200 results about "Immunomagnetic bead" patented technology

The utility model discloses a full-automatic whole blood sample pretreatment device for separating plasma through immunomagnetic beads. The full-automatic whole blood sample pretreatment device comprises a plasma separation mechanism for extracting a plasma sample from a whole blood sample, a conveying mechanism for conveying a blood collection tube and a sample cup, and a feeding mechanism for providing the sample cup, the plasma separation mechanism comprises a sample loading and shaking module for uniformly mixing a whole blood sample in the blood collection tube, a puncture sampling module for collecting the whole blood sample or the plasma sample, and a magnetic separation module for separating the plasma sample; the magnetic separation module comprises an oscillation assembly, a magnetic attraction assembly and a reaction cup, and freeze-dried immunomagnetic beads and an antibody reagent are pre-stored in the reaction cup. The device provided by the utility model can be used for comprehensively and automatically pretreating a whole blood sample and improving the quality and the efficiency of plasma separation.

The invention discloses a preparation method of sandwich type immunomagnetic beads with high thermal stability, which comprises the following steps: by taking polystyrene microspheres as a template, introducing oil-phase Fe3O4 nanoparticles into the polystyrene microspheres through a swelling method, and then carrying out hydrolytic condensation reaction on the surface of a microsphere assembly by utilizing n-octyltrimethoxysilane OTMS to obtain the sandwich type immunomagnetic beads with high thermal stability. The preparation method comprises the following steps: carrying out surface amination modification on a silicon dioxide thin layer which is grown by hydrolysis of tetraethoxysilane TEOS to realize coating of an outermost organic shell layer, carrying out surface amination modification, and carrying out cross-linking on 1, 4-butanediol diglycidyl ether and erythritol on the surface to form an epoxy polymer layer so as to obtain the sandwich-type immunomagnetic bead with a cross-linked epoxy polymer layer introduced to the outer layer. According to the epoxy group modification process on the surface of the magnetic bead, a direct cross-linking modification process is adopted, so that the operation steps are simplified, and the obtained epoxy group magnetic bead is ensured to show excellent biocompatibility. In addition, the method omits the tedious process of functional group activation, and allows direct coupling with the antibody, thereby greatly improving the efficiency and convenience of the experiment.

The invention provides a circulating tumor cell screening and separating device and a circulating tumor cell detection method, and belongs to the technical field of medical detection.The circulating tumor cell screening and separating device comprises a chamber module, the chamber module is provided with a mixing chamber, a screening chamber and a waste liquid chamber, and the mixing chamber, the screening chamber and the waste liquid chamber are sequentially communicated; the control module is mounted on the cavity module, and the control module is arranged to be capable of controlling whether the liquid is discharged from the mixing cavity to the screening cavity or not according to the motion state of the control module; the temperature control module is installed in the cavity module, and the temperature control module is arranged to be capable of controlling the temperature in the mixing cavity; the magnetic enrichment module is arranged to be capable of forming a magnetic field in the mixing cavity; the CTCs screening and separating device has the advantages that the CTCs screening and separating device based on the immunomagnetic bead separation technology is provided, the device is compact in structure, small in size and convenient to operate, the function of simulating the human body constant-temperature environment to assist in reaction cultivation is achieved, and the reliability and the detection precision during detection are ensured.

The invention discloses a magnetic bead for eliminating an anti-CD47 monoclonal antibody drug, a preparation method of a reagent and a detection method, the immunomagnetic bead comprises a carboxyl magnetic bead located in the immunomagnetic bead and a human CD47 recombinant protein wrapping the periphery of the carboxyl magnetic bead, and the particle size of the immunomagnetic bead is 100 nanometers; according to the method, only one round of adsorption is needed, the serum magnetic bead mixed solution can be placed on the magnetic frame for magnetic separation after full vortex mixing, interference can be completely removed, the time is very short, and detection can be completed within 1 min.

