623 results about "Immunomagnetic bead" patented technology

The invention relates to a vibrio parahemolyticus detection kit. The vibrio parahemolyticus detection kit is characterized by comprising the following components: (1) an immune enrichment reaction system component, (2) a loop-mediated isothermal amplification (LAMP) system component and (3) a set of specific primers based on an LAMP technology of vibrio parahemolyticus tlh genes, wherein the specific primers comprise two outer primers F3 and B3, two inner primers FIP and BIP and two ring primers LF and LB. The detection method for the vibrio parahemolyticus detection kit comprises the following steps of: detecting the vibrio parahemolyticus by adopting a method of combining immune enrichment and the LAMP technology; preparing an immunomagnetic bead by adopting a vibrio parahemolyticus polyclonal antibody; preliminarily screening the vibrio parahemolyticus in an actual sample by adopting an immunology method; and designing an LAMP specific primer according to the species specificity gene of the vibrio parahemolyticus. According to the invention, molecular detection is carried out on a nucleic acid level, therefore, the condition of false positive or undetection of a simple immunology method or a molecular method is effectively avoided, and the invention is a new development direction of the quick detection of the vibrio parahemolyticus.

An immune bead is made up of the magnetic carrier micro ball which combines at least one immune matching base. The micro ball is composed of the magnetic nm particle and the high molecular framework material which the core is the metal particle; the out of the core is high molecular framework, the out layer is the functional layer which can combines functional gene of different immune matching base. The manufacture method includes: the bead pretreatment, the bead activation, manufacture of the coupling antibody, closing the antibody with the confining liquid and purifying the immune bead. The detecting method is to detect the different things by the immunological response sandwich, the competition and the indirect method and set the control system on the testing board. The board is made up of the encrusting test paper, the coupling mat, the sample mat, the water suction mat, the coving film and the testing board outside calipers. It has the high sensitivity and accurate quantity; the regent is simple and cost low.

Disclosed is a disk based platform for separating and detecting cells that are labeled with immunomagnetic beads. A disk-like carrier board forms therein at least one flow channel structure, which includes an inner reservoir for receiving a sample fluid, an outer reservoir, and a plurality of micro flow channels arranged between and in fluid communication with the inner and outer reservoirs. When the disk-like carrier board spins, cells labeled with immunomagnetic beads are attracted by a magnetic attraction unit that is arranged above the disk-like carrier board and adjacent to the inner reservoir to thereby remain in the inner reservoir, and cells that are not so labeled are acted upon by a centrifugal force induced by the spinning of the disk-like carrier board to flow with the sample fluid from the inner reservoir through the micro flow channels into the outer reservoir.

The invention provides a construction method of a cell model for detecting pyrogens, the cell model and a pyrogen detection kit. The cell model utilizes specific locations of CRISPR / CAS9 induced genomes to form double-bond fission, TLR4 and CD14-MD2 are knocked into two chromosomes of a cell line respectively by the aid of the homologous recombination repair principle, green fluorescence GFP and red fluorescence RFP are respectively used for tracing finally successfully constructed TLR4 / CD14 / MD2 fixed-point knocked-in fluorescent tracer cell models, and the LPS stimulating cell model can detect release of IL-6 and TNF-a cytokines by means of ELISA, Western Blot, mass spectrum and immunomagnetic beads. The cell model is good in stability and high in sensitivity, and the lowest detectable limit can reach 0.005EU / mL and is far lower than 0.025EU / mL of the Tachypleus Amebocyte Lysate method.

The invention relates to a medical testing kit for performing quantitative detection on human serum RBP4 using chemiluminescence magnetic-enzyme immunotherapy. The kit is composed of four reagent parts: specificity mouse anti-human RBP4 custodite immunomagnetic beads, enzyme labelling specificity mouse anti-human RBP4 antibody II, chemiluminescence substrate, corresponding titer and quality control liquid. The using method of the kit comprises: using bead particulates as solid phase carrier, combining specificity mouse anti-human RBP4 antibody I on the surface, forming RBP4 specificity immunocompetence beads, capturing antigen RBP4 to be detected in the enzyme labelling specificity mouse anti-human RBP4 antibody II, forming double antibody sandwich composite on the surface of the beads, wherein enzyme marked on the composite reacts with corresponding irradiance substrate in the reaction system to form stable luminous signals, thereby reaching quantitative detection and analysis on RBP4 through strength of the detection light signals. The invention has the advantages of high sensitivity, high specificity, simple and fast operation.

