377 results about "Electrochemical biosensor" patented technology

A method for circumscribing an insulating barrier region around a singular conductive microneedle structure or plurality of conductive microneedle structures adhered to a fixed substrate for the purpose of spatially defining a conduit for the routing of an electrical signal from the surface of said microneedle or microneedles to the posterior surface of the substrate is disclosed herein. A microneedle-based electrochemical biosensors structure comprises a substrate, a microneedle biosensor, a primary electrically conductive element, a secondary electrically conductive element and an electrically insulative annular barrier.

The invention provides a preparation method of an electrochemical biosensor based on a PLL-coated VPAu modified nuclear track membrane, and belongs to the field of electrochemical biosensing analysis. The electrochemical biosensor for detecting troponin is constructed by adsorbing a PLL (at) VPAu composite nanomaterial synthesized by an ultrasonic liquid phase assisted method into a nuclear track membrane pore channel through electrostatic interaction and connecting a specific recognition molecule of a target to the surface of the pore channel modified by the PLL (at) VPAu by utilizing a bonding reaction mechanism. After a target object is captured by the recognition molecules connected to the nuclear track membrane, the effective aperture of the nuclear track membrane nanopores is reduced, and the electrochemical workstation can convert the biological behavior into an electric signal, so that high-sensitivity and rapid detection of the target protein is realized. The method has very important theoretical and practical significance in the field of medical diagnosis, and a new direction is provided for application of the nuclear track membrane in the field of analysis and detection.

A method for circumscribing an insulating barrier region around a singular conductive microneedle structure or plurality of conductive microneedle structures adhered to a fixed substrate for the purpose of spatially defining a conduit for the routing of an electrical signal from the surface of said microneedle or microneedles to the posterior surface of the substrate is disclosed herein. A microneedle-based electrochemical biosensors structure comprises a substrate, a microneedle biosensor, a primary electrically conductive element, a secondary electrically conductive element and an electrically insulative annular barrier.

The invention provides an integrated electrochemical biosensor capable of detecting double miRNA with high sensitivity, and the integrated electrochemical biosensor can simultaneously detect double miRNA markers with high sensitivity through self-assembly of a Y-shaped capture probe, preparation of an Au / TiO2-FTO array electrode, catalytic hairpin amplification (CHA) and construction of a micro-droplet chip electrochemical biosensor. The advantages of equalization of double-target miRNA capture probe sites, excellent electrode performance, reliable signal amplification, low sample consumption and the like are integrated, the linear range of miRNA21 and miRNA155 detection is 10 fM to 100 nM, and the detection limits are 5.00 fM and 5.17 fM respectively. By detecting the miRNA21 and miRNA155 double markers, the accuracy of breast cancer diagnosis is up to 98%, and the recognition sequences at the two ends of the Y stent probe are modified, so that the sensor can be used for diagnosing other miRNA marker related diseases. The integrated micro-droplet chip electrochemical biosensor constructed by the invention has a remarkable clinical diagnosis value and a prominent practical application prospect.

The invention provides an electrochemical biosensor based on a nanoparticle modified multilayer porous membrane and application of the electrochemical biosensor. The nano particle modified multilayer porous membrane is composed of a plurality of modified nano particles among membranes with nano pore channels. The electrochemical biosensor constructed based on the nanoparticle modified multilayer porous membrane has the following advantages: (1) good conductivity and stability; and (2) multiple capture probes are modified on the surfaces of the nano pore channels of the multi-layer porous membrane, so that multiple different targets can be respectively detected, and multiple different targets can also be detected at the same time. And (3) enzyme-free double-target sensitive normal-temperature amplification-free detection can be carried out. (4) preparation steps are simple, consumed time is short, and operability is high; and (5) the sensitivity and the specificity are high.

The invention belongs to the field of electrochemical biosensors, discloses a preparation method of an electrochemical biosensor capable of being used for multi-mode sweat detection, and particularly provides a single-electrode multifunctional arrangement method in a three-electrode system. A sensor material with high sensitivity and high selectivity is prepared, then the sensor material is printed through screen printing and assembled into a portable device to achieve simultaneous detection of multiple biomarkers in sweat, and the preparation method comprises the steps of selection and modification of an electrode material, sensor assembly and integration of a signal acquisition and analysis system. Through innovative material design and process optimization, the sensor not only can acquire information of various biomarkers in sweat in real time, but also can provide reliable data analysis, and shows excellent long-term stability and anti-interference capability in practical application. The technology provides an effective and reliable solution for real-time health monitoring.