The invention provides a method for detecting salmonella enteritidis, which comprises the following steps of: 1, expressing a nano antibody for resisting salmonella enteritidis FliC by using escherichia coli, and verifying the nano antibody; step 2, preparing immunomagnetic beads for resisting FliC protein, and verifying the immunomagnetic beads; step 3, carrying out specific enrichment on the target bacteria; 4, performing rapid splitting decomposition to release nucleic acid; step 5, carrying out isothermal amplification on the target DNA fragment; and step 6, CRISPR / Cas12a mediated signal amplification and detection are carried out. The kit has the effects of ultra-fast detection, high sensitivity, high specificity, portability, easiness in operation, no need of bacterial culture and the like.

The invention discloses an antibody for detecting indol sulfate, a detection kit and application of the antibody, and the antibody can be specifically combined with indol sulfate and has high combination activity. The detection kit detects the content of indoxyl sulfate in a to-be-detected sample through a competitive binding reaction of an immunomagnetic bead coated indoxyl sulfate antibody, an antigen in the sample and an alkaline phosphatase labeled indoxyl sulfate antigen, and has important economic value and social significance.

The invention provides an immunomagnetic bead, a preparation method and a detection reagent. The preparation method comprises the following steps: preparing a modified magnetic bead containing a dibenzocyclooctyne group; protein and a coupling agent containing azide groups are sequentially dissolved in a second coupling buffer solution to react, modified protein containing azide groups is obtained, the final concentration of the reacted protein is 0.5-5 mg / mL, and the final concentration of the coupling agent is 0.05-5 mM; and mixing the modified magnetic beads and the modified protein according to an addition ratio of 1mg: (1-40) nmol, and reacting to obtain the immunomagnetic beads. According to the present invention, the immunomagnetic bead preparation method has advantages of high coupling efficiency, small inter-batch difference, strong specificity and difficult aggregation during the preparation process, such that the accurate detection of the antigen concentration is achieved so as to solve the defects of low coupling efficiency, large inter-batch difference and easy aggregation existing in the immunomagnetic bead preparation method in the related technology.

A magnetic bead removal method, device, and storage medium are provided. The method includes the steps of placing a liquid bag having a curved flow path on a magnetic platform, wherein a plane where the curved flow path is located is positioned above the magnetic platform and parallel to the magnetic platform; controlling a first flow rate control assembly to communicate the liquid bag with a sample container, thereby injecting a liquid containing magnetic bead-bound immune cells and non-magnetic immune cells in the sample container into the curved flow path; flattening the liquid bag to increase a contact surface between a bottom surface of the curved flow path and the magnetic platform, and adsorbing immunomagnetic bead cells in the curved flow path onto the contact surface by the magnetic platform; and performing a collection operation to collect non-magnetic immune cells in the curved flow path into a collection container.

The invention discloses an enrichment and culture method based on immunomagnetic beads, which comprises the following steps: acquiring a to-be-enriched sample, and judging the bacterial concentration of the to-be-enriched sample; if the bacterial concentration is greater than a first preset threshold value, directly performing first operation on the to-be-enriched sample; if the bacterial concentration is smaller than a second preset threshold value, performing a second operation on the to-be-enriched sample; and if the bacterial concentration is greater than a second preset threshold value and less than a first preset threshold value, performing third operation on enrichment of the to-be-enriched sample. According to the present invention, with the method, the enriched bacteria characteristics are significant, the positive reporting rate is improved, the positive reporting time is substantially shortened, the immunomagnetic beads are adopted to enrich and culture the bacteria in the sample so as to shorten the time, reduce the false negative, improve the positive reporting rate, further improve the microbiological identification level, shorten the identification time, and improve the detection accuracy. And the clinical application is better realized.

The invention relates to the field of medical detection, in particular to a mass spectrometric detection method for enriching serum human insulin by using specific antibody immunomagnetic beads. According to the present invention, the human insulin in the serum is subjected to immune enrichment by using the magnetic beads coated with the insulin specific antibody, and the concentration level of the human insulin in the serum is accurately determined based on the accurate quantification technology of the isotope dilution liquid chromatography-tandem mass spectrometry platform in combination with the bracket method quantification mode. The pretreatment method provided by the invention is simple and convenient to operate, the absolute recovery rate is excellent, the sensitivity requirement is met, and the mass spectrometry method is an isotope diluent liquid chromatography-tandem mass spectrometry method capable of eliminating interference of structural analogues.

