103 results about "Immunomagnetic bead" patented technology

The invention discloses an antibody for detecting indol sulfate, a detection kit and application of the antibody, and the antibody can be specifically combined with indol sulfate and has high combination activity. The detection kit detects the content of indoxyl sulfate in a to-be-detected sample through a competitive binding reaction of an immunomagnetic bead coated indoxyl sulfate antibody, an antigen in the sample and an alkaline phosphatase labeled indoxyl sulfate antigen, and has important economic value and social significance.

The invention provides an immunomagnetic bead, a preparation method and a detection reagent. The preparation method comprises the following steps: preparing a modified magnetic bead containing a dibenzocyclooctyne group; protein and a coupling agent containing azide groups are sequentially dissolved in a second coupling buffer solution to react, modified protein containing azide groups is obtained, the final concentration of the reacted protein is 0.5-5 mg / mL, and the final concentration of the coupling agent is 0.05-5 mM; and mixing the modified magnetic beads and the modified protein according to an addition ratio of 1mg: (1-40) nmol, and reacting to obtain the immunomagnetic beads. According to the present invention, the immunomagnetic bead preparation method has advantages of high coupling efficiency, small inter-batch difference, strong specificity and difficult aggregation during the preparation process, such that the accurate detection of the antigen concentration is achieved so as to solve the defects of low coupling efficiency, large inter-batch difference and easy aggregation existing in the immunomagnetic bead preparation method in the related technology.

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

The invention discloses a plasma or serum exosome separation method which comprises the following steps: firstly, coupling an antibody for resisting four transmembrane protein CD81 on the surface of an exosome by using a magnetic bead to form an immunomagnetic bead; pretreating a serum / plasma sample, enriching exosomes, co-incubating with the immunomagnetic beads, washing the magnetic beads after incubation capture is completed, and washing off uncombined impurities; after being captured, the exosome can be directly cracked, the magnetic beads are removed for downstream experiments, or the magnetic beads are eluted to obtain the complete exosome. The exosome affinity antibody is matched with the magnetic beads, the plasma / serum sample exosome is separated, the unique affinity separation technology not only can remove high-abundance protein in the serum / plasma sample, but also can eliminate other vesicles with similar membrane structures with the exosome, so that the purity of the separated exosome is far higher than that of the exosome obtained by other separation methods.

The present application relates to the technical field of biotechnology, and particularly relates to a D-dimer chemiluminescence assay kit. The present application provides a D-dimer chemiluminescence assay kit, which comprises a magnetic particle suspension (immunomagnetic beads) coated with D-dimer antibody 1, alkaline phosphatase-labeled D-dimer antibody 2 (enzyme-labeled antibody) and a calibrant. The immunomagnetic beads and the enzyme-labeled antibody are prepared by chemical coupling and stored in a buffer containing a soluble high molecular material, which can effectively improve the detection sensitivity and repeatability. The kit can be used in combination with a substrate solution containing a chromogenic substrate such as adamantane and its derivative AMPPD or APS-5 for a full-automatic immune test system, and the content of D-dimer in plasma or whole blood can be determined by a chemiluminescence instrument. The kit has high detection sensitivity and good repeatability.

This invention discloses a microfluidic product plate for screening tumor cells using immunomagnetic beads, belonging to the field of tumor cell screening technology. It includes a dust-free injection-molded or micro-injection-molded product core plate with a pipeline processing mechanism mounted on it for screening target cells in injected liquid. The pipeline processing mechanism includes an input pipe, several delivery pipes, a first connecting pipe, an electromagnetic adsorption unit, a second connecting pipe, and an output pipe. The input pipe is connected to a sample injection pipe, a sheath flow injection pipe, an immunomagnetic bead injection pipe, and a blood cell recovery pipe. The output pipe is connected to a second sheath flow injection pipe, a waste liquid recovery pipe, and a target cell recovery pipe. The first sheath flow injection pipe, the immunomagnetic bead injection pipe, the blood cell recovery pipe, the electromagnet, and the second sheath flow injection pipe work together to separate impurity cells at the front end of the same product core plate, inject and mix immunomagnetic beads in the middle, and precisely sort target cells at the rear end.

