101 results about "Natamycin" patented technology

The invention relates to the technical field of bioengineering, in particular to streptomyces noursei capable of producing multimycotin B and application of the streptomyces noursei. According to the invention, gene editing is carried out on the nysL gene and the nysGtf gene or a combination thereof in the streptomyces nosella, so that the yield of the polymycotin B in the streptomyces nosella is remarkably improved. According to the streptomyces noursei for producing the multi-fungal element B, the multi-fungal element B can be efficiently synthesized and accumulated by utilizing low-cost raw materials, the yield of the multi-fungal element B is remarkably increased, and the streptomyces noursei can be used for producing the multi-fungal element B or increasing the content ratio of the multi-fungal element B in prepared nystatin. According to the streptomyces noursei and the method for producing the multi-fungal element B by using the streptomyces noursei, provided by the invention, a strain resource and an effective method are provided for producing the multi-fungal element B and producing nysfungin with a high multi-fungal element B content ratio, and the streptomyces noursei has a relatively good application prospect.

An artificial synthesis method for malonyl-CoA and use thereof are provided. By means of heterologous expression of an aminotransferase and a malonyl-CoA reductase, an artificial synthesis pathway for synthesizing malonyl-CoA by using β-alanine (β-ala) as a precursor is constructed as follows: firstly, under catalysis of a transaminase, β-ala transfers amino groups to α-ketonic acid (such as pyruvic acid, oxaloacetic acid, or α-ketoglutaric acid, etc.), to form an intermediate product 3-oxopropanoate and a corresponding amino acid; the 3-oxopropanoate generates malonyl-CoA under the action of the malonyl-CoA reductase. This pathway addresses the defects of the natural malonyl-CoA synthesis pathway, such as low carbon utilization, consumption of energy substance ATP, release of greenhouse gas CO2, and strict regulation of pathway enzymes, a pyruvate dehydrogenase (PDH) and an acetyl-CoA carboxylase (ACC), thereby achieving high yielding of products using malonyl-CoA as a precursor, including flaviolin, octanoic acid, phloroglucinol, pentadecaheptaene, natamycin, and spinosad.

The invention provides bacillus velezensis H5 capable of producing broad-spectrum antibacterial substances and application of the bacillus velezensis H5. The preservation number of the bacillus velezensis H5 in the China General Microbiological Culture Collection Center is CGMCC No.32671. The bacillus velezensis H5 is separated from sea mud in a specific sea area, can generate antibacterial substances with broad-spectrum antibacterial activity, and mainly comprises bacitracin, surfactin and mycostatin. The antibacterial substance of the bacillus velezensis H5 is applied to preservation of perishable fruits such as grapes and the like and mildew prevention of cereals such as corn and the like, and can effectively inhibit spoilage of the perishable fruits and synthesis of mycotoxins such as aflatoxin and the like, and the action effect of the antibacterial substance is obviously superior to that of a food preservative natamycin. The invention provides a novel, green, safe and broad-spectrum antibacterial substance for preservation and fresh-keeping of fruits and mildew prevention of grains, and has important application value.

The invention discloses a fermentation method for improving the yield and purity of natamycin, and belongs to the technical field of microbial application. The method comprises the following steps: in the fermentation process of streptomyces gilvosporeus, changing the type and content of fed-batch carbon sources according to the content of natamycin in fermentation liquor, namely changing the carbon sources fed into the fermentation liquor from glucose to cane sugar, and changing the fermentation pH value from 7.0 to 6.0 at the same time. According to the method disclosed by the invention, the content of the natamycin in the fermentation liquor and the final purity of the natamycin can be remarkably improved, the production cost of the natamycin and the high-purity natamycin is reduced, and the market occupancy of the natamycin is improved.

The invention relates to a composition comprising natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes. The invention further relates to methods employing natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes, preferably said composition, for protecting a plant or plant part, for improving the development and / or yield of an agricultural plant, and for protecting a soil and / or a growth substrate.

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces and component (B) is a compound selected from the group consisting of sulphur, phosphonate, copper hydroxide, copper oxychloride, bicarbonate, Bacillus sp, Trichoderma sp, aureobasidin A, jawsamycin, kasugamycin, natamycin, a polysaccharide elicitor, a plant biostimulant (PBS) comprising an algal extract, a plant extract and / or vinasse, orange oil, and / or tea tree oil. The invention further relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

The invention discloses a streptomyces gilvosporeus engineering strain for high yield of natamycin and a construction method of the streptomyces gilvosporeus engineering strain, and belongs to the technical field of industrial microorganisms. According to the invention, the streptomyces gilvosporeus Z1403 strain with increased natamycin yield and good passage stability is obtained through screening, and the strain is taken as a chassis cell to co-express epwhiG and dasR genes, so that an engineering strain with increased natamycin yield is constructed. The yield of natamycin obtained through shake-flask fermentation of the engineering strain is increased by 64.7% compared with that of an original strain; the yield in a 5L fermentation tank can be 13.5 g.L <-1 >, and the strain has certain industrial application potential.

