10 results about "Trichoderma atroviride" patented technology

This invention discloses a method for controlling root rot of Panax notoginseng based on a fungal synthetic microbial community and its application, belonging to the field of plant disease biological control technology. The method involves screening six fungal strains from the rhizosphere soil of healthy Panax notoginseng plants that have survived for three years in continuously cropped fields. These strains exhibit highly efficient antagonistic activity (inhibition rate ≥70%) against three pathogens of the genus *Fusarium*. A synthetic microbial community containing *Trichoderma atroviride*, *Trichoderma hamatum*, *Trichoderma petersenii*, *Trichoderma neokoningii*, *Trichoderma virilente*, and *Phanerochaete concrescens* was constructed at a viable count ratio of 1:350:590:14:30:755, and prepared to a spore concentration of 10... 8 -10 9 Capsules containing 10⁸ CFU / g are used. Before planting, apply the fertilizer to the bottom of the planting hole. After emergence, apply the fertilizer using a fertilizer applicator within a 5cm radius around the roots, 20-30g per plant (based on 10⁸ CFU / g). Apply once a month from 4-6 months after emergence, for three consecutive applications. During the application period, maintain soil moisture at 60%-70% and a temperature of 13-20℃. This method can reduce the incidence of root rot in Panax notoginseng to below 10.60%, while increasing yield per plant by 6% and increasing total saponin content by ≥20%, combining prevention, growth promotion, and environmental safety.

The invention discloses a compound microorganism for preventing and treating chestnut fruit rot and application of the compound microorganism. The compound microorganism is composed of trichoderma atroviride, bacillus siamensis and bacillus velezensis, and is constructed by directionally screening strains with broad-spectrum inhibition effect on seven core pathogenic bacteria from Chinese chestnut fruit endophytes and verifying compatibility. The method has the advantages that the compound flora synergistically prevents and controls diseases through direct antagonism and induction of plant system resistance, and the defensive enzyme activity and the content of total polyphenol and total flavone of Chinese chestnuts are remarkably improved; according to the compound microorganism, the indoor control effect reaches 90% (the morbidity is reduced), the diseased fruit rates of Chinese chestnut fruits treated in a chestnut garden are reduced by 60% and 42.86% respectively after the Chinese chestnut fruits are stored for 30 days and 120 days, the effectiveness and the stability are obviously higher than those of a single strain, the compound microorganism can be introduced in the flowering period or sprayed in the storage period, use is convenient and flexible, and an efficient and stable biocontrol strategy is provided for green prevention and control of the Chinese chestnut fruit rot.

The invention provides application of trichoderma atroviride in relieving continuous cropping obstacles of ginseng or salvia miltiorrhiza, and relates to the technical field of crop cultivation. Through deep research and creative labor, the trichoderma atroviride Tri802 can effectively relieve soil chemical property deterioration and soil microorganism change after continuous cropping of the ginseng or the salvia miltiorrhiza, further can improve the soil chemical property after continuous cropping of the ginseng or the salvia miltiorrhiza, increases the relative abundance of beneficial bacteria in the soil, inhibits the relative abundance of harmful bacteria, and improves the yield of the ginseng or the salvia miltiorrhiza. Meanwhile, the content of effective components of ginseng or salvia miltiorrhiza can be increased, and the quality of continuous cropping ginseng or salvia miltiorrhiza is improved.

The invention discloses a trichoderma atroviride engineering strain for expressing dsRNA (double-stranded ribonucleic acid) as well as a preparation method and application of the trichoderma atroviride engineering strain, the trichoderma atroviride engineering strain is trichoderma atroviride which is integrated with a dsRNA expression box expressed by an interference target gene BdSpt7, the dsRNA expression box is as shown in SEQ ID No.1, and the trichoderma atroviride is trichoderma atroviride T11 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41710. The preparation method of the trichoderma atroviride engineering strain comprises the following steps: constructing a dsRNA expression framework, constructing a recombinant expression vector and transforming protoplast. The trichoderma atroviride engineering strain can stably express a dsRNA expression cassette of a target gene BdSpt7 so as to play a role in preventing and treating physalospora piricola, is stable in performance and high in delivery efficiency, and can be used for preparing a biocontrol preparation and applied to prevention and treatment of physalospora piricola.

