288 results about "Concentrations glucose" patented technology

The invention provides a virtual-real fusion method for digital twinning operation of a cell drug factory in combination with the Internet of Things, which comprises the following steps: constructing geometric models of a culture medium storage tank and a bioreactor through three-dimensional modeling software, mapping and associating historical process parameters of a culture medium supply system and temperature, pH value and dissolved oxygen concentration monitored in real time with the geometric models, and obtaining a virtual-real fusion model of the culture medium storage tank and the bioreactor; constructing a digital twinborn model of the reactor; analyzing historical production data through a machine learning algorithm, extracting a quantitative relationship among key parameters of a cell metabolism rate, a glucose concentration in a culture medium, a pH value and a reactor temperature, establishing a cell growth prediction model, and predicting a growth trend and a metabolism demand of cells in a target time period; a virtual-real mapping mechanism of a culture medium supply system is integrated with an intelligent manufacturing execution system, so that the culture medium supply process and the whole pharmaceutical production process are subjected to collaborative optimization control, and the working procedures of the culture medium production process are automatically dispatched according to a production plan and cell growth requirements.

The invention discloses a preparation method of an MOF glucose detection system, ZIF is used as a metal organic framework, glucose oxidase (GOx) is loaded in the ZIF, the preparation method comprises the following steps: S1, water is added into a reaction bottle in advance, then metal ions, enzyme and organic ligands with the molar ratio of (100-1500): (1-10): (1-150) are added into the reaction bottle in advance, and the reaction bottle is filled with water; the method comprises the following steps: S1, adding 0.1-0.5% (by weight) of ammonia water into a reaction bottle, S2, mixing and reacting at room temperature for more than 6 hours, and S3, centrifuging by a centrifugal machine, and removing supernate to obtain solid precipitate of the GOx-MOF material loaded with glucose oxidase. The invention also discloses an application of the MOF glucose detection system in saliva glucose content detection. The method can be applied to glucose detection of oral saliva, and even if the glucose content is extremely low, the glucose concentration in the saliva can be efficiently and accurately detected.

The invention relates to the technical field of biomedical sensing and health monitoring, and discloses a noninvasive glucose detection system. The system comprises an interstitial fluid extraction device, a photoelectric sensing module and a main control circuit which are integrated on a flexible substrate. The interstitial fluid extraction device drives subcutaneous interstitial fluid to migrate to the hydrogel interface layer under the action of reverse ion electroosmosis; the photoelectric sensing module receives interstitial fluid through a hydrogel interface layer, a working electrode of the photoelectric sensing module comprises a Cu monatomic imprinting recognition layer, and imprinting holes formed by Cu monatomic sites are specifically coordinated with glucose molecules. The master control circuit controls sampling and determines the glucose concentration based on the photocurrent signal. Noninvasive sampling and monatomic imprinting photoelectric detection are fused, sampling-detection integration is achieved, and the device has the advantages of being noninvasive, painless, high in selectivity, high in interference resistance, comfortable to wear and the like and is suitable for continuous monitoring of blood glucose.

The present disclosure relates to systems, devices and methods for receiving biosensor data acquired by a medical device, e.g., relating to glucose concentration values, and controlling the access and distribution of that data. In some embodiments, systems and methods are disclosed for monitoring glucose levels, displaying data relating to glucose values and metabolic health information, and controlling distribution of glucose data between applications executing on a computer, such as a smart phone. In some embodiments, systems and methods are disclosed for controlling access to medical data such as continuously monitored glucose levels, synchronizing health data relating to glucose levels between multiple applications executing on a computer, and / or encrypting data.

This document discusses, among other things, systems and methods to compensate for the effects of temperature on sensors, such as analyte sensor. An example method may include determining a temperature-compensated glucose concentration level by receiving a temperature signal indicative of a temperature parameter of an external component, receiving a glucose signal indicative of an in vivo glucose concentration level, and determining a compensated glucose concentration level based on the glucose signal, the temperature signal, and a delay parameter.

