73 results about "Natamycin" patented technology

The invention relates to the technical field of bioengineering, in particular to streptomyces noursei capable of producing multimycotin B and application of the streptomyces noursei. According to the invention, gene editing is carried out on the nysL gene and the nysGtf gene or a combination thereof in the streptomyces nosella, so that the yield of the polymycotin B in the streptomyces nosella is remarkably improved. According to the streptomyces noursei for producing the multi-fungal element B, the multi-fungal element B can be efficiently synthesized and accumulated by utilizing low-cost raw materials, the yield of the multi-fungal element B is remarkably increased, and the streptomyces noursei can be used for producing the multi-fungal element B or increasing the content ratio of the multi-fungal element B in prepared nystatin. According to the streptomyces noursei and the method for producing the multi-fungal element B by using the streptomyces noursei, provided by the invention, a strain resource and an effective method are provided for producing the multi-fungal element B and producing nysfungin with a high multi-fungal element B content ratio, and the streptomyces noursei has a relatively good application prospect.

The invention provides bacillus velezensis H5 capable of producing broad-spectrum antibacterial substances and application of the bacillus velezensis H5. The preservation number of the bacillus velezensis H5 in the China General Microbiological Culture Collection Center is CGMCC No.32671. The bacillus velezensis H5 is separated from sea mud in a specific sea area, can generate antibacterial substances with broad-spectrum antibacterial activity, and mainly comprises bacitracin, surfactin and mycostatin. The antibacterial substance of the bacillus velezensis H5 is applied to preservation of perishable fruits such as grapes and the like and mildew prevention of cereals such as corn and the like, and can effectively inhibit spoilage of the perishable fruits and synthesis of mycotoxins such as aflatoxin and the like, and the action effect of the antibacterial substance is obviously superior to that of a food preservative natamycin. The invention provides a novel, green, safe and broad-spectrum antibacterial substance for preservation and fresh-keeping of fruits and mildew prevention of grains, and has important application value.

The invention relates to a composition comprising natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes. The invention further relates to methods employing natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes, preferably said composition, for protecting a plant or plant part, for improving the development and / or yield of an agricultural plant, and for protecting a soil and / or a growth substrate.

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces and component (B) is a compound selected from the group consisting of sulphur, phosphonate, copper hydroxide, copper oxychloride, bicarbonate, Bacillus sp, Trichoderma sp, aureobasidin A, jawsamycin, kasugamycin, natamycin, a polysaccharide elicitor, a plant biostimulant (PBS) comprising an algal extract, a plant extract and / or vinasse, orange oil, and / or tea tree oil. The invention further relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

The invention discloses a streptomyces gilvosporeus engineering strain for high yield of natamycin and a construction method of the streptomyces gilvosporeus engineering strain, and belongs to the technical field of industrial microorganisms. According to the invention, the streptomyces gilvosporeus Z1403 strain with increased natamycin yield and good passage stability is obtained through screening, and the strain is taken as a chassis cell to co-express epwhiG and dasR genes, so that an engineering strain with increased natamycin yield is constructed. The yield of natamycin obtained through shake-flask fermentation of the engineering strain is increased by 64.7% compared with that of an original strain; the yield in a 5L fermentation tank can be 13.5 g.L <-1 >, and the strain has certain industrial application potential.

The invention discloses a streptomyces gilvosporeus strain with high natamycin yield and application thereof, and belongs to the technical field of industrial microorganisms. The phosphate-tolerant natamycin high-yield strain GR-2 is successfully bred by combining protoplast fusion with phosphate tolerance screening, and the yield of natamycin produced by fermentation of the strain in a shake flask can reach 1.81 g / L and is 2.26 times that of an original strain; the fermentation yield in a fermentation tank reaches 13.9 g / L, the passage stability is good, the industrial production requirement can be met, and wide application prospects are achieved.