The invention provides a circulating tumor stem cell detection kit based on magnetic beads and a microfluidic chip. The circulating tumor stem cell detection kit is characterized by comprising a microfluidic chip, at least one immunomagnetic bead, and at least one fluorescent antibody against a tumor stem cell biomarker, wherein the immunomagnetic bead can be specifically bonded with the circulating tumor stem cell and marked with the tumor stem cell biomarker; the microfluidic chip comprises a glass chip base; a microfluidic channel is arranged on the glass chip base; a soft magnetic micro-array is arranged inside the microfluidic channel. By adopting the circulating tumor stem cell detection kit, a plurality of different categories of rare circulating tumor stem cells can be captured and detected by sampling once, and the circulating tumor stem cell detection kit has high sensitivity and specificity, is convenient and fast to operate, can easily collect captured cells, and does not need complicated surface modification processes of a first antibody and a second antibody of the microfluidic channel inside the chip.

It is a detecting ischemia modified albumin (IMA) reagent kit and detection method, and the reagent kit contains different division to remove the background interference material device, which can be centrifugal ultrafiltration IMA detection reagent kit, immunomagnetic beads IMA detection reagent kit, immunochromatography IMA detection reagent kit, immune chromatography membrane IMA detection board reagent kit or immune membrane filtration plate IMA detection reagent kit. After division removal of the background interference material, it can enhance the specific of detection IMA reagent kit and detection method, and using this reagent kit and hospital existing equipments, in about half an hour, it can measure the IMA value to diagnosis myocardial ischemia symptoms. Particularly suitable for bedside rapid detection kit development, ease to use, low cost, it is a very development promising product.

The invention discloses an efficient multiplication CTL preparation method killing tumors in a targeted mode. The CTL preparation method comprises the following steps: (a) removing CD4+CD25+Treg cells through immunomagnetic bead negative sorting; (b) arranging mixed cells in a serum-free medium for cultivation, and obtaining suspension cells and adherent cells; (c) adding GM-SCF and IL-4 in the adherent cells, culturing the cells for five days; in the sixth day, adding a tumour cell holoantigen, and in the seventh day, adding TNF-alpha and IL-27; (d) transferring the suspension cells to a culture flask wrapped by a CD3 monoclonal antibody and recombinant human fibronectin, adding IFN-gamma, in the second day, adding IL-2, IL-12 and the IL-27, and culturing the mixture till the eighth day to obtain CIK cells; (e) mixing the CIK cells and mature DC cells, and adding the IL-12, IL-7 and an anti-CD 28 monoclonal antibody for cultivation; in the third day, adding an anti-CTLA-4 monoclonal antibody, and then culturing the mixture for four days. According to the efficient multiplication CTL preparation method killing tumors in the targeted mode, efficiency of in-vitro CTL cell proliferation is improved, activity of killing the tumor cells in the targeted mode is improved, transformation of peripheral blood mononuclear cells to the CD4+CD25+Treg cells is inhibited.

Disclosed is a disk based system for separating at least two types of particulates contained in a sample fluid. The system includes a disk-like carrier board and a magnetic attraction unit. The disk-like carrier board forms at least one flow channel structure, which includes an inner reservoir, at least one separation chamber, and at least one outer reservoir arranged in sequence from a geometric center of the disk-like carrier board to an outer circumferential rim of the disk-like carrier board. A method of separation carried out with the system includes introducing the sample fluid into the inner reservoir and then rotating the disk-like carrier board to induce a centrifugal force. The sample fluid contains particulates that are labeled with immunomagnetic beads and the labeled particulates are attracted by the magnetic force generated by the magnetic attraction unit to retain in the inner reservoir or the separation chamber. Particulates not labeled with the immunomagnetic beads and contained in the sample fluid move with the sample fluid to the outer reservoir.

