676results about "Biochemical fuel cells" patented technology

The invention discloses a self-powered biosensor for double-signal detection of sugarcane pokkah boeng disease, the self-powered biosensor comprises an anode, a cathode and an electrolyte, the anode is a CuCo-MOF / AuNPs / GOD biological anode, the cathode is a CuCo-MOF / AuNPs / DNA chain biosensor, and the electrolyte comprises glucose, MB and a PBS buffer solution. According to the self-powered biosensor, analysis is carried out through two non-interfering modes of electrochemical detection and colorimetric detection, and whether a detection result is a false positive signal or not can be effectively verified. An external power supply is not needed, and real-time outdoor detection can be realized. The material source is wide, the assembly process is simple, and accurate and rapid detection can be realized. The method has the advantages of high sensitivity, good selectivity and real-time detection, and can be used for accurately detecting the sugarcane pokkah boeng rot.

A water quality monitoring method. The method comprises: receiving BioElectrochemical System, BES, sensor data indicating an output from at least one BES sensor exposed to a water sample; and receiving data indicating at least one environmental parameter, at least one piece of configuration data for the BES sensor or at least one parameter for a system in which the BES sensor is implemented. The received data is processed according to a calibration algorithm to generate a parameter indicative of organic compound concentration for the water sample. A water quality monitoring device to implement the method may comprise a BES sensor and a processor to implement the calibration algorithm, and optionally one or more further sensors.

The invention belongs to the technical field of fuel cell electrode material preparation, and particularly relates to a carbon electrode material for a polypyrrole modified bio-based fuel cell and a preparation method of the carbon electrode material. Comprising the following steps: modifying rice hull low-temperature carbonized powder by sequentially adopting POSS (Polyhedral Oligomeric Silsesquioxane) containing unipolar functional groups and POSS containing polypolar functional groups to obtain modified low-temperature carbonized powder; and carrying out in-situ polymerization on a pyrrole monomer on the surface of the modified low-temperature carbonized powder, and carrying out high-temperature carbonization and hydrofluoric acid corrosion to obtain the polypyrrole modified bio-based fuel cell carbon electrode material. The polypolar functional group POSS not only is beneficial to the in-situ polymerization reaction of pyrrole, but also can form a continuous macromolecular chain, and a net structure is formed on the surface of the whole material. The effect of the unipolar functional group POSS is to improve the stability of the low-temperature carbonized powder in the high-temperature carbonization process, and more importantly, the unipolar functional group POSS is to provide a channel for subsequent hydrofluoric acid corrosion, etch away non-conductive substances and improve the conductivity and specific surface area of the carbon electrode material.

Disclosed in the present invention are a biomass-derived graded porous asymmetric coordination monatomic carbon-based electrocatalyst, and a preparation method therefor and the use thereof. The preparation method for the biomass-derived graded porous asymmetric coordination monatomic carbon-based electrocatalyst comprises the following steps: (1) stirring and mixing de-alkalized lignin with a transition metal salt, a nitrogen-containing compound and a template, i.e., magnesium oxide, in a solvent until uniform, so as to obtain a suspension, wherein the transition metal comprises iron and zinc; (2) heating, evaporating and drying off the suspension, so as to obtain a lignin / metal ion / nitrogen-containing compound / template mixed precursor; and (3) subjecting the mixed precursor to high-temperature pyrolysis under the protection of an inert gas, cooling same, and then sequentially washing and drying same, so as to obtain a monatomic carbon-based electrocatalyst. The monatomic carbon-based electrocatalyst provided in the present invention has graded pores of micropores, mesopores and macropores, a surface nanosheet and an asymmetric coordination structure of Fe-N3O at the same time, and can be applied to fuel cells and metal-air cells.

