265 results about "Electrochemical biosensor" patented technology

A method for circumscribing an insulating barrier region around a singular conductive microneedle structure or plurality of conductive microneedle structures adhered to a fixed substrate for the purpose of spatially defining a conduit for the routing of an electrical signal from the surface of said microneedle or microneedles to the posterior surface of the substrate is disclosed herein. A microneedle-based electrochemical biosensors structure comprises a substrate, a microneedle biosensor, a primary electrically conductive element, a secondary electrically conductive element and an electrically insulative annular barrier.

The invention provides an integrated electrochemical biosensor capable of detecting double miRNA with high sensitivity, and the integrated electrochemical biosensor can simultaneously detect double miRNA markers with high sensitivity through self-assembly of a Y-shaped capture probe, preparation of an Au / TiO2-FTO array electrode, catalytic hairpin amplification (CHA) and construction of a micro-droplet chip electrochemical biosensor. The advantages of equalization of double-target miRNA capture probe sites, excellent electrode performance, reliable signal amplification, low sample consumption and the like are integrated, the linear range of miRNA21 and miRNA155 detection is 10 fM to 100 nM, and the detection limits are 5.00 fM and 5.17 fM respectively. By detecting the miRNA21 and miRNA155 double markers, the accuracy of breast cancer diagnosis is up to 98%, and the recognition sequences at the two ends of the Y stent probe are modified, so that the sensor can be used for diagnosing other miRNA marker related diseases. The integrated micro-droplet chip electrochemical biosensor constructed by the invention has a remarkable clinical diagnosis value and a prominent practical application prospect.

The present invention relates to: an electrochemical biosensor capable of effectively and sensitively detecting alfuzosin; an electrode applied thereto; a metal-organic framework (MOF) which includes a zinc / cobalt oxide and is applied in manufacturing the electrode; and methods for manufacturing same.

The invention discloses a paper-based electrode based on liquid metal assisted laser-induced graphene and a preparation method thereof.The paper-based electrode comprises a substrate layer, a functional layer, a conductive layer and a hydrophobic layer which are sequentially arranged, the substrate layer is made of paper, the functional layer is formed by coating liquid metal nanoparticles, and the conductive layer is formed by coating liquid metal nanoparticles. The conductive layer is an electrode formed by a laser-induced graphene technology, and the hydrophobic layer is a fluid control area formed by wax packaging. According to the invention, paper is used as a substrate, liquid metal is combined with a laser-induced graphene technology, surface modification is carried out through eutectic gallium indium (EGaIn), polyimide (PI) and N-methyl pyrrolidone (NMP) composite slurry, and then a conductive electrode pattern is formed on the surface of the paper substrate by using a laser-induced graphene (LIG) technology. The method has the advantages of simple preparation, low cost, environmental friendliness and the like, and the prepared paper-based electrochemical biosensor has excellent conductivity, mechanical flexibility and electrochemical activity.

The invention provides an electrochemical biosensor for detecting mycoplasma pneumoniae, the core of the electrochemical biosensor comprises a specific capture probe, and the nucleotide sequence of the specific capture probe is a complementary sequence of a 23S-5S rDNA spacer sequence (ITS) of the mycoplasma pneumoniae. In the preparation link, the porous membrane is used as a high-efficiency electron transmission channel, the process is remarkably simplified, and the reaction efficiency is improved. By virtue of a capture probe taking 23S-5S rDNA ITS as a target, the sensor is endowed with excellent specificity and high sensitivity, so that the sensor has excellent performance in complex biological sample detection, can accurately and efficiently recognize a target object and effectively avoids interference of other substances. The electrochemical biosensor is easy and convenient to operate and high in response speed, has wide application prospects in the fields of clinical diagnosis and rapid disease screening, can provide key technical support for timely diagnosis of mycoplasma pneumoniae infection, greatly meets the urgent requirements for rapid and accurate detection in clinical and practical application, and has good application prospects. The development process of a biological detection technology in the mycoplasma pneumoniae detection direction is powerfully promoted.

