64 results about "Trichoderma atroviride" patented technology

The invention relates to a trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and a preparation method thereofof the trichoderma atroviride preparation. A trichoderma strain Tr1208 is trichoderma atroviride and preserved in the CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No. 6828. According to The the trichoderma atroviride preparation takes, the trichoderma atroviride Tr1208 is used as an effective active ingredient and is prepared by being matched with an agriculturally acceptable carrier and a protective agent for the preparation; the bacteria content of the effective active ingredient is (1.0-30.0)*108 spores / gram; and the bacteria protectant content is 0.05%-1.8%. The preparation can have the effects of effectively preventing and controlling leek Botrytis, cucumber brown patches, cucumber powdery mildew, cucumber downy mildew, tomato early blight, cucumber type damping off, cucumber type blight and cucumber type root rot and promoting plant growth and characteristics of efficiency, wide antimicrobial spectrum, simple usage, low cost, no environmental pollution and plant micro ecological environment improvement.

The invention discloses an endophytic fungus in salvia miltiorrhiza bunge. The endophytic fungus is isolated and obtained from the living body of salvia miltiorrhiza bunge by adopting the endophytic fungus isolation and purification technology and is identified to be trichoderma atroviride through microbial taxonomy. The strain was collected in the China General Microbiological Culture Collection Center, with collection number being CGMCC No.4712.. Tanshinone compounds tanshinone I and tanshinone IIA are generated through liquid fermentation of the strain of the endophytic fungus in salvia miltiorrhiza bunge, so the endophytic fungus is an important microorganism for finding the new resources such as the tanshinone chemical components tanshinone I and tanshinone IIA.

The present invention relates to Trichoderma atroviride strains and their use as biological control agents or plant growth promoters. Methods and compositions for biological control of soil borne plant pathogens, and increasing plant yield using T. atroviride are also provided.

The invention relates to a trichoderma atroviride B-8-1-34 strain capable of highly yielding cellulose and application of the trichoderma atroviride B-8-1-34 strain in the preparation of the cellulose, belonging to the technical field of microorganisms. The trichoderma atroviride B-8-1-34 strain disclosed by the invention has been preserved in China General Microbiological Culture Collection Center on Aug.10, 2012, with the preservation number of CGMCC No.6390. The trichoderma atroviride B-8-1-34 disclosed by the invention can be used for producing the cellulose by making use of lignocellulose-containing industrial and agricultural wastes in combination of low-cost raw materials such as bran, maize slurry and soybean cake powder through liquid or solid fermentation, has the advantages of short fermentation period, high activity of produced cellulose, reasonable components and high lignocellulose hydrolysis, and provides a novel strain resource to solve the problems on high production cost, low saccharifying efficiency and the like caused by low enzyme activity and unreasonable enzyme components in the utilization of cellulose resources.

The invention provides crop growth promoting antagonistic trichoderma, in particular to Trichoderma atroviride H18-1-1 and TF28 with preservation numbers of CGMCC No.9774 and 9775 and aims to solve the problem that using agricultural chemicals for preventing and treating agricultural diseases is prone to causing environment pollution and agricultural chemical residues, jeopardizing an agricultural ecological system, killing beneficial microorganisms in the environment, improving drug resistance of phytopathogen and finally causing decline of prevention and treatment effects and even failure in prevention and treatment. The trichoderma not only can be applied to agricultural biological prevention and treatment but also can promote growth of crops such as wheat and prevent and treat diseases caused by rhizoctonia cerealis, fusarium culmorum, fusarium proliferatum and / or fusarium oxysporum and the like.

A method for enhancing growth of plants comprising contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant-Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Trichoderma system in a planting medium and to enhance plant growth. The Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11 Bab (ATCC accession number PTA 9709), and combinations thereof. Methods of enhancing resistance of plants to abiotic stress, increasing nitrogen use efficacy in plants, reducing nitrous oxide emissions in air, reducing leaching of nitrates into soil and water, and enhancing sequestration of carbon from air are disclosed.

