464 results about "Horse-radish" patented technology

The invention discloses a chemiluminescence reagent, and relates to a chemiluminescence reagent which is mainly applied to a biotechnology, bioscience, medical diagnosis, food safety, and environment-friendly detection. The chemiluminescence reagent is prepared from the materials by mass percent: at least one 0.001-0.1% luminous reagent, at least one 0.001-0.2% oxidant, at least one 0.01-0.5% luminous enhancer, at least one 0.000001-0.05% background control agent, and a buffer solution of which the pH value is 8-11. The key is as follows: chemiluminescence is adjusted by utilizing the background control agent (particularly the antioxidant); the chemiluminescence reagent is valid on background control of enhanced chemiluminescence enzyme immunoassay detection, improvement of the signal to noise ratio and the like. The basis is how to control luminol free radical and concentration of related free radical in a horse radish peroxidase-luminol luminescence system.

The invention discloses a method for quantitative detection of biological molecules capable of being metabolized to generate H2O2 in serum by means of a ratiometric fluorescent probe. The method includes firstly preparing copper-nitrogen codoped carbon dots Cu-CDs; then reacting corresponding oxidase and the biological molecules capable of being metabolized to generate H2O2 to generate H2O2; catalyzing the H2O2 and a substrate that is o-phenylenediamine with horseradish peroxidase to generate an oxidation product DAP having yellow fluorescence; then adding the Cu-CDs into the DAP; allowing the DAP and the Cu-CDs to form ratiometric fluorescence, with the ratio I<572>/I<460> of fluorescence intensities of the DAP and the Cu-CDs being in a linear relationship with the concentration of a substance to be detected; and performing quantitative assay of the biological molecules in the serum according to the ratio of fluorescence intensities. The method is high in sensitivity and simple and convenient in detection, and has high selectivity and high affinity of immunoreactions.

The invention discloses a toxoplasma gondii IgM antibody detection kit combined with the FITC-anti-FITC indirect coating technology and the chemiluminescent immunoassay technology, and a preparation method thereof. The kit of the invention is composed of a negative control, a positive control, solid-phase vectors for anti-FITC antibodies, anti-human Mu-chain monoclonal antibodies of FITC markers, toxoplasma gondii antigens which are marked by horse radish peroxidase, chemiluminescent substrates and concentrated washing solutions. The kit of the invention can be used as the aided detection index for prenatal prepotency diagnosis, and has vital significances for improving the birth population quality and doing the family planning and the prepotency well.

The invention relates to a diagnostic reagent kit for testing the hepatitis c virus (HCV) and the preparation and test method, which is to add the HCV recombinant antigen used for peridium into the buffer solution, blend it, move into the luminous microplate, make incubation for 18 hours under 4DEG.C, wash the luminous microplate, add into the confining liquid, leave the liquid after incubation and fully dry the luminous microplate to complete the preparation of the pre-peridium luminous microplate; combine the anti-human IgG used for marking and the horse radish peroxidase by improving the sodium periodate to complete the preparation of the enzyme marker; prepare the chemical luminous substrate solution A with luminal, Tween20 and luminous intensifier and prepare the chemical luminous substrate solution B with the hydrogen peroxide. The reagent kit also comprises the sample diluent and concentrated scrub solution. The negative corresponds to the normal human serum while the positive corresponds to the people with serum of pooled serum with HCV antibody. The reagent kit provided in the invention has much higher detection sensitivity than the ELISA, which is safe and reliable, easy to operate with low cost, and without any expensive full-automatic chemical luminous measuring apparatus required.

