588 results about "Aspergillus flavus" patented technology

The current invention relates to the field of detection and identification of clinically important fungi. More particularely, the present invention relates to species specific probes originating from the Internal Transcribed Spacer (ITS) region of rDNA for the detection of fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cyptococcus neoformans and Pneumocystis carinii in clinical samples, and methods using said probes.

The invention relates to a Bacillus subtilis T-500. The Bacillus subtilis T-500 has been preserved in CGMCC (China General Microbiological Culture Collection), and the preservation number is CGMCC NO:6058. The Bacillus subtilis T-500 can be used as a biological pesticide for preventing and treating multiple plant diseases, and can also be used as a biological fertilizer for promoting the yield increase of crops. The Bacillus subtilis T-500 has higher antibacterial activity on Magnaporthe oryzae, Pellicularia sasakii, Xanthomonas oryzae, Ustilaginoidea virens, Xanthomonas oryzae pv. oryzicola, Fusarium graminearum, Aspergillus flavus, Aspergillus niger and other pathogenic bacteria, and has better prevention and control effects on diseases caused by the pathogenic bacteria. The Bacillus subtilis T-500 can prevent and treat rice leaf diseases when being diluted 200-500 times on the leaves of rice, can prevent and treat bakanae diseases of rice after seed soaking, and can prevent and treat soil-borne diseases of vegetables and the like after root irrigation.

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

The invention discloses a farm fertilizer produced by biologic technology, in particular a decomposing agent for rapidly decomposing straws at low cost, the mixture ratio of which is aerobic bacillus subtilis Cohn 20%, Bacillus licheniformis 10%, aspergillus flavus 20%, chaetomiaceae 20%, absidia corymbifera 20%, and saccharomycetes 10%. The production procedures are: extracting different beneficial decomposed bacteria from organics from different environments, purifying, rejuvenating, breeding and culturing; inoculating the cultured bacterial strains into disinfected solid culture medium; fermenting for 4-5 days under 25-50 DEG C, airing dry so that the residual moisture in the bacterial strains is 25%; mixing, agitating evenly and packing the aired solid bacterial strains into solid decomposing agent. As the invention is made by using a unique optimization and combination of aerobic bacterial group and a high and new technology and production process, the invention is a pure biologic product, is free from smell and harm for making organic fertilizer by effectively decomposing wastes in the countryside, and the fermenting speed is high, the cost is low, and the source of raw material is wide. The invention has improved the soil structure and reduces environmental pollution.

The invention discloses a nano silver antibacterial latex pillow or mattress and a preparation method thereof. Tiny air holes are fully distributed on the surface of the nano silver antibacterial latex pillow or mattress, and air is filled into the tiny air holes; and the silver antibacterial latex pillow or mattress has bacterial resistance on aspergillus niger, aspergillus flavus, discolored aspergillus, penicillium citrinum, paecilomyces varioti, herbarium cladosporium, trichoderma viride and chaetomium globasum. The preparation method comprises the following steps of: uniformly mixing nano sliver with natural latex; then respectively adding sulphur, bi(triethoxy propylsilane)tetrasulfide and 2,6-butylethylene-4-methylphenol; uniformly stirring; adding soap and air to generate foams; then adding gelatin; and compressing for moulding. The nano silver antibacterial latex pillow or mattress of the invention combines air permeability, high elasticity and soft touch feeling of the latex with efficient bacterial resistance and broad spectrum sterilizing performance of nano silver and can effectively overcome the defects of easy bacterial generation, difficult air permeation, and the like of a common pillow on the market.

The invention discloses an Aspergillus flavus strain for not producing toxins of Aspergillus flavus, the Aspergillus flavus strain belongs to the Aspergillus flavus of Aspergillus, the collection name is: Aspergillus flavus strain A051, the collection unit is: China General Microbiological Culture Collection Center, the collection data is: June 24, 2008, and the collection No. is: CGMCC NO.2556. The invention further discloses the use of the Aspergillus flavus strain, and the use is characterized in that: the Aspergillus flavus strain is used for inhibiting Aspergillus flavus pathogenic bacteria groups of the toxins of the Aspergillus flavus in peanut field soil.

