208 results about "Blight" patented technology

The inventive technique process of grainy calcium material for preventing and treating peanut blight primarily includes: adopting the shell powder and weathered coal as raw materials, machining into peanut blight prevention material through agglomeration granulation. The invention has innovative and reasonable design, which not only has simple process, easy implementing, but also has low cost of raw materials, product application convenience, remarkable peanut blight prevention effect, and capability of promoting high-efficient production pf peanut. In particular, the invention applies coastal oyster shell wastes for machining calcium materials for preventing and treating peanut blight, which not only reduces environmental pollution, but also promotes recycling of resources, has very significant economic, social and ecological benefits and greater promotional value.

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention discloses pseudomonas chlororaphis S7-24 for antagonizing xanthomonas oryzae and application of the pseudomonas chlororaphis S7-24, and belongs to the technical field of microorganisms. The pseudomonas chlororaphis S7-24 is preserved in the China Center for Type Culture Collection on November 12, 2025, and the preservation number of the pseudomonas chlororaphis S7-24 is CCTCC NO: M 20252533. The pseudomonas chlororaphis S7-24 provided by the invention is a newly found pseudomonas biocontrol resource, and verification shows that the strain S7-24 has relatively high antagonistic activity on rice bacterial blight and rice bacterial streak, and can be used for effectively preventing and treating rice bacterial blight and rice bacterial streak. The pseudomonas chlororaphis S7-24 provided by the invention is used for preventing and treating rice bacterial leaf blight and rice bacterial leaf streak, can reduce the use amount of chemical pesticides, ensures grain safety, and has better economic and ecological benefits.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a biological product for overexpressing an OsARF7 gene to improvement of rice disease resistance, the nucleotide sequence of the OsARF7 gene is shown as SEQ ID NO.1, and the amino acid sequence of the OsARF7 gene is shown as SEQ ID NO.2. According to the invention, an OsARF7 overexpression rice strain is constructed by means of transgenosis. Tests show that the overexpression of the OsARF7 can significantly enhance the immunocompetence of the rice, for example, the expression level of a disease-resistant related gene is improved, active oxygen outbreak is significantly induced, and the resistance of the rice to false smut and bacterial leaf blight is significantly improved. The invention proves that the OsARF7 has a positive regulation effect on rice immunity, can be used for improving the disease resistance of rice, and has important significance for creating disease-resistant rice germplasm resources.

The invention discloses bacillus tequilensis 83IBC04-2 and application thereof, the bacillus tequilensis 83IBC04-2 comes from a deep sea hydrothermal zone and is preserved in China Center for Type Culture Collection on October 15, 2025, and the preservation number is CCTCC NO: M20252212. The compound has a remarkable antagonistic effect on a plurality of important pathogenic fungi such as tomato gray mold, tomato early blight, rice blast and wheat scab, can be used for preparing a broad-spectrum and high-efficiency biological bactericide, effectively reduces the use amount of chemical pesticides, and reduces pesticide residues and environmental pollution.

The invention discloses an application of a rice bHLH transcription factor OsbHLH024 and a coding gene thereof in disease-resistant breeding, and belongs to the technical field of gene engineering. The amino acid sequence of the rice bHLH transcription factor OsbHLH024 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the gene OsbHLH024 is as shown in SEQ ID NO. 1. Experiments prove that through overexpression of the OsbHLH024 gene in the Zhonghua 11, the resistance of rice to rice blast, banded sclerotial blight and leaf blight can be remarkably improved; the OsbHLH024 gene is knocked out, and the resistance of rice to rice blast, banded sclerotial blight and leaf blight can be remarkably reduced. Therefore, the OsbHLH024 gene can positively regulate the disease resistance of the plant, which is of great significance to the cultivation of disease-resistant rice varieties.

The invention belongs to the technical field of gene engineering, and discloses application of an F-box protein gene OsFBX235 in improvement of bacterial blight resistance. The invention specifically discloses an application of knocking out OsFBX235 gene in improving the bacterial blight resistance of rice or cultivating a bacterial blight resistant rice variety. The F-box protein coding gene OsFBX235 is separated and cloned from rice, and the gene is proved to participate in the defensive reaction of the rice to the bacterial blight for the first time through functional analysis, and is an important negative regulation factor for regulating the resistance of the rice bacterial blight. The gene OsFBX235 is knocked out through target gene screening in combination with a CRISPR / Cas9 technology, and the importance of the gene OsFBX235 on resistance regulation and control of the rice bacterial blight is proved. Meanwhile, knockout of the gene can be used for creating rice germplasm resources with high bacterial leaf blight resistance and can be applied to breeding of new varieties with the bacterial leaf blight resistance of the rice.

