177 results about "Pentagalacturonic acid" patented technology

A nucleic acid formulation for use in gene delivery comprising a nucleic acid and an anionic polymer is disclosed. Examples of the anionic polymer includes aniionic amino acid polymer or poly-amino acid (such as poly-L-glutamic acid, poly-D-glutamic acid, poly-L-aspartic acid, poly-D-aspartic acid), poly-acrylic acid, polynucleotides, poly galacturonic acid, and poly vinyl sulfate.

The present invention provides nucleotide sequences of Aspegillus fumigatus that encode proteins which exhibit enzyme activities. Vectors, expression constructs, and host cells comprising the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes, and methods for modifying the enzymes in order to improve their desirable characteristics. The activities displayed by the enzymes of the invention include those of a tannase, cellulase, glucose oxidase, glucoamylase, phytase, beta-galactosidases, invertase, lipase, alpha-amylase, laccase, polygalacturonase or xylanase. The enzymes of the invention can be used in a variety of industrial processes. Enzymatically active compositions in various forms as well as antibodies to the enzymes and fragments thereof, are also provided.

A ‘bioactive’ composition that has one or more oligogalacturonans ((1→4)-α-D-galacturonan) or any other oligosaccharides (oligoguluronans) that may present an ‘egg box’ conformation, this conformation being further stabilized by one or more polycationic saccharide(s), preferably either a chitosan oligosaccharide or a chitosan polysaccharide. A method prepares this composition and it is used, in medical, pharmaceutical, agricultural, nutraceutical, food, feed, textile, cosmetic, industrial and / or environmental applications.

Provided are methods of producing 2,5-furandicarboxylic acid (FDCA) from renewable sources such as seaweed, alginate, oligoalginate, pectin, oligopectin, polygalacturonate, galacturonate, and / or oligogalacturonate. The sugars in the renewable sources can be converted into one or more intermediates such as 4-deoxy-L-erythro-5-hexoseulose uronate (DEHU), 4-deoxy-L-threo-5-hexosulose uronate (DTHU), 5-hydroxymethyl furfural (HMF), 2,5-dihydroxymethyl furan (DHMF), and 5-formyl-2-furancarboxylic acid (FFA), which can be converted into FDCA by dehydration and cyclization to produce 5-formyl-2-furancarboxylic acid (FFA), followed by oxidation to produce FDCA. DEHU or DTHU may also be converted into FDCA by oxidation to produce 2,3-dihydroxy-5-oxohexanedioic acid (DOHA), which then undergoes dehydration and cyclization to produce FDCA.

The present invention is directed to methods and compositions for treatment of hyperproliferative diseases. The composition of the invention comprises a carbohydrate having a backbone comprising polygalacturonan and a ligand of peripheral benzodiazepine receptor. The present compositions and methods are used to treat various cancers and other diseases where cells undergo pathological and unwanted proliferation.

The invention provides an enzymolysis treatment method of algae. The method comprises the steps of material preparation, pretreatment, enzyme treatment and centrifugating. In the enzyme treatment step, the adopted enzyme is a composite enzyme which comprises proteinase, cellulase and pectinase in a mass ratio of 3:1:2. The proteinase is any one of papain, bromelin, carboxypeptidase and chymopapain. The cellulase is any one of cellobiohydrolase, endoglucanase, beta-glucosaccharase and cellobiase. The pectinase is any one of protopectinase, lyase and polygalacturonase. According to the enzymolysis treatment method of algae, the extraction rate of sodium alginate is 38.0-50.9%, and the extraction rate of alga glycine is 7.5-11.8%.

The invention discloses alkaline pectinase PelN, as well as encoded gene and an application of the alkaline pectinase PelN. The PelN is protein formed by an amino acid sequence shown as sequence 1 in a sequence table, and has alkaline pectinase activity, and the preservation number of a recombinant bacterium (escherichia coli) BL21 (DE3) PelN containing the encoded gene of the protein in China General Microbiological Culture Collection Center (CGMCC) is CGMCC No. 7166. Experiments prove that the enzyme activity of the protein PelN for degrading polygalacturonic acid is 4590-4950U / mg; the heat stability is better; the relative enzyme activity is above 90% through heat preservation for 120 minutes at 45 DEG C; the optimum pH value of the alkaline pectinase is 9.8; the optimum temperature is 65 DEG C; and production methods of the protein PelN, the recombinant bacterium and the alkaline pectinase provide efficient paths for industrial large-scale production of the alkaline pectinase.