The invention relates to the technical field of novel fertilizers, and particularly discloses an organic water-soluble fertilizer as well as a preparation method and application thereof. The organic water-soluble fertilizer provided by the invention is prepared from the following components: kitchen waste organic mother liquor, an anti-corrosion synergist, a stress-resistant synergist, a growth-promoting synergist, a dispersing agent and polyethylene glycol-400, the kitchen waste organic mother liquor is prepared by sequentially carrying out wet and hot hydrolysis, amylase enzymolysis, protease enzymolysis and lipase enzymolysis on a kitchen waste solid phase and a kitchen waste liquid phase; the preservative synergist is prepared by mixing a natamycin solution, tea polyphenol and chitosan oligosaccharide; the stress-resistant synergist is prepared by mixing complex enzyme glycoside, a plantain herb extract and gamma-aminobutyric acid; the growth promoting synergist is formed by mixing mineral source potassium fulvate and gluconic acid. The organic water-soluble fertilizer prepared by the invention has good corrosion resistance and product stability, has a good growth promoting effect on crops, and can reduce the number of diseased plants with successive cropping obstacles and improve the yield of the crops in a soil environment with successive cropping obstacles.

The invention discloses a feed for feeding cordyceps sinensis host hepialus larvae as well as a preparation method and application of the feed, and relates to the technical field of artificial feeds. The feed comprises the following components in parts by weight: 5-20 parts of beer yeast powder and / or casein, 30-80 parts of wheat germ powder, 20-40 parts of carrot powder, 0.2-5 parts of a modified preservative, 0.05-0.2 part of antibiotics, 0.2-5 parts of vitamins, 0.01-1 part of edible oil, 6-15 parts of agar and 200-400 parts of water. The modified preservative is composed of a chitosan oligosaccharide-natamycin compound subjected to plasma activation treatment and potassium sorbate according to the ratio of 1: 3-1: 5; a modified preservative composed of a chitosan oligosaccharide-natamycin compound subjected to plasma activation treatment and potassium sorbate is adopted, a porous microsphere structure is formed through a specific preparation process, and the contact area of the preservative and the feed is greatly increased. Agar and water form a gel matrix at a specific temperature, and nutritional ingredients and the like are injected into a coaxial micro-fluidic device, so that the preservative effect of the feed is remarkably enhanced.

The invention discloses a method for constructing a high-yield natamycin engineering strain through overexpression of an extracellular transport protein gene pimA and a pathway specific regulation gene pimR, and the high-yield engineering strain is applied to fermentation production of natamycin. The yield of the natamycin can reach 19.49 g / L through fed-batch fermentation in a fermentation tank of 5L of the high-yield strain S.ilvosporeus pimA-pimR, and the yield of the natamycin is improved by 33.12% compared with the yield of a chassis strain S.ilvosporeus TUST01; the production intensity is improved by 33.33% compared with the production intensity of a chassis strain S.ilvosporeus TUST01, and the strain has a relatively high industrial application value.

The invention relates to a composition comprising natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes. The invention further relates to methods employing natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes, preferably said composition, for protecting a plant or plant part, for improving the development and / or yield of an agricultural plant, and for protecting a soil and / or a growth substrate.

The application discloses a kind of efficient natamycin purification methods, comprising the following steps: taking two kinds of dichloromethane, chloroform, acetone, butanone, methanol, ethanol, petroleum ether, n-hexane are mixed to obtain mixed solution, adjust the pH of mixed solution to 6.0-7.0, then natamycin crude product is added to mixed solution and stirred into slurry;Slurry is extracted to obtain that natamycin wet product, and the natamycin wet product after extraction is washed with organic solvent;Natamycin wet product after washing is dried, and natamycin purification product is obtained.Compared with prior art, the present application optimizes the existing natamycin purification process, simplifies the natamycin purification method, reduces the production cost, improves the production efficiency, the product purity can reach more than 98%, and the product yield can reach more than 95%.The application has the advantages of less equipment investment, easy operation, less environmental pollution, simple operation, and is suitable for product industrial production.

