23 results about "Dark fermentation" patented technology

The invention belongs to the technical field of biological fermentation hydrogen production. The invention provides a method for producing hydrogen by dark fermentation of pericarp biomass, which comprises the following steps: drying pericarp, crushing, and sieving to obtain powdery pericarp particles; performing acid heat pretreatment on the powdery fruit peel particles to obtain fruit peel pretreatment liquid; and inoculating the clostridium butyricum solution into the pericarp pretreatment liquid, and carrying out dark fermentation under an anaerobic condition to obtain a fermentation gas containing hydrogen. According to the method, the pericarp byproducts are used for dark fermentation for hydrogen production, so that high-value recycling utilization of agricultural wastes is realized; lignin in the pericarp is effectively removed through acid heat pretreatment, the availability of cellulose and hemicellulose is remarkably improved, conditions such as acid concentration, temperature and time are optimized, the inhibition effect of by-products is avoided, and the hydrogen production efficiency is remarkably improved; moreover, the method has the advantages of good stability and high repeatability, and provides a new technical approach for efficient production of clean energy.

The application discloses a three-stage linkage hydrogen production method based on kitchen waste, and relates to the technical field of biological enzymes, and comprises the following steps: step I, pretreatment; step II, dark fermentation: feeding the material treated in step I into a dark fermentation tank, inoculating Clostridium beijerinckii with the preservation number of ATCC NO.35702; step III, photo fermentation: feeding the first fermentation liquor into a photo fermentation tank, inoculating Rhodopseudomonas palustris with the preservation number of CGMCC 1.2180, adding 50 mg / L-100 mg / L MoFe nanoparticles, and collecting the gas produced in the fermentation. The three-stage linkage of the synergistic pretreatment, dark fermentation and photo fermentation realizes the efficient and stable conversion of the kitchen waste into hydrogen.

This invention provides a fluidized bed multi-stage anaerobic process synergistic wastewater treatment and energy recovery system, belonging to the field of wastewater treatment and energy recovery. The system includes a pretreatment module for synergistic wastewater treatment via ultrasonic crushing and enzymatic hydrolysis, a dark fermentation module, an anaerobic digestion module, a microbial fuel cell module, a gas collection module for collecting gases generated by each module, an electrical energy regulation module for applying electrical energy generated by the microbial fuel cell to the dark fermentation and anaerobic digestion modules to form a non-uniform potential field, and a comprehensive regulation module for receiving detection signals and regulating the gas supply. The discharge end of the dark fermentation module is connected to the feed ends of both the anaerobic digestion and microbial fuel cell modules, forming two series paths: dark fermentation-anaerobic digestion-microbial fuel cell and dark fermentation-microbial fuel cell-anaerobic digestion. This invention achieves deep purification of wastewater and synergistic recovery of multiple forms of energy, including hydrogen, methane, and electricity.

The invention discloses a method for improving clostridium anaerobic fermentation hydrogen volume yield and hydrogen production rate and application. According to the method, biochar is added into a dark fermentation culture medium for anaerobic fermentation to obtain hydrogen. According to the method, the clostridium can be adsorbed on the surface and inside the biochar, so that the clostridium is prevented from being directly contacted with a high-concentration glucose-containing culture medium to a certain extent, the inhibition effect of high-concentration glucose is reduced, and the hydrogen production rate and the glucose utilization rate are increased. Moreover, the biochar is alkaline after being dissolved in water, so that the rapid reduction of the pH of the culture system can be relieved. Meanwhile, the porosity of the biochar is also developed, and the biochar can adsorb part of organic acids such as acetic acid and butyric acid generated by glucose metabolism, so that the inhibition effect of the organic acids on hydrogen production is relieved, and the hydrogen production rate and the glucose utilization rate are further improved. In addition, the method also has the characteristics of low cost, simplicity and convenience in operation and the like, and large-scale industrial application is easy to realize.

The application discloses a method for improving hydrogen production performance of Clostridium butyricum by using hematite nanoparticles, and belongs to the technical field of biological hydrogen production and microbial metabolism regulation. The method improves the hydrogen production performance by adding hematite nanoparticles into an anaerobic fermentation hydrogen production system of Clostridium butyricum for anaerobic fermentation. The application first discloses a specific double regulation mechanism of hematite nanoparticles on hydrogen production of Clostridium butyricum. By using hematite nanoparticles as a core regulation material, and through precise concentration gradient addition and stage temperature synergistic regulation, the hydrogen production lag period can be greatly shortened, the initial hydrogen production rate can be improved, byproduct synthesis of butyric acid can be inhibited, and the efficient hydrogen production path of acetic acid can be strengthened. The core technical problems that existing iron-based additives cannot shorten the hydrogen production lag period, the initial hydrogen production efficiency is low, and product feedback inhibition is easily caused are solved. The method is simple to operate, has strong industrialization adaptability, and can be widely applied to industrialization batch and continuous production of dark fermentation biological hydrogen production.