The present invention relates to: an electrochemical biosensor capable of effectively and sensitively detecting alfuzosin; an electrode applied thereto; a metal-organic framework (MOF) which includes a zinc / cobalt oxide and is applied in manufacturing the electrode; and methods for manufacturing same.

The invention discloses a construction method and application of a zwitter-ion antifouling and dual-signal-ratio electrochemical biosensor, and belongs to the technical field of biochemical sensing analysis and life science. According to the invention, 3-aminopropyl dimethylamine oxide (APDMAO) zwitterions are prepared to be used as an antifouling interface. Specific positive and negative charges (N < + >-O-) of APDMAO promote hydrogen bond combination with water molecules, and a firm hydration barrier is formed. Meanwhile, the development of APDMAO overcomes the defect that zwitterions are difficult to synthesize and modify, and the application of APDMAO in biosensing analysis is expanded. A methylene blue (MB) internal standard modified alpha fetoprotein (AFP) aptamer is coupled with APDMAO under a controllable potential, so that the detection precision of the sensor is improved. The constructed ratio type electrochemical antifouling biosensor realizes sensitive and accurate detection of AFP, and the detection limit is as low as 3.41 fg / mL.

The invention discloses a paper-based electrode based on liquid metal assisted laser-induced graphene and a preparation method thereof.The paper-based electrode comprises a substrate layer, a functional layer, a conductive layer and a hydrophobic layer which are sequentially arranged, the substrate layer is made of paper, the functional layer is formed by coating liquid metal nanoparticles, and the conductive layer is formed by coating liquid metal nanoparticles. The conductive layer is an electrode formed by a laser-induced graphene technology, and the hydrophobic layer is a fluid control area formed by wax packaging. According to the invention, paper is used as a substrate, liquid metal is combined with a laser-induced graphene technology, surface modification is carried out through eutectic gallium indium (EGaIn), polyimide (PI) and N-methyl pyrrolidone (NMP) composite slurry, and then a conductive electrode pattern is formed on the surface of the paper substrate by using a laser-induced graphene (LIG) technology. The method has the advantages of simple preparation, low cost, environmental friendliness and the like, and the prepared paper-based electrochemical biosensor has excellent conductivity, mechanical flexibility and electrochemical activity.

The invention provides an electrochemical biosensor for detecting mycoplasma pneumoniae, the core of the electrochemical biosensor comprises a specific capture probe, and the nucleotide sequence of the specific capture probe is a complementary sequence of a 23S-5S rDNA spacer sequence (ITS) of the mycoplasma pneumoniae. In the preparation link, the porous membrane is used as a high-efficiency electron transmission channel, the process is remarkably simplified, and the reaction efficiency is improved. By virtue of a capture probe taking 23S-5S rDNA ITS as a target, the sensor is endowed with excellent specificity and high sensitivity, so that the sensor has excellent performance in complex biological sample detection, can accurately and efficiently recognize a target object and effectively avoids interference of other substances. The electrochemical biosensor is easy and convenient to operate and high in response speed, has wide application prospects in the fields of clinical diagnosis and rapid disease screening, can provide key technical support for timely diagnosis of mycoplasma pneumoniae infection, greatly meets the urgent requirements for rapid and accurate detection in clinical and practical application, and has good application prospects. The development process of a biological detection technology in the mycoplasma pneumoniae detection direction is powerfully promoted.

The invention provides a preparation method of a TiO2 / Au-C3N4 heterojunction photoelectrochemical biosensor and an application of the TiO2 / Au-C3N4 heterojunction photoelectrochemical biosensor. Comprising the following steps: step 1, preparing TiO2 / FTO; step 2, preparation of TiO2 / Au-C3N4 (titanium dioxide / Au-C3N4); step 3, preparing a nucleic acid aptamer; and step 4, using the confining liquid mercaptohexanol. The preparation method comprises the following steps: firstly, preparing a TiO2 thin film by an electrophoretic deposition method, and then combining the TiO2 thin film with graphite phase carbon nitride (g-C3N4) doped with high-conductivity noble metal gold nanoparticles (Au NPs) to form a heterojunction; under an illumination condition, Au NPs and photons interact to generate a regional surface plasma resonance phenomenon, and a strong electromagnetic field and high-concentration high-energy carriers are formed on the surface of the nano structure in the process, so that the photocurrent response of the composite material is remarkably enhanced; besides, the introduced Au NPs can also form an Au-S bond with the DNA aptamer containing the-SH group, and more binding sites are provided, so that the capture capability of the aptamer is enhanced.

