1109 results about "Detection limit" patented technology

The invention provides an online quality detection method of a nerve-soothing and brain-nourishing liquid based on multispectral fusion, and aims at solving the bottleneck problems of long offline time consumption, weak multi-index detection capability, poor dynamic adaptability and the like in the traditional detection technology, and realizing intelligent quality control of the whole process of the production technology. The device integrates an ultraviolet-visible spectrum module, a near-infrared spectrum module and a fluorescence spectrum module, constructs a multi-scale residual convolutional network to extract near-infrared spectrum characteristics, analyzes a fluorescence excitation-emission matrix through bidirectional LSTM, and cooperates with dynamic baseline correction of an ultraviolet spectrum, so that the icariin content detection precision is improved by 40% compared with that of a traditional HPLC method, the heavy metal residue detection lower limit reaches 0.05 ppm, and the detection accuracy is greatly improved. And an innovative solution is provided for intelligent production of traditional Chinese medicine preparations.

The invention discloses a high-sensitivity carbendazim detection method based on terahertz metamaterial resonance enhancement, and provides a terahertz metamaterial sensor with a cross-shaped composite double-peak structure, a terahertz spectrum technology is utilized to collect transmission spectrums, the difference of different concentrations among terahertz spectrums is explored, and the detection accuracy is improved. The analysis finds that the transmission peak amplitude of the terahertz transmission spectrum of the structure is in a decreasing trend along with the increase of the concentration of a carbendazim solution, data is preprocessed through multiple scatter correction (MSC), feature extraction is performed by adopting an iterative information variable reservation (IRIV) method, and a quantitative detection model of the carbendazim terahertz transmission spectrum is established based on an LS-SVM (least squares support vector machine). The invention provides a novel approach for quantitative detection of carbendazim, and experimental results show that the RP of the model reaches 0.9825, the RMSEP is 0.2001, and the detection limit LOD is 0.672 mu g / ml. The invention provides a new method for rapid, lossless and high-sensitivity detection of carbendazim in food.

The invention relates to the technical field of analysis and detection, in particular to a micro-area distribution detection method for micro and / or trace component elements in nickel-based single crystal superalloy. According to the method, the conventional common element distribution detection means (EPMA, EDS, TOF-SIMS, TEM and EELS) are combined, the detection limit and the detection advantages are referred, different devices are reasonably utilized by analyzing different phase structure characteristics, quantitative or semi-quantitative element micro-area distribution analysis is quickly and effectively realized, the use requirements on high-precision devices are reduced, and the method is suitable for large-scale popularization and application. And the cost and the test difficulty are greatly reduced.

The invention belongs to the technical field of monatomic nano-enzyme material preparation, and particularly relates to a nano-enzyme preparation method for acetylcholin esterase activity detection. The Ni-NiO heterojunction material is successfully synthesized by adopting Ni-MOF as a carrier and accurately regulating and controlling the ratio of nitrogen to air in the ultrafast Joule hot calcination process. The material has rich oxygen vacancies and metal Ni sites, the oxygen vacancies and the metal Ni sites can effectively serve as anchoring sites of Pt single atoms, the loading capacity and dispersity of the Pt single atoms are remarkably improved, and therefore the excellent nano-enzyme catalytic activity is shown. In addition, the preparation method disclosed by the invention is simple and convenient in process and has a wide industrial application prospect. An acetylcholin esterase activity colorimetric sensing method constructed on the basis of the nanometer enzyme for acetylcholin esterase activity detection prepared by the method has extremely high sensitivity and low detection limit. The detection limit of the technology can be as low as 0.037 mU / mL, the problem that the sensitivity of a traditional detection method in a low-concentration sample is insufficient is remarkably solved, and remarkable clinical application potential is shown.