The invention discloses a preparing method of an efficient straw decomposition agent, and belongs to the technical field of decomposition agent preparation. The preparing method includes the steps that thalli in rotted wood where mushroom grows are selected, enrichment culture is carried out on the thalli to obtain bacterium liquid, trichoderma atroviride microbial inoculum, bacillus subtilis microbial inoculum, saccharomyces cerevisiae microbial inoculum and bacillus thuringiensis microbial inoculum are activated on a slope, fermentation media are inoculated with the microbial inoculums and fermentation culture is performed respectively, all strain fermentation liquids are mixed to obtain a mixed fermentation liquid, the mixed fermentation liquid and the bacterium liquid are mixed and then concentrated, and an adsorbing carrier and the concentrated liquid are mixed to prepare the efficient decomposition agent. The prepared straw decomposition agent is high in decomposition speed and capable of completely degrading straw, promoting release of straw nutrient, improving the fertility improvement effect of soil with straw returning and improving the biological effect.

The invention relates to a trichoderma atroviride strain for producing myrosase and application thereof. The file number of the dark-green trichoderma atroviride strain for producing myrosase is CGMCC (China general microbiological culture collection) No.5609. During use, trichoderma atroviride is applied to soil containing brassica plant tissue as fumigated materials in a solid bactericide manner, pests in the soil are killed by biological fumigation, and pest diseases spread by the crop soil are prevented.

The invention relates to a Trichoderma atroviride-Bacillus amyloliquefaciens composite microbial agent for preventing and controlling solanaceous vegetable virosis and a preparation method thereof. The Trichoderma atroviride strain is Tr1208, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No. 6828. The Bacillus amyloliquefaciens strain is B1126, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No. 6827. The Trichoderma atroviride-Bacillus amyloliquefaciens composite microbial agent is prepared from Trichoderma atroviride Tr1208 and Bacillus amyloliquefaciens B1126 as active components, agriculturally acceptable carriers and a protection agent. The content of the active components is in a range of 5*10<9> to 2*10<10>cfu / g and the content of the microbial protection agent is in a range of 0.1-1%(w / w). The microbial agent can effectively preventing and controlling solanaceous vegetable viroses such as TMV, CMV and TYLCV, can promote plant growth, has high effects, can be used by a simple method, has a low cost, does not pollute the environment and can improve a plant micro-ecological environment.

The invention relates to the technical field of biopesticides, and particularly relates to application of trichoderma atroviride HB20111 to prevention and treatment of wheat stem rot and banded sclerotial blight. The trichoderma atroviride HB20111 is preserved in China General Microbiological Culture Collection Center on December 5, 2018, and the preservation number of the trichoderma atroviride HB20111 is CGMCC No. 16963. The invention belongs to the field of green biopesticides, and the use of the trichoderma atroviride HB20111 can correspondingly reduce the use of chemical pesticides and pesticide residues in agricultural products and has important significance for improving the quality of the agricultural products and is suitable for production and application of pollution-free green agricultural products.

The invention discloses a rapeseed endogenous trichoderma atroviride ReTv2 strain and a preparation method and application thereof. The trichoderma atroviride ReTv2 strain is separated from a rapeseed root tissue by the applicant, and the number CCTCC NO is M2014407. The ReTv2 strain is activated and cultured in a PDA culture medium at the temperature of 28 DEG C for 2-4d to obtain conidiospores, and the spores are inoculated into a PDB culture medium for enlarged culture for 6d, wherein the content of the conidiospores can achieve 1.0*10<9> / mL. The trichoderma atroviride ReTv2 strain disclosed by the invention is the rapeseed endogenous strain, can be successfully colonized at the root of rapeseed and has an effect on promoting growth of the rapeseed, a fermentation broth has an obvious effect on inhibiting germination of resting spores of pathogenic bacteria of rapeseed clubroot, and the control effect of the strain against the rapeseed clubroot achieves 66.4%; furthermore, the ReTv2 strain can be directly applied during seeding, is simple in an application method, can save labor force and further has the property of being safe to people and animals of biological pesticides.

