354 results about "Blight" patented technology

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The inventive technique process of grainy calcium material for preventing and treating peanut blight primarily includes: adopting the shell powder and weathered coal as raw materials, machining into peanut blight prevention material through agglomeration granulation. The invention has innovative and reasonable design, which not only has simple process, easy implementing, but also has low cost of raw materials, product application convenience, remarkable peanut blight prevention effect, and capability of promoting high-efficient production pf peanut. In particular, the invention applies coastal oyster shell wastes for machining calcium materials for preventing and treating peanut blight, which not only reduces environmental pollution, but also promotes recycling of resources, has very significant economic, social and ecological benefits and greater promotional value.

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention belongs to the technical field of biological control, and particularly relates to a pseudomonas flax DY-31 strain, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the pseudomonas lini (Pseudomonas lini) DY-31 is CGMCC (China General Microbiological Culture Collection Center) No.34088. The pseudomonas lini (Pseudomonas lini) DY-31 is The pseudomonas flax DY-31 or the biocontrol inoculant thereof is applied to prevention and treatment of plant soil-borne diseases, specifically including peanut black rot, peanut southern blight, peanut sclerotinia rot, peanut leaf rot sclerotinia rot, peanut basal rot and wheat stem basal rot. The pseudomonas flax DY-31 provided by the invention is an important strain resource, can be used for creating a biopesticide preparation, and is used for green prevention and treatment of soil-borne diseases of peanuts and wheat. In addition, the DY-31 strain also has various growth promoting functions, and has important development and application values in the aspects of agricultural biological control and plant growth promoting.

The invention discloses pseudomonas chlororaphis S7-24 for antagonizing xanthomonas oryzae and application of the pseudomonas chlororaphis S7-24, and belongs to the technical field of microorganisms. The pseudomonas chlororaphis S7-24 is preserved in the China Center for Type Culture Collection on November 12, 2025, and the preservation number of the pseudomonas chlororaphis S7-24 is CCTCC NO: M 20252533. The pseudomonas chlororaphis S7-24 provided by the invention is a newly found pseudomonas biocontrol resource, and verification shows that the strain S7-24 has relatively high antagonistic activity on rice bacterial blight and rice bacterial streak, and can be used for effectively preventing and treating rice bacterial blight and rice bacterial streak. The pseudomonas chlororaphis S7-24 provided by the invention is used for preventing and treating rice bacterial leaf blight and rice bacterial leaf streak, can reduce the use amount of chemical pesticides, ensures grain safety, and has better economic and ecological benefits.

The application discloses an NLR plug-in gene for improving plant disease resistance and application thereof, and belongs to the field of plant molecular biology and biotechnology.The NLR plug-in gene StEM1 has a whole gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO.3.The gene plays a key role in improving plant disease resistance, especially resistance to field late blight variant bacteria.In the NLR background, overexpression of the gene significantly promotes the disease resistance of tobacco and potato to pathogenic pythium, and the gene is a gene for enhancing plant disease resistance by improving ETI immunity.Overexpression of the gene in the NLR background is obtained by a genetic transformation method, and a strain line is obtained to resist field variant strains, so as to cope with field diseases of crops.The application provides an excellent gene resource for green prevention and control of late blight and disease-resistant genetic engineering breeding.

The invention discloses marine-derived penicillium as well as a fermentation method and application thereof. The marine source penicillium is named as Penicillium sp.DSF059, and the preservation number of the marine source penicillium is CCTCC (China Center For Type Culture Collection) NO: M 20232310. According to the invention, the culture conditions of the strain are optimized, the culture medium and culture conditions with high yield of antibacterial active compounds are selected for fermentation, more target products can be obtained, the operation is simple, the cost is low, and the method is suitable for industrial production. Meanwhile, the marine-derived penicillium sp. DSF059 can provide an excellent strain for the development of new drugs for resisting plant pathogens such as coffee leaf blight, coffee brown spot, grape gray mold, golden cypress brown spot, citrus black rot, phytophthora capsici, hickory leaf blight and coffee black spot as well as four pathogens separated from coffee leaf diseases and staphylococcus aureus; good application prospects are realized on prevention and treatment of phytopathogen and staphylococcus aureus.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a biological product for overexpressing an OsARF7 gene to improvement of rice disease resistance, the nucleotide sequence of the OsARF7 gene is shown as SEQ ID NO.1, and the amino acid sequence of the OsARF7 gene is shown as SEQ ID NO.2. According to the invention, an OsARF7 overexpression rice strain is constructed by means of transgenosis. Tests show that the overexpression of the OsARF7 can significantly enhance the immunocompetence of the rice, for example, the expression level of a disease-resistant related gene is improved, active oxygen outbreak is significantly induced, and the resistance of the rice to false smut and bacterial leaf blight is significantly improved. The invention proves that the OsARF7 has a positive regulation effect on rice immunity, can be used for improving the disease resistance of rice, and has important significance for creating disease-resistant rice germplasm resources.