The invention belongs to the field of detecting a trace amount of ions in the water environment, and particularly relates to a visualization method for rapidly detecting a trace amount of uranyl ions in the water environment. The method mainly includes the steps that DNAzyme with the specific recognition function on UO2 <2+> is fixed to the surfaces of magnetic beads, and horse radish peroxidase is preassembled on the surface of nano-gold; then the magnetic beads are connected with the nano-gold through the cutting effect of the UO2<2+> on the DNAzyme and the hybridization reaction of DNA, after separation and collection are carried out through an external magnetic field, H2O2 oxidation tetramethyl benzidine is efficiently catalyzed through the horse radish peroxidase to enable a solution to be changed from the blank to the blue, and therefore sensitive and specific visualization rapid detection of the UO2<2+> ions is achieved. As the method has the advantages of being high in sensitivity, high in specificity, high in matrix interference resistance, simple, rapid, low in cost and the like, the method can be used for site rapid visualization detection of the trace amount of UO2<2+> ions in various water samples.

The invention discloses an on-site detection immuno-chip, comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other; the double antibody sandwich principle is adopted to detect the antigen to carry out the dot matrix of corresponding capture antibody, positive control and negative control on the solid phase carrier simultaneously; the antibody protein is connected with the solid phase carrier through the covalent bond and physical adsorption; the sample liquid to be detected and the chip are directly incubated; the antigen to be detected in the samples combine with the corresponding antibody which is fixed on the chip; a specific monoclonal antibody probe marked by horse radish peroxidase is added and the macroscopic detection results can be obtained after the coloration of substrates. The invention can detect viruses in multiple aquatic animal samples and the results are macroscopic, thus being applicable to rapid and accurate detection of viruses of aquatic animals in breeding production.

The invention discloses instant lipid-lowering glutinous rice flour and a processing method thereof. The instant lipid-lowering glutinous rice flour is prepared from following raw materials including, by weight, 300-350 parts of glutinous rice, 20-40 parts of gleditsia sinensis seeds, 30-50 parts of barley seedling powder, 15-20 parts of horse radish tree leaves, 60-80 parts of tartary buckwheat, 4-6 parts of camellia nitidissima, 2-3 parts of pueraria lobata stems, 2-3 parts of male flower of eucommia ulmoides, 2-3 parts of lotus leaves, 2-4 parts of siraitia grosvenorii flowers, 2-3 parts of rose fruits, 10-15 parts of mulberry leaf micro-powder, a proper amount of pomegranate wine, 8-15 parts of peony seed oil and 8-10 parts of food additives. The instant lipid-lowering glutinous rice flour is rich and balanced in nutrition, is mellow and sweet in mouthfeel and is rich in dietary fiber components. By means of a synergetic effect with various traditional Chinese medicine beneficial components, the instant lipid-lowering glutinous rice flour can accelerate metabolism, can reduce blood glucose and blood fat, is especially suitable for patients suffered from hyperlipidemia or diabetes, and has a body reinforcing and health caring effect when being eaten often.

The invention relates to magnetic graphene oxide composite material immobilized horse radish peroxidase as well as a preparation method and application thereof, belonging to the technical field of inorganic materials and analysis. The preparation method comprises the steps of firstly preparing a magnetic polymer microsphere containing 6-arm polyethylene glycol amide (6-arm-PEG-NH2) from magnetic graphene oxide GO-Fe3O4 and 6-arm-PEG-NH2, immobilizing horse radish peroxidase, and degrading pollutant phenol by virtue of immobilized horse radish peroxidase. The synthetic process of the material is reasonable in design, and the carrier material is coupled with zymoid catalytic activity of graphene oxide and high density amino functional groups on the surface of a multi-arm polymer 6-arm-PEG-NH2, so that the activity and reutilization capacity of immobilized horse radish peroxidase are effectively improved; furthermore, the degradation velocity of prepared immobilized horse radish peroxidase is obviously higher than the degradation velocities of free enzyme and a carrier material.

The invention discloses a kit capable of quantitatively detecting soluble B7-H1, which comprises a horseradish peroxidase label, tetramethylbenzidine serving as a reaction substrate, bovine serum albumin, an elisa plate, washing liquor, stop solution, a coating antibody, a standard protein and a detection antibody, wherein the coating antibody is a mouse anti-human B7-H1 monoclonal antibody and the nucleotide sequence of the heavy chain of the coating antibody is represented by a nucleotide sequence SEQ ID No.3 in the sequence list, and the nucleotide sequence of the light chain of the coating antibody is represented by a nucleotide sequence SEQ ID No.4 in the sequence table. The kit capable of quantitatively detecting soluble B7-H1 has high specificity and can be used for accurately quantitative analysis on soluble B7-H1 protein factor concentration in liquid for human cell culture supernate, serum, plasma, hydrothorax and the like.

