14 results about "Horse-radish" patented technology

The invention relates to a bioelectrochemical sensor used for detecting hydrogen peroxide and a manufacturing method of the bioelectrochemical sensor. The novel bioelectrochemical sensor is a sensor with a three-electrode system, wherein the counter electrode is a platinum electrode, the reference electrode is saturated calomel electrode and the working electrode is gold electrode. The sensor is based on orientation combination of a polypeptide horse radish peroxidase with characteristic sequence, the promotion effect of the polypeptide horse radish peroxidase on the activity of the enzyme and the characteristic of high sensitivity of electrochemical detection methods. The sensitivity of hydrogen peroxide sensors is effectively improved and satisfactory results are obtained.

Nucleotide compositions, recombinant vectors, and recombinant viral genomes are disclosed that include a modified horseradish peroxidase (HRP) gene sequence, as well as recombinant viruses that have the modified HRP gene sequence incorporated into the viral genome thereof. The recombinant viruses are utilized in an HRP-based assay to analyze the neutralization potential of candidate antiviral agents, such as antiviral antibodies.

The application discloses a high-stability horseradish peroxidase, a recombinant expression vector, a preparation method and application. The amino acid sequence of the horseradish peroxidase is shown as SEQ ID NO. 1. Compared with wild-type horseradish peroxidase, the enzyme activity of the horseradish peroxidase is increased by 3.2 times, and compared with commercially available horseradish peroxidase, the thermal stability is better. The method can produce the horseradish peroxidase by using a tobacco transient expression system. Compared with an Escherichia coli expression system and a yeast expression system, the tobacco transient expression system can correctly fold and glycosylate, and has higher enzyme activity and stability. The purity of the horseradish peroxidase obtained according to the method can reach RZ 3.2, the enzyme activity can reach 275 U / mg, and the horseradish peroxidase can be applied to immunological detection.

This invention discloses a dry chemical reagent for total cholesterol detection, comprising a diffusion layer, a spacer layer, and a reagent layer sequentially overlapped. The diffusion layer includes reflective particles, a binder, a surfactant, cholesterol esterase, and cholesterol oxidase. The reagent layer includes a carrier, horseradish peroxidase, an indicator composition, and a buffer solution. The dry chemical detection reagent of this invention employs a coating method, layering a premixed homogeneous slurry onto a support layer. A spacer layer blocks interlayer interference, and phenolic substances replace photosensitizers as chromogens, combined with a specific ratio of substrate and buffer system. The multi-layered, spacer-free, low-interference vertical detection system constructed by this invention significantly improves the repeatability, accuracy, and stability of the detection reagent.

The application provides a method for in-situ encapsulating enzymes based on a hydrophilic conductive layered double hydroxide, and realizes immobilization of horseradish peroxidase. The immobilized enzyme system exhibits significant electrochemical performance advantages, and can effectively promote electron transfer in the enzyme reaction process, and reaches reaction equilibrium faster than free enzymes and traditional encapsulation systems. Benefited from the enzyme-friendly environment provided by the hydrophilic structure of the layered double hydroxide, the encapsulated enzyme can maintain a favorable conformation and enhance stability, and its reusability is also improved due to the protection of the carrier. Based on the high activity and high stability of the immobilized enzyme, a portable electrochemical sensor is developed for detection of hydrogen peroxide in human urine samples. The electrochemical platform provides a new path for diabetes monitoring, has the outstanding advantages of rapid response, strong anti-interference, good portability, low cost and the like, and the detection range reaches 50 pM-250 muM, and the detection limit is as low as 36.96 pM.

This invention provides a multiplex fluorescence immunohistochemistry method. The method employs a horseradish peroxidase (HRP)-labeled primary antibody in combination with an HRP inactivating agent, avoiding multiple rounds of elution and counterstaining of tissue sections. This shortens the process and time, enabling simultaneous multiplex fluorescence labeling of five or more color channels within two days, and achieving single-cell resolution for single-cell segmentation.