The invention provides an electrochemical biosensor based on ultrasonic bidirectional driving and a detection method, and belongs to the technical field of biosensing. The sensor comprises a porous membrane and an ultrasonic bidirectional driving unit, and a probe capable of specifically capturing a target object is fixed in a pore channel of the porous membrane; the ultrasonic bidirectional driving unit is composed of a first ultrasonic transducer and a second ultrasonic transducer which are symmetrically arranged on the two sides of the porous membrane, and can synchronously execute reverse power gradient change to form alternately changing pressure difference on the two sides of the membrane so as to drive liquid to repeatedly penetrate through the porous membrane. The detection method comprises the steps of to-be-detected liquid injection and ultrasonic bidirectional driving, ultrasonic auxiliary cleaning and electrochemical detection. The problems of insufficient probe utilization rate, low mass transfer efficiency and non-specific adsorption of a traditional passive diffusion mechanism are solved by utilizing bidirectional ultrasonic active driving force, the detection sensitivity, speed and signal-to-noise ratio are remarkably improved, and the method is suitable for rapid and high-sensitivity quantitative analysis of various analytes.

A method for circumscribing an insulating barrier region around a singular conductive microneedle structure or plurality of conductive microneedle structures adhered to a fixed substrate for the purpose of spatially defining a conduit for the routing of an electrical signal from the surface of said microneedle or microneedles to the posterior surface of the substrate is disclosed herein. A microneedle-based electrochemical biosensors structure comprises a substrate, a microneedle biosensor, a primary electrically conductive element, a secondary electrically conductive element and an electrically insulative annular barrier.

The invention discloses a preparation method of an electrochemical biosensor based on neuraminidase, and relates to the field of electrochemical sensors. The method comprises the following steps: preparing N-Gr / MWCNTs, preparing N-Gr / MWCNTs (at) GCE, preparing Ppy / N-Gr / MWCNTs (at) GCE, and preparing NA / Ppy / N-Gr / MWCNTs (at) GCE. According to the electrochemical biosensor based on neuraminidase, MWCNTs and N-Gr are compounded to form an N-Gr / MWCNTs composite material, high-conductivity polypyrrole is polymerized on the N-Gr / MWCNTs composite material through pyrrole, the biosensor without a mediator is prepared, electrons can be directly transmitted between an electrode and a bioactive substance, and the electrochemical biosensor can be used for detecting neuraminidase. The response performance of the sensor is obviously improved; the electrochemical biosensor is based on the NA inhibition principle, is mainly used for rapidly screening antiviral components in traditional Chinese medicines, can realize rapid screening and evaluation of the antiviral components in the traditional Chinese medicines, belongs to a convenient, efficient and low-cost means, and also provides scientific basis and technical support for discovery and evaluation of active components.

The present invention relates to an electrode for an electrochemical biosensor for selectively detecting dopamine, an electrochemical biosensor comprising same, and a manufacturing method therefor and, specifically, to an electrode for an electrochemical biosensor for selectively detecting dopamine, an electrochemical biosensor comprising same, and a manufacturing method therefor, wherein the electrode has a lower trace detection limit compared with an existing dopamine detection sensor and exhibits superb stability, reproducibility, and selectivity.

The invention discloses an electrochemical biosensing system for detecting BVDV (bovine viral diarrhea virus) based on CRISPR-Cas13a (clustered regularly interspaced short palindromic repeats). The electrochemical biosensing system comprises an electrochemical biosensor and a CRISPR-Cas13a system, the electrochemical biosensor comprises a working electrode, and the surface of the working electrode is modified with an MB-RNA probe; one end of the MB-RNA probe is modified with sulfydryl, the other end of the MB-RNA probe is modified with signal molecules, and the MB-RNA probe is connected with the working electrode through an Au-S bond; the CRISPR-Cas13a system comprises a crRNA (Complementary Ribonucleic Acid) group and Cas13a; and the crRNA group comprises at least one of BVDV (bovine viral diarrhea virus)-crRNA 2, BVDV-crRNA 7, BVDV-crRNA 8, BVDV-crRNA 11, BVDV-crRNA 12 and BVDV-crRNA 14. The electrochemical biosensing system disclosed by the invention has extremely high clinical sensitivity and clinical specificity on the BVDV virus.