The application discloses a marine source penicillium and a fermentation method and application thereof. The marine source penicillium is named Penicillium sp. DSF059, and the preservation number is CCTCC NO: M 20232310. Penicilliu The application optimizes the culture condition of the strain, selects a culture medium and culture condition with high yield of antibacterial active compounds to carry out fermentation, so that more target products can be obtained, the operation is simple, the cost is low, and meanwhile, the marine source penicillium Penicilliu Penicillium sp. DSF059 can provide an excellent strain for new drug development of resisting plant pathogenic fungi such as coffee leaf blight, coffee brown spot disease, grape gray mold disease, Chinese cinnamon brown spot disease, citrus black rot disease, pepper late blight, hickory leaf blight, coffee black spot disease and four pathogenic fungi separated from coffee leaf diseases and Staphylococcus aureus, and has a good application prospect for preventing and treating the plant pathogenic fungi and Staphylococcus aureus.

The invention discloses a method for cultivating a heat-resistant broad-spectrum durable rice blast-resistant rice breeding material based on polygene polymerization. According to the method, a high-heat-resistance and high-dominance indica-japonica hybrid indica rice restorer line is selected as a recurrent parent, a breeding intermediate material for polymerizing complementary broad-spectrum rice blast resistance genes Pigm and Pi-jx is used as a donor parent, and polymerization of the heat-resistance and broad-spectrum lasting rice blast resistance genes Pigm and Pi-jx is realized through a method of combining molecular breeding with conventional breeding. Meanwhile, by selecting representative strains to carry out artificial inoculation identification of seedling blast and panicle blast, natural induction identification of a rice blast retransmission area and heat resistance identification, and by combining main agronomic trait evaluation in the whole growth period, a heat-resistant broad-spectrum durable rice blast-resistant breeding material is obtained. The breeding material bred by using the method disclosed by the invention has relatively strong heat resistance and broad-spectrum and lasting rice blast resistance, and the breeding process of heat-resistant and rice blast-resistant rice varieties is accelerated.

The invention belongs to the field of molecular biology, and particularly relates to application of an OsNiR1 gene to improvement of rice broad-spectrum disease resistance and improvement of rice agronomic traits. The invention provides an application of an OsNiR1 gene, and the application comprises an application in preventing and treating plant diseases and / or enhancing the resistance of plants to pathogenic bacteria, and / or an application in improving agronomic traits of the plants. The amino acid sequence coded by the OsNiR1 gene is as shown in SEQ ID NO. 1, and the CDS sequence of the OsNiR1 gene is as shown in SEQ ID NO. 2. The application proves that OsNiR1 participates in regulating and controlling the immune response pathway of rice to fungal disease rice blast, fungal disease banded sclerotial blight and bacterial disease bacterial leaf blight. Meanwhile, after the strong promoter ubi is adopted for driving, an overexpression material of the OsNiR1 is higher and stronger than a wild plant type, and the tiller number is increased. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The invention discloses a potato late blight antagonistic bacterium and application thereof, belongs to biological agents, and provides more strain resources for prevention and treatment of potato late blight. The potato late blight antagonistic bacterium is Pseudomonas chlororaphis blT-49 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 27, 2021, and the preservation number is CGMCC No.24203. The invention further discloses a preparation method of the potato late blight antagonistic bacterium. The strain disclosed by the invention not only can inhibit the occurrence and growth of potato late blight, but also can regulate and control the ecological system of the micro-ecology of potatoes, reduce the infection ability of potato late blight and improve the disease resistance of potatoes. In the growth and development of the potatoes, the growth and development of the potatoes can be promoted, and the single-plant yield is improved.

The application discloses application of BoDMR1 and BoDMR6 genes in cultivating disease-resistant broccoli varieties, an expression vector and a cultivating method, and relates to the technical field of plant molecular biology. A nucleotide sequence of a broccoli BoDMR1 gene is shown in SEQ ID No: 7, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 7; and a nucleotide sequence of a broccoli BoDMR6 gene is shown in SEQ ID No: 8, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 8. Through gene editing on endogenous BoDMR1 and / or BoDMR6 genes of plants, mutant plants showing resistance to diseases such as sclerotinia, black rot and alternaria blight can be obtained. Therefore, the application is beneficial to providing good resources for cultivating disease-resistant broccoli varieties.

