135 results about "Immunomagnetic bead" patented technology

The invention provides a method for detecting salmonella enteritidis, which comprises the following steps of: 1, expressing a nano antibody for resisting salmonella enteritidis FliC by using escherichia coli, and verifying the nano antibody; step 2, preparing immunomagnetic beads for resisting FliC protein, and verifying the immunomagnetic beads; step 3, carrying out specific enrichment on the target bacteria; 4, performing rapid splitting decomposition to release nucleic acid; step 5, carrying out isothermal amplification on the target DNA fragment; and step 6, CRISPR / Cas12a mediated signal amplification and detection are carried out. The kit has the effects of ultra-fast detection, high sensitivity, high specificity, portability, easiness in operation, no need of bacterial culture and the like.

The invention discloses an antibody for detecting indol sulfate, a detection kit and application of the antibody, and the antibody can be specifically combined with indol sulfate and has high combination activity. The detection kit detects the content of indoxyl sulfate in a to-be-detected sample through a competitive binding reaction of an immunomagnetic bead coated indoxyl sulfate antibody, an antigen in the sample and an alkaline phosphatase labeled indoxyl sulfate antigen, and has important economic value and social significance.

The invention provides an immunomagnetic bead, a preparation method and a detection reagent. The preparation method comprises the following steps: preparing a modified magnetic bead containing a dibenzocyclooctyne group; protein and a coupling agent containing azide groups are sequentially dissolved in a second coupling buffer solution to react, modified protein containing azide groups is obtained, the final concentration of the reacted protein is 0.5-5 mg / mL, and the final concentration of the coupling agent is 0.05-5 mM; and mixing the modified magnetic beads and the modified protein according to an addition ratio of 1mg: (1-40) nmol, and reacting to obtain the immunomagnetic beads. According to the present invention, the immunomagnetic bead preparation method has advantages of high coupling efficiency, small inter-batch difference, strong specificity and difficult aggregation during the preparation process, such that the accurate detection of the antigen concentration is achieved so as to solve the defects of low coupling efficiency, large inter-batch difference and easy aggregation existing in the immunomagnetic bead preparation method in the related technology.

A magnetic bead removal method, device, and storage medium are provided. The method includes the steps of placing a liquid bag having a curved flow path on a magnetic platform, wherein a plane where the curved flow path is located is positioned above the magnetic platform and parallel to the magnetic platform; controlling a first flow rate control assembly to communicate the liquid bag with a sample container, thereby injecting a liquid containing magnetic bead-bound immune cells and non-magnetic immune cells in the sample container into the curved flow path; flattening the liquid bag to increase a contact surface between a bottom surface of the curved flow path and the magnetic platform, and adsorbing immunomagnetic bead cells in the curved flow path onto the contact surface by the magnetic platform; and performing a collection operation to collect non-magnetic immune cells in the curved flow path into a collection container.

The invention belongs to the technical field of immunology, and particularly discloses an in-vitro preparation method and application of an induced follicular regulatory T cell iTfr.The in-vitro preparation method comprises the steps that mouse spleen single-cell suspension or healthy human peripheral blood is obtained, peripheral blood mononuclear cells are obtained, then initial CD4 + T cells are obtained through immunomagnetic bead sorting, and iTfr cells are obtained; then carrying out induced differentiation by using an antibody and a specific cytokine, and carrying out continuous stimulation and culture by adopting two different cytokine polarization conditions, so as to obtain the iTfr cell. The invention provides a method for preparing the iTfr cells in vitro, which is expected to obtain an enough number of induced follicular regulatory T cells, and provides an important tool and a treatment means for in vitro research of immune tolerance related diseases such as autoimmune diseases and organ transplantation.

The invention discloses a construction method and application of a protein fingerprint spectrum of Tilletia foetida teliospore. The method comprises the following steps: firstly, preparing immunomagnetic beads by using a monoclonal antibody for resisting Tilletia foetida teliospore, wherein the immunomagnetic beads are used for specifically enriching target teliospore in a wheat sample; then, the captured magnetic bead-teliospore compound is placed in a formic acid-acetonitrile solution and subjected to efficient cracking through steel ball grinding, and internal protein of the teliospore is released. The released protein is subjected to MALDI-TOF-MS analysis, so that a protein fingerprint spectrum of the protein is obtained, and a characteristic protein fingerprint spectrum database of the fungal spores is constructed. The rapid identification of the Tilletia foetida teliospore is realized by comparing and analyzing the mass spectrum of the sample to be detected and the self-established database. The detection time is shortened to be within 1 h, the sensitivity reaches up to 105 spores per gram of samples, and the method has the advantages of being simple, convenient, accurate and efficient and is particularly suitable for on-site rapid screening in the field of food quality safety.

