94 results about "Horse-radish" patented technology

The invention relates to a bioelectrochemical sensor used for detecting hydrogen peroxide and a manufacturing method of the bioelectrochemical sensor. The novel bioelectrochemical sensor is a sensor with a three-electrode system, wherein the counter electrode is a platinum electrode, the reference electrode is saturated calomel electrode and the working electrode is gold electrode. The sensor is based on orientation combination of a polypeptide horse radish peroxidase with characteristic sequence, the promotion effect of the polypeptide horse radish peroxidase on the activity of the enzyme and the characteristic of high sensitivity of electrochemical detection methods. The sensitivity of hydrogen peroxide sensors is effectively improved and satisfactory results are obtained.

The invention discloses a lasting aromatic lyocell fiber of a biological enzyme immobilized temperature-sensitive microcapsule and a preparation method of the lasting aromatic lyocell fiber, and relates to the technical field of polyester fabrics. The method comprises the steps that firstly, a temperature-sensitive microcapsule of a three-layer structure is prepared, an inner core contains aromatic substances of lavender essential oil, ambrota ether and rosin glycerin ester, a middle layer enables N-isopropylacrylamide monomers to be polymerized to form PNIPAM temperature-sensitive hydrogel, and a shell is a nanocellulose crystal-polydopamine compound; then spinning by taking the NMMO aqueous solution containing the microcapsules as an inner layer solution and taking the NMMO aqueous solution containing the eucalyptus pulp as an outer layer solution by adopting a coaxial micro-fluidic spinning technology; and finally carrying out crosslinking treatment through a biological enzyme coagulating bath, wherein the coagulating bath contains an NMMO aqueous solution, horse radish peroxidase and a hydrogen peroxide solution with gradient concentration. The aromatic lyocell fiber prepared by the invention has excellent temperature-sensitive aroma release performance and washing fastness, and solves the technical problems that microcapsules in traditional aromatic textiles are easy to break and fall off and aromatic substances are easy to volatilize.

The invention belongs to the technical field of biomedical materials and tissue regeneration, and particularly discloses hydrogel with a self-enhanced piezoelectric effect as well as a preparation method and application of the hydrogel. The hydrogel is prepared by taking microphone (MXene), cellulose nanofiber (CNF), tyramine grafted carboxymethyl cellulose (T-CMC) and regenerated silk fibroin (RSF) as main materials through an enzyme crosslinking reaction of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2). The hydrogel has excellent mechanical properties, biocompatibility and safety, and can realize self-enhancement in vivo and generate stable piezoelectric signals. The piezoelectric stimulation can promote proliferation, migration and differentiation of osteogenesis-related cells and regulate and control a bone tissue microenvironment, so that formation and repair of new bones in a bone defect area are remarkably accelerated. The hydrogel can be used as an injectable or moldable bone tissue repair material, and is suitable for bone fracture, bone defect and other bone tissue regeneration scenes.

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention discloses an antibacterial dressing for promoting wound repair and regeneration and a preparation method thereof, and the preparation method comprises the following steps: adding hydrogen peroxide into a silk fibroin aqueous solution to prepare a silk fibroin aqueous solution premixed with hydrogen peroxide; horse radish peroxidase is added into the dopamine modified sodium alginate solution, and the dopamine modified sodium alginate solution premixed with horse radish peroxidase is prepared. Preparing a chondroitin sulfate composite solution; uniformly mixing the silk fibroin aqueous solution premixed with hydrogen peroxide, the dopamine modified sodium alginate solution premixed with horse radish peroxidase and the chondroitin sulfate composite solution. Hydrogel formed by cross-linking silk fibroin and chondroitin sulfate is used as a main body material of the dressing, and meanwhile, dopamine modified sodium alginate is introduced, so that the dressing has the photo-thermal antibacterial characteristic; magnesium oxide microspheres are introduced as a pore-foaming agent, micropores are formed in the dressing, and the liquid absorption property and the air permeability of the dressing are improved; the umbilical cord mesenchymal stem cell exosome is introduced, so that angiogenesis at the wound is promoted, and the wound healing promoting capability of the dressing is improved.

The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.

