1305 results about "Blight" patented technology

The invention relates to a strain of lys-starch bacillus as well its application. The bacterium is separated from the soil on eggplant fields, anning district, Lanzhou, Gansu. The bacterium is identified to be Bacillus amyloliquefaciens. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus amyloliquefaciens I7 and preserving code of M206027. The broth of M206027 and M206027strain, non-cell culture fluid of M206027 strain's broth, and the monomer extracted from the M206027strain, M206027 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The invention belongs to the technical field of agriculture and discloses a watermelon seedling culturing substrate, which comprises the following components: earthworm cast, vermiculite, pearlstone and paclobutrazol, wherein the volume ratio of the earthworm cast to the vermiculite to the pearlstone is (2-3):0.6:0.4; and the dosage of the paclobutrazol is between 10 g/m<3> and 20 g/m<3>. When the substrate is used for culturing watermelon own-rooted seedlings, a nutrient solution does not need to be replenished, the seedlings are in order and strong without growing excessively, and the root systems are developed. After the substrate is planted into soil along with the seedlings, beneficial microorganisms in the substrate can quickly form a dominant microflora around the rooting zone of a crop, so that nutrition elements in the soil can be activated, and the utilization ratio of a fertilizer is increased; the application amount of an inorganic fertilizer can be reduced by 20-30 percent; soil-borne diseases can be resisted effectively, the incidence rate of field blight is low, and the control effect is between 86.74 percent and 89.4 percent, is higher than the control effect of an efficient bactericide for an own-rooted seedling growing from the ordinary substrate, and is basically close to the control effect of a grafted seedling; and the soluble sugar content of watermelon and the yield are increased.

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The invention provides a film spreading oiling agent used for preventing and controlling plant diseases and insect pests at a basal part of a rice stem and an application. The film spreading oiling agent comprises insecticidal and sterilization active ingredients and an additive. When the preparation is used, the film spreading oiling agent can be directly poured into a paddy field without dilution by using water. After entering the water, the film spreading oiling agent is distributed and spread as an oil film to spread all over the rice field and climb along a rice straw, and can effectively prevent and control rice stem borer and banded sclerotial blight, thus being a novel pesticide formulation with high working efficiency.

The re-compounded germicide composition includes kresoxim-methyl and at least one kind of triazole germicide compound in the weight ratio of 0.025-40. The re-compounded germicide composition includes also functional assistant and diluent, and kresoxim-methyl and triazole germicide compound account for 1-95 wt% of the germicide composition. It may be compounded into oil emulsion, soluble liquid preparation, micro emulsion, water emulsion, grain or other forms. It is used in preventing and controlling diseases, such as grape powdery mildew, wheat rust, potato blight, etc. caused by imperfect fungi, ascomycete, basidiomycete and other fungi. It has synergistic effect, wide antifungal spectrum, less resistance and other positive effect.

The invention relates to the technical field of plant gene engineering, in particular to design, verification and application of a rice disease resistance related DNA molecule of 21nt. An artificial microRNA sequence of the 21nt is artificially designed, and an artificial microRNA precursor is constructed by using a natural microRNAosa-mi528 precursor as a skeleton. In transgenic rice, the artificial microRNA precursor is specifically expressed by using a leaf specific promoter of a rice or arabidopsis thaliana source so that the resistance of the transgenic rice to bacterial leaf blight can be enhanced and important agronomic traits of the transgenic rice such as fertility and the like is not affected at the same time.

The invention discloses a novel bacillus methylotrophicus, which has the preservation register number of CGMCC No.6793, wherein the strain is separated from tobacco rhizosphere soil and is obtained by separating and purifying and screening strains. The invention further provides a fermentation method of the bacillus methylotrophicus, a fermentation product prepared by the method, as well as a biological agent based on the fermentation product and a preparation method thereof; the invention further provides a use of the biological agent in controlling a variety of plant diseases. According to the invention, the biological agent is good in environmental compatibility so that the number of effective living spores is more than 5,000,000,000/g, has high biological activity, and in particular has good control effect on tobacco bacterial wilt, wherein the control effect on tobacco bacterial wilt, soft rot of cabbage, rice bacterial leaf blight, rice bacterial stripe and cotton angular leaf spot is up to 80% or more.

The invention relates to a bacillus subtilis preparation for preventing and controlling soil-borne diseases, as well as a preparation method thereof. The bacillus subtilis preparation is prepared by taking the thalli and metabolites of bacillus subtilis B579 with preservation number of CGMCC No.2270 as an effective active constituent which are matched with agriculturally acceptable carrier and a heat-resistant protective agent; the thalli content of the effective active constituent is 8 to 80 billion thalli per gram; and the mass content of the heat-resistant protective agent is 3 to 5 percent. The bacillus subtilis preparation can effectively prevent and control cucumber drooping diseases, cucumber wilt, eggplant root rot, eggplant damping-off, pepper blight, tomato basal stalk rot, grape botrytis cinerea and banana anthracnose. The bacillus subtilis preparation has a control efficiency more than 70 percent, has obvious effect of promoting plant growth, and is characterized in high efficiency, wide antimicrobial spectrum, simple use, low cost, no environmental pollution and improvement on the micro-ecological environment of soil.