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

The invention discloses a plasma or serum exosome separation method which comprises the following steps: firstly, coupling an antibody for resisting four transmembrane protein CD81 on the surface of an exosome by using a magnetic bead to form an immunomagnetic bead; pretreating a serum / plasma sample, enriching exosomes, co-incubating with the immunomagnetic beads, washing the magnetic beads after incubation capture is completed, and washing off uncombined impurities; after being captured, the exosome can be directly cracked, the magnetic beads are removed for downstream experiments, or the magnetic beads are eluted to obtain the complete exosome. The exosome affinity antibody is matched with the magnetic beads, the plasma / serum sample exosome is separated, the unique affinity separation technology not only can remove high-abundance protein in the serum / plasma sample, but also can eliminate other vesicles with similar membrane structures with the exosome, so that the purity of the separated exosome is far higher than that of the exosome obtained by other separation methods.

The present invention relates to the technical field of the detection of ancient silk fabrics, and discloses a preparation method and application of an immunomagnetic bead test strip for rapidly detecting trace proteins in cultural relics, comprising the following steps: (1) after the carboxyl magnetic beads are subjected to a coupling reaction with protein antibodies, the supernatant is removed by magnetic separation, and after washing with a buffer solution and a cleaning agent in sequence, immunomagnetic beads are obtained; (2) the immunomagnetic beads are sprayed on a pretreated nitrocellulose membrane, and after drying, they are reserved for use; the diluted antibody solution I and antibody solution II are respectively sprayed on the test line and the quality control line of the nitrocellulose membrane, and after drying, they are reserved for use; (3) a sample pad, an immunomagnetic bead conjugate pad, a nitrocellulose membrane and a water absorption pad are assembled and cut in sequence to obtain an immunomagnetic bead test strip, which is stored in a dry and sealed manner. The present invention can achieve rapid and sensitive detection while enriching trace proteins, is convenient for operation at the archaeological site, and takes a short time.

The present application relates to the technical field of biotechnology, and particularly relates to a D-dimer chemiluminescence assay kit. The present application provides a D-dimer chemiluminescence assay kit, which comprises a magnetic particle suspension (immunomagnetic beads) coated with D-dimer antibody 1, alkaline phosphatase-labeled D-dimer antibody 2 (enzyme-labeled antibody) and a calibrant. The immunomagnetic beads and the enzyme-labeled antibody are prepared by chemical coupling and stored in a buffer containing a soluble high molecular material, which can effectively improve the detection sensitivity and repeatability. The kit can be used in combination with a substrate solution containing a chromogenic substrate such as adamantane and its derivative AMPPD or APS-5 for a full-automatic immune test system, and the content of D-dimer in plasma or whole blood can be determined by a chemiluminescence instrument. The kit has high detection sensitivity and good repeatability.

This invention discloses a microfluidic product plate for screening tumor cells using immunomagnetic beads, belonging to the field of tumor cell screening technology. It includes a dust-free injection-molded or micro-injection-molded product core plate with a pipeline processing mechanism mounted on it for screening target cells in injected liquid. The pipeline processing mechanism includes an input pipe, several delivery pipes, a first connecting pipe, an electromagnetic adsorption unit, a second connecting pipe, and an output pipe. The input pipe is connected to a sample injection pipe, a sheath flow injection pipe, an immunomagnetic bead injection pipe, and a blood cell recovery pipe. The output pipe is connected to a second sheath flow injection pipe, a waste liquid recovery pipe, and a target cell recovery pipe. The first sheath flow injection pipe, the immunomagnetic bead injection pipe, the blood cell recovery pipe, the electromagnet, and the second sheath flow injection pipe work together to separate impurity cells at the front end of the same product core plate, inject and mix immunomagnetic beads in the middle, and precisely sort target cells at the rear end.