The invention discloses a preparation method of a stable Pickering emulsion based on enzymic method modified whey protein microgel particle-araboxylan covalent composite particle, which comprises the following steps: preparing whey protein microgel particles by cross-linking glutamine transaminase, mixing the whey protein microgel particles with an araboxylan solution, and adding an enzyme method modified whey protein microgel particle-araboxylan covalent composite particle to prepare the Pickering emulsion. Adding horse radish peroxidase and a hydrogen peroxide solution to carry out enzymatic modification to obtain a water phase, mixing the oil phase and the water phase according to a volume ratio, carrying out IKA homogenization, and then carrying out ultrasonic treatment to obtain a Pickering emulsion with stable covalent composite particles; the preparation method is simple, harmful substances cannot be generated in the preparation process, and meanwhile, the preparation method is low in production cost, short in production period, rich in nutrition, green, free of pollution and high in economic utilization value; the obtained Pickering emulsion has high viscosity and excellent storage stability and environmental stability, an effective delivery carrier construction strategy is provided for construction of a fat-soluble and heat-sensitive bioactive micromolecule delivery system, and the application range of protein microgel particles in the fields of food, medicine and the like is widened.

The invention provides a drug-loaded sophorolipid microsphere, a preparation method thereof and application of the drug-loaded sophorolipid microsphere in preparation of a bionic cartilage scaffold. The preparation method of the drug-loaded sophorolipid microspheres comprises the following steps: (1) preparing a drug-containing sophorolipid solution; (2) preparing multiple emulsion; and (3) adding a horse radish peroxidase solution into the multiple emulsion for reaction to obtain the drug-loaded sophorolipid microspheres. The bionic cartilage scaffold sequentially comprises a bone layer, a transition layer and a cartilage layer from bottom to top, the bone layer is a cross-linked compound of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the transition layer is a physical mixture of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the cartilage layer is drug-loaded sophorolipid microspheres. The drug-loaded sophorolipid microspheres disclosed by the invention have good biocompatibility, excellent biocompatibility and good mechanical properties. The bionic cartilage scaffold utilizes the characteristics of the nHA and the drug-loaded sophorolipid microspheres to form a unique network structure, so that material failure caused by mechanical mismatching is avoided.

Nucleotide compositions, recombinant vectors, and recombinant viral genomes are disclosed that include a modified horseradish peroxidase (HRP) gene sequence, as well as recombinant viruses that have the modified HRP gene sequence incorporated into the viral genome thereof. The recombinant viruses are utilized in an HRP-based assay to analyze the neutralization potential of candidate antiviral agents, such as antiviral antibodies.

The application provides a glucose detection test strip and a preparation method and application thereof. The preparation method of the glucose detection test strip comprises the following steps: taking copper sulfate pentahydrate, a phosphate buffer solution, glucose oxidase and horseradish peroxidase, mixing uniformly to obtain an enzyme-containing mixed solution; immersing a test strip in the enzyme-containing mixed solution, and obtaining an organic-inorganic hybrid nanoflower loaded test strip after reaction; immersing the organic-inorganic hybrid nanoflower loaded test strip in a mixed solution containing a chromogenic agent, a gel, bovine serum albumin and polyethylene glycol to obtain a glucose detection test strip. The preparation method of the glucose detection test strip has higher sensitivity and shorter response time.

The invention discloses an up-conversion nano-composite with a core-shell structure, a preparation method and application, and belongs to the technical field of biomedical bimodal nano-probes. According to the nanocomposite, up-conversion nanoparticles (UCNPs) serve as a core, metal-organic frameworks (MOFs) serve as a shell layer, horse radish peroxidase (HRP) is loaded on the shell layer, and UCNP (at) MOF / HRP is formed. Subsequent biological functional modification, such as coupling of an antibody, an antigen, nucleic acid and other substances, can be carried by an activating group or a metal coordination bond carried by a shell ligand of the upconversion nanocomposite MOF with the core-shell structure, so that the functions of specific recognition, targeted capture, specific loading and the like are realized.

