287 results about "Antibody antigen" patented technology

The present invention includes compositions and methods for increasing the effectiveness of antigen presentation using a DCIR-specific antibody or fragment thereof to which an antigen is attached that forms an antibody-antigen complex, wherein the antigen is processed and presented by a dendritic cell that has been contacted with the antibody-antigen complex.

This document provides methods and materials involved in binding a binder (e.g., an antibody, antigen binding fragment, and / or antibody domain) to a SARS-CoV-2 antigen. For example, binders (e.g., antibodies, antigen binding fragments, and antibody domains) that bind to a SARS-CoV-2 polypeptide and methods and materials for using one or more such binding molecules to treat a mammal (e.g., a human) having COVID-19 (or a viral infection caused by SARS-CoV-2) are provided.

The present invention discloses a quantum point labeled sandwich immunodetection method and its diagnosis kit. It is a new type sandwich immunodetection method using QDs nano particle as label to make antigen antibody specificity sandwich reaction. It includes the following processes: firstly, directly or indirectly enveloping captured antibody in microwell of polystyrene plate, forming captured antibody-antigen-detection antibody three-layer sandwich luminescent immune complex and fluorescence intensity detection. According to that every QD has narrow and symmetrical fluorescence spectral peak it can select and use quantum point label needle sending different light to simultaneously detect several antigens to be tested in same sample.

The present invention discloses a protein transistor device, wherein an antibody molecule (antibody-antigen) is bonded to at least two gold nanoparticles in a high reproducible self-assembly way to form molecular junctions, and wherein the two gold nanoparticles are respectively joined to a drain and a source. The protein transistor device can be controlled to regulate current via applying a bias to the gate. The conformational change of the protein molecule will cause the variation of the charge transport characteristics of the protein transistor device. The protein transistor device can be further controlled by different optical fields via conjugating a quantum dot to the molecular junctions. Therefore, the present invention has diversified applications.

Specific binding members comprising human antibody antigen binding domains specific for human transforming growth factor beta (TGFβ) bind specifically isoforms TGFβ2 and TGFβ1 or both, preferentially compared with TGFβ3. Specific binding members may be isolated and utilized in the treatment of disease, particularly fibrotic disease and also immune / inflammatory diseases. Therapeutic utility is demonstrated using in vitro and in vivo models. Full sequence and binding information is provided, including epitope sequence information for particularly advantageous specific binding member which binds the active form of TGFβ2, neutralizing its activity, but does not bind the latent member.

An integrated chromatography-immunoassay system for integrated chromatography-immunoassay system includes a chromatographic unit that receives labeled nano-structured probes comprising nano particles and antibodies attached to the nano particles, and a test membrane comprising coating antigens. The chromatographic unit allows the labeled nano-structured probes to diffuse there through and into the test membrane, wherein the antibodies on the nano particles are bound to the coating antigens. A laser device emits a laser light to illuminate the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane. A spectral analyzer obtains a Raman spectrum from light scattered from the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane, and to identify a spectral signature in the Raman spectrum associated with the antibody-antigen pair, which enables detection and identification of the antibody.

The present invention includes compositions and methods for increasing the effectiveness of antigen presentation using a DCIR-specific antibody or fragment thereof to which an antigen is attached that forms an antibody-antigen complex, wherein the antigen is processed and presented by a dendritic cell that has been contacted with the antibody-antigen complex.

A method for enhancing fluorescence of a biomolecule includes the step of associating the biomolecule having intrinsic fluorescence with a sensing surface that contains nanostructured metal. Association of the biomolecule with the nanostructured metal enhances its intrinsic fluorescence, which is detected upon exposure to electromagnetic radiation of a suitable wavelength. The sensing surface may include capture or ligand molecule which binds to the biomolecule and sequesters it in proximity to the nanostructured metal, thereby causing its fluorescent signal to be enhanced. The method can be used in label-free bioassays for detection of interacting biomolecules, such as antibody-antigen binding.

An integrated chromatography-immunoassay system for integrated chromatography-immunoassay system includes a chromatographic unit that receives labeled nano-structured probes comprising nano particles and antibodies attached to the nano particles, and a test membrane comprising coating antigens. The chromatographic unit allows the labeled nano-structured probes to diffuse there through and into the test membrane, wherein the antibodies on the nano particles are bound to the coating antigens. A laser device emits a laser light to illuminate the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane. A spectral analyzer obtains a Raman spectrum from light scattered from the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane, and to identify a spectral signature in the Raman spectrum associated with the antibody-antigen pair, which enables detection and identification of the antibody.