The invention relates to the technical field of micro-fluidic detection equipment, and provides an immunomagnetic bead detection system based on a micro-fluidic chip, the immunomagnetic bead detection system comprises a support and the micro-fluidic chip, and a magnetic field device comprises a magnet; the device is characterized in that the magnet is a cylinder magnetized in the diameter direction, the central axis of the magnet is perpendicular to the bottom face of the reaction bin, the diameter of the magnet is larger than the width of the reaction bin, the diameter of the magnet is smaller than the length of the reaction bin, and the diameter of the magnet is larger than or equal to two times of the width of the reaction bin. The magnetic field intensity of the surface of the magnet ranges from 20 mT to 150 mT; meanwhile, the invention also provides a detection method applying the immunomagnetic bead detection system based on the micro-fluidic chip. The magnetic bead detection system has the beneficial effects that the magnetic beads left in the reaction bin after a user washes the magnetic beads are few, the cleaning effect is good, and the detection limit is reduced; the detection method has the advantages of low detection limit and high detection speed.

The invention discloses an immunomagnetic bead with thermomagnetic dual response, a preparation method of the immunomagnetic bead and application of the immunomagnetic bead in detection of food-borne pathogenic bacteria. The preparation method comprises the following steps: (1) preparation of the magnetic core; (2) construction of the inorganic shell layer; (3) introduction of the temperature-sensitive polymer layer; (4) surface functionalization and streptavidin coupling; organic combination of the thermal response polymer and the magnetic core-shell structure is realized for the first time, so that the immunomagnetic bead has dual response capabilities of temperature control gathering and scattering and magnetic field separation. Under a low-temperature condition, the magnetic beads are kept in a dispersed state, so that target combination is facilitated; after the temperature is raised, the polymer is subjected to hydrophobic collapse, particles are gathered, and rapid capture is realized through a magnetic field, so that the detection efficiency and the binding stability are greatly improved.

The present application relates to a kind of human gastric cancer primary fibroblast separation culture and purification method and its application.The human gastric cancer primary fibroblast separation culture and purification method provided by the present application are simple, efficient.The separation culture method and the purification method provided by the present application can complete tissue dissociation in shorter time, and cooperate multiple immunomagnetic beads to remove miscellaneous cells, so that the gastric cancer primary fibroblast group of stable, relatively consistent morphology adherent growth is obtained, phenotype observation is facilitated, and the interference of miscellaneous cells to subsequent function experiment is reduced.Enriched cells are expanded after culture, and can be used for the subtype research of fibroblast, microtumor environment related function experiment.

The invention relates to the field of CAR-T cell concentration detection, in particular to a method for detecting CAR-T cells through combination of microfluidics and immunofluorescence. Through immunomagnetic bead enrichment and micro-fluidic chip partitioning, CAR-T cells are separated from a blood sample through immunomagnetic beads, then labeling is carried out or enhanced by adding a labeling antigen, anti-TCR-PE and DAPI, three-channel fluorescence labeling is achieved, quantitative analysis can be carried out on the number, the cell morphology and the function of the CAR-T cells through automatic immunofluorescence imaging, and the accuracy of CAR-T cell detection is improved. And the detection limit reaches 0.001%.

The present invention discloses an antibody against Clostridium difficile specific protein, a magnetic bead-antibody conjugate and its application. The monoclonal antibody is any one of monoclonal antibody CD1, monoclonal antibody CD2, monoclonal antibody CD3 and monoclonal antibody CD4. The magnetic bead-antibody conjugate is formed by conjugating a monoclonal antibody with activated carboxyl magnetic beads to obtain the magnetic bead-antibody conjugate. Based on the magnetic bead-antibody conjugate sorting method, antibody magnetic beads that couple and recognize the Clostridium difficile secreted proteins TcdA, TcdB, the highly expressed GDH on the surface of Clostridium difficile, and the LPxTG of Clostridium difficile are used to capture Clostridium difficile, achieving the purpose of rapidly isolating Clostridium difficile from fecal samples. The present invention is based on the immunomagnetic bead separation technology of antigen-antibody reaction for separation, with the characteristics of being rapid, simple, having high enrichment efficiency and strong specificity. The present invention can be used to isolate and remove Clostridium difficile in fecal microbiota transplantation, and can also be used for strain identification and sequencing.