The invention discloses a chemiluminescence immunoassay method and kit for detecting progestational hormone of pandas, and belongs to the technical field of biological detection. The specific process comprises the following steps: by taking goat anti-mouse IgG as a coating antibody, enabling a progestational hormone standard substance or a to-be-detected sample and horse radish peroxidase labeled progestational hormone to compete with a limited amount of progestational hormone antibodies together; after incubation and washing, a chemiluminescent substrate is added, and quantitative analysis of the progestational hormone in the panda urine or excrement is realized by detecting the intensity of a luminescent signal according to a standard curve. The invention provides a novel chemiluminescence immunoassay method, the sensitivity of the chemiluminescence immunoassay method is far higher than that of a traditional ELISA method (the lowest detection line can reach 31.25 pg / mL), the operation is simple and convenient, the detection is rapid, and the urgent demand on rapid and accurate determination of the hormone level in panda pregnancy monitoring and reproduction management can be met.

The invention provides a rapid detection kit and a rapid detection method for phytosterol, and belongs to the field of food detection.The rapid detection kit for phytosterol comprises a phytosterol standard substance, a standard substance diluent, a substrate solution, an enzyme mixed solution, a stop solution and an elisa plate; the standard substance diluent comprises isopropanol or dimethyl sulfoxide; the substrate solution is prepared from TMB (Tetramethylbenzidine) and a substrate buffer solution; the enzyme mixed solution is a mixed solution of a cholesterol oxidase solution and a horse radish peroxidase solution. By optimizing the composition of each working reagent in the kit, the sensitivity and accuracy of phytosterol detection are remarkably improved. Meanwhile, the rapid detection method provided by the invention is simple and convenient to operate, short in detection time and suitable for on-site rapid detection.

The application discloses a high-stability horseradish peroxidase, a recombinant expression vector, a preparation method and application. The amino acid sequence of the horseradish peroxidase is shown as SEQ ID NO. 1. Compared with wild-type horseradish peroxidase, the enzyme activity of the horseradish peroxidase is increased by 3.2 times, and compared with commercially available horseradish peroxidase, the thermal stability is better. The method can produce the horseradish peroxidase by using a tobacco transient expression system. Compared with an Escherichia coli expression system and a yeast expression system, the tobacco transient expression system can correctly fold and glycosylate, and has higher enzyme activity and stability. The purity of the horseradish peroxidase obtained according to the method can reach RZ 3.2, the enzyme activity can reach 275 U / mg, and the horseradish peroxidase can be applied to immunological detection.

The application relates to the field of biotechnology, and in particular to a wheat allergen specific IgE detection kit and a detection method thereof. The kit comprises magnetic particles, enzyme-labeled anti-human IgE antibodies and biotin-labeled wheat allergen. The wheat allergen is obtained by treating a wheat protein extract with heat treatment or a pretreatment agent. The pretreatment agent comprises one or more of sodium azide, L-cysteine and L-cysteine hydrochloride. The wheat allergen is biotin-labeled after specific pretreatment. The chemiluminescence immunoassay method uses streptavidin to prepare magnetic microspheres and horseradish peroxidase-labeled anti-human IgE antibodies. The method is fully automatic, ensures the accuracy of the detection result, and improves the test flux, the specificity and the sensitivity of the detection result.

The invention provides photo-thermal response enhanced hydrogel loaded with black rice extract as well as a preparation method and application of the photo-thermal response enhanced hydrogel loaded with the black rice extract, and the photo-thermal response enhanced hydrogel is formed by taking a mixture of hyaluronic acid / tyrosine conjugate and the black rice extract as a base material under the cross-linking action of horse radish peroxidase / catalase, horseradish peroxidase acts on the black rice extract while serving as a cross-linking agent, the near-infrared photo-thermal responsiveness of the black rice extract after reaction of the horseradish peroxidase is greatly enhanced, and good oxidation resistance, inflammation resistance and photo-thermal responsiveness can be achieved only through a small amount of black rice extract in a hydrogel system.

The application discloses a urine tyrosine detection system, a detection method and a detection kit. The detection system comprises a buffer solution, 4-aminoantipyrine, horseradish peroxidase and hydrogen peroxide, and the pH of the detection system is 6.5-7.8. After the sample to be detected is placed in the detection system and reacts at room temperature, the quantitative analysis of tyrosine can be realized by observing the color change of the reaction solution or testing the absorbance value of the reaction solution at 485 nm. Compared with the traditional Millon reaction and the multi-enzyme reaction system, the reagent used in the application has no toxicity and corrosion, does not cause hidden troubles to human health and environmental safety, is simple to operate, does not need special instruments and has low cost, and is conducive to realizing 'home health detection'.