The invention provides an electrochemical biosensor based on ultrasonic bidirectional driving and a detection method, and belongs to the technical field of biosensing. The sensor comprises a porous membrane and an ultrasonic bidirectional driving unit, and a probe capable of specifically capturing a target object is fixed in a pore channel of the porous membrane; the ultrasonic bidirectional driving unit is composed of a first ultrasonic transducer and a second ultrasonic transducer which are symmetrically arranged on the two sides of the porous membrane, and can synchronously execute reverse power gradient change to form alternately changing pressure difference on the two sides of the membrane so as to drive liquid to repeatedly penetrate through the porous membrane. The detection method comprises the steps of to-be-detected liquid injection and ultrasonic bidirectional driving, ultrasonic auxiliary cleaning and electrochemical detection. The problems of insufficient probe utilization rate, low mass transfer efficiency and non-specific adsorption of a traditional passive diffusion mechanism are solved by utilizing bidirectional ultrasonic active driving force, the detection sensitivity, speed and signal-to-noise ratio are remarkably improved, and the method is suitable for rapid and high-sensitivity quantitative analysis of various analytes.

A method for circumscribing an insulating barrier region around a singular conductive microneedle structure or plurality of conductive microneedle structures adhered to a fixed substrate for the purpose of spatially defining a conduit for the routing of an electrical signal from the surface of said microneedle or microneedles to the posterior surface of the substrate is disclosed herein. A microneedle-based electrochemical biosensors structure comprises a substrate, a microneedle biosensor, a primary electrically conductive element, a secondary electrically conductive element and an electrically insulative annular barrier.

The invention discloses a preparation method of an electrochemical biosensor based on neuraminidase, and relates to the field of electrochemical sensors. The method comprises the following steps: preparing N-Gr / MWCNTs, preparing N-Gr / MWCNTs (at) GCE, preparing Ppy / N-Gr / MWCNTs (at) GCE, and preparing NA / Ppy / N-Gr / MWCNTs (at) GCE. According to the electrochemical biosensor based on neuraminidase, MWCNTs and N-Gr are compounded to form an N-Gr / MWCNTs composite material, high-conductivity polypyrrole is polymerized on the N-Gr / MWCNTs composite material through pyrrole, the biosensor without a mediator is prepared, electrons can be directly transmitted between an electrode and a bioactive substance, and the electrochemical biosensor can be used for detecting neuraminidase. The response performance of the sensor is obviously improved; the electrochemical biosensor is based on the NA inhibition principle, is mainly used for rapidly screening antiviral components in traditional Chinese medicines, can realize rapid screening and evaluation of the antiviral components in the traditional Chinese medicines, belongs to a convenient, efficient and low-cost means, and also provides scientific basis and technical support for discovery and evaluation of active components.

The present invention relates to an electrode for an electrochemical biosensor for selectively detecting dopamine, an electrochemical biosensor comprising same, and a manufacturing method therefor and, specifically, to an electrode for an electrochemical biosensor for selectively detecting dopamine, an electrochemical biosensor comprising same, and a manufacturing method therefor, wherein the electrode has a lower trace detection limit compared with an existing dopamine detection sensor and exhibits superb stability, reproducibility, and selectivity.

The invention belongs to the technical field of electrochemical sensors, and particularly relates to a cobalt-based modified industrial solid waste vinegar residue derived carbon composite material and a preparation method and application thereof. The preparation method comprises the following steps: washing, drying, crushing and sieving industrial waste vinegar residues to obtain vinegar residue powder; carbonizing the vinegar residue powder in an inert atmosphere to obtain carbonized vinegar residues; the carbonized vinegar residues are mixed in a hot alkali solution for activating treatment, and then Co3O4 is derived through inorganic cobalt salt in-situ growth ZIF-67, so that the cobalt-based modified industrial solid waste vinegar residue derived carbon composite material is obtained. Porous activation is carried out on the surface of the carbonized vinegar residues through hot alkali, rich protruding wrinkles and pores are formed, the surface area is increased, rich nitrogen components and active carbon oxygen functional groups are reserved, and the catalytic reaction activity is improved. The material is used as a sensing material to prepare a high-performance electrochemical biosensor, and the constructed electrochemical sensor is used for detecting biomolecules such as hydrogen peroxide and the like to obtain a relatively wide detection range.

