37 results about "Aspergillus parasiticus" patented technology

The invention relates to the field of bioengineering, in particular to a preparation method and an application of a microbial agent composition for enhancing secondary fermentation of a bean paste. The microbial agent composition consists of a saccharomycetes agent, an aspergillus oryzae agent and a pleospora agent; and the three microbial agents are prepared, and then the prepared microbial agents are mixed with the secondary-fermented bean paste, so as to promote the secondary fermentation of the bean paste. The whole process of the preparation method of the microbial agent composition provided by the invention is applicable to continuous industrial production; by-products (chili and radix platycodonis), from the production of Pixian bean paste, are comprehensively utilized; a production cycle can be shortened by 6 months, the content of amino nitrogen can be improved by 20% and the content of volatile aroma-producing components can be improved by more than 3 times (the contents of total ester, total acid and total aldehyde); by inoculating aflatoxin B1 with the saccharomycetes agent at a production peak (after being fermented for 30-60 days), an ester producing and aroma generating process can be enhanced, the metabolism of aspergillus flavus and partial aspergillus parasiticus can be competitively inhibited and the content of the aflatoxin B1 can be reduced, and the content of the aflatoxin B1 is lower than 0.5ppm, so that food safety is enhanced.

The invention provides an antifungal peptide and an application thereof to inhibition of generation of aflatoxin. The antifungal peptide has the amino acid sequence as follows: Ala-Asp-Leu-Pro-Val-Lys-Ser-Gly-Asp-Thr-Pro-Glu. The antifungal peptide provided by the invention is capable of effectively inhibiting the growth of aspergillus flavus and aspergillus parasiticus and the generation of aflatoxin, favorable and durable in inhibiting effect and suitable for large-scale production and application.

The invention discloses a method for preparing epipodophyllotoxin diglucoside and a special strain used thereby. The strain is aspergillus parasiticus TEQB with a preservation number of CGMCC No.3116. The strain of the invention can be used for preparing the epipodophyllotoxin diglucoside. The method for preparing the epipodophyllotoxin diglucoside in the invention has the advantages of simple operation, low cost, easy operation and short production period. The method of the invention provides a new way of preparing the epipodophyllotoxin diglucoside, avoids the limitations of other preparation methods in the prior art, has strong operability, is suitable for industrial large-scale production and has a great significant for the protection of the ecological diversity of plants at the same time.

The invention belongs to a classification and identification method upon 12 common fungi, and specifically relates to a method for carrying out classification and identification upon 12 common fungi such as F.graminearum, A.terricola, P.citreonigrum, A.flavus, A.parasiticus, P.aurantiogriseum, T.pseudokoningii, A.humicola, P.viridicatum, A.funigatus, A.uersicolor, and F.solani by using a Fourier transform infrared spectroscopic (FTIR) technology combined with chemometric operation (hierarchical cluster analysis). The method comprises the steps of the establishment of a FTIR spectra information database of the 12 fungi, fungi culturing, ZnSe window manufacturing, spectral acquisition and preprocessing, and result determination by hierarchical cluster analysis. The method has the advantages of high resolution, high speed, simple operation, and low cost.

The invention relates to a serratia plymithica synthetic culture medium and a method for preparing fermentation liquid of the serratia plymithica synthetic culture medium. The formula (gram/liter) of the culture medium comprises the following components of 2 to 10 of colloidalchitin, 3 to 10 of beef extract, 5 to 15 of peptone, 6 to 10 of sodium chloride, 1 to 5 of ammonium sulfate, 1 to 3 of sodium citrate, 1 to 5 of dipotassium phosphate, 0.5 to 3 of magnesium sulfate and 0.01 to 0.05 of ferrous sulfate. The serratia plymithica high-yield fermentation liquid for producing a large quantity of active substances and chitinase which are used for suppressing aflatoxin can be obtained by continuously culturing on a table concentrator at the temperature within 28 and 35 DEG C and the speed of 140 r/min for 4 to 6 days according to the inoculation quantity within 1 to 10 percent. The activity of the chitinase in fermentation supernate is 2.04 to 5.36U/ml; the rate of suppressing the growth of parasitic aspergillus silk is 80.13 to 89.56 percent; and the rate of suppressing the aflatoxin is 90.22 to 98.31 percent. The culture medium is rational in component, and the preparation method is simple and reliable.