The invention belongs to the field of plant biotechnology, in particular provides a polygalacturonase arrestin gene CaPGIP1 cloned from hot pepper, the gene can specially inhibit the activity of polygalacturonase (PGs) secreted by pathogenic bacteria. The invention provides the function verification techniques based on the coding of the gene, such as protein making, enzyme activity testing, gene silencing, transgenic excessive expression and the like and the application of the techniques. Based on the gene and protein operation technology, the CaPGIP1 gene is proved to effectively participate in the defense reaction of hot pepper under the induction of a stress facto, and can inhibit the activity of polygalacturonase (PGs) secreted by various pathogenic bacteria. The gene silence and transgenic technology are further used for proving that the silence or less expression of the gene in the hot pepper reduces the disease resistance of a host, and the excessive expression of the gene in the transgenic tobacco can enhance the resistance; therefore, the gene codes an important disease-resistance related protein. The invention provides an important technological reserve for transforming the polygalacturonase arrestin (PGIP) gene and cultivating a new disease-resistant variety.

The invention relates to a process for preparing polysaccharide and application of the polysaccharide, in particular to a process for preparing modified pectin (MPHB) with high bioavailability and low molecular weight and application of the modified pectin in the antitumor field. The process comprises the following steps of: mixing pectin and water, heating, and swelling; cooling, regulating the pH value by using carbonate solution, reducing the degree of esterification by using pectinesterase (EC 3.1.1.11), and raising the temperature to inactivate the pectinesterase; and cooling, degrading a main chain of the pectin by using polygalacturonase (EC 3.2.1.15) or pectate lyase (EC 4.2.2.10), and performing membrane filtration on a product, desalting, concentrating and drying to obtain the MPHB. The process is favorable for preparing the MPHB on a large scale and controlling the molecular weight and molecular weight distribution of the MPHB. The MPHB has the activities of resisting livercancer, cervical carcinoma and the like; and the adding of an intestinal absorption enhancer in an oral formula of the MPHB is favorable for further improving the bioavailability.

The invention relates to the field of genetic engineering, specifically to a polygalacturonase mutant with high catalytic efficiency and a preparation method and application thereof. According to the invention, acidic polygalacturonase originated from Penicillium sp. is used as a female parent, and molecular biological techniques are employed for domain replacement and expression of the sequence of the acidic polygalacturonase; under such a condition, specific activity of the acidic polygalacturonase is increased by 11.1 times compared with wild polygalacturonase (before mutation), catalysis efficiency of the acidic polygalacturonase is increased by 33.5 times compared with the wild polygalacturonase (before mutation), and the pH value of an optimal reaction maintains unchanged. Thus, catalysis efficiency of polygalacturonase can be substantially improved, which lays a foundation for application of polygalacturonase in industrial production fields like food, fruit and vegetable processing. The invention is of important guiding significance to improvement of catalysis efficiency of polygalacturonase and other enzymes.

The invention discloses an enteromorpha prolifera oligosaccharide with an auxiliary blood sugar decreasing function and a preparation method of the enteromorpha prolifera oligosaccharide. The preparation method is characterized by comprising the steps of extracting enteromorpha prolifera sulphated polysaccharide by assisting of using microwaves; degreasing, removing protein and pigments; adding polygalacturonase, alpha-L-rhamnosidase and xylanase in sequence to perform enzymolysis; after enzyme denaturation, removing enzymes and undegraded polysaccharide by using ethanol; enabling a centrifugal supernatant to pass through a molecular sieve to obtain a retentate; freezing and drying the retentate to obtain the enteromorpha prolifer aactive oligosaccharide. Verified by pharmacodynamics of animals, enteromorpha prolifera oligosaccharide can remarkably decrease a blood sugar value of a high blood sugar type mouse. Non-specific commercial enzymes are used, the preparation method disclosed by the invention is simple in process route, highly efficient, and environment friendly; the preparation quantity of the oligosaccharide is improved, and the consumption of enzymes and the production cost are reduced; the preparation method is an effective method for preparing the enteromorpha prolifera active oligosaccharide.