The present application relates to an electrochemical system and method for capturing carbon dioxide, regenerating purified carbon dioxide and / or converting the captured carbon dioxide to biomethane, syngas, formic acid, and combinations thereof. More specifically, the present application relates to an electrochemical cell having an air cathode proton or cation exchange membrane and an abiotic or biotic anode wherein captured carbon dioxide is reduced to carbonates that are regenerated as purified carbon dioxide or transferred to another chemical production module. The regenerated carbon dioxide can be purified, compressed, or stored for later use. The present application includes volatile fatty acids sourced from carbohydrate rich waste. The present application also includes the ability to regenerate caustic absorbents used in initial carbon dioxide capture.

The invention relates to the technical field of microbial electrochemistry, in particular to a microbial fuel cell self-driven microbial electrolysis cell system and application thereof. The microbial fuel cell self-driven microbial electrolysis cell system comprises a microbial electrolysis cell (MEC) and a microbial fuel cell (MFC) for supplying power to the MEC; an anode electrode of the MFC is a mixed strain immobilized modified carbon felt electrode, and a cathode electrode of the MFC is a modified carbon felt electrode; an anode electrode and a cathode electrode of the MEC are independently selected from one of a modified carbon felt electrode or a mixed strain immobilized modified carbon felt electrode; the mixed strain is a strain obtained by mixing shewanella and sludge bacteria; the anode electrode of the MEC is connected with the cathode electrode of the MFC, and the anode electrode of the MFC is connected with the cathode electrode of the MEC; the bipolar chambers of the MFC are both in an anaerobic state, and the bipolar chambers of the MEC are both in an anoxic state. The advantages of an MFC-MEC system are fully utilized, external power supply is not needed, the MFC supplies energy to the MEC to promote reverse flow of electrons, and electric energy is utilized in situ.

The invention discloses a device for treating black and odorous water in a constructed wetland in the field of sewage treatment, comprising: a fuel cell assembly comprising a reaction container, a cathode zone, an anode zone and a buffer zone from top to bottom in the reaction container, the cathode zone comprising a catalytic filler, the anode zone comprising an oxidation filler, and the buffer zone comprising a buffer filler; the oxidation filler is a composite material; the water passing assembly is communicated with the anode area and is used for slowly introducing black and odorous water into the anode area, and a plurality of horizontal water outlet pipes are vertically arranged on the reaction container at intervals; the current collection assembly is electrically connected with the anode region and the cathode region; the invention further discloses a manufacturing method. The fuel cell has the beneficial effects that the composite material prepared from the poly (butylene succinate), the manganese oxide and the manganese dioxide is used as the anode filler of the fuel cell, so that the formation of black and odorous substances can be reduced, and the treatment effect of the fuel cell on a black and odorous water body is improved.

The invention discloses a humic acid mediated light-microbial electro-Fenton method for degrading pollutants in sludge. The method comprises the following steps: constructing a double-chamber microbial fuel cell; a biological reaction raw material is added into the anode chamber of the double-chamber microbial fuel cell, and the biological reaction raw material can generate biological reaction to release electrons; adding sludge containing pollutants, humic acid and ferrous ions into a cathode chamber of the double-chamber microbial fuel cell; the anode and the cathode of the microbial fuel cell are connected under the illumination condition, so that a reaction is carried out in the cathode chamber, and pollutants in the sludge are degraded. According to the method, the pollutants in the sludge are degraded by adopting a humic acid mediated light-microbial electro-Fenton method, so that on one hand, a Fenton reaction is promoted to generate a large number of free radicals with relatively strong oxidizing capacity to promote degradation, and on the other hand, growth and metabolism of hosts of the pollutants in the sludge are inhibited to reduce abundance of the pollutants; the synergistic effect of the two can effectively improve the degradation effect.

