95 results about "Aspergillus flavus" patented technology

The invention discloses a method for detecting aspergillus flavus and aflatoxin B1 of crops based on an infrared spectrum. The method comprises the steps of crop sample material collection, rice multi-strain mould infection, spectrum collection, crop sample aflatoxin B1 determination, spectrum pretreatment, correction model construction and model verification. The invention aims at detecting the content of aspergillus flavus infection and toxin B1 thereof in crops by combining a near-infrared spectrometer and a stoichiometric method. The method has the advantages of being simple and easy to implement, high in analysis speed, high in detection precision, low in analysis cost, free of any pollution to the environment and the like, samples do not need any pretreatment, and the method has important practical value for guaranteeing the quality safety of agricultural products and reducing the carcinogenic risk of people.

The invention discloses a Trichoderma reesei strain and an application of the Trichoderma reesei strain in degradation of mycotoxin and inhibition of toxin-producing bacteria. The Trichoderma reesei strain is a Trichoderma reesei strain GG-T40, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20231843. The strain can synchronously and efficiently degrade eight kinds of mycotoxins such as AFB1, ZEN, T-2, ST, OTA, AOH, AME and TeA, and after the strain and the mycotoxins are co-cultured for 7 days, the removal rates of the eight kinds of toxins are all larger than or equal to 60%. The strain has strong bacteriostasis and toxin production inhibition effects, the toxin production inhibition rates of the strain to aspergillus flavus and fusarium graminearum are 62.5% and 74.4% respectively, and the strain can be applied to degradation of mycotoxin in agricultural products and food and inhibition of mildew and toxin production of the agricultural products and food.

The invention relates to a microbial agent based on a phosphate tailing storage yard and application of the microbial agent, the microbial agent based on the phosphate tailing storage yard comprises a talaromyces new species Talaromyces coriarcola KY-QH135, which is preserved in China Center for Type Culture Collection in Wuhan University with the number of CCTCC M 20252753, and the microbial agent based on the phosphate tailing storage yard is preserved in China Center for Type Culture Collection in Wuhan University with the number of CCTCC M 20252753. The invention relates to the field of biotechnology, and in particular relates to a biotechnology which is selected from one or more of Aspergillus tubingensis, Aspergillus flavus, Aspergillus oryzae, Aspergillus bombycis and Aspergillus niger, as well as one or more of Aspergillus tubingensis, Aspergillus flavus, Aspergillus oryzae, Aspergillus bombycis and Aspergillus niger.

The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus capable of inhibiting growth of monascus and application of the plant lactobacillus. The preservation number of the plant lactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.34851. According to the present invention, the plant lactobacillus CCNH295 has strong bacterial inhibition ability on common soybean meal contaminating molds, such as aspergillus flavus, aspergillus ochraceus, fusarium moniliforme and penicillium; the plant lactobacillus CCNH295 has excellent bacteriostatic ability to monascus, and when the plant lactobacillus CCNH295 is used for fermentation production of soybean meal, growth of monascus can be remarkably inhibited, so that mildewing of the fermented soybean meal is effectively inhibited, and the prepared fermented soybean meal does not mildew after being placed at 37 DEG C for 6 months; besides, the fermented soybean meal produced by solid state fermentation of the plant lactobacillus CCNH295 is used as a feed raw material, so that the stability of the feed can be improved, and the palatability of the feed can be improved.

The invention discloses a urate oxidase mutant (rAfUOX) with trypsin and chymotrypsin tolerance and application of the urate oxidase mutant (rAfUOX). The invention provides a urate oxidase mutant with trypsin and chymotrypsin tolerance by modifying a key amino acid residue in a wild type aspergillus flavus urate oxidase molecule through a protein engineering technology. The activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after trypsin digestion is increased by 244% compared with the activity half-life period of the wild type rAfUOX, and the activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after chymotrypsin digestion is increased by 191% compared with the activity half-life period of the wild type rAfUOX. Other enzymatic properties are basically consistent with those of the wild type rAfUOX.

