52 results about "Dark fermentation" patented technology

The invention discloses a soil remediation agent for heavy metal fixing. Plant ash, livestock manure, corn straw, rapeseed residues, Jerusalem artichoke extract, glycine, chitosan and a fermented microbial agent are used as main components, and sodium hexametaphosphate, montmorillonite, bentonite, weathered coal, charcoal, polylactic acid fiber, manganese nitrate, copper sulfate, magnesium chloride, calcium hydroxide, a stabilizer and an antioxidant are added to prepare the soil remediation agent for heavy metal fixing by being assisted by spray drying, pressurized calcination, high-temperature mixing, ultrasonic smashing, quick mixing, dark fermentation and other processes. The soil remediation agent for heavy metal fixing is reliable in soil restoration performance, can effectively reduce the heavy metal content in soil and is pollution-free to the environment.

The invention relates to a method for treatment of starch wastewater and synchronous energy production by coculture of bacteria and microalgaes and relates to the method for treatment of the starch wastewater and synchronous energy production by coculture of the bacteria and the microalgaes. The invention aims at solving the problem that the hydrogen production is reduced because a large amount of volatile organic acids are produced in the existing dark fermentation hydrogen production process and the method comprises the following steps: 1) taking the starch wastewater, regulating the pH and putting the wastewater into a closed reactor; 2) performing pretreatment on a hydrogen production inoculation matter to obtain a mixture containing hydrogen production bacteria; and 3) mixing the mixture containing the hydrogen production bacteria with the microalgaes, inoculating into the starch wastewater, further putting into a shaking table and performing shaking culture to complete the process. According to the method provided by the invention, the wastewater is used for fermentation and energy production, so that the wastewater is effectively treated, the environmental pollution is reduced, and a method for synchronous preparation of clean energy is provided. According to the method provided by the invention, the energy production is improved by more than 95%, and the sewage treatment efficiency is improved by more than 170%. The method provided by the invention is applied to the field of bio-energy and sewage treatment.

The invention relates to the technology of biomass energy utilization, and aims to provide a kitchen waste and sludge hydrogen-producing acidification pretreatment method capable of increasing the methane production rate. The method comprises the following steps: mixing the kitchen waste and the sludge after crushing pretreatment, using the mixture and a sulfuric acid solution to prepare a mixed liquid, performing hydrolysis on the mixed liquid at 135 DEG C to obtain a starting crude; adding yeast powder into the starting crude, inoculating dark-fermentation hydrogenogens, feeding high-purity nitrogen to build an anaerobic fermentation environment, then performing dark-fermentation hydrogen-producing deep acidification pretreatment at the constant temperature of 37 DEG C, and obtaining a hydrogen-producing deep acidification pretreatment liquid; adding methanogens into the liquid, keeping the anaerobic environment at 37 DEG C, and performing methane fermentation and coproduction. According to the method disclosed by the invention, the hydrogen-producing deep acidification pretreatment is utilized to obviously increase the methane production rate; the rate peak time of the produced methane is reduced by about 50%; the waste treatment and degradation time of the fermentation tank with a unit volume is reduced by about 50%; the waste treatment capacity of the single equipment in unit time is improved by about one time.

The invention relates to nano-iron-loaded biochar, a preparation method of the nano-iron-loaded biochar, and an application of the nano-iron-loaded biochar in a dark fermentation hydrogen production process. Ferrite and soluble starch are taken as raw materials which are subjected to reaction under proper conditions and are dried and charred to obtain the nano-iron-loaded biochar, wherein the maincomponent of the nano-iron-loaded biochar is Fe2O3 / C, and the particle diameter range of the nano-iron-loaded biochar is 2-150nm. The biochar is of an amorphous structure, and Fe2O3 is of a mesoporous structure. By adding the iron-loaded biochar to an anaerobic fermentation system, a synergistic strengthening effect of iron and biochar in the dark fermentation hydrogen production process is realized, so that the hydrogen yield and the process stability are improved. Fe2O3 can not only release iron ions to enhance the activity of fermentative hydrogen producing bacterium hydrogenase but also increase the transfer rate of electrons; and furthermore, the biochar in nano-particles can enrich microorganisms, improve the concentration of hydrogen producing bacteria, buffer acid accumulation andrelieve ammonia inhibition.