The invention discloses a near-infrared fluorescent probe for detecting ONOO <-> as well as a preparation method and application of the near-infrared fluorescent probe, and belongs to the technical field of peroxynitroso ion detection. The near-infrared fluorescent probe for detecting ONOO <-> provided by the invention is high in detection speed, and the highest fluorescence intensity can be reached within 60 seconds; the reaction is obvious and easy to observe, when the compound reacts with ONOO <->, strong red fluorescence can be generated, and the change of the color of the solution can be observed by naked eyes; the selectivity is strong, the sensitivity is high, the interference of other biological molecules and active substances can be effectively avoided, the accuracy and the reliability of a detection result are ensured, and the lowest detection limit can reach 107nM; the hydrogel has good biocompatibility, is low in toxicity in a living body, and does not generate adverse effects on normal physiological functions of a biological sample; the provided preparation method is simple and easy to operate, the raw materials are low in cost and easy to obtain, the synthesis efficiency is high, and the preparation method can be used for large-scale industrial production and has wide application prospects.

The invention discloses a specific primer for distinguishing rice and barnyard grass and application of the specific primer, and belongs to the technical field of biological species identification. The sequences of the specific primers are as shown in SEQ ID NO. 1 and SEQ ID NO. 2. The specific primer is designed according to the nuclear gene ITS sequence of the rice, the specific primer can accurately identify the rice through PCR, the identification method is stable, the detection time is short, the specificity is high, the detection limit is low, the identification method is not affected by material characters, rapid and accurate identification of the rice and barnyard grass can be achieved, and the identification efficiency is high. The problem that a method for effectively and rapidly distinguishing rice and barnyard grass does not exist at present is solved.

The invention discloses a method for accurately deducting Sr double-charge interference in in-situ Ca isotope determination, and relates to the technical field of isotope analysis. According to the method, the Sr double-charge interference in the in-situ Ca isotope measurement is accurately deducted through an iteration method, the Ca isotope measurement result can be simply and quickly corrected, and high-resolution, low-detection-limit and high-flux Ca isotope measurement is realized; ca single-charge fractionation factors are calculated through an iterative method, and instrument quality discrimination correction and accurate deduction of Sr double-charge interference are simultaneously realized through formulas 1-5 in combination with the Sr isotope ratio; the method breaks through the application limitation of in-situ Ca isotope measurement, is suitable for high-Sr and low-Sr samples, solves the technical problems of in-situ Ca isotope analysis accuracy and analysis precision, fills the technical blank of Ca isotope measurement in the Sr-rich sample in laser in-situ detection, and provides a firm technical support for application in the field of geoscience.

The present invention relates to the technical field of test and detection, and particularly to an on-line measurement method and device for total iron in power station water vapor. A catalytic reagent is added to the water sample to be measured and acts through an ultraviolet light oxidizer, so as to completely convert the colloidal iron in the water sample into ferric ions. The dissolution and conversion of the colloidal iron are completed without adding acid throughout the process. Since no acid is added during the process of converting the whole colloidal iron into dissolved iron, only a chromogenic reagent needs to be added to accurately measure the total iron content in the water sample, and it is also easier to realize the on-line measurement of total iron in the water sample. When the total iron content in the water sample is less than 10 μg / L, the water sample enters the concentration system for concentration and then optical measurement, so that the low-concentration total iron in the water sample can also be accurately measured. This measurement method can automatically measure the total content in the water sample on-line, avoid manual sampling and detection, can monitor the total iron in the water sample in real time, and has a lower detection limit and more accurate detection results, which is beneficial to the adjustment of the water condition during the start-up and shutdown stages of the power station.

The invention discloses a staphylococcus aureus biosensor based on a nucleic acid aptamer and a preparation method of the staphylococcus aureus biosensor. Three fluorine atoms (F) are introduced into the 5'end, the 3 'end and the middle position of an aptamer 2' F3-(5 '3'm DNA) sequence (SEQ ID NO: 1) for modification, so that the binding force of the aptamer and graphene is remarkably enhanced, the signal stability and the anti-interference capability of the biosensor are remarkably improved, non-specific fluorescence leakage can be effectively blocked, and reliable guarantee is provided for complex sample detection. According to the present invention, with the cooperation of the graphene oxide quenching substrate, the high-sensitivity detection of the staphylococcus aureus is achieved, the detection limit of the sensor within 5 min can achieve 5 cells / mL, the sensitivity is high, the specificity on the complex sample is more than 95.4%, and the sensor is suitable for the food safety rapid screening and the clinical diagnosis.