The invention belongs to the technical field of biology and in particular provides a polygalacturonase (PG) gene Pcipg5 which is cloned from phytophthora capsici and protein preparation technology thereof. Gene and protein levels prove that the gene is effectively involved in the process that the phytophthora capsici infects hot pepper hosts and results in occurrence of the course of diseases on hot pepper leaves. Plant pathology and cytochemistry technology further prove that after the protein coded by the gene is inoculated onto the hot pepper leaves, obvious withering and shrinking occur on the inoculated parts of the leaves and the cell walls on the affected parts of the leaves are obviously degraded, namely the gene code is an important protein related to the course of diseases or is possibly an important target pathogenic gene of a phytophthora capsici PG gene cluster. The invention provides important technical reserve for further developing phytophthora capsici molecule detection technology.

The present invention provides a process for producing a tea extract, which comprises adding a protease, a tannase and an enzyme preparation having a polygalacturonase activity of 20000 U / g or more to a tea raw material and extracting the desired tea extract from the mixture. According to this process, a tea-leaf-derived cell wall component that cannot be decomposed or extracted by conventional enzymatic tea leaf extraction techniques can be extracted, and a protein of which the extraction becomes possible through the success of the decomposition of the cell wall component can be further decomposed into an amino acid. As a result, it becomes possible to produce a tea extract which contains an amino acid in abundance and is rich in sweet flavor, robust flavor and "umami" (tasty) flavor in high yield.

The invention discloses an alkaline pectinase mutant with improved specific enzyme activity, and belongs to the field of enzyme engineering. Compared with an existing mutant PGL-S1, the specific enzyme activity of the mutant PGL-S1-1 is improved by 5.7 times. Alkaline pectinase can be catalyzed under the alkaline condition, the alpha-1,4 glucosidic bond of polygalacturonic acid is split through the reverse eliminating effect, and the alkaline pectinase mutant can be widely applied to industries such as the food industry, the textile industry and the papermaking industry.

This invention relates to a fungal resistance gene of red skinned pear, namely a polygalacturonase-inhibiting protein gene (PpPGIP), and application. The PpPGIP gene has a base sequence which is stated in a sequence-list SEQ ID; the length of the PpPGIP gene is 1,032bp, wherein 990bp is an open reading frame; and the PpPGIP gene codes polygalacturonase-inhibiting proteins containing 330 amino acids. Functional genomics related technology prove that the PpPGIP gene has the function of improving antifungal function of plants. The antifungal PpPGIP gene is constructed on a plant expression vector and is excessively expressed after being transferred into tobacco, the transgenic tobacco for expressing PpPGIP has strong in vitro antifungal activity and the obvious inhibition effect on a plurality of funguses like Aspergillus niger, Phomopsis funguses, Alternaria alternata, penicillium, and the like.

The invention relates to a molecule detection technique of cayenne pepper phytophthora capsici polygalacturonase (Pcipg) 5 genes, belonging to the field of molecular biology. A pair of specific primers are designed and synthesized by the cayenne (sweet) pepper phytophthora capsici polygalacturonase (Pcipg) 5 genes, the specificity of a plurality of tested bacteria species is verified by PCR, and then the sensitivity of the specific primers is verified by carrying out quantitative molecule detection for a soil cayenne pepper phytophthora oospore and a zoospore; and on the basis, the qualitativeand quantitative molecule detection and the early warning of cayenne pepper phytophthora capsici contained in cayenne pepper diseased field soil, diseased stems, diseased leaves and diseased fruits are carried out. The invention can detect and early warn the cayenne pepper phytophthora capsici in different periods and pathopoiesia characters thereof, thereby being convenient to establish a comprehensive prevention and control technology strategy, determining and selecting an optimal prevention and control period to carry out comprehensive prevention and control and high-efficiency managementfor the cayenne pepper phytophthora capsici, really controlling the infection and the harm of pathogenic bacteria from a source and enhancing the comprehensive prevention and control effect of the cayenne (sweet) pepper phytophthora capsici.