The invention relates to the technical field of biology, in particular to application of an OgRKa gene and an upstream promoter thereof to improvement of rice bacterial blight and rice blast resistance, nucleotide sequences of the OgRKa gene and the upstream promoter are as shown in SEQ ID No.1, a CDS sequence is as shown in SEQ ID No.4, and an amino acid sequence of an encoding protein OgRKa is as shown in SEQ ID No.5. The homologous gene OgRKa of the rice MRKa gene (a rice plant over-expressing the MRKa gene has partial resistance to the rice bacterial blight, and a rice plant expressing the MRKa gene by using an MRKa self promoter has no resistance to the rice bacterial blight) is homologous cloned from oryza meyeriana through PCR (Polymerase Chain Reaction) amplification. Compared with the prior art, the resistance of rice plants with the OgRKa gene overexpressed by the 35S promoter or the OgRKa gene expressed by the promoter per se to a plurality of xanthomonas oryzae and magnaporthe oryzae is remarkably enhanced, the approximate immune level is achieved, the broad-spectrum high-resistance xanthomonas oryzae and magnaporthe oryzae are shown, and good application prospects are achieved in the aspect of breeding of xanthomonas oryzae and magnaporthe oryzae resistant varieties.

The invention discloses bacillus tequilensis 83IBC04-2 and application thereof, the bacillus tequilensis 83IBC04-2 comes from a deep sea hydrothermal zone and is preserved in China Center for Type Culture Collection on October 15, 2025, and the preservation number is CCTCC NO: M20252212. The compound has a remarkable antagonistic effect on a plurality of important pathogenic fungi such as tomato gray mold, tomato early blight, rice blast and wheat scab, can be used for preparing a broad-spectrum and high-efficiency biological bactericide, effectively reduces the use amount of chemical pesticides, and reduces pesticide residues and environmental pollution.

The invention relates to the technical field of agricultural disease detection and crop disease resistance screening, in particular to a rice bacterial leaf blight disease resistance screening method based on an unmanned aerial vehicle and deep learning, and adopts the technical scheme that an optimized YOLOv11-OBB model is composed of a C3k2FC module, an SPPFLSKA module, a SlimNeck module and a LiteHead module, is responsible for extracting features in an image and positioning bacterial leaf blight spots, and is used for screening the disease resistance of the rice bacterial leaf blight; bacterial leaf blight can be efficiently and accurately detected; a lightweight deep learning model is used, efficient operation on a resource-limited unmanned aerial vehicle platform can be realized, and the equipment and calculation cost is reduced; through the cooperation of a plurality of optimization modules, the detection precision of bacterial leaf blight spots is remarkably improved, and a stable detection effect can be kept; the operation process is simplified, and full automation of bacterial blight disease resistance screening is realized; therefore, low-cost, efficient, automatic and high-precision bacterial blight disease resistance screening is realized by utilizing the lightweight and optimized YOLOv11-OBB model and combining module design with high calculation efficiency, and the method is suitable for large-scale field application.

The invention relates to the technical field of prevention and treatment of plant diseases, in particular to application of a biocontrol strain hzq1601 to prevention and treatment of rice bacterial blight. The biocontrol strain hzq1601 is a bacterial strain hzq1601 of bacillus amyloliquefaciens, the preservation number of the bacterial strain hzq1601 is GDMCC (China General Microbiological Culture Collection Center) NO: 60355, and the biocontrol strain hzq1601 is a bacterial strain hzq1601 of bacillus amyloliquefaciens with the preservation number of GDMCC NO: 60355. The invention further provides a method for preventing and treating the rice bacterial blight, in the rice seedling stage, the biocontrol strain hzq1601 bacterial liquid is sprayed to leaves, so that the morbidity of the rice bacterial blight is remarkably reduced to 1% or below, and losses caused by the bacterial blight are reduced; the bacterial liquid of the biocontrol strain hzq1601 is sprayed to the surface of soil in the tillering stage or booting stage, and the bacterial leaf blight prevention effect and the disease index prevention effect of the biocontrol strain hzq1601 are obviously higher than those of other treatments under the condition that the dosage of the bacterial liquid for seedling treatment is small. The action path of the bacillus amyloliquefaciens is direct and efficient, especially, the strong control effect can be achieved through low-dose spraying in the seedling stage, cost and operation difficulty are reduced, a stable pesticide application window and strategy are formed, and wide popularization prospects are achieved.