The invention discloses a quantitative creatine kinase-myoglobin (CK-MB) determination kit. The kit is characterized in that the kit contains CK-MB magnetic separation reagent, enzymatic reactant, reaction enhancer, diluent, CK-MB calibrator, CK-MB quality control material, cleaner concentrate and substrate solution. The invention also discloses a preparation method for the kit. The kit integrates the chemiluminescence technology with immunomagnetic beads to provide a homogeneous phase-approximating reaction system. Compared with the prior art, the kit has higher assay sensitivity and specificity, and achieves better performance parameters, and mover, the product cost is greatly reduced.

The invention discloses a method for separating exosomes from serum by using immunomagnetic beads. The method comprises the following steps: pretreating the serum, coupling a monoclonal antibody with magnetic beads, and separating the exosomes from the serum by using an antibody-magnetic bead compound. The method has the advantages as follows: the method is easy to operate and low in cost; by the method, the exosomes containing specific markers can be separated from the serum.

The invention discloses a CEA TRFIA (time-resolved fluoroimmunoassay) kit based on IMB (immunomagnetic beads). The kit includes a calibrator, immunomagnetic beads connected with CEA antibodies, europium labeled CEA antibodies, an assay buffer solution, a cleaning solution and an enhanced solution. Through double antibody sandwich immunoreaction, IMB-CEA-europium labeled-CEA monoclonal antibody complexes are formed, IMB which adsorb CEA and supernate are separated through magnetic seperation and washed, the enhanced solution is added, and the value is measured through a time-resolved instrument. Besides the TRFIA technology, the invention further has the advantages as follows: through IMB enrichment and full diffusion of IMB in the solution, the combination superficial area is enlarged, the reaction time is greatly shortened, and the detection sensitivity is improved. IMB and antibodies are directionally connected through chemical groups, so that the consumption of paired antibodies is greatly reduced and the detection precision is improved. The technology realizes automation easily, and overcomes the problem that the traditional micropore plate type TRFIA technology can only carry out detection after samples are accumulated to a certain number, thereby realizing real-time sample detection.

The invention relates to an isolated culturing method for tumor-specific TIL cells. According to the method, one or more from PD-1, LAC-3 and TIM 3 are utilized for serving as markers for separating tumor specific T-lymphocytes in TIL cells, the immunomagnetic bead technology is combined, the tumor specific T-lymphocytes are separated from a TIL cell mass, then in-vitro efficient amplification is conducted, and the tumor-specific TIL cells with high tumor killing activity are obtained. By means of the isolated culturing method for the tumor-specific TIL cells, the separated culturing operation procedure of the tumor-specific TIL cells is made to be simpler, the separated culturing time is obviously shortened, the cost is low, the application and popularization are facilitated, and moreover through the separation, the obtained tumor-specific TIL cells are higher in tumor cell killing activity.

The invention provides a full-automatic luminescent immunoassay system based on a micronano-magnetic bead electromagnetic transfer technique. The system includes a computer and a detection box. The detection box is a sealable lightproof box, and comprises a first motor, a second motor, a membrane rupture device, an electromagnetic stirring rod, a Teflon coating or a disposable plastic jacket, immunomagnetic beads, a sample pool, a temperature control device, a photoelectric conversion device, a bottom reading light path, a waste consumable material recovery area or ultrasonic cleaning equipment, a reagent bin consumable material and a consumable material adapter, a guide rail and an electromagnet. The system provided by the invention is based on a magnetic bead transfer method, simplifies the detection steps, integrates the detection module, avoids a complex liquid path system, saves the detection time while realizing full automation and miniaturization of the immunodetection system, and also lowers the system cost at the same time.

The invention discloses an in-vitro large-scale amplification method of natural killer cells. The method comprises the following steps: collecting and separating peripheral blood mononuclear cells; sorting natural killer cells; culturing the natural killer cells; and collecting the natural killer cells. The method utilizing immunomagnetic beads to sort the natural killer cells has the advantages of simplicity in establishing and operation, and high comprehensive efficiency; and the effect of the method adopting in-vitro amplification of the immunomagnetic bead shorted NK cells is 2-3 times the effect of present amplification methods, so the method disclosed in the invention has great application values in the field of in-vitro large-scale amplification.