The invention discloses a plant essential oil composition for fumigating and killing grain aspergillus flavus. The composition is prepared by utilizing plant or plant essential oil containing cinnamic aldehyde, citral, thymol and salicylaldehyde or by utilizing synthetic identical materials, wherein the parts by weight of the four components in the composition are respectively as follows: 5-20 parts of cinnamic aldehyde, 15-35 parts of citral, 35-60 parts of thymol and 10-35 parts of salicylaldehyde. The composition has the advantages that: the drawing materials are natural, safe and non-toxic; and the volatile essential oil components are easy to diffuse; and the gas of the volatile essential oil is used for fumigating and killing to ensure that the composition needs not be in direct contact with grains and only needs to be put at a corner of a warehouse or a closed container to play a role in efficiently killing the grain aspergillus flavus.

The invention discloses a feed compound enzyme-containing dedicated enzyme for growing pigs and a preparation method of the feed compound enzyme-containing dedicated enzyme, and belongs to the technical field of preparation of enzyme preparations. The dedicated enzyme for the growing pigs is prepared by scientifically compounding aspergillus niger cultures, acidic xylanase, phytase, beta-dextranase, cellulase, amylase, acid proteinase, Chinese herb extracts, a protective agent and an activating agent, wherein the aspergillus niger cells in the aspergillus niger cultures can inhibit the growth of pathogenic escherichia coli, inhibit the growth and breeding of aspergillus flavus, disintegrate the aspergillus flavus, promote the growth of animals, adjust gastrointestinal tract flora to be balanced, and enhance whole immunity during the processes of growth and fermentation, in addition, the fermentation liquid crude enzyme liquid of the aspergillus niger cultures contains different enzyme activity such as protease activity, mannase activity, alpha-galactase activity and pectinase activity, and the dedicated enzyme is specially suitable for being added in the feed for the growing pigs.

The invention discloses a fluorescence quantitative PCR (polymerase chain reaction) universal premier for detecting aspergillus, detection probes and a detection kit, wherein the detection kit comprises universal premiers displayed by base compositions such as SEQ ID. NO: 1 and SEQ ID. NO: 2; and the detection probe is CY5-TAAAGTTGGGTGTCGGCTGG-BHQ aiming at aspergillus fumigatus, the detection probe is FAM-TTGATTTGCGTTCGGCAAGC-BHQ aiming at aspergillus flavus, the detection probe is HEX-ACAAGTTGCAAATAAATGCGTCG-BHQ aiming at aspergillus, and/ or the detection probe is ROX-ATGGTTGGAAAACGTCGGCA-BHQ aiming at aspergillus Niger. The multiple PCR detection method, premier, the probes and the kit thereof provided by the invention aim at four main pathogenic aspergilli and have high specificity and sensitivity; and the detection method is rapid, simple and convenient, and can be used for detecting and identifying the strain of pathogenic bacteria.

The invention discloses a biological special organic fertilizer applied to crops, in particular to a stubble regulating manure that eliminates the block of continuous cropping. The mixture ratio of rotten microbial inoculum is 30 percent of thermophil Bacillus subtilis, 20 percent of Bacillus megatherium, 10 percent of microzyme, 10 percent of Aspergillus flavus, 10 percent of seminude chactomium and 20 percent of Absidia corymbifera; the components of organic fertilizer are of 40 percent of chicken manure, 30 percent of dairy manure and 30 percent of straw powder; the process flow is that: the first fermentation is to graft rotten microbial inoculum in high quality organic fertilizer chicken manure and dairy manure to reach the standard of innocent treatment; the second fermentation is to take the organic fertilizer in the first fermentation as raw material and graft functional bacterial strain to lead the beneficial bacterial count in the organic fertilizer to reach the standard of biological fertilizer. As the invention chooses beneficial strain and adopts unique two-fermentation process, the produced stubble regulating manure can greatly adjust soil ecological balance, inhibit soil-borne disease, decrease the side effect of fertilizer and agricultural chemical, provide full nutrition for the growth of crops and improve the quality of agricultural products.