The invention discloses a strain of bradyrhizobium guangzhouense APHBXY-23-1 with functions of efficiently promoting growth and strongly inhibiting aspergillus flavus and application of the strain of bradyrhizobium guangzhouense APHBXY-23-1. The preservation number of the strain of bradyrhizobium guangzhouense APHBXY-23-1 is CCTCC (China Center for Type Culture Collection) M 20253048. The invention not only retains the core functions of the strain as rhizobium for efficient symbiotic nitrogen fixation and crop growth promotion, but also reveals that the strain can significantly inhibit the growth of aspergillus flavus by releasing a volatile substance dimethylbutyric acid for the first time. The discovery breaks through the technical limitation that the traditional rhizobium only has a growth promoting function and lacks a biocontrol effect, the two agricultural microorganism core functions of nitrogen fixation and growth promotion and biocontrol bacteriostasis are endogenously integrated into the same microorganism carrier for the first time, and a novel rhizobium resource with double functions is successfully constructed; a new species of growth-promoting rhizobium with a biocontrol function is created, and the growth-promoting rhizobium has important innovative value in theory and application levels.

The application provides an Aspergillus flavus and an enzyme production method and application thereof, and belongs to the technical field of microorganisms.The Aspergillus flavus (Aspergillus flavus) HNZY01 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.42147.The Aspergillus flavus HNZY01 has the ability of high-yield cellulase, can efficiently and synergistically decompose key structural components such as cellulose, hemicellulose and lignin in tobacco stems, and can also increase the content of reducing sugar in the tobacco stems, so that the Aspergillus flavus HNZY01 can be applied in the degradation of the tobacco stems and the smoking safety of cigarettes can be improved.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

The invention discloses sinorhizium xinjiangense APXJHT24-1 which has the functions of promoting growth and inhibiting aspergillus flavus, and the preservation number of the sinorhizium xinjiangense APXJHT24-1 is CCTCC (China Center for Type Culture Collection) M 2026229. According to the invention, the integration of two major functions of'generation of a characteristic antibacterial volatile matter of dimethyl disulfide 'and'efficient symbiotic nitrogen fixation' on a single strain is realized in Sinkiang Sinkiang rhizobium for the first time. The dimethyl disulfide released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in the aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.

The invention discloses a uricase mutant. The uricase sequence from aspergillus flavus and the uricase sequence from candida are taken as templates, multiple rounds of random mutation are carried out, sequencing verifies that the key amino acid site of the catalytic center is mutated, and the catalytic activity is enhanced. In-vivo and in-vitro uric acid reducing activities of the two uricase mutants are further verified, and the two uricase mutants can significantly reduce the content of uric acid and are expected to become new uricase drugs for treating hyperuricemia.

The invention discloses a calcium-based soil conditioner as well as a preparation method and application thereof, and relates to the technical field of soil improvement. The calcium-based soil conditioner comprises straw, calcium salt, nitrogen-phosphorus-potassium fertilizer and acid-resistant bacillus. Compared with the prior art, after the calcium-based soil conditioner provided by the invention is added into the soil, the abundance of Xanthobacraceae (xanthobacteriaceae) and Xanthomonadaceae (xanthomonas family) in the soil is obviously increased, and meanwhile, the abundance of Aspergillus flavus (aspergillus) and the abundance of Rhizopus arrhzizus (rhizopus arrhizus) in the soil are also obviously reduced.

This invention discloses a strain of *Burkholderia lagoon* and its application in antagonizing *Aspergillus flavus*, belonging to the field of microbial technology. The *Burkholderia lagoon* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38387. This strain can both inhibit the growth of *Aspergillus flavus* and efficiently degrade aflatoxin B1, thus showing good application prospects and value in the field of food production safety technology.

The invention discloses application of yarrowia caribbica NJC36 in prevention and treatment of aspergillus flavus, and belongs to the technical field of biological prevention and treatment. Experiments prove that the yarrowia caribbica NJC36 can inhibit the growth of aspergillus flavus and the synthesis of aflatoxin, and can be used for preventing and treating kiwi fruits from being infected by the aspergillus flavus. The yarrowia caribbica NJC36 disclosed by the invention has a remarkable prevention and treatment effect on aspergillus flavus, can inhibit the synthesis of aflatoxin, and is environment-friendly.

The invention provides a compound preparation for inhibiting aspergillus flavus and application of the compound preparation in prevention and treatment of aspergillus flavus pollution, and the compound preparation comprises a compound microbial agent and a compound extract preparation, the compound microbial agent is prepared by mixing the following microbial agents in parts by weight: 5-10 parts of bacillus sorola, 5-10 parts of bacillus amyloliquefaciens and 3-8 parts of black yeast; the composite extract preparation is prepared by mixing a winged bean extract and a vanilla bean extract according to a weight ratio of (30-55): 1. The plant extract and the microbial agent are compounded to inhibit the aspergillus flavus, the antibacterial effect is good, and a new direction is provided for preventing and treating the aspergillus flavus of grain and oil crops and grain products.

