60 results about "Immunomagnetic bead" patented technology

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

The present application relates to the technical field of biotechnology, and particularly relates to a D-dimer chemiluminescence assay kit. The present application provides a D-dimer chemiluminescence assay kit, which comprises a magnetic particle suspension (immunomagnetic beads) coated with D-dimer antibody 1, alkaline phosphatase-labeled D-dimer antibody 2 (enzyme-labeled antibody) and a calibrant. The immunomagnetic beads and the enzyme-labeled antibody are prepared by chemical coupling and stored in a buffer containing a soluble high molecular material, which can effectively improve the detection sensitivity and repeatability. The kit can be used in combination with a substrate solution containing a chromogenic substrate such as adamantane and its derivative AMPPD or APS-5 for a full-automatic immune test system, and the content of D-dimer in plasma or whole blood can be determined by a chemiluminescence instrument. The kit has high detection sensitivity and good repeatability.

The invention discloses an immunomagnetic bead with thermomagnetic dual response, a preparation method of the immunomagnetic bead and application of the immunomagnetic bead in detection of food-borne pathogenic bacteria. The preparation method comprises the following steps: (1) preparation of the magnetic core; (2) construction of the inorganic shell layer; (3) introduction of the temperature-sensitive polymer layer; (4) surface functionalization and streptavidin coupling; organic combination of the thermal response polymer and the magnetic core-shell structure is realized for the first time, so that the immunomagnetic bead has dual response capabilities of temperature control gathering and scattering and magnetic field separation. Under a low-temperature condition, the magnetic beads are kept in a dispersed state, so that target combination is facilitated; after the temperature is raised, the polymer is subjected to hydrophobic collapse, particles are gathered, and rapid capture is realized through a magnetic field, so that the detection efficiency and the binding stability are greatly improved.

The present application relates to a kind of human gastric cancer primary fibroblast separation culture and purification method and its application.The human gastric cancer primary fibroblast separation culture and purification method provided by the present application are simple, efficient.The separation culture method and the purification method provided by the present application can complete tissue dissociation in shorter time, and cooperate multiple immunomagnetic beads to remove miscellaneous cells, so that the gastric cancer primary fibroblast group of stable, relatively consistent morphology adherent growth is obtained, phenotype observation is facilitated, and the interference of miscellaneous cells to subsequent function experiment is reduced.Enriched cells are expanded after culture, and can be used for the subtype research of fibroblast, microtumor environment related function experiment.

The invention relates to the field of CAR-T cell concentration detection, in particular to a method for detecting CAR-T cells through combination of microfluidics and immunofluorescence. Through immunomagnetic bead enrichment and micro-fluidic chip partitioning, CAR-T cells are separated from a blood sample through immunomagnetic beads, then labeling is carried out or enhanced by adding a labeling antigen, anti-TCR-PE and DAPI, three-channel fluorescence labeling is achieved, quantitative analysis can be carried out on the number, the cell morphology and the function of the CAR-T cells through automatic immunofluorescence imaging, and the accuracy of CAR-T cell detection is improved. And the detection limit reaches 0.001%.

Provided is a device for screening and separating circulating tumor cells (CTCs), comprising: a chamber module, the chamber module being provided with a mixing chamber, a screening chamber and a waste liquid chamber, and the mixing chamber, the screening chamber and the waste liquid chamber being sequentially communicated; a control module, the control module being mounted on the chamber module, and the control module being configured to control, according to the motion state thereof, whether to discharge a liquid from the mixing chamber to the screening chamber; a temperature control module, the temperature control module being mounted on the chamber module, and the temperature control module being configured to control the temperature in the mixing chamber; and a magnetic enrichment module, the magnetic enrichment module being configured to form a magnetic field in the mixing chamber. Provided is the device for screening and separating CTCs on the basis of immunomagnetic bead separation technology. The device has a compact structure and a small size, is convenient to operate, and has the function of simulating the constant-temperature environment in human bodies to assist in reaction incubation, thereby ensuring the reliability and precision of detection.