The invention relates to a preparation method of magnetic chitosan microsphere immobilized horse radish peroxidases. The preparation method is used in the fields of enzyme engineering development application and environmental protection, and enzyme can be recycled after enzyme immobilization is carried out. The technical scheme is dissolving polyethylene glycol 4000in water, adding Fe<2+> salt and Fe<3+> salt according to the mass ratio of 1:4, dropwise adding stronger ammonia water until pH of a system is larger than or equal to 10, adjusting the pH to 7.0 by using acetic acid after 2h, and conducting magnetic field separation and washing to obtain magnetofluid; then preparing the acetic acid solution of chitosan magnetofluid according to mass ratio of the chitosan to the magnetofluid of3:1, ultrasonically blending, adding in atoleine containing emulsifier span 80 for emulsifying, adding glutaraldehyde for solidification for 3h, and conducting separation, washing and drying to obtain magnetic chitosan microspheres; and finally adding the magnetic chitosan microspheres in horseradish peroxide enzyme phosphate buffer, solidifying for 3h at 4DEG C, conducting separation, washing and filtering to prepare the magnetic chitosan microsphere immobilized horse radish peroxidases. Carriers of the method are cheap and easy to obtain, enzyme stability is good, and the preparation methodis favorable for rapid and repeated use of the enzyme.

The invention relates to a biosensor, particularly relates to a preparation method and catalytic application of a Nafion/horseradish peroxidase/tricobalt tetraoxide-graphene/ionic liquid carbon paste electrode (Nafion/HRP/Co3O4-GR/CILE) and belongs to the technical field of electrochemical analysis and detection. According to the preparation method of the Nafion/HRP/Co3O4-GR/CILE, an ionic liquid carbon paste electrode is used as a substrate electrode, and by virtue of the specific advantages of a Co3O4-GR nano composite material of large surface area and good biocompatibility, HRP is adsorbed on the surface of the material; and furthermore, the material is fixed by a Nafion film to prepare the Nafion/HRP/Co3O4-GR/CILE. HRP in the modified electrode keeps a natural structure and biological activity. Research on electrochemical properties of the modified electrode shows that the modified electrode has good electrochemical catalysis capability on trichloroacetic acid (TCA), and can be used for effectively detecting TCA, and the detection limit is 0.33mmol/L. The preparation method and the catalytic application, provided by the invention, do not cause environmental pollution; the modified electrode has good stability, repeatability and sensitivity, and the preparation process is simple, low in cost and easy to operate, so that the modified electrode has wide social benefits.

An enzyme electric couple catalyzing method for treating waste water containing phenol, aromatic amine and azo-dye is carried out by fixing horse-radish peroxidase or analog enzyme with enzyme activity on grinded pyrolyzed graphite electrode surface by surface activator and carboxy-carbon nano-pipe, making into solid-carried enzyme or analog enzyme electrode, taking solid-carried enzyme or analog enzyme electrode as cathode, taking graphite electrode as anode, pumping waste water containing phenol, aromatic amine and azo-dye into cathode chamber, inducing oxygen and air into cathode chamber to saturate, controlling cathode electric potential at -1.00--0.500V vs.SCE by constant potentiometer and constant-potential electrolyzing. It has fast degradation speed and high current efficiency.