The invention relates to an extracellular vesicle surface protein detection paper-based kit and application thereof, and belongs to the technical field of biology. The technical problems that an existing sEVs detection technology is insufficient in specificity, depends on professional instruments and cannot meet the instant detection requirement are solved. According to the method, sEVs are synergistically captured through double aptamers, the target specificity is improved, signals are amplified in a cascaded mode through hyper-branched rolling circle amplification (HRCA) and a CRISPR / Cas12a system, and visual detection is achieved by detecting FAM-biotin probe separation before and after cutting through a transverse flow test strip. According to the method, interference of free protein is effectively avoided through double-target recognition, and the detection limit is as low as 406 parts / mL; cRISPR and a paper-based technology are combined to realize high sensitivity and instant detection; the system is simple and convenient to operate, does not need professional instruments, is suitable for tumor diagnosis and treatment monitoring of clinical samples, and provides a portable solution for precise medical treatment.

The invention relates to the technical field of pesticide detection, and discloses a pesticide residue detection system for agricultural products, comprising: a high-precision detection module comprising one or more detection units adopting a high performance liquid chromatography-mass spectrometry (HPLC-MS / MS) technology, each detection unit being configured with a detection limit of not less than 1 ng / g, the resolution ratio is not lower than 0.01 ppm, and the quantitative analysis precision is high. And the intelligent data analysis software integrates a pesticide residue data analysis engine based on an artificial intelligence (AI) algorithm, and quickly and accurately judges the types and residual quantity of pesticides. And the standardized data interface and sharing platform is designed with a unified data exchange format and an interface standard. By adopting a high-precision detection module and combining a high performance liquid chromatography-mass spectrometry (HPLC-MS / MS) technology, the system realizes multi-channel parallel processing and high-sensitivity detection, ensures the accuracy of a detection result, effectively solves the problem that the detection technology and the equipment level are uneven, and improves the overall detection level.

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

The invention belongs to the field of fluorescence detection, and particularly relates to a ratio-type fluorescent nanoprobe for specifically detecting tetracycline antibiotics as well as a preparation method and an application method of the ratio-type fluorescent nanoprobe. The nano-probe is a europium modified zinc gallium germanate nano-material, and is prepared by amino modification, citric acid modification and europium modification of the zinc gallium germanate nano-material. The probe can be specifically combined with tetracycline antibiotics; the fluorescent probe has two different fluorescence emission intensities, so that interference can be effectively eliminated, and the detection accuracy is high. And the synthesis method is simple and low in cost. The ratio type fluorescent nanoprobe provided by the invention also can realize the change of different colors under an ultraviolet lamp, can realize quantitative visual detection of tetracycline by combining the RGB value and the tetracycline concentration change, and can realize rapid on-site detection of tetracycline in a sample without the help of a fluorescent instrument. The tetracycline fluorescent probe is stable in optical performance, not prone to photobleaching, high in tetracycline recognition capacity, wide in response range (0.2-70 [mu] M) and low in detection limit, and has remarkable industrial application potential.

The invention belongs to the field of biomedical treatment, and particularly discloses a series of cancer cell early detection probes, a kit and a detection method. Aiming at the problems of low sensitivity, poor specificity, complex operation, long detection period and the like of the existing cancer cell detection means, various innovative probes are invented. Ten probes such as a quantum dot FRET probe based on a nucleic acid aptamer and a gold nano-cluster-antibody composite probe have different characteristics, and accurate recognition and detection of cancer cell markers are achieved through unique design principles such as aptamer screening, nano-material modification and a signal amplification strategy. Through synergistic comparison, the sensitivity of the probes is greatly improved, the lower detection limit is as low as 0.3-1.5 pg / mL and is improved by 30%-60% compared with that of a traditional method, the specificity and accuracy are high, the detection time is shortened to 1-2 hours, the sample demand is low, and the probes also have diagnosis and treatment integration and multi-mode imaging functions, and provide powerful support for early diagnosis and treatment of cancers.