The invention discloses application of m-cresol to prevention and treatment of pepper southern blight, and belongs to the field of plant disease prevention and treatment. According to the invention, it is found for the first time that m-cresol has an inhibition effect on sclerotium rolfsii, wherein EC50 of m-cresol is 0.1560 [mu] L / mL; the m-cresol with the concentration of 2 * EC50 can completely inhibit sclerotium germination, sclerotium generation and hypha growth of the sclerotium rolfsii. Furthermore, m-cresol can be used for preventing and treating pepper southern blight caused by sclerotium rolfsii, the morbidity and disease index of pepper southern blight can be effectively reduced by using a m-cresol solution with the concentration of 0.125% to perform root irrigation treatment on pepper planted in pathogenic bacteria soil, the relative prevention and treatment effect reaches 100%, and adverse reaction of pepper plants cannot be caused. The invention provides a new method for controlling the pepper southern blight by using a medicament, and the method has the advantages of high efficiency, low cost, low environmental harm and the like.

The invention relates to a dispersible oil suspending agent for preventing and treating potato late blight and a preparation method and application thereof, and relates to the field of pesticides, the dispersible oil suspending agent comprises the following raw materials by weight: 5-25% of mandipropamid, 5-25% of cymoxanil, 5-20% of a dispersant, 5-20% of an emulsifier, 2-5% of sodium carboxymethyl cellulose, and 27-65% of solvent oil 200 #. Field control experiments show that the dispersible oil suspending agent for preventing and treating potato late blight can effectively prevent and treat potato late blight, is safe and remarkable in prevention and treatment effect, and has no adverse effect on other diseases and insect pests and no adverse effect on non-target organisms.

This invention discloses an oxyindene compound containing substituted pyridine and its application as a fungicide. The oxyindene compound containing substituted pyridine has broad-spectrum fungicidal activity, especially effective in controlling rice sheath blight, apple tree rot, rapeseed sclerotinia stem rot, wheat scab, pepper blight, cucumber anthracnose, tomato gray mold, corn curvature blight, rice blast, tomato early blight, wheat take-all, and wheat stem rot. It can be used as a fungicide for the prevention and control of the above-mentioned plant diseases.

The invention belongs to the technical field of phytopathogen detection, and particularly relates to a specific detection primer and a specific detection kit for Cercospora cf.flagellaris, and an application of the specific detection primer and the specific detection kit for Cercospora cf.flagellaris. By adopting the detection primer or the kit disclosed by the invention, the specific detection of the phytolacca acinosa can be realized, and the detection primer or the kit has good intraspecific strain universality. Meanwhile, through a sensitivity experiment, the detection system and the detection method established by the invention have the lowest detection concentration of 0.012 pg.L <-1 > aiming at the phytolacca acinosa. The detection system is used for detecting soybean grains with purple spot symptoms in the field, and can be used for quickly and accurately identifying whether soybean plants carry pathogenic bacteria of sporospora phytolacca or not. Therefore, the detection primer and the kit provided by the invention have good application prospects.

The invention discloses a transcription factor AcWRKY84 gene for regulating and controlling gray mold resistance of kiwi fruits. After the expression of the gene is silenced, the gray mold resistance of kiwi fruits can be remarkably reduced. The AcWRKY84 gene is cloned, and the anti-gray mold effect of the AcWRKY84 gene in kiwi fruits is proved, specifically, after an experimental group is inoculated with viruses, compared with a control group, the disease symptoms of the fruits of the experimental group are aggravated, the infected area is obviously enlarged, and the enzyme activities of SOD, POD and CAT in the fruits are obviously reduced compared with the control group; sufficient gene resources can be provided for a kiwi fruit gray mold resistance gene pool, so that the possibility of successfully breeding high-gray mold-resistance varieties subsequently is greatly increased.

The invention discloses a method and a system for detecting rice bacterial leaf blight based on unmanned aerial vehicle spatial spectrum fusion. The method comprises the following steps: firstly, constructing a rice bacterial leaf blight detection data set; a rice bacterial leaf blight severity grading detection double-branch model is constructed, and the model adopts a spectrum-space double-branch parallel coding and fusion framework and comprises a spectrum branch module, a space branch module and a cross attention fusion mechanism module. And inputting the complete image of the rice field into the trained rice bacterial leaf blight severity grading detection double-branch model, and obtaining severity grading detection results of the bacterial leaf blight in different rice growth periods of the target field. According to the invention, deep complementation and dynamic information balance of spectrum and spatial features of the multispectral unmanned aerial vehicle are realized, so that the accuracy of disease detection and the generalization ability of the model are effectively improved.

