130 results about "Aspergillus flavus" patented technology

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The invention discloses a method for detecting aspergillus flavus and aflatoxin B1 of crops based on an infrared spectrum. The method comprises the steps of crop sample material collection, rice multi-strain mould infection, spectrum collection, crop sample aflatoxin B1 determination, spectrum pretreatment, correction model construction and model verification. The invention aims at detecting the content of aspergillus flavus infection and toxin B1 thereof in crops by combining a near-infrared spectrometer and a stoichiometric method. The method has the advantages of being simple and easy to implement, high in analysis speed, high in detection precision, low in analysis cost, free of any pollution to the environment and the like, samples do not need any pretreatment, and the method has important practical value for guaranteeing the quality safety of agricultural products and reducing the carcinogenic risk of people.

The invention discloses streptomyces allogeri and an application thereof in biological prevention and control of toxin-producing fungi. In particular to streptomyces allorgeus ZxBac193 CGMCC (China General Microbiological Culture Collection Center) No.32341 and an application thereof. The streptomyces allorgeus ZxBac193 shows a strong antagonistic effect on toxin-producing fungi such as aspergillus flavus, aspergillus ochraceus, fusarium graminearum, fusarium oxysporum, penicillium citrinum and penicillium islanigerum; the compound can inhibit the growth of aflatoxin producing fungi, ochratoxin producing fungi, deoxynivalenol producing fungi, zearalenone toxin producing fungi, fumonisins producing fungi, citrinin producing fungi and island penicillin producing fungi, and can be prepared into various biological control products aiming at mycotoxins, such as biopesticides, antifungal agents and the like. The compound is used for inhibiting toxic fungi in the environment and preventing or relieving mycotoxin pollution, and has good development and application prospects in the fields of prevention and treatment of fungal pollution of food crops and the like.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention discloses a mycotoxin degrading bacterium (Lactobacillus plantarum) R4-30 and application thereof, the strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M2018437, and the strain is obtained by screening in Guangxi sugarcane tail leaf silage. The strain can inhibit the growth of aspergillus flavus and fusarium graminearum, so that the production of mycotoxin is reduced, and the mycotoxin can be degraded. The aflatoxin B1 degradation rate of the strain is as high as 91.21% when the bacterial concentration is 12 * 10 < 8 > cfu / ml, the pH value is 2 and the culture time is 16 days; when the bacterium concentration is 3 * 10 < 8 > cfu / ml, the pH value is 6 and the culture time is 12 days, the zearalenone degradation rate is as high as 94.56%; when the bacterial concentration is 12 * 10 < 8 > cfu / ml, the pH value is 2 and the culture time is 16 days, the degradation efficiency of AFB1 and ZEN in the mango peel fermented feed is remarkably improved, the degradation rates of AFB1 and ZEN under the conditions reach 91.2% and 58.16% respectively, the nutritional quality is high, the fermentation quality is good, chemical additives are not needed, and the method is suitable for green detoxification treatment of agricultural products and feed.

The invention discloses a Trichoderma reesei strain and an application of the Trichoderma reesei strain in degradation of mycotoxin and inhibition of toxin-producing bacteria. The Trichoderma reesei strain is a Trichoderma reesei strain GG-T40, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20231843. The strain can synchronously and efficiently degrade eight kinds of mycotoxins such as AFB1, ZEN, T-2, ST, OTA, AOH, AME and TeA, and after the strain and the mycotoxins are co-cultured for 7 days, the removal rates of the eight kinds of toxins are all larger than or equal to 60%. The strain has strong bacteriostasis and toxin production inhibition effects, the toxin production inhibition rates of the strain to aspergillus flavus and fusarium graminearum are 62.5% and 74.4% respectively, and the strain can be applied to degradation of mycotoxin in agricultural products and food and inhibition of mildew and toxin production of the agricultural products and food.

