167 results about "Aspergillus flavus" patented technology

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The invention relates to bacillus velezensis and application thereof in prevention and treatment of wheat leaf blight and inhibition of plant pathogenic fungi, and belongs to the field of biological prevention and treatment. The bacillus velezensis HXYT7 is separated and screened from tobacco leaves growing in a tobacco plantation (the longitude is 113.73 and the latitude is 33.91) in Xiangchang County in elm city, Henan province, has a remarkable inhibition effect on mophila, fusarium moniliforme, fusarium graminearum, rhizoctonia cerealis, aspergillus flavus and aspergillus niger, can damage the hypha structure of the mophila, and can be used for preparing the bacillus velezensis HXYT7. The bacillus subtilis can inhibit germination of spores of the helminthosporium, has an obvious prevention and treatment effect on the wheat leaf blight caused by the helminthosporium, and provides a new microbial resource for prevention and treatment of the wheat leaf blight.

The invention discloses a method for detecting aspergillus flavus and aflatoxin B1 of crops based on an infrared spectrum. The method comprises the steps of crop sample material collection, rice multi-strain mould infection, spectrum collection, crop sample aflatoxin B1 determination, spectrum pretreatment, correction model construction and model verification. The invention aims at detecting the content of aspergillus flavus infection and toxin B1 thereof in crops by combining a near-infrared spectrometer and a stoichiometric method. The method has the advantages of being simple and easy to implement, high in analysis speed, high in detection precision, low in analysis cost, free of any pollution to the environment and the like, samples do not need any pretreatment, and the method has important practical value for guaranteeing the quality safety of agricultural products and reducing the carcinogenic risk of people.

The invention discloses a new application of aspergillus flavus in prevention and treatment of epidemic blight of Eupin, and relates to the technical field of microbial prevention and treatment, the aspergillus flavus is CZB-13, the taxonomic name of the aspergillus flavus is Aspergillus flavus, the strain is preserved in China Center for Type Culture Collection on July 8, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20241494. The aspergillus flavus CZB-13 provided by the invention has a remarkable inhibition effect on the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight of the epidemic blight. The method is also of great significance in protecting the ecological environment and promoting green prevention and control of epidemic diseases of the elaeagnus plants.

The invention discloses streptomyces allogeri and an application thereof in biological prevention and control of toxin-producing fungi. In particular to streptomyces allorgeus ZxBac193 CGMCC (China General Microbiological Culture Collection Center) No.32341 and an application thereof. The streptomyces allorgeus ZxBac193 shows a strong antagonistic effect on toxin-producing fungi such as aspergillus flavus, aspergillus ochraceus, fusarium graminearum, fusarium oxysporum, penicillium citrinum and penicillium islanigerum; the compound can inhibit the growth of aflatoxin producing fungi, ochratoxin producing fungi, deoxynivalenol producing fungi, zearalenone toxin producing fungi, fumonisins producing fungi, citrinin producing fungi and island penicillin producing fungi, and can be prepared into various biological control products aiming at mycotoxins, such as biopesticides, antifungal agents and the like. The compound is used for inhibiting toxic fungi in the environment and preventing or relieving mycotoxin pollution, and has good development and application prospects in the fields of prevention and treatment of fungal pollution of food crops and the like.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention discloses a mycotoxin degrading bacterium (Lactobacillus plantarum) R4-30 and application thereof, the strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M2018437, and the strain is obtained by screening in Guangxi sugarcane tail leaf silage. The strain can inhibit the growth of aspergillus flavus and fusarium graminearum, so that the production of mycotoxin is reduced, and the mycotoxin can be degraded. The aflatoxin B1 degradation rate of the strain is as high as 91.21% when the bacterial concentration is 12 * 10 < 8 > cfu / ml, the pH value is 2 and the culture time is 16 days; when the bacterium concentration is 3 * 10 < 8 > cfu / ml, the pH value is 6 and the culture time is 12 days, the zearalenone degradation rate is as high as 94.56%; when the bacterial concentration is 12 * 10 < 8 > cfu / ml, the pH value is 2 and the culture time is 16 days, the degradation efficiency of AFB1 and ZEN in the mango peel fermented feed is remarkably improved, the degradation rates of AFB1 and ZEN under the conditions reach 91.2% and 58.16% respectively, the nutritional quality is high, the fermentation quality is good, chemical additives are not needed, and the method is suitable for green detoxification treatment of agricultural products and feed.