The invention discloses an electrochemical biosensing system for detecting BVDV (bovine viral diarrhea virus) based on CRISPR-Cas13a (clustered regularly interspaced short palindromic repeats). The electrochemical biosensing system comprises an electrochemical biosensor and a CRISPR-Cas13a system, the electrochemical biosensor comprises a working electrode, and the surface of the working electrode is modified with an MB-RNA probe; one end of the MB-RNA probe is modified with sulfydryl, the other end of the MB-RNA probe is modified with signal molecules, and the MB-RNA probe is connected with the working electrode through an Au-S bond; the CRISPR-Cas13a system comprises a crRNA (Complementary Ribonucleic Acid) group and Cas13a; and the crRNA group comprises at least one of BVDV (bovine viral diarrhea virus)-crRNA 2, BVDV-crRNA 7, BVDV-crRNA 8, BVDV-crRNA 11, BVDV-crRNA 12 and BVDV-crRNA 14. The electrochemical biosensing system disclosed by the invention has extremely high clinical sensitivity and clinical specificity on the BVDV virus.

A reagent formulation comprising: (a) an ester-containing substrate with an electrochemical mediator; (b) a buffer having a pH sufficiently low to preserve the substrate prior to introduction of a sample having a higher pH; (c) one or more chemical stabilizers; and (d) one or more surfactants.

The invention provides an MWCNTs-Au electrochemical biosensor based on RPA-CRISPR as well as a preparation method and application of the MWCNTs-Au electrochemical biosensor, and belongs to the technical field of preparation of electrochemical biosensors and detection of pathogenic bacteria of dairy products. The electrochemical biosensor disclosed by the invention comprises a multi-walled carbon nanotube-nanogold-hairpin nucleotide probe modified silk-screen printing carbon electrode and a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-crRNA-Target DNA (Deoxyribose Nucleic Acid) ternary complex, the CRISPR-crRNA-Target DNA ternary complex is assembled on the surface of the multi-walled carbon nanotube-nanogold-hairpin nucleotide probe modified silk-screen printing carbon electrode, and the CRISPR-crRNA-Target DNA ternary complex is assembled on the surface of the multi-walled carbon nanotube-nanogold-hairpin nucleotide probe The electrochemical biosensor shows wide linear response in a range of 1.04 * 10 < 1 > to 1.04 * 10 < 8 > CFU / mL, and the detection limit is as low as 3 CFU / mL; and the method has good practicability in detection of staphylococcus aureus.

The invention discloses an electrochemical biosensor of a light-driven nanopore channel and application of the electrochemical biosensor. According to the sensor, a photoresponse nanopore membrane is constructed, an outer surface aptamer probe is combined, asymmetric photothermal gradient driven ion transmission induced by near-infrared light is utilized, and ultra-sensitive detection on a target object is realized by regulating and controlling the charge density of the outer surface. According to the electrochemical biosensor provided by the invention, traditional voltage driving is replaced by asymmetric near-infrared light driving, so that the electrochemical biosensor has a relatively wide detection range and an extremely low detection limit, and high-sensitivity and specific detection on a target object can be realized. The electrochemical biosensor can be used for detecting microcystin-LR (MC-LR), the detection limit reaches 1 * 10 <-7 > mu g / L, the sensitivity is improved by 5 orders of magnitude compared with that of a traditional voltage-driven sensor, and the electrochemical biosensor can be applied to trace pollutant detection in the fields of environmental monitoring, food safety and the like.

The invention discloses an aptamer electrochemical biosensor based on an AuNPs / MXene nanometer material and a preparation method and application of the aptamer electrochemical biosensor. According to the electrochemical biosensor, AuNPs particles grow on the surface of MXene, an AuNPs / MXene composite material is prepared, then the composite material is fixed to the surface of an electrode through chitosan, aptamer probes P1 and P2 are hybridized with MCH, and then the aptamer probes P1 and P2 are self-assembled to the surface of the electrode through Au-S bonds. According to the invention, the AuNPs / MXene nano material is prepared, and the AuNPs / MXene nano material and the AuNPs / MXene nano material play a synergistic effect, so that the performance of the sensor is further improved, the sensor has a wide detection range and a low quantitative line, and rapid, sensitive and specific detection on a sample can be realized. The sensor can be used for in-situ detection of inflammatory factors at a wound, can be applied to non-invasive instant wound nursing monitoring equipment, and provides reliable and timely monitoring data to realize more effective diagnosis and treatment.