The invention discloses an electrochemical biosensor of a light-driven nanopore channel and application of the electrochemical biosensor. According to the sensor, a photoresponse nanopore membrane is constructed, an outer surface aptamer probe is combined, asymmetric photothermal gradient driven ion transmission induced by near-infrared light is utilized, and ultra-sensitive detection on a target object is realized by regulating and controlling the charge density of the outer surface. According to the electrochemical biosensor provided by the invention, traditional voltage driving is replaced by asymmetric near-infrared light driving, so that the electrochemical biosensor has a relatively wide detection range and an extremely low detection limit, and high-sensitivity and specific detection on a target object can be realized. The electrochemical biosensor can be used for detecting microcystin-LR (MC-LR), the detection limit reaches 1 * 10 <-7 > mu g / L, the sensitivity is improved by 5 orders of magnitude compared with that of a traditional voltage-driven sensor, and the electrochemical biosensor can be applied to trace pollutant detection in the fields of environmental monitoring, food safety and the like.

The invention relates to the technical field of electrochemical analysis and detection, in particular to an electrochemical biosensor based on CRISPR / Cas12a as well as a preparation method, a detection method and application of the electrochemical biosensor. An electrochemical biosensor based on CRISPR / Cas12a comprises a Cas12a-crRNA detection system, an APT-ActDNA adaptation system and an electrochemical aptamer sensor, and when a target analyte exists, the Cas12a-crRNA detection system and the APT-ActDNA adaptation system are matched with each other to react with the target analyte, so that the electrochemical aptamer sensor generates an electrochemical signal. According to the technical scheme, the problems of specific recognition and detection of amyloid beta peptide (Abeta) in a complex biological sample are solved, the preparation method is simple, the cost is low, and the detection method is high in sensitivity and accuracy.

The invention discloses ultrasensitive detection of vibrio parahaemolyticus by a CRISPR (clustered regularly interspaced short palindromic repeats) electrochemical biosensor based on PCR (polymerase chain reaction) and a hairpin DNA (deoxyribonucleic acid) probe, which comprises the following steps: preparing a gold nanoelectrode, modifying the gold nanoelectrode by using the hairpin DNA probe, performing polymerase chain reaction amplification to obtain a PCR amplification product, adding the PCR amplification product into a CRISPR system, uniformly oscillating, and detecting the vibrio parahaemolyticus by using the CRISPR electrochemical biosensor. Placing in a 37 DEG C constant-temperature metal bath for pre-reaction for 10 minutes, and dropwise adding the obtained reaction system to the surface of the hairpin DNA probe modified gold nanoelectrode. The method disclosed by the invention is high in overall repeatability and stability, the modification density of the surface of the electrode can be reduced, the steric hindrance effect is further reduced, the trans-cutting efficiency is improved, and in actual detection, the method shows remarkable sensitivity, is high in specificity, short in time consumption, low in cost and simple and convenient to operate, and can be applied to field detection.

The invention discloses an electrochemical biosensor for detecting mycobacterium tuberculosis. The sensor takes a porous membrane as a base material, the inner wall of a pore channel of the porous membrane is co-modified with a polydopamine (PDA) and conductive carbon material composite layer, and an amino-modified specific capture probe based on a mycobacterium tuberculosis ESAT-6 gene is fixed on a modification layer through covalent binding. When a target gene is captured by a probe, the volume effect of the target gene causes blockage of a porous channel of the porous membrane, so that electrochemical signals (such as current) of the sensor are changed, and high-sensitivity and specific detection of mycobacterium tuberculosis is realized. According to the invention, a porous structure, biological probe specific fixation and an electrochemical signal conversion technology are combined, and a novel efficient tool is provided for rapid diagnosis of tuberculosis.

