224 results about "Horse-radish" patented technology

The invention relates to a bioelectrochemical sensor used for detecting hydrogen peroxide and a manufacturing method of the bioelectrochemical sensor. The novel bioelectrochemical sensor is a sensor with a three-electrode system, wherein the counter electrode is a platinum electrode, the reference electrode is saturated calomel electrode and the working electrode is gold electrode. The sensor is based on orientation combination of a polypeptide horse radish peroxidase with characteristic sequence, the promotion effect of the polypeptide horse radish peroxidase on the activity of the enzyme and the characteristic of high sensitivity of electrochemical detection methods. The sensitivity of hydrogen peroxide sensors is effectively improved and satisfactory results are obtained.

The invention discloses a washable easily-colored wool fabric treatment process, belongs to the technical field of wool fabric processing, and aims to solve the technical problem that the dyeing degree, the anti-felting performance and the color fastness of wool fabrics in the prior art need to be further improved. The method includes the following steps that disulfide bonds of wool fibers are opened through L-cysteine, the washable and easy-to-color wool fabric is obtained, the pretreated wool fibers prepared through the method are grafted with methoxy polyethylene glycol methacrylate under catalysis of hydrogen peroxide and horse radish peroxidase, modified wool fibers are obtained, and the washable and easy-to-color wool fabric is obtained. After the modified wool fibers are woven into the wool fabric, the wool fabric is respectively subjected to padding modification by using the modified dye solution and the finishing liquid, so that the degree of dyeing, the anti-felting performance and the color fastness of the wool fabric are improved.

The invention belongs to the technical field of biosensing instant detection, and relates to DAAO / HRP-MAF-7 hydrogel as well as a preparation method and application thereof. The hydrogel is formed by immobilizing horse radish peroxidase (HRP) and D-amino acid oxidase (DAAO) in a hydrophilic metal organic framework MAF-7, and then loading the prepared DAAO / HRP-MAF-7 biological composite material and a color developing agent TMB in PEG-DA hydrogel together. The DAAO / HRP-MAF-7 hydrogel can be used as a biosensor to be applied to detection of D-amino acid.

The invention discloses a dextran-based HRP-goat anti-mouse IgG labeled antibody and a preparation method thereof, and the preparation method is characterized by comprising the following steps: S1, oxidizing dextran to obtain aldehyde group-containing dextran; s2, carrying out polymerization on the dextran containing the aldehyde group and horse radish peroxidase, so as to obtain a horse radish peroxidase polymer; s3, performing desalination and ultrafiltration on the horse radish peroxidase polymer to obtain an ultrafiltration product I; s4, connecting a goat anti-mouse IgG antibody to the ultrafiltration product I to obtain oxidized glucan coupled with goat anti-mouse IgG and HRP enzyme; and S5, purifying the oxidized dextran of the product coupled with the goat anti-mouse IgG and the HRP enzyme, so as to obtain the dextran coupled HRP-goat anti-mouse IgG labeled antibody. The dextran product obtained by the invention is wide in molecular weight coverage, different molecular weights can be selected to design polymer substances with different sizes, the number of aldehyde groups can be controlled to control coupling of different numbers of protein molecules under the same molecular weight, and the signal intensity is easy to control.

The invention discloses a lasting aromatic lyocell fiber of a biological enzyme immobilized temperature-sensitive microcapsule and a preparation method of the lasting aromatic lyocell fiber, and relates to the technical field of polyester fabrics. The method comprises the steps that firstly, a temperature-sensitive microcapsule of a three-layer structure is prepared, an inner core contains aromatic substances of lavender essential oil, ambrota ether and rosin glycerin ester, a middle layer enables N-isopropylacrylamide monomers to be polymerized to form PNIPAM temperature-sensitive hydrogel, and a shell is a nanocellulose crystal-polydopamine compound; then spinning by taking the NMMO aqueous solution containing the microcapsules as an inner layer solution and taking the NMMO aqueous solution containing the eucalyptus pulp as an outer layer solution by adopting a coaxial micro-fluidic spinning technology; and finally carrying out crosslinking treatment through a biological enzyme coagulating bath, wherein the coagulating bath contains an NMMO aqueous solution, horse radish peroxidase and a hydrogen peroxide solution with gradient concentration. The aromatic lyocell fiber prepared by the invention has excellent temperature-sensitive aroma release performance and washing fastness, and solves the technical problems that microcapsules in traditional aromatic textiles are easy to break and fall off and aromatic substances are easy to volatilize.