Electrochemical sensors based on metal cluster composites and methods of manufacture are provided. [Solution] Au 25 The graphene composite solution, the bean esterase chitosan solution, and the Nafion solution are dropped onto a glassy carbon electrode and then dried to obtain an electrochemical biosensor electrode. The metal cluster is composed of 25 gold atoms and a well-defined number of organic ligands, and has a precise composition and stable geometric structure. Au 25 The graphene composite has excellent electrical conductivity and cooperative electrocatalytic effect, which can effectively promote the transfer of electrons and improve the response sensitivity of the sensor. The electrochemical sensor can measure the content of profenofos by enzyme inhibition method using kidney bean esterase as the enzyme source.

The invention relates to the technical field of biosensing, in particular to enzyme liquid, an electrochemical biosensor for blood glucose measurement and a preparation method of the electrochemical biosensor. The enzyme liquid is used for preparing the electrochemical biosensor for blood glucose determination and comprises the following components in percentage by mass: 60%-80% of a buffer system, 10%-20% of a disintegrating agent, 0.1%-5% of a stabilizer, 0.01%-2% of a surfactant, 0.1%-8% of an electron transfer agent and 0.1%-5% of glucose dehydrogenase, the electron transfer agent is selected from a phenanthrenequinone compound or a phenanthroline compound containing diketone. The current amplification effect of the electrochemical biosensor can be improved, and the detection sensitivity can be further improved.

The invention relates to the technical field of electrochemical analysis and detection, in particular to an electrochemical biosensor based on CRISPR / Cas12a as well as a preparation method, a detection method and application of the electrochemical biosensor. An electrochemical biosensor based on CRISPR / Cas12a comprises a Cas12a-crRNA detection system, an APT-ActDNA adaptation system and an electrochemical aptamer sensor, and when a target analyte exists, the Cas12a-crRNA detection system and the APT-ActDNA adaptation system are matched with each other to react with the target analyte, so that the electrochemical aptamer sensor generates an electrochemical signal. According to the technical scheme, the problems of specific recognition and detection of amyloid beta peptide (Abeta) in a complex biological sample are solved, the preparation method is simple, the cost is low, and the detection method is high in sensitivity and accuracy.

The invention discloses ultrasensitive detection of vibrio parahaemolyticus by a CRISPR (clustered regularly interspaced short palindromic repeats) electrochemical biosensor based on PCR (polymerase chain reaction) and a hairpin DNA (deoxyribonucleic acid) probe, which comprises the following steps: preparing a gold nanoelectrode, modifying the gold nanoelectrode by using the hairpin DNA probe, performing polymerase chain reaction amplification to obtain a PCR amplification product, adding the PCR amplification product into a CRISPR system, uniformly oscillating, and detecting the vibrio parahaemolyticus by using the CRISPR electrochemical biosensor. Placing in a 37 DEG C constant-temperature metal bath for pre-reaction for 10 minutes, and dropwise adding the obtained reaction system to the surface of the hairpin DNA probe modified gold nanoelectrode. The method disclosed by the invention is high in overall repeatability and stability, the modification density of the surface of the electrode can be reduced, the steric hindrance effect is further reduced, the trans-cutting efficiency is improved, and in actual detection, the method shows remarkable sensitivity, is high in specificity, short in time consumption, low in cost and simple and convenient to operate, and can be applied to field detection.

The invention discloses an electrochemical biosensor for detecting mycobacterium tuberculosis. The sensor takes a porous membrane as a base material, the inner wall of a pore channel of the porous membrane is co-modified with a polydopamine (PDA) and conductive carbon material composite layer, and an amino-modified specific capture probe based on a mycobacterium tuberculosis ESAT-6 gene is fixed on a modification layer through covalent binding. When a target gene is captured by a probe, the volume effect of the target gene causes blockage of a porous channel of the porous membrane, so that electrochemical signals (such as current) of the sensor are changed, and high-sensitivity and specific detection of mycobacterium tuberculosis is realized. According to the invention, a porous structure, biological probe specific fixation and an electrochemical signal conversion technology are combined, and a novel efficient tool is provided for rapid diagnosis of tuberculosis.