Electrochemical biosensors are devices that convert biological signals into electrical signals for detection. Described herein are automation-compatible high-throughput electrochemical sensing devices and systems that form a high-throughput electrochemical sensing platform. In certain embodiments, the platform includes: a sensor plate assembly with a sensor board and multi-well plate, where each well contains at least one three- electrode assembly (e.g., a working electrode, a reference electrode, and a counter electrode) with a conductive antifouling coating; a control board for detecting signals from each of the multiple electrode assemblies; an optional heater and / or temperature controller for the sensor board; and an interconnect assembly to facilitate replacement of the sensor board.

The invention provides construction of an electrochemical biosensor based on a fucoidin hydrogel microneedle and application of the electrochemical biosensor in online monitoring of uric acid, and relates to the technical field of biosensors. According to the micro-needle, Gantrez S-97 and PEG are subjected to copolymerization crosslinking to form a hydrogel main body, fucoidin is introduced for blending modification, a three-dimensional network structure is constructed by utilizing a high-temperature esterification reaction for regulating and controlling pH, and the super-expansion hydrogel micro-needle with high swelling property and good biocompatibility is formed. The microneedle can rapidly collect a skin interstitial fluid sample, and then through a screen printing electrode (SPE) platform, carboxylated MWCNTs are used for modifying an electrode and immobilizing uricase to construct an enzyme sensor. Hydrogen peroxide generated by catalyzing uric acid on the surface of the electrode participates in electrochemical reaction, and an output signal is used for real-time detection of uric acid concentration. The device integrates minimally invasive sampling and high-sensitivity electrochemical detection, has the advantages of quick response, simplicity and convenience in operation, good biocompatibility and the like, and is suitable for application scenes of continuous monitoring of uric acid, chronic disease management and the like.

The invention designs a preparation method of an electrochemical sensor for detecting acrylamide based on a Ce-MOF and AuPd nanoflower composite material. The advantages of large surface area and good stability of Ce-MOF are utilized to load a large amount of AuPd NFs, and the composite material Ce-MOF (at) AuPd NFs with high conductivity is obtained. The preparation method comprises the following steps: modifying an electrode by virtue of a physical adsorption effect, fixing a triple-helix molecular switch THMS on the electrode by virtue of a gold-sulfur bond and a palladium-sulfur bond, when acrylamide exists, combining acrylamide with an aptamer, forming DNAzyme by virtue of single-stranded CDNA1 and single-stranded CDNA2, and driving a DNA walker amplification strategy in the presence of Mg < 2 + >, so that a hairpin HP1 is sheared, and a large amount of single-stranded S1 is obtained. According to the method, S1, signal tags combined on an electrode are reduced and signals are reduced by destroying a triple helix structure, so that acrylamide detection is realized. The electrochemical biosensor constructed by the method disclosed by the invention has relatively high specificity and relatively high sensitivity.

The invention discloses a preparation method and application of an electrochemical biosensor for dynamically monitoring Cyt c in tumor cells based on fast scanning voltammetry. The preparation method is characterized by comprising the following steps: 1) preparing a PtNE nano electrode; (2) preparing the PtNE / Apt nano electrochemical biosensor; and (3) accurately puncturing the PtNE / Apt electrode into cytoplasm of a single Hela cell through a three-dimensional micro-control system, so that Apt and intracellular Cyt c are specifically combined and subjected to conformational change, and 3 '-end modified Fc is close to the surface of the electrode, thereby generating a remarkable electrochemical signal. The change of Fc redox peak current is dynamically monitored through fast scanning voltammetry, the quantitative relation between the Cyt c concentration and the peak current is established, and the relation between the Cyt c concentration and the cell activity is monitored according to the quantitative relation. The device has the advantages of high sensitivity, excellent anti-interference capability and remarkable signal amplification effect.

This disclosure provides a wearable electrochemical biosensor for detecting at least one target analyte in interstitial fluid of a subject, comprising, a microneedle array comprising a highly- crosslinked hydrogel polymer; at least one electrochemical chip comprising at least one transducer surface configured for generating a signal in the presence of the at least one target analyte; and a low crosslinked hydrogel polymer, wherein the low crosslinked hydrogel polymer is configured to couple the microneedle array with the at least one transducer surface of the electrochemical chip. Methods of making and use thereof are also disclosed.

