43 results about "Natamycin" patented technology

The invention relates to a composition comprising natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes. The invention further relates to methods employing natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes, preferably said composition, for protecting a plant or plant part, for improving the development and / or yield of an agricultural plant, and for protecting a soil and / or a growth substrate.

The invention discloses a streptomyces gilvosporeus engineering strain for high yield of natamycin and a construction method of the streptomyces gilvosporeus engineering strain, and belongs to the technical field of industrial microorganisms. According to the invention, the streptomyces gilvosporeus Z1403 strain with increased natamycin yield and good passage stability is obtained through screening, and the strain is taken as a chassis cell to co-express epwhiG and dasR genes, so that an engineering strain with increased natamycin yield is constructed. The yield of natamycin obtained through shake-flask fermentation of the engineering strain is increased by 64.7% compared with that of an original strain; the yield in a 5L fermentation tank can be 13.5 g.L <-1 >, and the strain has certain industrial application potential.

The invention relates to the technical field of novel fertilizers, and particularly discloses an organic water-soluble fertilizer as well as a preparation method and application thereof. The organic water-soluble fertilizer provided by the invention is prepared from the following components: kitchen waste organic mother liquor, an anti-corrosion synergist, a stress-resistant synergist, a growth-promoting synergist, a dispersing agent and polyethylene glycol-400, the kitchen waste organic mother liquor is prepared by sequentially carrying out wet and hot hydrolysis, amylase enzymolysis, protease enzymolysis and lipase enzymolysis on a kitchen waste solid phase and a kitchen waste liquid phase; the preservative synergist is prepared by mixing a natamycin solution, tea polyphenol and chitosan oligosaccharide; the stress-resistant synergist is prepared by mixing complex enzyme glycoside, a plantain herb extract and gamma-aminobutyric acid; the growth promoting synergist is formed by mixing mineral source potassium fulvate and gluconic acid. The organic water-soluble fertilizer prepared by the invention has good corrosion resistance and product stability, has a good growth promoting effect on crops, and can reduce the number of diseased plants with successive cropping obstacles and improve the yield of the crops in a soil environment with successive cropping obstacles.

The invention discloses a method for constructing a high-yield natamycin engineering strain through overexpression of an extracellular transport protein gene pimA and a pathway specific regulation gene pimR, and the high-yield engineering strain is applied to fermentation production of natamycin. The yield of the natamycin can reach 19.49 g / L through fed-batch fermentation in a fermentation tank of 5L of the high-yield strain S.ilvosporeus pimA-pimR, and the yield of the natamycin is improved by 33.12% compared with the yield of a chassis strain S.ilvosporeus TUST01; the production intensity is improved by 33.33% compared with the production intensity of a chassis strain S.ilvosporeus TUST01, and the strain has a relatively high industrial application value.

The application discloses a kind of efficient natamycin purification methods, comprising the following steps: taking two kinds of dichloromethane, chloroform, acetone, butanone, methanol, ethanol, petroleum ether, n-hexane are mixed to obtain mixed solution, adjust the pH of mixed solution to 6.0-7.0, then natamycin crude product is added to mixed solution and stirred into slurry;Slurry is extracted to obtain that natamycin wet product, and the natamycin wet product after extraction is washed with organic solvent;Natamycin wet product after washing is dried, and natamycin purification product is obtained.Compared with prior art, the present application optimizes the existing natamycin purification process, simplifies the natamycin purification method, reduces the production cost, improves the production efficiency, the product purity can reach more than 98%, and the product yield can reach more than 95%.The application has the advantages of less equipment investment, easy operation, less environmental pollution, simple operation, and is suitable for product industrial production.