The invention belongs to the technical field of biomedical materials and tissue regeneration, and particularly discloses hydrogel with a self-enhanced piezoelectric effect as well as a preparation method and application of the hydrogel. The hydrogel is prepared by taking microphone (MXene), cellulose nanofiber (CNF), tyramine grafted carboxymethyl cellulose (T-CMC) and regenerated silk fibroin (RSF) as main materials through an enzyme crosslinking reaction of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2). The hydrogel has excellent mechanical properties, biocompatibility and safety, and can realize self-enhancement in vivo and generate stable piezoelectric signals. The piezoelectric stimulation can promote proliferation, migration and differentiation of osteogenesis-related cells and regulate and control a bone tissue microenvironment, so that formation and repair of new bones in a bone defect area are remarkably accelerated. The hydrogel can be used as an injectable or moldable bone tissue repair material, and is suitable for bone fracture, bone defect and other bone tissue regeneration scenes.

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention discloses an antibacterial dressing for promoting wound repair and regeneration and a preparation method thereof, and the preparation method comprises the following steps: adding hydrogen peroxide into a silk fibroin aqueous solution to prepare a silk fibroin aqueous solution premixed with hydrogen peroxide; horse radish peroxidase is added into the dopamine modified sodium alginate solution, and the dopamine modified sodium alginate solution premixed with horse radish peroxidase is prepared. Preparing a chondroitin sulfate composite solution; uniformly mixing the silk fibroin aqueous solution premixed with hydrogen peroxide, the dopamine modified sodium alginate solution premixed with horse radish peroxidase and the chondroitin sulfate composite solution. Hydrogel formed by cross-linking silk fibroin and chondroitin sulfate is used as a main body material of the dressing, and meanwhile, dopamine modified sodium alginate is introduced, so that the dressing has the photo-thermal antibacterial characteristic; magnesium oxide microspheres are introduced as a pore-foaming agent, micropores are formed in the dressing, and the liquid absorption property and the air permeability of the dressing are improved; the umbilical cord mesenchymal stem cell exosome is introduced, so that angiogenesis at the wound is promoted, and the wound healing promoting capability of the dressing is improved.

The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.

The invention discloses a preparation method of a double-enzyme glassy carbon electrode, which comprises the following steps: placing a GCE electrode in a Piranha solution, cleaning the GCE electrode with distilled water, polishing the GCE electrode, and finally carrying out ultrasonic cleaning and drying. Carrying out activating treatment on the cleaned GCE electrode; the preparation method comprises the following steps: adding a mixture of tin dioxide and multi-walled carbon nanotube powder into a chitosan / acetic acid solution, and carrying out ultrasonic treatment, so as to obtain an MWCNTs-SnO2-CS nano compound; the preparation method comprises the following steps: modifying GCE by using a nano-composite to obtain GCE taking MWCNTs / SnO2 / CS as a modification material; choline oxidase and horse radish peroxidase are immobilized on GCE (Gelatin CE) which takes MWCNTs / SnO2 / CS as a modification material to prepare the double-enzyme GCE. The double-enzyme GCE prepared by the invention can be used for detecting the content of PC in crude oil, and a theoretical basis is provided for rapidly detecting the content of PC in crude oil.

The invention discloses a novel method for rapidly detecting ochratoxin A based on an electrochemical deposition polydopamine coating modified gold electrode, and belongs to the technical field of analytical chemistry and biology. The method is based on a polydopamine coating, is combined with an ochratoxin A aptamer with high specificity and high affinity, introduces a DNA-modified nanogold compound as a signal carrier and a signal amplification element, and realizes high-sensitivity detection of ochratoxin A through current change generated by catalyzing H2O2 / TMB by horse radish peroxidase captured on the surface of an electrode.

The invention discloses a preparation method of a stable Pickering emulsion based on enzymic method modified whey protein microgel particle-araboxylan covalent composite particle, which comprises the following steps: preparing whey protein microgel particles by cross-linking glutamine transaminase, mixing the whey protein microgel particles with an araboxylan solution, and adding an enzyme method modified whey protein microgel particle-araboxylan covalent composite particle to prepare the Pickering emulsion. Adding horse radish peroxidase and a hydrogen peroxide solution to carry out enzymatic modification to obtain a water phase, mixing the oil phase and the water phase according to a volume ratio, carrying out IKA homogenization, and then carrying out ultrasonic treatment to obtain a Pickering emulsion with stable covalent composite particles; the preparation method is simple, harmful substances cannot be generated in the preparation process, and meanwhile, the preparation method is low in production cost, short in production period, rich in nutrition, green, free of pollution and high in economic utilization value; the obtained Pickering emulsion has high viscosity and excellent storage stability and environmental stability, an effective delivery carrier construction strategy is provided for construction of a fat-soluble and heat-sensitive bioactive micromolecule delivery system, and the application range of protein microgel particles in the fields of food, medicine and the like is widened.

