2812 results about "Blight" patented technology

The invention relates to a strain of lys-starch bacillus as well its application. The bacterium is separated from the soil on eggplant fields, anning district, Lanzhou, Gansu. The bacterium is identified to be Bacillus amyloliquefaciens. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus amyloliquefaciens I7 and preserving code of M206027. The broth of M206027 and M206027strain, non-cell culture fluid of M206027 strain's broth, and the monomer extracted from the M206027strain, M206027 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

A compound agricultural chemical in the form of wettable powder, solution, microemulsion, suspension, water disperser, particle, emulsified oil, or aquatic emulsion for preventing and eliminating diseases and pests of rice, cotton, vegetables, fruit tree, etc, especially the green smut and sheath and culm blight of rice, is proportionally prepared from Bacillus subtitis, hexaconazole, functional assistant, filler and carrier.

The invention discloses a Bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.4992 and application thereof in tomato blight antagonism bacteria. A farmland soil sample on which tomatoes are planted for years in Xinjiang Fukang is subjected to antagonism bacteria screening to obtain a strain with stronger bacteriostatic activity; the strain is further screened and domesticated and bred; and the obtained strain is subjected to morphological specificity and 16S rRNA (Ribose Nucleic Acid) sequencing and system development analysis. The antagonism of the fermentation broth of the strain has outstanding technical effect on solving the tomato blight antagonism bacteria and has wide application field.

The invention relates to a strain of Bacillus subtilis as well its application. The bacterium is separated from the soil on lily fields, fanjiaping, Lanzhou, Gansu. The bacterium is identified to be Bacillus-subtilis. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus-subtilis M22BH3 and preserving code of M206028. The broth of M22BH3 and M22BH3 strain, non-cell culture fluid of M22BH3 strain's broth, and the monomer extracted from the M22BH3 strain, M22BH3 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The invention relates to a biological preventing and controlling strain of continuous cropping cucumber and watermelon blight and microbe organic fertilizer thereof, belonging to agriculture intensification production technologies. Trichoderma harzianum SQR-T037 having obvious biological preventing and controlling effect on cucumber and watermelon blight is separated, and the microbe organic fertilizer is prepared from the biological preventing and controlling strain and organic compost; the fertilizer contains more than 1*108 bacterial colony forming units per gram of the strain, the full nitrogen content is 3-5 percent (more than 90 percent is organic nitrogen), the total NPK nutrient content is 6-10 percent, and the organic matter content is 30-35 percent. An experiment indicates that the microbe organic fertilizer leads the biological preventing and controlling strain to be rapidly bred after being applied to the soil and forms a preponderant population in the soil, and the biological preventing and controlling rate of the blight in the soil having serious cucumber and watermelon blight reaches more than 85 percent. By applying the microbe organic fertilizer to the soil without forming continuous cropping obstruction for a long time, the preventing and controlling rate on the cucumber and watermelon blight reaches more than 80 percent.

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The invention discloses a Bacillius subtilis strain, and the culture preservation code for the Bacillius subtilis at China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.1982. Dry-powder microbial inoculum prepared by the strain can be applied to prevention and cure of wheat scab, rice blast, corn southern leaf blight, sclerotium disease germ of garlic, anthracnose germ of cucumber and other germs that cause diseases to agricultural crops and vegetables; the microbial inoculum can also be applied to preservation of fresh fruits such as peaches, apples, grapes, pears, etc. Rice and corn leaves processed with suspension liquid of Bacillus subtilis strain can prevent the corresponding lesion disease; the cucumber seeds or seedlings processed by mixing the seeds with powder, burying the powder around the root or spraying suspension liquid of Bacillus subtilis strain can not only prevent cucumber anthracnose, but also accelerate the growth of the plants. The production of safe and pollution-free vegetables is capable. Fresh peaches processed with suspension liquid of Bacillus subtilis strain are rot-resistance, and the preservation time is prolonged. The preparation process for the microbial inoculum is simple, applicable to commercial production.