The invention discloses a green biological water-flush fertilizer, relating to fertilizers. A manufacturing method is as follows: a, adding animal protein powder, vegetable protein powder, complex bacteria and complex enzyme into an amino acid solution, mixing uniformly, placing the mixture under the temperature of 15-35 DEG C, and after 6-8 days, forming a product A; and b, adding a complex nutrition conditioner, a continuous cropping modifier, a biological insect repellant, a harm removing agent, trace elements, a nitrogen-phosphorus-potassium compound fertilizer into the product A, mixing uniformly and obtaining the green biological water-flush fertilizer. The green biological water-flush fertilizer has the beneficial effects that 1, the sprouting rate can be improved, the fast rootingcan be realized, the sufficient nutrition of plants can be ensured, and premature senility resistance and aging resistance are achieved; 2, the yield and the quality of vegetables, melons and fruits are improved; 3, the utilization ratio, the long-term property, fast effect, long effect and stable efficiency of a base fertilizer are improved; 4, the extremely-good inhibiting effect and improving function are achieved for yellow seedlings, runt seedlings, lobules, leaf roll, blight, pythium debaryanum, droop, dead seedling, rot roots, abnormal rotting disease, virus disease, seedling falling disease and the like; and 5, green and unpolluted foods are beneficial to being produced.

A chlorothalonil slurry product having greater than 90% by weight of the chlorothalonil present in discrete particles having a diameter less than 1 micron, more preferably less than 0.3 microns, is useful at reduced application rates, compared to prior art chlorothalonil formulations, to control a variety of diseases such as sapstain on wood, neck rot on onions, late blight on potatos, and downey mildew on fruits and vegetables.

A Bacillus subtitis JA with strong supperssion to wheat bakanae fungus is prepared by separating it from soil. Its product is prepared through injecting positive N ions for inducing mutant strains, microbial fermenting to obtain fermented liquid, ultrafiltering, and concentrating. It can be used for preventing and treating more than 9 diseases and pests of agricultural crops, such as wheat bakanae, watermelon blight, etc.

Provided is a light emitting device with improved light extracting efficiency and further higher heat releasing performance. A light emitting device includes a planar lead frame having a first lead and a second lead, and includes a light emitting element mounted on the first lead, a resin frame surrounding a periphery of the light emitting element, a first sealing resin filled in the inner side of the resin frame and sealing the light emitting element, and a second sealing resin covering the resin frame and the first sealing resin. Lower end of inner surface of the resin frame is arranged only on the first lead, and at an outside of the resin frame, and the second resin member covers at least a part of the first lead and the second lead. Of the back-surface of the first lead, a region directly under the blight emitting element is exposed.

The invention relates to the fields of gene sequences and acquiring methods thereof, and concretely relates to SNP loci linked with a blight resistant gene Fon-1 in a watermelon, markers thereof, and acquiring methods of the loci and the markers. The SNP loci linked with a blight resistant gene Fon-1 in a watermelon and the markers thereof have nucleotide base sequences represented by SEQ ID NO:1-8 in a sequence table. The acquiring method of the loci comprises the following steps: 1, selecting a tested material; 2, carrying out preliminary positioning of the blight resistant gene Fon-1 in the watermelon; 3, acquiring candidate SNP; and 4, acquiring the SNP loci closely linked with the blight resistant gene Fon-1 in the watermelon, and verifying the candidate SNP loci. According to the invention, the phenotype identification and the molecular detection of F2 generation separation populations and natural populations are carried out through utilizing a series of markers to confirm the close linkage degree of the series of the SNP loci with target properties; and the SNP loci are utilized to design the markers respectively, and the markers can be utilized to carry out the initial-stage screening of the kind in order to reach a molecule assisted breeding purpose, so the breeding period is substantially shortened, and an important scientific research meaning is possessed.

The invention belongs to the technical field of biological pesticides, discloses a Streptomyces l avendulae xjy strain which has been collected in the general microorganism center of CCCCM in 8.16.2007 with the collection number: CCMCC No.2130. The invention also discloses a separation and authentication method for the strain, a preparation method for antibiotic active substances of the Streptomyces l avendulae xjy strain, authentication for analysis structures of the active substances, prevention and cure experiments for metabolic active substances of the xjy strain against leaf muld of tomato, powdery mildew of cucumber and phytophthora blight of pepper, and growth-promoting experiments for the growth of the strain. The antibiotic active substances secreted by Streptomyces xjy strain is mainly nucleosides antibiotic having the characteristics of persistent effect and no medicinal hazard as well as having the effects of obviously preventing and curing vegetable diseases and promoting the growth to a certain extent.