A composition (100) for inhibiting growth of microbial contamination is disclosed The composition includes a plurality of volatile antimicrobial components (110) including at least one of potassium sorbate and sorbic acid. The composition further includes a plurality of non-volatile antimicrobial components (120) including natamycin. A weight ratio of the plurality of volatile antimicrobial components to the plurality of non-volatile antimicrobial components is in the range of 10:2 to 10:1. The composition is placed at a distance from a target surface to inhibit microbial growth on the target surface by releasing the plurality of volatile antimicrobial components and the plurality of non-volatile antimicrobial components into a surrounding air. The target surface is exposed to microbial contaminants. moisture and humidity.

The present application belongs to the technical field of food processing, and particularly relates to a biological preservative, a preparation method thereof and application of the biological preservative in baked foods. In view of the poor dispersibility of natamycin in aqueous solution and the poor high-temperature resistance of the biological preservative, the present application first uses supercritical CO2 fluid to prepare soluble soybean polysaccharide microparticles which are small and have uniform particle size distribution, and then uses the microparticles and cotton oligosaccharides to form a double-layer interfacial film on the surface of the natamycin, thereby significantly improving the dispersibility of the natamycin in the biological preservative and greatly improving the high-temperature resistance of the biological preservative. The biological preservative prepared by the present application can be applied to baked foods, and can be sprayed on the surface of the baked foods immediately after baking, thereby effectively prolonging the shelf life of the baked foods at room temperature.

The invention relates to the technical field of food processing, provides a preparation method of a biological preservative and application of the biological preservative to baked food, and solves the problems that in the baking process, the activity of the biological preservative is easily lost, the surface of a product is easily subjected to secondary pollution when the product is cooled, and the dispersion and coating stability is poor due to addition of natamycin; and existing chemical preservatives influence flavor and generate safety burden. Comprising the following steps: S1, preparing a first biological preservative: preparing epsilon-polylysine into an epsilon-polylysine aqueous solution, and adding an antioxidant and a chelating agent to obtain the first biological preservative; s2, preparing a second biological preservative: dispersing natamycin into the first shell layer precursor solution, and homogenizing to form a compact first shell layer on the surface of natamycin microcrystals; and adding the second shell layer precursor solution, homogenizing, forming a second shell layer on the outer surface of the first shell layer, forming a composite shell layer natamycin aqueous dispersion, and obtaining a second biological preservative.

An online solvent recovery device for natamycin drying relates to the technical field of online solvent recovery and is applied to a drying oven, a fixing frame and a mounting frame are fixed to the side face of the drying oven, a condenser is mounted on the fixing frame and communicated with the interior of the drying oven through a guide pipe, and a fan is mounted at the joint of the guide pipe and the drying oven. An air inlet unit is installed at the position, close to the draught fan, of the guide pipe, an air outlet of the condenser communicates with the interior of the drying oven through a backflow pipe, an exhaust pipe is fixedly connected to the backflow pipe in a communicating mode, a multi-way valve is installed at the joint of the exhaust pipe and the backflow pipe, and a solvent storage tank is installed on the inner side of the installation frame. According to the online solvent recovery device for natamycin drying, ethanol can be recovered on line, the drying process is not affected, the consumption of ethanol is greatly reduced, the production cost is saved, and environmental hazards caused when ethanol is discharged along with tail gas are avoided.

The invention discloses a nano-robot based on tear driving, a preparation method of the nano-robot and application of the nano-robot in eye drops. The preparation method comprises the following steps: step 1, loading ophthalmic disease treatment drugs such as amphotericin B, voriconazole and natamycin into nano-particles such as polylactic acid-glycolic acid copolymer, chitosan and sodium alginate; 2, coupling urease on the surfaces of the nano-particles to obtain a nano-robot with autonomous movement ability; step 3, performing morphology characterization on the obtained nanometer robot driven by the tear liquid; and step 4, dispersing the prepared tear-driven nano-robot in a buffer solution to prepare the eye drops. The tear-driven nano-robot can utilize urea in tears as fuel to generate cluster motion on the cornea surface, so that the retention time is remarkably prolonged, the drug retention amount is increased, and the treatment effect is improved. The nano-robot eye drops based on tear driving have biocompatibility and treatment safety, and have wide application prospects in the field of ophthalmic local administration.