The invention belongs to the technical field of cell culture, and particularly relates to a culture method for in-vitro amplification of NK cells activated by NK cell exosomes, which comprises the following steps of: after promoting the NK cells to generate a large amount of exosomes by using an electrical stimulation method, filtering, performing ultrafiltration, extracting and purifying the NK cell exosomes by using specific immunomagnetic beads, thereby obtaining the NK cell exosomes activated by the NK cell exosomes. A set of strict detection and quantification standards is established for the exosome by utilizing Western Blot, a BCA method, DLS and NTA technical means, a primary PBMC is obtained by adopting a density gradient centrifugation method, and the high-activity and high-purity NK cell exosome is dynamically added into a cell culture medium in the activation and amplification stages of the PBMC, so that self-activation of the NK cells is realized, the proliferation efficiency of the NK cells is remarkably improved, and the NK cell exosome has a good application prospect. Meanwhile, the safety risk of a culture system is reduced.

The invention relates to the technical field of zearalenone detection, in particular to zearalenone immunomagnetic beads as well as a preparation method and application thereof. The method specifically comprises the following steps: (1) cleaning N-hydroxysuccinimide activated agarose magnetic beads with ethanol, removing ethanol, adding a 2-morpholinoethanesulfonic acid buffer solution and a ZEN monoclonal antibody for mixed incubation, and discarding an upper solution; and (2) adding a glycine-containing buffer solution into the incubated magnetic beads, mixing, sealing, carrying out magnetic separation, and leaching to obtain the ZEN immunomagnetic beads. According to the method, IMBs high-flux full-automatic purification is combined with a high performance liquid chromatograph, the method which is rapid and convenient and can automatically treat ZEN in feed and feed raw material samples in batches is established, and the first feed industry association group standard about the IMBs purification technology is researched and formulated on the basis of the method; and a method reference is provided for prevention and control of fungaltoxin pollution in basic detection laboratories and feed enterprises.

The invention relates to the technical field of preparation of extracellular vesicles, and particularly discloses a method for preparing plant extracellular vesicles on a large scale, which comprises the following steps: (1) carrying out gradient centrifugation on a filtrate obtained by juicing and filtering plants to obtain a crude extract; (2) filtering the crude extract by using a filter membrane, removing small-particle insoluble substances in filtrate, and collecting the filtrate; (3) fully mixing the collected filtrate and the aqueous two-phase solution, performing centrifugal separation, discarding an upper phase, and collecting a lower phase; and further concentrating to obtain a plant extracellular vesicle solution. Strong denaturation chemical reagents and violent reaction are not introduced in the whole process, damage to the outer vesicles is small, an ultra-speed centrifugal machine or immunomagnetic beads are not needed, the method is suitable for industrial production, and the outer vesicles from various sources can be treated.

The invention belongs to the technical field of immunology, and particularly discloses an in-vitro preparation method and application of an induced follicular regulatory T cell iTfr.The in-vitro preparation method comprises the steps that mouse spleen single-cell suspension or healthy human peripheral blood is obtained, peripheral blood mononuclear cells are obtained, then initial CD4 + T cells are obtained through immunomagnetic bead sorting, and iTfr cells are obtained; then carrying out induced differentiation by using an antibody and a specific cytokine, and carrying out continuous stimulation and culture by adopting two different cytokine polarization conditions, so as to obtain the iTfr cell. The invention provides a method for preparing the iTfr cells in vitro, which is expected to obtain an enough number of induced follicular regulatory T cells, and provides an important tool and a treatment means for in vitro research of immune tolerance related diseases such as autoimmune diseases and organ transplantation.