The invention belongs to the field of molecular genetic breeding, and particularly relates to a KASP molecular marker method related to peanut aspergillus flavus seed kernel infection resistance and application of the KASP molecular marker method. Through whole genome association analysis, the SNP locus is located at the 133198521th bp position of the peanut B06 chromosome (the reference genome is peanut), the nucleotide sequence polymorphism is C / A (the antisense strand is G / T), and the SNP locus is named as qARIB06. Based on the SNP site, KASP molecular marker primer sequences (SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3) are designed and developed, and the KASP molecular marker primer sequences are used for rapidly and accurately identifying the genotype of a peanut sample.

The invention discloses a construction method of a mutated uricase prokaryotic expression engineering bacterium, and belongs to the technical field of gene engineering. The uricase encoding gene UOX2 is obtained by mutating four active sites of the uricase encoding gene derived from aspergillus flavus, a recombinant expression vector of the UOX2 encoding gene is constructed and is introduced into escherichia coli BL21, and the constructed prokaryotic expression engineering bacterium for producing uricase is suitable for industrial batch production and has a broad application prospect. And the specific activity of the uricase protein reaches 18.3 U / mg, so that a good foundation is laid for large-scale production of uricase.

The invention discloses a Trichoderma reesei strain and an application of the Trichoderma reesei strain in degradation of mycotoxin and inhibition of toxin-producing bacteria. The Trichoderma reesei strain is a Trichoderma reesei strain GG-T40, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20231843. The strain can synchronously and efficiently degrade eight kinds of mycotoxins such as AFB1, ZEN, T-2, ST, OTA, AOH, AME and TeA, and after the strain and the mycotoxins are co-cultured for 7 days, the removal rates of the eight kinds of toxins are all larger than or equal to 60%. The strain has strong bacteriostasis and toxin production inhibition effects, the toxin production inhibition rates of the strain to aspergillus flavus and fusarium graminearum are 62.5% and 74.4% respectively, and the strain can be applied to degradation of mycotoxin in agricultural products and food and inhibition of mildew and toxin production of the agricultural products and food.

The invention relates to the field of microorganisms, in particular to bacillus subtilis, a fungicide and application of the fungicide. The invention discloses a bacillus subtilis strain, which is classified and named as bacillus subtilis, has a strain number of GXGD-YZ-2483, is preserved in Guangdong Microbial Culture Collection Center on March 13, 2025, and has a preservation number of GDMCC No: 66014. The bacillus subtilis can tolerate the high temperature of 70-90 DEG C, has excellent self-heat production performance, and can produce lignin degrading enzymes (laccase, manganese peroxidase and / or lignin peroxidase) and inhibit the growth of pathogenic bacteria (escherichia coli, salmonella, clostridium perfringens and / or aspergillus flavus). When the bacillus subtilis or the microbial inoculum prepared from the bacillus subtilis is used for biologically composting organic wastes, a lignin barrier can be efficiently cracked by secreting a lignin degrading enzyme system, lignin degradation, nitrogen retention and pathogenic bacteria inactivation can be synchronously realized by virtue of high temperature resistance, self-heat production characteristics and enzyme activity stability, the composting period is shortened, and the yield of the organic wastes is improved. The humification quality is obviously improved.

The invention discloses bacillus subtilis K6 which is preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus subtilis K6 is CCTCC (China Center for Type Culture Collection) NO: 20231096. The invention further discloses application of the bacillus subtilis K6 in detoxification of the corn germ meal, the bacillus subtilis K6 is used for fermenting and detoxifying polluted corn germ meal, the degradation rate of zearalenone in the corn germ meal reaches 95% or above, the degradation rate of aspergillus flavus B1 in the corn germ meal reaches 90% or above, toxic substances can be effectively degraded, the detoxification activity is high, and the bacillus subtilis K6 can be applied to detoxification of the corn germ meal. And the method has good tolerance to high temperature and low pH, has stable detoxification capability, and can remove recessive zearalenone toxin contained in the cake meal at the same time. The utilization value of the corn germ meal can be improved, the nutrient components of the corn germ meal can be improved, and a good technical foundation is laid for deep processing of the corn germ meal.