The invention discloses a streptomyces gilvosporeus strain with high natamycin yield and application thereof, and belongs to the technical field of industrial microorganisms. The phosphate-tolerant natamycin high-yield strain GR-2 is successfully bred by combining protoplast fusion with phosphate tolerance screening, and the yield of natamycin produced by fermentation of the strain in a shake flask can reach 1.81 g / L and is 2.26 times that of an original strain; the fermentation yield in a fermentation tank reaches 13.9 g / L, the passage stability is good, the industrial production requirement can be met, and wide application prospects are achieved.

The invention relates to the technical field of novel fertilizers, and particularly discloses an organic water-soluble fertilizer as well as a preparation method and application thereof. The organic water-soluble fertilizer provided by the invention is prepared from the following components: kitchen waste organic mother liquor, an anti-corrosion synergist, a stress-resistant synergist, a growth-promoting synergist, a dispersing agent and polyethylene glycol-400, the kitchen waste organic mother liquor is prepared by sequentially carrying out wet and hot hydrolysis, amylase enzymolysis, protease enzymolysis and lipase enzymolysis on a kitchen waste solid phase and a kitchen waste liquid phase; the preservative synergist is prepared by mixing a natamycin solution, tea polyphenol and chitosan oligosaccharide; the stress-resistant synergist is prepared by mixing complex enzyme glycoside, a plantain herb extract and gamma-aminobutyric acid; the growth promoting synergist is formed by mixing mineral source potassium fulvate and gluconic acid. The organic water-soluble fertilizer prepared by the invention has good corrosion resistance and product stability, has a good growth promoting effect on crops, and can reduce the number of diseased plants with successive cropping obstacles and improve the yield of the crops in a soil environment with successive cropping obstacles.

The invention provides an antibacterial external medicine for gynecology as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: coupling bornic acid and lysozyme, loading on polydopamine, embedding with polyethylene glycol to prepare a solid dispersion, mixing with a traditional Chinese medicine extract obtained by extracting fructus cnidii, salvia miltiorrhiza, rheum officinale, coptis chinensis and mangnolia officinalis with water, an antibacterial agent obtained by mixing nisin and natamycin, and menthol, adding into a chitosan solution, and uniformly stirring to obtain the antibacterial agent. And adding silver nitrate, poloxamer P407 and poloxamer P188, and uniformly stirring and mixing to obtain the antibacterial external medicine for gynecology. The gynecological antibacterial external medicine prepared by the invention has better antibacterial, bacteriostatic, anti-inflammatory and soothing effects, is high in bioavailability, delayed in medicine release, high in medicine stability and high in patient compliance, and has a wide application prospect.

The invention discloses a feed for feeding cordyceps sinensis host hepialus larvae as well as a preparation method and application of the feed, and relates to the technical field of artificial feeds. The feed comprises the following components in parts by weight: 5-20 parts of beer yeast powder and / or casein, 30-80 parts of wheat germ powder, 20-40 parts of carrot powder, 0.2-5 parts of a modified preservative, 0.05-0.2 part of antibiotics, 0.2-5 parts of vitamins, 0.01-1 part of edible oil, 6-15 parts of agar and 200-400 parts of water. The modified preservative is composed of a chitosan oligosaccharide-natamycin compound subjected to plasma activation treatment and potassium sorbate according to the ratio of 1: 3-1: 5; a modified preservative composed of a chitosan oligosaccharide-natamycin compound subjected to plasma activation treatment and potassium sorbate is adopted, a porous microsphere structure is formed through a specific preparation process, and the contact area of the preservative and the feed is greatly increased. Agar and water form a gel matrix at a specific temperature, and nutritional ingredients and the like are injected into a coaxial micro-fluidic device, so that the preservative effect of the feed is remarkably enhanced.

The invention discloses a method for constructing a high-yield natamycin engineering strain through overexpression of an extracellular transport protein gene pimA and a pathway specific regulation gene pimR, and the high-yield engineering strain is applied to fermentation production of natamycin. The yield of the natamycin can reach 19.49 g / L through fed-batch fermentation in a fermentation tank of 5L of the high-yield strain S.ilvosporeus pimA-pimR, and the yield of the natamycin is improved by 33.12% compared with the yield of a chassis strain S.ilvosporeus TUST01; the production intensity is improved by 33.33% compared with the production intensity of a chassis strain S.ilvosporeus TUST01, and the strain has a relatively high industrial application value.