The invention discloses a gastrin-17 enzymatic chemiluminescence immunoassay kit and belongs to the technical field of chemiluminescence immunoassay analysis. The kit comprises an enzyme label liquid, a gastrin-17 standard, gastrin-17 monoclonal antibody-coated immunomagnetic beads, a sample diluent, a chemiluminescent substrate liquid and a washing liquid. The principle of the gastrin-17 enzymatic chemiluminescence immunoassay kit comprises that a gastrin-17 monoclonal antibody is connected to the surface of a magnetic bead so that a solid phase agent is obtained, and through capture of gastrin-17 in a sample and use of an enzyme-labeled anti-gastrin-17 monoclonal antibody, a solid phase-antibody-antigen-enzyme-labeled antibody sandwiched immune complex is formed. Through combination of a chemiluminescence technology and an immunomagnetic bead technology, the prepared kit has the advantages of high sensitivity, good specificity, wide linearity range and good stability and can satisfy clinical requirements on stomach function detection.

The invention provides a method for quantitative determination of an associated antigen by means of immunomagnetic beads. The method comprises the steps of coating an antibody with the beads in a coupling mode to obtain a bead solution of the coupled and coated antibody, mixing the bead solution of the coupled and coated antibody with a sample containing the associated antigen so that the antigen can be combined with the antibody coupled to the beads through specific immunity reaction, then adding an antibody solution marked with fluorescent microspheres so that specific immunity reaction can be conducted on the antibody marked with the fluorescent microspheres and the antigen on the beads, and detecting a fluorescence signal to obtain the content of the antigen. The detection method has the advantages that sensitivity is high, specificity is high, the problem that a certain kind of antibody is not coated firmly in certain occasions can be solved, quick detection of antigens can be achieved, stability is high, and detection results are accurate.

The invention discloses an immunomagnetic beads preservation solution, which comprises a buffer, an inert protein, glycerin, a water-soluble sugar, a synthetic high molecular polymer, a protein stabilizer, a reducing substance, an amino acid, a chelating agent, a serum, a surfactant, and a preservative. According to the immunomagnetic beads preservation solution of the invention, the activity of immunomagnetic beads can be maintained at a high level within one year at 4 DEG C storage conditions, thereby providing a guarantee for the practical detection of the immunomagnetic beads.

The invention relates to the technical field of magnetic separation on biological samples. The invention discloses an IMB (immunomagnetic bead) based sequential fluid type fully-automatic magnetic separation device, which comprises a control system and a fluid-channel system, wherein the control system comprises a master control module, a precise injection pump, an electromagnetic three-way valve, an electrically operated multi-position valve and an electromagnet; the fluid-channel system comprises a sample feeding column, a magnetic bead column, a mixed column, a magnetic separation column, a sample discharge column, a cleanout fluid bottle, a spent liquor bottle, an interim tube and a pipeline communicated with the above components; the master control module is used for controlling the precise injection pump, the electromagnetic three-way valve, the electrically operated multi-position valve and the electromagnet to execute corresponding actions according to a preset process and a preset time sequence, so that the samples to be separated and related reagents are transferred and processed in the fluid-channel system according to a certain sequence. The invention also discloses a method for carrying out automatic magnetic separation by using the above device. The device and the method of the invention can satisfy the requirements for field or unattended automatic magnetic separation.

The invention relates to a method for detecting fumonisins based on immuno-magnetic bead combined enzyme-linked immunosorbent assay, belonging to the technical field of chemical detection. The method comprises the steps of: preparing fumonisins-KLH conjugate and fumonisins-OVA conjugate, combining the fumonisins-KLH conjugate with an immuno-magnetic bead, and preparing a magnetic bead for detecting the fumonisins; then, using the fumonisins-KLH conjugate to prepare fumonisins monoclonal antibody, applying a competition ELISA method for detecting the fumonisins together with the magnetic bead for detecting the fumonisins, and obtaining the magnetic bead for detecting the fumonisins by a magnetic separating method; and developing and obtaining the detection result by an enzyme linked immunosorbent assay. The method is used for the fumonisins sample which is lower than detection limit, and enlarges the combination superficial area by enrichment of the immuno-magnetic bead and the full diffusion of the magnetic bead in the liquid, thus indirectly changing the detection limit, improving the detection sensitivity and avoiding undetected error.