The invention relates to a method for synchronously extracting peanut oil and performing high-value utilization to cakes, belonging to the field of deep processing and high-value utilization of peanuts. The processing method comprises the following steps: by taking high-quality peanuts as raw materials, performing high-temperature hot pressing, extracting with edible alcohol, filtering and evaporating, soaking Chinese herbal medicines, performing microbial fermentation, grinding, homogenizing, spray-drying and the like. According to the method, in the step of soaking the Chinese herbal medicines, herba portulacae, herba houttuyniae, fresh rhizoma phragmiti, skullcap, radix astragali seu hedysari, radix glycyrrhizae, acorus calamus and the like are added, and aspergillus parasiticus, aspergillus oryzae, saccharomyces cerevisiae and bacillus are adopted during microbial fermentation. Oil processed by utilizing the method is produced and eaten safely, the price of the peanut cakes is high, the protein solubility is good, the absorption utilization degree is high, the high-nutrition peanut hot-pressed cakes can effectively prevent various bred pest and disease damage as a feed, the cultured animals grow fast, the economic and social benefits are significant, and the high-value utilization of the peanut hot-pressed cakes can be realized.

The invention discloses a preparation and application of a transeliminase encoding gene derived from Aspergillus parasiticus, and an enzyme of the transeliminase encoding gene, that is, a gene of transeliminase is cloned to a pichia pastoris expression vector by using a technical method of gene engineering, then a pichia pastoris recombinant strain for heterologous expression of the enzyme can beobtained, the strain is capable of heterologously expressing the prepared transeliminase and efficiently degrading pectin polysaccharides and polygalacturonic acid (PGA). The transeliminase disclosedby the invention can be widely applied to fields such as agriculture, industry, foods, feed additives, medicines and pectin oligosaccharide preparation.

The present invention discloses an easily digestible feed for pigs and a preparation method thereof. The preparation method comprises the following steps: S1: 20-50 parts by mass of chicken manure granules are mixed with 0.1-2 parts by mass of a microbial fermentation agent to obtain first mixed materials and the microbial fermentation agent is EM (effective microorganisms); S2: 50-200 parts by mass of sweet potato vine leaves and 20-50 parts by mass of radish leaves are taken to be added into the first mixed materials to be mixed to obtain second mixed materials; and S3: the second mixed materials are subjected to an aerobic fermentation for 5-10 days to obtain the easily digestible feed for the pigs. The easily digestible feed for the pigs has a sour scent, can stimulate the pigs to eat, greatly improves the food intake amount, is high in digestibility and nutritional values, contains beneficial live bacteria, and can improve the digestion and absorption functions of the pigs. Lactic acid bacteria can inhibit the growth and toxin produce of molds, lactobacillus acidophilus can inhibit spore germinations of aspergillus parasiticus, and the easily digestible feed for the pigs can promote pig growth.

The invention provides a primer probe combination and a kit for detecting aflatoxin-producing fungi in food, the aflatoxin-producing fungi comprise aspergillus flavus and aspergillus parasiticus, the primer probe combination is characterized in that the primer comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is shown as SEQ ID NO.1, the sequence of the downstream primer is shown as SEQ ID NO.2, the probe is shown as SEQ ID NO.3, the 5' end of the probe sequence is modified with a fluorophore, and the 3' end of the probe sequence is modified with a fluorescence quenching group. The fluorophore is FAM, and the fluorescence quenching group is BHQ1. According to the primer probe combination and the kit provided by the invention, the test method is high in specificity, high in sensitivity, rapid and environment-friendly, and is suitable for rapid detection of main toxin-producing fungi in food in basic laboratories.

The invention relates to a toxigenic medium of AFG2, and an AFG2 toxigenic fermentation method of Aspergillus parasiticus. The medium contains 3-5wt% of yeast extract, and a fermentation medium comprises an enrichment medium and a solid fermentation medium. The toxigenic medium of AFG2 solves the problems of low concentration of the AFG2 in present cereal naturally infecting molds, non-uniformity and long time.

The invention relates to an LAMP primer for detecting Asperillus Parasiticus, and a kit including the primer. The LAMP primer comprises inner primers FIP and BIP, outer primers F3 and B3, and loop primers LF and LB; and the kit comprises the LAMP primer, a Bst DNA polymerase, a reaction buffer solution containing dNTPs, and deionized water. A loop-mediated isothermal amplification technology is used to rapidly detect Asperillus Parasiticus in the invention, so the Asperillus Parasiticus can be rapidly, accurately and conveniently detected, and the LAMP primer and the kit are of great significance to prevent and detect Asperillus Parasiticus pollution. A detection method also disclosed in the invention has the advantages of strong specificity, high sensitivity, lower detection limit reaching 1*10<-12>g/[mu]L, short reaction time, strong operability, no need of expensive apparatuses, convenient product detection, realization through macroscopic observation, and very high practical values.