575 results about "Blight" patented technology

The present invention relates to the technical field of pesticides, and discloses a biological agent-based rice sheath blight disease efficient control agent, which comprises an active flora, a carrier, an auxiliary agent and a disease resistance enhancing factor, the active flora is used for combining humidity, pH and temperature response type materials so as to make the bacterial agent dynamically released, and the disease resistance enhancing factor is used for controlling the disease resistance of the rice sheath blight disease. The carrier is used for enabling the active ingredients to be slowly released in a field environment and keep stability, the auxiliary additive is used for improving the activity, adhesiveness and soil adaptability of the fungicide, and the disease-resistant enhancing factor is used for expanding and improving the adaptability of the control agent. The ratio of the active flora to the carrier to the auxiliary additive to the disease-resistant enhancing factor is 10: 50: 13: 27. Through the synergistic effect of various functional strains and the combination of the dynamic release carrier, the occurrence and propagation of the rice sheath blight disease are effectively inhibited, the prevention and treatment rate can reach 75-85%, and the prevention and treatment effect is obviously higher than that of a traditional chemical pesticide or a single fungicide.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention discloses a microbial agent for preventing and treating soil-borne diseases of food crops and a preparation method of the microbial agent, and belongs to the technical field of microorganisms. The microbial inoculum comprises three functional strains, namely pseudomonas guayensis, paenibacillus polymyxa and trichoderma koningiopsis, the three strains are mixed in equal proportion and cooperate with one another, so that the microbial inoculum has a remarkable disease-resistant effect, has an antagonistic effect on various diseases of wheat and corn, can be used for preventing and treating wheat root rot, take-all and banded sclerotial blight as well as corn stem rot and banded sclerotial blight, and has a wide application prospect. Particularly, the inhibition effect on the take-all disease of wheat is optimal. Meanwhile, the coating agent and the auxiliaries are used, so that the acting durability and stability of the microbial inoculum are improved. The microbial agent is matched with a conventional base fertilizer for use, use of chemical bactericides is reduced, growth of common pathogenic bacteria of food crops is effectively inhibited, and the yield of the food crops is increased.

The invention belongs to the field of biological prevention and control of plant diseases, and particularly discloses a biocontrol bacterium bacillus velezensis and application thereof in preparation of a biocontrol inoculant for preventing and controlling pepper phytophthora blight, the strain is bacillus velezensis Y426-7, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 23, 2024, and has the preservation number of CGMCC NO.32041, and the preservation number is CGMCC NO.32041. The biocontrol bacterium is bacillus velezensis Y426-7. The bacillus velezensis is preserved in No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and can be used for preparing a biocontrol inoculant for preventing and treating pepper phytophthora blight. Compared with the prior art, the bacillus velezensis Y426-7 obtained by the invention has an obvious antibacterial effect on phytophthora capsici and various crop phytophthora blight bacteria under an indoor in-vitro condition; furthermore, the 2.5 * 10 < 9 > CFU / g wettable powder prepared from the bacillus velezensis Y426-7 has a relatively good prevention and treatment effect and a certain yield increasing effect on the pepper phytophthora blight in the field.

The invention discloses a corn NAC transcription factor and application thereof in enhancing disease resistance. The corn NAC transcription factor can endow transgenic rice with wide resistance and does not affect the rice yield, and the amino acid sequence of the corn NAC transcription factor is SEQ ID No.2. The sequence of the coding gene of the gene is SEQ ID No.1. Research shows that the corn NAC transcription factor is located in a cell nucleus, the resistance of rice to rice blast caused by magnaporthe oryzae and rice bacterial leaf blight caused by Xanthomonas oryzae can be improved by overexpression of the corn NAC transcription factor in rice, meanwhile, the agronomic traits of the rice are evaluated, it is found that the gene does not affect the ear length and the grain length of the rice, and the corn NAC transcription factor can be used for improving the rice resistance to rice blast caused by magnaporthe oryzae and the rice bacterial leaf blight caused by Xanthomonas oryzae. Thousand seed weight and single plant yield.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention discloses an apple anthracnose leaf blight recognition five-dimensional data fusion method based on a patrol robot, relates to the technical field of anthracnose leaf blight recognition, and solves the problems of limited sensing dimension, large illumination interference and inaccurate spatial positioning in the prior art. Comprising the following steps: S1, acquiring surface curvature characteristics of apple leaves, collecting RGB-D images of the leaves, constructing a three-dimensional topological structure of the leaves, and positioning scab space distribution; s2, acquiring spectral reflection information of apple leaves, quantifying biochemical components of chlorophyll a and carotenoid, and constructing a vegetation index feature set sensitive to diseases; s3, apple tree canopy temperature information is collected, a transpiration anomaly detection model is constructed, and multi-modal fusion of thermal infrared and point cloud data is realized; and S4, integrating spatial distribution, spectral reflection and thermal radiation information, constructing a five-dimensional phenotypic characteristic matrix model, and comprehensively analyzing apple phenotypic characteristics. According to the method, the recognition accuracy under the conditions of blade shielding, uneven illumination, blade posture change and environment interference can be remarkably improved.

