36 results about "Cytochemistry" patented technology

The present invention provides reagents for use in an automated environment for removing or etching embedding media by exposing a biological sample to be stained in histochemical or cytochemical procedures without the dependence on organic solvents. The reagents comprise components optimized to facilitate removal or etching of the embedding media from the biological sample. The present invention also provides reagents for use in an automated environment for cell conditioning biological samples wherein the cells are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The reagents comprise components optimized to facilitate molecular access to cells and cell constituents within the biological sample.

Provided herein are reagents for use in an automated environment for cell conditioning of biological samples wherein the cells or tissues are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The reagents include, but are not limited to, components optimized to facilitate molecular access to cells and cell constituents within the biological sample. Also provided herein are reagents for use in an automated environment for removing or etching embedding media by exposing a biological sample to be stained in histochemical or cytochemical procedures without the dependence on organic solvents. The reagents include, but are not limited to, components optimized to facilitate removal or etching of the embedding media from the biological sample.

The present invention provides devices and associated methods to facilitate multiplexed staining on a slide. The use of multiplexed staining on a single slide allows for more accurate disease diagnosis while also conserving sample. A PDMS based cytology overlay was created that is capable of coupling on-chip morphological, cytochemical, and immunological staining. Morphological and cytochemical staining of blood smears, along with immunostaining of lymph node imprints, was carried out to demonstrate the application of this microfluidic cytology chip (μCC) device.

The invention relates to a P16 immune cell chemical reagent kit and a polypeptide sequence in preparing the same. The kit comprises the following components (1), a first antibody, (2) a second antibody, (3) an enzyme substrate, (4) positive controlled substances, (5) negative controlled substances, and (6) a buffer solution. The kit can be used for marking abnormally expressed P16 protein in human cancer cells and pre-neoplastic cells to allow the cells to develop inordinately so as to distinguish from normal cells or same-type cancer cells where covariation of P16 protein coding gene is not further generated.

The invention discloses a mouse anti-human CD61 monoclonal antibody hybridoma cell line, a monoclonal antibody and a preparation method and application thereof and a flow cytometry detection reagent, and belongs to the technical field of biological engineering. According to the mouse anti-human CD61 monoclonal antibody hybridoma cell line, human peripheral blood leukocytes are used as an immunogen for immunizing Balb / c mice; a cell fusion technology is adopted and hybridoma cells are screened by utilizing an immunocytochemistry and a flow cytometry indirect immunofluorescent method; an antibody generated by monoclonal hybridoma cells is subjected to specific identification by applying an immunoprecipitation-mass-spectrometric method; Western Blot proves that one hybridoma cell line IID5G8 capable of stably secreting a mouse anti-human CD61 monoclonal antibody is obtained and a preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 13300. The monoclonal antibody is prepared by adopting an animal in-vivo induction method; a heavy chain of the antibody is identified to be IgG1 and a light chain is identified to be kappa type; after the antibody is purified, an FITC (Fluorescein Isothiocyanate) label can be used for detecting antigen expression of human peripheral blood platelets CD61 by utilizing flow cytometry direct-labeling immunofluorescence.

The invention relates to the field of liquid-based cytology immunocytochemistry, in particular to an immunohistochemistry kit for auxiliary diagnosis of cervical cancer. The present invention provides a ready-to-use antibody working solution, including antibody reagents, bovine serum albumin, non-reducing sugars, betaine, cyclodextrin, neutral inorganic salts, surfactants, and preservatives; wherein the The antibody reagent is reagent A or reagent B; the reagent A is p16 INK4a The PBS buffer solution of monoclonal antibody and Ki-67 monoclonal antibody; The reagent B is the PBS buffer solution of the goat anti-mouse secondary antibody labeled with horseradish peroxidase and the goat anti-rabbit secondary antibody labeled with alkaline phosphatase; The pH value of the PBS buffer solution is 7.2-7.8.

The invention relates to a device and method for detecting blood leukocytes. Leukocytes play an important role in a human body. Total amount and differential counting of the leukocytes have certain significance in diagnosing various diseases. The conventional method for detecting the leukocytes always combines with two or more detection principles, combines with the techniques, such as, electrics, optics and cytochemistry, and is complex in the detection process. The invention provides the device and method for detecting blood leukocytes in a simple process, for achieving the purpose of analyzing the total amount of the leukocytes. A user can judge if the quantity and size of the leukocytes are abnormal in the manner of adopting laser light for scattering the blood diluted with sheath fluid, collecting the scattering light along the direction at 0 degree and analyzing and comparing the light shadow after scattering.