The invention relates to the technical field of micro-fluidic detection equipment, and provides an immunomagnetic bead detection system based on a micro-fluidic chip, the immunomagnetic bead detection system comprises a support and the micro-fluidic chip, and a magnetic field device comprises a magnet; the device is characterized in that the magnet is a cylinder magnetized in the diameter direction, the central axis of the magnet is perpendicular to the bottom face of the reaction bin, the diameter of the magnet is larger than the width of the reaction bin, the diameter of the magnet is smaller than the length of the reaction bin, and the diameter of the magnet is larger than or equal to two times of the width of the reaction bin. The magnetic field intensity of the surface of the magnet ranges from 20 mT to 150 mT; meanwhile, the invention also provides a detection method applying the immunomagnetic bead detection system based on the micro-fluidic chip. The magnetic bead detection system has the beneficial effects that the magnetic beads left in the reaction bin after a user washes the magnetic beads are few, the cleaning effect is good, and the detection limit is reduced; the detection method has the advantages of low detection limit and high detection speed.

The invention discloses an immunomagnetic bead with thermomagnetic dual response, a preparation method of the immunomagnetic bead and application of the immunomagnetic bead in detection of food-borne pathogenic bacteria. The preparation method comprises the following steps: (1) preparation of the magnetic core; (2) construction of the inorganic shell layer; (3) introduction of the temperature-sensitive polymer layer; (4) surface functionalization and streptavidin coupling; organic combination of the thermal response polymer and the magnetic core-shell structure is realized for the first time, so that the immunomagnetic bead has dual response capabilities of temperature control gathering and scattering and magnetic field separation. Under a low-temperature condition, the magnetic beads are kept in a dispersed state, so that target combination is facilitated; after the temperature is raised, the polymer is subjected to hydrophobic collapse, particles are gathered, and rapid capture is realized through a magnetic field, so that the detection efficiency and the binding stability are greatly improved.

The present application relates to a kind of human gastric cancer primary fibroblast separation culture and purification method and its application.The human gastric cancer primary fibroblast separation culture and purification method provided by the present application are simple, efficient.The separation culture method and the purification method provided by the present application can complete tissue dissociation in shorter time, and cooperate multiple immunomagnetic beads to remove miscellaneous cells, so that the gastric cancer primary fibroblast group of stable, relatively consistent morphology adherent growth is obtained, phenotype observation is facilitated, and the interference of miscellaneous cells to subsequent function experiment is reduced.Enriched cells are expanded after culture, and can be used for the subtype research of fibroblast, microtumor environment related function experiment.

The invention relates to the field of CAR-T cell concentration detection, in particular to a method for detecting CAR-T cells through combination of microfluidics and immunofluorescence. Through immunomagnetic bead enrichment and micro-fluidic chip partitioning, CAR-T cells are separated from a blood sample through immunomagnetic beads, then labeling is carried out or enhanced by adding a labeling antigen, anti-TCR-PE and DAPI, three-channel fluorescence labeling is achieved, quantitative analysis can be carried out on the number, the cell morphology and the function of the CAR-T cells through automatic immunofluorescence imaging, and the accuracy of CAR-T cell detection is improved. And the detection limit reaches 0.001%.

Provided is a device for screening and separating circulating tumor cells (CTCs), comprising: a chamber module, the chamber module being provided with a mixing chamber, a screening chamber and a waste liquid chamber, and the mixing chamber, the screening chamber and the waste liquid chamber being sequentially communicated; a control module, the control module being mounted on the chamber module, and the control module being configured to control, according to the motion state thereof, whether to discharge a liquid from the mixing chamber to the screening chamber; a temperature control module, the temperature control module being mounted on the chamber module, and the temperature control module being configured to control the temperature in the mixing chamber; and a magnetic enrichment module, the magnetic enrichment module being configured to form a magnetic field in the mixing chamber. Provided is the device for screening and separating CTCs on the basis of immunomagnetic bead separation technology. The device has a compact structure and a small size, is convenient to operate, and has the function of simulating the constant-temperature environment in human bodies to assist in reaction incubation, thereby ensuring the reliability and precision of detection.