The invention provides an antagonistic bacterium for controlling radix rehmannia root rot. The antagonistic bacterium is named as pseudomonas aeruginosa LK-12 (the Preservation No.: CGMCC 10966) after authentication. The pseudomonas aeruginosa LK-12 disclosed by the invention is obtained by screening from continuous cropping rhizosphere soil of radix rehmannia root through an agar disk diffusion method. Fusarium oxysporum and aspergillus flavus pathogenic bacteria separated from lesion parts of the radix rehmannia root have a high antagonistic effect; in the meanwhile, fusarium oxysporum, fusarium moniliforme, talaromyces sp. and other pathogenic bacteria separated from lesion parts of radix pseudostellariae also have a remarkable restriction effect and can be used for effectively controlling root rot diseases of medicinal plants comprising the radix rehmannia root to provide a strain for overcoming or relieving replant diseases of the medicinal plants. Therefore, the antagonistic bacterium is a potential biological control strain which is good in biological control effect, free of pollution as well as safe and ecological, and is wide in development and application prospects.

The invention discloses a bacillus amyloliquefacien for degrading aflatoxin B1 in peanut meal, and belongs to the technical field of applied microbiology. According to the invention, a bacillus amyloliquefacien capable of significantly inhibiting the growth of Aspergillus flavus and efficiently degrading aflatoxin B1 can be obtained by screening and the content of aflatoxin B1 in detoxified peanut meal is lower than the national limited standard and achieves the safe feeding level after the bacillus amyloliquefacien is applied to moldy peanut meal. Furthermore, the detoxification mechanism of the strain is the degradation effect of extracellular metabolites, and generation of the active substance is of a non-induced type, which is an inherent attribute of the strain. The features of the strain provide the possibility for biocontrol of Aspergillus flavus and aflatoxin B1 in industries such as feed and foods and the strain has very high application values.

The present invention relates to preparation method of aflatoxin immuno-affinity column, including steps of: preparation of hydrazine antibody: employing sodium periodate oxidating unconjugated active area Fc hydroxoy of antibody to aldehyde group, and reacting with adipic dihydrazide to gain hydrazine antibody; preparation of aldehyde solid substrate: oxidating solid substrate containing continued diol structure by sodium periodate to gain aldehyde solid substrate; coupling of antibody and solid substrate: mixing hydrazine antibody and aldehyde solid substrate, adding sodium borohydride to seal unreacted aldehyde group on solid substrate after oscillating reaction to gain coupling antibody solid substrate; column packed. The present invention couples the unconjugated active area Fc end of antibody to solid substrate to remain activity of antibody better; hydrazide derived on antibody instead of solid substrate to avoid nonspecific adsorption of hydrazide group; the combined affinity column not introducing new groups ( such as carboxyl, amido and so on) except coupling antibody to avoid nonspecific adsorption from them.

The invention relates to a block board with antibacterial and anti-mildew functions, and a preparation method thereof, which belongs to the building material field. The invention aims at providing a block board which can kill and control the exterior bacteria and prevent the exterior mildew from growing, and the preparation method thereof, inorganic antibacterial material and organic anti-mildew material are added in the adhesive stuck on the surface layer of the block board, the adhesive of the antibacterial and anti-mildew materials is coated on the thick veneer of the block board, then the wood veneer on the outmost surface layer is painted, cold-pressing treatment and hot-pressing treatment are performed in sequence, and the antibacterial and anti-mildew materials seep into the surface layer and play a role. The block board with the antibacterial and anti-mildew functions can realize the purpose that the inhibiting rate of the representative bacteria is larger than or equal to 90 percent within 24 hours, such as staphylococcus aureus, escherichia coli, and the like; the mould growth grade of six fungi is zero grade or one grade within 28 days, such as aspergillus niger, aspergillus flavus, and the like, and the block board has good and long-lasting antibacterial and anti-mildew functions.