The invention discloses a constructed wetland microbial fuel cell coupling device based on a novel three-dimensional electrode, and belongs to the technical field of sewage treatment, the device comprises a shell, and a bottom layer, an anode layer, a middle layer and a cathode layer are sequentially arranged in the shell from bottom to top. Wherein the bottom layer is filled with coarse gravels, and the middle layer is filled with fine gravels; the anode layer consists of a plurality of titanium tubes which are horizontally arranged and a titanium mesh for connecting the titanium tubes in parallel; openings are formed in the two ends of the titanium tube, through holes are formed in the titanium tube in an array mode, and the titanium tube is filled with a graphite felt core formed in a curled mode. The graphite felt core body can be used for a conductive material strengthening process and can also be used as a supporting material, and compared with an anode cage structure formed by a stainless steel net, the bearing strength is higher; the resistance of the metallic titanium is lower than that of the stainless steel mesh, so that electron transmission and improvement of electron transfer efficiency are facilitated, and the energy recovery efficiency and pollutant removal efficiency of the constructed wetland microbial fuel cell coupling device can be effectively improved.

The invention relates to the technical field of sewage treatment and resource utilization, in particular to a sludge treatment system with an integrated microbial fuel cell and a treatment method of the sludge treatment system. A first cathode plate matched with the sludge compression chamber is arranged at the other end in the sludge compression chamber in a sliding mode, a driving mechanism used for driving the first cathode plate to be close to or away from the first anode plate is arranged on the sludge compression chamber, and a sludge discharge port is formed in the lower portion of the sludge compression chamber. The chlorine salt and the tannic acid are added into the sludge in the pretreatment barrel, the chlorine salt is used for improving the electrochemical wall breaking effect of the sludge, the tannic acid is used for improving the dewatering performance of the sludge and reducing the hydrothermal dewatering temperature, the chlorine salt and the tannic acid are mixed in the pretreatment barrel and then enter the fuel tank for chemical reaction, electric energy is generated, and electric power is provided for the storage battery; the microorganisms can degrade organic matters in the sludge.

The invention provides a Fenton technology coupled microbial fuel cell, a preparation method and application, and relates to the field of environment, materials and energy, the specific technical scheme is as follows: the Fenton technology coupled microbial fuel cell comprises an anode, a cathode and a catholyte, and is characterized in that the anode is a biological anode, the cathode is an air cathode, and the air cathode is an air cathode; the air cathode comprises an oxygen reduction electrocatalyst and a cathode substrate I, the oxygen reduction electrocatalyst is attached to the cathode substrate I, and the catholyte is common industrial and domestic wastewater. The electro-Fenton technology is introduced into the air cathode of the microbial fuel cell, the cathode fully utilizes electrons generated by metabolism of a biological anode to carry out an oxygen reduction process in the presence of air, so that hydrogen peroxide is generated in situ and is activated into active species such as hydroxyl free radicals through a Fenton catalyst, and the hydrogen peroxide is converted into hydrogen peroxide. Therefore, the purpose of degrading complex pollutants with low energy consumption is achieved.

The invention discloses a nitrogen-doped carbon nanotube hollow polyhedral material, a bifunctional electrode based on the material and application of the nitrogen-doped carbon nanotube hollow polyhedral material. The preparation method comprises the following steps: preparing a precursor material ZIF-8 (at) ZIF-67 with a core-shell structure by taking ZIF-8 with a dodecahedron structure as a template; wherein the dosage ratio of the ZIF-8 to the 2-methylimidazole to the cobalt salt is (0.2-0.5) g: (5-10) g: (1.7-34) mmol; and performing high-temperature pyrolysis on the precursor material in inert gas, and performing acid pickling and washing to be neutral to obtain the composite material. The difunctional electrode is an electrode for modifying the material, can be used for detecting H2O2 and glucose, or can be used as a cathode or an anode of an enzyme biological fuel cell. The difunctional electrode has the advantages of low detection limit, wide linear range and high sensitivity when being used for detecting H2O2 and glucose. The maximum open-circuit voltage of the constructed enzyme biological fuel cell is 0.423 V, and the maximum power density is 3.70 [mu] W / cm <-2 >. The electrode material disclosed by the invention has good performance and low cost, and has a wide prospect in application of electrochemical sensing and battery equipment.