The application discloses a nucleic acid free extraction reagent of plasma exosome and a direct PCR amplification kit. The nucleic acid free extraction reagent comprises 0.001-0.1% polidocanol and 1-100 mM Tris-HCl in mass volume concentration, and the solvent is water. The direct PCR amplification kit provided by the application comprises the nucleic acid free extraction reagent and an immunomagnetic bead suspension, a washing liquid, a free extraction reagent and a PCR Mix solution; the surface of the immunomagnetic bead is simultaneously coated with three exosome specific antibodies of CD9, CD63 and CD81, so that the recovery rate of the exosome can be greatly improved during the extraction and purification of the plasma exosome; the PCR Mix solution contains an anti-inhibitory component, can effectively neutralize the inhibitors such as proteins in the PCR system, and ensures the amplification efficiency of the PCR. The nucleic acid free extraction reagent of the application can realize the amplification detection of the exosome nucleic acid free extraction, and simplifies the operation process of the PCR detection.

The invention belongs to the technical field of microbiological detection and molecular diagnosis, and discloses a primer group, a kit and a detection method for detecting salmonella enteritidis, the kit comprises an immunocapture component, an isothermal amplification component, a CRISPR / Cas12a detection component and auxiliary components; the isothermal amplification component is any one of an RPA (recombinase polymerase amplification) component or an LAMP (loop-mediated isothermal amplification) component, the RPA component comprises a specific primer group targeting 86th to 212th nucleotides in a salmonella enteritidis hilA gene coding region, and the LAMP component comprises a specific primer group targeting 58th to 250th nucleotides in the salmonella enteritidis hilA gene coding region. The primer group, the kit and the detection method for detecting the salmonella enteritidis successfully integrate three key technologies of RAP and LAMP rapid isothermal amplification, CRISPR / Cas12a high-specificity recognition and trans-cleavage and immunomagnetic bead targeted enrichment, and creatively provide the primer group, the kit and the detection method for detecting the salmonella enteritidis.

This invention discloses a detection kit for determining prolactin (PRL), comprising immunomagnetic beads, an oxidant, and a surfactant. The oxidant is a peroxidant, and the surfactant is an anionic surfactant. This detection kit for PRL utilizes an oxidant to cleave interfering substances and a surfactant to encapsulate the cleaved substances. This improves the kit's detection sensitivity, enhances its anti-interference ability when testing clinical samples, significantly improves specificity, and greatly increases clinical concordance rate. It solves the problem of falsely elevated results caused by interference from interfering substances during PRL determination.

This invention belongs to the field of biomedical technology and provides a high-throughput drug screening system and its usage method for enriching circulating tumor cells (CTCs) in liver cancer. It includes an information processing and control unit; a diseased liver perfusion device comprising an organ chamber containing injection fluid at the bottom, an arterial supply line, an arterial end sensor, an arterial end clamp valve, at least two membrane lungs, a portal vein supply line, a portal vein end clamp valve, and a portal vein end sensor; a CTC enrichment device, which uses erythrocyte lysis to remove erythrocytes and immunomagnetic bead labeling to remove leukocytes, enabling the processing of large volumes of perfusion fluid in a single step to obtain enriched circulating tumor cells; a high-throughput drug screen; and phototubes, arterial end sensors, and portal vein end sensors, each electrically connected to the information processing and control unit. Compared with existing technologies, the high-throughput drug screening system and its usage method of this invention have the advantages of establishing a room-temperature mechanically perfused ex vivo liver cancer model, realizing the processing of large volumes of liquid specimens, and providing automated high-throughput drug screening functionality.

The invention discloses a method for capturing, identifying and culturing circulating tumor cells of sarcoma in vitro. The method comprises a step of filtering a peripheral blood sample of a sarcoma patient by using a flexible microfiltration membrane, and cells obtained by filtration can be directly subjected to multiplication culture on the filtration membrane. Immunomagnetic beads of a monoclonal antibody combination group coupled with the anti-human leukocyte surface antigen CD45 can be used for negative enrichment and then multiplication culture is carried out in a cell culture bottle, and the captured or cultured cells can be subjected to subtype identification, characterization and counting by adopting an iFISH technology. It is found for the first time that the flexible microfiltration membrane can be used for capturing and culturing the sarcoma circulating tumor cells, and the flexible microfiltration membrane has no toxic effect on the sarcoma circulating tumor cells. The invention not only provides an effective means for real-time monitoring of relapse and metastasis processes of sarcoma patients and chemotherapy drug resistance monitoring, but also can perform drug sensitivity experimental verification on CTC after multiplication culture, and provides a powerful weapon for accurate diagnosis and treatment of sarcoma.