The invention relates to a microbial agent based on a phosphate tailing storage yard and application of the microbial agent, the microbial agent based on the phosphate tailing storage yard comprises a talaromyces new species Talaromyces coriarcola KY-QH135, which is preserved in China Center for Type Culture Collection in Wuhan University with the number of CCTCC M 20252753, and the microbial agent based on the phosphate tailing storage yard is preserved in China Center for Type Culture Collection in Wuhan University with the number of CCTCC M 20252753. The invention relates to the field of biotechnology, and in particular relates to a biotechnology which is selected from one or more of Aspergillus tubingensis, Aspergillus flavus, Aspergillus oryzae, Aspergillus bombycis and Aspergillus niger, as well as one or more of Aspergillus tubingensis, Aspergillus flavus, Aspergillus oryzae, Aspergillus bombycis and Aspergillus niger.

The invention discloses application of a licoflavone compound in resisting food-borne pathogenic bacteria and dairy cow mastitis pathogenic bacteria. The licoflavone compound has the advantages that the licoflavone compound can be used for treating food-borne bacterial diseases caused by food-borne bacteria such as escherichia coli, bacillus cereus, listeria monocytogenes and salmonella enteritidis; food-borne fungal diseases caused by food-borne fungi such as aspergillus flavus, penicillium expansum and monilinia coerulea; the licochalcone A shows certain inhibitory activity on dairy cow mastitis pathogenic bacteria caused by streptococcus agalactiae, streptococcus dysgalactiae, escherichia coli and staphylococcus aureus, and particularly has an obvious inhibitory effect on food-borne pathogenic bacteria and dairy cow mastitis pathogenic bacteria by three licochalcone A with different contents. The compound disclosed by the invention is simple in structure and relatively high in safety, and is expected to be developed into a novel antibacterial drug.

The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus capable of inhibiting growth of monascus and application of the plant lactobacillus. The preservation number of the plant lactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.34851. According to the present invention, the plant lactobacillus CCNH295 has strong bacterial inhibition ability on common soybean meal contaminating molds, such as aspergillus flavus, aspergillus ochraceus, fusarium moniliforme and penicillium; the plant lactobacillus CCNH295 has excellent bacteriostatic ability to monascus, and when the plant lactobacillus CCNH295 is used for fermentation production of soybean meal, growth of monascus can be remarkably inhibited, so that mildewing of the fermented soybean meal is effectively inhibited, and the prepared fermented soybean meal does not mildew after being placed at 37 DEG C for 6 months; besides, the fermented soybean meal produced by solid state fermentation of the plant lactobacillus CCNH295 is used as a feed raw material, so that the stability of the feed can be improved, and the palatability of the feed can be improved.

The invention discloses a urate oxidase mutant (rAfUOX) with trypsin and chymotrypsin tolerance and application of the urate oxidase mutant (rAfUOX). The invention provides a urate oxidase mutant with trypsin and chymotrypsin tolerance by modifying a key amino acid residue in a wild type aspergillus flavus urate oxidase molecule through a protein engineering technology. The activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after trypsin digestion is increased by 244% compared with the activity half-life period of the wild type rAfUOX, and the activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after chymotrypsin digestion is increased by 191% compared with the activity half-life period of the wild type rAfUOX. Other enzymatic properties are basically consistent with those of the wild type rAfUOX.

The invention discloses a strain of bradyrhizobium guangzhouense APHBXY-23-1 with functions of efficiently promoting growth and strongly inhibiting aspergillus flavus and application of the strain of bradyrhizobium guangzhouense APHBXY-23-1. The preservation number of the strain of bradyrhizobium guangzhouense APHBXY-23-1 is CCTCC (China Center for Type Culture Collection) M 20253048. The invention not only retains the core functions of the strain as rhizobium for efficient symbiotic nitrogen fixation and crop growth promotion, but also reveals that the strain can significantly inhibit the growth of aspergillus flavus by releasing a volatile substance dimethylbutyric acid for the first time. The discovery breaks through the technical limitation that the traditional rhizobium only has a growth promoting function and lacks a biocontrol effect, the two agricultural microorganism core functions of nitrogen fixation and growth promotion and biocontrol bacteriostasis are endogenously integrated into the same microorganism carrier for the first time, and a novel rhizobium resource with double functions is successfully constructed; a new species of growth-promoting rhizobium with a biocontrol function is created, and the growth-promoting rhizobium has important innovative value in theory and application levels.