The invention provides a stenotrophomonas sp. CLX-022 strain which has a good antagonistic effect on colletotrichum gloeosporioides and fusarium fusiforme, and the strain is preserved in China Center for Type Culture Collection (address: Wuhan University, Wuhan, China) and has a good antagonistic effect on colletotrichum gloeosporioides and fusarium fusiforme. The strain is preserved in China Center for Type Culture Collection (CCTCC) NO: M20252110 on September 24, 2025, the postal code is 430072, and the preservation number is CCTCC NO: M20252110. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The stenotrophomonas CLX-022 strain is high in antibacterial property and can be used for preparing a microbial preparation for treating / preventing strawberry anthracnose and rice bakanae disease.

The invention relates to a bacillus velezensis strain B12, a fungicide containing the same and application of the bacillus velezensis strain B12. The bacillus velezensis strain B12 disclosed by the invention has an efficient and lasting antagonistic effect on septospora (especially tomato septospora), and can play a key role in biological prevention and control of tomato spot blight. Furthermore, research on endogenous metabolites shows that the volatile metabolite 4-hydroxycoumarin can remarkably improve the effects of antagonizing the tomato Acetrospora chrysosporium and preventing and treating tomato spot blight, so that the 4-hydroxycoumarin can be used for developing a more efficient compound bacterial agent. The bacterial strain and the compound bacterial agent have the characteristics of high efficiency, durability, safety and environmental protection when being used for antagonizing septospora caninum and preventing and treating tomato spot blight caused by septospora caninum, so that the bacterial strain and the compound bacterial agent have relatively high industrial value and application prospect.

The invention discloses Erwinia phyllostachys ZX-13 and application thereof in antagonism of epidemic disease pathogenic bacteria of Eupin, and belongs to the technical field of microorganisms. The invention discloses a Erwinia phyllostachys ZX-13, and the preservation number of the Erwinia phyllostachys ZX-13 is GDMCC No: 66800. The Erwinia phyllostachys ZX-13 disclosed by the invention can be used for preventing and treating the epidemic disease of the elaphus japonicus caused by the cryptophyton pseudophyllanthus of the elaphus japonicus; the Erwinia rhizosphere ZX-13 is a biological control potential strain with good antagonism effect, high control effect and good environmental safety, and has good development and application prospects.

The invention discloses an application of a rice bHLH transcription factor OsbHLH024 and a coding gene thereof in disease-resistant breeding, and belongs to the technical field of gene engineering. The amino acid sequence of the rice bHLH transcription factor OsbHLH024 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the gene OsbHLH024 is as shown in SEQ ID NO. 1. Experiments prove that through overexpression of the OsbHLH024 gene in the Zhonghua 11, the resistance of rice to rice blast, banded sclerotial blight and leaf blight can be remarkably improved; the OsbHLH024 gene is knocked out, and the resistance of rice to rice blast, banded sclerotial blight and leaf blight can be remarkably reduced. Therefore, the OsbHLH024 gene can positively regulate the disease resistance of the plant, which is of great significance to the cultivation of disease-resistant rice varieties.

The invention belongs to the technical field of gene engineering, and discloses application of an F-box protein gene OsFBX235 in improvement of bacterial blight resistance. The invention specifically discloses an application of knocking out OsFBX235 gene in improving the bacterial blight resistance of rice or cultivating a bacterial blight resistant rice variety. The F-box protein coding gene OsFBX235 is separated and cloned from rice, and the gene is proved to participate in the defensive reaction of the rice to the bacterial blight for the first time through functional analysis, and is an important negative regulation factor for regulating the resistance of the rice bacterial blight. The gene OsFBX235 is knocked out through target gene screening in combination with a CRISPR / Cas9 technology, and the importance of the gene OsFBX235 on resistance regulation and control of the rice bacterial blight is proved. Meanwhile, knockout of the gene can be used for creating rice germplasm resources with high bacterial leaf blight resistance and can be applied to breeding of new varieties with the bacterial leaf blight resistance of the rice.