The invention provides a neural stem cell induced differentiation and clinical application method for intractable epilepsy, and relates to the technical field of biological medicine, the induced differentiation method comprises the following steps: S1, cell source and preparation: adopting induced pluripotent stem cells from a patient; s2, constructing an induced differentiation system, differentiating the induced pluripotent stem cells into GABA (gamma-aminobutyric acid) inhibitory interneurons, and differentiating the GABA inhibitory interneurons; s3, purifying and amplifying the cells, adopting immunomagnetic bead sorting, and purifying and enriching the cells expressing the GABA energy neuron marker; s4, cell quality control; according to the method, the autologous induced pluripotent stem cells of the patient are taken as a source, immunological rejection is avoided, the transplanting safety is improved, and moreover, the high-purity GABAergic neurons can more effectively supplement damaged inhibitory neurons in the brain of the epileptic and reconstruct the inhibition balance of a neural network, so that the epileptic seizure is more effectively controlled.

The present application relates to the technical field of biological materials, and provides an immunomagnetic bead kit for purifying pedderia acid and a preparation method and application thereof.The immunomagnetic bead kit for purifying pedderia acid comprises immunomagnetic beads formed by coupling carboxylated agarose magnetic beads and pedderia acid monoclonal antibodies, and a buffer preservative solution containing a preservative agent.The present application also provides a preparation method of the immunomagnetic bead kit for purifying pedderia acid.The present application also provides a method for extracting pedderia acid in aquatic products.The present application also provides application of the immunomagnetic bead kit for purifying pedderia acid in enrichment, purification and extraction of pedderia acid.The immunomagnetic bead kit for purifying pedderia acid can be used for enriching and extracting pedderia acid in aquatic products, and has the advantages of high extraction efficiency, simple operation, intelligent and rapid detection, etc.

The invention provides a method for simultaneously determining ochratoxin A and zearalenone in breast milk and application of the method, and belongs to the technical field of food detection. The method comprises the following steps: adding < 13 > C-OTA and < 13 > C-ZEN isotope internal standards into breast milk to be detected, then adding acetonitrile, centrifuging, carrying out purification treatment on supernate by adopting immunomagnetic beads capable of simultaneously purifying ochratoxin A and zearalenone, carrying out nitrogen gas blow-drying and redissolving on the obtained eluent, filtering by virtue of a microfiltration membrane, and collecting the eluent. Detecting by adopting high performance liquid chromatography in series with triple quadrupole mass spectrometry, and substituting a detection result into a linear equation of a standard curve to obtain the concentration of ochratoxin A and zearalenone in the sample to be detected. The method has double functions of enrichment and detection, has the characteristics of high sensitivity and high recovery rate, and provides an efficient and accurate solution for trace pollution detection in breast milk.

The invention relates to the field of immunosensors, and discloses a preparation method of a double-signal intelligent immunosensor and application of the double-signal intelligent immunosensor in silk fibroin detection. The preparation method comprises the following steps: (1) preparing the INV / HRP-ZIF-90 nanoparticles; (2) preparing an antibody functionalized magnetic bead probe solution; (3) preparing an Ab2-INV / HRP (at) ZIF-90 solution; and (4) preparing the double-signal intelligent immunosensor. According to the double-signal immunosensor, on the basis of an immunomagnetic bead ZIF-90 sandwich structure, ATP is used for triggering release of double enzymes (sucrose invertase and horse radish peroxidase), and a double-mode detection means of glucometer signal conversion and TMB color development-Image J analysis is combined, so that qualitative and semi-quantitative detection of a target antigen in silk can be realized, double signals can be mutually verified, and the detection sensitivity is high. And the detection accuracy is improved. The double-signal immunosensor not only has strong response specificity, but also has good portability, and can meet the requirements of on-site rapid detection and result verification of silk cultural relics.

