Method for measuring vitamins A, D and E in compound vitamin

Abstract

The invention discloses a method for measuring vitamins A, D and E in a compound vitamin. The method comprises the following steps: (1) preparing solution of a sample to be measured; (2) preparing standard substance solution; (3) taking the solution of the sample to be measured, separating the solution prepared in the step (2) with a Waters reversed phase C18 chromatographic column and detecting the solution with a diode array detector to obtain a corresponding chromatogram and qualitatively deciding various vitamins in the solution of the sample to be measured according to the retention time of various vitamins in the standard substance solution; (4) respectively obtaining the mass concentrations of various vitamins in the solution of the sample to be measured via the proportional relationships of the peak areas of various vitamins in the chromatogram according to the concentrations of various vitamins in the standard substance solution; and (5) computing the contents of the vitamins in the solution of the sample to be measured through a formula according to the concentrations. The method has the following advantages: the method has the characteristic of simple, convenient, rapid and accurate measurement; and the analysis time is shortened and the organic reagent consumption is reduced while control of the raw materials of the health food is implemented.

Description

technical field [0001] The invention relates to a method for determining vitamins A, D and E in multivitamins of health food. Background technique [0002] Vitamins are essential nutrients to maintain the normal physiology of humans and animals. Although humans and animals have little demand for vitamins, their functions are very important. Because people cannot synthesize vitamins, they must rely on food to provide enough vitamins. With the development of society, more and more vitamin-containing products appear on the market, and more and more health products are labeled as rich in various vitamins. However, how many vitamins are contained in a certain vitamin-containing product, the method of determining vitamins in food is very important. [0003] In recent years, with the development of society, there are more and more products containing multivitamins, and the dosage forms include tablets or granules. Most of the vitamins in food are added in the form of multivitamins...

AI Technical Summary

Problems solved by technology

Using the saponification method to process the multivitamin samples will lead to serious loss of vitamins A, D, and E in the multivitamins, which will cause the test results to not truly reflect the vitamin content in the sample and affect the accuracy of the test data.

[0006] 3. Poor reproducibility and low accuracy;

[0007] 4. Reagent consumption is large

[0008] Due to the different polarities of vitamins A, D, and E, vitamins A, D, and E cannot be detected simultaneously by existing technologies. Usually, vitamins A and E are separated by reversed-phase chromatography, detected by an ultraviolet detector, and internal standard Quantitative determination of vitamin A and vitamin E by method, vitamin D is separated by normal phase chromatographic system and then detected by reversed phase chromatographic system

The disadvantages of this detection method are: the life of the normal phase chromatographic column is short, the price is expensive, and it is cumbersome to use the two chromatographic systems of normal phase and reverse phase alternately, which increases the loss of samples and reduces the detection accuracy.

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