Microfluidic devices for use with reagents bound to microspheres for determination of the concentration of an analyte in a liquid sample are provided. The devices include two sequential mixing channels that promote rapid binding of microsphere-bound reagents with reagents in solution and a means for detecting labeled microsphere-bound reaction products. Also provided are methods for using the devices with microsphere-bound reagents to determine the concentration of an analyte in a liquid sample and to measure the binding affinity of antibody for an antigen.

The invention discloses an immune base preparation method and an antigen or antibody immunoassay method. According to the immune base preparation method, a silicon slice is modified by using gold or silver nanoparticles, and antibodies / antigens are modified on the surfaces of the gold or silver nanoparticles, thereby being used for capturing antigens / antibodies to be assayed. According to the antigen or antibody immunoassay method, an immune base which is prepared by using the immune base preparation method and gold or silver nanoparticle immunoprobes is used, an immune base-antigen / antibody-immunoprobe three-layer sandwich structure is formed through an antigen-antibody immune complex reaction, and the assay on the antigens / antibodies to be assayed is realized in a manner that the characteristic dactylograms of Raman markers on the surfaces of the immunoprobes are assayed by using a surface-enhanced Raman scattering effect of the gold or silver nanoparticles. The method has the advantages that the sensitivity of the immunoassay can be greatly improved and the assay on high-flux multiple antigens / antibodies can be carried out.

There is provided a system and process for detecting biomolecular interaction on a substrate having a biomolecule immobilized on a surface of the substrate. The system and process incorporate a scanning Kelvin microprobe (SKM) capable of analyzing surface topography as well as a contact potential difference image signal. Also provided is the use of SKM in measuring and analyzing biochemical molecular interactions between a probe bound to the surface of the substrate, and a target suspected to be present in a liquid sample. One of the probe and target combination is a biomolecule such as a nucleic acid, a polypeptide, or a small molecule, and an antibody antigen combination may be used.

The invention discloses a gastrin-17 enzymatic chemiluminescence immunoassay kit and belongs to the technical field of chemiluminescence immunoassay analysis. The kit comprises an enzyme label liquid, a gastrin-17 standard, gastrin-17 monoclonal antibody-coated immunomagnetic beads, a sample diluent, a chemiluminescent substrate liquid and a washing liquid. The principle of the gastrin-17 enzymatic chemiluminescence immunoassay kit comprises that a gastrin-17 monoclonal antibody is connected to the surface of a magnetic bead so that a solid phase agent is obtained, and through capture of gastrin-17 in a sample and use of an enzyme-labeled anti-gastrin-17 monoclonal antibody, a solid phase-antibody-antigen-enzyme-labeled antibody sandwiched immune complex is formed. Through combination of a chemiluminescence technology and an immunomagnetic bead technology, the prepared kit has the advantages of high sensitivity, good specificity, wide linearity range and good stability and can satisfy clinical requirements on stomach function detection.

The present invention relates to a recombinant human antibody comprising an antibodysequence specific for the MSP-3 antigen of Plasmodium falciparum. In particular, the invention relates to a recombinant human antibody which is specific for the MSP-3194-257 antigen. The invention further relates to nucleic acid encoding such antibodies and to uses of these antibodies, in particular in the treatment or prophylaxis of malaria.

The present invention provides methods for detecting a target nucleic acid using a circular dual-hairpin probe that is formed upon the presence of the target nucleic acid. The detection methods find use in detecting the presence of antibody-antigen complexes and for detecting the binding of a ligand to its binding partner. Kits and reaction mixtures for performing the present methods are also provided.

The present invention relates to a dry-type immunoassay test strip and a preparation method and application thereof. On a plastic substrate of the test strip is sequentially stuck with a sample pad, a nitrocellulose membrane anda water absorption pad from left to right, wherein the right end of nitrocellulose membrane is provided with a control line and a detection line that are parallel to each other; the left end of nitrocellulose membrane is provided with a tracer particle labeled antibody / antigen-coated line and a closed protein line I, wherein the closed protein line I is formed by coating closed protein solution I with a nitrocellulose membrane, and the antibody / antigen-coated line is formed by spraying the tracer particle labeled antibody / antigen on a closed protein line II, and the closed protein line II is formed by coating closed protein solution II with a nitrocellulose membrane. . The test strip can be applied in the immunoassay based on the double antibody sandwich method or the competitive method, and has advantages of simple operation, rapid chromatography, high specificity, accurate test results and strong stability.