The application provides an Aspergillus flavus and an enzyme production method and application thereof, and belongs to the technical field of microorganisms.The Aspergillus flavus (Aspergillus flavus) HNZY01 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.42147.The Aspergillus flavus HNZY01 has the ability of high-yield cellulase, can efficiently and synergistically decompose key structural components such as cellulose, hemicellulose and lignin in tobacco stems, and can also increase the content of reducing sugar in the tobacco stems, so that the Aspergillus flavus HNZY01 can be applied in the degradation of the tobacco stems and the smoking safety of cigarettes can be improved.

The invention discloses a litsea cubeba essential oil preparation. The preparation is prepared by uniformly mixing a chitosan quaternary ammonium salt solution with the mass percent concentration of 1% and a litsea cubeba essential oil ethanol mixed solution according to the volume ratio of 1: 4, the volume ratio of the litsea cubeba essential oil to the absolute ethyl alcohol in the litsea cubeba essential oil and ethyl alcohol mixed solution is 2: 3. The prepared litsea cubeba essential oil preparation is good in dispersity and stability, the short-term and long-term bacteriostatic effects on aspergillus flavus both reach 100%, and the litsea cubeba essential oil preparation is obviously better than litsea cubeba essential oil or chitosan quaternary ammonium salt independently used, is also better than chitosan quaternary ammonium salt-litsea cubeba essential oil preparations of other formulas, is better than common mildew preventives in the market, and is suitable for industrial production. The preparation method is simple, non-toxic and harmless, does not pollute the environment, is low in production cost, is used for preventing or inhibiting the growth of the aspergillus flavus, and can reduce the damage of the aspergillus flavus to grains, nuts or wooden living goods and the like.

The invention discloses a fructus cinnamoii essential oil composition with a bacteriostatic effect as well as a preparation method and application thereof, and relates to the technical field of medicines. The invention discloses a fructus cinnamoii essential oil composition with a bacteriostatic effect, and the fructus cinnamoii essential oil composition with the bacteriostatic effect comprises the following components: 1-3.5 parts by mass of fructus cinnamoii essential oil, 1 part by mass of a wall material composed of gelatin and Arabic gum in a mass ratio of 0.4: 0.6-0.7: 0.3, and 0-4 parts by volume of a formaldehyde solution, the preparation method of the composition is simple, and the composition has a remarkable inhibiting effect on aspergillus flavus, penicillium expansum and fusarium oxysporum, and has a good application prospect. The invention can be used for preparing fungus inhibitors. The fructus cinnamoii essential oil microcapsule prepared from the fructus cinnamoii composition provided by the invention is stable in physicochemical property and remarkable in antibacterial effect, the preparation method is simple, and the fructus cinnamoii essential oil microcapsule has a wide market application prospect.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

The invention relates to a preparation method of a mould-corrosion-resistant ABS electromagnetic protective coating in a humid environment. The preparation method is characterized by comprising the following steps: pretreatment of an ABS matrix, preparation of an electromagnetic shielding layer, preparation of a mould-corrosion-resistant layer and preparation of a hydrophobic layer. In the ABS substrate pretreatment, a diamond free abrasive mechanical polishing method is adopted to remove an oxide layer not exceeding 10 microns on the surface, in the electromagnetic shielding layer preparation, a compact Cu-plated layer is reduced on the surface of the substrate by using an Ag nanoparticle-doped chemical copper plating process, and in the mold corrosion resistant layer preparation, a compact Ni-plated layer is prepared by using a nickel electroplating process on the surface of the electromagnetic shielding layer. And the hydrophobic layer is prepared by injecting a graphene oxide-doped polyurethane layer on the surface of the mould corrosion resistant layer through an injection molding process. According to the invention, 80-100 dB attenuation of broadband (1-18 GHz) electromagnetic waves is realized through the 10-13 [mu] m ultrathin composite coating, the growth of aspergillus niger, aspergillus flavus and other moulds can be inhibited, and the application prospect is wide.

The invention discloses sinorhizium xinjiangense APXJHT24-1 which has the functions of promoting growth and inhibiting aspergillus flavus, and the preservation number of the sinorhizium xinjiangense APXJHT24-1 is CCTCC (China Center for Type Culture Collection) M 2026229. According to the invention, the integration of two major functions of'generation of a characteristic antibacterial volatile matter of dimethyl disulfide 'and'efficient symbiotic nitrogen fixation' on a single strain is realized in Sinkiang Sinkiang rhizobium for the first time. The dimethyl disulfide released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in the aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.