The invention relates to the field of microorganisms, in particular to bacillus subtilis, a fungicide and application of the fungicide. The invention discloses a bacillus subtilis strain, which is classified and named as bacillus subtilis, has a strain number of GXGD-YZ-2483, is preserved in Guangdong Microbial Culture Collection Center on March 13, 2025, and has a preservation number of GDMCC No: 66014. The bacillus subtilis can tolerate the high temperature of 70-90 DEG C, has excellent self-heat production performance, and can produce lignin degrading enzymes (laccase, manganese peroxidase and / or lignin peroxidase) and inhibit the growth of pathogenic bacteria (escherichia coli, salmonella, clostridium perfringens and / or aspergillus flavus). When the bacillus subtilis or the microbial inoculum prepared from the bacillus subtilis is used for biologically composting organic wastes, a lignin barrier can be efficiently cracked by secreting a lignin degrading enzyme system, lignin degradation, nitrogen retention and pathogenic bacteria inactivation can be synchronously realized by virtue of high temperature resistance, self-heat production characteristics and enzyme activity stability, the composting period is shortened, and the yield of the organic wastes is improved. The humification quality is obviously improved.

The invention relates to a microbial agent based on a phosphate tailing storage yard and application of the microbial agent, the microbial agent based on the phosphate tailing storage yard comprises a talaromyces new species Talaromyces coriarcola KY-QH135, which is preserved in China Center for Type Culture Collection in Wuhan University with the number of CCTCC M 20252753, and the microbial agent based on the phosphate tailing storage yard is preserved in China Center for Type Culture Collection in Wuhan University with the number of CCTCC M 20252753. The invention relates to the field of biotechnology, and in particular relates to a biotechnology which is selected from one or more of Aspergillus tubingensis, Aspergillus flavus, Aspergillus oryzae, Aspergillus bombycis and Aspergillus niger, as well as one or more of Aspergillus tubingensis, Aspergillus flavus, Aspergillus oryzae, Aspergillus bombycis and Aspergillus niger.

The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus capable of inhibiting growth of monascus and application of the plant lactobacillus. The preservation number of the plant lactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.34851. According to the present invention, the plant lactobacillus CCNH295 has strong bacterial inhibition ability on common soybean meal contaminating molds, such as aspergillus flavus, aspergillus ochraceus, fusarium moniliforme and penicillium; the plant lactobacillus CCNH295 has excellent bacteriostatic ability to monascus, and when the plant lactobacillus CCNH295 is used for fermentation production of soybean meal, growth of monascus can be remarkably inhibited, so that mildewing of the fermented soybean meal is effectively inhibited, and the prepared fermented soybean meal does not mildew after being placed at 37 DEG C for 6 months; besides, the fermented soybean meal produced by solid state fermentation of the plant lactobacillus CCNH295 is used as a feed raw material, so that the stability of the feed can be improved, and the palatability of the feed can be improved.

The invention discloses a urate oxidase mutant (rAfUOX) with trypsin and chymotrypsin tolerance and application of the urate oxidase mutant (rAfUOX). The invention provides a urate oxidase mutant with trypsin and chymotrypsin tolerance by modifying a key amino acid residue in a wild type aspergillus flavus urate oxidase molecule through a protein engineering technology. The activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after trypsin digestion is increased by 244% compared with the activity half-life period of the wild type rAfUOX, and the activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after chymotrypsin digestion is increased by 191% compared with the activity half-life period of the wild type rAfUOX. Other enzymatic properties are basically consistent with those of the wild type rAfUOX.

The invention discloses a strain of bradyrhizobium guangzhouense APHBXY-23-1 with functions of efficiently promoting growth and strongly inhibiting aspergillus flavus and application of the strain of bradyrhizobium guangzhouense APHBXY-23-1. The preservation number of the strain of bradyrhizobium guangzhouense APHBXY-23-1 is CCTCC (China Center for Type Culture Collection) M 20253048. The invention not only retains the core functions of the strain as rhizobium for efficient symbiotic nitrogen fixation and crop growth promotion, but also reveals that the strain can significantly inhibit the growth of aspergillus flavus by releasing a volatile substance dimethylbutyric acid for the first time. The discovery breaks through the technical limitation that the traditional rhizobium only has a growth promoting function and lacks a biocontrol effect, the two agricultural microorganism core functions of nitrogen fixation and growth promotion and biocontrol bacteriostasis are endogenously integrated into the same microorganism carrier for the first time, and a novel rhizobium resource with double functions is successfully constructed; a new species of growth-promoting rhizobium with a biocontrol function is created, and the growth-promoting rhizobium has important innovative value in theory and application levels.