The invention belongs to the technical field of biosensors, and provides a tetracycline ultra-sensitive detection method based on a CdTe (at) PVP (at) ZIF-8 core-shell material and a cascade amplification sensor. The sensor comprises an Apt-T composite probe, an M-2P chain, a complementary chain C, a self-locking DNAzyme hairpin chain and a hairpin chain H, the Apt-T composite probe is obtained by hybridizing an aptamer Apt and a T chain; the nucleotide sequences of the Apt, the T chain, the template chain M, the enriched chain P, the complementary chain C, the DNAzyme chain and the H chain are as shown in SEQ ID NO: 1-7. The sensor disclosed by the invention has the advantages of broad-spectrum recognition, high specificity, high detection speed, simplicity in operation, low detection limit and the like, can make up for the defects and deficiencies of an existing detection method for tetracycline antibiotics, realizes rapid and accurate ultra-sensitive detection on the tetracycline antibiotics, and has potential application prospects in food safety and environment detection.

The invention relates to an EuW10 / ZIF-8 composite material, a lead ion (Pb < 2 + >) detection and adsorption composite membrane based on the EuW10 / ZIF-8 composite material and a preparation method of the composite membrane, the composite membrane material can rapidly and sensitively detect Pb < 2 + > in a water body and efficiently remove Pb < 2 + > in wastewater, and meanwhile, the composite membrane material has good recycling performance. The EuW10 / ZIF-8 / PVA / PIL composite membrane disclosed by the invention has high-sensitivity detection capability on Pb, effective detection can be realized in a range of 1-106 mu M, and the detection limit is as low as 1 mu M. Other common heavy metal ions have extremely small interference on detection, the selectivity is strong, the concentration of Pb < 2 + > in water can be accurately detected, and a reliable basis is provided for water quality monitoring.

The invention provides an electronic probe quantitative analysis method for trace fluorine elements in an iron-containing sample. According to the quantitative analysis method, during quantitative analysis, the TAPL light splitting crystal is used, the TAPL light splitting crystal has a larger diffraction area, and the obtained count is higher; the interference of the light splitting crystal Fe of the LDE1L is difficult to process; therefore, the TAPL light splitting crystal is used for replacing the LDE1L light splitting crystal and the TAP light splitting crystal; the quantitative analysis conditions are as follows: the acceleration voltage is 15 kV, the beam current is 300 nA, and the beam spot is 20 microns; a TAPL light splitting crystal is adopted, the F content detection limit is 76 ppm (3 sigma), and a TAP light splitting crystal is adopted, the F content detection limit is 195 ppm (3 sigma); when the TAPL spectroscopic crystal is used for testing the trace fluorine element in the iron-containing sample, the detection limit is lower, the detection value is closer to the reference value, and the accuracy is higher.

The invention belongs to the field of analysis and detection, and provides a non-diagnostic method for detecting uric acid in serum based on biochar magnetic nano-enzyme colorimetry. According to the method, the biochar magnetic nano-enzyme is used for activating the potassium hydrogen peroxymonosulfate, then the 3, 3 ', 5, 5'-tetramethylbenzidine is oxidized to generate a blue characteristic product ox-TMB, and the uric acid can inhibit the reaction process, that is, within a certain quantity range, the concentration of the uric acid can be judged according to the change of the absorbance of a reaction system. The nano enzyme is easy to synthesize, low in cost and capable of being stored for a long time, and the uric acid detection method constructed by the invention is low in detection limit (3.85 mu mol / L), wide in linear range (50-1000 mu mol / L), relatively simple, rapid and cheap, and expected to be widely applied to serum uric acid detection.