The invention relates to a method of producing an ester of a fatty acid and a C1-C4 alcohol, comprising successive steps of: cultivating cells of a microalga strain on a growth medium, harvesting the cultivated cells and concentrating them to obtain a wet cell pellet, suspending said wet cell pellet in an hydroalcoholic solution comprising said C1-C4 alcohol, incubating the cell suspension thus formed so as to form said ester of a fatty acid and a C1-C4 alcohol by transesterification and / or esterification of lipids produced by the microalga cells catalyzed by endogenous enzymes of said cells, and recovering said ester of a fatty acid and a C1-C4 alcohol from said cell suspension. The growth medium can advantageously be a dark fermentation effluent.

The application belongs to the technical field of biological fermentation, and particularly relates to a dark fermentation mixed flora, a culture method thereof and application of the dark fermentation mixed flora in hydrogen production from waste oil reservoirs. In the application, a mixed flora capable of producing hydrogen by using waste oil reservoirs is disclosed, the mixed flora comprising Thermotoga hypogea, Thermotoga elfii, Thermus sp., Propionibacterium freudenreichii, Butyrivibrio fibrisolvens and Pseudomonas aeruginosa. The mixed flora can not only produce hydrogen by using crude oil as raw material, but also improve the flowability of the oil layer, improve the interface between the oil layer and the water layer, and crack heavy components in the crude oil, so that the oil layer can be fully utilized for fermentation hydrogen production, and the efficiency of hydrogen production by microorganisms using waste oil reservoirs is improved.

The invention discloses a dark fermentation-methanation-light fermentation integrated organic wastewater treatment method and system, and relates to the technical field of sewage treatment.The dark fermentation-methanation-light fermentation integrated organic wastewater treatment method comprises the following steps that S1, organic wastewater is put into a dark fermentation reaction zone, organic matters in the organic wastewater are decomposed into organic acids by a first microbiota in the dark fermentation reaction zone to generate hydrogen; s2, enabling a product in the dark fermentation reaction zone to enter a methanation reaction zone; s3, enabling a product in the methanation reaction zone to enter a light fermentation reaction zone; the second microbiota in the methanation reaction zone can convert part of organic substances from the dark fermentation reaction zone into acetic acid, methane and carbon dioxide, so that the components, concentration and pH of products in the dark fermentation reaction zone are adjusted, and the third microbiota in the light fermentation reaction zone can continuously and stably grow and generate hydrogen; therefore, the organic wastewater treatment efficiency and the yield of methane, photosynthetic bacteria and hydrogen are improved.

The present application relates to a kind of hydrogen production device for high concentration organic wastewater applied to hydrogen production technical field, including wastewater pretreatment unit, hydrogen production unit, gas purification unit, hydrogen storage unit and leakage monitoring unit, wastewater pretreatment unit includes grid filter tank and adjusting tank, hydrogen production unit includes dark fermentation module, light fermentation module and intermediate product transfer module, gas purification unit includes gas-water separator, mixed buffer tank, PTFE membrane separator, lye washing tower, hydrogen storage unit includes hydrogen storage tank, and leakage monitoring unit includes tank monitoring mechanism;The hydrogen production device in the application can efficiently convert high concentration organic wastewater into high-purity hydrogen, realize waste treatment and waste into treasure, and the leakage monitoring unit can conduct real-time monitoring of the hydrogen storage unit in all directions based on the active airflow guiding mechanism.Once hydrogen leakage occurs, it can be discovered and alarmed in time, significantly improving the safety of the hydrogen production process.

The invention relates to a hydrogen production device for high-concentration organic wastewater, which is applied to the technical field of hydrogen production, and comprises a wastewater pretreatment unit, a hydrogen production unit, a gas purification unit, a hydrogen storage unit and a leakage monitoring unit, the hydrogen production unit comprises a dark fermentation module, a light fermentation module and an intermediate product transfer module, the gas purification unit comprises a gas-water separator, a mixing buffer tank, a PTFE membrane separator and an alkali liquor washing tower, the hydrogen storage unit comprises a hydrogen storage tank, and the leakage monitoring unit comprises a storage tank monitoring mechanism; according to the hydrogen production device, high-concentration organic wastewater can be efficiently converted into high-purity hydrogen, waste is treated with waste, waste is turned into wealth, the leakage monitoring unit can comprehensively monitor the hydrogen storage unit in real time on the basis of an active airflow guiding mechanism, once hydrogen leakage occurs, the leakage can be found in time, an alarm is given, and the hydrogen production efficiency is improved. And the safety of the hydrogen production process is obviously improved.