The application discloses a marine source penicillium and a fermentation method and application thereof. The marine source penicillium is named Penicillium sp. DSF059, and the preservation number is CCTCC NO: M 20232310. Penicilliu The application optimizes the culture condition of the strain, selects a culture medium and culture condition with high yield of antibacterial active compounds to carry out fermentation, so that more target products can be obtained, the operation is simple, the cost is low, and meanwhile, the marine source penicillium Penicilliu Penicillium sp. DSF059 can provide an excellent strain for new drug development of resisting plant pathogenic fungi such as coffee leaf blight, coffee brown spot disease, grape gray mold disease, Chinese cinnamon brown spot disease, citrus black rot disease, pepper late blight, hickory leaf blight, coffee black spot disease and four pathogenic fungi separated from coffee leaf diseases and Staphylococcus aureus, and has a good application prospect for preventing and treating the plant pathogenic fungi and Staphylococcus aureus.

The invention discloses a method for cultivating a heat-resistant broad-spectrum durable rice blast-resistant rice breeding material based on polygene polymerization. According to the method, a high-heat-resistance and high-dominance indica-japonica hybrid indica rice restorer line is selected as a recurrent parent, a breeding intermediate material for polymerizing complementary broad-spectrum rice blast resistance genes Pigm and Pi-jx is used as a donor parent, and polymerization of the heat-resistance and broad-spectrum lasting rice blast resistance genes Pigm and Pi-jx is realized through a method of combining molecular breeding with conventional breeding. Meanwhile, by selecting representative strains to carry out artificial inoculation identification of seedling blast and panicle blast, natural induction identification of a rice blast retransmission area and heat resistance identification, and by combining main agronomic trait evaluation in the whole growth period, a heat-resistant broad-spectrum durable rice blast-resistant breeding material is obtained. The breeding material bred by using the method disclosed by the invention has relatively strong heat resistance and broad-spectrum and lasting rice blast resistance, and the breeding process of heat-resistant and rice blast-resistant rice varieties is accelerated.

The invention belongs to the field of molecular biology, and particularly relates to application of an OsNiR1 gene to improvement of rice broad-spectrum disease resistance and improvement of rice agronomic traits. The invention provides an application of an OsNiR1 gene, and the application comprises an application in preventing and treating plant diseases and / or enhancing the resistance of plants to pathogenic bacteria, and / or an application in improving agronomic traits of the plants. The amino acid sequence coded by the OsNiR1 gene is as shown in SEQ ID NO. 1, and the CDS sequence of the OsNiR1 gene is as shown in SEQ ID NO. 2. The application proves that OsNiR1 participates in regulating and controlling the immune response pathway of rice to fungal disease rice blast, fungal disease banded sclerotial blight and bacterial disease bacterial leaf blight. Meanwhile, after the strong promoter ubi is adopted for driving, an overexpression material of the OsNiR1 is higher and stronger than a wild plant type, and the tiller number is increased. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The invention discloses a potato late blight antagonistic bacterium and application thereof, belongs to biological agents, and provides more strain resources for prevention and treatment of potato late blight. The potato late blight antagonistic bacterium is Pseudomonas chlororaphis blT-49 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 27, 2021, and the preservation number is CGMCC No.24203. The invention further discloses a preparation method of the potato late blight antagonistic bacterium. The strain disclosed by the invention not only can inhibit the occurrence and growth of potato late blight, but also can regulate and control the ecological system of the micro-ecology of potatoes, reduce the infection ability of potato late blight and improve the disease resistance of potatoes. In the growth and development of the potatoes, the growth and development of the potatoes can be promoted, and the single-plant yield is improved.

The application discloses application of BoDMR1 and BoDMR6 genes in cultivating disease-resistant broccoli varieties, an expression vector and a cultivating method, and relates to the technical field of plant molecular biology. A nucleotide sequence of a broccoli BoDMR1 gene is shown in SEQ ID No: 7, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 7; and a nucleotide sequence of a broccoli BoDMR6 gene is shown in SEQ ID No: 8, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 8. Through gene editing on endogenous BoDMR1 and / or BoDMR6 genes of plants, mutant plants showing resistance to diseases such as sclerotinia, black rot and alternaria blight can be obtained. Therefore, the application is beneficial to providing good resources for cultivating disease-resistant broccoli varieties.