The invention belongs to the technical field of electrochemical biosensors, and particularly relates to an electrochemical biosensor based on probe strand displacement reaction. According to the sensor, a capture probe is fixed in a nuclear track membrane and is specifically combined with a detection probe, and when target nucleic acid exists, on the basis of the Toehold mediated strand displacement reaction principle, the target nucleic acid replaces the detection probe, and electrode impedance change is triggered to achieve detection. Particularly, reversible replacement between the target nucleic acid and the detection probe is realized by designing double Toehold structural domains. The electrochemical biosensor disclosed by the invention is simple, convenient, rapid and high in specificity, and a new thought is provided for constructing a high-sensitivity and reusable electrochemical biosensing platform.

This invention discloses an amplification-free electrochemical biosensor and method based on CRISPR-Cas13a. The sensor includes an electrochemical biosensor substrate, a dual-channel RNA reporter molecule, a CRISPR-Cas13a reaction system, and a dual-channel differential signal processing module. The working electrode surface is immobilized with a stem-loop structured first reporter molecule and a linear second reporter molecule loaded with gold nanoparticles, labeled with methylene blue and ferrocene signal molecules respectively, and immobilized with a ferrocene-specific capture probe. When target RNA is present, Cas13a is activated and non-specifically cleaves the reporter molecule, reducing the methylene blue signal and enriching and enhancing the ferrocene signal. Background self-calibration is achieved through dual-channel differential ratio calculation. This invention integrates signal attenuation and signal enhancement modes, offering advantages such as nucleic acid amplification-free operation, fast detection speed, high sensitivity, and strong anti-interference capability.

The invention discloses an efficient electrochemical biosensor for myocardial infarction detection. The invention provides a substrate material / PtAg nano composite material and application thereof in construction of an electrochemical immunosensor. The substrate material / PtAg nano composite material is characterized in that the substrate material is selected from MXene or p-COF. The method has the effects of low detection limit and high sensitivity.

The invention discloses an aptamer electrochemical biosensor for estradiol detection as well as a preparation method and application of the aptamer electrochemical biosensor. The aptamer electrochemical biosensor is obtained by growing AuNPs particles on the surface of MoS2 to prepare a MoS2-AuNPs composite material, fixing the composite material on the surface of an electrode through chitosan, hybridizing aptamer probes P1 and P2, and self-assembling the aptamer probes P1 and P2 on the surface of the electrode through Au-S bonds. The sensor can be used for in-situ detection of hormone factor estradiol in sweat, can be applied to non-invasive monitoring equipment, and provides reliable and timely monitoring data.

The application belongs to the field of electrochemical biosensors, and more particularly relates to a protein molecule labeling method for constructing a high-performance electrochemical sensor. In the application, organic ligands on the surface of sulfide colloidal quantum dots are replaced with water-soluble ligands through liquid-phase ligand replacement, and then mixed with a carboxyl activator to activate the carboxyl groups of the water-soluble ligands, followed by uniform mixing with protein molecules, so that the protein molecules are effectively labeled on the quantum dots. The quantum dots prepared in the application, which have water-soluble ligands on the surface and are labeled with protein molecules, are coated on the working electrode of a planar three-electrode system through a one-step method, and after drying, an electrochemical biosensor with a wide detection range, high response sensitivity and strong anti-interference performance is prepared.

The invention provides an electrochemical biosensor used for alpha-synuclein detection. The sensor comprises a magnetic particle capture probe coated by a monoclonal antibody, a SiO2 nanoparticle signal probe for detecting antibody modification and a nano porous membrane loaded glassy carbon electrode modified by graphene. During detection, alpha-synuclein is specifically combined with the monoclonal antibody-coated magnetic particle capture probe and the detection antibody-modified cadmium-doped SiO2 nanoparticle signal probe to form a compound with a sandwich structure, the compound is directly precipitated in an external magnetic field, uncombined substances are removed through cleaning, and the detection sensitivity is high. And then re-dispersing the precipitate (compound) in the electrolyte, and immersing the glassy carbon electrode loaded by the graphene-modified nano porous membrane. After electrification, the cadmium ions in the compound are subjected to redox reaction to generate a current signal, and the current intensity of the current signal is in direct proportion to the content of alpha-synuclein. The sensor has the advantages of simplicity in operation, rapidness in detection, high sensitivity, high specificity and the like.