The invention discloses a microbial fuel cell coupling system for industrial wastewater treatment and energy regeneration, and the system comprises a wastewater treatment and energy conversion module which is used for introducing industrial wastewater into a microbial fuel cell, realizing efficient decomposition of organic matters under the metabolism effect of microbiota in an anode chamber, generating purified water reaching the standard, and recovering clean energy; the intelligent parameter control module is used for dynamically adjusting operation parameters of each unit through an MLP model; and the dynamic monitoring feedback module carries a high-precision sensor array and an internet-of-things transmission device, acquires parameters in the fuel cell in real time, provides a predictive regulation and control strategy for the system by applying an MLP model, and forms closed-loop optimization through an energy-material-information three-flow coupling mechanism. Through cooperative work of all the modules, efficient and stable operation of the system is achieved, the prospect in the field of industrial wastewater treatment is wide, the resource utilization efficiency can be remarkably improved, and the cost is reduced.

The invention belongs to the technical field of microbial fuel cells, and particularly relates to a microbial fuel cell, a carbon-based anode and a preparation method of the carbon-based anode. Preparing a polyaniline / dopamine modifier from an aniline monomer and dopamine hydrochloride, and modifying the polyaniline / dopamine modifier on a carbon felt electrode to form a PANI-DA / CF anode; the preparation method comprises the following steps: preparing flower-like Fe2O3 nanoparticles by adopting a hydrothermal method, and electrochemically depositing the flower-like Fe2O3 nanoparticles on a PANI-DA / CF electrode to prepare a Fe2O3 / PANI-DA / CF anode; according to the microbial fuel cell disclosed by the invention, the maximum power density of the phenol wastewater anolyte reaches 4594 + / -97 mW / m < 2 > and is improved by 83.1% compared with that of a carbon felt anode MFC (Microbial Fuel Cell); after operation for 96 hours, the phenol degradation rate reaches 99.4%; and the electricity generation performance is still kept stable after three months of operation. The Fe2O3 / PANI-DA / CF anode provided by the invention improves the power density output of the microbial fuel cell and the wastewater treatment efficiency.

The utility model relates to a polluted landscape water body treatment device, including coupling type microbial fuel cell and external circuit, coupling type microbial fuel cell includes cell cathode, fixed ring, gasbag, cell anode, PVC flexible pipe, proton exchange membrane, PVC flexible pipe is filled with electrolyte, the cell anode is planted with submerged plant, the proton exchange membrane is planted on the PVC flexible pipe. And the external circuit comprises a light-emitting circuit, a resistance circuit and a heating circuit which are connected in parallel between the battery cathode and the battery anode. Electric energy generated by the microbial fuel cell is used for providing power for photocatalytic oxidation, magnetic flocculation recovery and water body temperature rise in the treatment process, no external power source is arranged, the water body treatment capacity is greatly improved, meanwhile, the later operation and maintenance cost is reduced, aquatic plant and microbial treatment is introduced into the system, and the water body treatment efficiency is improved. A mutual promotion effect can be achieved with the microbial fuel cell, so that the power generation capacity of the cell and the processing capacity of the system are improved.

To provide a novel microorganism having higher current generating ability, a microbial fuel cell composition and microbial electrolysis cell composition comprising the microorganism, as well as a microbial electrolysis cell and microbial electrolysis cell using the microorganism.SOLUTION: Provided are: a microorganism, a Geobacter sp. strain 60473; a microbial fuel cell composition comprising the Geobacter sp. strain 60473 and an organic substance; a microbial fuel cell in which the Geobacter sp. strain 60473 is used to decompose the organic substance as a fuel; a microbial electrolysis cell composition comprising the Geobacter sp. strain 60473 and the organic substance; and a microbial electrolysis cell in which the Geobacter sp. strain 60473 is used to decompose the organic substance as the fuel.SELECTED DRAWING: None