The invention discloses a preparation method and application of a glucose sensor based on an acupuncture needle, the sensor is prepared based on a specific structural design and a molecular recognition mechanism by taking the acupuncture needle as a substrate, and high-specificity and high-sensitivity detection of glucose concentration is realized through drain current change. According to the present invention, the biosensor has the ultra-wide dynamic detection range (0-40 mmol.L <-1 >), covers the clinical range from hypoglycemia to severe hyperglycemia, has the low detection limit of 54 mol.L <-1 >, has excellent grid regulation and control ability, shows the significant on-off ratio and the rapid response characteristic during the detection process, and meets the requirements of the biosensor on sensitivity and real-time performance; and the device has quick response capability to grid voltage change, so that the device has good stability and repeatability under repeated operation, and a direction and a basis can be provided for the development of a blood glucose monitoring technology with continuous, real-time and accurate characteristics.

The invention provides schizochytrium limacinum FJ-1 and application thereof in production of phospholipid type EPA (eicosapentaenoic acid). The application comprises the following steps: taking a cryopreserved schizochytrium limacinum strain, and inoculating the schizochytrium limacinum strain into a liquid seed culture medium for pre-culture; the method comprises the following steps: inoculating pre-cultured schizochytrium limacinum into a fermentation tank according to the inoculum size of 5-10% v / v, culturing for 48-60 hours at the sea salt concentration of 0.5-1% w / v, fermenting for 48-72 hours, supplementing sea salt to a culture medium in batches until the sea salt concentration is 3-5% w / v, and continuously culturing for 96-120 hours by controlling the glucose concentration and the yeast extract concentration through supplementing materials. The technical problem that the synthesis efficiency of schizochytrium limacinum phospholipid type EPA is low is solved through precise regulation and control of salinity stress, an efficient, economical and generalizable innovative scheme is provided for industrial production of functional lipid, and the method is suitable for large-scale production.

The invention provides an efficient fermentation production method and application of acid type sophorolipid, and the production method comprises the following steps: (1) in a growth stage, adding a composite nitrogen source and growth factors into a basic culture medium to promote the growth of zymophyte and realize high-density fermentation, the zymophyte being candida utilis subjected to genetic engineering modification; (2) in the product synthesis stage, mixed syrup and vegetable oil are used as carbon sources, and the concentration of glucose in a fermentation tank is maintained at 1-5g / L and the concentration of residual oil is maintained at 1-5g / L by regulating and controlling the material supplementing rate. The fermentation production method provided by the invention solves the problems of slow thallus growth, long fermentation period, low production intensity, uncontrollable product composition, more byproducts, peculiar smell of fermentation tail gas and the like in the existing sophorolipid production process, and has industrial application value.

The invention relates to the crossing field of computer technology and biomedical engineering, and discloses a miniaturized intelligent blood glucose monitoring and analyzing method integrating edge computing. The method comprises the steps that glucose concentration and time sequence multi-source physiological signals are collected through a wearable sensor; performing data segmentation and multi-modal feature alignment based on the circadian rhythm; inputting the features into a lightweight student model obtained through knowledge distillation for blood glucose prediction; and the reasoning frequency is adaptively adjusted in combination with a memory feedback mechanism. The system comprises a sensing unit, a preprocessing unit, a lightweight model execution unit and a risk early warning unit. While the prediction precision is guaranteed, the model is compressed to be within 150 thousand parameters, a 512 KB on-chip memory is adapted, and low-power-consumption and high-stability end-side continuous blood glucose monitoring and early warning are achieved.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a device and a method for gradually adjusting the glucose concentration of an in-vitro embryo culture solution. The device comprises at least two miniature liquid storage bins, wherein each miniature liquid storage bin is filled with a high-concentration glucose solution in advance; the slow release channel is respectively connected with each micro liquid storage bin and the culture solution main container; the culture solution main container is used for accommodating an embryo and a basal culture medium; the control unit is used for sequentially opening the release ports of the miniature liquid storage bins, so that the high-concentration glucose solution sequentially flows into the culture solution main container through the slow release channels. The technical means of staged closed-loop micro sugar control is introduced into the structure and the using method of the device, so that the accurate adjustment of the sugar concentration of the in-vitro embryo culture solution is realized, the requirements of embryos in different development stages can be effectively met, and the embryo culture quality and the subsequent development potential are remarkably improved.