The inventive technique process of grainy calcium material for preventing and treating peanut blight primarily includes: adopting the shell powder and weathered coal as raw materials, machining into peanut blight prevention material through agglomeration granulation. The invention has innovative and reasonable design, which not only has simple process, easy implementing, but also has low cost of raw materials, product application convenience, remarkable peanut blight prevention effect, and capability of promoting high-efficient production pf peanut. In particular, the invention applies coastal oyster shell wastes for machining calcium materials for preventing and treating peanut blight, which not only reduces environmental pollution, but also promotes recycling of resources, has very significant economic, social and ecological benefits and greater promotional value.

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention belongs to the technical field of biological control, and particularly relates to a pseudomonas flax DY-31 strain, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the pseudomonas lini (Pseudomonas lini) DY-31 is CGMCC (China General Microbiological Culture Collection Center) No.34088. The pseudomonas lini (Pseudomonas lini) DY-31 is The pseudomonas flax DY-31 or the biocontrol inoculant thereof is applied to prevention and treatment of plant soil-borne diseases, specifically including peanut black rot, peanut southern blight, peanut sclerotinia rot, peanut leaf rot sclerotinia rot, peanut basal rot and wheat stem basal rot. The pseudomonas flax DY-31 provided by the invention is an important strain resource, can be used for creating a biopesticide preparation, and is used for green prevention and treatment of soil-borne diseases of peanuts and wheat. In addition, the DY-31 strain also has various growth promoting functions, and has important development and application values in the aspects of agricultural biological control and plant growth promoting.

The invention discloses burkholderia gladioli and an application of the burkholderia gladioli. The invention provides a Burkholderia gladioli HZX-8 strain, and the preservation number of the Burkholderia gladioli HZX-8 strain is CGMCC (China General Microbiological Culture Collection Center) No.33009. The Burkholderia gladioli HZX-8 strain has the advantages that the The invention also provides an application of the strain or a culture solution or a bacterial solution or a fermentation culture solution of the strain in preventing or treating plant walnut anthracnose and / or walnut branch blight. The Burkholderia gladioli HZX-8 strain disclosed by the invention has the advantages that the Burkholderia gladioli HZX-8 strain obtained by the invention shows a remarkable biological control potential in the aspects of preventing and treating the walnut anthracnose caused by the Colletotrichum guangyuanense and the walnut branch blight caused by the Diapotheca chaotianensis, and the Burkholderia gladioli HZX-8 strain can be used for preventing and treating the walnut branch blight caused by the Colletotrichum guangyuanense and the Burkholderia gladioli HZX-8 strain can be used for preventing and treating the walnut branch blight caused by the Diapotheca chaotianense.

The invention relates to the technical field of gene isolation and plant improvement, in particular to a late blight resistance gene, a biological material and application. The Rpi-card1 nucleic acid molecule provided by the invention comprises a nucleotide sequence as shown in any one of SEQ ID NO: 1-4; a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 5 or 6; the nucleotide sequence has at least 75% sequence identity with the nucleotide sequence. The invention provides an important gene resource for potato late blight resistance breeding, overcomes the problem of disease resistance loss of potato varieties, and provides a new guarantee for lasting disease resistance of potatoes.