The invention belongs to the technical field of biological mildew preventive, and discloses bacillus licheniformis fermentation liquor and application thereof in mildew prevention and quality improvement of silage. The fermentation liquor is prepared by taking bacillus licheniformis CMCC 63516 as a strain under optimized conditions (with an LB culture medium, the inoculum size of 6%, the liquid loading amount of 30% and culture for 18 hours), and the viable count can reach 1.48 * 10 < 9 > CFU / mL. The strain has a remarkable antagonistic effect on toxin-producing aspergillus flavus, can efficiently degrade aflatoxin B1 (the degradation rate gt in 36 hours is 96%), and has good environmental stability. The additive amount of 1.2-4.0% is applied to corn silage, the growth of mould and saccharomycetes can be remarkably inhibited, the content of AFB1 is greatly reduced, and the mould-proof effect is superior to that of a chemical mould-proof agent sodium diacetate. In addition, the fermentation liquor can also effectively improve the quality of silage, increase the content of crude protein and lactic acid, and reduce the pH and the content of ammoniacal nitrogen and detergent fibers. The invention provides a safe, environment-friendly and efficient biological mildew-proof method which has a wide application prospect in the feed industry.

The application discloses an Aspergillus luchuensis YZ-1 and application thereof, and belongs to the field of microorganism safety.The application separates a new strain from a black tea in Guilin, Guangxi, identifies the strain by ITS, beta tubulin and calmodulin, and the identification result is Aspergillus luchuensis, and the preservation number is CCTCC NO: M 20221462.It is verified by experiments that the strain can inhibit various bacteria and fungi, the water extract and fermentation liquor of the strain can significantly inhibit the growth of Aspergillus flavus, the fermentation liquor has a strong inhibiting effect on the growth of Aspergillus flavus, and the strain has a strong adsorbing effect on aflatoxin B1.Therefore, the Aspergillus luchuensis disclosed by the application has important application value in preventing and controlling mold pollution in grain crops and food.

This invention discloses a Litsea cubeba essential oil preparation, which is prepared by mixing a 1% (w / w) chitosan quaternary ammonium salt solution with a Litsea cubeba essential oil ethanol mixture at a volume ratio of 4:1; the volume ratio of Litsea cubeba essential oil to anhydrous ethanol in the Litsea cubeba essential oil ethanol mixture is 2:3. The Litsea cubeba essential oil preparation prepared by this invention exhibits good dispersibility and stability, achieving 100% short-term and long-term antibacterial effect against Aspergillus flavus. This is significantly superior to using Litsea cubeba essential oil or chitosan quaternary ammonium salt alone, as well as other chitosan quaternary ammonium salt-Litsea cubeba essential oil preparations, and even more superior to commonly available antifungal agents. Its preparation method is simple, non-toxic, harmless, and does not pollute the environment. It has low production costs and can be used to prevent or inhibit the growth of Aspergillus flavus, reducing its damage to wooden household products.

The present application provides a mildew inhibitor and its application in tobacco recombination raw materials. The mildew inhibitor mainly comprises clove compound essential oil composed of clove bud essential oil, star anise essential oil and cassia essential oil, and the mass ratio of the clove bud essential oil, the star anise essential oil and the cassia essential oil is 10:(0-4):(0-6). Through tests, the clove compound essential oil can inhibit the growth of various molds such as Aspergillus flavus, Aspergillus niger and Aspergillus montgenieri. The mildew inhibitor has a broad-spectrum inhibitory effect on common Aspergillus of tobacco leaves, can be used as a tobacco mildew inhibitor, can be used for preventing and treating the mildew of tobacco recombination raw materials, and can be in harmony with the tobacco aroma of heated cigarette smoke, so that the mildew inhibition and aroma enhancement effects on the tobacco recombination raw materials are achieved. In addition, as a tobacco mildew prevention additive, the application method of the mildew inhibitor is simple, diverse and adjustable, the integration degree with the core material preparation process is high, and the mold growth inhibition effect is obvious.

The invention discloses bacillus subtilis, a microbial agent and application of the bacillus subtilis, the bacillus subtilis is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC 35166. The invention also provides a preparation method and application of the strain and a storage method of plant seeds. The strain disclosed by the invention has aspergillus flavus resisting activity, and can be used for dressing rice seeds, reducing seed mildewing and promoting seed germination.