The invention discloses a three-stage linkage hydrogen production method based on kitchen waste, and relates to the technical field of biological enzymes, and the method comprises the following steps: step I, pretreatment; step II, dark fermentation: the materials treated in the step I are fed into a dark fermentation tank, and clostridium beijerinckii with the preservation number of ATCC (American Type Culture Collection) NO.35702 is inoculated into the dark fermentation tank; step III, light fermentation: feeding the first fermentation liquor into a light fermentation tank, inoculating rhodopseudomonas palustris with the preservation number of CGMCC (China General Microbiological Culture Collection Center) 1.2180, adding MoFe nano particles with the concentration of 50 mg / L-100 mg / L, and collecting gas generated by fermentation. Through three-stage linkage of collaborative pretreatment, dark fermentation and light fermentation, efficient and stable conversion from kitchen waste to hydrogen is realized.

The invention discloses a biological hydrogen production method, which relates to the technical field of biological energy, and comprises the following steps: S1, under the condition of no illumination, anaerobic bacteria and an organic reaction solution are subjected to a reaction to prepare hydrogen and dark fermentation tail liquid; s2, the dark fermentation tail liquid enters a photoreactor, photosynthetic bacteria are added, and hydrogen is prepared; wherein the photosynthetic bacteria are loaded on the composite carrier, and the specific loading method comprises the following steps: pretreating vermiculite, then soaking the vermiculite in a bacteria solution containing the photosynthetic bacteria for continuous culture, and taking out the vermiculite after the culture is finished. The photosynthetic bacteria are loaded on the composite carrier through a dark-light combined biological hydrogen production process, so that on one hand, strains can be concentrated to realize immobilization and the treatment efficiency of the strains can be improved, and on the other hand, the light absorption rate of the photosynthetic bacteria can be further improved by adding the light guide particles on the composite carrier, so that the photosynthetic bacteria can be effectively degraded. The strain treatment efficiency is further improved.

The invention discloses a nitrogen limitation-based clostridium pasteurianum-rhodobacter sphaeroides coupling fermentation hydrogen production method and application thereof. The method comprises the following steps: performing dark fermentation on activated clostridium pasteurianum in a nitrogen-free culture medium in an argon environment to produce hydrogen; and adjusting the pH value of the obtained culture solution to 7.0-8.0, selectively sterilizing, inoculating activated rhodobacter sphaeroides, and carrying out light fermentation in an argon environment to produce hydrogen. The clostridium pasteurianum fermentation liquor can be directly subjected to rhodobacter sphaeroides photofermentation hydrogen production culture only by simply adjusting the pH value without operations such as centrifugal removal of thalli. According to the method, excessive proliferation of thalli is inhibited through nitrogen limitation, the flow direction of a carbon source is saved, high expression of nitrogenase is induced, and a second efficient hydrogen production way except hydrogenase is opened up, so that the hydrogen yield is further increased while the cost of a nitrogen source is reduced. The hydrogen production yield reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The application discloses a method for producing hydrogen through Clostridium pasteurianum-Rhodobacter sphaeroides coupling fermentation based on nitrogen limitation and application thereof. In the method, activated Clostridium pasteurianum is subjected to dark fermentation for producing hydrogen in a nitrogen-free culture medium and in an argon environment; then, the pH value of the obtained culture solution is adjusted to 7.0-8.0, the activated Rhodobacter sphaeroides is inoculated after selective sterilization, and the light fermentation for producing hydrogen is carried out in the argon environment. In the application, the fermentation solution of Clostridium pasteurianum does not need to be subjected to centrifugal removal of bacterial bodies and the like, and only needs to be simply adjusted in pH value, so that the light fermentation for producing hydrogen of Rhodobacter sphaeroides can be directly carried out. In the application, the nitrogen limitation is used to inhibit excessive proliferation of bacterial bodies, save carbon source flow, induce high expression of nitrogen fixation enzyme, and open a second high-efficiency hydrogen production path in addition to hydrogenase, so that the hydrogen production yield is further improved while the cost of nitrogen source is reduced. The hydrogen production yield of the application reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The invention discloses a bio-based metal carbon material, a preparation method thereof and application of the bio-based metal carbon material in dark fermentation biological hydrogen production, and relates to the technical field of modified biochar composite materials and the technical field of hydrogen production. The preparation method comprises the following steps: preparing a biochar material by taking corn straw powder as a raw material; mixing the biochar material with one of soluble ferric salt, soluble cobalt salt and soluble nickel salt; adjusting pH, adding a proper amount of ammonia water, stirring and reacting, and standing to obtain a precipitate; and in an inert gas atmosphere, heating the precipitate to a pyrolysis temperature of 700-900 DEG C in a programmed manner at a heating rate of 5-15 DEG C / min, and maintaining the pyrolysis temperature for 1.5-3 hours to obtain the bio-based metal carbon material. The bio-based metal carbon material developed by the invention is modified by adopting a single metal element, the metal addition amount is low, and the cost can be effectively controlled. The material can promote microbial metabolism to incline towards an acetic acid path, and can significantly promote the hydrogen production process. The material has a remarkable magnetic property, and the recycling convenience in application is greatly improved.