The invention discloses a pseudomonas aeruginosa D10 which is preserved in China Typical Culture Preservation Center, and the preservation number is CCTCC NO: M 209104. A colony on a beef paste peptone culture medium appears brown, round, glossy and wet, and has trim edges; the diameter of the colony is 0.2-0.5 cm; and the thallus is in a short bar shape and does not have gemma. The invention also discloses a preparation method and applications of the pseudomonas aeruginosa D10. The pseudomonas aeruginosa D10 of the invention can inhibit corn northern and southern leaf blight, has strong antergic functions on corn northern leaf blight, corn southern leaf blight, corn curvalaria leaf spot, cotton verticillium wilt, wheat fusarium graminearum, banana colletotrichum toilette and banana fusarium wilt, has the advantages of wide bacteriostatic range, high prevention efficiency and favorable environmental safety, and has favorable prospects for development and applications.

The invention provides a nutrient fertilizer for fuchsia. The nutrient fertilizer can meet the basic nutrient requirements of the whole growth period of the fuchsia, and is favorable for promoting growth and development of roots and leaves of the fuchsia. The fertilizer has sterilization, pest killing and pest and disease prevention while being applied, and has a good effect of preventing, controlling or relieving aphids, blight, rust disease, root rot, powdery mildew and the like possibly produced in the fuchsia. After the fertilizer is applied, the fuchsia has the advantages of large and multiple flower buds, bright color, few pest and disease and quick growth and development speed and also has high ornamental value.

The invention discloses a planting method for regulating and controlling continuous cropping obstacles of atractylides macrocephala koidz, which comprising the following steps of: applying 40 to 100g of lime nitrogen to each square meter of a continuous cropping field, performing deep tillage, watering soil by using 500 to 1,000 times of prochloraz or fluazinam, covering by using a thin film for 15 to 20 days, uncovering the thin film, ventilating for 10 to 15 days, and planting the atractylides macrocephala koidz and applying 0.5 to 3.5kg of biological organic fertilizer per 5 square meters; and irrigating the root of the atractylides macrocephala koidz once by using 500 to 1,000 times of the prochloraz or the fluazinam or hymexazol before disease outbreak in June and July; or the planting method comprises the following steps of: ploughing and covering the thin film after the crop rotation of spring corn, irrigating until water is above the soil surface, keeping hermetic for 20 to 90 days, and uncovering the thin film to plant the atractylides macrocephala koidz. By the planting method, the incidence rate of diseases such as root rot, southern blight and the like of the atractylides macrocephala koidz is reduced greatly, the seedling survival rate of the atractylides macrocephala koidz is improved, and the yield of atractylides macrocephala koidz rhizomes is improved. Simultaneously, the utilization rate of land can be improved to improve economic benefits.

The invention relates to a pesticide, in particular to a complex bactericide for mainly treating grey mould disease, early blight and powdery mildew, which is used for preventing and controlling the grey mould disease, early blight, powdery mildew, downy mildew and late blight of vegetable and fruit crops. The complex bactericide consists of active ingredients, a filler and/or an auxiliary agent, wherein the active ingredients consist of boscalid and methoxyl acrylate bactericide in the weight ratio of 1-100:1-100. The complex bactericide can also be prepared into wettable powder or water dispersible granules for use. The complex bactericide has the advantages of high quick acting performance, novel action mechanism and capability of effectively suppressing and delaying the occurrence and development of germ resistance to the medicament; and a preparation process has the advantages of simplicity, low cost, wide disease prevention spectrum and high biological safety.

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.

The present invention is method of controlling bakanae disease of wheat, barley, etc. with resistance to benzimidazole and similar germicides. Tebuconazole, nuarimol, imazalil, etc. are used alone or compounded, or the preparation with tebuconazole, nuarimol, imazalil, etc. as effective components is re-compounded with other systematic one protective germicide to form binary or polynary preparation for treating seed or field spraying. The present invention has obvious synergistic effect in preventing and treating bakanae disease of wheat, barley, etc. and can delay the generation of chemical resistance.

A novel strain of Bacillus subtilis EB120 shows high antagonistic activity for controlling various plant diseases including barley powdery mildew, cucumber powdery mildew, red pepper anthracnose, rice blast, tomato gray mold, tomato late blight and wheat leaf rust, it can be effectively used as a microbioside for biologically controlling the plant diseases.