The application provides an Aspergillus flavus and an enzyme production method and application thereof, and belongs to the technical field of microorganisms.The Aspergillus flavus (Aspergillus flavus) HNZY01 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.42147.The Aspergillus flavus HNZY01 has the ability of high-yield cellulase, can efficiently and synergistically decompose key structural components such as cellulose, hemicellulose and lignin in tobacco stems, and can also increase the content of reducing sugar in the tobacco stems, so that the Aspergillus flavus HNZY01 can be applied in the degradation of the tobacco stems and the smoking safety of cigarettes can be improved.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

The invention relates to a preparation method of a mould-corrosion-resistant ABS electromagnetic protective coating in a humid environment. The preparation method is characterized by comprising the following steps: pretreatment of an ABS matrix, preparation of an electromagnetic shielding layer, preparation of a mould-corrosion-resistant layer and preparation of a hydrophobic layer. In the ABS substrate pretreatment, a diamond free abrasive mechanical polishing method is adopted to remove an oxide layer not exceeding 10 microns on the surface, in the electromagnetic shielding layer preparation, a compact Cu-plated layer is reduced on the surface of the substrate by using an Ag nanoparticle-doped chemical copper plating process, and in the mold corrosion resistant layer preparation, a compact Ni-plated layer is prepared by using a nickel electroplating process on the surface of the electromagnetic shielding layer. And the hydrophobic layer is prepared by injecting a graphene oxide-doped polyurethane layer on the surface of the mould corrosion resistant layer through an injection molding process. According to the invention, 80-100 dB attenuation of broadband (1-18 GHz) electromagnetic waves is realized through the 10-13 [mu] m ultrathin composite coating, the growth of aspergillus niger, aspergillus flavus and other moulds can be inhibited, and the application prospect is wide.

The invention discloses sinorhizium xinjiangense APXJHT24-1 which has the functions of promoting growth and inhibiting aspergillus flavus, and the preservation number of the sinorhizium xinjiangense APXJHT24-1 is CCTCC (China Center for Type Culture Collection) M 2026229. According to the invention, the integration of two major functions of'generation of a characteristic antibacterial volatile matter of dimethyl disulfide 'and'efficient symbiotic nitrogen fixation' on a single strain is realized in Sinkiang Sinkiang rhizobium for the first time. The dimethyl disulfide released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in the aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.

The invention discloses a uricase mutant. The uricase sequence from aspergillus flavus and the uricase sequence from candida are taken as templates, multiple rounds of random mutation are carried out, sequencing verifies that the key amino acid site of the catalytic center is mutated, and the catalytic activity is enhanced. In-vivo and in-vitro uric acid reducing activities of the two uricase mutants are further verified, and the two uricase mutants can significantly reduce the content of uric acid and are expected to become new uricase drugs for treating hyperuricemia.

The invention discloses a calcium-based soil conditioner as well as a preparation method and application thereof, and relates to the technical field of soil improvement. The calcium-based soil conditioner comprises straw, calcium salt, nitrogen-phosphorus-potassium fertilizer and acid-resistant bacillus. Compared with the prior art, after the calcium-based soil conditioner provided by the invention is added into the soil, the abundance of Xanthobacraceae (xanthobacteriaceae) and Xanthomonadaceae (xanthomonas family) in the soil is obviously increased, and meanwhile, the abundance of Aspergillus flavus (aspergillus) and the abundance of Rhizopus arrhzizus (rhizopus arrhizus) in the soil are also obviously reduced.

The present invention discloses a method for constructing a mutant uricase prokaryotic expression engineered bacterium, belonging to the field of genetic engineering technology. The present invention performs four active site mutations on a uricase encoding gene derived from Aspergillus flavus to obtain the uricase encoding gene UOX2. A recombinant expression vector for the UOX2 encoding gene is constructed and introduced into Escherichia coli BL21. The resulting uricase-producing prokaryotic expression engineered bacterium is suitable for industrial batch production, and the specific activity of the produced uricase protein reaches 18.3 U / mg, laying a good foundation for large-scale production of uricase.

This invention discloses a strain of *Burkholderia lagoon* and its application in antagonizing *Aspergillus flavus*, belonging to the field of microbial technology. The *Burkholderia lagoon* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38387. This strain can both inhibit the growth of *Aspergillus flavus* and efficiently degrade aflatoxin B1, thus showing good application prospects and value in the field of food production safety technology.