The invention discloses a ramie degumming method. The ramie degumming method includes a), drying and pressing a ramie raw material and b), degumming the pressed ramie by a compound biological enzyme. The compound biological enzyme is formed by mixing a first component with a second component according to the ratio of (70-90): (10-30). The first component is a biological enzyme obtained from fermentation of strains with the preservation number of CGMCC5522. The second component is one or more of polygalacturonase, xylanase, cellulase and glycosidase. Compared with the prior art, the ramie degumming method has the advantages that high degumming rate is achieved and fiber property is enhanced and improved remarkably.

The invention discloses homogeneous pectic polysaccharides and a method for obtaining the same from tea polysaccharides. In particular, the invention relates to polygalacturonic acid homogeneous pectic polysaccharides and a method for obtaining the same from tea polysaccharides or tea leaf polysaccharides. The homogeneous pectic polysaccharides and the method have the following beneficial effects: 1,4-connected polygalacturonic acid pectic polysaccharides are obtained from tea polysaccharides through separation and purification for the first time; the preparation method is simple and is high in yield; acids, alkalies and other toxic and harmful reagents are not used in the whole preparation process, thus the stability of the activity structures of tea polysaccharides can be fully ensured and the preparation method is suitable for large-scale production; in particular, the tea leaves obtained after extracting tea polyphenols can be effectively utilized by using the method, thus the resources can be saved and the refuses can be reclaimed; and the method has great significance in study and production of polygalacturonic acid homogeneous pectic polysaccharides.

The invention relates to a colloid tartro-bismuthate and a drug preparation, a preparation method and application of the colloid tartro-bismuthate. The colloid tartro-bismuthate consists of tartaric acid, trivalent metal bismuth ion oxide or salt and low methoxyl-D-polygalacturonase; and the colloid tartro-bismuthate is subjected to centrifugal spraying and drying by dissolution, mixing, precipitation and other methods to form microcrystalline fine powdery colloid tartro-bismuthate. The drug has obvious treatment effect on chronic nonspecific ulcerative colitis, irritable bowel syndrome and chronic colonic inflammation, has good treatment effect on chronic atrophic gastritis and other stomach diseases, has small toxicity and side effect and belongs to a safe, nontoxic and effective drug of a gastroenterology department.

In some aspects, provided herein are antimicrobial compositions comprising partially esterified polygalacturonic acid and certain fatty acids (e.g., caprylic acid). In some embodiments, the antimicrobial composition may be administered (e.g., topically or orally) to a subject, such as a human patient to treat an infection (e.g., an infection comprising a biofilm). In some aspects, improved catheters are provided.

The invention discloses a method for preparing micro capsulate embedded antihypertensive peptide derived from laver, which comprises: adding laver dry powder and yeast display type polygalacturonase into water, uniformly mixing, reacting at 35 to 37 DEG C for 30 to 45 minutes with stirring, crushing at 28 to 35 DEG C for 1 to 2 hour by ultrasonic waves with power of 730 to 750W, separating and purifying to obtain laver protein; adding laver protein and yeast display type polygalacturonase into water, uniformly mixing, reacting at 35 to 40 DEG C for 2 to 2.5 hours with stirring, separating andpurifying to obtain antihypertensive peptide derived from laver; and embedding the antihypertensive peptide derived from laver by using beta-cyclodextrin. In the invention, yeast display type polygalacturonase and ultrasonic waves are used in combination to make laver protein dissolve out, then the micro capsules of embedded antihypertensive peptide derived from laver are prepared by enzymolysis in presence of yeast display type protease and micro capsule embedding technology, and the obtained antihypertensive peptide derived from laver has high yield and high activity continuity.