The utility model provides a microfluidic chip for skin cell sorting, which relates to the technical field of cell sorting and comprises a substrate, a microfluidic channel is arranged on the upper surface of the substrate, and the microfluidic channel comprises a primary sorting channel and two secondary sorting channels. The first-stage sorting channel is composed of three sorting matrixes, the second-stage sorting channel is composed of two sorting matrixes, and each sorting matrix is formed by connecting an expansion area with a contraction area. According to the skin cell sorting device, an external magnetic field acts on the immunomagnetic beads and combined cells, so that the sorting efficiency is greatly improved under the condition of effectively sorting the skin cells. Moreover, corollary equipment is simplified and only relates to the upright microscope and the micro-injection pump, so that the operation of the micro-fluidic chip is simpler and more convenient, and the use cost is remarkably reduced.

The application discloses a method for detecting immune killing activity of whole blood without cell separation, and belongs to the technical field of in-vitro immune detection. The method directly adopts fresh peripheral blood anticoagulated by heparin, does not need density gradient centrifugation, immune magnetic bead sorting or any immune cell purification step, and directly obtains the specific killing rate of whole blood immune cells to target cells through cytokine pre-activation, co-culture of fluorescently labeled target cells, apoptosis staining and flow cytometry analysis. The application has the advantages of simple operation, short time consumption, complete cell activity reservation, high physiological correlation, good repeatability and the like, and can truly reflect the comprehensive killing function of whole blood immune cells.

The invention provides a peripheral blood NK cell efficient amplification method which comprises the following steps: collecting peripheral blood and separating mononuclear cells, removing CD3 + lymphocytes by an immunomagnetic bead method to obtain initial NK cells, coating a culture plate with an anti-NKp46 antibody, performing first-stage activation under the combination of IL-2 and IL-12, and co-culturing with inactivated trophoblast cells to obtain the NK cells. Second-stage amplification is completed in a culture medium containing IL-15, IL-21, IL-33, NAC, melatonin and other additives, and finally, CD3-CD56 + cells are obtained through flow sorting; according to the method, the NK cell amplification multiple is larger than or equal to 1000 times, the purity is maintained to be 91% or above, the cell activity and killing activity are high, and an efficient and feasible scheme is provided for clinical cell therapy.

The application relates to the technical field of immunomagnetic bead preparation, in particular to a composite antibody immunomagnetic bead with a ring-shaped magnetic attraction structure and a preparation method and application thereof. After being cleaned and activated, the magnetic bead is coupled with at least two kinds of composite anti-red blood cell antibodies with different antigen epitopes, and after being sealed, free anti-red blood cell antibodies are added to prepare a vacuum freeze-dried product; through a matched ring-shaped magnetic attraction structure, a plurality of trapezoidal permanent magnets are assembled according to a Halbach array, the periphery is fixed through a ring-shaped stainless steel sleeve, and a uniform radiation ring-shaped magnetic field is formed; when applied, a mixed solution of whole blood and the magnetic bead is placed in the center hole of the structure and is magnetically attracted for 10-90 seconds; the composite antibody immunomagnetic bead with the ring-shaped magnetic attraction structure and the preparation method and application thereof can significantly improve the red blood cell capturing and separation efficiency, shorten the separation time, reduce the magnetic bead consumption, facilitate plasma suction, cover most clinical scenes, and reduce the industrialization cost.

The invention discloses a multi-antibody immunomagnetic bead as well as a preparation method and application thereof, and relates to the technical field of immunomagnetic beads and chemical pollutant detection. The multiple antibody immunomagnetic beads comprise a sulfadimidine immunomagnetic bead, an enrofloxacin immunomagnetic bead, an aflatoxin M1 immunomagnetic bead, a tetracycline immunomagnetic bead and a melamine immunomagnetic bead, and each immunomagnetic bead is obtained by coupling a sulfadimidine antibody, an enrofloxacin antibody, an aflatoxin M1 antibody, a tetracycline antibody and a melamine antibody with magnetic microspheres; the invention also provides application of the multi-antibody immunomagnetic beads in simultaneous detection of multiple chemical pollutants in animal-derived food, and a kit and a detection method for simultaneous detection of multiple chemical pollutants in animal-derived food. The method has the beneficial effects that the method can be used for simultaneously detecting various chemical pollutants in the animal-derived food, and is high in detection efficiency and good in specificity.