The invention discloses a biosensor electrode for detecting aflatoxin B1 and a preparation method thereof. The biosensor electrode comprises a substrate layer and a reaction layer provided on the substrate layer. The reaction layer includes an electronic mediator, a sol-gel film and aflatoxin oxidase, wherein the electronic mediator is fixed on the substrate layer, and the sol-gel film embeds the aflatoxin oxidase on the substrate modified by the electronic mediator to form a film, thus obtaining aflatoxin oxidase modified electrode. The inventive biosensor electrode has the advantages of fast response, high signal sensitivity up to 1.5 ppm to 0.125 ppb, high specificity, convenient usage, quantitative detection, long service life and good stability.

The invention relates to a method for realizing accurate and quick detection of aflatoxin. The invention is characterized in that the method is as follows: step (1), enzyme-labelled antigen, an aflatoxin AFB1 standard solution, sample diluent and enzyme-labelled antigen diluent are made, and a substrate solution a, a substrate solution b, a stop solution (i.e. a 2mol/L sulphuric acid solution) and extract are made by a cleaning solution; step (2), to-be-measured solution pretreatment, reagent preparation and reaction plate test tube numbering are carried out, and a prepared solution, a to-be-measured sample solution and the stop solution are added in turn; meanwhile, a reaction plate is arranged, and an aflatoxin tester is used to measure the absorbance A value of each hole; then, according to the measured absorbance A value, an equation A=MC+N for regression calculation is automatically established inside the aflatoxin tester to calculate the content of AFB1 in sample. The method has the advantages of simplicity and convenience, quickness, high accuracy and high sensitivity.

The invention belongs to the field of peanut oil extraction, and discloses a protogenic virgin peanut oil and a production process thereof. The production process comprises the following steps: (1) separating and screening; (2) peeling off the red peanut coat and stone; (3) performing magnetic separation and color separation; (4) performing chemical treatment; (5) sterilizing by using ozone; (6) baking; (7) squeezing the oil; (8) removing phospholipid. The invention further discloses the peanut oil prepared by using the production process. By adoption of the production process disclosed by the invention, the unique fragrance of peanut is retained, and harmful substances such as aspergillus flavus are thoroughly removed.

The invention provides a distiller's yeast and a production method thereof. In the production method of the distiller's yeast, the distiller's yeast comprises the following raw materials in parts by weight: 3 parts of barley, 4 parts of wheat, 1.5 parts of soybean and 1.5 parts of peas; the adopted seed koji comprises the following raw materials by weight percent: 0.1% of aspergillus oryzae box seed koji, 0.1% of aspergillus flavus box seed koji, and 0.1% of aspergillus niger; the production method comprises the following steps: adding raw materials including barley, wheat, soybean and peas into aspergillus oryzae box seed koji, aspergillus flavus box seed koji, and aspergillus niger, evenly mixing, introducing a ventilating koji pond, adjusting the temperature to be 30-35DEG C, and standing and culturing for 6-8 hours; performing first koji turning after the koji is cultured for 12 hours in the pond, and keeping the normal temperature of 30-35DEG C; continuously culturing for 4-6 hours, and performing second koji turning; culturing for up to 24-28 hours; and drying to obtain the distiller's yeast. The distiller's yeast is prepared by adopting the production method. The distiller's yeast has stable quality, the defect existing during the process of producing the yeast from raw materials in the prior art can be avoided, and the production period can be shortened.

The invention relates to a lactobacillus pentosus strain and a fermentation product of the same. The preservation number of the lactobacillus pentosus LPEM818 in the invention is CGMCC No. 4583 in the China General Microbiological Culture Collection Center. The fermentation product of the lactobacillus pentosus in the invention can inhibit common pathogenic escherichia coli, pseudomonas aeruginosa and staphylococcus aureus in food, and particularly has a strong inhibiting effect on listeria monocytogenes which can cause serious food poisoning. In addition, the metabolite of the strain also can inhibit the growth of aspergillus flavus and cotton and tomato blight pathogens. Consequently, the lactobacillus pentosus strain and the fermentation product thereof play an active role in the fields of food preservation and plant protection.