Electrochemical biosensors are devices that convert biological signals into electrical signals for detection. Described herein are automation-compatible high-throughput electrochemical sensing devices and systems that form a high-throughput electrochemical sensing platform. In certain embodiments, the platform includes: a sensor plate assembly with a sensor board and multi-well plate, where each well contains at least one three- electrode assembly (e.g., a working electrode, a reference electrode, and a counter electrode) with a conductive antifouling coating; a control board for detecting signals from each of the multiple electrode assemblies; an optional heater and / or temperature controller for the sensor board; and an interconnect assembly to facilitate replacement of the sensor board.

The invention provides construction of an electrochemical biosensor based on a fucoidin hydrogel microneedle and application of the electrochemical biosensor in online monitoring of uric acid, and relates to the technical field of biosensors. According to the micro-needle, Gantrez S-97 and PEG are subjected to copolymerization crosslinking to form a hydrogel main body, fucoidin is introduced for blending modification, a three-dimensional network structure is constructed by utilizing a high-temperature esterification reaction for regulating and controlling pH, and the super-expansion hydrogel micro-needle with high swelling property and good biocompatibility is formed. The microneedle can rapidly collect a skin interstitial fluid sample, and then through a screen printing electrode (SPE) platform, carboxylated MWCNTs are used for modifying an electrode and immobilizing uricase to construct an enzyme sensor. Hydrogen peroxide generated by catalyzing uric acid on the surface of the electrode participates in electrochemical reaction, and an output signal is used for real-time detection of uric acid concentration. The device integrates minimally invasive sampling and high-sensitivity electrochemical detection, has the advantages of quick response, simplicity and convenience in operation, good biocompatibility and the like, and is suitable for application scenes of continuous monitoring of uric acid, chronic disease management and the like.

The invention designs a preparation method of an electrochemical sensor for detecting acrylamide based on a Ce-MOF and AuPd nanoflower composite material. The advantages of large surface area and good stability of Ce-MOF are utilized to load a large amount of AuPd NFs, and the composite material Ce-MOF (at) AuPd NFs with high conductivity is obtained. The preparation method comprises the following steps: modifying an electrode by virtue of a physical adsorption effect, fixing a triple-helix molecular switch THMS on the electrode by virtue of a gold-sulfur bond and a palladium-sulfur bond, when acrylamide exists, combining acrylamide with an aptamer, forming DNAzyme by virtue of single-stranded CDNA1 and single-stranded CDNA2, and driving a DNA walker amplification strategy in the presence of Mg < 2 + >, so that a hairpin HP1 is sheared, and a large amount of single-stranded S1 is obtained. According to the method, S1, signal tags combined on an electrode are reduced and signals are reduced by destroying a triple helix structure, so that acrylamide detection is realized. The electrochemical biosensor constructed by the method disclosed by the invention has relatively high specificity and relatively high sensitivity.

The invention discloses a preparation method of an enzyme biosensor for simultaneously detecting multiple mycotoxins, the sensor and application. According to the method, the carbon nano tube-nano gold laccase electrochemical biosensor is prepared through a layer-by-layer dispensing method, the preparation process is simple and rapid, operation is easy, the cost is low, a carbon nano tube modified electrode has reusability, the catalytic activity and stability of an enzyme modified by a nano material are remarkably improved, and the electrochemical biosensor has good application prospects. The obtained biosensor can perform high-flux simultaneous detection on mycotoxins such as aflatoxin B1, zearalenone and the like, is high in detection sensitivity, good in selectivity and small in reagent consumption, has the detection limit of the aflatoxin B1 of 0.012 mu mol / L and the detection range of 0.05-100 mu mol / L, has the detection limit of the zearalenone of 0.007 mu mol / L and the detection range of 0.01-120 mu mol / L, has the low detection limit and the wide detection range, and can be widely applied to detection of mycotoxins such as aflatoxin B1, zearalenone and the like. The problems of low flux, complex electrode preparation and poor stability and sensitivity of a traditional electrochemical detection mode are solved, and the method can be widely applied to rapid detection under the condition of co-pollution of multiple fungimycin in cereals.