15 results about "Aspergillus flavus" patented technology

The application provides an Aspergillus flavus and an enzyme production method and application thereof, and belongs to the technical field of microorganisms.The Aspergillus flavus (Aspergillus flavus) HNZY01 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.42147.The Aspergillus flavus HNZY01 has the ability of high-yield cellulase, can efficiently and synergistically decompose key structural components such as cellulose, hemicellulose and lignin in tobacco stems, and can also increase the content of reducing sugar in the tobacco stems, so that the Aspergillus flavus HNZY01 can be applied in the degradation of the tobacco stems and the smoking safety of cigarettes can be improved.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

This invention discloses a strain of *Burkholderia lagoon* and its application in antagonizing *Aspergillus flavus*, belonging to the field of microbial technology. The *Burkholderia lagoon* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38387. This strain can both inhibit the growth of *Aspergillus flavus* and efficiently degrade aflatoxin B1, thus showing good application prospects and value in the field of food production safety technology.

This invention discloses a Litsea cubeba essential oil preparation, which is prepared by mixing a 1% (w / w) chitosan quaternary ammonium salt solution with a Litsea cubeba essential oil ethanol mixture at a volume ratio of 4:1; the volume ratio of Litsea cubeba essential oil to anhydrous ethanol in the Litsea cubeba essential oil ethanol mixture is 2:3. The Litsea cubeba essential oil preparation prepared by this invention exhibits good dispersibility and stability, achieving 100% short-term and long-term antibacterial effect against Aspergillus flavus. This is significantly superior to using Litsea cubeba essential oil or chitosan quaternary ammonium salt alone, as well as other chitosan quaternary ammonium salt-Litsea cubeba essential oil preparations, and even more superior to commonly available antifungal agents. Its preparation method is simple, non-toxic, harmless, and does not pollute the environment. It has low production costs and can be used to prevent or inhibit the growth of Aspergillus flavus, reducing its damage to wooden household products.

The present application provides a mildew inhibitor and its application in tobacco recombination raw materials. The mildew inhibitor mainly comprises clove compound essential oil composed of clove bud essential oil, star anise essential oil and cassia essential oil, and the mass ratio of the clove bud essential oil, the star anise essential oil and the cassia essential oil is 10:(0-4):(0-6). Through tests, the clove compound essential oil can inhibit the growth of various molds such as Aspergillus flavus, Aspergillus niger and Aspergillus montgenieri. The mildew inhibitor has a broad-spectrum inhibitory effect on common Aspergillus of tobacco leaves, can be used as a tobacco mildew inhibitor, can be used for preventing and treating the mildew of tobacco recombination raw materials, and can be in harmony with the tobacco aroma of heated cigarette smoke, so that the mildew inhibition and aroma enhancement effects on the tobacco recombination raw materials are achieved. In addition, as a tobacco mildew prevention additive, the application method of the mildew inhibitor is simple, diverse and adjustable, the integration degree with the core material preparation process is high, and the mold growth inhibition effect is obvious.

This invention relates to the X178 strain, which inhibits fungal diseases in grains and removes mycotoxins, and its applications. The X178 strain belongs to the Nocardiaceae family (…). Nocardiopsis This fungus (sp.) is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 34027. Specifically, it exhibits inhibitory effects against grain pathogenic fungi Aspergillus flavus, Aspergillus niger, and Fusarium graminearum, and can remove two mycotoxins: aflatoxin B1 (AFB1) and / or zearalenone.

This invention discloses a method for preparing camphor root volatile oil, which is used for anti-oxidation, anti-aspergillus flavus, and inhibition of aflatoxin synthesis. The camphor root volatile oil of this invention is extracted by steam distillation, with a maximum volatile oil content of 3.34%. Gas chromatography-mass spectrometry analysis of the chemical composition of the camphor root volatile oil confirmed that the main components include camphor, 1,8-cineole, linalool, α-pinene, and sapinene. When applied to inhibit the growth and toxin production of Aspergillus flavus, a volatile oil concentration of 3 μL / mL completely inhibits aflatoxin production. This volatile oil also possesses antioxidant activity, with a half-maximal inhibitory concentration (IC50) for scavenging ABTS free radicals. 50 Below DPPH radicals, its lowest IC50 value is... 50 The values ​​were 0.6115±0.4240 mg / mL and 1.7658±0.2488 mg / mL, respectively. This volatile oil can also inhibit and control Aspergillus flavus contamination in Job's tears, and has application value in the food and health industries.

The application relates to a traditional Chinese medicine and additive compound preparation for treating gizzarditis of laying hens and application, and relates to the technical field of veterinary drugs. The compound preparation is prepared by mixing a traditional Chinese medicine extract and a functional feed additive. The raw material composition of the traditional Chinese medicine extract comprises torrefied malt, torrefied medicated leaven, radix codonopsis, rhizoma atractylodis macrocephalae, rhizoma coptidis, andrographis, dried tangerine or orange peel, white arillus, and liquorice. The additive comprises neutral protease, a mould inhibitor and probiotics. The mould inhibitor is selected from aspergillus flavus B1 degrading enzyme, corn strobukenone degrading enzyme, fumonisin degrading enzyme and vomitoxin degrading enzyme. The probiotics are selected from lactobacillus plantarum, bacillus subtilis and fructo-oligosaccharide. The application is based on the traditional Chinese veterinary theory of food digestion and spleen health, dampness expelling and qi regulation, heat clearing and toxin resolving and mucosa repairing. In combination with the effects of the additive on digestion, mould expelling and intestinal micro-ecological regulation, the application has a remarkable treatment effect on gizzarditis of laying hens, can improve the feed intake and weight gain, reduce the gizzard index, promote the repair of gastric mucosa, and has no antibiotic residue and no toxic side effect.