The invention discloses application of yarrowia caribbica NJC36 in prevention and treatment of aspergillus flavus, and belongs to the technical field of biological prevention and treatment. Experiments prove that the yarrowia caribbica NJC36 can inhibit the growth of aspergillus flavus and the synthesis of aflatoxin, and can be used for preventing and treating kiwi fruits from being infected by the aspergillus flavus. The yarrowia caribbica NJC36 disclosed by the invention has a remarkable prevention and treatment effect on aspergillus flavus, can inhibit the synthesis of aflatoxin, and is environment-friendly.

The invention provides a compound preparation for inhibiting aspergillus flavus and application of the compound preparation in prevention and treatment of aspergillus flavus pollution, and the compound preparation comprises a compound microbial agent and a compound extract preparation, the compound microbial agent is prepared by mixing the following microbial agents in parts by weight: 5-10 parts of bacillus sorola, 5-10 parts of bacillus amyloliquefaciens and 3-8 parts of black yeast; the composite extract preparation is prepared by mixing a winged bean extract and a vanilla bean extract according to a weight ratio of (30-55): 1. The plant extract and the microbial agent are compounded to inhibit the aspergillus flavus, the antibacterial effect is good, and a new direction is provided for preventing and treating the aspergillus flavus of grain and oil crops and grain products.

The invention discloses bacillus velezensis DSHBC4 and application thereof, and belongs to the technical field of microorganisms. The invention aims to provide the bacillus velezensis capable of degrading vomitoxin, aflatoxin or zearalenone and inhibiting the growth of fusarium graminearum and aspergillus flavus. The invention provides bacillus velezensis DSHBC4, the bacillus velezensis is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M20242144, and the preservation time is October 8, 2024. A new solution is provided for biological control of corn crops, and the application potential is wider.

The invention belongs to the technical field of biological mildew preventive, and discloses bacillus licheniformis fermentation liquor and application thereof in mildew prevention and quality improvement of silage. The fermentation liquor is prepared by taking bacillus licheniformis CMCC 63516 as a strain under optimized conditions (with an LB culture medium, the inoculum size of 6%, the liquid loading amount of 30% and culture for 18 hours), and the viable count can reach 1.48 * 10 < 9 > CFU / mL. The strain has a remarkable antagonistic effect on toxin-producing aspergillus flavus, can efficiently degrade aflatoxin B1 (the degradation rate gt in 36 hours is 96%), and has good environmental stability. The additive amount of 1.2-4.0% is applied to corn silage, the growth of mould and saccharomycetes can be remarkably inhibited, the content of AFB1 is greatly reduced, and the mould-proof effect is superior to that of a chemical mould-proof agent sodium diacetate. In addition, the fermentation liquor can also effectively improve the quality of silage, increase the content of crude protein and lactic acid, and reduce the pH and the content of ammoniacal nitrogen and detergent fibers. The invention provides a safe, environment-friendly and efficient biological mildew-proof method which has a wide application prospect in the feed industry.

The application discloses an Aspergillus luchuensis YZ-1 and application thereof, and belongs to the field of microorganism safety.The application separates a new strain from a black tea in Guilin, Guangxi, identifies the strain by ITS, beta tubulin and calmodulin, and the identification result is Aspergillus luchuensis, and the preservation number is CCTCC NO: M 20221462.It is verified by experiments that the strain can inhibit various bacteria and fungi, the water extract and fermentation liquor of the strain can significantly inhibit the growth of Aspergillus flavus, the fermentation liquor has a strong inhibiting effect on the growth of Aspergillus flavus, and the strain has a strong adsorbing effect on aflatoxin B1.Therefore, the Aspergillus luchuensis disclosed by the application has important application value in preventing and controlling mold pollution in grain crops and food.

The invention belongs to the technical field of biocontrol bacteria, and particularly relates to pseudomonas mediterranei with a mildew-proof effect and application of the pseudomonas mediterranei. Specifically, a strain of Pseudomonas mediterranean with a potential biocontrol function is separated from rhizosphere soil of tobacco, and the strain of Pseudomonas mediterranean with the potential biocontrol function is separated from rhizosphere soil of tobacco. The full-length sequence of bacteria is obtained through 16S rRNA gene sequencing, and a phylogenetic tree is constructed to determine the taxonomic status and genetic relationship of the bacteria. A further research shows that the strain not only has a higher self-bacteriostasis rate, but also can inhibit the growth of aspergillus niger, aspergillus flavus, filamentous fungi and talaromyces varioti by generating volatile substances and fermentation products thereof. In addition, the bacteriostatic effect of the strain on the dried tobacco leaves is also verified, and the strain shows good application potential, so that the strain has wide application prospects.