A composition (100) for inhibiting growth of microbial contamination is disclosed The composition includes a plurality of volatile antimicrobial components (110) including at least one of potassium sorbate and sorbic acid. The composition further includes a plurality of non-volatile antimicrobial components (120) including natamycin. A weight ratio of the plurality of volatile antimicrobial components to the plurality of non-volatile antimicrobial components is in the range of 10:2 to 10:1. The composition is placed at a distance from a target surface to inhibit microbial growth on the target surface by releasing the plurality of volatile antimicrobial components and the plurality of non-volatile antimicrobial components into a surrounding air. The target surface is exposed to microbial contaminants. moisture and humidity.

The invention relates to the technical field of eggplant manufacturing, in particular to a method for manufacturing primary-color high-anthocyanin eggplants, which comprises the following steps: selecting purple eggplants, cleaning, cutting into long strips, soaking the long-strip-shaped eggplants in a honey solution, and adding citric acid into the honey solution; preparing a color protection solution, adding an enzymolysis blocking agent into the color protection solution, and pouring the eggplants into the color protection solution for later use; preparing a soaking solution, soaking the eggplants in the soaking solution for 10 minutes, taking out the eggplants, performing vacuum packaging on the eggplants to obtain the primary-color high-anthocyanin eggplants, and performing cold storage. According to the method, color protection and synergism of the eggplants are achieved through a dual-targeting mechanism, meanwhile, nutrition preservation of the eggplants is achieved, synergistic repair of cell membranes in the eggplants is achieved through combination of ficin protease and bromelain, and triple-effect removal of fungi, bacteria and biological membranes of the eggplants is achieved through combination of natamycin and subtilin.

The present application belongs to the technical field of food processing, and particularly relates to a biological preservative, a preparation method thereof and application of the biological preservative in baked foods. In view of the poor dispersibility of natamycin in aqueous solution and the poor high-temperature resistance of the biological preservative, the present application first uses supercritical CO2 fluid to prepare soluble soybean polysaccharide microparticles which are small and have uniform particle size distribution, and then uses the microparticles and cotton oligosaccharides to form a double-layer interfacial film on the surface of the natamycin, thereby significantly improving the dispersibility of the natamycin in the biological preservative and greatly improving the high-temperature resistance of the biological preservative. The biological preservative prepared by the present application can be applied to baked foods, and can be sprayed on the surface of the baked foods immediately after baking, thereby effectively prolonging the shelf life of the baked foods at room temperature.

The invention discloses a method for preserving a liquid algae-bacteria composite microbial agent, wherein glucose, urea, potassium dihydrogen phosphate and magnesium sulfate are added to the liquid algae-bacteria composite microbial agent, ethyl paraben, natamycin, uniconazole and glycerol are added after dissolution, pH is adjusted, and a composite microbial agent preservative solution is obtained, which is stored in the dark. The preservation method of the invention utilizes the synergistic effect between the various components to effectively improve the survival rate of the liquid algae-bacteria composite microbial agent. The invention is suitable for preserving the liquid algae-bacteria composite microbial agent, and provides a basis for further application of the liquid algae-bacteria composite microbial agent in production and life.

The invention discloses a natamycin-containing water-soluble fertilizer and an application method thereof, and relates to the technical field of water-soluble fertilizers, the natamycin-containing water-soluble fertilizer is prepared from the following raw materials by mass: 65% of nutrient elements, 20% of water, 10% of a stabilizing agent, 10% of a stabilizing agent, and the balance of water. 8% of medium elements; 0.08% of natamycin; 0.1% of EDTA (Ethylene Diamine Tetraacetic Acid) chelated iron, zinc and boron and 0.2% of polysorbate; 0.5% of a surfactant; 0.3% of a stabilizer; 0.02% of vitamin C; 22.8% of water; 1% of a cosolvent; according to the scheme, the problems that an existing water-soluble fertilizer is poor in fungal pollution prevention effect, and after fertilization is finished, products are prone to fungal infection damage, so that the product fertilization effect is poor, and the survival rate is low are solved.

