286results about How to "Efficient enrichment" patented technology

The invention discloses a composition for preventing hair loss, controlling oil and removing dandruff and a preparation method thereof, and particularly belongs to the field of preparations for dressing and makeup. The composition for preventing hair loss, controlling oil and removing dandruff comprises the following components: 2-5 parts of a folium artemisiae argyi extract, 1.1-1.6 parts of a ginger root extract, 5-10 parts of a polygonum multiflorum extract and 4-6 parts of a cacumen biotae extract. The polygonum multiflorum extract prepared by the method disclosed by the invention has better bacteriostatic activity, and compared with single strain fermentation, the mixed strain fermentation can more effectively enrich active ingredients in polygonum multiflorum; the anti-hair-loss, oil-controlling and anti-dandruff composition provided by the invention has excellent oil-controlling and oil-inhibiting effects; the shampoo provided by the invention has remarkable effects of preventing hair loss and strengthening hair.

The preparation method comprises the following steps: mixing a tannic acid solution and a tetravalent cerium salt solution, adjusting the pH value to 9-11, and reacting to obtain the cerium tannate nano-enzyme, the molar ratio of the tannic acid to the tetravalent cerium salt is (1-4): 1; the hydration dynamic diameter of the cerium tannate nano-enzyme is 20 to 80 nm. The TA-Ce nano-enzyme prepared by the preparation method disclosed by the invention is smaller in particle size, can keep stable and good dispersibility in a physiological environment, and has good biocompatibility, low hemolysis rate and small systemic toxicity; meanwhile, the compound has good kidney targeting property, can be effectively enriched into the injured kidney, eliminates various ROS (reactive oxygen species) and relieves oxidative stress by utilizing unique redox cycle between Ce < 3 + > and Ce < 4 + > states, and has a good effect in treating acute kidney injury induced by rhabdomyolysis and / or cis-platinum. The research lays a foundation for the metal polyphenol nano material in the treatment of acute kidney injury.

The invention discloses a CTC cell detection method based on a micro-fluidic chip, and belongs to the technical field of cell treatment. In order to solve the problems of high false positive and false negative rates, time consumption and low efficiency caused by difficulty in accurately distinguishing CTC from other cell types, the invention can efficiently and specifically capture CTC cells, reduce the possibility of false positive and false negative and improve the detection accuracy by constructing a specific capture chamber and filling specific antibodies and immunomagnetic beads. The multi-channel design allows a plurality of samples to be processed at the same time, the detection efficiency is improved, the micro-fluidic chip technology integrates a plurality of steps on one platform, automation and standardization of operation are achieved, personal errors are reduced, the stability and repeatability of detection are improved, the uniformity and stability of the detection process are ensured, and the detection efficiency is improved. The fluorescence imaging technology clearly displays CTC and obtains key parameters such as quantity, form and fluorescence intensity of CTC, thereby providing powerful support for cancer diagnosis and research.

The invention provides a gradient recycling method for germanium, lead, zinc and iron in hard zinc slag, and belongs to the technical field of extraction of valuable elements in lead-zinc smelting waste. The method comprises the eight working procedures of reduction smelting, fuming volatilization, acid making, leaching, reduction germanium precipitation, two-stage pyrolysis, chlorination distillation and rectification, lead in the hard zinc slag can be recycled in the form of metal lead through reduction smelting, zinc and germanium are enriched in the form of smoke through fuming volatilization, iron is enriched in water-quenched slag, zinc and germanium are leached in a solution, then tannin is used for germanium precipitation, and after the tannin is used for germanium precipitation, the two-stage pyrolysis is carried out. According to the method, germanium can be completely enriched in tannin germanium residues, tannin germanium is pyrolyzed to obtain different products, a pyrolysis product washing is subjected to chlorination distillation to obtain crude germanium tetrachloride, and the crude germanium tetrachloride is rectified to obtain refined GeCl4. According to the method, the direct recovery rate of lead can reach 98%, the direct recovery rate of zinc can reach 96.92%, the recycling rate of iron can reach 99%, and the direct recovery rate of germanium can reach 95.04%.

The invention discloses a multi-metal borate nano-particle as well as a preparation method and application thereof. The preparation method comprises the following steps: in the presence of small molecules with alkyl chains, performing high-temperature thermal decomposition on an organic metal compound or a metal salt compound precursor and organic borate in a solvent, and then centrifuging, modifying, performing ultrafiltration, dialyzing and purifying to obtain the multi-metal borate nanoparticles. The preparation method disclosed by the invention has the characteristics of mildness, high efficiency and strong controllability. The multi-metal borate nanoparticles prepared by the preparation method disclosed by the invention have the advantages of monodisperse size, relatively high magnetic property and good biocompatibility. Therefore, the prepared multi-metal borate nanoparticles not only have multi-modal imaging functions such as magnetic resonance imaging, near-infrared two-region imaging, nuclear medicine imaging and magnetic particle imaging, but also can be used for various treatment means such as magnetocaloric therapy and boron neutron capture therapy; potential advantages are realized in biomedical applications such as magnetic targeting tumor treatment and imaging contrast agents, and high practical values are realized.