The invention provides a method for producing hydrogen by fermentation, in particular to a waste biomass clean and turning into hydrogen energy technology. The invention comprises the following steps: firstly taking the carbon-contained organic waste as the fermentation substrate, the pretreated mixture bacteria of natural livestock manure is dark fermented to get the fermentation liquid of hydrogen and organic acid. Then the photosynthetic bacteria are applied to anaerobicly ferment the fermentation liquid gotten from the above step to produce hydrogen. The invention has advantages of quick hydrogen production, high substrate conversion efficiency, low hydrogen producing cost and standard fermentation liquid emission.

The invention discloses a method utilizing hydrogen-producing acetogens and electricigens to enhance the biological hydrogen production efficiency. The method comprises the following steps: utilizing hydrogen-producing acetogens and electricigens to deeply degrade the organisms, modulating the operation parameters of the dark fermentation technology so as to high efficiently accumulate acetic acid in the end products of the water-generating hydrogen-producing acetogens, taking the generated water as the substrate of an electric catalysis reactor, modulating the additional voltage value, taking the Ni nano particles as the catalyst, the stainless steel as the negative pole, and carbon cloth as the positive pole, and utilizing the extracellular electron transfer effect of the electricigens to further degrade the end products of the hydrogen-producing acetogens to convert the end products into hydrogen gas; thus the phenomenon of fermentation obstacle is weakened, high efficient hydrogen production by utilizing hydrogen-producing acetogens and electricigens is achieved, hydrogen production and pollution treatment are integrated, and the method has the characteristics of cost saving, little energy consumption, and high hydrogen production efficiency.

The invention relates to a method for light-blind-fermentation-coupling-producing hydrogen by culturing sea photosynthetic bacterium with sludge and effluent of sea cultivation section, The method comprises the following steps: proceeding with culturing without light in order to produce hydrogen with sludge and effluent of sea cultivation as the culture medium; culturing with light; proceeding with coupling-production hydrogen by adding light fermenting bacteria in the culture medium in order to improve the releasing quantity of hydrogen and recover the production-hydrogen activity of the sea blind fermentation production-hydrogen bacterial. The method not only reduces the pollution of the environment, but also can release the present nervous energy crisis pressure.

The invention discloses a method for producing hydrogen through kitchen waste enzymolysis and reinforced dark fermentation. The method comprises steps that: impurities are removed from dehydrated sludge discharged from a sewage factory; the sludge is settled for 10 to 20 days under anoxic stress, and is sealed and processed through heat treatment; glucose and peptone are added to the sludge, and the sludge is cultivated and acclimatized under a temperature of 50 to 53 DEG C, such that inoculated sludge is obtained; alpha-amylase and glucoamylase are added to kitchen waste for carrying out saccharification; the saccharified kitchen waste and the inoculated sludge are added into a fermentation bottle, and anaerobic fermentation is carried out under a temperature of 50 to 53 DEG C, such thathydrogen is produced; when the gas production is almost stopped, the reaction is stopped. According to the invention, alpha-amylase and glucoamylase are used for carrying out pre-treatment upon kitchen waste for a short time, such that a fermentation substrate micromolecular glycan which is rich and easy to utilize is provided for hydrogenogens. Therefore, fermentation time is shortened, and hydrogen yield is improved. The method provided by the invention has advantages of simple technology, fast starting, and low cost.

The invention belongs to the field of microbial technology application, and particularly relates to a method for improving the hydrogen yield of hydrogenogens. The hydrogenogens strains are inoculatedinto a culture medium containing 2.5 to 20 mmol / L ferriferous oxides; through dark fermentation culture, the great-quantity hydrogen generation of the hydrogenogens strains can be realized. Through the addition of ferrihydrite and magnetite, the growth of hydrogen production clostridium C.pasteurianum is promoted; the C.pasteurianum substrate conversion efficiency is accelerated; the yield of metabolite products of hydrogen gas, acetic acid and butyric acid is improved. The method provided by the invention has the advantages that the hydrogen yield of the hydrogen production clostridium is greatly improved; the theoretical support is provided for the large-scale production of microbial dark fermentation hydrogen production.

The invention provides a method used for producing hydrogen by taking cellulose as a raw material. The method comprises following steps: 1, microwave acid digestion; 2, enzyme hydrolysis; 3, dark fermentation hydrogen production; 4, photo fermentation hydrogen production; and 5, purification. The method is high in hydrogen yield, hydrogen production efficiency, substrate utilization ratio, and energy conversion efficiency.

