5 results about "Natamycin" patented technology

The present application belongs to the technical field of food processing, and particularly relates to a biological preservative, a preparation method thereof and application of the biological preservative in baked foods. In view of the poor dispersibility of natamycin in aqueous solution and the poor high-temperature resistance of the biological preservative, the present application first uses supercritical CO2 fluid to prepare soluble soybean polysaccharide microparticles which are small and have uniform particle size distribution, and then uses the microparticles and cotton oligosaccharides to form a double-layer interfacial film on the surface of the natamycin, thereby significantly improving the dispersibility of the natamycin in the biological preservative and greatly improving the high-temperature resistance of the biological preservative. The biological preservative prepared by the present application can be applied to baked foods, and can be sprayed on the surface of the baked foods immediately after baking, thereby effectively prolonging the shelf life of the baked foods at room temperature.

This invention discloses an engineered bacterium that efficiently catalyzes the synthesis of N-acetyl-natamycin and its applications, belonging to the fields of biocatalysis and enzyme engineering technology. This invention also provides an N-acetyltransferase mutant. Mt GNAT W289A / S121G This mutant, obtained through targeted modification, can catalyze the synthesis of N-acetyl-natamycin using natamycin as a substrate and acetyl-CoA as an acetyl donor. After fed-batch fermentation, the wet cell yield reached 124.8 g / L. In a 1 L reaction system using a 30 g / L catalyst, the N-acetyl-natamycin yield reached 31.2 g / L, with a conversion rate of 90.2%. The wet cells obtained from 1 L of fermentation could catalyze four batches of reactions, yielding a cumulative total of 122.3 g of N-acetyl-natamycin. This invention features mild reaction conditions, high selectivity, and few byproducts, making it suitable for the efficient preparation of N-acetyl-natamycin.

The application discloses a preservative for flower residues and application thereof, and the preservative is composed of natamycin, epsilon-polylysine hydrochloride and potassium sorbate. By performing metagenome sequencing on mildewed flower residues, dominant bacteria causing mildewing and corruption of the flower residues are identified, and the bacteria are separated and purified, thereby obtaining three strains of Penicillium expansum, Penicillium arizonense and Penicillium roqueforti, and two kinds of bacteria of Bacillus subtilis and Raoultella aquatilis. In view of the above-mentioned molds and bacteria, the preservative is selected in a targeted manner, the inhibiting effect of the preservative on the purified bacteria is determined, thereby screening preservative types with good inhibiting effect and small use amount, and then the optimal compound preservative formula is determined through response surface optimization, that is, natamycin 0.001 g / kg, epsilon-polylysine hydrochloride 0.12 g / kg and potassium sorbate 0.166 g / kg. The preservative effect of the compound preservative is verified, and it is found that the compound preservative can obviously inhibit the growth of molds and bacteria in the flower residues.

The application discloses a red ginseng fermentation liquor with multiple effects and a preparation method and application thereof, relates to the technical field of red ginseng fermentation liquor preparation, and comprises the following raw materials: red ginseng, cellulase, pectinase, deionized water, a carbon source, a nitrogen source, inorganic salt, a fermentation strain and modified natamycin; in the application, through synergistic regulation of enzymolysis and microbial fermentation, cellulase and pectinase realize efficient and specific hydrolysis, which not only promotes the dissolution of active ingredients of red ginseng, but also provides sufficient carbon source support for subsequent fermentation; bacillus subtilis, lactic acid bacteria and saccharomyces cerevisiae constitute a stable symbiotic microbial community, which collaboratively converts macromolecular substances in red ginseng into small molecules easy to absorb, and continuously generates various beneficial ingredients, so that the content of functional ingredients and the bioavailability of the red ginseng fermentation liquor are comprehensively improved.

The present invention relates to a method of controlling, limiting or preventing the infestation of plants by Ascomycota plant pathogens, which comprises applying to the plant propagation material a pesticidal combination comprising as active ingredient A a phosphorus-based compound and as active ingredient B a fungicide selected from the group consisting of acibenzolar-S-methyl, fludioxonil, fluazinam, fluindapyr, metconazole, prothioconazole, silthiofam, triticonazole, flutianil, fluorofuram, azoxystrobin, difenoconazole, pyridylcarbamate, ipconazole, tetraconazole, silthiofam, dimethomorph, thiophanate-methyl, natamycin, benzene enofluorocarbostyril, fluazinam, triflupyrrole, high efficiency metalaxyl (metamitron), tetraconazole, isoxazoles, bromofluoride, thiamethoxam, imidacloprid, clothianidin, cyantraniliprole, tioxazofen, flufenoxuron and any mixtures thereof.