The invention discloses a composite antibody immunomagnetic bead and a preparation method and application thereof, and the preparation method comprises the following steps: (1) cleaning a magnetic bead, and activating the magnetic bead with an activator; (2) adding a composite anti-erythrocyte antibody into the activated product obtained in the step (1) for antibody coupling; the composite anti-erythrocyte antibody at least comprises two anti-erythrocyte antibodies with different epitopes; (3) adding a magnetic bead sealing agent into the antibody coupling product obtained in the step (2) for sealing, and performing strong magnetic separation after the reaction is finished; and (4) adding a product obtained after strong magnetic separation in the step (3) into a freeze-drying buffer solution, uniformly dispersing, sub-packaging, adding a free red blood cell antibody, and performing vacuum freeze-drying to obtain the composite antibody immunomagnetic beads. The composite antibody immunomagnetic bead provided by the invention has excellent erythrocyte separation efficiency.

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The invention belongs to the technical field of exosome protein detection, and provides an SERS (Surface Enhanced Raman Scattering) sensing platform for synchronously and jointly detecting exosome protein and a preparation method of the SERS sensing platform. Preparing a magnetic bead-exosome compound, and respectively marking the three proteins on the surface of the exosome by using different SERS tags; depositing silver nanoparticles in situ on the immunomagnetic bead-exosome compound; preparing a gold film array substrate for SERS (Surface Enhanced Raman Scattering) detection, and obtaining SERS signals corresponding to each protein on the exosome by utilizing an SERS synergistic enhancement effect of multistage signal amplification. A SERS sensing platform integrating separation, enrichment and detection is constructed and is used for high-sensitivity detection of exosome protein. The binding efficiency of the exosome and the precious metal Raman probe is remarkably improved, and the feasibility of synchronous combined detection of multiple proteins in the same sample is improved. The method is high in detection sensitivity and good in specificity, and high-throughput detection of exosome protein can be realized.

The invention discloses a biological chip for simultaneously detecting multiple abundance markers and application thereof, and belongs to the technical field of immunodetection. The biochip comprises a PDMS layer, a glass substrate layer, a detection channel formed between the PDMS layer and the glass substrate layer, and a chip inlet and a chip outlet which are communicated with the detection channel, the detection channel comprises a CA19-9 detection channel, a CA125 detection channel and an LIF detection channel; the CA19-9 detection channel and the CA125 detection channel are filled with capture antibody modified immunomagnetic beads and immunofluorescence modified detection antibodies; the LIF detection channel is filled with an immunofluorescence modified detection antibody, and the surface of the glass substrate layer in the LIF detection channel is modified with an Anti-LIF capture antibody. The invention discloses a biochip for simultaneously detecting multiple abundance markers with high capture concentration and application of the biochip.

The invention discloses a method for extracting tumor and infectious disease body fluid free nucleic acid, a kit and application. The extraction method provided by the invention is high in extraction efficiency; through comparison, the extraction quality is 10 times that of a traditional kit. The kit comprises a specific MOF adsorption reagent, a lysis solution, a separating agent and a nucleic acid storage tube, the specific MOF adsorption reagent can capture free nucleic acid in body fluid of a patient through specific immunomagnetic beads, and then the free nucleic acid is separated and extracted through the lysis solution and the separating agent. According to the kit provided by the invention, free nucleic acid in tumor or infectious disease body fluid can be specifically extracted, interference of other normal cell DNA is eliminated, and the kit can be used for scientific research or clinical diagnosis analysis such as PCR amplification, gene expression, gene sequencing, chip detection, library construction and high-throughput sequencing, so that the subsequent detection result is more accurate, and the detection efficiency is improved. And the detection effect is better.

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

The invention discloses a plasma or serum exosome separation method which comprises the following steps: firstly, coupling an antibody for resisting four transmembrane protein CD81 on the surface of an exosome by using a magnetic bead to form an immunomagnetic bead; pretreating a serum / plasma sample, enriching exosomes, co-incubating with the immunomagnetic beads, washing the magnetic beads after incubation capture is completed, and washing off uncombined impurities; after being captured, the exosome can be directly cracked, the magnetic beads are removed for downstream experiments, or the magnetic beads are eluted to obtain the complete exosome. The exosome affinity antibody is matched with the magnetic beads, the plasma / serum sample exosome is separated, the unique affinity separation technology not only can remove high-abundance protein in the serum / plasma sample, but also can eliminate other vesicles with similar membrane structures with the exosome, so that the purity of the separated exosome is far higher than that of the exosome obtained by other separation methods.