The invention discloses a dark-photo fermentation integrated biological hydrogen production device, relates to a dark-photo fermentation integrated biological hydrogen production device and aims at the problem that the efficiency of hydrogen generation through coupling of the dark fermentation bacteria and the photo fermentation bacteria is low in the prior art. The biological hydrogen production device comprises a dark fermentation reaction region, a filter membrane, a photo fermentation region, an illumination system, a dark fermentation gas flow meter and a photo fermentation gas flow meter, wherein a biological hydrogen production reaction container is divided into the dark fermentation reaction region and the photo fermentation reaction region through the filter membrane; a dark fermentation gas flow meter is arranged on the dark fermentation reaction region; the photo fermentation gas flow meter is arranged on the photo fermentation reaction region; the illumination system is arranged at one side of the photo fermentation reaction region; and the working volume of the dark fermentation reaction region is smaller than that of the photo fermentation reaction region. The dark-photo fermentation integrated biological hydrogen production device disclosed by the invention has hydrogen yield of 3048.45ml H2 / L working volume and hydrogen generation rate of 4.08 mol H2 / mol glucose. The dark-photo fermentation integrated biological hydrogen production device is mainly applied to the biological hydrogen production field.

The invention discloses a dark fermentation-light fermentation coupled biological hydrogen preparing process. According to the dark fermentation-light fermentation coupled biological hydrogen preparing process, a bipolar membrane electrodialysis unit is added behind a dark fermentation reactor, the components of the outgoing water obtained after dark fermentation are separated, thus the concentrated acetic acid is obtained, after pH regulation, the concentrated acetic acid is taken as incoming water of a light fermentation reactor, and meanwhile, the not utilized substrate is recycled and returns to the dark fermentation reactor to be reutilized. The product inhibition at the dark fermentation hydrogen production stage can be relieved, the pH of a fermentation system is controlled to be within the optimal range, the substrate is effectively recycled and reutilized, the total utilization ratio of the substrate is improved, the optimal substrate, namely, acetic acid is provided for the light fermentation hydrogen production stage, NH<4+> in the outgoing water obtained by dark fermentation is removed, then the inhibiting for the dinitrogenase of light fermentation bacteria is relieved, meanwhile, the interception and separation for the dark fermentation bacteria are completed, and the steps including centrifugalization, filtering and sterilization of the original process are replaced; the optimal hydrogen production conditions are provided for the dark fermentation and light fermentation stages, and the two-step coupled hydrogen production efficiency adopting the dark fermentation bacteria and the light fermentation bacteria is improved.

The invention provides hydroxyapatite and a preparation method thereof, and new application of the hydroxyapatite in dark fermentation hydrogen production. Hydroxyapatite is added into a dark fermentation hydrogen production system, so that the H2 yield is increased. By adopting a calcining means, the problems of large particle size, non-uniformity and agglomeration in the traditional preparation method can be effectively avoided; the obtained hydroxyapatite particles have high biological activity and good application prospects. The hydroxyapatite is applied to the dark fermentation hydrogen production process, so that the defects of low microorganism quantity, low enrichment capacity, insufficient trace elements, poor enzyme activity and the like in the fermentation process can be overcome; the hydrogen yield of a dark fermentation system is improved by enriching anaerobic fermentation bacteria, promoting the formation of a biological membrane, providing necessary elements and excellent places for the growth and reproduction of microorganisms and the like.

The invention relates to a dark-fermentation biomass energy power introduced power supply system of the renewable energy power grid. The input end of a first electric energy storage device is connected with the output end of a renewable energy power generator, and the output end of the first electric energy storage device is connected with the input end of an AC converter; the electric energy output end of a dark-fermentation biomass energy power generator is connected with the input end of the first electric energy storage device; a controller comprises a processing unit and a control unit, and the processing unit is electrically connected with the first electric energy storage device and the control unit, and the control unit is arranged at the electric energy output end of the dark-fermentation biomass energy power generator. When the generating capacities of multiple renewable energy power generators, influenced by weather factors as wind power and illumination, in the renewable energy power supply system are insufficient, the controller can introduce electric energy converted by the dark-fermentation biomass energy power generator into the power grid of the renewable energy power supply system, so that the load rate of the power grid and the dumping rate of electric power are reduced, stable power supply of the power grid is achieved, and the utilization rate of renewable energy is improved.