The application belongs to the field of material preparation, and particularly relates to a method for in-situ synthesis of microcapsules by enzyme catalysis and application thereof in anticorrosive coatings. The method specifically comprises the following steps: firstly, preparing a cellulose nanocrystal dispersion liquid and an emulsion of flaxseed oil containing cembrene; ultrasonic dispersing the emulsion, and then performing magnetic stirring to obtain a Pickering emulsion; adding hydrogen peroxide and horseradish peroxidase solution into the Pickering emulsion, and stirring and reacting; and performing freeze-drying on the emulsion after the reaction is completed to obtain cellulose nanocrystal microcapsules. The application uses an enzyme catalysis method to prepare microcapsules with an anticorrosive function, and the synthesized microcapsules do not contain harmful substances such as formaldehyde and are environmentally friendly. The synthesized microcapsules have good stability, can be easily dispersed in an aqueous solution, can be used as a carrier of healing reagents for water-based anticorrosive coatings, and have a simple preparation method, controllable size and suitability for large-scale production.

The invention belongs to the technical field of special medical food, and relates to application of common turnip in products for inhibiting gastrointestinal tract helicobacter pylori. The common turnip extract has the function of treating or preventing helicobacter pylori infection, has a good improvement effect on prevention and inhibition of gastroenteritis caused by helicobacter pylori, remarkably improves the function of a digestive system, and can be used for preventing and treating the gastroenteritis caused by the helicobacter pylori and preventing and treating the gastroenteritis caused by the helicobacter pylori. And support is provided for developing novel dietary nutrition supplements, functional foods, special medical foods and pharmaceutical compositions for inhibiting helicobacter pylori. The invention further discloses a synergistic effect of a medicinal and edible composition formed by combining the common turnip extract with extracts of clove, codonopsis pilosula, hericium erinaceus, fructus amomi and the like on inhibition of helicobacter pylori, and a greater effect of inhibiting the activity of the helicobacter pylori can be achieved. The novel product for inhibiting helicobacter pylori developed by the invention has both safety and effectiveness, and has a very good application prospect.

The invention relates to the technical field of PE pipes, in particular to an anti-aging high-toughness PE pipe and a preparation method thereof.The anti-aging high-toughness PE pipe is prepared from, by weight, 40-60 parts of high-density polyethylene, 20-30 parts of linear low-density polyethylene, 8-15 parts of an additive, 5-10 parts of a functional additive, 0.5-1.2 parts of calcium stearate and 0.3-0.8 part of a composite antioxidant B900. The functional additive is prepared by mixing tannic acid, nano silicon dioxide, neutral protease and horse radish peroxidase for reaction under an acidic condition and carrying out heating curing, centrifugal separation, drying and crushing processes, so that the problem that a common PE pipe is easy to age and embrittle in an outdoor environment is solved, the service life of the pipe is prolonged, and the service life of the pipe is prolonged. Active groups generated by enzymatic reaction in the functional auxiliary agent enhance the interfacial compatibility of the filler and a polyethylene matrix, so that long-term stable use of the filler in a complex environment is guaranteed.

The application relates to the technical field of materials, in particular to a monodisperse core-shell microsphere and a preparation method thereof. The method comprises the following steps: S1, using a dispersion phase liquid containing a degradable polymer, forming monodisperse degradable polymer microspheres through a microfluidic process or an emulsification method; S2, combining horseradish peroxidase through one or more of the following methods: dopamine self-polymerization, electrostatic adsorption and chemical grafting on the surface of the degradable polymer microspheres, and under the catalysis of the horseradish peroxidase, crosslinking and coating the degradable polymer microspheres with a hydrogel material to obtain monodisperse core-shell microspheres with a hydrogel layer; the hydrogel material is a hydrogel system that can be crosslinked by enzyme initiation, such as hydroxyphenylpropionic acid hydrogel, silk fibroin or hyaluronic acid hydrogel. Through the method, highly monodisperse degradable core-shell microsphere materials can be obtained, the process operation is simple, and the method is beneficial to wide popularization and application.