The invention belongs to the technical field of agriculture and discloses a watermelon seedling culturing substrate, which comprises the following components: earthworm cast, vermiculite, pearlstone and paclobutrazol, wherein the volume ratio of the earthworm cast to the vermiculite to the pearlstone is (2-3):0.6:0.4; and the dosage of the paclobutrazol is between 10 g / m<3> and 20 g / m<3>. When the substrate is used for culturing watermelon own-rooted seedlings, a nutrient solution does not need to be replenished, the seedlings are in order and strong without growing excessively, and the root systems are developed. After the substrate is planted into soil along with the seedlings, beneficial microorganisms in the substrate can quickly form a dominant microflora around the rooting zone of a crop, so that nutrition elements in the soil can be activated, and the utilization ratio of a fertilizer is increased; the application amount of an inorganic fertilizer can be reduced by 20-30 percent; soil-borne diseases can be resisted effectively, the incidence rate of field blight is low, and the control effect is between 86.74 percent and 89.4 percent, is higher than the control effect of an efficient bactericide for an own-rooted seedling growing from the ordinary substrate, and is basically close to the control effect of a grafted seedling; and the soluble sugar content of watermelon and the yield are increased.

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The invention relates to a trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and a preparation method thereofof the trichoderma atroviride preparation. A trichoderma strain Tr1208 is trichoderma atroviride and preserved in the CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No. 6828. According to The the trichoderma atroviride preparation takes, the trichoderma atroviride Tr1208 is used as an effective active ingredient and is prepared by being matched with an agriculturally acceptable carrier and a protective agent for the preparation; the bacteria content of the effective active ingredient is (1.0-30.0)*108 spores / gram; and the bacteria protectant content is 0.05%-1.8%. The preparation can have the effects of effectively preventing and controlling leek Botrytis, cucumber brown patches, cucumber powdery mildew, cucumber downy mildew, tomato early blight, cucumber type damping off, cucumber type blight and cucumber type root rot and promoting plant growth and characteristics of efficiency, wide antimicrobial spectrum, simple usage, low cost, no environmental pollution and plant micro ecological environment improvement.

The invention provides a film spreading oiling agent used for preventing and controlling plant diseases and insect pests at a basal part of a rice stem and an application. The film spreading oiling agent comprises insecticidal and sterilization active ingredients and an additive. When the preparation is used, the film spreading oiling agent can be directly poured into a paddy field without dilution by using water. After entering the water, the film spreading oiling agent is distributed and spread as an oil film to spread all over the rice field and climb along a rice straw, and can effectively prevent and control rice stem borer and banded sclerotial blight, thus being a novel pesticide formulation with high working efficiency.

The invention belongs to the field of agricultural chemical bactericides, and discloses an agricultural antifungal composition which is characterized by comprising components A and B, wherein the component A is coumoxystrobin, the component B is a compound selected from triazole bactericides, and the weight ratio of A to B is 1:40-40:1. The composition weakens or retards the generation of resistance of pathogenic fungi to the coumoxystrobin, and avoids the reduction of drug effect resulted from the resistance. Simultaneously, because heterogeneous fungicides form synergism of the composition,the control effect of the composition on diseases is remarkably improved. The composition can be prepared into the formulations of suspending agents, aqueous emulsion, wettable powder or microemulsion, and the like, and is applied to the controlling sclerotinia rot of colza, apple tree canker, rice blast, tomato leaf mold, wheat scab, cucumber scab, tomato anthracnose, cucumber blight, apple ringrot, rice sheath blight, rice bakanae disease, wheat powdery mildew, cucumber powdery mildew, cucumber downy mildew, wheat sheath blight, cucumber gray mold, banana gray pattern disease, and the like.

The re-compounded germicide composition includes kresoxim-methyl and at least one kind of triazole germicide compound in the weight ratio of 0.025-40. The re-compounded germicide composition includes also functional assistant and diluent, and kresoxim-methyl and triazole germicide compound account for 1-95 wt% of the germicide composition. It may be compounded into oil emulsion, soluble liquid preparation, micro emulsion, water emulsion, grain or other forms. It is used in preventing and controlling diseases, such as grape powdery mildew, wheat rust, potato blight, etc. caused by imperfect fungi, ascomycete, basidiomycete and other fungi. It has synergistic effect, wide antifungal spectrum, less resistance and other positive effect.