The application discloses a SNP molecular marker for identifying or assisting in identifying rice bacterial leaf blight resistance and a primer composition thereof. The method comprises detecting polymorphism or genotype (allele) of an SNP site in a genome of a rice to be detected, and identifying or assisting in identifying rice bacterial leaf blight resistance according to the genotype, wherein the SNP site is a site on a chromosome 5 of the rice, and the nucleotide type is A or G, which is the 30th nucleotide in sequence 4 in a sequence table. The method can be used for predicting rice bacterial leaf blight resistance and rice breeding. The substance for detecting the polymorphism and genotype of the SNP site can be combined with other substances (such as a substance for detecting single nucleotide polymorphism or genotype of other molecular markers related to rice bacterial leaf blight resistance) to prepare a product for identifying rice bacterial leaf blight resistant varieties.

The application relates to the technical field of intelligent agriculture and plant protection, and particularly discloses a broccoli black spot early diagnosis system and method based on multi-modal machine learning fusion, which comprises a portable collection platform, a mobile experiment terminal and a server. The portable collection platform is integrated with a hyperspectral imaging camera, a chlorophyll fluorescence imager, a dark-adapted leaf clamp and a camera for sample barcode recognition, and is provided with an adjustable height support and a controllable light source to ensure the consistency of data collection conditions. The mobile experiment terminal is internally provided with a physicochemical index rapid detection module, including a portable PCR instrument for on-site nucleic acid rapid extraction and amplification and a miniature ultraviolet spectrophotometer for rapid determination of pigment content. The broccoli black spot early diagnosis system and method based on multi-modal machine learning fusion are highly integrated, portable and automated, are suitable for a field environment and provide an efficient disease diagnosis tool for non-expert users.

The application belongs to the technical field of apple molecular breeding, and particularly relates to application of a detection reagent of a molecular marker related to apple anthracnose leaf blight resistance, the molecular marker being Del772, Del231 and SNP947, the nucleotide sequence of Del772 being shown as SEQ ID NO. 1, the nucleotide sequence of Del231 being shown as SEQ ID NO. 4, and the nucleotide sequence of SNP947 being shown as SEQ ID NO. 5, the application further develops the detection reagent of the molecular markers Del772, Del231 and SNP947, the detection reagent can detect or identify the resistance of apples to anthracnose leaf blight, and a method for identifying the resistance of apples to anthracnose leaf blight is developed, thereby providing a new way for breeding disease-resistant apples.

The invention provides a penicillium oxalicum strain K3R2 and a preparation method and application of a biological agent of the penicillium oxalicum strain K3R2. The biocontrol bacterium Penicillium oxalicum strain K3R2 screened by the invention is preserved in the China General Microbiological Culture Collection Center on September 25, 2025, the preservation number is CGMCC NO.42219, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing; the ITS sequence of the Penicillium oxalicum strain K3R2 is as shown in SEQ ID NO: 1, and the CaM sequence is as shown in SEQ ID NO: 2. The penicillium oxalicum strain K3R2 provided by the invention can inhibit hypha growth and spore germination of beet neocam spores, the hypha inhibition rate is 83.60%, the spore inhibition rate is 65.31%, and the penicillium oxalicum strain K3R2 has the effect of preventing and treating quinoa stem blight. Meanwhile, the penicillium oxalicum strain K3R2 has the capabilities of salt resistance, alkali resistance, phosphate solubilization, potassium solubilization, cellulose solubilization and nitrogen fixation; the strain K3R2 remarkably promotes the growth of quinoa plants, so that the chlorophyll content is increased by 20.22%, and the accumulation of nutrient substances is accelerated; the fresh weight, the dry weight and the stem diameter of quinoa plants are increased, and the lodging resistance is enhanced.

The invention provides a spathiphyllum conifer strain R3, a complex microbial inoculant thereof and application of the spathiphyllum conifer strain R3 in prevention and control of late blight, and belongs to the technical field of biocontrol bacteria. The invention relates to a spathiphylli strain R3, and the preservation number of the spathiphylli strain R3 is CGMCC (China General Microbiological Culture Collection Center) No.36025. The spathiphylli strain R3 has the advantages that the preservation number is CGMCC No.36025; the bacterial strain R3 has good bacteriostatic activity on phytophthora infestans, and meanwhile, the biocontrol agent formed by compounding the bacterial strain R3 with at least one of the bacillus safensis bacterial strain B1 and the leisei bacterial strains L1 and L3 or the combination of the bacterial strain R3 and the L6 has good prevention and control effects in pot experiments and field experiments.