The present invention belongs to the field of magnetic separation technology, and in particular, relates to a magnetic separation device and method. The first tooth tip and the tip of the second tooth tip of the magnetic separation device are arranged parallel to each other and form a tip installation gap; a separation channel with an outlet and an inlet is provided on the microfluidic chip; the extension direction of the first tooth tip is arranged parallel to the length direction of the replaceable microfluidic chip and the separation channel; the microfluidic chip is detachably installed in the tip installation gap, and the two opposite outer walls of the microfluidic chip are fitted with the tip of the first tooth tip and the tip of the second tooth tip; when the sample liquid flows into the inlet of the separation channel located in the tip installation gap, the high gradient magnetic field generated between the first tooth tip and the second tooth tip causes the immunomagnetic beads in the sample liquid to be adsorbed on the two opposite inner walls corresponding to the first tooth tip and the second tooth tip in the separation channel. The present invention has a simple structure and production, low cost, and improves the accuracy, separation purity and separation efficiency of magnetic separation.

The application discloses a kind of for visualizing based on immunomagnetic bead detection staphylococcus aureus kit, preparation carboxylated Fe3O4 Nano magnetic beads, the magnetic bead is coupled with anti staphylococcus aureus chicken yolk antibody IgY, and immunomagnetic bead is obtained;Take the liquid to be measured, it is mixed with immunomagnetic bead, is suspended after room temperature on rotating mixer, and is magnetically separated, then appropriate amount of H2O2 Solution is added, and is magnetically separated, and the remaining H2O2 Solution is added 50 μL HRP solution and 50 μL TMB solution color development, and color change of solution can be qualitatively observed with naked eye.The application provides the use of immunomagnetic bead and TMB-HRP-H2O2 color development system combination staphylococcus aureus detection, fast, visual detection staphylococcus aureus, shorten detection time, and the minimum detection concentration is 10 3 CFU·mL ‑1 , with the recovery rate of 102.26%, high sensitivity, good stability, strong specificity.

The application discloses a nucleic acid free extraction reagent of plasma exosome and a direct PCR amplification kit. The nucleic acid free extraction reagent comprises 0.001-0.1% polidocanol and 1-100 mM Tris-HCl in mass volume concentration, and the solvent is water. The direct PCR amplification kit provided by the application comprises the nucleic acid free extraction reagent and an immunomagnetic bead suspension, a washing liquid, a free extraction reagent and a PCR Mix solution; the surface of the immunomagnetic bead is simultaneously coated with three exosome specific antibodies of CD9, CD63 and CD81, so that the recovery rate of the exosome can be greatly improved during the extraction and purification of the plasma exosome; the PCR Mix solution contains an anti-inhibitory component, can effectively neutralize the inhibitors such as proteins in the PCR system, and ensures the amplification efficiency of the PCR. The nucleic acid free extraction reagent of the application can realize the amplification detection of the exosome nucleic acid free extraction, and simplifies the operation process of the PCR detection.

The invention belongs to the technical field of microbiological detection and molecular diagnosis, and discloses a primer group, a kit and a detection method for detecting salmonella enteritidis, the kit comprises an immunocapture component, an isothermal amplification component, a CRISPR / Cas12a detection component and auxiliary components; the isothermal amplification component is any one of an RPA (recombinase polymerase amplification) component or an LAMP (loop-mediated isothermal amplification) component, the RPA component comprises a specific primer group targeting 86th to 212th nucleotides in a salmonella enteritidis hilA gene coding region, and the LAMP component comprises a specific primer group targeting 58th to 250th nucleotides in the salmonella enteritidis hilA gene coding region. The primer group, the kit and the detection method for detecting the salmonella enteritidis successfully integrate three key technologies of RAP and LAMP rapid isothermal amplification, CRISPR / Cas12a high-specificity recognition and trans-cleavage and immunomagnetic bead targeted enrichment, and creatively provide the primer group, the kit and the detection method for detecting the salmonella enteritidis.

The invention discloses a multiple sclerosis antigen-specific regulatory T cell as well as an induction and amplification method and application thereof, and belongs to the technical field of biomedicine. In order to solve the problems that multiple sclerosis (MS) antigen-specific CD4 + regulatory T cells cannot be induced and amplified at present and other antigen-specific CD4 + regulatory T cell induction methods are low in amplification efficiency, low in amplification algebra, low in obtained cell functionality and the like, a B cell activated by sCD40L is used as an antigen presenting cell, and a CD4 + initial T cell sorted by immunomagnetic beads is induced and amplified into Treg; further, the MS antigen specificity Treg is obtained, and a foundation is laid for cell therapy of MS. The induction amplification method provided by the invention has the advantages of rapidness, simplicity, high amplification efficiency, strong functions of obtained cells, high purity after flow sorting and the like, and the obtained MS antigen specific Treg can be used for clinical cell therapy and can be popularized to treatment of other autoimmune diseases.