The invention provides nickel-doped magnetic carbon and a preparation method and application thereof. The nickel-doped magnetic carbon comprises the following main components: activated carbon, NiFe2O4 and a small amount of Ni(OH)2, wherein the NiFe2O4 and the Ni(OH)2 are uniformly attached to the surface and pores of the activated carbon. The nickel-doped magnetic carbon disclosed by the invention can selectively improve the activity of microorganisms under a medium-temperature condition and optimize a microbial community structure in a hydrogen fermentation system; in addition, under a high-temperature condition, a fermentation path can be converted into butyric acid type fermentation from ethanol type fermentation, and the butyric acid type fermentation is more beneficial for generationof hydrogen.

The invention relates to manganese-doped magnetic carbon, a preparation method of manganese-doped magnetic carbon and application of manganese-doped magnetic carbon in dark fermentation hydrogen production. According to the preparation material, iron salt and manganese salt are used as raw materials, a reaction is conducted under the alkaline and heating conditions, substances generated through the reaction are evenly loaded to the surface and pores of the carbon material, and after drying, manganese-doped magnetic carbon is obtained. Prepared manganese-doped magnetic carbon contains substrates such as MnFe2O4, Fe2O3 and MnCO3 and has the magnetism. Manganese-doped magnetic carbon is applied to the dark fermentation hydrogen production process and can release iron and manganese microelements in a liquid phase and promote activity of microorganisms; the carbon material is used as a carrier capable of gathering microorganisms and forming a biological membrane and provides a good environment for growth and propagation of microorganisms; in addition, manganese-doped magnetic carbon has unique physicochemical properties and can be effectively separated from sludge through the magnetismof manganese-doped magnetic carbon, the carbon material is recycled, and fermentation hydrogen production capacity is improved.

The invention provides a method for two-step fermentation of a starchy raw material with a mixed strain to produce hydrogen. The method comprises the steps of: (1) liquefaction; (2) saccharification; (3) dark fermentation to produce hydrogen; (4) photo-fermentative hydrogen production; and (5) purification. The method provided by the invention has excellent hydrogen production rate, hydrogen production velocity, substrate utilization rate and energy conversion efficiency.

The patent relates to the fields of composite synthesis and clean energy production, and relates to a method for improving hydrogen production performance of dark fermentation by using manganese-dopedmagnetic carbon. The method includes: adding manganese-doped magnetic carbon into an anaerobic fermentation reactor to increasing hydrogen yield and hydrogen production speed. A fermentation substrate in the anaerobic fermentation reactor is composed of carbon source, nitrogen source, inoculums and the manganese-doped magnetic carbon. In the application, the manganese-doped magnetic carbon is applied to the fermentation hydrogen production process, so that defects, such as low quantity of microorganisms and poor enriching capability, insufficient microelements and low enzyme activity, existing in the fermentation can be overcome. By enriching anaerobic fermentation bacteria, generation of bio-film is promoted and required elements and site for growth and reproduction of the microorganismsare supplied, thus finally increasing the productivity of hydrogen.

The invention relates to a dark-fermentation photosynthetic united hydrogen production device and a dark-fermentation photosynthetic united hydrogen production method. The device comprises a support, wherein a photosynthetic biological hydrogen production reactor is placed on the support; a dark-fermentation biological hydrogen production reactor is arranged inside the photosynthetic biological hydrogen production reactor, and the top of the dark-fermentation biological hydrogen production reactor is higher than that of the photosynthetic biological hydrogen production reactor; a light insulation heat preservation layer is arranged between the photosynthetic biological hydrogen production reactor and the dark-fermentation biological hydrogen production reactor. According to the device, the lighted area of the photosynthetic hydrogen production reactor is increased, and the space is saved; a dark-fermentation biological hydrogen production reaction and a photosynthetic biological hydrogen production reaction can be performed at the same time, and the reaction time is shortened; by virtue of the light insulation heat preservation layer, the heat loss of the dark-fermentation reaction is reduced, and the energy consumption is reduced, and the hydrogen production efficiency is improved. In addition, the occupation area of the device is small; the hydrogen production method is easy to operate; modernization is easily realized, and the device can be widely applied to the environmental protection industry, the energy industry, the food industry, the feed industry and other industries, and has a great application prospect.

A method for producing H2, VFAs and alcohols from organic material is disclosed, including the steps of introducing organic material and microorganisms into a completely mixed bioreactor for producing H2, CO2, VFAs, and alcohols; sequestering CO2 in the headspace of the reactor; recovering H2 from the headspace; and recovering a first liquid effluent including microorganisms, VFAs, and alcohols. Also disclosed is a system for producing H2, VFAs and alcohols from organic material, including a completely mixed bioreactor for dark fermentation; an input for supplying microorganisms and the organic material to be broken down; a CO2 trap in the headspace and including a solid hydroxide for sequestration of the CO2 gas from the headspace; and a gas output for removal of a gas effluent including H2 gas from the headspace. The system and method provide higher H2 production rates and a H2 stream is substantially free of CO2.