The invention discloses a detection system and method for measuring trace Cr in tobacco, and relates to the technical field of tobacco detection.The system comprises a multi-stage dynamic digestion module, a two-channel solid-phase microextraction module, a high-resolution ICP-MS detection module, an intelligent data processing module, a collision reaction tank (CRC) based on the high-resolution ICP-MS module and a dynamic background correction algorithm. The detection limit of Cr is as low as 0.008 [mu] g / kg by combining the enrichment capacity of 50 times or more of two-channel solid-phase microextraction (DSPME), the detection limit is reduced by one order of magnitude compared with that of a traditional atomic absorption method (0.1 [mu] g / kg), the accurate determination requirement of trace Cr is met, the two-channel DSPME module is designed through physical isolation of amino magnetic nanoparticles (Cr (III) adsorption) and DTC mesoporous carbon (Cr (VI) adsorption), and the detection limit of the trace Cr is reduced by one order of magnitude compared with that of the traditional atomic absorption method (0.1 [mu] g / kg). And independent capture and elution of Cr (III) and Cr (VI) are realized.

The invention belongs to the technical field of fluorescent sensing materials, and particularly relates to a ratio metering type fluorescent probe based on a metal organic framework (MOF) material as well as a preparation method and application of the ratio metering type fluorescent probe. The probe material emits blue light, after 2, 6-dipicolinic acid (DPA) is added, the DPA enhances fluorescence emission of Tb < 3 + > and inhibits emission of coumarin through an antenna effect, the fluorescence intensity ratio (I548 / I429) between two emission bands is changed, a good linear response relation is shown in the concentration range of 0-90.0 mu M, the detection limit is 0.13 mu M, and high-sensitivity ratio type detection of the DPA is achieved. After detection is finished, phosphate radical (PO4 < 3->) ions are added, so that the antenna effect can be effectively quenched, the initial fluorescence state is recovered, and meanwhile, the method can be used for detecting the concentration of phosphate radicals. After DPA is added again, reactivation can be realized, so that recycling can be realized, and the limitation of disposable use of a traditional fluorescent probe is broken through.

The invention relates to the technical field of pharmaceutical analysis, and particularly discloses a method for detecting related substances of 4-chlorobenzoyl chloride. According to the method, a chromatographic column with 50% of phenyl and 50% of methylpolysiloxane as a stationary liquid is adopted to perform gas chromatographic analysis on a 4-chlorobenzoyl chloride sample, and accurate detection of four related substances, namely methyl parachlorobenzoate, ethyl parachlorobenzoate, o-chlorobenzoyl chloride and m-chlorobenzoyl chloride, in the 4-chlorobenzoyl chloride and the content of the four related substances is realized at the same time. The detection method is high in specificity, low in detection limit and quantitation limit, good in linear relation, high in recovery rate, good in repeatability, good in stability, good in durability, easy and rapid to operate, low in detection cost and accurate and reliable in detection result, and guarantees are provided for monitoring the quality stability and clinical medication safety of rebamipide.

The invention discloses an electrochemical biosensor of a light-driven nanopore channel and application of the electrochemical biosensor. According to the sensor, a photoresponse nanopore membrane is constructed, an outer surface aptamer probe is combined, asymmetric photothermal gradient driven ion transmission induced by near-infrared light is utilized, and ultra-sensitive detection on a target object is realized by regulating and controlling the charge density of the outer surface. According to the electrochemical biosensor provided by the invention, traditional voltage driving is replaced by asymmetric near-infrared light driving, so that the electrochemical biosensor has a relatively wide detection range and an extremely low detection limit, and high-sensitivity and specific detection on a target object can be realized. The electrochemical biosensor can be used for detecting microcystin-LR (MC-LR), the detection limit reaches 1 * 10 <-7 > mu g / L, the sensitivity is improved by 5 orders of magnitude compared with that of a traditional voltage-driven sensor, and the electrochemical biosensor can be applied to trace pollutant detection in the fields of environmental monitoring, food safety and the like.