The invention provides a pulsating anaerobic microorganism multi-phase fluidized bed hydrogen-electricity combined supply system and a use method, and belongs to the technical field of sewage treatment. The system comprises a constant-temperature liquid storage tank, an anode chamber, a hydrogen production reaction chamber and other key components, and smooth flowing of liquid and gas is achieved through pipeline connection. In order to improve the reaction efficiency, key components such as a distributor, a particle confinement network and a bubble generator are arranged in the system. According to the system, microorganisms such as escherichia coli and shewanella are inoculated to form an efficient biological membrane, and a nano Fe3O4 microbial membrane is added. The system combines pulsation and stable liquid flow, efficiency is improved, and energy consumption is reduced. And the electric energy of the microbial fuel cell is introduced into the hydrogen production reaction chamber and the super capacitor, so that the electric energy is utilized and recycled. And meanwhile, the monitoring computer and the water quality detector are used for monitoring the generated voltage and water quality in real time. Chemical energy in sewage can be fully utilized, the energy conversion efficiency is improved, application with higher requirements is met, and meanwhile the reaction energy consumption is low.

The invention belongs to the technical field of new energy materials and bioelectrochemistry, and particularly relates to an iron-based multi-metal sulfide composite carbon anode material and a preparation method of the iron-based multi-metal sulfide composite carbon anode material. The preparation method is simple in process flow, convenient to operate, easy for large-scale production, wide in raw material source, low in cost and environment-friendly, and can be used for preparing the three-dimensional porous carbon matrix which is formed by foaming and carbonizing polyvinylpyrrolidone and is provided with interconnected carbon nanosheets. Through reasonable composition design and structural regulation, and optimization of metal ratio and vulcanization conditions, accurate regulation of material performance can be realized, electrochemical performance and biocompatibility are significantly improved, electricity generation performance and operation stability of a microbial fuel cell are improved, and good industrial application prospects are achieved.

Described are enzyme fuel cells and batteries comprising an anode with a multicopper oxidase enzyme immobilized with a phenolic substrate.

The invention relates to a microbial fuel cell and application thereof, in particular to a photocatalytic / microbial coupled anode microbial fuel cell and application thereof in enhanced degradation of sulfamethoxazole. Aiming at the degradation treatment of typical refractory antibiotics such as sulfamethoxazole, the invention provides a photocatalytic / microbial coupled anode microbial fuel cell which can induce a photo-generated hole generated by a photocatalyst through a biological electric field generated by microbial degradation of pollutants, thereby promoting the separation of photo-generated electron hole pairs of the photocatalyst, and further improving the degradation efficiency of the antibiotics. The problem that photo-generated electron hole pairs of a photocatalyst are easy to compound is solved. Meanwhile, due to the existence of the photocatalyst, the electron transfer between the anode electroactive microorganisms and the anode can be improved through the generation of photo-generated holes, and the extracellular electron transfer of the microorganisms is enhanced. When being used for degrading sulfamethoxazole, the photocatalytic / microbial coupled anode microbial fuel cell disclosed by the invention has an extremely important scientific research value and a wide practical application prospect.

The invention discloses a nitrogen / boron-doped non-noble metal-based material, a synthesis method thereof and application of the nitrogen / boron-doped non-noble metal-based material in a microbial fuel cell. According to the method, a carbon fiber brush is used as a substrate, urea and sodium borohydride are used as a nitrogen source and a carbon source respectively, and the nitrogen / boron doped non-noble metal-based material is synthesized through a hydrothermal method. The electrostatic interaction between graphene oxide and FeCoNi-LDH is utilized to realize tight combination, so that FeCoNi-LDH nanosheets are uniformly dispersed on the surface of the carbon fiber brush, and the problems that LDHs are easy to agglomerate and poor in conductivity are solved; in the boron / nitrogen co-doping process, an efficient electron transmission channel is constructed by adjusting the electronic structure of the composite material, and the material is endowed with excellent electron transmission capability and oxygen reduction performance. In an MFC (Microbial Fuel Cell) test constructed by taking a nitrogen / boron-doped non-noble metal-based material as a cathode, the output voltage and the maximum power density are several times of those of commercial Pt / C and a blank carbon fiber brush, and the carbon fiber brush has a wide application prospect in the field of microbial fuel cells.