The invention relates to a composition comprising natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes. The invention further relates to methods employing natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes, preferably said composition, for protecting a plant or plant part, for improving the development and / or yield of an agricultural plant, and for protecting a soil and / or a growth substrate.

The application discloses a kind of efficient natamycin purification methods, comprising the following steps: taking two kinds of dichloromethane, chloroform, acetone, butanone, methanol, ethanol, petroleum ether, n-hexane are mixed to obtain mixed solution, adjust the pH of mixed solution to 6.0-7.0, then natamycin crude product is added to mixed solution and stirred into slurry;Slurry is extracted to obtain that natamycin wet product, and the natamycin wet product after extraction is washed with organic solvent;Natamycin wet product after washing is dried, and natamycin purification product is obtained.Compared with prior art, the present application optimizes the existing natamycin purification process, simplifies the natamycin purification method, reduces the production cost, improves the production efficiency, the product purity can reach more than 98%, and the product yield can reach more than 95%.The application has the advantages of less equipment investment, easy operation, less environmental pollution, simple operation, and is suitable for product industrial production.

A composition (100) for inhibiting growth of microbial contamination is disclosed The composition includes a plurality of volatile antimicrobial components (110) including at least one of potassium sorbate and sorbic acid. The composition further includes a plurality of non-volatile antimicrobial components (120) including natamycin. A weight ratio of the plurality of volatile antimicrobial components to the plurality of non-volatile antimicrobial components is in the range of 10:2 to 10:1. The composition is placed at a distance from a target surface to inhibit microbial growth on the target surface by releasing the plurality of volatile antimicrobial components and the plurality of non-volatile antimicrobial components into a surrounding air. The target surface is exposed to microbial contaminants. moisture and humidity.

The invention relates to the technical field of eggplant manufacturing, in particular to a method for manufacturing primary-color high-anthocyanin eggplants, which comprises the following steps: selecting purple eggplants, cleaning, cutting into long strips, soaking the long-strip-shaped eggplants in a honey solution, and adding citric acid into the honey solution; preparing a color protection solution, adding an enzymolysis blocking agent into the color protection solution, and pouring the eggplants into the color protection solution for later use; preparing a soaking solution, soaking the eggplants in the soaking solution for 10 minutes, taking out the eggplants, performing vacuum packaging on the eggplants to obtain the primary-color high-anthocyanin eggplants, and performing cold storage. According to the method, color protection and synergism of the eggplants are achieved through a dual-targeting mechanism, meanwhile, nutrition preservation of the eggplants is achieved, synergistic repair of cell membranes in the eggplants is achieved through combination of ficin protease and bromelain, and triple-effect removal of fungi, bacteria and biological membranes of the eggplants is achieved through combination of natamycin and subtilin.

The present application belongs to the technical field of food processing, and particularly relates to a biological preservative, a preparation method thereof and application of the biological preservative in baked foods. In view of the poor dispersibility of natamycin in aqueous solution and the poor high-temperature resistance of the biological preservative, the present application first uses supercritical CO2 fluid to prepare soluble soybean polysaccharide microparticles which are small and have uniform particle size distribution, and then uses the microparticles and cotton oligosaccharides to form a double-layer interfacial film on the surface of the natamycin, thereby significantly improving the dispersibility of the natamycin in the biological preservative and greatly improving the high-temperature resistance of the biological preservative. The biological preservative prepared by the present application can be applied to baked foods, and can be sprayed on the surface of the baked foods immediately after baking, thereby effectively prolonging the shelf life of the baked foods at room temperature.

The invention discloses a method for preserving a liquid algae-bacteria composite microbial agent, wherein glucose, urea, potassium dihydrogen phosphate and magnesium sulfate are added to the liquid algae-bacteria composite microbial agent, ethyl paraben, natamycin, uniconazole and glycerol are added after dissolution, pH is adjusted, and a composite microbial agent preservative solution is obtained, which is stored in the dark. The preservation method of the invention utilizes the synergistic effect between the various components to effectively improve the survival rate of the liquid algae-bacteria composite microbial agent. The invention is suitable for preserving the liquid algae-bacteria composite microbial agent, and provides a basis for further application of the liquid algae-bacteria composite microbial agent in production and life.