The invention adopts double-function gold magnetic nanoparticles and provides a detection method which integrates an immunomagnetic bead capturing technology and an immunochromatography technology and is used for rapidly detecting salmonellas. Immunomagnetic separation and immunochromatography are organically integrated, the step of eluting the salmonellas from immunomagnetic beads is eliminated and the capture efficiency is improved; the step of spraying colloidal gold on a bonding mat is eliminated, the immunological reaction is more uniform and a variable coefficient is small in the quantitative detection; workload and probability of mixed bacterium pollution are reduced. The detection method adopts the basic thinking of exploring the mode of effectively combining the gold magnetic nanoparticles and an antibody, optimizing the conditions of enriching the salmonellas and carrying out rapid quantitative detection by using the immunochromatography technology as a vector and using the double antibodies sandwich as a detection principle.

The invention relates to a relaxation time immunosensing analysis method based on magnetic separation. The method comprises the following steps: selecting two magnetic beads (one can be quickly separated and the other cannot be separated) different in saturated magnetization intensity and remarkably different in separation speed in the same magnetic field, so that the magnetic beads can be separated in the magnetic field; coupling the magnetic beads to an antibody used for identifying different sites of the same target object respectively to prepare immunomagnetic beads; producing an immunoreaction of the immunomagnetic beads and a to-be-detected sample; performing magnetic separation on a mixed system, measuring transverse relaxation time for supernatant liquor subjected to magnetic separation, and determining the concentration of biomacromolecules in the to-be-detected sample according to change of the transverse relaxation time. The immunomagnetic beads different in saturated magnetization intensity are different in separation speed in the same magnetic field, the magnetic separation is combined with magnetic relaxation time analysis, the reaction time only needs 30 minutes, and the method can be used for quickly detecting bacteria, viruses and proteins and has a very good application prospect in an aspect of biomarker detection.

The invention provides an immune agglomeration detection method based on a micro-fluidic chip. The immune agglomeration detection method comprises the following steps: dynamically enriching immunomagnetic beads by virtue of a micro-fluidic chip by controlling a specific region of a magnetic field in a chip micro-channel to form a magnetic bead plug; controlling a sample liquid to flow through the magnetic bead plug in cycle and capturing antigen in the sample liquid by the immunomagnetic beads; combining the antigen captured by the immunomagnetic beads with another magnetic beads to form two or more agglomerations of the magnetic beads; and counting the quantity of single magnetic beads and the agglomerated magnetic beads by detecting scattered light signals of the magnetic beads to obtain the concentration of the antigen in the sample. The invention provides the micro-fluidic chip. The invention further provides an immune agglomeration detection system based on the micro-fluidic chip. The detection system comprises the micro-fluidic chip, a micro-pump drive device, a micro-valve drive device, a magnetic bead plug control device and an optical detection module. According to the immune agglomeration detection method and system, achievement of a miniature immune detection instrument is facilitated; the detection limit is reduced; and detection of low-concentration antigen is achieved.

The invention discloses an efficient whole-genome chromosome conformation capture technology (eHi-C). The invention provides an eHi-C sequencing technology for cells. The eHi-C sequencing technology comprises the following steps: 1) subjecting the cells to disintegration so as to obtain chromatin; 2) subjecting the chromatin to enzyme digestion, DNA end labeling, cyclization connection of a blunt end and DNA purification so as to obtain purified circular DNA; 3) introducing a circular co-precipitation DNA molecule used as internal reference; 4) carrying out ultrasonic breaking; 5) capturing all the labeled DNA fragments with immunomagnetic beads; and 6) preparing eHi-C sequencing library from the labeled DNA fragments. The technology provided by invention is low in the amount of needed cells, can easily acquire an experiment material, reduces time in acquisition of the material, greatly lowers cell culture cost and cost for reagent consumables and decreases test errors.