The invention relates to a method for preparing a composite material which takes modified or unmodified attapulgite as a carrier to immobilize bacillus, belonging to the technical fields of microbial fertilizers, biological control of plant diseases, soil remediation and pollution abatement. A microorganism-mineral immobilization technology is realized by means of preparing a proper amount of attapulgite with a proper amount of bacillus viable bacteria preparation according to a certain technology. The composite material prepared according to the method disclosed by the invention has the advantages that the plant growth can be promoted, the soil-borne diseases, such as root rot, blight, soft rot and the like, of various crops can be inhibited, and the effect of preventing threadworm from spawning is achieved; the composite material further has the capabilities of keeping the local moisture of plant root systems, and enhancing the plant resistance to drought, saline and alkaline as well as oxidization; moreover, the composite material can be further used for purifying organic pollutants in water, absorbing soil heavy metals and relieving soil hardening, so that the effects of soil moisture conservation and fertilizer conservation are achieved. The composite material prepared according to the method disclosed by the invention is an environment-friendly composite material, which has the advantages that environment harm and pollution can be prevented through correct use of the composite material, and a wide application prospect is obtained.

The present invention relates to preparation process of strain and preparation for controlling blight of vegetable biologically. One kind of Pseudomonas chlororaphis GP72 is cultured and screened with sweet pepper rhizoid and its attached soil sample and KMB culture medium with antibiotic added, and preserved in the number of CGMCC No. 1748. The freeze preserved strain is activated and amplifying cultured to obtain microbial preparation, which may be used for preventing and controlling blight of chili, cucumber, wax gourd, etc; for preventing and controlling rape sclerotinia rot, cucumber fusarium wilt, water melon fusarium wilt, etc; and as vegetable growth promoter to raise the yield of vegetable.

The invention relates to the technical field of plant gene engineering, in particular to design, verification and application of a rice disease resistance related DNA molecule of 21nt. An artificial microRNA sequence of the 21nt is artificially designed, and an artificial microRNA precursor is constructed by using a natural microRNAosa-mi528 precursor as a skeleton. In transgenic rice, the artificial microRNA precursor is specifically expressed by using a leaf specific promoter of a rice or arabidopsis thaliana source so that the resistance of the transgenic rice to bacterial leaf blight can be enhanced and important agronomic traits of the transgenic rice such as fertility and the like is not affected at the same time.

The invention discloses a novel bacillus methylotrophicus, which has the preservation register number of CGMCC No.6793, wherein the strain is separated from tobacco rhizosphere soil and is obtained by separating and purifying and screening strains. The invention further provides a fermentation method of the bacillus methylotrophicus, a fermentation product prepared by the method, as well as a biological agent based on the fermentation product and a preparation method thereof; the invention further provides a use of the biological agent in controlling a variety of plant diseases. According to the invention, the biological agent is good in environmental compatibility so that the number of effective living spores is more than 5,000,000,000 / g, has high biological activity, and in particular has good control effect on tobacco bacterial wilt, wherein the control effect on tobacco bacterial wilt, soft rot of cabbage, rice bacterial leaf blight, rice bacterial stripe and cotton angular leaf spot is up to 80% or more.

A composite agricultural chemical for preventing and treating the hypochnus, rotten meck and green smut of paddy rice contains hexaconazole and jianganmycin. Its advantages are high synergistive effect and low by-effect.

