The invention belongs to the technical field of cellular immunology, and particularly relates to a method for detecting the killing activity of human (gamma)(delta)T cells against a K562 cell strain, which is used for detecting the prepared human (gamma)(delta)T cells. The method comprises the following steps: preparing the human (gamma)(delta)T cells; fetching the K562 cell strains of the logarithmic phase as target cells, and adjusting the cell density to 1*10<5> / ml, 5*10<4> / ml and 2.5*10<4> / ml; paving the target cells in a 96-hole culture plate with 100mu l per hole, and culturing for 24 hours; fetching the prepared human (gamma)(delta)T cells, and adjusting the cell density to 1*10<6>ml; adding the human (gamma)(delta)T cells into the 96-hole culture plate, wherein the effect-target ratios are 10:1, 20:1 and 40:1 respectively; and setting 3 parallel holes for each density, setting blank controls respectively and calculating the killing activity. By adopting the method provided by the invention, the toxicity of the human (gamma)(delta)T cells is guaranteed.