A system for monitoring glucose concentration that includes a sample holder configured to contain a sample and a radio frequency transmitter configured to emit microwave radiation through the sample. The radio frequency transmitter comprising a radio frequency generator and a first antenna. The radio frequency receiver is configured to receive microwave radiation transmitted from the radio frequency transmitter and passed through the sample, and convert the microwave radiation passed through the sample to an analog signal. The radio frequency receiver comprising a second antenna. The system also includes an analog readout circuit configured to receive the analog signal and process the analog signal into a modified analog signal, and a digital signal processing circuit configured to receive the modified analog signal, convert the modified analog signal into a digital signal, and determine the glucose concentration of the sample from the digital signal.

The invention discloses a sensor for detecting the concentration of trace glucose in saliva, the sensor is coated with a glucose conversion layer, and the glucose conversion layer is used for converting glucose in to-be-detected saliva into hydrogen ions, so that the hydrogen ions are captured by the sensor and converted into electric signals. The sensor disclosed by the invention is coated with the glucose conversion layer, and can correspond to the blood glucose level of a testee by detecting the glucose content of oral saliva. The sensor is simple in structure, convenient to manufacture, high in glucose detection sensitivity to oral saliva and capable of detecting the content of trace glucose in the oral saliva.

The invention discloses a substrate integrated waveguide microwave sensor for glucose detection, and aims to solve the problem of low sensitivity of an existing non-invasive glucose sensor. The substrate integrated waveguide microwave sensor for glucose detection comprises an input feeder line, an integrated waveguide and a split ring resonator, the integrated waveguide is a fan-shaped microstrip line, the split ring resonator is arranged in the middle of the fan-shaped microstrip line, the input feeder line is located on the central axis of the integrated waveguide, and the split ring resonator is located in the middle of the integrated waveguide. One end of the input feeder line is located on the outer side of the arc of the integrated waveguide, the other end of the input feeder line extends into the integrated waveguide, and a metalized through hole array is arranged in the circumferential direction of the arc of the integrated waveguide. The SIW structure and the SRR structure are combined to be used for glucose detection, efficient space utilization and excellent electromagnetic characteristics are achieved, the performance and sensitivity of the sensor are remarkably improved, and the sensor shows excellent sensitivity and linear response in the glucose concentration range of 0-1000 mg / dL.

The invention relates to the technical field of glucose detection, and discloses a method for detecting glucose in d-ribose fermentation broth, which comprises the following steps: step 1, diluting a d-ribose fermentation broth sample and adjusting the d-ribose fermentation broth sample to a predetermined pH value to form a pretreated sample; 2, removing impurities and concentrating target components through solid-phase extraction to obtain a treated sample; 3, separating the sample by using high performance liquid chromatography, setting a mobile phase, a flow rate and a chromatographic column temperature, and separating to obtain a glucose component; and 4, injecting the separated components into a mass spectrum system on line, and detecting a characteristic ion signal of glucose. The high-sensitivity and accurate detection of glucose in the d-ribose fermentation liquor can be realized by adopting a high-performance liquid chromatography and mass spectrometry combined technology. Through optimized sample pretreatment and mobile phase selection, interference of other carbohydrates on glucose detection is reduced, and accurate determination of glucose concentration is ensured.

The invention relates to the technical field of spectral analysis, and discloses a glucose solution concentration detection method and system based on a near infrared spectrum. The method comprises the following steps: acquiring transmission light intensity of a sample and a reference liquid by using a narrow-band near-infrared LED light source, calculating absorbance characteristics, screening a dynamic wavelength set based on sensitivity, and constructing a concentration prediction model to realize accurate detection of concentration. Compared with the prior art which depends on single wavelength or lossy detection, especially under the condition of temperature and humidity fluctuation or unstable light source power, the technical problem that high-precision concentration measurement cannot be realized is solved. High-precision glucose concentration detection under complex environmental conditions is realized, so that the problem of error accumulation caused by environmental interference is avoided, the detection precision, stability and environmental adaptability of the system are improved, and the reliability and accuracy of glucose solution concentration detection are improved.