The invention relates to a bacillus subtilis preparation for preventing and controlling soil-borne diseases, as well as a preparation method thereof. The bacillus subtilis preparation is prepared by taking the thalli and metabolites of bacillus subtilis B579 with preservation number of CGMCC No.2270 as an effective active constituent which are matched with agriculturally acceptable carrier and a heat-resistant protective agent; the thalli content of the effective active constituent is 8 to 80 billion thalli per gram; and the mass content of the heat-resistant protective agent is 3 to 5 percent. The bacillus subtilis preparation can effectively prevent and control cucumber drooping diseases, cucumber wilt, eggplant root rot, eggplant damping-off, pepper blight, tomato basal stalk rot, grape botrytis cinerea and banana anthracnose. The bacillus subtilis preparation has a control efficiency more than 70 percent, has obvious effect of promoting plant growth, and is characterized in high efficiency, wide antimicrobial spectrum, simple use, low cost, no environmental pollution and improvement on the micro-ecological environment of soil.

The invention discloses a green biological water-flush fertilizer, relating to fertilizers. A manufacturing method is as follows: a, adding animal protein powder, vegetable protein powder, complex bacteria and complex enzyme into an amino acid solution, mixing uniformly, placing the mixture under the temperature of 15-35 DEG C, and after 6-8 days, forming a product A; and b, adding a complex nutrition conditioner, a continuous cropping modifier, a biological insect repellant, a harm removing agent, trace elements, a nitrogen-phosphorus-potassium compound fertilizer into the product A, mixing uniformly and obtaining the green biological water-flush fertilizer. The green biological water-flush fertilizer has the beneficial effects that 1, the sprouting rate can be improved, the fast rootingcan be realized, the sufficient nutrition of plants can be ensured, and premature senility resistance and aging resistance are achieved; 2, the yield and the quality of vegetables, melons and fruits are improved; 3, the utilization ratio, the long-term property, fast effect, long effect and stable efficiency of a base fertilizer are improved; 4, the extremely-good inhibiting effect and improving function are achieved for yellow seedlings, runt seedlings, lobules, leaf roll, blight, pythium debaryanum, droop, dead seedling, rot roots, abnormal rotting disease, virus disease, seedling falling disease and the like; and 5, green and unpolluted foods are beneficial to being produced.

The invention discloses bacillus subtilis S001 capable of inhibiting a variety of pathogens and further discloses a microbial preparation prepared from the bacillus subtilis S001 and a preparation method the microbial preparation. The bacillus subtilis S001 with the collection number of CGMCC No.6425 has the function of promoting growth and inhibiting pathogens including the fusarium, pepper rhizoctonia solani, tomato leaf mold, tomato early blight pathogen, tomato grey mold, eggplant sclerotia pathogen, wheat full-rotting pathogen, wheat root rotting pathogen and wheat sheath blight pathogen. The microbial pesticides and microbial fertilizers prepared from the bacillus subtilis S001 are environmentally-friendly and have good safety, and pathogens can not resist the microbial pesticides and the microbial fertilizers easily.

A chlorothalonil slurry product having greater than 90% by weight of the chlorothalonil present in discrete particles having a diameter less than 1 micron, more preferably less than 0.3 microns, is useful at reduced application rates, compared to prior art chlorothalonil formulations, to control a variety of diseases such as sapstain on wood, neck rot on onions, late blight on potatos, and downey mildew on fruits and vegetables.

A Bacillus subtitis JA with strong supperssion to wheat bakanae fungus is prepared by separating it from soil. Its product is prepared through injecting positive N ions for inducing mutant strains, microbial fermenting to obtain fermented liquid, ultrafiltering, and concentrating. It can be used for preventing and treating more than 9 diseases and pests of agricultural crops, such as wheat bakanae, watermelon blight, etc.

Provided is a light emitting device with improved light extracting efficiency and further higher heat releasing performance. A light emitting device includes a planar lead frame having a first lead and a second lead, and includes a light emitting element mounted on the first lead, a resin frame surrounding a periphery of the light emitting element, a first sealing resin filled in the inner side of the resin frame and sealing the light emitting element, and a second sealing resin covering the resin frame and the first sealing resin. Lower end of inner surface of the resin frame is arranged only on the first lead, and at an outside of the resin frame, and the second resin member covers at least a part of the first lead and the second lead. Of the back-surface of the first lead, a region directly under the blight emitting element is exposed.