The invention relates to a method of producing an ester of a fatty acid and a C1-C4 alcohol, comprising successive steps of: cultivating cells of a microalga strain on a growth medium, harvesting the cultivated cells and concentrating them to obtain a wet cell pellet, suspending said wet cell pellet in an hydroalcoholic solution comprising said C1-C4 alcohol, incubating the cell 5 suspension thus formed so as to form said ester of a fatty acid and a C1-C4 alcohol by transesterification and / or esterification of lipids produced by the microalga cells catalyzed by endogenous enzymes of said cells, and recovering said ester of a fatty acid and a C1-C4 alcohol from said cell suspension. The growth medium can advantageously be a dark fermentation effluent.

The invention relates to biochar, a preparation method thereof and application of the biochar in coproduction of hydrogen and bio-fertilizer through anaerobic fermentation of bone meal, and the application comprises the following steps: uniformly mixing the bone meal, the biochar, an anaerobic fermentation inoculum and distilled water to construct anaerobic dark fermentation liquor, adjusting the initial pH value of the anaerobic dark fermentation liquor, fermenting under anaerobic and constant-temperature conditions to obtain the biochar. Gas generated in the fermentation process is collected, biogas with hydrogen as the main component is obtained, after fermentation is finished, solid-liquid separation is conducted on fermentation residues, and supernate is the liquid biological fertilizer. According to the method, a proper amount of biochar is added into an anaerobic dark fermentation system taking bone meal as a main fermentation substrate, so that the problems of product inhibition and acidification in the bone meal fermentation process can be effectively relieved, the yield and the yield of hydrogen are remarkably improved, degradation and mineralization of the substrate are promoted by adding the biochar, and the biochar has a good application prospect. More nitrogen and phosphorus elements are released and dissolved in the fermentation liquor, so that the value of the fermentation liquor as a liquid biological fertilizer is remarkably improved.

This application relates to the field of bioenergy technology, specifically to a wastewater treatment device for dark-photocatalytic fermentation biohydrogen production. This device combines dark fermentation biohydrogen production with anaerobic ammonia oxidation to remove some organic matter and nitrogen oxides from wastewater, solving the problem of high concentrations of ammonia nitrogen in wastewater inhibiting hydrogen production efficiency. Photocatalytic fermentation further utilizes the dark fermentation products to produce hydrogen and converts sulfides into sulfates, thereby degrading the impact of organic acids and sulfides in the wastewater on subsequent water treatment technologies, reducing biotoxicity, and improving energy conversion efficiency. A nitrification reactor is combined with dark fermentation hydrogen production, and anaerobic ammonia oxidation is carried out in a cyclical manner to improve nitrogen removal efficiency, realizing an integrated device for organic wastewater hydrogen production and wastewater treatment.

This invention provides a method for hydrogen production from organic wastewater through dark fermentation, belonging to the field of fermentation hydrogen production technology. The method includes: mixing organic wastewater, biochar loaded with metal nanoparticles, and a domesticated butyric acid-producing mixed bacterial community; performing micro-electrolysis and bioactivation treatment to obtain a pretreated solution; then subjecting the pretreated solution to hydrogen-producing phase fermentation treatment, adding an electron mediator to obtain a fermentation broth containing volatile acids; further, subjecting the fermentation broth to de-inhibition phase treatment, using adsorbent materials to adsorb volatile acids, and simultaneously converting some of the adsorbed volatile acids into butyric acid through acid-producing bacteria to obtain a post-fermentation solution; electrolyzing the post-fermentation solution, utilizing electroactive hydrogen-producing bacteria for extracellular electron transfer to convert residual organic matter in the solution into hydrogen gas to obtain crude hydrogen, which is then purified to obtain hydrogen gas. This invention solves the problems of low utilization rate of organic wastewater, inhibition of hydrogen production by volatile acids, and the impact of environmental parameter fluctuations on yield in existing processes.