The invention belongs to the technical field of immune cell detection, and particularly relates to a detection reagent for immune cells and a preparation and detection method of the detection reagent. S2, carrying out enzymolysis digestion; s3, preparing a single-cell suspension; s4, sorting the immunomagnetic beads; s5, carrying out flow dyeing; s6, assembling the kit; according to the immune cell detection reagent and the preparation and detection method, through the synergistic effect of different enzymes, tissues can be decomposed mildly and effectively, the excessive digestion situation possibly caused by only using a certain enzyme is prevented, damage to cell activity is reduced, the activity requirement of cells in subsequent detection is guaranteed, and then the accuracy of a detection result is guaranteed; secondly, various enzymes are used for decomposing different components in the tissue, so that the tissue can be digested into a single-cell suspension more quickly and thoroughly compared with a single enzyme, the cell yield is increased, and enough cells are provided for the subsequent steps of immunomagnetic bead sorting and the like.

The present invention belongs to the field of immunology and detection technology, and specifically relates to a microfluidic chip with a flow channel switching valve. The chip comprises a cleaning liquid flow channel, a buffer flow channel, a waste liquid flow channel, a first mixing flow channel, a second mixing flow channel, a flow channel switching valve, a detection zone, a first waste liquid tank, a second waste liquid tank, a sample well, an immunomagnetic bead injection device, and a fluorescent antibody injection device. The cleaning liquid flow channel, the buffer flow channel, the waste liquid flow channel, the first mixing flow channel, and the second mixing flow channel are respectively connected to the flow channel switching valve, the second end of the second mixing flow channel is connected to the inlet of the detection zone, and the outlet of the detection zone is connected to the second waste liquid tank. The height of the detection zone is less than the spacing between adjacent magnetic bead, sample, and antibody mixed microspheres. Using the microfluidic chip of the present invention for antigen concentration detection can simplify the operating steps of the entire process, improve the efficiency of antigen concentration detection, and greatly save the production cost of the microfluidic chip.

This invention discloses a detection kit for determining prolactin (PRL), comprising immunomagnetic beads, an oxidant, and a surfactant. The oxidant is a peroxidant, and the surfactant is an anionic surfactant. This detection kit for PRL utilizes an oxidant to cleave interfering substances and a surfactant to encapsulate the cleaved substances. This improves the kit's detection sensitivity, enhances its anti-interference ability when testing clinical samples, significantly improves specificity, and greatly increases clinical concordance rate. It solves the problem of falsely elevated results caused by interference from interfering substances during PRL determination.

This invention belongs to the field of biomedical technology and provides a high-throughput drug screening system and its usage method for enriching circulating tumor cells (CTCs) in liver cancer. It includes an information processing and control unit; a diseased liver perfusion device comprising an organ chamber containing injection fluid at the bottom, an arterial supply line, an arterial end sensor, an arterial end clamp valve, at least two membrane lungs, a portal vein supply line, a portal vein end clamp valve, and a portal vein end sensor; a CTC enrichment device, which uses erythrocyte lysis to remove erythrocytes and immunomagnetic bead labeling to remove leukocytes, enabling the processing of large volumes of perfusion fluid in a single step to obtain enriched circulating tumor cells; a high-throughput drug screen; and phototubes, arterial end sensors, and portal vein end sensors, each electrically connected to the information processing and control unit. Compared with existing technologies, the high-throughput drug screening system and its usage method of this invention have the advantages of establishing a room-temperature mechanically perfused ex vivo liver cancer model, realizing the processing of large volumes of liquid specimens, and providing automated high-throughput drug screening functionality.