The present invention relates to a measurement method of immune colloidal gold particle fluorescence quenching in the field of detection technology. The antibody or antigen of target compound, namely tested compound is connected on the microgranule or microwell to form solid-phase carrier, the tested sample is added, and excess colloidal gold lag also is added, so that the target compound in the sample and the antibody or antigen fixed on the microgranule or microwell plate are undergone the immunological reaction to form solid-phase carrier compound of antibody antigen, namely conjugated compound and unreacted colloidal gold lag, namely free compound, then the conjugated compound and free colloidal gold tag are separated, in the free compound a fluorescence substance is added to make fluorescence signal be quenched. The signal quenching extent is related to free gold lag quantity in the system, and the quantity of free compound is related to quantity of target compound, so that it can implement quantitative detection of target compound.

The present invention discloses an antibody / antigen detection method based on mobile equipment. The method comprises: 1) determining the excitation signal parameter of a target antibody or antigen; 2) acquiring a reaction unit, wherein the reaction unit comprises an electrode pair spread on the bottom portion of the reaction unit; 3) acquiring auxiliary detecting control modules, wherein the modules comprise an excitation signal conditioning module; 4) sampling and pretreating a body fluid sample to be detected; 5) applying an excitation signal and sampling an impedance value; and 6) carrying out data analysis to obtain the detection result, wherein mobile intelliegnt equipment is used to read the impedance value stored in the auxiliary detecting control module, the read impedance value is analyized through the detecting control software arranged in the mobile intelliegnt equipment so as to qualitatively obtain the detection result, and the detection result is uploaded to the cloud terminal. The method of the present invention has advantages of easy operation, rapid detection, high detection precision, and the like.

The invention relates to a detection kit for interleukin 6. The detection kit comprises two specific monoclonal antibodies of the interleukin 6; one monoclonal antibody covering a perforated plate, the other monoclonal antibody labeled by biotin and the interleukin 6 of a sample to be detected form a sandwich compound structure of covering antibody-antigen-biotin labeled antibody; the biotin labeled on the antibody combines with streptavidin labeled by HRP, and then the HRP reacts with a substrate to generate a signal which is used for conducting quantitative analysis on the interleukin 6. The detection kit can quantitatively detect the content of the interleukin 6 in the sample rapidly and sensitively.

The present invention provides a method for detecting autoantibodies in a subject which reacts with a GP73 antigen. Increased levels of GP73-specific autoantibodies in a sample from the subject which bind to GP73 antigen are indicative of liver disease in the subject.

The invention discloses a captured-antibody competition sandwich immunodetection method capable of extending detection scope and a biosensor. The captured-antibody competition sandwich immunodetection method is utilized for detecting the concentration of a to-be detected antigen, concretely, the antigen in a to-be detected sample is firstly reacted with a dissociated captured antibody and a marked antibody, and then reacted with an immobilized captured antibody, so that only one complex of marked antibody-antigen-immobilized captured antibody in formed immunization complexes can be detected, and the antigen concentration is determined by measuring the mark signal on the marked antibody of the immunization complex. Because the dissociated captured antibody-antigen-marked antibody complex and the dissociated captured antibody-antigen complex cannot be detected by a detector, the purpose of indirectly diluting the antigen concentration in the to-be detected sample is realized, and the detection scope of the antigen concentration of the to-be detected sample is expanded. Also, the biosensor prepared by utilizing the method has the advantages of wide detection scope, high sensitivity and short detection time.

A microparticle immune chromatography of time-resolution fluorescence emulsion includes preparing biotinylation antibody and preparing immune time-resolution fluorescence microparticles, using Fusion 5 film to prepare avidin solid phase film detection line and quality control line by utilizing avidin to envelop said film, finally using double-antibody sandwich method to detect antigen quickly.

The present system and methods allow for low level detection of as little as single pathogen particles, such as viral or bacterial particles, during the early stage of infection. An optical trapping system, such as laser tweezers, are used to trap a substrate to which an analyte has been bound to detect and record the thermal motion of an antibody-antigen interaction that may occur between an anti-viral antibody-coated microsphere and a viral particle for example. The system may be equipped with a detection system such as a position sensitive photodetector (PSD) to record the thermal motion of a trapped microsphere and particle at a certain frequency. The thermal motion data may be Fourier transformed into a power spectrum, which may be transformed into an output value using a Lorentzian equation. The power spectrum of the trapped microsphere may be recorded before and after binding of the pathogenic particle to determine the presence thereof.

The present invention provides a novel method called Sequential IMmunoPeroxidase Labeling and Erasing (SIMPLE) that enables the simultaneous visualization of at least five markers within a single tissue section. Utilizing the alcohol-soluble peroxidase substrate 3-amino-9-ethylcarbazole (AEC), combined with a rapid non-destructive method for antibody-antigen dissociation, the present application discloses the ability to erase the results of a single immunohistochemical stain while preserving tissue antigenicity for repeated rounds of labeling. The present invention also provides methods for visualizing multiple antigens simultaneously.