A formulation containing conidia of non-toxigenic strains of fungi is a useful biocontrol agent for preventing toxin contamination in agricultural commodities, especially those for human and animal consumption such as peanuts, corn, cotton and tree nuts. The formulation of the invention is a water dispersible granule formulation suitable for spraying and includes non-toxigenic and/or non-aflatoxigenic Aspergillus flavus strains capable of inhibiting growth of fungi which produce aflatoxin and further capable of suppressing production of aflatoxin by the toxigenic fungi. A method of preparing the formulation is shown.

The invention provides a process for preparing the lactone of the intermediate of dextral biotin. The invention employs racemic acid 2 (1, 3-dibenzyl-5(hydroxymethyl)-2-oxo-4-Imidazolidinecarboxylic aid) which is achieved by the hydrolyzing the lactone 1 of the intermediate of racemization biotin 1 (tetrahydro-1, 3-bis (phenylylmethyl)-1 H-Furo (3, 4-d) imidazole-2, 4-dione) is utilized as reaction substrate, and the lactone of the intermediate of dextral biotin 9 is selectively synthesized in the function of lactone synthase. Further, the lactone synthase is one of the following materials, including (1) pig liver esterase, (2) pancreatin, (3) aspergillus oryzae lipase, (4) aspergillus niger lipase, (5) aspergillus flavus lipase, (6) blue mold lipase, (7) rhizopus chinensis lipase, and (8) yeast lipase. The beneficial effects of the process for preparing the lactone of the intermediate of dextral biotin include that resolution specificity is high, the resolution efficiency is high, the manufacture cost is greatly reduced, the operation is simple, the environmental pollution is low, and the invention is adaptable for industrial production.

The invention relates to a disinfectant for culturing silkworms, which uses bromochlorohydantoin as a main agent and an alkaline sodium salt as an auxiliary agent, wherein when the disinfectant is prepared into solution by using water as a solvent, the mass volume density of the bromochlorohydantoin is 120 to 960 mg/L, and the mass percentage concentration of the auxiliary agent is 0.25 to 0.75 percent. When the disinfectant is prepared into powder, the mass percentage of the bromochlorohydantoin is 5 to 20 percent, and the mass percentage of the corresponding auxiliary agent is 80 to 95 percent. The alkaline sodium salt is any one of trisodium phosphate, chlorinated trisodium phosphate or sodium bicarbonate. More preferably, the mass volume concentration of the bromochlorohydantoin is 240 to 480 mg/L, and the mass percentage concentration of the alkaline sodium salt is 0.45 to 0.5 percent. The disinfectant develops novel application of the existing medicament bromochlorohydantoin; the disinfectant prepared from the bromochlorohydantoin and an alkaline compound of the trisodium phosphate, the chlorinated trisodium phosphate or the sodium bicarbonate and the like is safe and reliable, has a good effect of killing main pathogens of NPV, CPV, N. b, beauveria bassiana, aspergillus flavus, bacillus thuringiensis and toxins, and is an effective broad-spectrum disinfectant for culturing the silkworms.

The present invention discloses a special biological organic fertilizer used for the economical crops, in particular to an amino acid fragrant-increasing fertilizer used for tobacco, tea and vegetable. The components and the weight ratio for the fragrant-increasing bacterium agent microorganism are that: 20 percent of Bacillus subtilis, 20 percent of sodium bean Bacillus, 20 percent of lactobacillus, 20 percent of microzyme and 20 percent of Aspergillus flavus; the components for the fermenting materials are that: 90 percent of vegetable grain, 9 percent of fish powder and 1 percent of vinegar residue. The technics flow is that: the microbe bacterium seed is inoculated into a high-density ferment pot system with single seed and then is respectively to be made with expanding propagation; then the solid or liquid fragrant-increasing bacterium agent can be prepared; and then the high protein materials are added into the organic fertilizer, so the amino acid fragrant-increasing fertilizer can be finished. Because of adopting the technics method for a special high-density fermenting system to foster the material, the produced amino acid fragrant-increasing fertilizer is abundant of amino acid and organic substance. So the present invention can enhance the fragrant quality for tobacco, tea and vegetable, adjust the soil zoology balance, restrict the disease and enhance the quality of the economical crops.