The invention discloses a method for capturing, identifying and culturing circulating tumor cells of sarcoma in vitro. The method comprises a step of filtering a peripheral blood sample of a sarcoma patient by using a flexible microfiltration membrane, and cells obtained by filtration can be directly subjected to multiplication culture on the filtration membrane. Immunomagnetic beads of a monoclonal antibody combination group coupled with the anti-human leukocyte surface antigen CD45 can be used for negative enrichment and then multiplication culture is carried out in a cell culture bottle, and the captured or cultured cells can be subjected to subtype identification, characterization and counting by adopting an iFISH technology. It is found for the first time that the flexible microfiltration membrane can be used for capturing and culturing the sarcoma circulating tumor cells, and the flexible microfiltration membrane has no toxic effect on the sarcoma circulating tumor cells. The invention not only provides an effective means for real-time monitoring of relapse and metastasis processes of sarcoma patients and chemotherapy drug resistance monitoring, but also can perform drug sensitivity experimental verification on CTC after multiplication culture, and provides a powerful weapon for accurate diagnosis and treatment of sarcoma.

The invention discloses a preparation method and application of immunomagnetic beads based on circulating tumor cell magnetic particle detection, and belongs to the technical field of nanomaterials, the preparation method comprises the following steps: 1) constructing a silicon dioxide magnetic bead skeleton, and 2) performing sulfhydrylation modification on the silicon dioxide magnetic bead skeleton and preparing sulfhydrylated immunomagnetic beads. According to the invention, a stable and dispersed hollow spherical shell structure is synthesized by a strategy of directional self-assembly of anion and cation surfactants. TEOS (tetraethyl orthosilicate) is used as an organic silicon source to prepare a magnetic nano-skeleton, then cysteine is used as the nano-skeleton for sulfhydrylation modification, magnetic beads can be connected to streptavidin through disulfide bonds, the magnetic beads have the capability of being specifically combined with tumor cells, and meanwhile, the magnetism of the magnetic beads can respond in a magnetic field, so that the magnetic beads can be used for detecting tumor cells. And the purpose of sorting the tumor cells is achieved. The immunomagnetic bead provided by the invention not only can separate and enrich circulating tumor cells, but also can meet the requirements of magnetic particle detection, and has the advantages of convenience, real-time performance, high flux and the like.

The present invention relates to an immunomagnetic bead and its application in the separation of renal tubular epithelial cells, belonging to the technical field of immunoassay. The present invention provides an immunomagnetic bead for separating renal tubular epithelial cells in a sample. The immunomagnetic bead includes magnetic beads and antibodies conjugated to the magnetic beads. The antibodies can specifically bind to the surface antigens of renal tubular epithelial cells in the sample to achieve highly efficient capture of renal tubular epithelial cells. Using the immunomagnetic bead to separate renal tubular epithelial cells in the sample has the advantages of low cost, strong specificity, high separation purity, simple operation and high sensitivity. Moreover, the renal tubular epithelial cells sorted by the immunomagnetic bead have a high recovery rate and cell activity, and have little impact on downstream applications. Therefore, the separation of renal tubular epithelial cells in the sample by the immunomagnetic bead has great application prospects in clinical practice.

The invention relates to the technical field of sperm enrichment, in particular to a method for separating and enriching sperms by adopting IgY antibody immunomagnetic beads, which comprises the steps of sample carrier separation and sperm enrichment, and is characterized in that the sample carrier separation comprises the steps of digestion, low-position centrifugation and high-position centrifugation, and the sperm enrichment comprises the steps of immunomagnetic bead combination, magnetic separation and cleaning. The immunomagnetic bead coupled with the IgY antibody has the characteristics of strong specificity, high temperature resistance and acid and alkali resistance, belongs to a food-grade safety reagent, and is low in production cost, complete DNA can be detected only by injecting the immunomagnetic bead coupled with the IgY antibody for immunization of a hen for multiple times, separating and purifying the antibody after regular egg taking, wrapping the immunomagnetic bead coupled with the IgY antibody and tightening sperms, and 30 sperms. Compared with the prior art, an obvious experiment effect is achieved, laboratory operation is more convenient, the immunomagnetic beads can be combined with an instrument to achieve automatic operation, and sperm enrichment operation of mixed spot detection materials is greatly simplified.