The present application relates to the field of immunodetection technology, and particularly relates to a method for enriching and detecting antigen-specific T cells. The method for enriching antigen-specific T cells comprises the following steps: stimulating a whole blood sample with a specific antigen, and then sorting T cells in the whole blood sample stimulated by the antigen by using an immunomagnetic bead sorting technology to obtain a T cell sample. The method is based on the strategy of stimulating the antigen first and then sorting the cells, and can quickly and accurately separate T cells from a complex cell system of whole blood, effectively avoiding the complicated operation of traditional sorting methods, realizing automatic sorting, simplifying the operation steps, shortening the sorting time, improving the sorting throughput, and better ensuring the accuracy of subsequent cell detection. The T cells obtained by the above enrichment method can be detected by using an ELISPOT method, and the antigen-specific T cells can be accurately detected.

The invention discloses a gastric cancer biomarker ENO3 and a detection reagent thereof, belongs to the technical field of biological medicines, and particularly relates to a detection reagent of a gastric cancer marker. The detection reagent comprises an upstream primer and a downstream primer of a gastric cancer marker, and modified immunomagnetic beads, the gastric cancer marker is enolase 3. The invention finds and verifies that the gastric cancer marker enolase 3 promotes malignant transformation of gastric cancer by promoting glycolysis of gastric cancer cells, activating a WNT / beta-catenin signal channel and up-regulating expression of matrix metalloproteinase at the same time; meanwhile, by optimizing an immunomagnetic bead structure targeting the marker, the capture efficiency of the marker on trace tumor cells in peripheral blood is improved, so that the signal intensity of subsequent marker detection is enhanced, and an important tool is provided for improving the positive detection rate of gastric cancer detection based on the peripheral blood.

The invention relates to the technical field of biomedicine, in particular to an automatic analysis and risk assessment system and method for peripheral blood endothelial progenitor cells, and the method comprises the following steps: collecting a peripheral blood sample and clinical data of a subject; the method comprises the following steps: enriching CD34 + cells from a peripheral blood sample based on microfluidic-immunomagnetic bead enrichment, carrying out VEGFR2 and DAPI immunofluorescent staining on the enriched cells, and obtaining a multi-channel fluorescent digital image; analyzing the multi-channel fluorescent digital image, identifying and counting VEGFR < 2 + > and DAPI < + > double-positive cells to obtain an EPC counting result; inputting the EPC counting result and clinical data of the subject into a multi-modal AI risk assessment model to obtain a risk assessment result; the health management suggestion information is formulated based on the risk assessment result, so that the model can have better suitability with the target subject, and the accuracy of risk assessment is improved.

The application belongs to the technical field of biological detection, and particularly relates to an immunomagnetic bead for chemiluminescence immunoassay and a preparation method and application thereof. The immunomagnetic bead is of a core-shell structure, and comprises a magnetic core, an epoxy shell layer, a hydrophilic spacer wall, a boronic acid group coupling layer and an antibody. The magnetic core is prepared by using a solvothermal method, the epoxy polymer shell layer is prepared by using a backflow precipitation polymerization, the hydrophilic PEG long chain is modified by using click chemistry reaction of the double-end thiol-containing PEG molecule and the epoxy group, and finally the boronic acid group is fixed on the surface of the microsphere by thiol-double bond click chemistry reaction to obtain the magnetic composite microsphere. The antibody is coupled to the surface of the magnetic bead carrier by coordination bonding of the cis-diol group in the structure and the boronic acid group on the surface of the microsphere to obtain the chemiluminescence immunomagnetic bead. The immunomagnetic bead can be applied to detection of tumor markers, has the advantages of simple synthesis route, easy operation, high antibody coupling efficiency, high detection sensitivity and strong specificity, and can realize efficient immunoassay.