A waste management and resource recovery system that uses the different waste streams from typical waste sources as fuel or feedstock for its subsystems that, in turn, produce fuel, feedstock or energy for other subsystems such that all the different waste streams are effectively managed. The subsystems include a gas burner for solid and fuel wastes that supplies heat to a hydrothermal processor for saccharification of paper and cardboard. The resulting saccharification broth, along with kitchen wastes and blackwater, are supplied to a bioreactor using dark fermentation to produce hydrogen and volatile fatty acids. The hydrogen and volatile fatty acids are supplied to hydrogen and microbial fuel cells to produce electrical energy for operating the system and potable water. A steam accumulator is added to provide sufficient temperatures and pressures to reach the necessary thermodynamic states for the saccharification process. An enzymatic saccharification processor may also improve the saccharification process.

The invention provides a method for improving dark fermentation hydrogen production performance by using a ferroferric oxide / reduced graphene oxide nano composite material. The addition amount of the ferroferric oxide / reduced graphene oxide nano composite material in the anaerobic dark fermentation system is 100-400mg / L, the ferroferric oxide / reduced graphene oxide nano composite material is prepared from the following components in percentage by mass: 22.79%-27.57% of nano ferroferric oxide and 22.79%-27.57% of reduced graphene oxide. The size of the ferroferric oxide / reduced graphene oxide nano composite material is 80 to 120 nm. The nano ferroferric oxide is uniformly distributed on the surface of the gauze-shaped reduced graphene oxide. According to the structure, the agglomeration phenomenon of ferroferric oxide and reduced graphene oxide is well avoided, the materials can be better dispersed in a dark fermentation hydrogen production system, the interrelation among microorganisms is increased, more electronic channels are constructed, and the hydrogen production performance of hydrogen production microorganisms is improved.

The invention provides a method used for preparing hydrogen via fermentation of starchy raw materials by embedded bacteria. The method comprises following steps: 1, preparation of the embedded bacteria; 2, heating gelatinization of the starchy raw materials, 3, dark fermentation and hydrogen production, 4, photo fermentation and hydrogen production; and 5, purification. The method is high in hydrogen yield, hydrogen production rate, substrate utilization ratio, and energy conversion efficiency.

The invention discloses a preparation method of phellinus linteus sweet wine, and the method is realized by adopting a microorganism mixed fermentation theory. In the traditional sweet wine fermentation preparation process, the method comprises the following steps: inoculating activated phellinus linteus hyphae into sweet wine fermented grains according to a certain amount of (1:100) to (1:250), stirring uniformly and bottling, standing under the temperature of 28 DEG C for dark fermentation for 7 to 14 days to obtain a type of health care sweet wine rich in phellinus linteus polysaccharide active substances. Moreover, an initial product and the culture conditions of sweet wine fermentation are utilized, amplified culture on phellinus linteus mycelium is performed during a process of the sweet wine fermentation, and the nutritional value of the phellinus linteus active substance health care sweet wine is improved. The method is simple, and the prepared phellinus linteus sweet wine has the advantages of high nutritional value, high quality, high curative effect and high value, is suitable for industrialized application.

The invention provides a method used for producing hydrogen by taking cellulose as a raw material. The method comprises following steps: 1, microwave alkali digestion; 2, enzyme hydrolysis; 3, dark fermentation hydrogen production; 4, photo fermentation hydrogen production; and 5, purification. The method is high in hydrogen yield, hydrogen production efficiency, substrate utilization ratio, and energy conversion efficiency.

The invention provides a biogas preparation device and method, and belongs to the technical field of energy and chemical industry. The biogas preparation device comprises a wind-solar power generation system, a reaction chamber for generating biogas through microbial electrolysis and dark fermentation, and a double-chamber methane purification chamber for purifying the biogas generated through fermentation in the reaction chamber, wherein the wind-solar power generation system comprises a wind power generator for wind power generation and a photovoltaic module for solar power generation; a cathode electrode and an anode electrode are arranged in the reaction chamber; the double-chamber methane purification chamber comprises a cathode chamber and an anode chamber; and the reaction chamber is connected with the cathode chamber through a pipeline. According to the invention, the sugar refinery wastewater can be recycled, and the preparation efficiency of the biogas and the concentration of methane in the biogas are effectively improved.