The application provides a hidden sperm screening system and method based on magnetic bead capture and fluorescent identification, and is applied to the technical field of magnetic bead fluorescence analysis. A sperm sample is incubated with high-magnetic-saturation first immunomagnetic beads and low-magnetic-saturation second immunomagnetic beads in an incubation module, and the two kinds of magnetic beads are coupled with anti-sperm antibodies and anti-non-sperm cell antibodies respectively. A magnetic field generating module applies an alternating magnetic field to a separation area and synchronously injects sheath liquid, and uses the difference in magnetic response characteristics to retain the complex combined with sperm and to flush away the complex combined with non-sperm cells. A dye introduction module introduces membrane impermeable and permeable dyes under the constraint of a magnetic field, and obtains fluorescent characteristics of membrane-intact and membrane-damaged sperm which can be distinguished. An imaging module performs multi-channel fluorescence imaging, an image analysis module extracts positive events conforming to morphology and fluorescent logic, and finally outputs sperm count parameters and membrane integrity classification parameters synchronously.

The invention provides a neural stem cell induced differentiation and clinical application method for intractable epilepsy, and relates to the technical field of biological medicine, the induced differentiation method comprises the following steps: S1, cell source and preparation: adopting induced pluripotent stem cells from a patient; s2, constructing an induced differentiation system, differentiating the induced pluripotent stem cells into GABA (gamma-aminobutyric acid) inhibitory interneurons, and differentiating the GABA inhibitory interneurons; s3, purifying and amplifying the cells, adopting immunomagnetic bead sorting, and purifying and enriching the cells expressing the GABA energy neuron marker; s4, cell quality control; according to the method, the autologous induced pluripotent stem cells of the patient are taken as a source, immunological rejection is avoided, the transplanting safety is improved, and moreover, the high-purity GABAergic neurons can more effectively supplement damaged inhibitory neurons in the brain of the epileptic and reconstruct the inhibition balance of a neural network, so that the epileptic seizure is more effectively controlled.

The invention relates to the technical field of immunoassay, and discloses an immunomagnetic bead preserving fluid as well as a preparation method and application thereof. The immunomagnetic bead preserving fluid is prepared from the following raw materials: a buffer salt solution, a polymer stabilizer, a surfactant, a chelating agent, inert protein, an antioxidant, a micromolecular protective substance and a preservative. According to the immunomagnetic bead preserving fluid provided by the invention, the preservation time of immunomagnetic beads can be prolonged, so that the immunomagnetic beads can keep good monodispersity, and meanwhile, the activity of active substances such as target proteins can be prolonged, so that the immunomagnetic beads can keep high activity for 24 months under the preservation condition of 4 DEG C; the application effect and product popularization of the immunomagnetic beads in field visual detection are greatly promoted.

The present application relates to the technical field of biological materials, and provides an immunomagnetic bead kit for purifying pedderia acid and a preparation method and application thereof.The immunomagnetic bead kit for purifying pedderia acid comprises immunomagnetic beads formed by coupling carboxylated agarose magnetic beads and pedderia acid monoclonal antibodies, and a buffer preservative solution containing a preservative agent.The present application also provides a preparation method of the immunomagnetic bead kit for purifying pedderia acid.The present application also provides a method for extracting pedderia acid in aquatic products.The present application also provides application of the immunomagnetic bead kit for purifying pedderia acid in enrichment, purification and extraction of pedderia acid.The immunomagnetic bead kit for purifying pedderia acid can be used for enriching and extracting pedderia acid in aquatic products, and has the advantages of high extraction efficiency, simple operation, intelligent and rapid detection, etc.

The invention provides a method for simultaneously determining ochratoxin A and zearalenone in breast milk and application of the method, and belongs to the technical field of food detection. The method comprises the following steps: adding < 13 > C-OTA and < 13 > C-ZEN isotope internal standards into breast milk to be detected, then adding acetonitrile, centrifuging, carrying out purification treatment on supernate by adopting immunomagnetic beads capable of simultaneously purifying ochratoxin A and zearalenone, carrying out nitrogen gas blow-drying and redissolving on the obtained eluent, filtering by virtue of a microfiltration membrane, and collecting the eluent. Detecting by adopting high performance liquid chromatography in series with triple quadrupole mass spectrometry, and substituting a detection result into a linear equation of a standard curve to obtain the concentration of ochratoxin A and zearalenone in the sample to be detected. The method has double functions of enrichment and detection, has the characteristics of high sensitivity and high recovery rate, and provides an efficient and accurate solution for trace pollution detection in breast milk.