The invention relates to the field of genetic engineering, in particular to a high temperature resistant chaetomium polygalacturonase mutant and an encoding gene and an application thereof. Polygalacturonase from achaetomium sp.xz8 is used as a female parent, and the polygalacturonase mutant with improved heat stability is obtained through rational design and molecular biological technique. Related amino acid mutation is combined mutation (ASA, Asp244Ala / Asp299Arg) of two points Asp244Ala and Asp299Arg in each mutant sequence. Optimum temperature, T50 and T1 / 2 of the mutant are improved in comparison with those of wild type enzyme. The optimum temperature is increased from 45 DEG C to 55 DEG C, T50 is increased from 56 DEG C to 73 DEG C, half-life period T1 / 2 at 50 DEG C is increased from 1.5h to 10h, half-life period T1 / 2 at 55 DEG C is increased from 33min to 78min, and Tm value is increased from 43.8 DEG C to 54.1 DEG C. Besides, the mutant keeps enzyme catalytic activity which is equal to that of the wild type enzyme. Application demands in fields of feedstuff, foodstuff and the like can be met.

The invention discloses an alkaline pectinase mutant with improved specific enzyme activity and heat stability, and belongs to the field of enzyme engineering. Compared with an existing mutant PGL-S1, the specific enzyme activity of the mutant PGL-(GS)3-S1 is improved by 6 times and the half-life period at the temperature of 60 DEG C is prolonged by 1.3 times. Alkaline pectinase can be catalyzed under the alkaline condition, the alpha-1,4 glucosidic bond of polygalacturonic acid is split through the reverse eliminating effect, and the alkaline pectinase mutant can be widely applied to industries such as the food industry, the textile industry and the papermaking industry.

The invention discloses a gene sequence of Lygus lucorum polygalacturonase (PG) and a method for preparing the Lygus lucorum polygalacturonase. The method comprises the following steps of: extracting total RNA (ribonucleic acid) of Lygus lucorum; designing a primer according to the conserved sequences of the Lygus lucorum polygalacturonase; amplifying by utilizing an RT-PCR (reverse transcriptase-polymerase chain reaction) method to obtain homologous gene sequences of three PGs; obtaining 5' and 3' unknown sequences by utilizing an RACE experiment; and finally, respectively carrying out the recombinant expression, purification and property analysis of the Lygus lucorum polygalacturonase by utilizing an Escherichia coli expression system and a Pichia pastoris expression system. By using the method, the obtaining problem of the gene sequence of the polygalacturonase of the Lygus lucorum is solved firstly, and the recombinant expression problem of the polygalacturonase derived from insects is also solved for the first time. The gene disclosed by the invention has the application prospect in the aspects of food (fruit juice squeezing), oil material extraction, traditional Chinese medicinal material treatment, paper-making industry, oligo-pectin health care products and the like. In addition, an inhibitor aiming at the Lygus lucorum polygalacturonase can be used as a policy for preventing and controlling a piercing-sucking type pest, thereby achieving the prevention and control on the target pest.

The invention relates to a technology for producing feed-stage pectase by fermenting animal-source penicillium oxalicum obtained by being separated from a goose body, which belongs to the field of microorganism fermentation. An enzyme preparation with high enzyme activity, long storage period, good keeping quality, stable ingredient percentage content and high purity is produced by solid fermentation. The total enzyme activity of the produced pectase enzyme preparation reaches 10123.22U*g; the enzyme activity of polygalacturonic acid is 5101.23U*g which is 50 percent of the total enzyme activity; the pectinesterase activity is 6986.52U*g, which is 69 percent of the total enzyme activity; and the pectin lyase activity is 0.56U*g, which is 0.005 percent of the total enzyme activity. When 0.2 percent of the pectase enzyme preparation is added into the animal daily food, the digesting rate of coarse fiber (CF) is increased by 17.04 percent, the digesting rate of neutral detergent fiber (NDF) is increased by 12.15 percent, the digesting rate of acid detergent fiber (ADF) is increased by 13.95 percent, the net protein utilizing rate (NPU) is increased by 9.53 percent, and the apparent digestibility of Ca and P are respectively increased by 7.04 percent and 8.38 percent than those of a contrast group. The technology solves the key producing technical problem of the unconfirmed enzyme ingredient and the content of the enzyme preparation used for producing the feed of compound bacteria fermentation, and provides a new technology for the reasonable compatibility and application of the enzyme preparation of different ingredients of the pectase.