This invention discloses a high-efficiency composite protein formaldehyde remover, belonging to the technical field of formaldehyde remover technology. The high-efficiency composite protein formaldehyde remover comprises the following raw materials: modified protein liquid, gelatin, bamboo charcoal powder, fragrance essential oil, 2,6-di-tert-butyl-4-methylphenol, natamycin, calcium carbonate powder, citric acid solution, and papain solution. The high-efficiency composite protein formaldehyde remover obtained by this invention uses non-toxic, environmentally friendly, and inexpensive raw materials, and has the advantages of being non-toxic, odorless, highly efficient in formaldehyde removal, free of secondary pollution, freshening the air, low in cost, and widely applicable.

The invention discloses a method for increasing the fermentation yield of natamycin by adding a composite nitrogen source, and belongs to the technical field of natamycin production. The method comprises the following steps: (1) coating streptomyces gilvosporeus spores in an agar culture medium under a sterile condition, and culturing in an incubator to obtain cultured streptomyces gilvosporeus; (2) putting the cultured streptomyces gilvosporeus into sterile water to prepare a spore suspension, adding the spore suspension into a seed culture medium, and performing fermentation culture to obtain a seed solution; and (3) adding a composite nitrogen source into a fermentation culture medium, inoculating the seed solution into the fermentation culture medium, carrying out fermentation culture in a stirring state, and feeding glucose in the culture process until fermentation is finished, so as to obtain fermentation liquor containing natamycin. By adding the compound nitrogen source, the yield of the natamycin produced by the streptomyces gilvosporeus can be remarkably increased, and the content of the natamycin is up to 22.34 g / L.

The invention discloses an artificial synthesis method and application of malonyl coenzyme A. An artificial synthesis route for synthesizing malonyl-CoA by taking beta-alanine (beta-ala) as a precursor is constructed by heterologous expression of aminotransferase and malonyl-CoA reductase: firstly, under the catalysis of transaminase, amino of beta-ala is transferred to alpha-keto acid (such as pyruvic acid, oxaloacetic acid or alpha-ketoglutaric acid and the like), and then the malonyl-CoA is obtained; an intermediate product 3-oxopropionic acid and corresponding amino acid are formed; and the malonyl-CoA is generated from the 3-oxopropionic acid under the action of the malonyl-CoA reductase. According to the method, the defects existing in a natural malonyl-CoA synthesis route, such as low carbon utilization rate, energy substance ATP consumption, greenhouse gas CO2 release, pyruvate dehydrogenase (PDH) of route enzymes and acetyl-CoA carboxylase (ACC) which are strictly regulated and controlled, are overcome, and high yield of products taking malonyl-CoA as a precursor, including light flavomycin, octanoic acid, phloroglucinol, pentadecheptaene, natamycin, spinosad and the like, is realized.

The invention relates to the field of apple preservation, in particular to a natamycin preservative and a preservation determination method aiming at fungal disease water rot disease. The preservative is natamycin with the concentration of 50 mg / L to 300 mg / L; the effective concentration is 50 mg / L-300 mg / L, and the compound is sprayed to a preservative film and is combined in palm wax or sodium alginate or passes through a slow release bag. The device is arranged in a packaging piece. The inhibition effect is better along with the increase of the concentration in the range of 300mg / L, but the inhibition effect is weak when the concentration is lower than 5mg / L. According to the determination of the physiological indexes of the apple fruits, the natamycin solution does not generate negative influence on the quality of the fruits while inhibiting bacteria.