A method for enhancing growth of plants comprising contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant-Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Trichoderma system in a planting medium and to enhance plant growth. The Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11 Bab (ATCC accession number PTA 9709), and combinations thereof. Methods of enhancing resistance of plants to abiotic stress, increasing nitrogen use efficacy in plants, reducing nitrous oxide emissions in air, reducing leaching of nitrates into soil and water, and enhancing sequestration of carbon from air are disclosed.

The invention discloses a co-culture method of trichoderma atroviride and bacillus subtilis and an application of a co-metabolite obtained by the method. According to the method, the trichoderma atroviride SG3403 and bacillus subtilis 22 strains are used as co-culture objects, the inhibition rate of a co-culture fermentation liquid against fusarium graminearum is used as a response value, and thefermentation conditions such as culture temperature, a rotation speed, a pH, a corn flour content, a molasses content, a yeast powder content and a KCl content are optimized by a response surface method to obtain the following best fermentation conditions: the culture temperature is 28 DEG C, the rotation speed is 214 rpm, the pH value is 6.8, the yeast powder content is 10 g / L, and the molasses content is 27.6 g / L. The co-culture fermentation liquid cultivated by the formula provided by the invention improves the resistance to the fusarium graminearum, a substance not found in a single culture is found in the co-culture fermentation liquid, a seed coating agent prepared from the substance promotes the growth of wheat, and a novel option is provided for the development of biological pesticides and biological seed coating agents.

The invention relates to a Trichoderma strain for antagonizing maize stem rot and banded sclerotial blight and application thereof, belonging to the field of biological control. The Trichoderma strain is Trichoderma atroviride SG3403 CGMCC No.6479. The invention also relates to application of the Trichoderma strain in preparing pesticides for controlling maize stem rot and banded sclerotial blight. The Trichoderma strain provided by the invention has the advantages of high growth speed and high spore production amount, the in-vitro standoff pathogen inhibition rates are respectively up to 71.91%, 74.77% and 73.11%, the enzyme activity of chitinase is 4.1452U, the enzyme activity of beta-1,3-glucanase is 0.5969U, and the enzyme activity of extracellular proteinase is 2.4545U. The strain provided by the invention can be used for producing biological pesticides for efficiently and quickly controlling maize stem rot and banded sclerotial blight.

The invention relates to a Trichoderma strain for antagonizing maize stem rot and banded sclerotial blight and application thereof, belonging to the field of biological control. The Trichoderma strain is Trichoderma atroviride SG3403 CGMCC No.6479. The invention also relates to application of the Trichoderma strain in preparing pesticides for controlling maize stem rot and banded sclerotial blight. The Trichoderma strain provided by the invention has the advantages of high growth speed and high spore production amount, the in-vitro standoff pathogen inhibition rates are respectively up to 71.91%, 74.77% and 73.11%, the enzyme activity of chitinase is 4.1452U, the enzyme activity of beta-1,3-glucanase is 0.5969U, and the enzyme activity of extracellular proteinase is 2.4545U. The strain provided by the invention can be used for producing biological pesticides for efficiently and quickly controlling maize stem rot and banded sclerotial blight.

The invention belongs to the technical field of soil improvement and particularly discloses a method for soil restoration by means of biological decomptosition of total straw returning in a crop rotation system. The straw returning technology relates to many factors such as proper temperature of the natural environment and sufficient water, microorganisms promote decomposition of mineralized nutrients, the straw carbon-nitrogen ratio is reduced, and the situation that straw fights for nitrogen elements with crops, and accordingly nitrogen elements must be additionally applied is avoided; cellulose and lignin start to decompose after microorganism bacteria are mixed with the straw; humic acid is used for intermediate guidance, trichoderma atroviride promotes conversion of rice and wheat and other cellulose so that soil can become permeable and loose, soil organic matter is added due to conversion of the cellulose, and soil fertility is improved to a certain extent after mineralization.