The invention relates to the technical field of cell enrichment and separation equipment, in particular to a specific cell enrichment and magnetic separation system based on immunomagnetic beads. In order to solve the problem that an existing classifier is inconvenient to use, the following technical scheme is provided: a shelving module is mounted on the outer wall of the classifier, a sorting column is placed on a shelving groove of the shelving module, and an auxiliary unit and an adjusting unit mounted in the classifier are mounted in the shelving module; a moving block is installed on the outer wall of the sealing block, a pull rope is installed on the outer wall of the moving block, a sliding block is installed at the other end of the pull rope, and a rotating plate is movably connected to the inner wall of the placing module through a pin shaft. The placement state of the permanent magnet when the permanent magnet is not used and in use is changed, so that magnetic dust particles cannot be adsorbed on the placement module; and after being installed, the separation column can move upwards to adjust the liquid dropping speed, so that the operation difficulty of magnetic separation is reduced.

Provided is a device for screening and separating circulating tumor cells (CTCs), comprising: a chamber module, the chamber module being provided with a mixing chamber, a screening chamber and a waste liquid chamber, and the mixing chamber, the screening chamber and the waste liquid chamber being sequentially communicated; a control module, the control module being mounted on the chamber module, and the control module being configured to control, according to the motion state thereof, whether to discharge a liquid from the mixing chamber to the screening chamber; a temperature control module, the temperature control module being mounted on the chamber module, and the temperature control module being configured to control the temperature in the mixing chamber; and a magnetic enrichment module, the magnetic enrichment module being configured to form a magnetic field in the mixing chamber. Provided is the device for screening and separating CTCs on the basis of immunomagnetic bead separation technology. The device has a compact structure and a small size, is convenient to operate, and has the function of simulating the constant-temperature environment in human bodies to assist in reaction incubation, thereby ensuring the reliability and precision of detection.

The present invention discloses a kit for direct amplification detection of goat pox virus based on single-domain antibody 9‑4‑2 immunomagnetic beads and its application. The kit includes single-domain antibody 9‑4‑2 immunomagnetic beads obtained by coupling the goat pox virus single-domain antibody 9‑4‑2 shown in SEQ ID NO.1 with streptavidin‑biotin and magnetic beads. The present invention uses the single-domain antibody 9‑4‑2 protein purified by gel cutting to couple with magnetic beads to obtain immunomagnetic beads. After the immunomagnetic beads are enriched with goat pox virus, the genomic RNA does not need to be extracted and can be directly amplified. The proposal of the present invention overcomes the phenomenon that goat pox virus is not easy to be found at the peak of virus replication due to the long time of forming the skin, the virus content of the collected diseased material is low, so the detection rate is low, and it is easy to miss the detection, etc.; as well as a series of problems such as time-consuming, costly, and laborious when extracting genomic DNA, and polluting the environment during operation, and it is efficient, sensitive, specific, stable, and practical. The proposal of the present invention provides an efficient technical means for the detection of goat pox virus disease.

The present invention belongs to the field of magnetic separation technology, and in particular, relates to a magnetic separation device and method. The first tooth tip and the tip of the second tooth tip of the magnetic separation device are arranged parallel to each other and form a tip installation gap; a separation channel with an outlet and an inlet is provided on the microfluidic chip; the extension direction of the first tooth tip is arranged parallel to the length direction of the replaceable microfluidic chip and the separation channel; the microfluidic chip is detachably installed in the tip installation gap, and the two opposite outer walls of the microfluidic chip are fitted with the tip of the first tooth tip and the tip of the second tooth tip; when the sample liquid flows into the inlet of the separation channel located in the tip installation gap, the high gradient magnetic field generated between the first tooth tip and the second tooth tip causes the immunomagnetic beads in the sample liquid to be adsorbed on the two opposite inner walls corresponding to the first tooth tip and the second tooth tip in the separation channel. The present invention has a simple structure and production, low cost, and improves the accuracy, separation purity and separation efficiency of magnetic separation.

The application discloses a kind of for visualizing based on immunomagnetic bead detection staphylococcus aureus kit, preparation carboxylated Fe3O4 Nano magnetic beads, the magnetic bead is coupled with anti staphylococcus aureus chicken yolk antibody IgY, and immunomagnetic bead is obtained;Take the liquid to be measured, it is mixed with immunomagnetic bead, is suspended after room temperature on rotating mixer, and is magnetically separated, then appropriate amount of H2O2 Solution is added, and is magnetically separated, and the remaining H2O2 Solution is added 50 μL HRP solution and 50 μL TMB solution color development, and color change of solution can be qualitatively observed with naked eye.The application provides the use of immunomagnetic bead and TMB-HRP-H2O2 color development system combination staphylococcus aureus detection, fast, visual detection staphylococcus aureus, shorten detection time, and the minimum detection concentration is 10 3 CFU·mL ‑1 , with the recovery rate of 102.26%, high sensitivity, good stability, strong specificity.