The utility model discloses a garbage biogas gasification treatment device capable of generating electricity, which relates to the technical field of garbage treatment equipment and comprises a reaction chamber, two conductive parts and a resistor or an energy storage element, a low-oxygen or oxygen-free environment can be kept in the reaction chamber, and a liquid inlet, a liquid outlet, a gas outlet and two fixing ports are arranged on the reaction chamber; the two conductive parts respectively extend into the reaction chamber through the two fixing ports to form two electrodes; the resistor or the energy storage element is arranged outside the reaction chamber; the resistor or the energy storage element is electrically connected with the two conductive pieces and enables the two electrodes to respectively form an anode and a cathode. According to the device, a microbial fuel cell and an anaerobic fermentation process are integrated in one set of device, so that the microbial fuel cell and the anaerobic fermentation process are highly and effectively integrated, the removal rate of organic matters in garbage is high, the occupied space is small, the proportion of CH4 in biogas is high, and electric energy is generated.

The invention discloses a high-sensitivity oxygen-resistant anode MFC (Microbial Fuel Cell) sensor for biotoxicity detection and a preparation method thereof. The sensor comprises a positive electrode, a negative electrode, a positive electrode chamber, a negative electrode chamber, a diaphragm, a current loop and a data acquisition system, a double-layer biological membrane is formed on the positive electrode through aerobic domestication, the outer layer is an aerobic bacteria layer, and the inner layer is an anaerobic electroactive bacteria layer. The aerobic bacteria on the outer layer consume dissolved oxygen and provide a stable environment for the anaerobic bacteria on the inner layer, so that the oxygen resistance and the operation stability of the sensor are remarkably improved. When the toxicity of a to-be-detected sample is detected, the biotoxicity of the sample is represented through the electrogenesis inhibition rate of the sensor. Experiments show that the response sensitivity of the sensor to toxic substances (such as Cu (II)) is obviously higher than that of a traditional anaerobic MFC sensor, and the sensor shows excellent anti-interference capability under different dissolved oxygen conditions. The method is suitable for rapid detection of biotoxicity of water and environmental samples, and has the characteristics of high sensitivity, strong oxidation interference resistance and good stability.

The invention belongs to the technical field of biological fuel cells, and particularly relates to an enzyme biological fuel cell system for detecting sugarcane pokkah boeng disease through three signals as well as a preparation method and application of the enzyme biological fuel cell system. The enzyme biological fuel cell system comprises an enzyme biological fuel cell and a photothermal conversion solution TiO2 (at) Au, the enzyme biological fuel cell comprises a biological anode, a biological cathode and an electrolyte, the biological anode is MWNT (at) ZIF-8 / AuNPs / GOD, and the biological cathode is an MWNT (at) ZIF-8 / AuNPs / DNA chain. According to the invention, false positive signals of detection results can be effectively calibrated; through combined use of three detection modes, high sensitivity, selectivity and real-time monitoring capability can be realized, and the method can be used for accurate detection of the sugarcane pokkah boeng rot.

The present invention relates to a method and system for producing adenosine triphosphate (ATP) through an acid / aldehyde-ATP (AAA) cycle in an electrochemical cell.

The invention discloses a method for enhanced removal of VOCs (volatile organic compounds) and synchronous power generation by a biological filter bed coupled microbial fuel cell system. According to the method, VOCs are introduced into a cathode chamber of a microbial fuel cell and then upwards penetrate through a filler layer of a fungus-bacterium coupled biological filter bed, in the stage, hydrophobic VOCs are adsorbed by fungal hyphae and then are aerobically degraded by bacteria to generate metabolic intermediates, hydrophilic VOCs can be directly degraded by aerobic bacteria, the purified VOCs are discharged from the top, and the purified VOCs are discharged from the bottom of the microbial fuel cell. Hydrophilic VOCs and metabolic intermediates which are not degraded in time can enter an anode chamber of the microbial fuel cell along with the nutrient solution, and the hydrophilic VOCs and the metabolic intermediates are further degraded as power generation substrates and generate electrons (e <->). According to the invention, the fungus-bacteria mixed biological filter bed is coupled with the microbial fuel cell system for treatment, so that enhanced removal is realized, and high-efficiency power generation is also realized.

