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1628 results about "Standard product" patented technology

Definition of Standardized Product: A standardized product is a product that regardless of what company furnishes the good, consumers regard the product furnished by all the companies as identical. Examples of standardized products include agricultural products (such as grain and milk), most mined minerals, and fish.

Particle-enhanced turbidimetric immune assay kit for detecting adiponectin and preparation method thereof

The invention relates to a particle-enhanced turbidimetric immune assay kit for detecting adiponectin and a preparation method thereof, in particular to a detection method of a turbidimetric immune assay. The particle-enhanced turbidimetric immune assay kit for detecting the adiponectin comprises a kit body, an application liquid bottle and a latex suspension bottle coating an antiviral adiponectin monoclonal antibody, wherein the application liquid bottle is filled with application liquid; the application liquid comprises a surfactant, a preservative, a macromolecular accelerator, sodium chloride and a buffering agent; the latex suspension bottle coating the antiviral adiponectin monoclonal antibody is filled with a latex suspension coating the antiviral adiponectin monoclonal antibody; and the latex suspension coating the antiviral adiponectin monoclonal antibody comprises latex coating the antiviral adiponectin monoclonal antibody, the surfactant, a stabilizer and the buffering agent. The preparation method comprises the following steps of: preparing a latex antibody; preparing the latex suspension coating the antiviral adiponectin monoclonal antibody; preparing the applicationliquid; and finally preparing adiponectin series standard products.
Owner:ANHUI IPROCOM BIOTECH CO LTD

Method for simultaneously measuring multiple residues of organic chloride and pyrethroid pesticides

The invention discloses a method for simultaneously measuring multiple residues of organic chloride and pyrethroid pesticides. The method comprises the following working procedures: crushing a tobacco sample until grain diameters are less than 450 mu m; adding 10 to 20ml of extracting solution and internal standard solution into every program of tobacco sample; extracting the mixed solution by using ultrasonic wave; filtering the mixed solution by using a funnel filled with anhydrous sodium sulfate to obtain the extracting solution; adding the extracting solution into a Florisil solid extraction column for extracting and eluting; collecting an eluent; analyzing the eluent by using a gas chromatograph, wherein the analysis comprises the following conditions: adopting an Elite 5MS capillary column, high-purity N2 as a carrier gas, a constant flow mode in which the flow rate is 0.5 to 1.5 ml/min, a sample inlet temperature of between 280 and 320 DEG C, a detector temperature of between 330 and 370 DEG C, splitless sampling in which the sampling volume is between 1 and 2 mu L, and an initial temperature of 100 DEG C which rises from 100 to 180 DEG C at a speed of 8 DEG C/min, is kept for 4 minutes, rises from 180 to 270 DEG C at the speed of 5 DEG C/min and is kept for 15 minutes until all sample flows out completely; and determining the nature of the sample by using the retaining time of a standard sample and quantifying by using an internal standard method. In the method, ultrasonic wave is adopted for extracting and a solid extraction column is adopted for purifying, so that the method has the advantages of operating step simplification, high work efficiency, good purification effect, capability of simultaneously measuring multiple residues of 25 kinds of pesticides including 17 organic chloride pesticides of hexachlorocyclohexane, DDT and the like, seven pyrethroid pesticides such as fenpropathrin and the like, and one plant growth regulator.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI

System for electrochemical quantitative analysis of analytes within a solid phase and affinity chromatographic test strip

System, method, and test strip for solid phase, electrochemical, quantitative analysis of analytes contained in biological fluid samples. Preliminary to analysis, a test sample solution can be applied to a sample collection pad associated with the solid phase test environment of the test strip. The test sample solution and a test kit reagent are thereby initially contacted, under assay conditions, within this solid phase test environment, and caused to migrate along a fluid pathway therein. Irrespective of the assay format (competitive assay, sandwich assay, etc.), a test kit reagent (e.g. labeled substance) and the analyte of interest (e.g. proteins, hormones or enzymes, small molecules, polysaccharides, antibodies, nucleic acids, drugs, toxins, viruses or virus particles, portions of a cell wall and other compounds which have specific or characteristic markers that permit their identification), either interact with one another to form a complex, or, alternatively, compete with one another for interaction with another test kit reagent, resulting in the concentration of an indicator substance within a delimited area of the solid phase. Thereafter, the delimited area of the test strip is subjected to electrochemical analysis and the results determined by monitoring an electrochemical transition in the form of an indicator, or derivative of the indicator (e.g. indicator species), by potentiostatic or potentiometric quantitative analysis (e.g. anodic stripping voltammetry). This electrochemical transition of the indicator has a characteristic electrical fingerprint that can be measured and which, when compared to a standard, can be correlated with the concentration of the analyte in the sample. This method is suitable for the determination/monitoring of therapeutic range of drugs (anti-convulsants drugs), determination of critical and potentially dangerous levels of endogenous materials which are indicative of disease states (prostate cancer), and numerous other applications presently requiring elaborate and time-consuming clinical laboratory analysis.
Owner:INTEC SCI INC