The invention discloses application of m-cresol to prevention and treatment of pepper southern blight, and belongs to the field of plant disease prevention and treatment. According to the invention, it is found for the first time that m-cresol has an inhibition effect on sclerotium rolfsii, wherein EC50 of m-cresol is 0.1560 [mu] L / mL; the m-cresol with the concentration of 2 * EC50 can completely inhibit sclerotium germination, sclerotium generation and hypha growth of the sclerotium rolfsii. Furthermore, m-cresol can be used for preventing and treating pepper southern blight caused by sclerotium rolfsii, the morbidity and disease index of pepper southern blight can be effectively reduced by using a m-cresol solution with the concentration of 0.125% to perform root irrigation treatment on pepper planted in pathogenic bacteria soil, the relative prevention and treatment effect reaches 100%, and adverse reaction of pepper plants cannot be caused. The invention provides a new method for controlling the pepper southern blight by using a medicament, and the method has the advantages of high efficiency, low cost, low environmental harm and the like.

The invention relates to a dispersible oil suspending agent for preventing and treating potato late blight and a preparation method and application thereof, and relates to the field of pesticides, the dispersible oil suspending agent comprises the following raw materials by weight: 5-25% of mandipropamid, 5-25% of cymoxanil, 5-20% of a dispersant, 5-20% of an emulsifier, 2-5% of sodium carboxymethyl cellulose, and 27-65% of solvent oil 200 #. Field control experiments show that the dispersible oil suspending agent for preventing and treating potato late blight can effectively prevent and treat potato late blight, is safe and remarkable in prevention and treatment effect, and has no adverse effect on other diseases and insect pests and no adverse effect on non-target organisms.

This invention discloses an oxyindene compound containing substituted pyridine and its application as a fungicide. The oxyindene compound containing substituted pyridine has broad-spectrum fungicidal activity, especially effective in controlling rice sheath blight, apple tree rot, rapeseed sclerotinia stem rot, wheat scab, pepper blight, cucumber anthracnose, tomato gray mold, corn curvature blight, rice blast, tomato early blight, wheat take-all, and wheat stem rot. It can be used as a fungicide for the prevention and control of the above-mentioned plant diseases.

The invention belongs to the technical field of phytopathogen detection, and particularly relates to a specific detection primer and a specific detection kit for Cercospora cf.flagellaris, and an application of the specific detection primer and the specific detection kit for Cercospora cf.flagellaris. By adopting the detection primer or the kit disclosed by the invention, the specific detection of the phytolacca acinosa can be realized, and the detection primer or the kit has good intraspecific strain universality. Meanwhile, through a sensitivity experiment, the detection system and the detection method established by the invention have the lowest detection concentration of 0.012 pg.L <-1 > aiming at the phytolacca acinosa. The detection system is used for detecting soybean grains with purple spot symptoms in the field, and can be used for quickly and accurately identifying whether soybean plants carry pathogenic bacteria of sporospora phytolacca or not. Therefore, the detection primer and the kit provided by the invention have good application prospects.

The invention discloses a transcription factor AcWRKY84 gene for regulating and controlling gray mold resistance of kiwi fruits. After the expression of the gene is silenced, the gray mold resistance of kiwi fruits can be remarkably reduced. The AcWRKY84 gene is cloned, and the anti-gray mold effect of the AcWRKY84 gene in kiwi fruits is proved, specifically, after an experimental group is inoculated with viruses, compared with a control group, the disease symptoms of the fruits of the experimental group are aggravated, the infected area is obviously enlarged, and the enzyme activities of SOD, POD and CAT in the fruits are obviously reduced compared with the control group; sufficient gene resources can be provided for a kiwi fruit gray mold resistance gene pool, so that the possibility of successfully breeding high-gray mold-resistance varieties subsequently is greatly increased.

The invention discloses a method and a system for detecting rice bacterial leaf blight based on unmanned aerial vehicle spatial spectrum fusion. The method comprises the following steps: firstly, constructing a rice bacterial leaf blight detection data set; a rice bacterial leaf blight severity grading detection double-branch model is constructed, and the model adopts a spectrum-space double-branch parallel coding and fusion framework and comprises a spectrum branch module, a space branch module and a cross attention fusion mechanism module. And inputting the complete image of the rice field into the trained rice bacterial leaf blight severity grading detection double-branch model, and obtaining severity grading detection results of the bacterial leaf blight in different rice growth periods of the target field. According to the invention, deep complementation and dynamic information balance of spectrum and spatial features of the multispectral unmanned aerial vehicle are realized, so that the accuracy of disease detection and the generalization ability of the model are effectively improved.