The invention provides a nano-enzyme system based on an Ag-polydopamine nano-microsphere (at) MOF-74 structure as well as a preparation method and application of the nano-enzyme system, and belongs to the technical field of biological medicines. The nano-enzyme system takes a polydopamine microsphere as a core, and an MOF-74 shell layer grows on the surface of the polydopamine microsphere in situ to form a composite microsphere with a core-shell structure; and by utilizing the reduction property of polydopamine, Ag nanoparticles are reduced in situ and uniformly dispersed in the MOF-74 framework, so that the composite nano material with the enzyme-like activity and the photo-thermal function is constructed. The system shows excellent horse radish peroxidase-like activity, and can effectively promote electron transfer between hydrogen peroxide and a substrate and catalyze a chromogenic reaction. Meanwhile, the composite system has good photo-thermal conversion performance, shows a remarkable photo-thermal sterilization effect on gram-negative bacteria and gram-positive bacteria, and can effectively inhibit and destroy the formation of bacterial biofilms. An in-vitro antibacterial experiment result shows that the MOF-74-based nano enzyme system has relatively high antibacterial activity.

The application discloses a silk protein-based hydrogel optical fiber and a preparation method and application thereof, and belongs to the technical field of hydrogel optical fibers.The silk protein-based hydrogel optical fiber with a chemical-physical double crosslinking structure is constructed through a synergistic strategy of enzymatic crosslinking and ethanol solution post-processing; firstly, a degumming treatment is performed by using a sodium carbonate solution to prepare a silk protein solution; then, a core liquid and a coating liquid are respectively prepared by using the silk protein solution, a horseradish peroxidase solution and an H2O2 solution; the core liquid is injected into a gel, and then, an ethanol solution is used to strengthen the mechanical property of the core liquid; finally, the coating liquid is coated, and the silk protein-based hydrogel optical fiber with excellent mechanical property, low optical loss and good biocompatibility is obtained.

The invention discloses a poly HRP-nano antibody conjugate as well as a preparation method and application thereof. The invention aims to provide a method for generating a plurality of active aldehyde groups on the surface of horse radish peroxidase through systematically optimizing key parameters such as reaction temperature, oxidation time and reactant molar ratio by utilizing a periodate oxidation method; then, the activated HRP reacts with a primary amine group on the nano antibody to form a high-molecular-weight functional polymer conjugate; according to the scheme, the advantage of small molecular weight of the nano antibody is utilized, and the limitation that the traditional IgG antibody is difficult to form a high-valence functional polymer due to a steric hindrance effect in coupling is overcome; the prepared conjugate can remarkably improve the detection sensitivity of immunoassay, the reaction yield is high, a crude product of the conjugate can be directly applied to detection without purification, the preparation process is greatly simplified, the application cost is reduced, and a simple, convenient, universal and efficient solution is provided for developing a novel high-sensitivity immunodetection reagent; belongs to the field of biotechnology and in-vitro diagnosis.

The application discloses a horseradish peroxidase-labeled antibody freeze-drying preparation and a preparation method thereof, relates to the technical field of biological detection, and the freeze-drying preparation comprises a horseradish peroxidase-labeled antibody, a protein protective agent and a buffer; the protein protective agent comprises the following preparation raw materials in parts by weight: 30-40 parts of trehalose, 0.5-2 parts of oxidized sodium alginate, 0.1-0.4 parts of 2, 4, 6-tris (4-aminophenyl) -1, 3, 5-triazine, 8-12 parts of betaine, 3-6 parts of proline, 0.5-0.7 parts of bovine serum albumin, 2-2.5 parts of glycine, 0.25-0.35 parts of beta-cyclodextrin, 0.5-0.7 parts of a reducing agent and 0.25-0.4 parts of a surfactant. The application has the effects of improving the storage stability and activity recovery rate of the horseradish peroxidase-labeled antibody.