The invention provides a tubular multi-line circular light and dark alternating biological hydrogen production method and device. By utilizing the characteristics that anaerobic bacteria have relatively strong matrix degrading capability and photosynthetic bacteria have relatively high raw material conversion rates, photosynthetic and anaerobic bacterium mixed floras are circularly cultured in a light fermentation pipeline and a dark fermentation pipeline to form a biological membrane through enrichment, and biological hydrogen production is carried out according to the modes of light and dark alternation and continuous circulation. The method and the device have the beneficial effects that pretreated biomass raw materials are firstly degraded by the anaerobic bacteria in a dark reaction zone to produce hydrogen, and after being treated, the fermentation liquor after producing hydrogen with the anaerobic bacteria enters a light reaction zone to be further utilized by the photosynthetic bacteria to produce hydrogen, thus increasing the raw material conversion rate and the hydrogen yield; relative to the method for producing hydrogen through dark fermentation with the single anaerobic bacteria, the method for producing hydrogen through mixed fermentation has the advantage of higher raw material utilization rate; relative to the method for producing hydrogen with the single photosynthetic bacteria, the method for producing hydrogen through mixed fermentation has the advantages of better raw material utilization range and higher hydrogen production efficiency, thus obviously improving the efficiency of biological hydrogen production.

The invention provides a low-carbon zero-emission circulating hydrogen preparing device adopting microalgae biomass as fermentation substrate. The device comprises pretreatment equipment, a dark fermentation reactor, solid-liquid separation equipment, a photo fermentation reactor, gas separation equipment and a microalgae growing reactor. According to the device, liquid and gas pollution discharge can be obviously reduced, cyclic utilization and zero emission of carbonic materials can be realized, hydrogen used as clean energy can be prepared, and the hydrogen preparing device is efficient, clean, and environmentally friendly.

The invention relates to the biomass energy utilization technology, aiming at providing a method for preparing hydrogen and methane by adopting cassava dregs preprocessed by methyl morpholine oxide ionic liquid. The method comprises the following steps: grinding cassava dregs, and drying the grinded cassava dregs, thus obtaining a preprocessed material; mixing the preprocessed material with the methyl morpholine oxide ionic liquid, and carrying out pretreatment at 110 DEG C; adding with distilled water, and carrying out stirring, thus obtaining the sediment which serves as the raw material for enzyme hydrolysis; further, adding with deionized water and cellulase; carrying out heating under the sealed state, thus obtaining enzymatic hydrolysate; mixing the enzymatic hydrolysate with deionized water, adding with yeast extract, inoculating dark-fermentation hydrogenogens, and introducing high-purity nitrogen till the dark-fermentation hydrogen producing process is finished; and carrying out sterilization on tail liquid, inoculating activated methanogens, introducing high-purity nitrogen, and carrying out fermentation, thus obtaining methane. According to the method provided by the invention, the yields of hydrogen and methane are obviously improved by adopting the cassava dregs preprocessed by the methyl morpholine oxide ionic liquid; compared with the method for preparing methane by adopting the cassava dregs preprocessed by methyl morpholine oxide ionic liquid, the method for co-preparing hydrogen and methane by adopting the cassava dregs preprocessed by the methyl morpholine oxide ionic liquid provided by the invention has the advantages that the energy exchange efficiency is improved.

The invention provides nickel ferrite nanoparticles and a preparation method and application thereof in promoting hydrogen production, a water hyacinth extracting solution is adopted as a stabilizer and a regulator, a mixed solution of nickel salt and ferric salt is added, continuous stirring reaction is performed, constant-temperature heating reaction is performed in a water bath kettle, and viscous gel is obtained and dried; and calcining, grinding, washing and drying the product to obtain the nickel ferrite nanoparticles. In the preparation process, no extra chemical reagent is added except the precursor nickel salt and the ferric salt, expensive instruments and equipment are not needed, the operation condition is mild, the method is simple and easy to implement, economical and environmentally friendly, and further expanded production and application are facilitated. The prepared nickel ferrite nanoparticles are stable in performance, relatively uniform in particle size and good in biocompatibility, and have the effects of promoting biological hydrogen synthesis and utilizing reducing sugar in straw hydrolysate after being added into a dark fermentation hydrogen production system; and the accumulated hydrogen yield, the glucose utilization rate and the xylose utilization rate obtained under the optimal addition concentration are respectively and obviously improved compared with control treatment.