18 results about "Natamycin" patented technology

The invention discloses a method for constructing a high-yield natamycin engineering strain through overexpression of an extracellular transport protein gene pimA and a pathway specific regulation gene pimR, and the high-yield engineering strain is applied to fermentation production of natamycin. The yield of the natamycin can reach 19.49 g / L through fed-batch fermentation in a fermentation tank of 5L of the high-yield strain S.ilvosporeus pimA-pimR, and the yield of the natamycin is improved by 33.12% compared with the yield of a chassis strain S.ilvosporeus TUST01; the production intensity is improved by 33.33% compared with the production intensity of a chassis strain S.ilvosporeus TUST01, and the strain has a relatively high industrial application value.

The present application belongs to the technical field of food processing, and particularly relates to a biological preservative, a preparation method thereof and application of the biological preservative in baked foods. In view of the poor dispersibility of natamycin in aqueous solution and the poor high-temperature resistance of the biological preservative, the present application first uses supercritical CO2 fluid to prepare soluble soybean polysaccharide microparticles which are small and have uniform particle size distribution, and then uses the microparticles and cotton oligosaccharides to form a double-layer interfacial film on the surface of the natamycin, thereby significantly improving the dispersibility of the natamycin in the biological preservative and greatly improving the high-temperature resistance of the biological preservative. The biological preservative prepared by the present application can be applied to baked foods, and can be sprayed on the surface of the baked foods immediately after baking, thereby effectively prolonging the shelf life of the baked foods at room temperature.

The invention relates to the technical field of food processing, provides a preparation method of a biological preservative and application of the biological preservative to baked food, and solves the problems that in the baking process, the activity of the biological preservative is easily lost, the surface of a product is easily subjected to secondary pollution when the product is cooled, and the dispersion and coating stability is poor due to addition of natamycin; and existing chemical preservatives influence flavor and generate safety burden. Comprising the following steps: S1, preparing a first biological preservative: preparing epsilon-polylysine into an epsilon-polylysine aqueous solution, and adding an antioxidant and a chelating agent to obtain the first biological preservative; s2, preparing a second biological preservative: dispersing natamycin into the first shell layer precursor solution, and homogenizing to form a compact first shell layer on the surface of natamycin microcrystals; and adding the second shell layer precursor solution, homogenizing, forming a second shell layer on the outer surface of the first shell layer, forming a composite shell layer natamycin aqueous dispersion, and obtaining a second biological preservative.

The invention discloses a method for increasing the fermentation yield of natamycin by adding a composite nitrogen source, and belongs to the technical field of natamycin production. The method comprises the following steps: (1) coating streptomyces gilvosporeus spores in an agar culture medium under a sterile condition, and culturing in an incubator to obtain cultured streptomyces gilvosporeus; (2) putting the cultured streptomyces gilvosporeus into sterile water to prepare a spore suspension, adding the spore suspension into a seed culture medium, and performing fermentation culture to obtain a seed solution; and (3) adding a composite nitrogen source into a fermentation culture medium, inoculating the seed solution into the fermentation culture medium, carrying out fermentation culture in a stirring state, and feeding glucose in the culture process until fermentation is finished, so as to obtain fermentation liquor containing natamycin. By adding the compound nitrogen source, the yield of the natamycin produced by the streptomyces gilvosporeus can be remarkably increased, and the content of the natamycin is up to 22.34 g / L.

The invention relates to the field of apple preservation, in particular to a natamycin preservative and a preservation determination method aiming at fungal disease water rot disease. The preservative is natamycin with the concentration of 50 mg / L to 300 mg / L; the effective concentration is 50 mg / L-300 mg / L, and the compound is sprayed to a preservative film and is combined in palm wax or sodium alginate or passes through a slow release bag. The device is arranged in a packaging piece. The inhibition effect is better along with the increase of the concentration in the range of 300mg / L, but the inhibition effect is weak when the concentration is lower than 5mg / L. According to the determination of the physiological indexes of the apple fruits, the natamycin solution does not generate negative influence on the quality of the fruits while inhibiting bacteria.

The invention discloses a diamond polishing solution and a preparation method and a chemical mechanical polishing method thereof, and the diamond polishing solution comprises the following components by total mass of 100%: 1-10% of polyvinyl alcohol, 1-5% of natamycin, 0.5-3% of organic amine ester, 0.5-3% of diamond micro powder, and the balance of pure water. The diamond polishing solution provided by the invention has relatively good compatibility, and through multi-space structure obstruction and crystal electrostatic repulsion, the phenomena of temperature change and grinding agglomeration in the grinding process are remarkably reduced, the problem of longitudinal lines on the surface of a wafer is solved, the grinding repeatability and the one-time yield are guaranteed, and the production efficiency is improved. And the application requirements of the polishing process of the ultra-thin silicon carbide and monocrystalline silicon wafer with the thickness of 150-250 microns can be fully met.