The invention relates to the technical field of biology, in particular to a method for stably improving the titer of horse radish peroxidase labeled streptavidin. According to the method provided by the invention, streptavidin with different histidine label lengths is designed, and then the streptavidin is coupled with horse radish peroxidase oxidized by sodium periodate, so that high-titer horse radish peroxidase labeled streptavidin can be obtained. The invention also discloses application of the enzyme-labeled conjugate in a magnetic particle platform streptavidin-biotin system, and experimental results prove that the method provided by the invention can be used for stably preparing the enzyme conjugate with high titer and low background, and is widely applied in the streptavidin-biotin system.

The invention discloses an ultrafast self-repairing hydrogel based on hyaluronic acid and gelatin and a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving hyaluronic acid in deionized water, adding EDC and NHS, stirring at room temperature, then adding a tyramine DMSO solution, reacting at room temperature, and dialyzing and freeze-drying after the reaction is finished to prepare a hyaluronic acid skeleton; (2) gelatin is dissolved in deionized water, a vanillin ethanol solution is added for a reaction, then a NaH3BCN solution is added for reduction, dialysis and freeze-drying, and a gelatin skeleton is prepared; (3) dissolving the hyaluronic acid skeleton in deionized water, and then adding horse radish peroxidase to prepare a hyaluronic acid solution; adding the gelatin skeleton solution into deionized water, and then adding hydrogen peroxide to prepare a gelatin solution; and (4) mixing the hyaluronic acid solution with the gelatin solution to prepare the composite hydrogel. The coating material is used for coating the probiotics and is beneficial to activity maintenance of the probiotics in a simulated digestive tract environment.

The invention relates to the technical field of chemiluminescence immunoassay, and discloses a horse radish peroxidase-labeled secondary antibody compound and a preparation method and application thereof, the horse radish peroxidase-labeled secondary antibody compound comprises a chitosan compound skeleton, and horse radish peroxidase and a secondary antibody carried on the chitosan compound skeleton; the chitosan compound skeleton is a macromolecular skeleton compounded by a chitosan spherical cross-linking body and dopamine. Through optimization and improvement of a polymer skeleton, the problems of large steric hindrance, low sensitivity and non-specific staining of a traditional polymer can be solved, and further improvement of the sensitivity of a DAB staining method for immunoassay detection is facilitated; the adopted chitosan compound skeleton has abundant amino groups, can bear more labeled enzymes and secondary antibodies, reduces steric hindrance, increases the penetrating power to a cell nuclear membrane, and improves the detection sensitivity.

The invention provides a drug-loaded sophorolipid microsphere, a preparation method thereof and application of the drug-loaded sophorolipid microsphere in preparation of a bionic cartilage scaffold. The preparation method of the drug-loaded sophorolipid microspheres comprises the following steps: (1) preparing a drug-containing sophorolipid solution; (2) preparing multiple emulsion; and (3) adding a horse radish peroxidase solution into the multiple emulsion for reaction to obtain the drug-loaded sophorolipid microspheres. The bionic cartilage scaffold sequentially comprises a bone layer, a transition layer and a cartilage layer from bottom to top, the bone layer is a cross-linked compound of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the transition layer is a physical mixture of nano-hydroxyapatite and drug-loaded sophorolipid microspheres; the cartilage layer is drug-loaded sophorolipid microspheres. The drug-loaded sophorolipid microspheres disclosed by the invention have good biocompatibility, excellent biocompatibility and good mechanical properties. The bionic cartilage scaffold utilizes the characteristics of the nHA and the drug-loaded sophorolipid microspheres to form a unique network structure, so that material failure caused by mechanical mismatching is avoided.

Nucleotide compositions, recombinant vectors, and recombinant viral genomes are disclosed that include a modified horseradish peroxidase (HRP) gene sequence, as well as recombinant viruses that have the modified HRP gene sequence incorporated into the viral genome thereof. The recombinant viruses are utilized in an HRP-based assay to analyze the neutralization potential of candidate antiviral agents, such as antiviral antibodies.

The invention relates to the technical field of biology, in particular to a polypeptide combination and application thereof. The invention provides a human giant cell IgM epitope polypeptide. The invention also provides a method for coupling the epitope polypeptide with horse radish peroxidase. The invention also provides a detection method of the epitope polypeptide coupled horseradish peroxidase applied to the human giant cell IgM detection kit. The method has the advantages that the two epitope polypeptides are identified for the first time to detect positive serum samples of human cytomegalovirus IgM, the human cytomegalovirus IgM detection kit adopting the polypeptides as specific antigens has excellent sensitivity and specificity, and the standard is easy to control.