The invention provides a cyanide generation and absorption efficiency monitoring method based on double-color indicator gradient change, and belongs to the technical field of cyanide monitoring, the absorption process is visually reflected through the double-color indicator gradient change, the absorption efficiency can be rapidly judged through color change without a complex instrument, the operation is simple and convenient, and the cost is low. And gradient color development and spectral analysis are combined, so that the detection limit can reach a mu g / L level, the trace detection requirement is met, and the absorption condition of the low-concentration cyanide can be accurately monitored. Through real-time change of the color gradient, the concentration distribution and the reaction process of the cyanide in the absorption process can be dynamically reflected, real-time data support is provided for industrial process control and environmental emergency monitoring, expensive detection equipment is not needed, the reagent cost is low, and the method is suitable for large-scale popularization and application.

The invention discloses a preparation method and application of an HPV16 / 18 type E6 / E7 protein multi-target detection test strip, and belongs to the technical field of biological immunodetection analysis. The detection test strip comprises a back plate, a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad, AIE fluorescent microsphere labeled probes are distributed on the combination pad, and a capture antibody and an AIE solution are distributed on the nitrocellulose membrane. The preparation method comprises the following three steps: preparation of the nitrocellulose membrane, preparation of the combination pad and assembly of the test strip. The detection test strip can synchronously and quantitatively detect PV16 E6, HPV16 E7, HPV18 E6 and HPV18 E7, each target corresponds to four signal points, multi-target synchronous rapid detection is achieved, the linear range is wide, the detection limit is low, operation is easy and convenient, cost is low, and the detection test strip is expected to become a novel efficient and reliable on-site screening tool for cervical cancer and precancerous lesions.

The invention discloses a hapten and an artificial antigen for detecting chlorfenapyr and application of the hapten and the artificial antigen. A time-resolved fluorescent test strip for detecting chlorfenapyr is prepared from the artificial antigen. The kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like, the quantitative detection limit of chlorfenapyr is as low as 3.48 ng / mL, the addition recovery rate is 99.6%-110.4%, the coefficient of variation is 2.1%-10.9%, and the kit is very suitable for rapid qualitative or quantitative detection of chlorfenapyr in plant-derived food by basic field personnel.

The invention discloses a method for measuring the content of impurity elements in high-purity quartz by ICP-MS (Inductively Coupled Plasma Mass Spectrometry). The method is characterized by comprising the following steps: S1, drying a sample; s2, weighing a sample; s3, primary dissolution with strong acid I; s4, secondary dissolution with strong acid II; s5, cooling and constant volume; and S6, respectively testing and analyzing the standard series working solution, the sample solution and the blank solution on ICP-MS (Inductively Coupled Plasma Mass Spectrometry). According to the method, the influence of impurity elements brought in in the sample treatment process on the determination result is reduced, the harm of precipitation generated by some metal ions and chloride ions on the accuracy of the result is avoided, the inductively coupled plasma mass spectrometer is adopted for analysis in the later period, the detection limit is greatly reduced, and the accuracy of the result is improved. Therefore, the purposes of reducing the lower limit of impurity element content measurement and increasing the upper limit of the silicon dioxide content range (the applicable silicon dioxide content range of the method can be higher than 99.99%) are achieved.