The application discloses a nucleic acid free extraction reagent of plasma exosome and a direct PCR amplification kit. The nucleic acid free extraction reagent comprises 0.001-0.1% polidocanol and 1-100 mM Tris-HCl in mass volume concentration, and the solvent is water. The direct PCR amplification kit provided by the application comprises the nucleic acid free extraction reagent and an immunomagnetic bead suspension, a washing liquid, a free extraction reagent and a PCR Mix solution; the surface of the immunomagnetic bead is simultaneously coated with three exosome specific antibodies of CD9, CD63 and CD81, so that the recovery rate of the exosome can be greatly improved during the extraction and purification of the plasma exosome; the PCR Mix solution contains an anti-inhibitory component, can effectively neutralize the inhibitors such as proteins in the PCR system, and ensures the amplification efficiency of the PCR. The nucleic acid free extraction reagent of the application can realize the amplification detection of the exosome nucleic acid free extraction, and simplifies the operation process of the PCR detection.

The invention provides a cell immunomagnetic bead sorting device, and belongs to the technical field of magnetic bead sorting, the cell immunomagnetic bead sorting device comprises a U-shaped containing frame, one end of a telescopic piece is connected with a moving plate, one end of the moving plate is rotatably connected with the U-shaped containing frame, one end of the U-shaped containing frame is rotatably provided with a rotating rod, and the rotating rod is rotatably connected with a support. A first fixing frame is slidably installed in the U-shaped containing frame, a containing plate is fixedly installed on the first fixing frame, a magnet is slidably installed on one side of the U-shaped containing frame, a connecting rod is fixedly installed at one end of the first fixing frame, and a trapezoidal block is slidably installed on the connecting rod. And a wedge-shaped block is slidably installed on the inner side wall of the U-shaped containing frame, a first spring is installed between the wedge-shaped block and the inner side wall of the U-shaped containing frame, through back-and-forth movement of the telescopic part, mixed liquid in the test tubes on the containing plate can be fully and evenly mixed, and the situation that due to the fact that the mixed liquid is left on test tube covers, sorting is not thorough can be avoided.

The invention belongs to the technical field of microbiological detection and molecular diagnosis, and discloses a primer group, a kit and a detection method for detecting salmonella enteritidis, the kit comprises an immunocapture component, an isothermal amplification component, a CRISPR / Cas12a detection component and auxiliary components; the isothermal amplification component is any one of an RPA (recombinase polymerase amplification) component or an LAMP (loop-mediated isothermal amplification) component, the RPA component comprises a specific primer group targeting 86th to 212th nucleotides in a salmonella enteritidis hilA gene coding region, and the LAMP component comprises a specific primer group targeting 58th to 250th nucleotides in the salmonella enteritidis hilA gene coding region. The primer group, the kit and the detection method for detecting the salmonella enteritidis successfully integrate three key technologies of RAP and LAMP rapid isothermal amplification, CRISPR / Cas12a high-specificity recognition and trans-cleavage and immunomagnetic bead targeted enrichment, and creatively provide the primer group, the kit and the detection method for detecting the salmonella enteritidis.

The present invention discloses a monoclonal antibody against Avibacterium paragallinarum, immunomagnetic beads labeled therewith and applications thereof. The monoclonal antibody comprises a light chain variable region and a heavy chain variable region, the amino acid sequence of HCDR1 of the monoclonal antibody is shown in SEQ ID NO:9, the amino acid sequence of HCDR2 is shown in SEQ ID NO:10, the amino acid sequence of HCDR3 is shown in SEQ ID NO:11, the amino acid sequence of LCDR1 is shown in SEQ ID NO:3, the amino acid sequence of LCDR2 is shown in SEQ ID NO:4, and the amino acid sequence of LCDR3 is shown in SEQ ID NO:5. The monoclonal antibody has high titer and strong specificity, and is suitable for the preparation of immunomagnetic beads based on the monoclonal antibody. The prepared immunomagnetic beads have a high capture rate, strong specificity and sensitivity for Avibacterium paragallinarum.