The application discloses a kind of for preventing and treating chestnut fruit rot disease complex microorganism and its application.The application complex microorganism is composed of Trichoderma atroviride and Bacillus siamensis, Bacillus velezensis, by from chestnut fruit endophyte directional screening to seven kinds of core pathogenic bacteria have broad-spectrum inhibitory effect strain, is constructed after compatibility verification.It is superior in that: complex microbial population is synergistically controlled disease by direct antagonism and induced plant system resistance, significantly improves chestnut defense enzyme activity and total polyphenol, total flavone content;Indoor control efficiency reaches 90% (reduces the incidence), chestnut fruit in chestnut orchard treatment is reduced 60% and 42.86% respectively in 30 days and 120 days, effective and stability are obviously stronger than single strain, and the application complex microorganism can be introduced by flowering period or sprayed in storage period, and is convenient and flexible, provides efficient, stable biocontrol strategy for chestnut fruit rot green control.

The invention belongs to the technical field of microorganisms, and relates to trichoderma atroviride SH-L1 and application thereof. The preservation number of the trichoderma atroviride SH-L1 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 41751. The invention also provides a microbial agent, which comprises trichoderma atroviride SH-L1 and / or fermentation liquor or metabolite of the trichoderma atroviride SH-L1. The trichoderma atroviride SH-L1 obtained by separation and screening can inhibit the growth of aspergillus flavus, inhibit the generation of aflatoxin or reduce the content of aflatoxin, promote the growth of peanut seedlings, improve the peanut yield and significantly inhibit peanut soil-borne bacteria, and has the advantages of being green, environmentally friendly, high in specificity, environmentally friendly and the like; peanut yield and quality safety can be guaranteed, and healthy development of the peanut industry is promoted.

The invention relates to the technical field of agricultural microbial preparations, and discloses a sweet potato root rot antagonistic fungus preparation as well as a preparation method and an application method thereof. The preparation consists of a trichoderma harzianum and trichoderma atroviride compound strain, a modified charcoal-humic acid organic carrier, a chitosan-sodium alginate microcapsule wall material, a dextrin-trehalose stabilizer and a sodium salicylate / methyl jasmonate synergistic factor, and is prepared by adopting a solid state fermentation process, a fluidized bed drying process, a microcapsule embedding process and a fluidized bed granulation process. The field control effect reaches 78%-92%, the lasting period is 3-4 months, and the pesticide composition has no inhibition effect on beneficial microorganisms in soil.

This invention discloses a biocontrol compound microbial strain YC and its application, relating to the field of microbial technology. The biocontrol compound microbial strain YC is composed of *Trichoderma harzianum* YP2 and *Trichoderma viride* CK1A. *Trichoderma harzianum* YP2 was deposited on May 22, 2025, at the China General Microbiological Culture Collection Center, and is classified as follows: Trichoderma afroharzianum YP2, with accession number CGMCC NO. 41947; the described *Trichoderma viride* CK1A was deposited on May 22, 2025, at the China General Microbiological Culture Collection Center, and classified as... Trichoderma atroviride CK1A, with accession number CGMCC NO. 41948. The biocontrol compound microbial system YC described herein is used to control maize stalk rot. The biocontrol compound microbial system YC of this invention exhibits stable efficacy and strong antibacterial ability, opening up new avenues for the biological control of maize stalk rot in my country.

The invention belongs to the technical field of microorganisms, and discloses trichoderma atroviride, a biocontrol microbial agent and application of the trichoderma atroviride, the trichoderma atroviride is named as trichoderma atroviride T11, the preservation number of the trichoderma atroviride T11 is CGMCC No.41710, the trichoderma atroviride is preserved in China General Microbiological Culture Collection Center on December 11, 2024, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The active ingredient of the biocontrol inoculant is the trichoderma atroviride. The trichoderma atroviride T11 has an inhibiting effect on various plant disease pathogenic bacteria, also has a growth promoting effect on fruit trees, can be used for preparing a biocontrol microbial inoculum and applied to prevention and treatment of fruit and vegetable diseases, can also be used as an active ingredient for preparing a plant growth promoting agent, and is stable in effect and environment-friendly.

The invention discloses trichoderma atroviride and biocontrol application thereof, and belongs to the technical field of biological control. The trichoderma atroviride is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 3, 2025, the preservation number is CGMCC No.41906, and the trichoderma atroviride is classified and named as the trichoderma atroviride. The trichoderma atroviride has the advantages that the trichoderma atroviride can be used for preparing the trichoderma atroviride; the trichoderma atroviride strain disclosed by the invention has remarkable bacteriostatic activity on Cang-source colletotrichum, fruit-growing colletotrichum, botryosphaeria dothidea, fusarium proliferatum and fusarium oxysporum, and fermentation liquor of the trichoderma atroviride strain can be used for effectively preventing and treating eucalyptus blight and cunninghamia lanceolata anthracnose. The strain can be used as an efficient biocontrol functional strain, and has a wide industrial application prospect in the field of green biological control of forest tree diseases.