the form of Nile blue. The technical result is that of broadening the range of detectable glucose concentrations to 0.006-1.0 мМ.

The application provides a DNA enzyme triple helix molecular switch detection system suitable for a personal blood glucose meter, and a preparation method and application thereof, and belongs to the technical field of biosensors.The DNA enzyme triple helix molecular switch comprises an aptamer containing two nucleotide arm segments and DNA enzyme covalently coupled with a magnetic bead; the two nucleotide arm segments of the aptamer form a triple helix structure with the DNA enzyme; and the two nucleotide arm segments comprise a 5' end nucleotide sequence SEQ ID NO.1 of the aptamer and a 3' end nucleotide sequence SEQ ID NO.2 of the aptamer.The application uses a terminal fixation strategy to limit the flexibility of the original aptamer, stabilize the spatial conformation thereof, improve the affinity of the aptamer to the target, utilize the triple helix molecular switch containing the DNA enzyme and the nano-enzyme-gold nanoparticles, and realize accurate and sensitive detection of trace pollutants in food through determination of the reduction range of the glucose concentration in the detection liquid by using the household blood glucose meter.