The invention discloses a method for improving the fermentation level of microalgae astaxanthin through plant hormone pretreatment, and belongs to the technical field of microalgae biology. According to the method, firstly, under the dark condition, two plant hormones including brassinolide and cytokinin are utilized to conduct pretreatment on a Chromococcus zoffii seed solution so as to increase the number of plastids, and then through shake-flask culture and fermentation tank fed-batch fermentation, it is proved that the strategy can promote accumulation of astaxanthin of Chromococcus zoffii under the nitrogen deficiency condition. The appropriate concentrations of the two plant hormones are both low, high light stress is not needed, the step operation is simple and convenient, and the production cost is reduced.

The invention provides a method for preparing methane by anaerobic fermentation of straws, and relates to the technical field of production methods of biogas. The method for preparing methane by anaerobic fermentation of straw comprises the following steps: mixing kitchen waste with anaerobic granular sludge, adjusting the pH value to 5.5-6.2, and then carrying out dark fermentation for hydrogen production to obtain dark fermentation biogas slurry; the dark fermentation biogas slurry in the step S1 is used for pretreating straw, and then anaerobic fermentation is carried out to produce methane. The dark fermentation biogas slurry prepared by a specific process is adopted to pretreat the straw, then anaerobic fermentation is performed to produce methane, and the gas production effect is good. The dark fermentation biogas slurry pretreated straw provided by the invention is subjected to anaerobic fermentation to produce methane, multi-stage utilization of organic wastes and simultaneous production of two green energy sources, namely hydrogen and methane are also realized, the dark fermentation biogas slurry is used for anaerobic fermentation to produce methane, and compared with single anaerobic fermentation biogas slurry, the resource utilization rate of the organic wastes is increased, and the production cost is reduced. And a theoretical basis is provided for follow-up hydrogen-alkane co-production practice.

The invention discloses a method for producing hydrogen by converting tobacco waste fermentation hydrogen production wastewater through a microbial electrolysis cell, which comprises the following steps: (1) pretreating tobacco waste, naturally airing and crushing broken tobacco leaves and the like, and sieving; (2) preparing a clostridium perfringens seed culture medium and culturing a seed solution; (3) inoculating the seed solution into a dark fermentation culture medium according to an inoculum size of 10-20%, and fermenting at 35 + / -1 DEG C and 120 r / min to obtain wastewater containing acetic acid and the like, the dark fermentation culture medium containing the pretreated tobacco waste and the like and having a pH value of 6.47; (4) preparing a microbial electrolysis cell inoculation bacterium source, wherein the microbial electrolysis cell inoculation bacterium source is prepared by performing dilution, microwave pretreatment and the like on cow dung or sheep manure compost; (5) enriching the anode by taking the diluted fermentation wastewater as a substrate in the single-chamber air cathode microbial fuel cell; and (6) transferring the anode to a microbial electrolysis cell reactor, blowing by using the diluted wastewater as a substrate, and producing hydrogen at 36 + / -1 DEG C and 0.4-0.9 V. The method can utilize the tobacco waste and the fermentation wastewater to relieve pollution, realize stepped hydrogen production and improve the hydrogen yield.

This invention discloses a hydrogen production process using enzymatic hydrolysis and dark fermentation coupled with high-pectin biomass, belonging to the field of bio-fermentation hydrogen production technology. First, a magnetic biochar-based solid acid catalyst is prepared using camellia oleifera shells as raw material. This catalyst is mixed with navel orange pomace and pretreated hydrolyzed at 110-130℃ to directionally hydrolyze pectin and adsorb inhibitors. Then, the catalyst is recovered by magnetic separation. Next, a stepwise enzymatic hydrolysis is employed: first, pectinase is added to remove the pectin barrier, then cellulase is added to hydrolyze cellulose, obtaining an enzymatic hydrolysate rich in glucose and galacturonic acid. Finally, hydrogen-producing mixed bacteria acclimated to a pectin-cellulose composite carbon source are inoculated for dark fermentation. This invention significantly reduces material viscosity, improves enzymatic hydrolysis efficiency and substrate conversion rate through the synergistic effect of each step. Furthermore, the catalyst can be magnetically recovered and recycled, resulting in low cost and environmental friendliness, providing a new approach for the resource utilization of high-pectin agricultural waste.