The invention relates to the technical field of bioenergy active hydrogel, in particular to silk fibroin hydrogel with bioenergy activity and a preparation method of the silk fibroin hydrogel. The preparation method comprises the following steps: carrying out esterification reaction on a silk fibroin solution containing hydroxyl and phenolic hydroxyl and succinic anhydride to obtain succinic acid esterified silk fibroin; carrying out dissolution promoting treatment on the succinic acid esterified silk fibroin to obtain gel precursor liquid; a hydrogen peroxide solution of horse radish peroxidase and the gel precursor fluid are subjected to a cross-linking reaction, and the silk fibroin hydrogel containing succinic acid esterified silk fibroin is obtained. According to the preparation method, firstly, a silk fibroin solution and succinic anhydride are subjected to an esterification reaction, then horse radish peroxidase and a hydrogen peroxide solution are subjected to a cross-linking reaction, a stable hydrogel structure is formed, and the gel can improve the energy metabolism level of intervertebral disc cells.

The invention discloses a test strip for detecting Alzheimer's disease and application thereof, the test strip adopts a colloidal gold-horseradish peroxidase compound labeling technology, the detection sensitivity is remarkably improved through enzyme signal amplification, and two key AD biomarkers p-Tau-181 and A beta 1-42 in blood can be synchronously detected. The test strip comprises a sample pad, a marking pad, a nitrocellulose membrane and absorbent paper, wherein the nitrocellulose membrane is provided with two detection lines (T1 and T2) for capturing p-Tau-181 and A beta1-42 respectively and a quality control line (C). The kit is high in sensitivity, rapid in detection, simple and convenient to operate, low in cost and suitable for primary medical treatment and large-scale screening scenes, and has important value in early diagnosis, popularization, prevention and control of the Alzheimer's disease.

The invention discloses a wearable sensor-minimally invasive diabetes monitoring patch and a preparation method thereof, and belongs to the technical field of biomedicine, the patch comprises a microneedle array module and a sensing unit; the microneedle array module is composed of a plurality of microneedles arranged in an array; the sensing unit is loaded in the microneedle, and the sensing unit comprises a multi-shell metal organic framework cascade enzyme system and a chromogenic substrate; the multi-shell metal organic framework cascade enzyme system is of a double-layer structure composed of an inner core and an outer shell, the inner core is a metal organic framework packaged with glucose oxidase and modified with carboxyl functional groups, and the outer shell is a metal organic framework packaged with horse radish peroxidase. And 2, 2-nitrogen-bis (3-ethylbenzothiazoline-6-sulfonic acid) is taken as a chromogenic substrate. According to the patch, the catalytic efficiency and the detection accuracy are improved, the stability is improved, the storage and transportation cost is reduced, the low detection limit can be achieved, the requirement for instant blood glucose detection is met, the size is small, and the cost is low.

The application relates to the technical field of water treatment, in particular to a method for preparing a cationic tannin-based organic flocculant by an enzyme catalysis method, a product and application thereof. The method comprises the following steps: tannin and allyl triphenylphosphonium chloride are reacted under the action of an enzyme catalyst for 2-10 hours, the reaction temperature is 30-60 DEG C, the enzyme is inactivated after the reaction is completed, then the product is purified through acetone, cleaned through ethanol, dried in a vacuum drying box, and the cationic tannin-based organic flocculant is obtained; wherein the enzyme catalyst is lipase B or horseradish peroxidase. The application adopts an enzyme as a catalyst, realizes the covalent grafting between the phenolic hydroxyl group in the tannin molecule and the allyl group of allyl triphenylphosphonium chloride through the specific catalysis of the enzyme. The cationic tannin-based organic flocculant provided by the application can efficiently remove colloidal particles, heavy metal ions and dye pollutants in wastewater, and is especially suitable for treating high-turbidity and high-colority industrial wastewater.

The invention relates to a phage cocktail and application thereof, and belongs to the technical field of biology. The phage cocktail disclosed by the invention can be used for carrying out fluorescence labeling imaging and efficient killing on bacteria and infectious bacteria in a biofilm of the bacteria. A luminous photosensitizer, glucose oxidase and horse radish peroxidase are coupled to the surface of the phage to obtain three phage conjugates with different functions, and the three phage conjugates jointly form the phage cocktail. The three bacteriophage conjugates are simultaneously colonized in host bacteria by utilizing the targeting ability of the bacteriophages, and show efficient targeting, and the killing efficiency on the host bacteria can reach 100%. The phage cocktail can specifically recognize and fluorescently label bacteria in a biofilm, can efficiently destroy the structure of the biofilm and thoroughly eradicates the biofilm and antibiotic-sensitive and drug-resistant host bacteria in the biofilm.