The invention relates to the technical field of food processing, provides a preparation method of a biological preservative and application of the biological preservative to baked food, and solves the problems that in the baking process, the activity of the biological preservative is easily lost, the surface of a product is easily subjected to secondary pollution when the product is cooled, and the dispersion and coating stability is poor due to addition of natamycin; and existing chemical preservatives influence flavor and generate safety burden. Comprising the following steps: S1, preparing a first biological preservative: preparing epsilon-polylysine into an epsilon-polylysine aqueous solution, and adding an antioxidant and a chelating agent to obtain the first biological preservative; s2, preparing a second biological preservative: dispersing natamycin into the first shell layer precursor solution, and homogenizing to form a compact first shell layer on the surface of natamycin microcrystals; and adding the second shell layer precursor solution, homogenizing, forming a second shell layer on the outer surface of the first shell layer, forming a composite shell layer natamycin aqueous dispersion, and obtaining a second biological preservative.

An online solvent recovery device for natamycin drying relates to the technical field of online solvent recovery and is applied to a drying oven, a fixing frame and a mounting frame are fixed to the side face of the drying oven, a condenser is mounted on the fixing frame and communicated with the interior of the drying oven through a guide pipe, and a fan is mounted at the joint of the guide pipe and the drying oven. An air inlet unit is installed at the position, close to the draught fan, of the guide pipe, an air outlet of the condenser communicates with the interior of the drying oven through a backflow pipe, an exhaust pipe is fixedly connected to the backflow pipe in a communicating mode, a multi-way valve is installed at the joint of the exhaust pipe and the backflow pipe, and a solvent storage tank is installed on the inner side of the installation frame. According to the online solvent recovery device for natamycin drying, ethanol can be recovered on line, the drying process is not affected, the consumption of ethanol is greatly reduced, the production cost is saved, and environmental hazards caused when ethanol is discharged along with tail gas are avoided.

The present invention provides an endoscopic cleaning composite disinfectant and a preparation method thereof, belonging to the technical field of disinfectants. It has the following structural formula; the present invention prepares an asymmetric quaternary ammonium base, which is compounded with natamycin to form an endoscopic cleaning composite disinfectant. The preparation method is simple, the reaction conditions are mild, the prepared asymmetric quaternary ammonium base has good disinfection and bactericidal effects, strong decontamination ability, good antibacterial and bacteriostatic effects, and good killing ability against viruses, and has broad application prospects.

The invention relates to the technical field of agriculture, and discloses a plug seedling substrate and a preparation method and application thereof. The plug seedling substrate comprises the following components: tea leaf residues, coco coir, an inorganic porous material and a functional liquid, wherein the functional liquid is prepared from the following components: irdoine, natamycin and polyglutamic acid. According to the plug seedling substrate provided by the invention, the air permeability is provided by the tea leaf residues, the water-retaining property is provided by the coco coir, and the inorganic porous material is used as a substrate framework, so that the requirements of seedlings on the water-retaining property, the air permeability and the root growth space of the substrate are met; meanwhile, the three seedling problems of water management, disease prevention and control and root development are solved; the non-renewable peat is replaced with the tea leaf residues obtained after tea polyphenol extraction and the coco coir, high-value utilization of agricultural waste is achieved, and compared with the non-renewable peat, the raw materials are low in cost and more environmentally friendly.