The invention discloses a double-stained kit for auxiliary diagnosis of benign or malignant hepatocellular tumor and application thereof. The kit comprises the following components: mixed primary antibody of Arginase-1 and Glypian-3, mixed second antibody of alkaline phosphatase conjugated goat anti-rabbit second antibody and horse radish peroxidase labeled goat anti -mouse second antibody, chromogenic reagent AP-Red and DAB (Diaminobenzidine), and TBS (Tris buffer saline) buffer solution. Two immunohistochemical labels provided by the invention are used for auxiliary diagnosis of benign or malignant hepatocellular tumor and differentiation degrees of tumor, the two labels are displayed through staining label by different detection systems and utilizing the differences of target cells stained by each label, information quantity and differential staining on the same radiograph are improved, readability and accuracy of radiograph reading are increased, so that the double-stained kit has important significance to identification of benign or malignant hepatocellular tumor and differentiation degrees of tumor.

The invention, which belongs to the technical fields of immunology and food safety analysis, relates to an enzyme-linked immunosorbent assay kit for detecting skeletal muscle troponin I of cattle or sheep, and a preparation method thereof and application thereof. The kit comprises an enzyme label plate coated with a capture antibody, a horseradish-peroxidase-labeled detection antibody, a skeletalmuscle troponin I standard solution of cattle or sheep, a substrate coloured solution, a stop solution, and a concentrated washing solution. The capture antibody is obtained by secretion of a hybridoma cell line 3A8 with the preservation number of CCTCC NO:C2018217; and the detection antibody is obtained by secretion of a hybridoma cell line 3D3 with the preservation number of CCTCC NO:C2018218. The kit has advantages of high sensitivity, high precision, high accuracy, and low cross-reaction rate and is suitable for large-scale sample detection.

The invention discloses a polymer-coated aramid fiber and a preparation method thereof. The preparation method comprises the following steps: 1, mixing an inorganic salt with an inorganic alkali solution, and diluting with water to prepare a buffer solution; 2, placing an aramid fiber in the buffer solution under the protect of nitrogen, adding a solvent, horseradish peroxidase and an acrylic acid monomer, adding hydrogen peroxide drop by drop, and carrying out washing and vacuum drying after finishing a reaction to obtain the polymer-coated aramid fiber. By adopting above technical scheme of the invention, the enzyme inactivation rate is low, and the surface of the aramid fiber has a good swelling effect in a reaction medium, so the full contact of the biological enzyme and the fiber is guaranteed, and the grafting rate is improved; and the formed coating which contains active groups allows the cohesiveness of the fiber with other materials to be effectively improved, so a large space is provided for the further modification of the fiber. The technical scheme adopted in the invention has the characteristics of low damage to the fiber, mild reaction condition, simple and easily implemented technology, environmental protection, and high benefit of the industrialization production.

The invention provides a thyrotropin (TSH) chemiluminescent immunoassay quantitative detection kit and a preparation method thereof. The invention has the advantages that the reaction pattern of the double antibody sandwich method is adopted, the chemiluminescent technology combining with the technology of fluorescin isothiocyanate-anti-fluorescein isothiocyanate is effectively utilized, horse radish peroxidase is adopted to be as the marker enzyme to quantitatively detect the content of TSH in the human serum sample, the detection sensitivity is guaranteed, and the raw materials are greatly saved. The kit of the invention has high sensitivity, good repeatability, short reaction time, good stability and long reagent validity, and can provide the experimental basis timely for the clinical diagnosis and the treatment of thyroid disorders.

The invention discloses an indirect ELISA kit for detecting African swine fever virus (ASFV) antibody, and a use thereof, and belongs to the technical field of biology. The kit is characterized by: adopting prokaryotic expressed recombined P54 protein as coating antigen; detecting antibody against to the ASFV in swine serum according to an indirect ELISA principle. The coating antigen in 96-well plates of the kit is the prokaryotic expressed recombined P54 protein which has good antigenicity. The enzyme-linked immunospecific assay kit provided by the present invention comprises the 96-well plates coated by the P54 protein, positive control, negative control, horseradish peroxidase-labeled rabbit anti-porcine IgG polyclonal antibody, a concentrated cleaning solution, a serum dilution, a TMB substrate indicator and a stop buffer. The kit provided by the present invention can be provided for screening mass samples. In addition, the main reagents in the kit are provided in a working fluid manner such that the reagents are used conveniently.