The invention provides a method for improving spore production of natamycin producing bacteria, belongs to the technical field of streptomyces culture, and mainly provides an FtsZ gene, and the nucleotide sequence of the FtsZ gene is shown as SEQ ID NO.1; the FtsZ gene is applied to promotion of spore production of streptomyces gilvosporeus; the invention provides a genetically engineered bacterium for improving the sporulation quantity of streptomyces gilvosporeus. The genetically engineered bacterium is obtained by introducing an FtsZ gene and an erythromycin strong promoter ermEp into the streptomyces gilvosporeus through a carrier, a strain of streptomyces gilvosporeus F607-F is provided, the streptomyces gilvosporeus F607-F is preserved on October 28, 2024, and the preservation number of the streptomyces gilvosporeus F607-F is CCTCC M 20242352; the invention also provides a solid culture medium for promoting spore production of streptomyces gilvosporeus. The genetically engineered bacterium F607-F provided by the invention has the spore production advantage and can produce natamycin at high yield.

The invention discloses a natamycin-containing water-soluble fertilizer and an application method thereof, and relates to the technical field of water-soluble fertilizers, the natamycin-containing water-soluble fertilizer is prepared from the following raw materials by mass: 65% of nutrient elements, 20% of water, 10% of a stabilizing agent, 10% of a stabilizing agent, and the balance of water. 8% of medium elements; 0.08% of natamycin; 0.1% of EDTA (Ethylene Diamine Tetraacetic Acid) chelated iron, zinc and boron and 0.2% of polysorbate; 0.5% of a surfactant; 0.3% of a stabilizer; 0.02% of vitamin C; 22.8% of water; 1% of a cosolvent; according to the scheme, the problems that an existing water-soluble fertilizer is poor in fungal pollution prevention effect, and after fertilization is finished, products are prone to fungal infection damage, so that the product fertilization effect is poor, and the survival rate is low are solved.

The invention relates to the technical field of food processing, provides a preparation method of a biological preservative and application of the biological preservative to baked food, and solves the problems that in the baking process, the activity of the biological preservative is easily lost, the surface of a product is easily subjected to secondary pollution when the product is cooled, and the dispersion and coating stability is poor due to addition of natamycin; and existing chemical preservatives influence flavor and generate safety burden. Comprising the following steps: S1, preparing a first biological preservative: preparing epsilon-polylysine into an epsilon-polylysine aqueous solution, and adding an antioxidant and a chelating agent to obtain the first biological preservative; s2, preparing a second biological preservative: dispersing natamycin into the first shell layer precursor solution, and homogenizing to form a compact first shell layer on the surface of natamycin microcrystals; and adding the second shell layer precursor solution, homogenizing, forming a second shell layer on the outer surface of the first shell layer, forming a composite shell layer natamycin aqueous dispersion, and obtaining a second biological preservative.

An online solvent recovery device for natamycin drying relates to the technical field of online solvent recovery and is applied to a drying oven, a fixing frame and a mounting frame are fixed to the side face of the drying oven, a condenser is mounted on the fixing frame and communicated with the interior of the drying oven through a guide pipe, and a fan is mounted at the joint of the guide pipe and the drying oven. An air inlet unit is installed at the position, close to the draught fan, of the guide pipe, an air outlet of the condenser communicates with the interior of the drying oven through a backflow pipe, an exhaust pipe is fixedly connected to the backflow pipe in a communicating mode, a multi-way valve is installed at the joint of the exhaust pipe and the backflow pipe, and a solvent storage tank is installed on the inner side of the installation frame. According to the online solvent recovery device for natamycin drying, ethanol can be recovered on line, the drying process is not affected, the consumption of ethanol is greatly reduced, the production cost is saved, and environmental hazards caused when ethanol is discharged along with tail gas are avoided.

The invention discloses effervescent tablet weight-reducing tea and a preparation method thereof, and relates to the technical field of food, and the effervescent tablet weight-reducing tea comprises the following raw materials: cassia seeds, mulberry leaves, lotus leaves, an alkaline material, an acidic material, sucralose, silicon dioxide, leucine, mannitol, citric acid, sodium citrate, neotame, adenosine monophosphate, conjugated linoleic acid powder, a preservative and miscellaneous fruity essence microcapsules. The synthesized preservative is obtained by coating natamycin with a wall material obtained by introducing a feruloyl amide structure to the outer surface of beta-cyclodextrin. The synthesized miscellaneous fruit fragrance essence microcapsule is a product obtained by introducing hindered phenol into a composite wall material formed by modified resistant starch and silicon dioxide and then coating miscellaneous fruit essence. The effervescent tablet weight-reducing tea disclosed by the invention has excellent corrosion resistance and fragrance, is obvious in weight-reducing effect and is worthy of popularization and application.