Trichoderma atroviride Ta-9 has the collection number of CGMCC No. 9698 and is collected at the CGMCC (China General Microbiological Culture Collection Center). The trichoderma atroviride Ta-9 disclosed by the invention can be used for preventing and controlling a variety of rice diseases as a biological pesticide. The trichoderma atroviride Ta-9 shows relatively strong superparasitism ability to rhizoctonia solani and ustilaginoidea virens. The invention further relates to trichoderma atroviride Ta-9 high-yield chlamydospore and conidiospore liquid fermentation technologies. After fermentation liquor of the trichoderma atroviride Ta-9 is diluted by 50 times, the diluted fermentation liquor has relatively good prevention and control action on both rice sheath blight disease caused by rhizoctonia solani and green smut caused by ustilaginoidea virens.

The invention discloses an application of improved ATMT (Agrobacterium Tumefaciens-Mediated Transformation) to construction of trichoderma atroviride T23 deltaCrel. The application is characterized in that the genomic DNA of trichoderma atroviride T23 is taken as a template, PCR (Polymerase Chain Reaction) amplification is carried out on the Crel gene sequence, and thus upstream and downstream flanking sequences of the Crel gene are obtained; then leading in respectively to knock out upstream and downstream of plasmid pC1300qh, so as to form recombinant plasmid pC1300qh-deltaCrel; converting escherichia coli competence of the recombinant plasmid pC1300qh-deltaCrel, performing verification, screening positive clone, extracting recombinant plasmid pC1300qh-deltaCrel, leading in agrobacterium tumefaciens AGL1, and carrying out improved ATMT. By using the improved ATMT technique, a trichoderma atroviride carbon metabolism inhibiting factor CRE1-knocked-out mutant strain is successfully constructed; the T23 deltaCrel bacterial strain has the functions of promoting secretion and activity of degrading enzyme of cell walls of trichoderma spp., and indirectly improving the function of trichoderma spp. to antagonize pathogenic bacteria.

The invention relates to a soil improvement agent trichoderma atroviride DQ1 composite powder TLB, and applications thereof. The soil improvement agent trichoderma atroviride DQ1 composite powder TLB is prepared via following steps: 1, PDA slant culture-medium is inoculated with trichoderma atroviride DQ1 so as to obtain a spore liquid, and sterilized PD culture medium is inoculated with the spore liquid for culturing; 2, a pore suspension liquid is prepared from wheat bran, diatomite, rice husk, and water, and is taken as a solid shallow tray fermentation seed liquid; 3, a solid shallow tray fermentation medium is prepared from wheat bran, diatomite, streptomycin, and sterile water at a mass ratio of 50:25:1:50, is stirred, and is sterilized, the sterilized solid shallow tray fermentation medium is mixed with the solid shallow tray fermentation seed liquid, culturing is carried out, an obtained shallow tray fermentation product is subjected to air drying and smashing so as to obtain trichoderma atroviride DQ1 spore powder; and 4, the soil improvement agent trichoderma atroviride DQ1 composite powder TLB is prepared. The soil improvement agent trichoderma atroviride DQ1 composite powder TLB is capable of preventing and treating vegetable soil borne diseases, and is harmless for human and animals, and no environment pollution is caused.

