The present disclosure relates to a
working electrode of a glucose monitoring probe that reduces interference, comprising a base layer, a sensing layer, a
semipermeable membrane, and a biocompatible membrane, wherein the base layer is disposed on a flexible substrate; the sensing layer is formed on the base layer by
coating a sensing layer
reagent, which is capable of chemically reacting with glucose in
blood or tissue fluid, wherein the sensing layer
reagent comprises a
redox polymer, a glucose
enzyme, a
carbon nanotube, and a cross-linking agent, wherein amino groups are added to the carbon nanotubes to tightly form a
covalent bond between the
redox polymer and the carbon nanotubes and bind to the glucose
enzyme, thereby reducing the working
voltage required for the
working electrode under the catalytic action of the carbon nanotubes, wherein the glucose
enzyme is
glucose oxidase; the
semipermeable membrane is formed on the sensing layer to control the passage rate of glucose molecules; and the biocompatible membrane is formed on the
semipermeable membrane. According to the present disclosure, a
working electrode of a glucose monitoring probe that can reduce interference is provided.