The invention discloses a diamond polishing solution and a preparation method and a chemical mechanical polishing method thereof, and the diamond polishing solution comprises the following components by total mass of 100%: 1-10% of polyvinyl alcohol, 1-5% of natamycin, 0.5-3% of organic amine ester, 0.5-3% of diamond micro powder, and the balance of pure water. The diamond polishing solution provided by the invention has relatively good compatibility, and through multi-space structure obstruction and crystal electrostatic repulsion, the phenomena of temperature change and grinding agglomeration in the grinding process are remarkably reduced, the problem of longitudinal lines on the surface of a wafer is solved, the grinding repeatability and the one-time yield are guaranteed, and the production efficiency is improved. And the application requirements of the polishing process of the ultra-thin silicon carbide and monocrystalline silicon wafer with the thickness of 150-250 microns can be fully met.

The invention discloses cucumis metuliferus sparkling water and a preparation method thereof, and relates to the field of beverage processing, the preparation method comprises the following steps: S1, cucumis metuliferus pulp and a citric acid solution are mixed, subjected to ultrasonic treatment and squeezed, and squeezed juice is obtained; s2, adding a chitosan solution, stirring, standing, filtering, and carrying out vacuum degassing to obtain horned melon juice; s3, mixing the cucumis metuliferus normal juice, water, a sweetening agent, lemon concentrated juice and honey, adding cucumis metuliferus essence, menthol and vitamin E, and stirring to obtain blended liquid; s4, adding a compound stabilizer, stirring, and injecting carbon dioxide to obtain a bubble water crude product; and S5, adding a rosemary extract and natamycin, performing sterile filling, performing pasteurization, and performing cooling so as to obtain a horned melon sparkling water finished product. The problem of juice residue caused by insufficient crushing of the causal capsule structure can be avoided, so that the waste of raw materials is reduced, and the stability of a product combined with bubble water is further enhanced.

The invention discloses a rice husk-based nano antibacterial edible film and a preparation method thereof, and belongs to the technical field of food packaging materials. The edible film comprises the following components: rice hull nanofibers; chitosan is added; plant essential oil; starch; a plasticizer and a compound antibacterial agent. Wherein the grain size of the rice husk nanofibers is 50-900 nm. The compound antibacterial agent is prepared from natamycin and polylysine. Meanwhile, the invention further provides a preparation method of the edible film. The edible film provided by the invention has excellent antibacterial property, mechanical property and barrier property, and is beneficial to prolonging the shelf life of food. Meanwhile, the edible film is made of rice hull fibers, so that the utilization rate of rice hulls can be increased, and environmental pollution caused by rice hull treatment can be reduced.

This invention relates to a method of controlling, limiting or preventing an infestation of a plant by Oomycetes, which comprises applying to propagation material of said plant, a pesticidal combination comprising a phosphorus-based compound as active ingredient A, and an active ingredient B selected from acibenzolar-S-methyl, fludioxonil, fluxapyroxad, inpyrfluxam, mefentrifluconazole, prothioconazole, sedaxane, triticonazole, oxathiapiprolin, fluoxapiprolin, azoxystrobin, difenoconazole, fluopyram, ipconazole, metyltetraprole, silthiofam, dimethomorph, thiophanate-methyl, natamycin, benzovindiflupyr, pydiflumetofen, picarbutrazox, isocycloseram, cyclobutrifluram, broflanilide, thiamethoxam, imidacloprid, clothianidin, cyantraniliprole, chlorantraniliprole, tetraniliprole, fipronil, and any mixture thereof.

The present application relates to the technical field of cigar tobacco processing, and particularly relates to a method for preventing mildew of fermented cigar tobacco. The method comprises the following steps: first, spraying a rhamnolipid solution to the surface of the cigar tobacco, and then placing the cigar tobacco in a constant temperature and humidity environment for fermentation. In the present application, the rhamnolipid is mainly used to achieve the mildew prevention effect, and the mechanism is mainly to damage the cell wall and cell membrane. Both fungi and bacteria have cell walls and cell membranes, so compared with natamycin in the prior art which can only act on fungi, the present application has higher mildew prevention effect.