The application discloses a method for detecting the mildew of soybean meal based on volatile flavor substances, and relates to the technical field of food / feed safety detection, and comprises a soybean meal sample pretreatment step and the following steps: directional enrichment-purification, double-trigger reaction, double-mode detection and result output; in the application, the binary limitation of only judging whether mildew exists or not is broken through temperature-responsive double-ligand affinity microspheres, the aspergillus flavus, aspergillus niger and penicillium are accurately distinguished through an enzyme-antibody double-trigger recognition unit to fill the risk assessment fault, the double-mode detection of graphite powder-carbon black composite conductive hydrogel and indicator takes into account the precision and industrialization cost, the database matching is combined to realize the pollution link traceability and storage environment optimization, and finally, the principle contradiction between the specificity of mold metabolism and the broad spectrum of detection technology is resolved, and the integrated precision identification-risk assessment-targeted prevention and control of soybean meal mildew is achieved.

The application discloses application of 2-ketobutyric acid, and finds that the 2-ketobutyric acid can obviously see the bacteriostatic circle on an aspergillus flavus plate at 2 mg. After the aspergillus flavus is added with 2-ketobutyric acid at 2 mg / mL, and after 24 and 48 hours of culture, the 2-ketobutyric acid can effectively achieve a bacteriostatic rate of more than 90%. Iodine propidium (PI) staining shows that the 2-ketobutyric acid treatment has damage to a fungal cell membrane. Transcriptome analysis shows that the 2-ketobutyric acid can down-regulate gene expression of aspergillus flavus biosynthesis. HPLC analysis can also show that after the aspergillus flavus is treated by the 2-ketobutyric acid, aspergillus flavus is obviously decreased relative to a control group.

The utility model discloses a crop material pile mould-proof device, which relates to the technical field of crop drying and comprises a cart, a heating box is fixedly mounted on one side of the cart, a hot air pipe is fixedly communicated with one side of the heating box, and a recovery mechanism is sleeved on the outer surface of the hot air pipe. According to the ozone drying device, the gas backflow device is arranged, the fan, the heater and the ozone generator are additionally arranged on the base, the Venturi tube is installed at one end of the fixed tube, ozone generated by the ozone generator and hot air in the fixed tube are mixed through the Venturi tube, and the mixed gas is discharged to the drying main tube through the telescopic hose; the air outlet holes in the drying main pipe are used for drying the crop material piles, dried air flows back to the fan through the first air suction holes, used for flowing back to the air outlet holes, in the backflow air pipe and the second air suction holes, used for flowing back to the air outlet holes, in the transverse flow pipe, and the air is recycled; the influence of growth of aspergillus flavus and other similar molds on the crop material pile is prevented, and the mildew-proof effect of the crop material pile is improved.

This invention discloses a strain of *Trichoderma viride* and its application in antibacterial and toxin degradation, belonging to the field of microbial technology. The *Trichoderma viride* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 41619. The *Trichoderma viride* strain exhibits a significant inhibitory effect on the growth and infection of *Aspergillus flavus*, with an inhibitory effect superior to 4000 times dilution of tebuconazole. This *Trichoderma viride* strain can produce enzymes that degrade the cell walls of pathogenic fungi. Fungal cell walls are composed of cellulose, glucan, chitin, and glycoproteins. The chitinase and cellulase produced by *Trichoderma viride* can degrade the fungal cell walls, thereby inhibiting the growth, development, and infection of *Aspergillus flavus*. Furthermore, this invention also experimentally demonstrates that *Trichoderma viride* has a highly efficient degradation effect on aflatoxin and fumonisin.

The application discloses a preparation method of volatile oil of fallen leaves of Cinnamomum camphora, and is used for resisting Fusarium graminearum and inhibiting the synthesis of zearalenone. The volatile oil of the Cinnamomum camphora is extracted by a water vapor distillation method, wherein the content of the volatile oil of the Cinnamomum camphora reaches 3% at most. The chemical components of the volatile oil of the Cinnamomum camphora are analyzed by using a gas chromatography-mass spectrometry combined technique, and it is confirmed that the main components include 1,8 cineole, linalool, alpha-terpineol and hydrate cypressene. The volatile oil of the Cinnamomum camphora is applied to resisting the growth of Aspergillus flavus and toxin production, and when the concentration of the volatile oil is 3.2 muL / mL, the production of zearalenone can be completely inhibited. The volatile oil of the fallen leaves of the Cinnamomum camphora can be applied to food, health care products, medicine and other industries, and various products with antibacterial effects can be manufactured.

This invention provides an Aspergillus flavus strain, its application in extracting zephyranthesin from plant materials, and a method for extracting zephyranthesin from Dioscorea bulbifera. The Aspergillus flavus strain is KS1105. The application of the Aspergillus flavus strain in extracting zephyranthesin from plant materials involves using the strain to ferment and break down the cell walls of plant materials containing zephyranthesin. A method for extracting zephyranthesin from Dioscorea bulbifera includes the following steps: pulverizing Dioscorea bulbifera and mixing it with water; inoculating the mixture with the Aspergillus flavus strain for fermentation and cell wall breaking to obtain broken-wall Dioscorea bulbifera; soaking the broken-wall Dioscorea bulbifera in an alkaline aqueous solution, filtering and washing with water until neutral, and then drying for later use; and extracting the material treated in step S3 with an extractant to obtain a zephyranthesin extract. The Aspergillus flavus strain increases the zephyranthesin extraction rate by 2.48 times compared to before cell wall breaking, reduces the amount of organic solvent used, lowers energy consumption, is environmentally friendly, and has low industrialization costs.