This invention relates to the X178 strain, which inhibits fungal diseases in grains and removes mycotoxins, and its applications. The X178 strain belongs to the Nocardiaceae family (…). Nocardiopsis This fungus (sp.) is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 34027. Specifically, it exhibits inhibitory effects against grain pathogenic fungi Aspergillus flavus, Aspergillus niger, and Fusarium graminearum, and can remove two mycotoxins: aflatoxin B1 (AFB1) and / or zearalenone.

The invention discloses a bacillus velezensis ATC-AL and application thereof. The preservation number of the strain is CCTCC (China Center For Type Culture Collection): M20251280. A genome of the bacillus velezensis ATC-AL has a synthetic gene cluster for synthesizing various antifungal active substances, the active substances of the bacillus velezensis ATC-AL can destroy cell surface structures of pathogenic bacteria, induce cell apoptosis and inhibit spore development of pathogenic fungi, and the bacillus velezensis ATC-AL has a broad-spectrum inhibition effect on various pathogenic fungi such as sweet potato coracocystis canaliculata, fusarium graminearum and aspergillus flavus; the generation inhibition rate of vomitoxin reaches 95%, and the generation inhibition rate of aflatoxin B1 can reach 100%; fungus infection experiments on sweet potatoes, corn and wheat prove that active substances in a strain cell fermentation broth can completely inhibit the decay effect of sweet potato coracocystis longirostris, fusarium graminearum and aspergillus flavus on the food crops; according to the invention, a microbial control resource library for biological control of pathogenic fungi and toxin pollution thereof is enriched.

The invention discloses sinorhizobium meliloti ASZJHZ25-4 with functions of efficiently promoting growth and inhibiting aspergillus flavus and application of the sinorhizobium meliloti ASZJHZ25-4, and the preservation number of the sinorhizobium meliloti ASZJHZ25-4 is CCTCC M 2026230. According to the invention, the integration of two functions of generating a characteristic antibacterial volatile matter of 2-methylbutyric acid and efficiently symbiotic nitrogen fixation on a single strain is realized in the sinorhizobium meliloti for the first time. The 2-methylbutyric acid volatile matter released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis can be synchronously achieved at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer is formed in a soil microenvironment in a legume development critical period.

According to the method for extracting and purifying the bacteriostatic active substances in the bacillus velezensis B2 fermentation liquor and the application, the micromolecular substances (SLCs) with remarkable bacteriostatic activity are successfully separated by optimizing an extracting solvent, determining n-butyl alcohol as an optimal extracting agent and combining technologies such as silica gel column chromatography, gel column chromatography and preparative liquid chromatography; the substance has a remarkable inhibition effect on spore germination and mycelial growth of aspergillus flavus, and the minimum inhibition concentration is 0.5 mg / mL. Through observation of a scanning electron microscope, the morphology of the aspergillus flavus spores and hyphae treated by the BSIF is obviously changed, and the morphology shows that the spores are sunken, and the hyphae are shrunk and broken. The invention provides a new active substance source for biological prevention and control of aspergillus flavus, and has a wide application prospect.

Aspergillus flavus is an opportunistic, saprophytic fungus that infects maize and other fatty acid-rich food and feed crops and produces toxic and carcinogenic secondary metabolites known as aflatoxins. In vitro studies showed a five-fold increase in antifungal activity of AGM182 (vs. tachyplesin1) against A. flavus. Transgenic maize plants expressing AGM182 under maize Ubiquitin-1 promoter were produced through Agrobacterium-mediated transformation. PCR products confirmed integration of the AGM182 gene, while RT-PCR of maize RNA confirmed the presence of AGM182 transcripts. Maize kernel screening assay using a highly aflatoxigenic A. flavus strain (AF70) showed up to 72% reduction in fungal growth in the transgenic AGM182 seeds compared to isogenic negative control seeds.

The invention discloses a method for efficiently screening rhizobium with functions of promoting growth and inhibiting aspergillus flavus and application of the method, the field ecological pre-enrichment effect of a microbial agent with an ARC function is introduced into a rhizobium screening process for the first time, and the abundance of a target flora is directionally increased from the source through the toxin control and bacterium enrichment effect of the rhizobium. The method comprises the following steps: synchronously collecting rhizosphere soil, root system and root nodule samples, and comprehensively separating rhizobium; a high-throughput primary screening system with parallel growth-promoting and antibacterial functions is constructed, and the nitrogen-fixing growth-promoting capability and the antibacterial effect of volatile substances of candidate strains are synchronously evaluated in an early stage through a pot experiment and a double-vessel buckling method. And further performing functional quantification, multi-gene phylogenetic identification and omics mechanism analysis on the positive strain. The method overcomes the defects that a traditional method is single in function and low in efficiency, and an effective way for rapidly, directionally and systematically screening multifunctional rhizobium resources from an optimized rhizosphere ecosystem is provided.