The invention discloses saccharomyces cerevisiae recombinant bacteria for efficiently producing terpenoids as well as a construction method and application of the saccharomyces cerevisiae recombinant bacteria. According to the construction method, saccharomyces cerevisiae rich in terpenoid precursor farnesyl pyrophosphate is taken as a starting strain, the GAL80 protein level in a galactose induction system is regulated and controlled by introducing an N-Degron strategy, and meanwhile, a glucose concentration response type promoter HXT1 is introduced to effectively regulate and control the expression of GAL80 from the transcriptional level; the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids can be obtained. The strategy is applied to a saccharomyces cerevisiae engineering strain of beta-elemene and patchouli alcohol, and a remarkable effect is achieved. And in the high-density fermentation process of the saccharomyces cerevisiae, the yield of the beta-elemene is obviously increased and reaches 4818.6 mg / L. The invention provides a solid research basis for sustainable biosynthesis of terpenoids, and has a wide application prospect.

The invention provides a DNA enzyme triple-helix molecular switch detection system adaptive to a personal glucometer as well as a preparation method and application of the DNA enzyme triple-helix molecular switch detection system, and belongs to the technical field of biosensors. The DNA enzyme triple-helix molecular switch disclosed by the invention comprises an aptamer containing two nucleotide arm segments and DNA enzyme covalently coupled with a magnetic bead, two nucleotide arm segments of the aptamer and DNA enzyme form a triple helix structure; the two nucleotide arm segments comprise a nucleotide sequence SEQ ID NO.1 at the 5'end of the aptamer and a nucleotide sequence SEQ ID NO.2 at the 3 'end of the aptamer. According to the invention, the flexibility of an original aptamer is limited by using a terminal fixation strategy, the spatial conformation of the original aptamer is stabilized, the affinity of the aptamer to a target is improved, and the sensitivity of the aptamer to the target is improved by using a triple-helix molecular switch containing DNA enzyme and nano-enzyme-gold nanoparticles. Accurate and sensitive detection of trace pollutants in food is realized by measuring the reduction amplitude of the glucose concentration in the detection liquid through a household glucometer.

A method may comprise (a) inserting an insulin delivery device subcutaneously into a body of a subject, which comprises an amperometric glucose sensor comprising an electrode layer comprising an indicating electrode and underlying a redox-catalytic layer comprising a redox mediator; (b) using said insulin delivery device to deliver an insulin formulation (e.g., comprising a phenol or cresol excipient) subcutaneously to said subject; and (c) using said amperometric glucose sensor to measure a subcutaneous glucose concentration, which comprises using said redox-catalytic layer to allow electron transfer from subcutaneous glucose to said indicating electrode sufficient to cause a response of said amperometric glucose sensor at an applied bias potential of no more than +250 millivolts, wherein (b) and (c) are performed at the same time for at least one hour, while maintaining a sensor sensitivity of at least 50% of an initial sensor sensitivity for up to one hour.

In an aspect, the present disclosure provides a method comprising: (a) positioning an insulin delivery device on a body of a subject via a single puncture site in said body of said subject; (b) using said insulin delivery device to deliver an insulin or insulin analog formulation to said subject via said single puncture site; and (c) using a sensor of said insulin delivery device to measure a glucose concentration in said body of said subject via said single puncture site, wherein (b) and (c) are performed at substantially the same time for a time period of at least 1 hour subsequent to said positioning in (a), while maintaining a sensitivity of said sensor of at least 50% of an initial sensitivity of said sensor.

The invention provides a calibration method for real-time dynamic blood glucose monitoring. The calibration method comprises the steps that 1, weak current signals generated by a glucose sensitive electrode are obtained regularly; 2, filtering the detected weak current signal by adopting a sliding window double-layer screening algorithm; 3, monitoring the body temperature of the human body, compensating the temperature based on the filtered weak current value, and estimating the glucose concentration after temperature compensation; 4, monitoring the PH value of the human blood, compensating the PH value according to the filtered weak current value, and estimating the glucose concentration after PH value compensation; and 5, combining the glucose concentrations after temperature and pH value compensation, and carrying out filtering processing on the combined glucose concentrations by adopting a parameter compensation filtering algorithm to obtain a final glucose concentration estimation result.