The invention discloses a kit for quantitatively testing free triiodothyronine, which comprises 3,3'-L-Diiodothyronine-gelatin enveloped magnetic bead suspension, a free triiodothyronine series calibrator, a horse radish peroxidase labeled free triiodothyronine antibody, chemiluminescent substrate A solution, chemiluminescent substrate B solution, and PBS buffer solution. The invention also discloses a method for preparing the kit. The kit has the advantages that: the chemiluminescence technology is combined with the immunomagnetic bead technology, the immunomagnetic beads are taken as a reaction carrier, the effective envelop amount of antigen is increased, raw materials are saved and the detection sensitivity and detection speed are obviously improved. A method for enveloping antigen analogs by the labeled antibody is adopted, so the analogs can be specifically combined with the antibody of hormone, but the combination capacity with thyroxine-binding protein is greatly reduced, and the influence of the binding protein on a measurement system is reduced to a large extent.

The invention discloses a detection kit and a detection method of circulating tumor cells. The kit comprises a PBS buffer solution, a density gradient separation medium, a red blood cell lysate, a CD45 immunomagnetic bead, an incubation solution, a fixing solution, a permeabilizing solution, a confining liquid, an antifade mounting medium, an antibody dilution solution and a fluorescent antibody concentrate, and the fluorescent antibody concentrate contains a fluorescent dye pan-CK-AF488, a fluorescent dye CD45-PE and a cell nucleus dye Hoechst33342. The detection method comprises the following steps: S10, centrifuging a blood sample, and separating and enriching the CTCs; and S20, carrying out dyeing identification on the enriched CTCs through the cell nucleus dye pan-CK-AF488, the fluorescent dye CD45-PE and Hoechst33342 fluorescence antibodies, wherein the detection is carried out for the purpose of non-treatment. When the detection kit and the detection method are adopted to perform detection, the detection result is accurate and reliable.

A placenta original filled stem cell and its production are disclosed. The process is carried out by bleeding after delivering placenta from uterus, perfusing to obtain single-cell soliquoid from continuous perfusion method or collagenase digestion method and acquiring filled stem cell from gradient and density centrifugation or immune magnetic bead separation. It has better external enrichment potentiality and multidirectional differential potentiality and low immunogenicity.

The invention relates to a separation and efficient amplification culture method for an antigen specific T lymphocyte. By means of the immunomagnetic bead technology and the character of an active T cell expression CD137, the antigen specific T lymphocyte is separated from a complex cell mass; then an antigen specific T lymphocyte with high tumor killing activity is obtained through in-vitro efficient amplification culture. CD137L (CD137-ligand), IL-7, IL-15 and IL-2 are added in the culturing process, so that the phenomenon that activated and induced cells are liable to apoptosis in the culturing process is avoided, the amplification time is increased, and the killing activity of the antigen specific T lymphocyte is improved.

The invention applies difunctional nanogold particles and provides a listeria monocytogenes rapid detection method integrating an immunomagnetic bead capture technique and an immunochromatography technique. The immune magnetic separation and immunochromatography are organically integrated, the step of eluting the listeria monocytogenes from an immunomagnetic bead is eliminated, so the capture efficiency is improved; the step of spraying colloidal gold on a binding pad is eliminated, so that the immunological reaction is much evener, and the variable coefficient is small during quantitative determination; and the workload and the living contaminant rate are reduced. The basic train of thought of the detection method is exploring the effective combination method of the nanogold magnetic particles and an antibody, optimizing the condition of enriching the listeria monocytogenes on the nanogold magnetic particles and the antibody, and conducting rapid quantitative detection by using the immunochromatography technique as a carrier and a double-antibody sandwich as a detection principle.

The invention discloses a microfluidic chip detection method based on magnetic bead technology. The method includes the steps of: 1, fluorescence labeling; 2, magnetic bead labeling; 3, coating and drying of a blocking solution; 4, immobilization of immunomagnetic beads; 5, immobilization of a fluorescence label; 6, microfluidic chip assembly; 7, sample adding preparation; 8, immunoreaction; 9, cleaning; 10, color development; and 11, data reading. The method provided by the invention selects highly sensitive time resolved fluorescent dye as the label for antibody labeling on magnetic beads and a fluorescent substance respectively, and utilizes the immunoreaction of the antibody pair for analysis and detection, and the performance of the prepared reagent can reach the level of equivalent chemiluminescent reagents. At the same time, the reagents adopt homogeneous liquid reaction to realize control of liquid flow. Ultrasonic mixing is adopted in the microfluidic chip chamber to avoid single-threaded chromatography flow reaction, therefore the reaction is fuller, and the reaction efficiency is higher.