The application provides a glucose detection test strip and a preparation method and application thereof. The preparation method of the glucose detection test strip comprises the following steps: taking copper sulfate pentahydrate, a phosphate buffer solution, glucose oxidase and horseradish peroxidase, mixing uniformly to obtain an enzyme-containing mixed solution; immersing a test strip in the enzyme-containing mixed solution, and obtaining an organic-inorganic hybrid nanoflower loaded test strip after reaction; immersing the organic-inorganic hybrid nanoflower loaded test strip in a mixed solution containing a chromogenic agent, a gel, bovine serum albumin and polyethylene glycol to obtain a glucose detection test strip. The preparation method of the glucose detection test strip has higher sensitivity and shorter response time.

The invention discloses an up-conversion nano-composite with a core-shell structure, a preparation method and application, and belongs to the technical field of biomedical bimodal nano-probes. According to the nanocomposite, up-conversion nanoparticles (UCNPs) serve as a core, metal-organic frameworks (MOFs) serve as a shell layer, horse radish peroxidase (HRP) is loaded on the shell layer, and UCNP (at) MOF / HRP is formed. Subsequent biological functional modification, such as coupling of an antibody, an antigen, nucleic acid and other substances, can be carried by an activating group or a metal coordination bond carried by a shell ligand of the upconversion nanocomposite MOF with the core-shell structure, so that the functions of specific recognition, targeted capture, specific loading and the like are realized.

The invention discloses a chemiluminescence immunoassay method and kit for detecting progestational hormone of pandas, and belongs to the technical field of biological detection. The specific process comprises the following steps: by taking goat anti-mouse IgG as a coating antibody, enabling a progestational hormone standard substance or a to-be-detected sample and horse radish peroxidase labeled progestational hormone to compete with a limited amount of progestational hormone antibodies together; after incubation and washing, a chemiluminescent substrate is added, and quantitative analysis of the progestational hormone in the panda urine or excrement is realized by detecting the intensity of a luminescent signal according to a standard curve. The invention provides a novel chemiluminescence immunoassay method, the sensitivity of the chemiluminescence immunoassay method is far higher than that of a traditional ELISA method (the lowest detection line can reach 31.25 pg / mL), the operation is simple and convenient, the detection is rapid, and the urgent demand on rapid and accurate determination of the hormone level in panda pregnancy monitoring and reproduction management can be met.

The invention provides a rapid detection kit and a rapid detection method for phytosterol, and belongs to the field of food detection.The rapid detection kit for phytosterol comprises a phytosterol standard substance, a standard substance diluent, a substrate solution, an enzyme mixed solution, a stop solution and an elisa plate; the standard substance diluent comprises isopropanol or dimethyl sulfoxide; the substrate solution is prepared from TMB (Tetramethylbenzidine) and a substrate buffer solution; the enzyme mixed solution is a mixed solution of a cholesterol oxidase solution and a horse radish peroxidase solution. By optimizing the composition of each working reagent in the kit, the sensitivity and accuracy of phytosterol detection are remarkably improved. Meanwhile, the rapid detection method provided by the invention is simple and convenient to operate, short in detection time and suitable for on-site rapid detection.

According to the hydrogel material disclosed by the invention, the gelatin / gallic acid conjugate with a specific concentration is taken as a base material, and the base material is cross-linked by utilizing a horse radish peroxidase / hydrogen peroxide combined enzyme catalysis system, so that an additional photo-thermal response material does not need to be added in the gelling process of the hydrogel; in the cross-linking process, the hydrogel serves as a cross-linked structure for forming the interior of the hydrogel and shows excellent photo-thermal responsiveness, and the hydrogel can respond to photo-thermal temperature rise to improve the self deformability, so that irregular wounds are better covered; therefore, the invention provides a hydrogel material which has good biological safety, oxidation resistance and bacteriostasis and can be used for wound injury repair.

The application discloses a high-stability horseradish peroxidase, a recombinant expression vector, a preparation method and application. The amino acid sequence of the horseradish peroxidase is shown as SEQ ID NO. 1. Compared with wild-type horseradish peroxidase, the enzyme activity of the horseradish peroxidase is increased by 3.2 times, and compared with commercially available horseradish peroxidase, the thermal stability is better. The method can produce the horseradish peroxidase by using a tobacco transient expression system. Compared with an Escherichia coli expression system and a yeast expression system, the tobacco transient expression system can correctly fold and glycosylate, and has higher enzyme activity and stability. The purity of the horseradish peroxidase obtained according to the method can reach RZ 3.2, the enzyme activity can reach 275 U / mg, and the horseradish peroxidase can be applied to immunological detection.