The invention discloses a serum glucose detection method based on MOFs nano-enzyme, and belongs to the technical field of serum glucose detection.The method comprises the following steps that GOD and human serum are mixed and react at 37 DEG C for 10 minutes, and GOD catalyzes glucose in the serum to react to generate H2O2; then, a mixed solution of MIL-53Fe and TMB is added into the system, and the reaction continues to be carried out for 10 minutes at the temperature of 37 DEG C; in the process, MIL-53Fe plays a catalytic role, H2O2 is promoted to be subjected to a reduction reaction to generate. OH, meanwhile, TMB is oxidized into oxTMB, the solution is turned into blue from colorless, and a remarkable absorption peak is formed at the wavelength of 652 nm. The concentration of glucose in serum is in positive correlation with the amount of generated. OH, so that under the catalytic action of MIL-53Fe, the amount of generated oxTMB is increased along with the increase of the concentration of glucose, and the blue color of the solution is deepened; the serum glucose detection method based on the MOFs nano-enzyme can be used for detection in a neutral solution, and has excellent accuracy, precision, sensitivity and reproducibility.

The invention relates to the technical field of data processing, and particularly discloses a wearable device-based blood glucose data acquisition and processing system and method, which utilize an artificial intelligence technology based on deep learning to perform continuous monitoring and data analysis on subcutaneous intertissue fluid glucose concentration data before and after medication of a patient. According to the method, the time sequence change characteristics of the blood glucose concentration before and after the medication of the patient are captured, and the dynamic change information of the blood glucose concentration of the patient under the influence of the medicine is mined in combination with the medicine intake information of the patient, so that the adjustment suggestion of the medicine intake of the patient is intelligently generated. Therefore, the diabetic can be helped to manage the blood glucose level more scientifically and reasonably, complications are reduced, and the life quality is improved.

The invention relates to the technical field of medical detection equipment, and particularly provides a wearable sweat glucose detection system which comprises a sweat collecting and conveying unit which is provided with a sweat inlet and a sweat flow channel which are communicated with each other and is used for collecting and conveying sweat secreted by skin; the electric signal acquisition unit is arranged on the sweat flow channel, comprises a counter electrode, a working electrode and a reference electrode which are sequentially arranged along the flowing direction of sweat in the sweat flow channel, and is used for acquiring a real-time electric signal related to the glucose concentration; the working electrode comprises a porous alloy structure layer composed of platinum and nickel and a glucose oxidase layer located on the surface of the porous alloy structure layer; the signal processing unit is used for acquiring the real-time glucose concentration according to the real-time electric signal and a preset change curve of the electric signal about the glucose concentration; the system can effectively improve the sensitivity of glucose concentration detection and reduce the dosage of precious metal.

The present invention relates to an ATR sensor (10) for determining the concentration of glucose in a fluid, comprising a sensor housing (12) in which at least the following sensor components are accommodated: an infrared radiation source (22); an ATR element (24) which is designed to transmit infrared radiation emitted by the infrared radiation source (22) under total reflection at at least one boundary surface of the ATR element (24); at least one infrared detector (40) which is designed to detect infrared radiation transmitted by the ATR element (24) and to output a corresponding infrared measurement signal;and at least two infrared bandpass filters (36a, 36b) arranged between the ATR element (24) and the infrared detector (40), each of the infrared bandpass filters (36a, 36b) being configured to transmit only infrared radiation having a wavenumber within a predetermined wavenumber window (F1, F2). A first of the infrared bandpass filters (36a) is configured to transmit only infrared radiation having a wavenumber within a first wavenumber window (F1) of 1005 cm at a temperature of 37°C; -1 up to 1025 cm -1 A second infrared bandpass filter (36b) is at a temperature of 37 °C for infrared radiation with a wavenumber in a second wavenumber window (F2) of 1075 cm -1 up to 1095 cm -1 permeable.

According to an aspect, there is provided a computer-implemented method (200) of determining a concentration of glucose in a subject's blood, the method comprising acquiring (202) first measurement data indicative of a response resulting from an electromagnetic (EM) signal interacting with the subject's blood in a body part of the subject, the EM signal having been emitted from a device in contact with the body part of the subject; acquiring (204) second measurement data indicative of a pressure applied to the body part of the subject by the device; and using (206) a predictive model to infer a concentration of glucose in the subject's blood from the first measurement data and the second measurement data, the predictive model having been trained to infer a concentration of glucose in the subject's blood from the first measurement data and the second measurement data.