This invention relates to a method of controlling, limiting or preventing an infestation of a plant by Oomycetes, which comprises applying to propagation material of said plant, a pesticidal combination comprising a phosphorus-based compound as active ingredient A, and an active ingredient B selected from acibenzolar-S-methyl, fludioxonil, fluxapyroxad, inpyrfluxam, mefentrifluconazole, prothioconazole, sedaxane, triticonazole, oxathiapiprolin, fluoxapiprolin, azoxystrobin, difenoconazole, fluopyram, ipconazole, metyltetraprole, silthiofam, dimethomorph, thiophanate-methyl, natamycin, benzovindiflupyr, pydiflumetofen, picarbutrazox, isocycloseram, cyclobutrifluram, broflanilide, thiamethoxam, imidacloprid, clothianidin, cyantraniliprole, chlorantraniliprole, tetraniliprole, fipronil, and any mixture thereof.

The present application relates to the technical field of cigar tobacco processing, and particularly relates to a method for preventing mildew of fermented cigar tobacco. The method comprises the following steps: first, spraying a rhamnolipid solution to the surface of the cigar tobacco, and then placing the cigar tobacco in a constant temperature and humidity environment for fermentation. In the present application, the rhamnolipid is mainly used to achieve the mildew prevention effect, and the mechanism is mainly to damage the cell wall and cell membrane. Both fungi and bacteria have cell walls and cell membranes, so compared with natamycin in the prior art which can only act on fungi, the present application has higher mildew prevention effect.

The present application relates to the field of agricultural technology, and discloses a kind of plug seedling substrate and its preparation method and application.The plug seedling substrate includes the following components: tea dregs, coconut bran, inorganic porous material and functional liquid;Wherein, the functional liquid includes the following components: ikdoine, natamycin and polyglutamic acid.The plug seedling substrate provided by the present application provides air permeability with tea dregs, provides water retention with coconut bran, and uses inorganic porous material as the substrate framework, meeting the requirements of seedlings for substrate water retention, air permeability and root growth space, by adding functional liquid containing active ingredients ikdoine, natamycin and polyglutamic acid, while solving the three major problems of water management, disease control and root development.Plug seedling.The tea dregs after extracting tea polyphenols and coconut bran replace non-renewable peat, realizing high-value utilization of agricultural waste, and compared with non-renewable peat, the raw material cost is low and more environmentally friendly.

A fungicidal composition, the fungicidal composition comprising a component A, the component A comprising a cyclic ester peptide gold basidiosin A or a stereoisomer thereof; and a component B, wherein the component B comprises spring daily mycin or natamycin. A method of controlling or preventing a phytopathogenic disease or a phytopathogenic fungus on a plant or on propagation material thereof, the method comprising applying the composition to the plant, locus or propagation material thereof. The invention also relates to the use of the composition as a fungicide.

The invention belongs to the technical field of pesticides, and particularly relates to a combined synergistic bactericidal composition for preventing and treating avocado root rot. The invention relates to a combined synergistic bactericidal composition. Effective components of the combined synergistic bactericidal composition are formed by binary compounding of fluoride ether bacteria amide and natamycin, when the fluoride ether bacteria amide and the natamycin are subjected to binary compounding, the synergistic coefficient of the fluoride ether bacteria amide and the natamycin on phytophthora cinnamomum is greater than 1.5, namely, the fluoride ether bacteria amide and the natamycin have a certain synergistic effect. Particularly, when the mass ratio of the fluoride ether bacteria amide to the natamycin is 8: 1, the SR value reaches 11.2394, and the synergistic effect is most remarkable. Therefore, the combined synergistic bactericidal composition disclosed by the invention is beneficial to improving the control effect on the avocado root rot, and can be developed into a high-efficiency bactericide for controlling the avocado root rot. In addition, the combined synergistic bactericidal composition is formed by binary compounding of the two effective components, development of drug resistance of pathogenic bacteria can be delayed, and the risk of drug resistance is reduced.

The present application relates to natamycin production technical field, specifically to a method for improving natamycin fermentation yield, comprising the following steps: shake flask seed culture: under sterile conditions, brown yellow spore streptomyces spores are coated on agar medium, and placed in a culture box for culture, then the cultured brown yellow spore streptomyces is prepared into spore suspension, and the spore suspension is taken and added into a triangular flask of seed culture medium for culture; fermentation tank fermentation culture: the cultured seed liquid is inoculated into a fermentation culture medium comprising proteose peptone, yeast powder, sodium chloride, magnesium sulfate, glucose, betaine, ampicillin, sodium succinate for stirring culture, and glucose is added during the culture until the fermentation is completed, and natamycin is obtained. The present application significantly improves the yield of brown yellow spore streptomyces producing natamycin by adding betaine, ampicillin and sodium succinate in the fermentation culture medium; the production method is simple to operate and is conducive to large-scale industrialization.