The present invention belongs to the field of biosensor instant detection technology, and relates to a DAAO / HRP@MAF-7 hydrogel and its preparation method and application. The hydrogel is formed by immobilizing horseradish peroxidase HRP and D-amino acid oxidase DAAO in a hydrophilic metal organic framework MAF-7, and then the prepared DAAO / HRP@MAF-7 biocomposite material and the color developer TMB are co-loaded in a PEG-DA hydrogel. The DAAO / HRP@MAF-7 hydrogel can be used as a biosensor to detect D-amino acids.

The present invention provides a high sensitivity immunoassay method for determining the concentration of an analyte in a sample based on interferometry. The present invention provides a high molecular weight conjugate comprising a plurality of streptavidin and horse radish peroxidase (HRP) and using the conjugate to increase the sensitivity of biochemical assays. The present invention relates to a method for preparing a high molecular weight conjugate comprising at least 2 HRP, preferably 4 HRP, and binding a precipitated HRP substrate to a solid phase to increase the signal of a biochemical assay.

The present invention provides a detection reagent for cardiac troponin I, comprising: a cTnI aptamer labeled with a first marker and a horseradish peroxidase covalent linker labeled with a second marker; the first marker and the second marker can be combined. The present invention also provides the application of the detection reagent, a detection kit and a detection method. The present invention uses a cTnI aptamer instead of a signal antibody to detect cTnI. As a molecular recognition element, nucleic acid aptamers have many excellent properties such as simple in vitro synthesis, good stability, and easy sequence design and modification, thereby reducing the difficulty of preparation while ensuring sensitivity. There is no need for complex and high-cost DNA amplification technology and nanomaterial synthesis and modification, and the method is simple and low-cost. Furthermore, the present application adds albumin as a supporting agent to the signal aptamer solution, which significantly improves the sensitivity, repeatability, applicability to complex samples and stability of the method.

The application relates to the field of biotechnology, and in particular to a wheat allergen specific IgE detection kit and a detection method thereof. The kit comprises magnetic particles, enzyme-labeled anti-human IgE antibodies and biotin-labeled wheat allergen. The wheat allergen is obtained by treating a wheat protein extract with heat treatment or a pretreatment agent. The pretreatment agent comprises one or more of sodium azide, L-cysteine and L-cysteine hydrochloride. The wheat allergen is biotin-labeled after specific pretreatment. The chemiluminescence immunoassay method uses streptavidin to prepare magnetic microspheres and horseradish peroxidase-labeled anti-human IgE antibodies. The method is fully automatic, ensures the accuracy of the detection result, and improves the test flux, the specificity and the sensitivity of the detection result.

The invention discloses a modified electrode based on a cyclic peptide-enzyme composite material and application of the modified electrode in hydrogen peroxide detection. The cyclic peptide-enzyme composite material modified electrode has good reproducibility and selectivity on hydrogen peroxide detection, cyclic tryptophan dipeptide (c-WW) is utilized for self-assembly to form spherical nano-particles, and the spherical nano-particles are combined with horse radish peroxidase (HRP) to form the cyclic peptide-enzyme nano composite material. The cyclopeptide-enzyme composite material is fixed on the surface of a glassy carbon electrode by using a Nafion solution, and the modified glassy carbon electrode Nafion / HRP-(c-WW NPs) / GCE is used as a working electrode, a platinum wire electrode is used as a counter electrode and a silver-silver chloride (Ag / AgCl) electrode is used as a reference electrode to form a three-electrode system, so that high-sensitivity detection of hydrogen peroxide is realized. The detection method provided by the invention has the characteristics of quick response, high sensitivity, high selectivity, low detection limit, good biocompatibility and the like on detection of hydrogen peroxide, and has a very good application prospect.

The invention provides photo-thermal response enhanced hydrogel loaded with black rice extract as well as a preparation method and application of the photo-thermal response enhanced hydrogel loaded with the black rice extract, and the photo-thermal response enhanced hydrogel is formed by taking a mixture of hyaluronic acid / tyrosine conjugate and the black rice extract as a base material under the cross-linking action of horse radish peroxidase / catalase, horseradish peroxidase acts on the black rice extract while serving as a cross-linking agent, the near-infrared photo-thermal responsiveness of the black rice extract after reaction of the horseradish peroxidase is greatly enhanced, and good oxidation resistance, inflammation resistance and photo-thermal responsiveness can be achieved only through a small amount of black rice extract in a hydrogel system.