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Bacillus amyloliquefacien for degrading aflatoxin B1 in peanut meal

A technology for dissolving starch spores and aflatoxins, applied in the field of applied microorganisms, can solve the problems of few industrial application studies, limited to the laboratory research stage, etc., and achieve the effects of high industrial application value, increased nutritional value, and convenient operation.

Active Publication Date: 2014-08-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, related research is more limited to the stage of laboratory research, and there are not many researches on industrial application.

Method used

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  • Bacillus amyloliquefacien for degrading aflatoxin B1 in peanut meal
  • Bacillus amyloliquefacien for degrading aflatoxin B1 in peanut meal
  • Bacillus amyloliquefacien for degrading aflatoxin B1 in peanut meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The characteristic of embodiment 1 bacillus amyloliquefaciens CGMCC NO.9021

[0023] (1) Easier to cultivate

[0024] The colony of Bacillus amyloliquefaciens on the broth agar medium is irregular in shape, wrinkled, irregular in edge, off-white, with a raised center, and the colony is sticky. White wrinkle forms when grown in broth. Gram staining was positive, and there were spores in the central part of the bacteria. Cultured in broth medium for 12h can quickly reach 10 9 CFU / mL, and the medium formula is simple, industrial application will be more economical and practical.

[0025] (2) Can inhibit the growth of Aspergillus flavus

[0026] Bacillus amyloliquefaciens significantly inhibits the growth of Aspergillus flavus (such as Aspergillus flavus CGMCC No.3.4408, CGMCC No.3.4410) during the growth process, reducing aflatoxin B from the source 1 produce. Such as figure 1 As shown, Bacillus amyloliquefaciens grows around the filter paper sheet, and the yellow-g...

Embodiment 2

[0033] Example 2 Bacillus amyloliquefaciens CGMCC NO.9021 to low content aflatoxin B in peanut meal 1 Degradation

[0034] Pick the Bacillus amyloliquefaciens on the slant and place them in the broth medium, perform primary activation for 24 hours at 37°C and 200r / min shaking conditions, and then pass through the broth medium containing 3% (m / v) peanut meal for second Grade expansion training for 12 hours. will be contaminated with aflatoxin B 1 The peanut meal sample was pulverized, dried, accurately weighed 50g, added to a 250mL fermentation bottle, and sterilized by high-pressure steam. Add the Bacillus amyloliquefaciens seed solution of the secondary expansion culture to the cooled peanut meal with 10% (v / w) inoculation amount, adjust the ratio of material to water to 1:0.8, stir evenly under sterile conditions, and use it on the bottle mouth 8 layers of gauze wrap. The inoculated fermentation bottle was placed in a constant temperature incubator at 37° C. for 72 hours...

Embodiment 3

[0036] Example 3 Bacillus amyloliquefaciens CGMCC NO.9021 on high content of aflatoxin B in peanut meal 1 Degradation

[0037]Pick the Bacillus amyloliquefaciens on the slant and place them in the broth medium, perform primary activation for 24 hours at 37°C and 200r / min shaking conditions, and then pass through the broth medium containing 3% (m / v) peanut meal for second Grade expansion training for 12 hours. will be contaminated with aflatoxin B 1 The peanut meal sample was pulverized, dried, accurately weighed 50g, added to a 250mL fermentation bottle, and sterilized by high-pressure steam. Add the Bacillus amyloliquefaciens seed solution of the secondary expansion culture into the cooled peanut meal with 10% inoculum amount and 1:0.8 material-to-water ratio, stir evenly under aseptic conditions, and wrap the mouth of the bottle with 8 layers of gauze. The inoculated fermentation bottle was placed in a constant temperature incubator at 37° C. for 72 hours of fermentation,...

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Abstract

The invention discloses a bacillus amyloliquefacien for degrading aflatoxin B1 in peanut meal, and belongs to the technical field of applied microbiology. According to the invention, a bacillus amyloliquefacien capable of significantly inhibiting the growth of Aspergillus flavus and efficiently degrading aflatoxin B1 can be obtained by screening and the content of aflatoxin B1 in detoxified peanut meal is lower than the national limited standard and achieves the safe feeding level after the bacillus amyloliquefacien is applied to moldy peanut meal. Furthermore, the detoxification mechanism of the strain is the degradation effect of extracellular metabolites, and generation of the active substance is of a non-induced type, which is an inherent attribute of the strain. The features of the strain provide the possibility for biocontrol of Aspergillus flavus and aflatoxin B1 in industries such as feed and foods and the strain has very high application values.

Description

technical field [0001] The invention relates to a plant for degrading aflatoxin B in peanut meal 1 The bacillus amyloliquefaciens belongs to the technical field of applied microorganisms. Background technique [0002] Peanut meal is an excellent plant-based protein feed with an annual output of 3 million tons from a wide range of sources; it is rich in protein; its metabolizable energy is also the highest among meal feeds; it also contains 8 kinds of essential amino acids; but peanut meal is very easy to pollute yellow Aspergillus toxin. [0003] Aflatoxins are a class of highly toxic secondary metabolites mainly produced by Aspergillus flavus and Aspergillus parasiticus. In 1993, the International Agency for Research on Cancer (IARC) listed it as a primary carcinogen. Aflatoxin mainly endangers animal and human health through the "three causes" of carcinogenicity, teratogenicity, and mutagenicity, as well as immunosuppression. Its target organ is mainly the liver. Ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L1/015C12R1/07A23L5/20
Inventor 陆健蔡国林徐铭乾
Owner JIANGNAN UNIV
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