Described herein is a zwitterionic polymer-based coating that, when applied to an electrochemical biosensor, is capable of reducing fouling without compromising the current signal while also facilitating probe attachment.

The utility model discloses an electrochemical biosensor. The sensor comprises a base material, a first electrode and a second electrode. The base material is L-shaped, the first end of the base material is a test end, and the second end of the base material is a transmitting end. The at least one first electrode is arranged on the first surface of the base material, the first end of the first electrode is arranged on the transmitting end, the second end of the first electrode is arranged on the testing end, and the first end and the second end of the first electrode are connected through a first conductive connecting wire; the first end of the first electrode, the second end of the first electrode and the first conductive connecting line are located on the same plane. The at least one second electrode is arranged on the second surface of the base material, the first end of the second electrode is arranged on the transmitting end, the second end of the second electrode is arranged on the testing end, and the first end and the second end of the second electrode are connected through a second conductive connecting wire; the first end of the second electrode, the second end of the second electrode and the second conductive connecting line are located on the same plane. The base material is provided with a limiting structure, and through the design of 1-3 bayonets, the accuracy and firmness of the connection position of the sensor and other assemblies are ensured.

The present invention belongs to the field of electrochemical biosensor technology, and discloses a non-enzyme glucose sensor electrode based on CuCo bimetallic organic framework material and its preparation and application. The electrode includes a foam copper substrate and a multi-walled carbon nanotube material (MWCNTs), a nanogold material and a CuCo bimetallic organic framework material sequentially loaded on the surface of the foam copper substrate. Its preparation method includes the steps of: mixing an aqueous solution of a divalent copper salt and a divalent cobalt salt and a dimethylformamide solution of trimesic acid, performing a hydrothermal reaction to prepare a CuCo bimetallic organic framework material (CuCo‑BTC), sequentially coating a multi-walled carbon nanotube (MWCNT) dispersion, a nanogold dispersion and a CuCo bimetallic organic framework material (CuCo‑BTC) dispersion on a foam copper (CF) surface to obtain a non-enzyme glucose sensor electrode (CuCo‑BTC / Au / MWCNT / CF). The non-enzyme glucose sensor prepared by the present invention has a wide detection range, high sensitivity, and has good anti-interference performance for uric acid, dopamine, ascorbic acid and cysteine.

The invention provides a magnetic control type electrochemical biosensor of MWCNTs-MoS2 based on CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a as well as a preparation method and application of the magnetic control type electrochemical biosensor. The magnetic control type electrochemical biosensor comprises an aminated multi-walled carbon nanotube-molybdenum disulfide modified screen-printed carbon electrode, wherein a CRISPR-crRNA-Target DNA ternary complex and a ferroferric oxide-gold-single-stranded nucleotide probe are dropwise added on the surface of the screen-printed carbon electrode, and the probe is sheared after the reaction of the CRISPR-crRNA-Target DNA ternary complex and the ferroferric oxide-gold-single-stranded nucleotide probe; the magnetic control type electrochemical biosensor respectively shows wide linear response in the range of 4.6 * 10 < 1 >-4.6 * 10 < 7 > CFU / mL of staphylococcus aureus and 5.5 * 10 < 7 > CFU / mL of salmonella, the detection limit of the magnetic control type electrochemical biosensor to two pathogenic bacteria is as low as 2CFU / mL, and the magnetic control type electrochemical biosensor has good specificity to interfering bacteria, can be used for rapid detection of food safety and has good practicability.

A method for sensing an analyte utilizing a sensor having a working electrode, the method includes providing the working electrode with an analyte-specific enzyme and a redox mediator, providing the working electrode to the analyte, accumulating charge derived from the analyte reacting with the analyte-specific enzyme and the redox mediator for a set period of time, connecting the working electrode to circuit after the set period of time, and measuring the signal from the accumulated charge.