The invention provides a rice defense gene LOCOs02g41680 and application of the rice defense gene LOCOs02g41680 in bacterial leaf blight resistance, and relates to the technical field of modern biotechnology gene engineering. The rice defense gene LOCOs02g41680 disclosed by the invention has a nucleotide sequence as shown in SEQ ID NO.1, and consists of 2643 nucleotides; 2, the sequence length of the coding region sequence is 2142 nucleotides, and the coding region sequence is shown in SEQ ID NO. A protein with 713 amino acids is coded, and the amino acid sequence is shown in SEQ ID NO.3. The rice defense gene LOCOs02g41680 disclosed by the invention can be used for creating a rice disease-resistant material for resisting bacterial blight, the disease resistance of the rice can be improved when the rice defense gene LOCOs02g41680 is applied to the rice, and valuable gene resources can be provided for breeding new varieties of the rice for resisting the bacterial blight.

The invention relates to the technical field of biological control, and discloses bacillus siamensis and application thereof. The bacillus siamensis disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.32576. The bacillus siamensis has a good control effect on pepper phytophthora blight, the field control effect can reach 71.69 + / -8.00%, meanwhile, the bacillus siamensis can antagonize various plant pathogenic fungi in a broad spectrum, and the bacillus siamensis can be used for preventing and treating various plant diseases.

The invention belongs to the technical field of agricultural science, and particularly provides application of oligosaccharide in prevention and treatment of corn southern leaf blight. The oligosaccharide has an inhibiting effect on the bipolaris maydis and can be used as an inhibitor of the bipolaris maydis. Meanwhile, the oligosaccharide can be used as a plant immune resistance inducer for preventing and treating the corn southern leaf blight, and the occurrence and development of the corn southern leaf blight are prevented and controlled by inducing basic resistance and enhancing the plant immunity. The oligosaccharide application method is simple and convenient to operate, green and environment-friendly, green prevention and control are facilitated, and grain loss caused by diseases is reduced more economically and more environmentally.

The invention discloses Pseudomonas sp., a microbial agent prepared from the Pseudomonas sp. And application of the Pseudomonas sp. And the microbial agent prepared from the Pseudomonas sp. The pseudomonas strain N2-3-1-14 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC); the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation time is April 8, 2025; the preservation number is CGMCC 34128. The pseudomonas strain N2-3-1-14 and the microbial inoculum prepared from the pseudomonas strain N2-3-1-14 can effectively inhibit growth of pathogenic bacteria of pine blight and pine needle red spot, and the bacteriostasis rate is 65% or above. The strain can also significantly promote the growth of pinus sylvestris.

The invention discloses pseudomonas chlororaphis S7-24 for antagonizing xanthomonas oryzae and application of the pseudomonas chlororaphis S7-24, and belongs to the technical field of microorganisms. The pseudomonas chlororaphis S7-24 is preserved in the China Center for Type Culture Collection on November 12, 2025, and the preservation number of the pseudomonas chlororaphis S7-24 is CCTCC NO: M 20252533. The pseudomonas chlororaphis S7-24 provided by the invention is a newly found pseudomonas biocontrol resource, and verification shows that the strain S7-24 has relatively high antagonistic activity on rice bacterial blight and rice bacterial streak, and can be used for effectively preventing and treating rice bacterial blight and rice bacterial streak. The pseudomonas chlororaphis S7-24 provided by the invention is used for preventing and treating rice bacterial leaf blight and rice bacterial leaf streak, can reduce the use amount of chemical pesticides, ensures grain safety, and has better economic and ecological benefits.

The application discloses an NLR plug-in gene for improving plant disease resistance and application thereof, and belongs to the field of plant molecular biology and biotechnology.The NLR plug-in gene StEM1 has a whole gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO.3.The gene plays a key role in improving plant disease resistance, especially resistance to field late blight variant bacteria.In the NLR background, overexpression of the gene significantly promotes the disease resistance of tobacco and potato to pathogenic pythium, and the gene is a gene for enhancing plant disease resistance by improving ETI immunity.Overexpression of the gene in the NLR background is obtained by a genetic transformation method, and a strain line is obtained to resist field variant strains, so as to cope with field diseases of crops.The application provides an excellent gene resource for green prevention and control of late blight and disease-resistant genetic engineering breeding.