The invention belongs to the field of fresh-keeping processing of agricultural products, and particularly relates to a green fresh-keeping agent based on a natamycin nano system as well as a preparation method and application of the green fresh-keeping agent. According to the preservative provided by the invention, a natural product natamycin obtained by fungus fermentation is used as a bacteriostatic active substance, sorghum prolamine is combined in a solution system, and a stabilizer is added, so that the green preservative with a stable nano-dispersion system is prepared. The fruit and vegetable preservative is simple in production process, low in cost, green and safe, and can be applied to fruits and vegetables such as tomatoes, grapes, blueberries, strawberries and cucumbers as a novel fruit and vegetable preservative.

The present application relates to the field of agricultural technology, and discloses a kind of plug seedling substrate and its preparation method and application.The plug seedling substrate includes the following components: tea dregs, coconut bran, inorganic porous material and functional liquid;Wherein, the functional liquid includes the following components: ikdoine, natamycin and polyglutamic acid.The plug seedling substrate provided by the present application provides air permeability with tea dregs, provides water retention with coconut bran, and uses inorganic porous material as the substrate framework, meeting the requirements of seedlings for substrate water retention, air permeability and root growth space, by adding functional liquid containing active ingredients ikdoine, natamycin and polyglutamic acid, while solving the three major problems of water management, disease control and root development.Plug seedling.The tea dregs after extracting tea polyphenols and coconut bran replace non-renewable peat, realizing high-value utilization of agricultural waste, and compared with non-renewable peat, the raw material cost is low and more environmentally friendly.

This invention relates to the field of stainless steel plate technology, and discloses a stainless steel plate based on water-based paint and its application. The stainless steel plate is made by spraying water-based paint onto the surface of a stainless steel plate. The water-based paint includes the following raw materials: methyl methacrylate, butyl acrylate, hydroxyethyl methacrylate, acrylic acid, antifouling modifier, emulsifier, deionized water, initiator, inorganic modifier additive, pigments and fillers, film-forming aid, leveling agent, and defoamer. The antifouling modifier is prepared by preparing a hydroxyl fluorosilicone oil derivative containing siloxane segments, fluorinated segments, and long alkyl chains. The inorganic modifier additive is prepared by chemically linking natamycin to the surface of titanium dioxide. The stainless steel plate prepared by this invention has excellent antifouling and antibacterial and antifungal properties, enhancing its market competitiveness.

The invention relates to a novel amorphous natamycin, compositions comprising it, the use of amorphous natamycin or of the compositions in food, feed, crop or pharmaceutical products, and a manufacturing process to obtain the amorphous natamycin.

A fungicidal composition, the fungicidal composition comprising a component A, the component A comprising a cyclic ester peptide gold basidiosin A or a stereoisomer thereof; and a component B, wherein the component B comprises spring daily mycin or natamycin. A method of controlling or preventing a phytopathogenic disease or a phytopathogenic fungus on a plant or on propagation material thereof, the method comprising applying the composition to the plant, locus or propagation material thereof. The invention also relates to the use of the composition as a fungicide.

The invention belongs to the technical field of pesticides, and particularly relates to a combined synergistic bactericidal composition for preventing and treating avocado root rot. The invention relates to a combined synergistic bactericidal composition. Effective components of the combined synergistic bactericidal composition are formed by binary compounding of fluoride ether bacteria amide and natamycin, when the fluoride ether bacteria amide and the natamycin are subjected to binary compounding, the synergistic coefficient of the fluoride ether bacteria amide and the natamycin on phytophthora cinnamomum is greater than 1.5, namely, the fluoride ether bacteria amide and the natamycin have a certain synergistic effect. Particularly, when the mass ratio of the fluoride ether bacteria amide to the natamycin is 8: 1, the SR value reaches 11.2394, and the synergistic effect is most remarkable. Therefore, the combined synergistic bactericidal composition disclosed by the invention is beneficial to improving the control effect on the avocado root rot, and can be developed into a high-efficiency bactericide for controlling the avocado root rot. In addition, the combined synergistic bactericidal composition is formed by binary compounding of the two effective components, development of drug resistance of pathogenic bacteria can be delayed, and the risk of drug resistance is reduced.