An electrode comprising a self-supporting porous structure of expanded graphite and an electrical connector. Method for manufacturing a self-supporting porous structure of expanded graphite, said method comprising the following steps: preparing a suspension of expanded graphite particles in a liquid; sonicating said suspension of graphite particles by applying ultrasound, to obtain a homogenized suspension; and filtering said homogenized suspension under pressure to obtain an expanded porous structure which is at least partially and preferably substantially dry.

The invention discloses a biomass-derived graded porous asymmetric coordination monatomic carbon-based electrocatalyst as well as a preparation method and application thereof. A preparation method of a biomass-derived graded porous asymmetric coordination monatomic carbon-based electrocatalyst comprises the following steps: (1) dealkalized lignin, transition metal salt, a nitrogen-containing compound and a template agent magnesium oxide are stirred and mixed uniformly in a solvent to obtain a suspension, and the transition metal comprises iron and zinc; (2) heating, evaporating and drying the turbid liquid to obtain a lignin / metal ion / nitrogen-containing compound / template agent mixed precursor; and (3) performing high-temperature pyrolysis on the mixed precursor under the protection of inert gas, cooling, and sequentially washing and drying to obtain the monatomic carbon-based electrocatalyst. The monatomic carbon-based electrocatalyst provided by the invention has hierarchical pores of micropores, mesopores and macropores, a surface nanosheet and an asymmetric coordination structure of Fe-N3O at the same time, and can be applied to fuel cells and metal-air cells.

The invention relates to the technical field of microbial fuel biological cells, in particular to an efficient electrogenesis microbial fuel cell as well as preparation and application thereof. The method comprises the following steps: firstly, carrying out anaerobic domestication on current-producing bacteria in active sludge subjected to sieving and impurity removal to obtain mixed current-producing bacteria; then putting the mixed current-producing bacteria into an anaerobic basic culture medium containing phenazine-1-carboxylic acid for enrichment culture to obtain enriched mixed current-producing bacteria; and finally, putting the enriched mixed electrogenesis bacteria and the anolyte into an anode chamber of a microbial fuel cell double-chamber reactor, and then adding the catholyte into a cathode chamber to obtain the microbial fuel cell capable of efficiently generating electricity. Wherein in the anaerobic domestication process, the salt tolerance of the current-producing bacteria in the activated sludge is enhanced by utilizing the stress pressure of jump salinity. According to the method, the electricity-producing bacteria are cultured by adopting a jumping type salinity gradient stimulation method, and the obtained MFC is suitable for application scenes of chemical high-salinity wastewater, so that the method has a relatively good industrialization prospect.

The invention discloses a preparation method of a layered double hydroxide combined cobalt disulfide and nickel tetrasulfide combined multi-walled carbon nanotube catalyst, which comprises the following steps: firstly, preparing cobalt disulfide and nickel tetrasulfide combined multi-walled carbon nanotubes through a two-step hydrothermal method; and then successfully preparing the ferro-nickel layered double hydroxide and nickel dicobalt tetrasulfide combined multi-walled carbon nanotube. The invention also discloses an application of the ferro-nickel layered double hydroxide and nickel dicobalt tetrasulfide combined multi-walled carbon nanotube as a microbial fuel cell cathode catalyst. The prepared material has higher porosity, more reaction sites, better electrochemical stability and higher conductivity, so that when the material is used as a cathode catalyst of the microbial fuel cell, the efficiency of electric ion flow is improved, the stability of electrode circulation and the power generation efficiency are ensured, and the performance of the microbial fuel cell is improved.