The invention relates to a trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and a preparation method thereofof the trichoderma atroviride preparation. A trichoderma strain Tr1208 is trichoderma atroviride and preserved in the CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No. 6828. According to The the trichoderma atroviride preparation takes, the trichoderma atroviride Tr1208 is used as an effective active ingredient and is prepared by being matched with an agriculturally acceptable carrier and a protective agent for the preparation; the bacteria content of the effective active ingredient is (1.0-30.0)*108 spores / gram; and the bacteria protectant content is 0.05%-1.8%. The preparation can have the effects of effectively preventing and controlling leek Botrytis, cucumber brown patches, cucumber powdery mildew, cucumber downy mildew, tomato early blight, cucumber type damping off, cucumber type blight and cucumber type root rot and promoting plant growth and characteristics of efficiency, wide antimicrobial spectrum, simple usage, low cost, no environmental pollution and plant micro ecological environment improvement.

The invention discloses a Trichoderma atroviride strain capable of producing high-temperature-resistant feruloyl esterase and high-temperature-resistant cellulase and application thereof. The collection number of the Trichoderma atroviride AWS26 strain is CGMCC No.8673. The Trichoderma atroviride strain has the advantages of low nutritional requirements, high reproduction speed and short fermentation period, and the fermentation products can be easily extracted and separated. The Trichoderma atroviride strain can be fermented to obtain the feruloyl esterase-cellulase composite enzyme. The composite enzyme has the advantages of high temperature resistance and high acid / alkali stability.

The present invention relates to Trichoderma atroviride strains and their use as biological control agents or plant growth promoters. Methods and compositions for biological control of soil borne plant pathogens, and increasing plant yield using T. atroviride are also provided.

The invention discloses a method for preparing heat-resistant acid-resistant feruloyl esterase by using trichoderma atroviride. According to the method, trichoderma atroviride serves as a production strain, heat-resistant feruloyl esterase is prepared through the processing steps of preparing fermenting strains, carrying out triangular flask solid-state fermentation, carrying out koji tray solid-state fermentation, preparing a crude enzyme preparation and purifying the crude enzyme preparation, and the obtained enzyme preparation is high in enzyme activity, good in acid resistance and high in heat resistance; the preparation method is short in fermentation cycle, low in energy consumption, low in water consumption, low in environmental pollution degree and high in specific yield and has excellent industrialization application prospects.

The invention provides an artemisia apiacea seedling cultivating method which comprises the following steps: holing rhizosphere soil of artemisia apiacea seedlings, irrigating trichoderma atroviride K fertilizer and then covering soil. The invention provides trichoderma atroviride K fertilizer applied to cultivation of artemisia apiacea seedlings, and fills a blank of the artemisia apiacea planting field. Meanwhile, the growth and development of the artemisia apiacea seedlings can be effectively promoted by adopting the trichoderma atroviride K fertilizer, and the trichoderma atroviride K fertilizer is greener and more environment-friendly compared with the fertilizer.

A kind of Trichoderma atroviride TA‑9, which has been preserved in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.9698. The Trichoderma atroviride TA-9 of the present invention can be used as a biological pesticide to prevent and control various rice diseases. Trichoderma dark green TA‑9 showed strong hyperparasitic ability against Rhizoctonia solani and Ustilaginoidea virens. The present invention also relates to the high-yield chlamydospore and conidia liquid fermentation technology of Trichoderma dark viridans TA-9, after its fermented liquid is diluted 50 times, it can control rice sheath blight caused by Rhizoctonia solani and rice sheath blight caused by Chlorosclerotinum oryzae. Aspergillus has a good control effect.

The invention discloses a compound Trichodermanin B with the following structural formula, which is separated and prepared from a cephalotaxus fortunei endophytic fungi Trichoderma atroviride as a source. The molecular formula of the compound is C15H28O3 and the molecular weight is 256. The invention also discloses a preparation method for the compound. In vivo experiments show that the compound has certain fungi resistant, anti-virus and tumor resistant activities. The invention lays a foundation for the development of new active medicaments.