The invention discloses a nano-robot based on tear driving, a preparation method of the nano-robot and application of the nano-robot in eye drops. The preparation method comprises the following steps: step 1, loading ophthalmic disease treatment drugs such as amphotericin B, voriconazole and natamycin into nano-particles such as polylactic acid-glycolic acid copolymer, chitosan and sodium alginate; 2, coupling urease on the surfaces of the nano-particles to obtain a nano-robot with autonomous movement ability; step 3, performing morphology characterization on the obtained nanometer robot driven by the tear liquid; and step 4, dispersing the prepared tear-driven nano-robot in a buffer solution to prepare the eye drops. The tear-driven nano-robot can utilize urea in tears as fuel to generate cluster motion on the cornea surface, so that the retention time is remarkably prolonged, the drug retention amount is increased, and the treatment effect is improved. The nano-robot eye drops based on tear driving have biocompatibility and treatment safety, and have wide application prospects in the field of ophthalmic local administration.

The present invention discloses a fermentation medium for producing natamycin, relating to the field of microbial fermentation. In addition to a carbon source, a nitrogen source, and a phosphorus source, the fermentation medium contains threonine and biotin. The combined action of threonine and biotin increases the content of propionyl-CoA, a precursor of natamycin. Glutamic acid is added to inhibit the utilization of acetyl-CoA in the tricarboxylic acid cycle, thereby increasing the content of acetyl-CoA, a precursor of natamycin, and promoting the synthesis of natamycin. The present invention is the first to utilize the combined action of threonine and biotin in a natamycin fermentation medium to increase the content of propionyl-CoA, a precursor of natamycin, while simultaneously adding glutamate to inhibit the utilization of acetyl-CoA in the tricarboxylic acid cycle, thereby increasing the content of acetyl-CoA, a precursor of natamycin. These combined actions enhance the metabolic flux of natamycin synthesis, thereby increasing the fermentation yield.

The invention belongs to the technical field of prevention and control of wheat scab, and particularly relates to a bactericidal composition containing bupirimate and orysastrobin or natamycin for preventing and controlling wheat scab. The bactericidal composition is characterized in that effective components of the bactericidal composition are formed by compounding bupirimate and orysastrobin or natamycin, and the mass ratio of bupirimate to orysastrobin is (1-7): (15-1); the mass ratio of bupirimate to natamycin is (1-19): (19-1). The antibacterial action mechanisms of bupirimate and orysastrobin or natamycin in the effective components of the bactericidal composition are different, when the bactericidal composition with the compound formula is applied, target germs can be prevented and removed from different acting sites, generation and development of drug resistance of pathogenic bacteria can be effectively delayed, the drug resistance risk of the agent can be reduced, and the drug resistance risk of the agent can be reduced. And the upgrading period of the bactericidal agent is prolonged.

This invention discloses a high-efficiency composite protein formaldehyde remover, belonging to the technical field of formaldehyde remover technology. The high-efficiency composite protein formaldehyde remover comprises the following raw materials: modified protein liquid, gelatin, bamboo charcoal powder, fragrance essential oil, 2,6-di-tert-butyl-4-methylphenol, natamycin, calcium carbonate powder, citric acid solution, and papain solution. The high-efficiency composite protein formaldehyde remover obtained by this invention uses non-toxic, environmentally friendly, and inexpensive raw materials, and has the advantages of being non-toxic, odorless, highly efficient in formaldehyde removal, free of secondary pollution, freshening the air, low in cost, and widely applicable.

The invention discloses a method for increasing the fermentation yield of natamycin by adding a composite nitrogen source, and belongs to the technical field of natamycin production. The method comprises the following steps: (1) coating streptomyces gilvosporeus spores in an agar culture medium under a sterile condition, and culturing in an incubator to obtain cultured streptomyces gilvosporeus; (2) putting the cultured streptomyces gilvosporeus into sterile water to prepare a spore suspension, adding the spore suspension into a seed culture medium, and performing fermentation culture to obtain a seed solution; and (3) adding a composite nitrogen source into a fermentation culture medium, inoculating the seed solution into the fermentation culture medium, carrying out fermentation culture in a stirring state, and feeding glucose in the culture process until fermentation is finished, so as to obtain fermentation liquor containing natamycin. By adding the compound nitrogen source, the yield of the natamycin produced by the streptomyces gilvosporeus can be remarkably increased, and the content of the natamycin is up to 22.34 g / L.