The present invention relates to the technical field of the detection of ancient silk fabrics, and discloses a preparation method and application of an immunomagnetic bead test strip for rapidly detecting trace proteins in cultural relics, comprising the following steps: (1) after the carboxyl magnetic beads are subjected to a coupling reaction with protein antibodies, the supernatant is removed by magnetic separation, and after washing with a buffer solution and a cleaning agent in sequence, immunomagnetic beads are obtained; (2) the immunomagnetic beads are sprayed on a pretreated nitrocellulose membrane, and after drying, they are reserved for use; the diluted antibody solution I and antibody solution II are respectively sprayed on the test line and the quality control line of the nitrocellulose membrane, and after drying, they are reserved for use; (3) a sample pad, an immunomagnetic bead conjugate pad, a nitrocellulose membrane and a water absorption pad are assembled and cut in sequence to obtain an immunomagnetic bead test strip, which is stored in a dry and sealed manner. The present invention can achieve rapid and sensitive detection while enriching trace proteins, is convenient for operation at the archaeological site, and takes a short time.

The present application relates to the technical field of biotechnology, and particularly relates to a D-dimer chemiluminescence assay kit. The present application provides a D-dimer chemiluminescence assay kit, which comprises a magnetic particle suspension (immunomagnetic beads) coated with D-dimer antibody 1, alkaline phosphatase-labeled D-dimer antibody 2 (enzyme-labeled antibody) and a calibrant. The immunomagnetic beads and the enzyme-labeled antibody are prepared by chemical coupling and stored in a buffer containing a soluble high molecular material, which can effectively improve the detection sensitivity and repeatability. The kit can be used in combination with a substrate solution containing a chromogenic substrate such as adamantane and its derivative AMPPD or APS-5 for a full-automatic immune test system, and the content of D-dimer in plasma or whole blood can be determined by a chemiluminescence instrument. The kit has high detection sensitivity and good repeatability.

This invention discloses a microfluidic product plate for screening tumor cells using immunomagnetic beads, belonging to the field of tumor cell screening technology. It includes a dust-free injection-molded or micro-injection-molded product core plate with a pipeline processing mechanism mounted on it for screening target cells in injected liquid. The pipeline processing mechanism includes an input pipe, several delivery pipes, a first connecting pipe, an electromagnetic adsorption unit, a second connecting pipe, and an output pipe. The input pipe is connected to a sample injection pipe, a sheath flow injection pipe, an immunomagnetic bead injection pipe, and a blood cell recovery pipe. The output pipe is connected to a second sheath flow injection pipe, a waste liquid recovery pipe, and a target cell recovery pipe. The first sheath flow injection pipe, the immunomagnetic bead injection pipe, the blood cell recovery pipe, the electromagnet, and the second sheath flow injection pipe work together to separate impurity cells at the front end of the same product core plate, inject and mix immunomagnetic beads in the middle, and precisely sort target cells at the rear end.

The invention discloses a giardia immunomagnetic bead-immunofluorescence detection kit based on beta-giardin and a preparation method of the giardia immunomagnetic bead-immunofluorescence detection kit, and provides a newly identified giardia specific cyst outer wall protein antigen beta-giardin. The immunomagnetic bead-immunofluorescence detection method prepared on the basis of beta-giardin protein of giardia has the advantages of high specificity, high sensitivity, good stability, simplicity and convenience in operation, diversity of detection samples, low false detection rate and the like. The detection result is suitable for rapid diagnosis and detection in a clinical field, and the detection method can reduce the risk of occurrence and transmission of giardia disease to a great extent.