This invention discloses a method and kit for rapid detection of deeply infected Aspergillus. The method involves mixing the sample to be tested with a nucleic acid cleavage extract and heating at 60–65°C for 8–20 min, followed by heating at 93–98°C for 5–10 min to obtain a DNA extract. This DNA extract is then added to a PCR premix containing a composition of deeply infected Aspergillus to prepare a PCR reaction solution. The PCR reaction solution is subjected to the following PCR amplification reactions: denaturation is performed at 90–100°C for 0–5 s, followed by annealing and extension at 50–70°C for 0–5 s, repeated 35–50 times. This method not only provides a specific primer and probe composition capable of distinguishing between Aspergillus flavus, Aspergillus niger, and Aspergillus fumigatus, but also enables rapid detection of the sample from nucleic acid extraction to nucleic acid amplification, saving detection time.

The invention discloses a sinorhizobium fredii strain capable of promoting growth and inhibiting aspergillus flavus and application of the sinorhizobium fredii strain. The sinorhizobium fredii strain is classified and named as sinorhizobium fredii, and the preservation number of the sinorhizobium fredii strain is CCTCC (China Center for Type Culture Collection) M 20253054. According to the invention, the dual functions of generating a specific bacteriostatic volatile matter 2-nonanol and efficiently symbiotic nitrogen fixation are integrated in the sinorhizobium freudenreanum for the first time. 2-nonanol released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.

The invention is applicable to the technical field of microorganisms, and provides aspergillus flavus and application thereof in prevention and treatment of fall webworm, the aspergillus flavus is aspergillus flavus YZNUH-01, and is preserved in China General Microbiological Culture Collection Center on February 11, 2025, the address is No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 41809. The specific high pathogenicity of the aspergillus flavus YZNUH-01 to the fall webworm is explored and verified for the first time, brand new fungus resources with different action mechanisms are provided for biological control of the fall webworm, and the aspergillus flavus YZNUH-01 is good in control effect and environmentally friendly.

This invention discloses a method for preparing camphor root volatile oil, which is used for anti-oxidation, anti-aspergillus flavus, and inhibition of aflatoxin synthesis. The camphor root volatile oil of this invention is extracted by steam distillation, with a maximum volatile oil content of 3.34%. Gas chromatography-mass spectrometry analysis of the chemical composition of the camphor root volatile oil confirmed that the main components include camphor, 1,8-cineole, linalool, α-pinene, and sapinene. When applied to inhibit the growth and toxin production of Aspergillus flavus, a volatile oil concentration of 3 μL / mL completely inhibits aflatoxin production. This volatile oil also possesses antioxidant activity, with a half-maximal inhibitory concentration (IC50) for scavenging ABTS free radicals. 50 Below DPPH radicals, its lowest IC50 value is... 50 The values ​​were 0.6115±0.4240 mg / mL and 1.7658±0.2488 mg / mL, respectively. This volatile oil can also inhibit and control Aspergillus flavus contamination in Job's tears, and has application value in the food and health industries.

The invention relates to the technical field of forage grass ensiling fresh-keeping, in particular to a method for inhibiting growth of mould hyphae in ensiling forage grass, which comprises the following steps: 1) preparing targeted straw vinegar: adopting a dry distillation method, cooling and condensing, standing and layering, filtering and adsorbing and purifying by activated carbon, and accurately controlling the content of acetic acid to be 30-40% and the content of phenolic compounds to be 15-20%; 2) adding a composite probiotic protection system: spraying targeting straw vinegar liquid accounting for 0.5-5% of the weight of the raw materials into the silage raw materials, and synchronously adding 0.1-0.3% of a composite protective agent; 3) regulating and controlling the ensiling environment in stages: the temperature is 25-30 DEG C and the humidity is 60-70% in the first 10 days, the temperature is 20-25 DEG C and the humidity is 60-70% in the later 20-35 days, and the total ensiling time is 30-45 days; and 4) dual detection verification: detecting mycotoxin by HPLC-MS / MS, and detecting the number of beneficial bacteria by a plate counting method.Experiments show that the method has an aspergillus flavus and aspergillus fumigatus inhibition rate of more than 65%, a lactobacillus survival rate of more than 90%, no residue of straw vinegar, and low cost, and is suitable for silage of various forage grass such as corn straw, alfalfa and the like.