The invention belongs to the field of corn disease prevention, relates to a novel small peptide, provides a small peptide NCRP246 and application thereof in improvement of corn southern leaf blight resistance, and provides feasibility for plant disease resistance by using non-classical peptides. A corn southern leaf blight resistance identification experiment proves that NCRP246 can significantly improve the resistance of corn to southern leaf blight, and further researches that the NCRP246 reduces oxidative damage of pathogenic bacteria infection of the southern leaf blight to the corn by reducing H2O2 accumulation so as to mediate the resistance of the corn to the southern leaf blight; the analysis from the molecular perspective proves that the NCRP246 can induce the expression of defense related genes.

The invention relates to the technical field of microorganisms, in particular to a method for promoting corn growth to improve the resistance of plants to northern leaf blight of corn and application thereof.The method for promoting corn growth to improve the resistance of plants to northern leaf blight of corn comprises the following specific steps that S1, a test field is subjected to scribing treatment and divided into four test fields, each test field is divided into four ridges, the ridge width is 0.61 m, and the ridge width is 0.6 m; the ridge length is 15 m, the aisle between each cell is 2 m, and the front and rear protection rows of the test area are 10 m respectively; s2, a first area is a blank control area for normal planting of corn plants, a second area is a second area for drip irrigation of beauveria bassiana planting (Bb) for the corn plants, a third area is a third area for northern leaf blight inoculation planting (Et) for the corn plants, and a fourth area is a fourth area for drip irrigation of beauveria bassiana planting (Bb + Et) after northern leaf blight inoculation for the corn plants. Beauveria bassiana applied in a drip irrigation mode has good effects on corn growth and resistance.

The invention discloses application of a gene OsRGA2 to improvement of disease and insect pest resistance of rice, and the gene OsRGA2 can enhance the disease and insect pest resistance of rice to brown planthopper, bacterial leaf blight and the like, and is used for cultivating a new variety of disease and insect pest resistant rice.

The invention discloses an African trichoderma harzianum strain 6-5 and application thereof, the disclosed strain is named as Trichoderma harzianum, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 41930. The bacterial strain has obvious bacteriostatic activity on crop pathogenic bacteria such as colletotrichum capsici, apple cyst, botrytis cinerea, alternaria longibrachiata and phytophthora capsici, and fermentation liquor of the bacterial strain has good prevention and control effects on apple anthracnose leaf blight, apple bitter rot and apple tree canker.

The invention discloses a wheat leaf blight bacterium detection method based on an enzyme-mediated double-index amplification technology and a detection kit thereof. The invention belongs to the technical field of biology, and particularly relates to a wheat leaf blight bacterium detection method based on an enzyme-mediated double index amplification technology and a detection kit thereof. The composition for researching and detecting wheat leaf blight bacteria comprises a primer F5, a primer R3 and an RNA probe, the nucleotide sequence of the primer F5 is shown as SEQ ID No: 5, the nucleotide sequence of the primer R3 is shown as SEQ ID No: 9, and the nucleotide sequence of the RNA probe is shown as SEQ ID No: 16. According to the present invention, based on the EmDEA technology, the specific screening design is performed on the DNA primer and the RNA probe, such that the rapid detection method of the wheat leaf blight bacteria is established, and the method has good specificity, can detect the minimum DNA content of the wheat leaf blight bacteria of 100 fg, and has application prospects.

The invention discloses an application of Burkholderia gladioli XC-1 in prevention and treatment of plant diseases, the 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO: 3, and the plant diseases are litsea cubeba shoot blight, okra root rot, durian leaf spot disease, cubilose fruit canker, agilawood root rot or cassava leaf spot disease. The invention finds that the Burkholderia gladioli XC-1 has better development value and application potential in the prevention and treatment of various plant diseases, and a new microbial strain resource is provided for the prevention and treatment of various plant diseases.