The invention provides a method for fermenting a Humao tobacco (an important raw material for cigar), which comprises the following steps: a, preparing brown rice cereal juice; b, preparing an activated polygalacturonic acid hydrolase reagent; c, spraying the brown rice cereal juice onto tobacco stalks; d, spraying the polygalacturonic acid hydrolase reagent onto the tobacco stalks; and e, piling the tobacco stalks at normal temperature, covering the tobacco stalks with jute bags to ferment the tobacco stalks, turning the piles when the temperature in the piles reaches 48 to 68 DEG C for the first time, turning the piles when the temperature in the piles reaches 44 to 64 DEG C for the second time, turning the piles when the temperature in the piles reaches 40 to 60 DEG C for the third time, and finishing the fermentation when the temperature in the piles is reduced to 32 to 35 DEG C. By fermenting the tobacco leaves after adding the polygalacturonic acid hydrolase reagent onto the tobacco leaves, the method can improve the fragrance and cleanness of the tobacco leaves and reduce the acrimony of the tobacco leaves, and has the advantages of simple implementation of the steps, readily available raw material and good fermentation effect.

The present invention relates to a multi-functional peptide benefiting expression, purification, stabilization and catalytic efficiency of recombinant proteins, which relates to the field of enzyme engineering and protein purification. The present invention provides a self-assembling amphipathic peptide, which fused with proteins including alkaline polygalacturonate lyase (PGL), lipoxygenase (LOX) and green fluorescent protein (GFP), which leads to successful purification by the nickel affinity chromatography with recovery rates were 47.01%, 39.01% and 56.1%, respectively. Furthermore, the expression quantity and thermostability of the three proteins were enhanced in different degree. Of which the half-life of the PGL-S1v1 and LOX-S1v1 were 2.3 and 3.8-fold as compared with the corresponding wild-type and the specific activity were 1.1 and 1.9-fold increase, respectively. The crude enzyme activity of PGL-S1v1 was 9-fold increase than the PGL.

The invention discloses a strain capable of efficiently expressing alkaline pectinase and an application of the strain and belongs to the technical field of gene engineering. Alkaline pectinase genes are expressed in Pichia pastoris GS115 with a gene recombination technology, and the strain GS115-PIC9K-PGL is obtained and has a remarkably improved yield by comparison with the yield before sequence optimization. According to the strain, the enzyme activity of the alkaline pectinase fermented for 72 h in a shaking flask is 301.9 U / ml and is improved by about 25%, and a good basis is laid for large-scale production of the alkaline pectinase. The alkaline pectinase produced with the strain can catalyze alpha-1,4 glycosidic bonds of polygalacturonic acid to be pyrolysed through trans-elimination under the alkaline condition and is widely applied to food, textile, papermaking and other industries.

The invention provides a biological enzyme preparation processing method of sugar-free dried fruit products. The biological enzyme preparation processing method includes the steps: firstly, hardeningfruit raw materials by the aid of pectinesterase and / or polygalacturonase, and enabling the hardened fruit raw materials to have plasticity to reduce loss of juice in fruits and forming properties ofslices; secondly, softening soaked raw material fruits by the aid of a compound enzyme system of pectinase and cellulose enzyme; thirdly, performing low-temperature inactivation on other early-used enzyme preparations by the aid of protease and / or transglutaminase; finally, performing preservative treatment on dried fruit products by the aid of lysozyme to obtain the sugar-free dried fruit products. Pectic substances in pulp tissues are effectively decomposed, plant cell walls are broken, cell contents are sufficiently released, so that taste is more softened, high-temperature inactivation isreplaced, the cost of high-temperature inactivation is reduced, the water holding capacity of the dried fruit products is improved to some extent, and high-concentration sugar in traditional dried fruits is replaced.