A method for producing a dry preformed plant growth substrate and to a dry preformed plant growth substrate. The dry preformed growth substrate includes a solid substrate body having a top surface, a bottom surface and an outer surface extending between the top surface and the bottom surface around the solid substrate body, seeds arranged inside the solid substrate body, and the solid substrate body including in a dry state at least one plant-derived component and an adhesive component forming a mixture in a form of a solid substrate body, and the solid substrate body further including natamycin.

The present invention provides an endoscopic cleaning composite disinfectant and a preparation method thereof, belonging to the technical field of disinfectants. It has the following structural formula; the present invention prepares an asymmetric quaternary ammonium base, which is compounded with natamycin to form an endoscopic cleaning composite disinfectant. The preparation method is simple, the reaction conditions are mild, the prepared asymmetric quaternary ammonium base has good disinfection and bactericidal effects, strong decontamination ability, good antibacterial and bacteriostatic effects, and good killing ability against viruses, and has broad application prospects.

The invention relates to the technical field of agriculture, and discloses a plug seedling substrate and a preparation method and application thereof. The plug seedling substrate comprises the following components: tea leaf residues, coco coir, an inorganic porous material and a functional liquid, wherein the functional liquid is prepared from the following components: irdoine, natamycin and polyglutamic acid. According to the plug seedling substrate provided by the invention, the air permeability is provided by the tea leaf residues, the water-retaining property is provided by the coco coir, and the inorganic porous material is used as a substrate framework, so that the requirements of seedlings on the water-retaining property, the air permeability and the root growth space of the substrate are met; meanwhile, the three seedling problems of water management, disease prevention and control and root development are solved; the non-renewable peat is replaced with the tea leaf residues obtained after tea polyphenol extraction and the coco coir, high-value utilization of agricultural waste is achieved, and compared with the non-renewable peat, the raw materials are low in cost and more environmentally friendly.

The invention discloses a nano-robot based on tear driving, a preparation method of the nano-robot and application of the nano-robot in eye drops. The preparation method comprises the following steps: step 1, loading ophthalmic disease treatment drugs such as amphotericin B, voriconazole and natamycin into nano-particles such as polylactic acid-glycolic acid copolymer, chitosan and sodium alginate; 2, coupling urease on the surfaces of the nano-particles to obtain a nano-robot with autonomous movement ability; step 3, performing morphology characterization on the obtained nanometer robot driven by the tear liquid; and step 4, dispersing the prepared tear-driven nano-robot in a buffer solution to prepare the eye drops. The tear-driven nano-robot can utilize urea in tears as fuel to generate cluster motion on the cornea surface, so that the retention time is remarkably prolonged, the drug retention amount is increased, and the treatment effect is improved. The nano-robot eye drops based on tear driving have biocompatibility and treatment safety, and have wide application prospects in the field of ophthalmic local administration.

The present invention discloses a fermentation medium for producing natamycin, relating to the field of microbial fermentation. In addition to a carbon source, a nitrogen source, and a phosphorus source, the fermentation medium contains threonine and biotin. The combined action of threonine and biotin increases the content of propionyl-CoA, a precursor of natamycin. Glutamic acid is added to inhibit the utilization of acetyl-CoA in the tricarboxylic acid cycle, thereby increasing the content of acetyl-CoA, a precursor of natamycin, and promoting the synthesis of natamycin. The present invention is the first to utilize the combined action of threonine and biotin in a natamycin fermentation medium to increase the content of propionyl-CoA, a precursor of natamycin, while simultaneously adding glutamate to inhibit the utilization of acetyl-CoA in the tricarboxylic acid cycle, thereby increasing the content of acetyl-CoA, a precursor of natamycin. These combined actions enhance the metabolic flux of natamycin synthesis, thereby increasing the fermentation yield.

The invention belongs to the technical field of prevention and control of wheat scab, and particularly relates to a bactericidal composition containing bupirimate and orysastrobin or natamycin for preventing and controlling wheat scab. The bactericidal composition is characterized in that effective components of the bactericidal composition are formed by compounding bupirimate and orysastrobin or natamycin, and the mass ratio of bupirimate to orysastrobin is (1-7): (15-1); the mass ratio of bupirimate to natamycin is (1-19): (19-1). The antibacterial action mechanisms of bupirimate and orysastrobin or natamycin in the effective components of the bactericidal composition are different, when the bactericidal composition with the compound formula is applied, target germs can be prevented and removed from different acting sites, generation and development of drug resistance of pathogenic bacteria can be effectively delayed, the drug resistance risk of the agent can be reduced, and the drug resistance risk of the agent can be reduced. And the upgrading period of the bactericidal agent is prolonged.