The present application belongs to the technical field of pesticides, and particularly relates to a fungicidal composition for preventing and treating oil olive root rot. The effective components of the fungicidal composition are formed by binary compounding of fluoximide and berberine or natamycin. When fluoximide and berberine or natamycin are binary compounded, the synergistic coefficient of the fungicidal composition to phytophthora cinnamomi is greater than 1.5, that is, the fungicidal composition exhibits a certain synergistic effect. In particular, when the mass ratio of fluoximide to natamycin is 8:1, the SR value of the fungicidal composition reaches 11.2394, and the synergistic effect is the most significant. Therefore, the fungicidal composition can help to improve the prevention and treatment effect on oil olive root rot, and can be used for developing a high-efficiency fungicide for preventing and treating oil olive root rot. In addition, the fungicidal composition is formed by binary compounding of two effective components, can delay the development of pathogen resistance, and reduce the risk of drug resistance.

This invention relates to a method of controlling, limiting or preventing an infestation of a plant by Oomycetes, which comprises applying to propagation material of said plant, a pesticidal combination comprising a phosphorus-based compound as active ingredient A, and an active ingredient B selected from acibenzolar-S-methyl, fludioxonil, fluxapyroxad, inpyrfluxam, mefentrifluconazole, prothioconazole, sedaxane, triticonazole, oxathiapiprolin, fluoxapiprolin, azoxystrobin, difenoconazole, fluopyram, ipconazole, metyltetraprole, silthiofam, dimethomorph, thiophanate-methyl, natamycin, benzovindiflupyr, pydiflumetofen, picarbutrazox, isocycloseram, cyclobutrifluram, broflanilide, thiamethoxam, imidacloprid, clothianidin, cyantraniliprole, chlorantraniliprole, tetraniliprole, fipronil, and any mixture thereof.

The present application relates to natamycin production technical field, specifically to a method for improving natamycin fermentation yield, comprising the following steps: shake flask seed culture: under sterile conditions, brown yellow spore streptomyces spores are coated on agar medium, and placed in a culture box for culture, then the cultured brown yellow spore streptomyces is prepared into spore suspension, and the spore suspension is taken and added into a triangular flask of seed culture medium for culture; fermentation tank fermentation culture: the cultured seed liquid is inoculated into a fermentation culture medium comprising proteose peptone, yeast powder, sodium chloride, magnesium sulfate, glucose, betaine, ampicillin, sodium succinate for stirring culture, and glucose is added during the culture until the fermentation is completed, and natamycin is obtained. The present application significantly improves the yield of brown yellow spore streptomyces producing natamycin by adding betaine, ampicillin and sodium succinate in the fermentation culture medium; the production method is simple to operate and is conducive to large-scale industrialization.

The invention belongs to the technical field of biopesticides, and discloses application of natamycin in prevention and treatment of poplar canker. The EC50 value of the natamycin on the growth of botryosphaeria dothidea hyphae causing the poplar canker is 2.1500 g / mL through a colony diameter measurement method, and an optimized formula (containing 4% of an SDS wetting agent and 6% of an NNO dispersing agent) of the 20% natamycin wettable powder is further developed. In-vitro branch and forest tests prove that a 2000-fold solution of the wettable powder can reduce the disease index of branches to 12.10 (the control effect reaches 78.64%); the forest control effect of 500-fold liquid of the wettable powder can reach 69.93% 7 days after secondary pesticide application, and the forest control effect of 500-fold liquid of the wettable powder can be stabilized at 60.6% 7 days after third pesticide application. The natural product natamycin which has the characteristics of low toxicity, easiness in degradation and environment friendliness is utilized, and a new scheme is provided for green prevention and control of poplar canker.