The present invention is recompounded microbe germicide for preventing and controlling banded sclerotial blight and green smut of rice. The recompounded microbe germicide is recompounded through thefermentation culture of Bacillus subtilis and recompounding the fermented liquid with certain content of Bacillus subtilis and Jinggangmeisu. It is used in preventing and controlling banded sclerotial blight and green smut of rice in field via utilizing the complementary superiority of Bacillus subtilis and Jinggangmeisu. It has high functions of preventing and controlling banded sclerotial blight and green smut of rice, less influence on rice quality, stable germicidal effect and long preservation period.

The invention relates to the fields of gene sequences and acquiring methods thereof, and concretely relates to SNP loci linked with a blight resistant gene Fon-1 in a watermelon, markers thereof, and acquiring methods of the loci and the markers. The SNP loci linked with a blight resistant gene Fon-1 in a watermelon and the markers thereof have nucleotide base sequences represented by SEQ ID NO:1-8 in a sequence table. The acquiring method of the loci comprises the following steps: 1, selecting a tested material; 2, carrying out preliminary positioning of the blight resistant gene Fon-1 in the watermelon; 3, acquiring candidate SNP; and 4, acquiring the SNP loci closely linked with the blight resistant gene Fon-1 in the watermelon, and verifying the candidate SNP loci. According to the invention, the phenotype identification and the molecular detection of F2 generation separation populations and natural populations are carried out through utilizing a series of markers to confirm the close linkage degree of the series of the SNP loci with target properties; and the SNP loci are utilized to design the markers respectively, and the markers can be utilized to carry out the initial-stage screening of the kind in order to reach a molecule assisted breeding purpose, so the breeding period is substantially shortened, and an important scientific research meaning is possessed.

The invention belongs to microbial fermentation, and particularly relates to a preparation method of a probiotic inoculum for preventing replant disease of greenhouse cucumbers. The preparation method comprises a fermentation step: inoculating a probiotic inoculum producing strain into a sterile culture medium containing a carbon source, a nitrogen source, inorganic salt and water; carrying out aerobic fermentation under the conditions that the temperature is 30-37 DEG C and the pH value is 7.2-7.6 in the fermentation process, wherein the producing strain is a mixture of Bacillus subtilis, Bacillus licheniformis, Trichosporon behrendii, Paecilomyces lilacinus, Streptomyces microflavus, Bacillus polymyxa and Metarhizium anisopliae of the same mass; and adsorbing the final fermentation product, and immobilizing on grass carbon to obtain the probiotic inoculum. The preparation method solves the defects of replant disease, root rot, blight, nematode disease and the like in the existing greenhouse vegetable-cucumber planting technology, has the advantages of low production cost, short fermentation period and high flora density, and can effectively prevent seedling death, seedling yellowing, blight, mosaic disease, root rot and other ecological symptoms of soil. Meanwhile, the preparation method can promote the growth of roots, loosen the soil, strengthen the seedlings and degrade the residual pesticides and fertilizers, and has an effect of obviously restoring the soil ecosystem. By using the preparation method, the yield of greenhouse cucumbers can be increased by 15-20% in one season.

The invention belongs to the technical field of biological pesticides, discloses a Streptomyces l avendulae xjy strain which has been collected in the general microorganism center of CCCCM in 8.16.2007 with the collection number: CCMCC No.2130. The invention also discloses a separation and authentication method for the strain, a preparation method for antibiotic active substances of the Streptomyces l avendulae xjy strain, authentication for analysis structures of the active substances, prevention and cure experiments for metabolic active substances of the xjy strain against leaf muld of tomato, powdery mildew of cucumber and phytophthora blight of pepper, and growth-promoting experiments for the growth of the strain. The antibiotic active substances secreted by Streptomyces xjy strain is mainly nucleosides antibiotic having the characteristics of persistent effect and no medicinal hazard as well as having the effects of obviously preventing and curing vegetable diseases and promoting the growth to a certain extent.