The invention belongs to the technical field of gene detection, and provides a method for separating, enriching and rapidly detecting Listeria monocytogenes, which comprises the following steps: S1, preparing immunomagnetic beads labeled with an anti-Listeria monocytogenes monoclonal antibody 2F9; s2, mixing the immunomagnetic beads obtained in the step S1 with a sample to be detected, and enriching listeria monocytogenes through a magnetic separation technology; s3, preparing a rapid detection kit; s4, detecting the enriched listeria monocytogenes by using the rapid detection kit in the step S3; by preparing the immunomagnetic beads labeled with the listeria monocytogenes monoclonal antibody 2F9, specific separation and enrichment of listeria monocytogenes in a sample are realized, and the problems of high target strain acquisition difficulty and long culture period in enrichment of a traditional culture method are solved; meanwhile, the defect that an existing gene detection method cannot specifically separate and enrich target bacteria is overcome, and the detection pertinence and efficiency are greatly improved.

Analysis of cell populations for both research and clinical applications such as cell therapy is often performed on whole blood that is close to 24 hours old. The reason for this is that the analysis site is removed from the blood draw site so that blood must be shipped overnight to the analysis site. During the 24-hour shipping period, granulocytes and other blood components break down into undesirable cell debris including nucleic acids that are released into the blood. Such debris is known to interfere with immunological assays and with thawing following freezing of samples. The present invention solves this issue by removing granulocytes (and optionally other components) using an anti-CD15 molecule (or other appropriate molecule) bound to metallic magnetic particles. Following mixing, the granulocytes (or other components) are removed and prepared for shipment to the analysis site or for other reasons.

The utility model discloses a multiplex fluorescence immunomagnetic bead separation reagent card, and relates to the technical field of biological detection. The reagent card comprises an upper cover and a shell, wherein the upper cover is provided with a sample adding hole; the lower part of the sample adding hole is connected with a first microfluidic pipeline arranged in the shell; a reaction bin, a detection bin, a quality control bin, a first capturing bin and a second capturing bin are also arranged in the shell; the first microfluidic pipeline is communicated with the reaction bin; the reaction bin connects the quality control bin and the first capture bin in series through a second microfluidic pipeline; the reaction bin connects the detection bin and the second capture bin in series through a third microfluidic pipeline; each detection bin is communicated with the corresponding second capture bin through an independent third microfluidic pipeline; and magnetic strips are arranged in the first capturing bin and the second capturing bin. According to the scheme, a to-be-detected sample only needs to be dropwise added into the sample adding hole, filtering treatment, mixing, reaction and multi-item detection of the sample can be achieved, different target objects are shunted through the independent third microfluidic pipeline, and the detection sensitivity is improved.

The invention relates to a method for separating and enriching polystyrene micro-nano plastic, and belongs to the technical field of analysis and detection. The method comprises the following steps: firstly, combining carboxyl magnetic beads or Protein A magnetic beads with a polystyrene polyclonal antibody to obtain immunomagnetic beads; then taking a liquid sample containing the polystyrene micro-nano plastic, adding immunomagnetic beads, uniformly mixing the immunomagnetic beads and the sample, incubating, performing magnetic field separation, removing supernate, and removing the magnetic field to obtain the immunomagnetic bead compound separated and enriched with the polystyrene micro-nano plastic. According to the method, the polystyrene micro-nano plastic is subjected to specific separation and enrichment by adopting an immunomagnetic separation technology, and the surfaces of superparamagnetic microspheres are coupled with polystyrene micro-nano plastic specific antibodies to prepare immunomagnetic beads, so that the polystyrene micro-nano plastic in a to-be-detected sample is subjected to specific recognition and capture. The immune complex is separated and enriched under a certain magnetic field condition to form an immunomagnetic bead and micro-nano plastic complex, so that rapid separation of a polystyrene micro-nano plastic target object is realized.

The invention provides a method for simultaneously and rapidly detecting vibrio parahaemolyticus and multiple drug-resistant genes thereof, which comprises the following steps: preparing vibrio parahaemolyticus immunomagnetic beads by coupling biotinylated vibrio parahaemolyticus polyclonal antibodies with streptavidin nano magnetic beads, and carrying out immunocapture on the vibrio parahaemolyticus. According to the present invention, the RPA-coupled micro-fluidic detection system is designed, the capture condition and the nucleic acid extraction process are optimized, the reasonable RPA-coupled micro-fluidic detection system is designed, the toxR gene of the vibrio parahaemolyticus and the three most common drug-resistant genes floR, sul1 and qnrA are synchronously detected, and the important significance is provided for solving the drug resistance problem of marine product pathogenic microorganisms and ensuring the human health.