The invention discloses an artificial synthesis method and application of malonyl coenzyme A. An artificial synthesis route for synthesizing malonyl-CoA by taking beta-alanine (beta-ala) as a precursor is constructed by heterologous expression of aminotransferase and malonyl-CoA reductase: firstly, under the catalysis of transaminase, amino of beta-ala is transferred to alpha-keto acid (such as pyruvic acid, oxaloacetic acid or alpha-ketoglutaric acid and the like), and then the malonyl-CoA is obtained; an intermediate product 3-oxopropionic acid and corresponding amino acid are formed; and the malonyl-CoA is generated from the 3-oxopropionic acid under the action of the malonyl-CoA reductase. According to the method, the defects existing in a natural malonyl-CoA synthesis route, such as low carbon utilization rate, energy substance ATP consumption, greenhouse gas CO2 release, pyruvate dehydrogenase (PDH) of route enzymes and acetyl-CoA carboxylase (ACC) which are strictly regulated and controlled, are overcome, and high yield of products taking malonyl-CoA as a precursor, including light flavomycin, octanoic acid, phloroglucinol, pentadecheptaene, natamycin, spinosad and the like, is realized.

The invention provides recombinant streptomyces gilvosporeus for producing high-purity natamycin as well as a construction method and application of the recombinant streptomyces gilvosporeus. The recombinant streptomyces gilvosporeus is obtained by introducing a coding gene sgnk of glycosyl transferase, a coding gene sgnd of P450 monooxygenase and a coding gene sgnh of efflux pump protein into the streptomyces gilvosporeus through a carrier, wherein the coding gene sgnk, the coding gene sgnd and the coding gene sgnh are combined with a promoter; according to the recombinant streptomyces gilvosporeus NTIII-KDH, complete conversion of an intermediate is achieved in the fermentation process, the mass purity of natamycin prepared through fermentation reaches up to 99% or above, the streptomyces gilvosporeus NTIII-KDH is preserved in the China Center for Type Culture Collection on October 28, 2024, and the preservation number is CCTCC NO: M 20242353.

The invention discloses a film-forming solution of a fruit and vegetable packaging coating as well as a preparation method and application of the film-forming solution. The film-forming solution comprises natamycin, potassium sorbate, vitamin C, tea polyphenol and a film-forming base material. The preparation method comprises the following steps: heating and dissolving a film-forming base material to obtain a substrate film coating solution; and adding natamycin, potassium sorbate, vitamin C and tea polyphenol into the base film coating liquid, stirring and dissolving to obtain film liquid, forming tiny liquid drops through a spray gun, depositing the tiny liquid drops on the surface of a polyethylene film, and regulating and controlling the release of active matters by utilizing humidity change. The film-forming solution of the fruit and vegetable packaging coating is an active packaging film which has antibacterial and antioxidant effects and is low in cost and high in activity, has a remarkable fresh-keeping effect, can effectively guarantee the quality of fruits and vegetables, can be applied to fresh keeping of the fruits and vegetables, has the advantages of being low in cost, high in adaptability and the like, and is suitable for industrial production and large-scale commercial application.

The invention relates to the technical field of industrial biology, in particular to streptomyces gilvosporeus ZY8 and a fermentation seed and application thereof, the streptomyces gilvosporeus ZY8 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 13, 2024, the preservation number is CGMCC No.32603, and the classification name is streptomyces gilvosporeus. The streptomyces gilvosporeus ZY8 is named as streptomyces gilvosporeus. The streptomyces gilvosporeus ZY8 provided by the invention has a better promoting effect in the aspects of mediating a streptomyces albidosus signal and enhancing the synthesis of natamycin.