The application belongs to the technical field of nanomaterials, and provides a CaPhy-NAD nanoenzyme, a preparation method, application and an alcoholism treatment preparation thereof. The CaPhy-NAD nanoenzyme is prepared by using calcium phytate nanoparticles as a carrier and in-situ loading of ethanol oxidase, acetaldehyde dehydrogenase, horseradish peroxidase and coenzyme NAD. 2+ The CaPhy-NAD nanoenzyme can effectively activate the catalytic activity of acetaldehyde dehydrogenase, and the three enzymes can have a high-efficiency confined cascade reaction in the pore channel of the calcium phytate nanoparticles, so that the recycling of the coenzyme NAD is realized, and the three types of alcohol metabolism-related toxic substances, i.e., ethanol, acetaldehyde and hydrogen peroxide, can be simultaneously removed. The CaPhy-NAD nanoenzyme prepared by the application has excellent catalytic activity and good biological safety, and can be orally administered, thereby providing a new technical idea and solution for the prevention and treatment of alcoholism.

The present application relates to the field of food and medicine, and particularly relates to a horse radish and / or extract thereof, a composition, a medicine box or a combination product for myopia prevention and control. The horse radish and / or extract thereof can effectively prevent and control myopia, and when combined with atropine sulfate, the concentration of atropine sulfate can be significantly reduced, side effects such as asthenopia are reduced, and the safety of the medicine is improved.

This invention provides an orange-yellow fluorescent silicon nanoparticle, its preparation method, and its application. Using N-[3-(trimethoxysilyl)propyl]ethylenediamine (DAMO) as the silicon source and 4,6-diaminoresorcinol dihydrochloride as the reducing agent, water-soluble orange-yellow fluorescent silicon nanoparticles are prepared through a room-temperature reaction. The preparation method of this invention is simple and mild. The prepared fluorescent silicon nanoparticles can be used for the detection of horseradish peroxidase (HRP). As the HRP concentration increases, the system exhibits a ratiometric change, with the fluorescence intensity at 490 nm continuously increasing and the fluorescence intensity at 560 nm continuously decreasing. Therefore, quantitative and visual detection of HRP can be achieved, with good selectivity, strong anti-interference ability, and high sensitivity.

A horseradish peroxidase hydrogel photonic crystal sensor and method, by double-bonding horseradish peroxidase HRP as a recognition molecule, enabling the double-bonded horseradish peroxidase HRP to form a polymer hydrogel by cross-linking with other monomers acrylamide AAm having double bonds to embed colloidal particles in a photonic crystal array, so that the specific binding of horseradish peroxidase HRP and hydrogen peroxide can be used to realize sensitive label-free visual detection of hydrogen peroxide content in the measured object by changes in gel volume (colloidal particle spacing) and changes in the Debye ring diameter diffracted by the photonic crystal array caused by the colloidal particle spacing.

The invention discloses a horseradish peroxidase mutant with improved thermal stability and construction and application of genetically engineered bacteria of the horseradish peroxidase mutant, and belongs to the technical field of biology. The horse radish peroxidase mutant capable of efficiently catalyzing oxidation reaction of various organic and inorganic substrates is constructed, compared with wild type HRP, the half-life period of the mutant at the temperature of 50 DEG C is prolonged, and the half-life period of double mutants can be prolonged to 4 times that of the wild type HRP. The horse radish peroxidase variant disclosed by the invention has a wide application prospect, particularly shows excellent catalytic efficiency and stability under harsh reaction conditions, and provides a better enzyme tool for the fields of industry and biomedicine.

The present disclosure relates generally to compositions and methods for generating hydrogel coatings on features. In some embodiments, hydrogel-coated features are prepared by horseradish peroxidase-mediated gelation of microspheres. Some embodiments relate to methods for generating hydrogel coatings using particle templated emulsification. The disclosure also relates to the use of hydrogel-coated features in arrays for spatial analysis of biological analytes in biological samples.

The application provides a preparation method of a nanocolloid composition, a glucose detection method and a glucose biosensor, and relates to the technical field of glucose detection.The preparation method of the nanocolloid composition comprises the following steps: performing free radical polymerization reaction on glucose oxidase, horseradish peroxidase, a water-soluble monofunctional polymerizable monomer and a water-soluble multifunctional polymerizable monomer.The nanocolloid composition is suitable for detecting glucose in a body fluid, and has the characteristics of high detection sensitivity, high precision, high accuracy and the like.