General rapid detection method for micromolecular poisonous and harmful substances in liquid milk

A technology for harmful substances and detection methods, which is applied in the field of detection of small molecules of toxic and harmful substances in liquid milk, can solve the problems of incompatible compounds, difficulty in improving monitoring efficiency, lack of versatility, etc. Risky, versatile effect

Inactive Publication Date: 2012-04-18
NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
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  • Abstract
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  • Application Information

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Problems solved by technology

The reason is that the pretreatment and instrumental analysis processes of these methods are difficult to be compatible with compounds of different...

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  • General rapid detection method for micromolecular poisonous and harmful substances in liquid milk
  • General rapid detection method for micromolecular poisonous and harmful substances in liquid milk
  • General rapid detection method for micromolecular poisonous and harmful substances in liquid milk

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Embodiment Construction

[0054] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0055] The present invention will be further described below with embodiment, but protection scope of the present invention is not limited thereto, protection scope is as the criterion with claim.

[0056] 1. Materials

[0057] 1.1 Main reagents and materials

[0058] Standard substances were purchased from: Dr. Ehrenstorfer, Sigma, Witega and EU pharmacopoeia;

[0059] First prepare the single standard with a concentration of 0.5mg / mL. Chloramphenicol and β in mixed standard solution 2 - 20 ng / mL for agonists, 2000 ng / mL for melamine, natamycin and thyroid inhibitors, 2000 ng / mL for blockers, thyroid inhibitors, quinoxalines, tetracyclines, quinolones, sulfonamides, beta -Lactams, imidazoles, pyridazoles, caffeine, antiparasitics, abamectins, sex hormones, corticosteroids, quinoxalines, mycotoxins, macrolides, non-steroids Anti-inflammat...

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Abstract

The invention discloses a general rapid detection method for micromolecular poisonous and harmful substances in liquid milk. The method is characterized in that the method concretely comprises the following steps: 1, carrying out simple and general extraction and purification on a liquid milk sample to obtain a loading liquid; 2, preparing a mixed standard solution and a matrix addition standard curve; 3, determining the micromolecular poisonous and harmful substances in the liquid milk through UPLC-MS/MS; and 4, carrying out concentration calculation. The method of the invention has the following advantages: the method starts from general characteristics of micromolecular compounds and fully takes the dissolvability, the thermal stability, the acidic and basic stability, the polar strength, the coexistent stability and the like of all the substances into consideration, a general rapid preprocessing technology and a UPLC-MS/MS electro-spray apparatus determination technology are developed, and the substantial efficiency improvement and the detection cost reduction effect are brought to the production process control and food detection work on condition that detection limit requirements are satisfied.

Description

technical field [0001] The invention relates to a detection method of small molecule toxic and harmful substances in liquid milk, in particular to an ultra-trace small molecule toxic and harmful substances in liquid milk (such as veterinary drugs, environmental hormones, biological toxins, pesticides, illegal additives, etc., with a molecular weight less than 1500) as a new rapid and universal detection method. Background technique [0002] Liquid milk (such as fresh milk, fresh milk, yogurt) is an important food for people's daily nutritional intake, but the small molecules contained in liquid milk are toxic and harmful substances such as veterinary drugs, environmental hormones, biological toxins, pesticides, Illegal additives have posed different degrees of safety hazards to its safety. The number of these substances is quite large, and their physical and chemical properties are quite different. In addition, milk contains a high content of matrix interference components s...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 湛嘉王群威钟莺莺陈杰曹苏仙彭锦锋冯睿
Owner NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
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