The invention provides a CsxWO3 type peroxide mimic enzyme, and a preparation method and application thereof. The CsxWO3 type peroxide mimic enzyme has the activity similar to that of peroxidase, and can be used for catalyzing H2O2 to generate a great quantity of hydroxyl free radicals and effectively oxidizing a substrate, 3,3',5,5'-tetramethyl benzidine to generate a color developing reaction. The CsxWO3 type peroxide mimic enzyme is combined with glucose oxidase for use, the content of glucose in a solution can be indirectly detected by measuring an ultraviolet absorption value of the oxidized 3,3',5,5'-tetramethyl benzidine and the detection limit is 0.1 micro milliliter. Compared with horse radish peroxidase (HRP), the CsxWO3 nano material has the advantages of simple preparation process, low cost, stable chemical activity and the like; the catalytic effect and the applicability of the CsxWO3 nano material are higher than those of natural peroxidase, and thus the CsxWO3 nano material can be used as a substitute of natural peroxidase.

The invention discloses a detection kit and a detection method of mercury ions. The kit includes: gold nanoparticles which are used for combining with Hg<2+> to form an horseradish peroxide mimetic enzyme; a single-chain nucleic acid which is used for combining with the gold nanoparticles to enhance a catalytic activity of the horseradish peroxide mimetic enzyme; a characteristic substrate of the horseradish peroxide mimetic enzyme; and auxiliary reagents including a sodium citrate buffer solution which is required when an oxidized characteristic substrate is catalyzed by the horseradish peroxide mimetic enzyme. The detection method is carried out mainly on the basis of the detection kit. By means of the kit and the method, high-sensitivity detection of Hg<2+> can be achieved with a linear range of the detection of the Hg<2+> being 10-1000 nM and a sensitivity being 3.0 nM. The kit and the method are simple, convenient and quick, are low in cost, are high in stability and are suitable for being used in detection of the Hg<2+> in samples of environment, food and the like.

The invention relates to a method for testing hydrogen peroxide in a cell based on a horseradish peroxidase-attapulgite nanometer composite material; the method comprises the following steps: horseradish peroxidase is adsorbed on the surface of purified attapulgite so as to prepare the horseradish peroxidase-attapulgite nanometer composite material; a dispensing method is used for applying the composite material to the surface of a glassy carbon electrode so as to construct a horseradish peroxidase-attapulgite nanometer composite material biological sensor; the sensor is used as a working electrode; and a chronoamperometry is used for detecting H2O2 in the cell. The biological sensor adopted by the method for testing the hydrogen peroxide in the cell has good electrical catalytic activity on hydrogen peroxide, can be applied to the detection of H2O2 in the cell and has the advantages of fast response, wide linear range, low detection limit, good repeatability and the like; and a new electrochemistry method based on enzyme sensing for detecting H2O2 in the cell is established.

The invention provides a detecting method and an application of the extraneous activity of the RNA polymerase relied on by the hepatitis C virus RNA, which relates to an secure and practical establishing method for the detection of the activity extraneous detection of the RNA polymerase relied on by the hepatitis C virus RNA, and the application relates to the genetic engineering technology and the nanometer segregation enrichment and RNA related technology field. The invention expresses the soluble NS5B protein through the utilization of the genetic engineering technology, and prepares the nanometer magnetic particle. One end of a RNA template is fixed on the nanometer magnetic particle, and added in the 96 hole reaction plate micromesh, and added with NS5B protein to compose a RNA duplication system, and a duplicated RNA double chain on the nanometer magnetic particle is mixed with marked biological elements. After the RNA is duplicated, the nanometer magnetic particle is attached at the micromesh bottom through an external magnetic field magnet, after repeatedly cleaning and attaching, the nanometer magnetic particle is combined with the marked combining factor of the horse radish peroxidase, after attaching and cleaning, the substrate constant of the horse radish peroxidase is added to develop color. New NS5B protease activity inhibitor can be screened from a traditional Chinese storeroom and a compound storeroom by the established method.