The invention discloses a uricase mutant. The uricase sequence from aspergillus flavus and the uricase sequence from candida are taken as templates, multiple rounds of random mutation are carried out, sequencing verifies that the key amino acid site of the catalytic center is mutated, and the catalytic activity is enhanced. In-vivo and in-vitro uric acid reducing activities of the two uricase mutants are further verified, and the two uricase mutants can significantly reduce the content of uric acid and are expected to become new uricase drugs for treating hyperuricemia.

The invention belongs to the technical field of fungus inhibitors, and particularly relates to a fungus inhibitor containing origanum vulgare essential oil and a fungus inhibition method. The origanum vulgare essential oil is prepared by mixing the following components: 100-150 parts of origanum vulgare essential oil and 30-50 parts of thyme herb essential oil, the origanum vulgare essential oil is prepared according to the following method: grinding origanum vulgare herb, adding an extraction solvent, performing ultrasonic extraction, then performing steam distillation, collecting distillate, and mixing the distillate with olive oil to obtain the origanum vulgare essential oil, the thyme herb essential oil is prepared according to the following method: crushing thyme herb, adding olive oil, sealing, illuminating, standing for 14-21 days, filtering, and collecting liquid, namely the thyme herb essential oil. The fungus inhibitor containing the oregano essential oil can inhibit aspergillus flavus, penicillium, aspergillus and claviceps purpurea at the same time.

The invention discloses a calcium-based soil conditioner as well as a preparation method and application thereof, and relates to the technical field of soil improvement. The calcium-based soil conditioner comprises straw, calcium salt, nitrogen-phosphorus-potassium fertilizer and acid-resistant bacillus. Compared with the prior art, after the calcium-based soil conditioner provided by the invention is added into the soil, the abundance of Xanthobacraceae (xanthobacteriaceae) and Xanthomonadaceae (xanthomonas family) in the soil is obviously increased, and meanwhile, the abundance of Aspergillus flavus (aspergillus) and the abundance of Rhizopus arrhzizus (rhizopus arrhizus) in the soil are also obviously reduced.

The present invention discloses a method for constructing a mutant uricase prokaryotic expression engineered bacterium, belonging to the field of genetic engineering technology. The present invention performs four active site mutations on a uricase encoding gene derived from Aspergillus flavus to obtain the uricase encoding gene UOX2. A recombinant expression vector for the UOX2 encoding gene is constructed and introduced into Escherichia coli BL21. The resulting uricase-producing prokaryotic expression engineered bacterium is suitable for industrial batch production, and the specific activity of the produced uricase protein reaches 18.3 U / mg, laying a good foundation for large-scale production of uricase.

This invention discloses a strain of *Burkholderia lagoon* and its application in antagonizing *Aspergillus flavus*, belonging to the field of microbial technology. The *Burkholderia lagoon* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38387. This strain can both inhibit the growth of *Aspergillus flavus* and efficiently degrade aflatoxin B1, thus showing good application prospects and value in the field of food production safety technology.

The invention discloses application of yarrowia caribbica NJC36 in prevention and treatment of aspergillus flavus, and belongs to the technical field of biological prevention and treatment. Experiments prove that the yarrowia caribbica NJC36 can inhibit the growth of aspergillus flavus and the synthesis of aflatoxin, and can be used for preventing and treating kiwi fruits from being infected by the aspergillus flavus. The yarrowia caribbica NJC36 disclosed by the invention has a remarkable prevention and treatment effect on aspergillus flavus, can inhibit the synthesis of aflatoxin, and is environment-friendly.

The invention provides a compound preparation for inhibiting aspergillus flavus and application of the compound preparation in prevention and treatment of aspergillus flavus pollution, and the compound preparation comprises a compound microbial agent and a compound extract preparation, the compound microbial agent is prepared by mixing the following microbial agents in parts by weight: 5-10 parts of bacillus sorola, 5-10 parts of bacillus amyloliquefaciens and 3-8 parts of black yeast; the composite extract preparation is prepared by mixing a winged bean extract and a vanilla bean extract according to a weight ratio of (30-55): 1. The plant extract and the microbial agent are compounded to inhibit the aspergillus flavus, the antibacterial effect is good, and a new direction is provided for preventing and treating the aspergillus flavus of grain and oil crops and grain products.