The present technology relates generally to compositions, methods and systems entailing one or more microbial species or microbe derivatives therefrom or combinations of a microbial species pins at least one microbe derivative, in combination with one or more agricultural chemicals such as a fungicide, an insecticide, a nematicide, a bacteriocide, an herbicide or a mixture thereof for application to crops for enhanced growth and performance. The microbial species are preferably selected from the group consisting of Trichoderma virens, Trichoderma atroviride, Trichoderma afroharzianum, Trichoderma strain K1, Trichoderma strain K2, Trichoderma strain K3: Trichoderma strain K4, Trichoderma strain K5 and mixtures thereof. The microbe derivatives are preferably microbial metabolites selected from the group consisting of 6-pentyl pyrone, harzianic acid, hydtra 1, harzinolide, 1-octene-3-ol and mixtures thereof. The compositions are preferably applied to seeds of the crop or by any of the common methods onto the crop in the field.

The invention relates to a trichoderma atroviride dispersible oil suspending agent containing indolebutyric acid and amino-oligosaccharin. The trichoderma atroviride dispersible oil suspending agent comprises the following components in parts by weight: 0.5-30 parts of trichoderma atroviride spore powder, 0.5-5 parts of indolebutyric acid, 0.5-10 parts of the amino-oligosaccharin, 3.3-50.6 parts of an auxiliary agent and 20-85 parts of vegetable oil. According to the trichoderma atroviride dispersible oil suspending agent containing the indolebutyric acid and the amino-oligosaccharin, the indolebutyric acid and the amino-oligosaccharin which have the effects of promoting rooting, inducing crops to generate resistance and improving the immunity of the crops are added while the trichoderma atroviride is used for preventing and treating nematodes, so the harm of the nematodes can be reduced; and meanwhile, the indolebutyric acid enables injured root systems to rapidly grow new roots, and the amino-oligosaccharin induces the crops to generate resistance and can provide certain nutrition for the trichoderma atroviride powder and the crops. The invention also relates to a preparation method and application of the trichoderma atroviride dispersible oil suspending agent containing the indolebutyric acid and the amino-oligosaccharin.

The invention discloses an escherichia coli engineering bacterium containing the gene of Epl1 (eliciting plant response protein1) and a construction method of the escherichia coli engineering bacterium.. The escherichia coli engineering bacterium BL21-Epl1 containing the gene of Epl1 is gram negative brevibacterium and facultative anaerobe, and the bacterial colony of the escherichia coli engineering bacterium BL21-Epl1 is circular, smooth, colorless and transparent. The construction method comprises the following steps: I, extracting a mycelium cDNA (Complementary Deoxyribonucleic Acid) of trichoderma atroviride; II, constructing a prokaryotic expression vector Pgex-EPl1; and III, converting escherichia coli to obtain the escherichia coli engineering bacterium. Under the condition that the fermenting property is not affected, after being induced by a recombinant protein rEPl1, the escherichia coli engineering bacterium provided by the invention remarkably excites the expression of genes related to signal transfer paths of salicylic acid, jasmonic acid and auxin signals of Shanxinyang. The production process of the escherichia coli engineering bacterium is simple and low in cost. The escherichia coli engineering bacterium containing the gene of Epl1 (eliciting plant response protein1) and the construction method thereof are applied to the agricultural field.

The invention discloses trichoderma atroviride and application thereof in inhibiting pathogenic bacteria of ginseng, and relates to the technical field of ginseng cultivation in farmland. Trichoderma atroviride is separated and screened from soil, and a ginseng pathogenic bacterium inhibition test shows that the screened trichoderma atroviride can effectively inhibit the growth of various ginseng pathogenic bacteria. That is to say, the trichoderma atroviride provided by the invention has a certain prevention and treatment effect on diseases and has a good biocontrol application prospect. In addition, the trichoderma atroviride has a remarkable growth promoting effect on growth of roots, stems and leaves of ginseng. After the trichoderma atroviride is applied, the existing ginseng cultivation environment can be improved to a great extent, so that the yield and quality of the ginseng can be improved. The trichoderma atroviride can protect the population structure of soil microorganisms.