The invention relates to a biological control agent, a bioorganic fertilizer, and applications thereof. The biological control agent comprises Bacillus velezensis, Bacillus subtilis, Paenibacillus polymyxa, and Bacillus amyloliquefaciens. The provided biological control agent and bioorganic fertilizer can continuously inhibit fusarium oxysporum for a long term, and has a long-lasting control effect on plant blight, in particular, banana blight.

The invention discloses identification and application of a biocontrol bacillus strain, and belongs to the technical field of agricultural microorganisms. Biocontrol bacillus is bacillus velezensis YJC-9, the strain is already reserved in The China Typical Culture Collection Center of Wuhan university at 11thApril in 2018, and the preservation serial number of the strain is CCTCC NO.M 2018196. Thebiocontrol bacillus strain can prevent and control wheat sharp eyespot, wheat scab, sclerotinia rot of colza, gray mold of strawberries, pepper root rot, kidney bean blight and tobacco alternariaalternata, is a biocontrol bacterium having broad-spectrum resistance, and has good application prospects in agricultural disease prevention and control.

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

The invention relates to the technical field of traditional Chinese herbal medicine cultivation, in particular to a herbahouttuyniae cultivation method, which comprises the following steps of: 1) determining a planting time: planting seedlings at a period from the middle ten days and last ten days of September to the first ten days of November every year; 2) determining a planting mode: selecting high-stalk crops for interplanting; 3) preparing the seedlings: conducting asexual reproduction by using subterraneous stems of herbahouttuyniae as reproduction materials; 4) planting the seedlings: directly placing the seedlings on compartment surfaces and covering the compartment surfaces with rice straw or maize stalks after the seedlings are planted; 5) conducting field management: removing weed at the growth period of the herbahouttuyniae, removing ill herbahouttuyniae plants, weak herbahouttuyniae plants and non-herbahouttuyniae crops during weeding, and making sure that the herbahouttuyniae is not damaged; and during top dressing, using farm manure as main fertilizers and chemical fertilizers as auxiliary fertilizers, paying attention to reasonable drainage and irrigation and keeping soil wet at the entire growth period of the herbahouttuyniae; and 6) harvesting at a proper time and the like. By adopting the herbahouttuyniae cultivation method, the yield of the herbahouttuyniae is high, the damage by diseases and insects is reduced, the herbahouttuyniae diseases such as southern blight and zonate spot can be effectively prevented and controlled, and the yield of the herbahouttuyniae and the mass content of medicinal components in the herbahouttuyniae are improved to the utmost extent.

The present invention relates to plant disease prevention and control with plant extract, and is especially purplestem eupatorium extract for plant disease prevention and control. The n-hexane and petroleum ether extract of purplestem eupatorium has high inhibiting activity on the pathogenic bacteria causing foot rot of broad bean, early blight of tomato, rice blast and pear scab. The purplestem eupatorium extract may be used to prepare germicide with other natural plant extracts, and the germicide has excellent prevention and control effect on plant diseases. The present invention utilizes purplestem eupatorium extract in preventing and controlling plant diseases for the first time.

The invention provides a machine vision technology based pre-warning system and method for pest and disease damage of crops in greenhouses. According to the system, image monitoring data and environment parameters under the natural background are acquired by an in-situ Canon EOS7D camera image sensor and multiple environmental sensors, a dual pest and disease damage pre-warning system realizing warning source pre-warning and warning sign pre-warning is constructed, and meanwhile, an information processing function that a user can inquire pre-warning records at any time is realized. Real-time environmental parameters and environmental parameters suitable for crop growth are matched firstly to provide preliminary pre-warning, real-time image data are processed through filtering, segmentation, feature extraction and identification and classification with an image processing technology in machine vision, so that tomato early blight and leaf mold are automatically identified and classified, the pest and disease damage morbidity situation of crops at present is provided, and decision support and historical information record inquiry functions are supplied to the user. Manpower and material resources are greatly reduced, and meanwhile, the pest and disease damage management efficiency of the crops in the greenhouse is further improved.

The invention discloses an application of tea saponin and mixture thereof in antisepsis and preservation of fruit, overcomes the shortages of the existing tea saponin in the antispesis and preservation of the fruit, provides the wide application of tea saponin in the antisepsis and preservation technical field of fruit and relates to the antisepsis and preservation application of various fruits and the control effects on post-harvest diseases of fruits such as penicillium italicum of citrus, green mold of citrus, Alternaria citri, banana crown rot, banana anthracnose, litchi blossom blight, lichi anthracnose, loquat anthracnose, loquat black spot or loquat gray spot, and the like. The tea saponin and the mixture thereof have safe use, meet the requirement of agricultural sustainable development, effectively reduce the harm of chemical agents on human being, and provide technical supports for good prospect of the application of the tea saponin and the mixture thereof in the field of antisepsis and preservation of the fruit.