Method for rapidly, accurately and repeatedly determining foot-and-mouth disease vaccine antigen 146S

The invention discloses a method for rapidly, accurately and repeatedly determining foot-and-mouth disease vaccine antigen 146S. A size exclusion high-efficiency liquid-phase chromatographic column in a molecular weight separation range of 2*10<4> to 1*10<7>Da is adopted to carry out the chromatographic separation on a detected sample on a high-efficiency liquid-phase chromatography. The operation pressure of the chromatography is 1.0MPa to 2.5MPa, the flow rate in the chromatographic column is 0.5 to 1.0 ml/min, a flow phase is phosphate buffer (pH 7.0 to 7.5) containing 0.1M sodium sulfate, and the column temperature is 15 to 25 DEG C. An ultraviolet and laser detector is used for detecting an optical signal of effluent at an outlet of the size exclusion high-efficiency liquid-phase chromatographic column, and a peak area of a sample can be analyzed by virtue of a computer software system of the high-efficiency liquid-phase chromatography. A standard curve of the absorption peak area and 146S concentration is established by virtue of a relation between the ultraviolet absorption peak and the concentration of different 146S standard products of different concentrations. Chromatograph is carried out on the detected sample through the size exclusion high-efficiency liquid-phase chromatographic column. The ultraviolet absorption peak area is measured, and the concentration of 146S in the detected sample can be acquired according to the standard curve.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI

Quick qualitative and quantitative method for oligosaccharide in breast milk

The invention discloses a quick qualitative and quantitative method for oligosaccharide in breast milk. The quick qualitative and quantitative method mainly includes steps of 1, pretreating samples, to be more specific, removing fat and proteins from 150-250 micro-l of breast milk to obtain ultimate supernatant, adding ultra-pure water into the supernatant and diluting the supernatant to obtain the loaded samples; 2, establishing standard curves for standard substances by the aid of ultrahigh-performance liquid chromatography and mass spectrometry; 3, separating different components of the oligosaccharide in the breast milk in the loaded samples by the aid of ultrahigh-performance liquid mass spectrometry and carrying out quantitative analysis by the aid of mass spectrometry combined with the standard curves so as to obtain the content of the oligosaccharide in the breast milk. The ultrahigh-performance liquid chromatography is implemented by the aid of amino chromatographic columns with the sizes of 2.1*100 mm and 1.7 micrometers, 8-10 mmol/L of ammonium formate solution (A) and acetonitrile (B), and the ammonium formate solution (A) and the acetonitrile (B) are used as mobile phases; gradient elution programs are carried out by the aid of 95%-75% of B for 0-10 min or are carried out by the aid of 75% of B for 10-15 min or are carried out by the aid of 75%-65% of B for 15-20 min or are carried out by the aid of 65%-10% of B for 20-21 min or are carried out by the aid of 10% of B for 21-24 min or are carried out by the aid of 10%-95% of B for 24-25 min or are carried out by the aid of 95% of B for 25-35 min; the flow rates are 0.3 mL/min, and the column temperatures are 40-60 DEG C. The quick qualitative and quantitative method has the advantage that 12 types of oligosaccharide in the breast milk can be quickly detected by the aid of the quick qualitative and quantitative method and can be quantified by the aid of the quick qualitative and quantitative method.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI

Method for identifying tea-leaf origin by infrared spectrum

The invention provides a method for identifying production place of tea by using infrared spectrum technology, which comprises the following steps: production place typical lines of tea in place of origin are constructed; B. detecting sample: tea samples to be detected are taken and detected under the same condition with the standard sample respectively to obtain standard mid-infrared spectrums, average deviations between the tea samples and reference tea in production place are calculated according to the spectrums, and difference values between the average deviations and the production place typical lines are calculated, if the difference value is within standard deviation range, the tea sample belongs to the production place, otherwise, the tea sample does not belong to the production place. The method has the following advantages: 1. the analyzing result is not affected by subjective consciousness, thereby providing an impersonal evaluation criterion; 2. according to similarity theory, the average deviation can represent the difference between infrared spectrums of tea more accurately; 3. component separation, extraction and the like on tea are not needed before detecting infrared spectrum of the tea, which simplifies the operation, greatly improves the analyzing speed and reduces the detecting cost without environment pollution.
Owner:HEBEI UNIVERSITY

Method for measuring vitamins A, D and E in compound vitamin

The invention discloses a method for measuring vitamins A, D and E in a compound vitamin. The method comprises the following steps: (1) preparing solution of a sample to be measured; (2) preparing standard substance solution; (3) taking the solution of the sample to be measured, separating the solution prepared in the step (2) with a Waters reversed phase C18 chromatographic column and detecting the solution with a diode array detector to obtain a corresponding chromatogram and qualitatively deciding various vitamins in the solution of the sample to be measured according to the retention time of various vitamins in the standard substance solution; (4) respectively obtaining the mass concentrations of various vitamins in the solution of the sample to be measured via the proportional relationships of the peak areas of various vitamins in the chromatogram according to the concentrations of various vitamins in the standard substance solution; and (5) computing the contents of the vitamins in the solution of the sample to be measured through a formula according to the concentrations. The method has the following advantages: the method has the characteristic of simple, convenient, rapid and accurate measurement; and the analysis time is shortened and the organic reagent consumption is reduced while control of the raw materials of the health food is implemented.
Owner:TIANJIN TIANSHI BIOLOGICAL DEV +2

Series quadrupole-rod gas-chromatographic mass spectrometry detection method for 35 toxic medicaments in urine

The invention discloses a series quadrupole-rod gas-chromatographic mass spectrometry detection method for 35 toxic medicaments in urine. The series quadrupole-rod gas-chromatographic mass spectrometry detection method comprises the steps of constructing a multiple-reaction detection method on a series quadrupole-rod gas chromatograph-mass spectrometer through standard substances of 35 toxicants (medicines), and determining 2-3 pairs of feature parent ions and daughter ions and the retention time of each toxicants (medicines); extracting a urine sample to be detected through diethyl ether, performing ultrasonic and centrifugal treatment, blow-drying and dissolving, and then qualitatively and quantitatively analyzing the toxicants (medicines) through a tandem mass spectrum MRM. By the adoption of a series quadrupole-rod gas-chromatographic-mass spectrometry/mass spectrometry method (GC-MS/MS), the method disclosed by the invention can quickly screen 35 conventional toxicants (medicines) in the urine of a human body; a detection result is accurate, sensitive and quick; the recycling rate of toxicants (medicines) is 80.82-118.56 percent; the detection limit is up to 0.0014-1.8 micrograms per microliter.
Owner:FUJIAN INT TRAVEL HEALTH CARE CENT

Targeted metabo lomics analysis method for determining metabolites of living body

The invention provides a targeted metabo lomics analysis method for determining metabolites of a living body. The targeted metabo lomics analysis method can simultaneously determine 700 main metabolites of a living body based on series connection of ultra-high performance liquid chromatography and triple quadrupole and comprises the following steps: firstly, 700 main metabolites in a living body are screened; standard products of compounds are optimized to obtain optimal parent ions, daughter ions and mass spectrography conditions of the metabolites; the metabolites in samples are extracted by methanol, acetonitrile and water; liquid chromatograph conditions are optimized; a hydrophilic interaction is adopted to separate the metabolites; the separated metabolites enters a mass spectrum; the 700 main metabolites are determined at the same time by use of a real-time scheduled multi-reaction monitoring mode with quick positive and negative polarity switching function; the metabolite differences between all the samples are analyzed via partial least square analysis; changes of the metabolic pathway are found by pathway enrichment analysis. The targeted metabo lomics analysis method remarkably increases the number of detected compounds, and greatly shortens the analysis time.
Owner:TIANJIN SUNNYPEAK BIOTECH CO LTD
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