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Gastrin-17 enzymatic chemiluminescence immunoassay kit

A technology of enzymatic chemiluminescence and detection kits, applied in biological testing, measuring devices, scientific instruments, etc., can solve the problems of gastric cancer screening, such as difficulties, large errors, and cumbersome procedures

Inactive Publication Date: 2015-09-16
BIOHIT BIOTECH HEFEI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the G-17 detection kits currently on the market are mainly based on the traditional ELISA method, which requires a lot of manual operations, the process is cumbersome, and the error is large, which brings great difficulties to clinical gastric cancer screening.

Method used

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  • Gastrin-17 enzymatic chemiluminescence immunoassay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0116] Step 1: Preparation of Immunomagnetic Beads Coated with Gastrin-17 Monoclonal Antibody

[0117] 1. Preparation of Magnetic Bead Activation Buffer:

[0118] 1. Preparation of initial wash buffer:

[0119] 1.1 Weigh 0.1952g of MES hygrate into 100ml of pure water and adjust the pH to 5.0.

[0120] 1.2 Add 50 μl of Tween-20 to prepare 0.01 mol / L MES buffer, and adjust the pH to 5.0.

[0121] 2. Preparation of coupling buffer:

[0122] 2.1 Weigh 0.38138g Na 2 B 4 O 7 ·10H 2 O was dissolved in 40 ml of pure water to obtain a borax solution of 0.05 mol / L.

[0123] 2.2 Weigh 0.1237g H 3 BO 3 Dissolve in 10 ml of pure water to obtain 0.2 mol / L boric acid solution.

[0124] 2.3 Take 18ml of 0.05mol / L Na 2 B 4 O 7 Solution with 2ml of 0.2mol / L H 3 BO 3 The solutions were mixed, and the volume was adjusted to 80 ml to obtain 0.05 mol / L boric acid buffer.

[0125] 3. Preparation of final lotion:

[0126] 3.1 Weigh 2.78g Na 2 HPO 4 Dissolve in 100ml pure water to ge...

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Abstract

The invention discloses a gastrin-17 enzymatic chemiluminescence immunoassay kit and belongs to the technical field of chemiluminescence immunoassay analysis. The kit comprises an enzyme label liquid, a gastrin-17 standard, gastrin-17 monoclonal antibody-coated immunomagnetic beads, a sample diluent, a chemiluminescent substrate liquid and a washing liquid. The principle of the gastrin-17 enzymatic chemiluminescence immunoassay kit comprises that a gastrin-17 monoclonal antibody is connected to the surface of a magnetic bead so that a solid phase agent is obtained, and through capture of gastrin-17 in a sample and use of an enzyme-labeled anti-gastrin-17 monoclonal antibody, a solid phase-antibody-antigen-enzyme-labeled antibody sandwiched immune complex is formed. Through combination of a chemiluminescence technology and an immunomagnetic bead technology, the prepared kit has the advantages of high sensitivity, good specificity, wide linearity range and good stability and can satisfy clinical requirements on stomach function detection.

Description

technical field [0001] The invention belongs to the technical field of immunodetection analysis, in particular to a gastrin-17 enzymatic chemiluminescence detection kit. Background technique [0002] Recent studies have confirmed that serum gastrin-17 (Gastrin-17, G-17) content is an important indicator of early gastric cancer. G-17 is a gastrointestinal hormone secreted by G cells in the gastric antrum and is a stimulating hormone for gastric acid secretion. It is a sensitive indicator reflecting the secretory function of gastric antrum and plays an important role in the diagnosis and screening of atrophic gastritis and gastric cancer. The determination of G-17 is helpful to judge the existence of atrophy and its distribution location and degree. In patients with gastric body atrophy, oxyntic glands are reduced, and the stomach presents a state of low gastric acid, which leads to an increase in serum gastrin G17 level; in gastric antrum atrophy, the number of G cells decr...

Claims

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Application Information

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IPC IPC(8): G01N33/74
CPCG01N33/74G01N2800/062G01N2800/7028
Inventor 王明丽洪叶赵俊刘峰
Owner BIOHIT BIOTECH HEFEI
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