The invention relates to a preparation method of a water-insoluble drug microcapsule. The water-insoluble drug microcapsule is a capsule with a multi-layer shell structure, and the wall of the water-insoluble drug microcapsule consists of a water-insoluble drug and a membrane layer formed by alternating deposition of a plurality of layers of polyelectrolyte with opposite charges. The preparation steps are as follows: 1), at first, preparing a soluble template microsphere with the surface thereof absorbed with a plurality of polyelectrolyte membranes; 2) then, removing the soluble template microsphere to prepare a hollow polyelectrolyte capsule; and 3), finally, loading the water-insoluble drug in the hollow polyelectrolyte capsule. The insoluble drug microcapsule prepared in the method has the advantages of uniform size, high drug solubility, controllable drug releasing rate and good dispersibility in various kinds of aqueous solution, and can also be connected with biologically active substances, such as antibodies, antigens, enzymes, proteins or nucleic acid, in order to achieve drug targeting.

The present invention discloses an assay for determining the presence of an anti-PEG antibody in a biological sample. Embodiments according to this aspect of the present invention will generally have the steps of: (1) providing an antigen probe capable of forming an antibody-antigen complex with the anti-PEG antibody; (2) contacting the biological sample with the antigen probe under conditions favorable for formation of the antibody-antigen complex; and (3) analyzing the antigen probe, after having performed step (2), to detect for the presence of the antibody-antigen complex, wherein the presence of the anti-PEG antibody is determined if the antibody-antigen complex is detected. Also disclosed are methods for screening patients, methods for monitoring patients using assays of this invention and kits for performing thereof.

An assay test solution, a method for using, and an apparatus for the rapid detection of multiple pathogens using a FRET-based phenomenon. A volume of fluid, possibly containing pathogens, is passed through an intake and combined with an assay solution of quantum dot / antibody-antigen / quencher complexes that dissociate and recombine with the pathogens into quantum dot / antibody-pathogen complexes. The quantum dot / antibody-antigen / quencher and quantum dot / antibody-pathogen complexes are captured on a detection filter which is illuminated by a light source. The quantum dot / antibody-pathogen complexes, but not the quantum dot / antibody-antigen / quencher complexes, fluoresce when excited by the light from the light source and the fluorescence is picked up by a photodetector, indicating the presence of the pathogens.

The invention especially relates to a procalcitonin detection kit, and a method of measuring content of procalcitonin therewith. The procalcitonin detection kit includes a reagent R1 and a reagent R2, wherein the reagent R1 is a reaction liquid formed by mixing various surfactants and used for promoting antibody-antigen specificity reaction, and the reagent R2 is a nano-latex particle buffer liquid of an anti-human procalcitonin antibody and contains 0.1-2% of nano-latex particles marked by the anti-human procalcitonin antibody. The kit is 0.12 ng / ml in detection sensitivity which is higher than analytic sensitivities of other like products. The nano-latex particle-type procalcitonin detection kit is high in sensitivity, good in specificity, high in accuracy and good in precision, wherein latex particles in different particle sizes are crosslinked with a polyclonal antibody to form conjugates in different particle sizes, which are then mixed according to different ratio, so that the kit is increased in linearity scope and is improved in detection sensitivity.

The invention discloses an integration micro-fluidic chip for immune analysis research. The chip is mainly composed of a concentration gradient generation unit, an equal allocation unit and a micropore reaction chamber matrix with the diameter of 5.8mm and the depth of 100 mu, and has small volume and large specific surface area; the arrangement of the micropore reaction chamber matrix is consistent with the arrangement of a traditional porous plate, and the micropore matrix can be applied to a standard microplate reader for detecting and gathering analysis data. According to the invention, the operations of absorption and fixation of antibodies, antigens and enzyme during immune analysis operation, the preparation of sample solutions with different concentrations, liquid replacement, washing and the other operations are all integrated on a chip and are completed automatically according to the process creatively; the reagent consumption can be lowered obviously; the analysis time can be shortened; and the efficiency can be improved. Compared with the traditional porous plate technology, according to the invention, the complex manual operations of sample solution preparation, washing and the like are omitted, and the integration micro-fluidic chip for immune analysis research is compatible with the standard microplate reader on the detecting method.

Belonging to the technical field of immunodetection and analysis, the invention specifically provides a myoglobin enzymatic chemiluminescence immunodetection method. The method includes the steps of: forming a solid phase-antibody-antigen-enzyme-labeled secondary antibody immune sandwich composite and detecting the immune sandwich composite by means of chemiluminescence. The invention also provides a myoglobin enzymatic chemiluminescence immunodetection kit. The detection method and the detection kit provided in the invention are suitable for detection and analysis of myoglobin, and have the advantages of large detection range, and high sensitivity and specificity.