The invention discloses a multiple sclerosis antigen-specific regulatory T cell as well as an induction and amplification method and application thereof, and belongs to the technical field of biomedicine. In order to solve the problems that multiple sclerosis (MS) antigen-specific CD4 + regulatory T cells cannot be induced and amplified at present and other antigen-specific CD4 + regulatory T cell induction methods are low in amplification efficiency, low in amplification algebra, low in obtained cell functionality and the like, a B cell activated by sCD40L is used as an antigen presenting cell, and a CD4 + initial T cell sorted by immunomagnetic beads is induced and amplified into Treg; further, the MS antigen specificity Treg is obtained, and a foundation is laid for cell therapy of MS. The induction amplification method provided by the invention has the advantages of rapidness, simplicity, high amplification efficiency, strong functions of obtained cells, high purity after flow sorting and the like, and the obtained MS antigen specific Treg can be used for clinical cell therapy and can be popularized to treatment of other autoimmune diseases.

The invention belongs to the technical field of immune cell detection, and particularly relates to a detection reagent for immune cells and a preparation and detection method of the detection reagent. S2, carrying out enzymolysis digestion; s3, preparing a single-cell suspension; s4, sorting the immunomagnetic beads; s5, carrying out flow dyeing; s6, assembling the kit; according to the immune cell detection reagent and the preparation and detection method, through the synergistic effect of different enzymes, tissues can be decomposed mildly and effectively, the excessive digestion situation possibly caused by only using a certain enzyme is prevented, damage to cell activity is reduced, the activity requirement of cells in subsequent detection is guaranteed, and then the accuracy of a detection result is guaranteed; secondly, various enzymes are used for decomposing different components in the tissue, so that the tissue can be digested into a single-cell suspension more quickly and thoroughly compared with a single enzyme, the cell yield is increased, and enough cells are provided for the subsequent steps of immunomagnetic bead sorting and the like.

The present invention belongs to the field of immunology and detection technology, and specifically relates to a microfluidic chip with a flow channel switching valve. The chip comprises a cleaning liquid flow channel, a buffer flow channel, a waste liquid flow channel, a first mixing flow channel, a second mixing flow channel, a flow channel switching valve, a detection zone, a first waste liquid tank, a second waste liquid tank, a sample well, an immunomagnetic bead injection device, and a fluorescent antibody injection device. The cleaning liquid flow channel, the buffer flow channel, the waste liquid flow channel, the first mixing flow channel, and the second mixing flow channel are respectively connected to the flow channel switching valve, the second end of the second mixing flow channel is connected to the inlet of the detection zone, and the outlet of the detection zone is connected to the second waste liquid tank. The height of the detection zone is less than the spacing between adjacent magnetic bead, sample, and antibody mixed microspheres. Using the microfluidic chip of the present invention for antigen concentration detection can simplify the operating steps of the entire process, improve the efficiency of antigen concentration detection, and greatly save the production cost of the microfluidic chip.

This invention discloses a detection kit for determining prolactin (PRL), comprising immunomagnetic beads, an oxidant, and a surfactant. The oxidant is a peroxidant, and the surfactant is an anionic surfactant. This detection kit for PRL utilizes an oxidant to cleave interfering substances and a surfactant to encapsulate the cleaved substances. This improves the kit's detection sensitivity, enhances its anti-interference ability when testing clinical samples, significantly improves specificity, and greatly increases clinical concordance rate. It solves the problem of falsely elevated results caused by interference from interfering substances during PRL determination.

This invention belongs to the field of biomedical technology and provides a high-throughput drug screening system and its usage method for enriching circulating tumor cells (CTCs) in liver cancer. It includes an information processing and control unit; a diseased liver perfusion device comprising an organ chamber containing injection fluid at the bottom, an arterial supply line, an arterial end sensor, an arterial end clamp valve, at least two membrane lungs, a portal vein supply line, a portal vein end clamp valve, and a portal vein end sensor; a CTC enrichment device, which uses erythrocyte lysis to remove erythrocytes and immunomagnetic bead labeling to remove leukocytes, enabling the processing of large volumes of perfusion fluid in a single step to obtain enriched circulating tumor cells; a high-throughput drug screen; and phototubes, arterial end sensors, and portal vein end sensors, each electrically connected to the information processing and control unit. Compared with existing technologies, the high-throughput drug screening system and its usage method of this invention have the advantages of establishing a room-temperature mechanically perfused ex vivo liver cancer model, realizing the processing of large volumes of liquid specimens, and providing automated high-throughput drug screening functionality.