The invention is suitable for the technical field of agricultural bactericides, and provides application of an echinocandin antifungal drug in prevention and treatment of agricultural diseases. According to the present invention, the two common echinocandin antifungal drugs such as the anidulafungin and the Rezafungin acetate, which are common in the field of medicine, are firstly applied to prevention and control of crop diseases; prevention and control tests are carried out on crop fungal diseases such as sclerotiniose, corn southern leaf blight, tomato gray mold, rice blast and crop anthracnose, and results show that the two drugs can significantly inhibit the growth of pathogenic fungal hyphae, and compared with an existing agricultural commercial bactericide carbendazim, the bactericidal composition shows higher inhibitory activity and has the characteristics of high efficiency and low toxicity. The invention lays a foundation for deep research and application of echinocandin drugs in the agricultural field, has a wide market prospect, effectively improves the crop disease prevention and control level, and promotes grain yield increase and quality safety.

The invention discloses a northern corn leaf blight early warning method and system based on data analysis, and relates to the technical field of agricultural disease early warning, and the main scheme is as follows: a data acquisition module acquires meteorological data, planting density data, pathogenic bacteria transmission data and physiological status data of corn growth in real time; the method comprises the following steps that: a data analysis module analyzes various data to obtain a meteorological influence coefficient # imgabs0 #, a planting density influence coefficient # imgabs1 #, a pathogenic bacterium propagation rate # imgabs2 # and a physiological state influence coefficient # imgabs3 #, an early warning issuing module calculates a northern leaf blight influence index # imgabs4 # according to the coefficients, whether risk early warning is triggered or not is judged and a protection instruction is generated, and a user interaction module receives early warning data and sends the early warning data to a control module. Early warning information and a data labeling graph are displayed, an early warning analysis report is generated, related personnel are notified through mails, short messages or systems, comprehensive, accurate and timely corn northern leaf blight early warning is achieved, and powerful guarantee is provided for agricultural production.

The present invention relates to the field of microbial technology, and in particular to a Velez bacillus HF01 and its application; Velez bacillus HF01 has been deposited as a patented strain in the General Microbiological Center of China Microbiological Culture Collection Administration Committee, with a deposit number of CGMCC NO: 33137. The strain has a significant effect in preventing and treating wax gourd damping-off disease caused by plant pathogens; it has a good inhibitory effect on wax gourd damping-off disease caused by Rhizoctonia solani, and can also promote the growth of wax gourd.

The invention belongs to the technical field of biological prevention and control of plant diseases, and provides bacillus subtilis and application thereof in prevention and control of corn diseases. The preservation number of the bacillus subtilis SEB-34 is CGMCC (China General Microbiological Culture Collection Center) No. 26419. The invention also provides a microbial agent produced by using the SEB-34 strain, and the microbial agent can be used for preventing and treating plant diseases caused by fusarium graminearum or pelamys maydis. The bacillus subtilis provided by the invention is applied through seed treatment before sowing or root drip irrigation after seedling emergence, has the effects of preventing diseases, promoting growth and increasing yield, and can effectively reduce the occurrence of corn southern leaf blight and basal stem rot. The microbial agent provided by the invention is in a semi-dormant state, has relatively strong stress resistance, is more stable to store, ensures the effect, and effectively reduces the production and transportation cost at the same time. The invention also provides beneficial microbial resources for green prevention and control of corn diseases, and has good application prospect and high application value.

The invention discloses a rice protein OsFBX388 related to plant disease resistance as well as a coding gene and application of the rice protein OsFBX388. The invention provides an application of OsFBX388 protein or a related biological material thereof in regulation and control of plant disease resistance, and also provides a substance for reducing the content or activity of the OsFBX388 protein, or a substance for reducing or inhibiting expression of a coding nucleic acid molecule of the OsFBX388 protein, and any one of the following applications: 1) improving the plant disease resistance; 2) cultivating plants with improved disease resistance; and 3) plant breeding. The OsFBX388 gene has the advantages that the OsFBX388 gene is down-regulated in expression, so that rice can spontaneously generate disease-like spots, the resistance to rice blast and bacterial leaf blight is enhanced, and the OsFBX388 gene has good application potential and application value in improvement of crop disease resistance.