The invention relates to the technical field of biological detection, in particular to an MWCNTs-COOH / AuNPs / CS / Apt electrode modification material, an electrode and a preparation method and application of the MWCNTs-COOH / AuNPs / CS / Apt electrode modification material. A glassy carbon electrode is used as a substrate, and a carboxylated multi-walled carbon nanotube / gold nanoparticle / chitosan (MWCNTs-COOH / AuNPs / CS) nano composite material layer, an aptamer (Apt) recognition layer and a mercaptohexanol (MCH) closing layer are sequentially modified. The preparation method comprises the following steps: hydrothermally synthesizing AuNPs, preparing an MWCNTs-COOH / AuNPs / CS compound through ultrasonic dispersion, modifying the surface of an electrode layer by layer and the like. The aptamer immobilization technology and detection conditions are optimized, the sensor has high-sensitivity linear response to 8-OHdG within the range of 0.0706-7.06 mu M, the detection limit reaches 0.33 mu M, and the anti-interference performance is high (interference signal lt; 5%). The method solves the problems that a traditional detection method is high in cost and long in consumed time and an existing sensor is insufficient in sensitivity, and is suitable for rapid and accurate analysis of 8-OHdG in biomedical detection and environmental monitoring.

The present invention discloses the application of a water-soluble and positively charged fluorescent probe TPA-3Py for the detection of sodium heparin. TPA-3Py is obtained by covalently connecting three methylated vinylpyridines to the three arms of a triphenylamine molecule respectively. Heparin can induce the aggregation-induced emission effect of this fluorescent molecule. With the increase of the heparin content, the fluorescence intensity increases significantly, showing a good fluorescence turn-on response, overcoming the deficiency that the fluorescence probe quenches due to aggregation during the detection of heparin in the prior art. When a 10 μM aqueous solution of TPA-3Py is used for the detection of heparin, a stable fluorescence intensity is achieved within 5 minutes, the linear range is 0 - 6.14 mg / L (R<supgt;2< / supgt> = 0.9938), the lowest detection limit is 49.53 ng / mL, and the quantum yield reaches 4.85%. For the detection of sodium heparin, the TPA-3Py probe adopted in the present invention overcomes the quenching phenomenon caused by aggregation and exhibits a significant AIE effect. The fluorescent probe TPA-3Py for the detection of sodium heparin in the present invention has high sensitivity, high fluorescence intensity, rapid response and stable signal. The fluorescent molecular structure of TPA-3Py is simple, and the detection method is simple and easy to implement, which is worthy of popularization and application in the determination of the sodium heparin content.

The present invention discloses a covalent organic polymer fluorescent probe, and the preparation method is as follows: 2,7-dibromopyrene and 1,2,3,4,5,6-hexa(4-bromophenyl)benzene are added to an organic solvent to dissolve, a Ni(0) catalyst is added, and the reaction is heated overnight. After the reaction is completed, it is cooled to room temperature, an HCl aqueous solution is added, the resulting precipitate is filtered, and washed with an organic solvent to finally obtain the covalent organic polymer fluorescent probe. The fluorescent probe is synthesized by a nickel-catalyzed Yamamoto reaction, has a significant quenching effect on nitro explosives containing OH groups, can be observed with the naked eye to have a significant color change, has high sensitivity, and has a low detection limit; and has no significant response to nitro explosives containing OH groups. Therefore, this fluorescent probe can be used as a specific fluorescent probe for highly selective detection of nitro explosives containing OH groups.

The invention belongs to the technical field of non-metallic material nondestructive testing, and particularly relates to a method for evaluating the defect detection capability of a coplanar capacitive sensor. According to the defect detection capability evaluation method, defects under different buried depths and sizes are scanned, the distortion rate of a detection signal is calculated, and a preset threshold value is given to judge whether the defects are detected or not; and then a visual chart is drawn, and evaluation of the defect detection limit capability of the coplanar capacitive sensor is realized. The invention discloses a coplanar capacitive sensor defect detection capability evaluation method. The method comprises the following steps: determining a positive sensitivity region, a negative sensitivity region and a zero sensitivity region of a coplanar capacitive sensor; constructing to obtain a non-metallic material defect test group; establishing an electrostatic field simulation model; solving a distortion rate D of the detection signal; drawing a relation curve of detection signal distortion rates D under different burial depth positions and different defect sizes; giving a preset threshold value A, and recording a comparison result; and drawing a comparison result in a visual chart.