The utility model relates to the field of filtering devices, in particular to a filtering device for natamycin extraction, which comprises a plurality of plate frames, each plate frame comprises a middle plate, a middle pipe is rotatably arranged in the center of each middle plate, ring frames are symmetrically propped against the two sides of each middle plate, and filtering cloth is fixed between the outer side edges of the two ring frames and the outer end of the middle pipe on the same side. A liquid leakage hole is formed between the filter cloth at the lower ends of the two ring frames and the middle plate in a penetrating manner, and a plurality of ribs are arranged on the two side surfaces of the middle plate. Opening grooves are formed in the positions, corresponding to the middle plate, of the upper ends of the two annular frames, and power wheels are rotationally arranged at the upper ends of the two annular frames and located in the opening grooves to conduct transmission on the middle plate. According to the utility model, the filter cake adhered to the filter cloth can be quickly separated.

The application discloses a preservative for flower residues and application thereof, and the preservative is composed of natamycin, epsilon-polylysine hydrochloride and potassium sorbate. By performing metagenome sequencing on mildewed flower residues, dominant bacteria causing mildewing and corruption of the flower residues are identified, and the bacteria are separated and purified, thereby obtaining three strains of Penicillium expansum, Penicillium arizonense and Penicillium roqueforti, and two kinds of bacteria of Bacillus subtilis and Raoultella aquatilis. In view of the above-mentioned molds and bacteria, the preservative is selected in a targeted manner, the inhibiting effect of the preservative on the purified bacteria is determined, thereby screening preservative types with good inhibiting effect and small use amount, and then the optimal compound preservative formula is determined through response surface optimization, that is, natamycin 0.001 g / kg, epsilon-polylysine hydrochloride 0.12 g / kg and potassium sorbate 0.166 g / kg. The preservative effect of the compound preservative is verified, and it is found that the compound preservative can obviously inhibit the growth of molds and bacteria in the flower residues.

The invention discloses an application of terpinen-4-ol in preparation of a medicine for treating fusarium solani keratitis. The active ingredients of the medicine are composed of terpinen-4-ol and natamycin. The terpinen-4-ol can inhibit spore germination and mycelial growth of the fusarium solani, and can effectively inhibit the activity of the fusarium solani under the contact condition of 1-10 minutes. The combined use of the terpinen-4-ol and the natamycin has additive bacteriostatic activity, and the terpinen-4-ol can effectively enhance the bacteriostatic activity of the natamycin to fusarium solani under the condition of short-time treatment. The invention provides an alternative medicine and a new medication scheme for the treatment of fusarium solani keratitis.

The application discloses a red ginseng fermentation liquor with multiple effects and a preparation method and application thereof, relates to the technical field of red ginseng fermentation liquor preparation, and comprises the following raw materials: red ginseng, cellulase, pectinase, deionized water, a carbon source, a nitrogen source, inorganic salt, a fermentation strain and modified natamycin; in the application, through synergistic regulation of enzymolysis and microbial fermentation, cellulase and pectinase realize efficient and specific hydrolysis, which not only promotes the dissolution of active ingredients of red ginseng, but also provides sufficient carbon source support for subsequent fermentation; bacillus subtilis, lactic acid bacteria and saccharomyces cerevisiae constitute a stable symbiotic microbial community, which collaboratively converts macromolecular substances in red ginseng into small molecules easy to absorb, and continuously generates various beneficial ingredients, so that the content of functional ingredients and the bioavailability of the red ginseng fermentation liquor are comprehensively improved.

The present invention relates to a method of controlling, limiting or preventing the infestation of plants by Ascomycota plant pathogens, which comprises applying to the plant propagation material a pesticidal combination comprising as active ingredient A a phosphorus-based compound and as active ingredient B a fungicide selected from the group consisting of acibenzolar-S-methyl, fludioxonil, fluazinam, fluindapyr, metconazole, prothioconazole, silthiofam, triticonazole, flutianil, fluorofuram, azoxystrobin, difenoconazole, pyridylcarbamate, ipconazole, tetraconazole, silthiofam, dimethomorph, thiophanate-methyl, natamycin, benzene enofluorocarbostyril, fluazinam, triflupyrrole, high efficiency metalaxyl (metamitron), tetraconazole, isoxazoles, bromofluoride, thiamethoxam, imidacloprid, clothianidin, cyantraniliprole, tioxazofen, flufenoxuron and any mixtures thereof.