This invention discloses a Litsea cubeba essential oil preparation, which is prepared by mixing a 1% (w / w) chitosan quaternary ammonium salt solution with a Litsea cubeba essential oil ethanol mixture at a volume ratio of 4:1; the volume ratio of Litsea cubeba essential oil to anhydrous ethanol in the Litsea cubeba essential oil ethanol mixture is 2:3. The Litsea cubeba essential oil preparation prepared by this invention exhibits good dispersibility and stability, achieving 100% short-term and long-term antibacterial effect against Aspergillus flavus. This is significantly superior to using Litsea cubeba essential oil or chitosan quaternary ammonium salt alone, as well as other chitosan quaternary ammonium salt-Litsea cubeba essential oil preparations, and even more superior to commonly available antifungal agents. Its preparation method is simple, non-toxic, harmless, and does not pollute the environment. It has low production costs and can be used to prevent or inhibit the growth of Aspergillus flavus, reducing its damage to wooden household products.

The present application provides a mildew inhibitor and its application in tobacco recombination raw materials. The mildew inhibitor mainly comprises clove compound essential oil composed of clove bud essential oil, star anise essential oil and cassia essential oil, and the mass ratio of the clove bud essential oil, the star anise essential oil and the cassia essential oil is 10:(0-4):(0-6). Through tests, the clove compound essential oil can inhibit the growth of various molds such as Aspergillus flavus, Aspergillus niger and Aspergillus montgenieri. The mildew inhibitor has a broad-spectrum inhibitory effect on common Aspergillus of tobacco leaves, can be used as a tobacco mildew inhibitor, can be used for preventing and treating the mildew of tobacco recombination raw materials, and can be in harmony with the tobacco aroma of heated cigarette smoke, so that the mildew inhibition and aroma enhancement effects on the tobacco recombination raw materials are achieved. In addition, as a tobacco mildew prevention additive, the application method of the mildew inhibitor is simple, diverse and adjustable, the integration degree with the core material preparation process is high, and the mold growth inhibition effect is obvious.

The invention discloses bacillus subtilis, a microbial agent and application of the bacillus subtilis, the bacillus subtilis is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC 35166. The invention also provides a preparation method and application of the strain and a storage method of plant seeds. The strain disclosed by the invention has aspergillus flavus resisting activity, and can be used for dressing rice seeds, reducing seed mildewing and promoting seed germination.

This invention relates to the X178 strain, which inhibits fungal diseases in grains and removes mycotoxins, and its applications. The X178 strain belongs to the Nocardiaceae family (…). Nocardiopsis This fungus (sp.) is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 34027. Specifically, it exhibits inhibitory effects against grain pathogenic fungi Aspergillus flavus, Aspergillus niger, and Fusarium graminearum, and can remove two mycotoxins: aflatoxin B1 (AFB1) and / or zearalenone.

The invention discloses a bacillus velezensis ATC-AL and application thereof. The preservation number of the strain is CCTCC (China Center For Type Culture Collection): M20251280. A genome of the bacillus velezensis ATC-AL has a synthetic gene cluster for synthesizing various antifungal active substances, the active substances of the bacillus velezensis ATC-AL can destroy cell surface structures of pathogenic bacteria, induce cell apoptosis and inhibit spore development of pathogenic fungi, and the bacillus velezensis ATC-AL has a broad-spectrum inhibition effect on various pathogenic fungi such as sweet potato coracocystis canaliculata, fusarium graminearum and aspergillus flavus; the generation inhibition rate of vomitoxin reaches 95%, and the generation inhibition rate of aflatoxin B1 can reach 100%; fungus infection experiments on sweet potatoes, corn and wheat prove that active substances in a strain cell fermentation broth can completely inhibit the decay effect of sweet potato coracocystis longirostris, fusarium graminearum and aspergillus flavus on the food crops; according to the invention, a microbial control resource library for biological control of pathogenic fungi and toxin pollution thereof is enriched.