The application discloses an endophytic fungus LH001 and application thereof, and belongs to the technical field of plant disease control. The endophytic fungus LH001 is separated from the root system of wild rice, is classified and named as Pseudophialophora sp. LH001, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M 20241751. The endophytic fungus LH001 strain is co-cultured with rice, is allowed to colonize the root of rice seedlings, can reduce the leaf damage caused by Magnaporthe oryzae, enhances the disease resistance of rice seedlings to leaf blight, and the control effect reaches 94.05%. Therefore, the biological control effect of the endophytic fungus LH001 on rice blast has great value in the popularization and application in the agricultural field.

The invention discloses application of pseudopenicillin variotii in preparation of a resistance inducer for preventing and treating plant diseases, and belongs to the technical field of plant protection. The invention provides an application of Panax variotii in prevention and treatment of plant diseases, the application concentration of the Panax variotii is 0.1 microgram / mL-1 microgram / mL, a plant resistance inducer containing the Panax variotii can be sprayed on plant leaves or fruit surfaces and has a remarkable prevention effect on anthrax leaf blight, and the higher the concentration is, the better the prevention effect is; the pseudopenicillin variotii can promote synthesis or activity improvement of catalase, peroxidase, polyphenol oxidase, glutathione S-transferase and superoxide dismutase in apple bodies, and also can remarkably induce up-regulation expression of disease-resistant related genes such as MdPR2 and MdPR5, so that the disease resistance, the antioxidant level and the plant immunity of apples are enhanced; a novel and efficient plant resistance inducer application scheme is provided for apple disease control and physiological regulation.

The application discloses a bactericide and application thereof in prevention and treatment of stem blight of asparagus, and belongs to the technical field of plant disease prevention and treatment. The bactericide for preventing and treating stem blight of asparagus is prepared from the following raw materials in parts by weight: psyllium extract 20-30 parts, asarum extract 8-15 parts, poria cocos extract 8-15 parts and gallnut extract 40-60 parts. The bactericide prepared from Chinese herbal medicine raw materials effectively inhibits the occurrence and development of stem blight of asparagus, reduces environmental pollution and food safety problems, and has important significance for improving the yield of asparagus and economic benefits of asparagus planting.

This invention discloses the application of the OsCAD3 protein and its encoding gene in regulating resistance to bacterial blight in rice, relating to the field of plant genetic engineering. The amino acid sequence of this gene protein is shown in SEQ ID NO.3. Cloning the gene editing site sequence, constructing a gene editing recombinant vector, and transforming it into rice can improve rice resistance to bacterial blight and ensure stable rice yield. The OsCAD3 gene editing vector constructed in this invention and the new OsCAD3 allele formed through genome editing can be applied to rice breeding for resistance to bacterial blight. Constructing an overexpression recombinant vector of the above gene coding region sequence and transforming it into rice can reduce rice resistance to bacterial blight and ensure stable rice yield. The above gene overexpression vector can be used in rice breeding to regulate bacterial blight. This invention can be used in the fields of rice germplasm resource improvement and genetic breeding, and is of great significance for breeding new rice varieties resistant to bacterial blight.

The application of an endogenous yeast and its application in resisting apple botrytis blight belongs to the field of microorganism technology. In order to solve the technical problems that apple is easily infected by botrytis cinerea to cause botrytis blight during transportation and storage, and the adverse effects of chemical fungicides on environment and human health, the application screens an endogenous yeast from apple, which is identified as Bullera alba through morphological and molecular biological analysis. The experiment proves that the cell suspension of the strain PGY-2 has a control effect on postharvest botrytis blight of apple, and can well colonize at the apple wound, can antagonize the botrytis blight pathogen by forming a biofilm and inducing the activity of various defense enzymes, and the strain PGY-2 can be used for preparing a fungicide for preventing and treating postharvest botrytis blight of apple.

The application provides a group of genes for regulating resistance of tomatoes to botrytis blight, and the genes are tomato SlBIUPa gene and tomato SlBIUPb gene; wherein the nucleotide sequence of the tomato SlBIUPa gene is shown in SEQ ID NO. 1, or has more than 90% homology with the nucleotide sequence shown in SEQ ID NO. 1, and the nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO. 2; the nucleotide sequence of the tomato SlBIUPb gene is shown in SEQ ID NO. 3, or has more than 90% homology with the nucleotide sequence shown in SEQ ID NO. 3, and the nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO. 4. SlBIUPa and SlBIUPb can negatively regulate resistance of tomatoes to botrytis blight, the functions of the two genes in regulating resistance of tomatoes to botrytis blight have a dose effect, and knocking out SlBIUPa or SlBIUPb alone can reduce the lesion area, and knocking out SlBIUPa and SlBIUPb simultaneously can more obviously enhance the resistance to botrytis blight.