The invention relates to a system for storing or cultivating organ or tissue models and its use. The system is designed for verifying the functionality of the harvested organ or tissue models and allowing them to be processed. The system can be used for cultivating organ and / or tissue models harvested from donors and / or prepared using other techniques, such as using 3D and / or 4D bioprinting or electrospinning techniques, and comprises a chamber (2) for the organ or tissue models, which is equipped with means for measuring and adjusting the temperature of the perfusion liquid in the chamber, a perfusion liquid container (1), which is equipped with means for measuring and adjusting the temperature of the perfusion liquid therein, means (4) for measuring the glucose concentration in the perfusion liquid and adding glucose to the perfusion liquid in a metered amount, means (3) for measuring the pH of the perfusion liquid and adding a substance for adjusting the pH of the perfusion liquid in a metered amount, means for non-contact mixing of the perfusion liquid, and means for measuring and controlling the flow parameters of the perfusion liquid.

The invention discloses a corneal glucose real-time non-invasive detection system and method, and belongs to the technical field of glucose real-time non-invasive detection.The method comprises the following steps that corneal reflection spectrum, tear film thickness change and environment temperature and humidity data are obtained in real time, and spectrum compensation parameters are generated through a temperature and humidity compensation model; outputting a tear film refractive index correction value in combination with a refractive index correction model, and correcting the reflection spectrum through a combined correction formula; then, calculating a reference reflection spectrum and a deviation value based on historical data, extracting statistical characteristics, and fusing confidence to obtain a confidence factor for adjusting the deviation value; if the deviation exceeds the threshold value, replacing the corrected data with the reference data; and finally, calculating the glucose concentration according to the processed spectrum, and determining the health grade according to a preset comparison table. The high-reliability non-invasive blood glucose health monitoring closed-loop method and system are constructed through multi-source data fusion correction, intelligent deviation optimization and a confidence coefficient decision mechanism.

The invention relates to a method for monitoring host physical sign parameters on line and a multi-working electrode system. The accuracy of glucose monitoring can be improved. The method comprises the following steps: acquiring a first physical sign parameter of a host by adopting a first working electrode; acquiring a second physical sign parameter of the host by adopting a second working electrode; obtaining a target glucose concentration of the host based on the first physical sign parameter and the second physical sign parameter; the first working electrode and the second working electrode are arranged on the same substrate and can independently obtain a first physical sign parameter and a second physical sign parameter at different depths of the host tissue. The two working electrodes can effectively detect the concentrations of analytes at different depths, and by combining the sign parameters generated by the different working electrodes, a more reliable continuous monitoring result can be comprehensively obtained, and the monitoring accuracy is improved.

The invention provides a glucose hydrogel optical fiber sensor and a preparation method thereof. The glucose hydrogel optical fiber sensor comprises an optical fiber and a glucose fluorescence sensing module fixedly carried on the end face of the optical fiber. Wherein the optical fiber comprises a double-layer hydrogel optical fiber which comprises an inner fiber core and an outer cladding. The glucose fluorescence sensing module is formed by fixing a glucose fluorescence probe containing a boric acid group and a terminal amino group through a covalent reaction between the amino group and an epoxy group in the hydrogel network. The glucose hydrogel optical fiber sensor has excellent performance, high sensitivity and good selectivity, and can realize stable and real-time detection of glucose concentration in a complex physiological environment. The sensor is flexible in structure and good in biocompatibility, does not depend on enzyme reaction, and avoids the problem of signal drift caused by enzyme inactivation; meanwhile, the sensor is good in repeatability, can be integrated with other optical sensing modules to realize multi-parameter synchronous monitoring, and is suitable for long-term dynamic monitoring application in vivo.

Provided in the present application are a blood glucose monitoring method and a related apparatus. In the method, the blood glucose level at a first moment can be acquired on the basis of a glucose concentration in interstitial fluid at the first moment, a glucose concentration in the interstitial fluid within a time period before the first moment and a glucose concentration in the interstitial fluid within a time period after the first moment. In the present application, the glucose concentration in the interstitial fluid within the time period after the first moment is taken into consideration, and an additional time period is provided for the diffusion of glucose between the interstitial fluid and blood; therefore, when the blood glucose level drastically changes, the glucose concentration in the interstitial fluid more closely approximates the blood glucose level, and thus the accuracy of blood glucose monitoring can be improved.