The invention relates to the technical field of micro-fluidic detection equipment, and provides an immunomagnetic bead detection system based on a micro-fluidic chip, the immunomagnetic bead detection system comprises a support and the micro-fluidic chip, and a magnetic field device comprises a magnet; the device is characterized in that the magnet is a cylinder magnetized in the diameter direction, the central axis of the magnet is perpendicular to the bottom face of the reaction bin, the diameter of the magnet is larger than the width of the reaction bin, the diameter of the magnet is smaller than the length of the reaction bin, and the diameter of the magnet is larger than or equal to two times of the width of the reaction bin. The magnetic field intensity of the surface of the magnet ranges from 20 mT to 150 mT; meanwhile, the invention also provides a detection method applying the immunomagnetic bead detection system based on the micro-fluidic chip. The magnetic bead detection system has the beneficial effects that the magnetic beads left in the reaction bin after a user washes the magnetic beads are few, the cleaning effect is good, and the detection limit is reduced; the detection method has the advantages of low detection limit and high detection speed.

The invention discloses an immunomagnetic bead with thermomagnetic dual response, a preparation method of the immunomagnetic bead and application of the immunomagnetic bead in detection of food-borne pathogenic bacteria. The preparation method comprises the following steps: (1) preparation of the magnetic core; (2) construction of the inorganic shell layer; (3) introduction of the temperature-sensitive polymer layer; (4) surface functionalization and streptavidin coupling; organic combination of the thermal response polymer and the magnetic core-shell structure is realized for the first time, so that the immunomagnetic bead has dual response capabilities of temperature control gathering and scattering and magnetic field separation. Under a low-temperature condition, the magnetic beads are kept in a dispersed state, so that target combination is facilitated; after the temperature is raised, the polymer is subjected to hydrophobic collapse, particles are gathered, and rapid capture is realized through a magnetic field, so that the detection efficiency and the binding stability are greatly improved.

The invention provides an anti-RBD immunomagnetic bead as well as a preparation method and application thereof, and belongs to the technical field of immunodiagnosis. The preparation method of the anti-RBD immunomagnetic beads comprises the following steps: washing aminated magnetic beads with a 1 * PBST solution, and removing supernatant after washing; adding 15% glutaraldehyde, activating in a dark place at room temperature, removing the supernatant after activation, adding 1 * PBST solution to wash the magnetic beads, and removing the supernatant after washing; adding a 28D9 antibody, incubating at room temperature in a dark place, and removing supernatant after incubation is finished; adding a 1 * PBST solution containing 5% of BSA (Bovine Serum Albumin) for sealing at room temperature in a dark place, removing supernatant after sealing is finished, washing the magnetic beads by using the 1 * PBST solution, and removing supernatant after washing is finished; and adding a preserving fluid to obtain the anti-RBD immunomagnetic bead. The anti-RBD immunomagnetic bead provided by the invention can be used for rapidly purifying RBD protein, so that the time is greatly saved for the production of new crown protein vaccines.

The present application relates to a kind of human gastric cancer primary fibroblast separation culture and purification method and its application.The human gastric cancer primary fibroblast separation culture and purification method provided by the present application are simple, efficient.The separation culture method and the purification method provided by the present application can complete tissue dissociation in shorter time, and cooperate multiple immunomagnetic beads to remove miscellaneous cells, so that the gastric cancer primary fibroblast group of stable, relatively consistent morphology adherent growth is obtained, phenotype observation is facilitated, and the interference of miscellaneous cells to subsequent function experiment is reduced.Enriched cells are expanded after culture, and can be used for the subtype research of fibroblast, microtumor environment related function experiment.

The invention relates to the field of CAR-T cell concentration detection, in particular to a method for detecting CAR-T cells through combination of microfluidics and immunofluorescence. Through immunomagnetic bead enrichment and micro-fluidic chip partitioning, CAR-T cells are separated from a blood sample through immunomagnetic beads, then labeling is carried out or enhanced by adding a labeling antigen, anti-TCR-PE and DAPI, three-channel fluorescence labeling is achieved, quantitative analysis can be carried out on the number, the cell morphology and the function of the CAR-T cells through automatic immunofluorescence imaging, and the accuracy of CAR-T cell detection is improved. And the detection limit reaches 0.001%.