The invention belongs to the technical field of medicine detection, and particularly relates to a high-sensitivity liquid chromatography method for detecting mutagenic impurities in atomoxetine hydrochloride key starting material 3-chlorobenzene propanol, the method adopts an HPLC (High Performance Liquid Chromatography) analysis method, a chromatographic column adopts octadecylsilane chemically bonded silica as a filler, and the composition of a mobile phase A is a buffer solution; the mobile phase B is methanol or acetonitrile; carrying out gradient elution on the mobile phase; the flow velocity is 0.4 to 0.8 ml / min; the detection wavelength is 230 to 250 nm; by adopting an HPLC analysis method, the content of mutagenic impurities in the key starting material 3-chlorobenzene propanol of atomoxetine hydrochloride can be effectively separated and measured, and the method is high in sensitivity, strong in specificity and good in accuracy and repeatability, and has extremely important significance on quality control of the key starting material 3-chlorobenzene propanol of atomoxetine hydrochloride.

The invention discloses a method for preparing a battery-grade vanadyl sulfate electrolyte by using a vanadium leachate, which comprises the following steps: S1, carrying out anion resin adsorption-desorption treatment and reduction treatment by using the vanadium leachate with the vanadium ion concentration of 1.0-20g / L as a stock solution and using a sulfurous acid solution or a saturated SO2 solution as a desorption solution to obtain a vanadyl sulfate solution with the vanadium ion concentration of 20-40g / L; the method comprises the following steps: sequentially performing extraction and back extraction on a vanadyl sulfate solution with the vanadium ion concentration of 20-40g / L to obtain a vanadyl sulfate electrolyte with the vanadium ion concentration of 80-160g / L; and S3, adsorbing the vanadyl sulfate electrolyte with the vanadium ion concentration of 80-160g / L by using an adsorbent, and filtering to obtain the battery-grade vanadyl sulfate electrolyte. According to the method, the removal of impurities in the vanadium leachate and the efficient reduction conversion and enrichment purification of vanadium ions can be simultaneously / synchronously realized, and the technical effects of improving the product quality, shortening the process flow, improving the production efficiency and reducing the production cost are achieved.

The invention provides a perovskite quantum dot with a fluorine-containing passivation layer, the perovskite quantum dot comprises a micro / mesoporous template with incompletely closed pores, perovskite nanocrystals and the fluorine-containing passivation layer grow in pores of the micro / mesoporous template, and the fluorine-containing passivation layer is used for passivating surface defects of the perovskite nanocrystals; and the fluorine-containing passivation layer comprises BaF2 (BaF2). According to the method, the surface of the perovskite nanocrystal can be passivated without collapsing the micro / mesoporous template, so that the stability is improved.

The utility model discloses an enrichment device for anaerobic ammonium oxidation bacteria. The device comprises an enrichment unit and a control unit, the enrichment unit comprises one or more culture tanks and carriers fixed in the culture tanks; a liquid inlet and a liquid outlet are respectively formed in a tank body of the culture tank; the one or more culture tanks are connected in sequence; the culture tank is filled with a nutrient medium; the nutrient medium circularly flows in one or more culture tanks to form an aerobic and anaerobic environment. The enrichment device for the anaerobic ammonium oxidation bacteria is independently arranged outside a sewage treatment system, efficient enrichment is achieved, and the problem that the growth and reproduction of the anaerobic ammonium oxidation bacteria are slow due to the fact that the anaerobic ammonium oxidation bacteria are affected by pollutants in the sewage treatment system in the redebugging period is solved.

The invention relates to the field of extraction and purification of active substances of natural products, and discloses plant active ingredients in camphor tree seed kernels and a separation and purification method thereof.The method comprises the steps that fresh camphor tree seed kernels and water are mixed, wet-process superfine grinding is conducted through a colloid mill at the room temperature, centrifugal separation is conducted to obtain a middle-layer water phase, and the middle-layer water phase is statically or dynamically adsorbed through HJ-18 macroporous resin; and desorbing with 80% ethanol, collecting a desorption solution, carrying out rotary evaporation, and freeze-drying to obtain the water-soluble active component of the camphor tree seed kernel. The invention provides a new process for enriching and purifying the active components of the camphor tree seed kernels, the active components of the camphor tree seed kernels are enriched and purified by taking water as an extracting agent and combining with macroporous resin, the operation is simple and convenient, the enrichment efficiency is high, the use amount of organic solvents such as methanol and ethanol is reduced, and the process is safe and environment-friendly. In addition, compared with an ethanol extraction method, the method has the advantages that the camphor tree seed kernel protein is not denatured, and the quality of the camphor tree seed kernel protein is improved; according to the technical scheme, a new solution is provided for comprehensive utilization of camphor tree seed resources.

The invention relates to the field of biological detection, in particular to a pretreatment kit for a positive sample containing chemical magnetic beads and application of the pretreatment kit. According to the method, the chemical magnetic beads are creatively applied to mass spectrum identification, and the strong adsorption capacity of the chemical magnetic beads to bacteria is utilized, so that the cost can be saved, and the pretreatment time of mass spectrum detection is greatly shortened. Meanwhile, an incubation solution is used for replacing a sample, so that the environment of bacteria is simpler, efficient adsorption of the magnetic beads to the bacteria is more facilitated, a bacteria-chemical magnetic bead compound can be extracted through simple magnetic adsorption, then the bacteria on the bacteria-chemical magnetic bead compound are eluted and detected, the purity of detected mycoprotein is very high, species identification can be achieved through mass spectrum, and the method is suitable for large-scale popularization and application. A new thought is provided for mass spectrum microorganism detection.

The invention discloses a method for recovering valuable metals in a retired ternary lithium battery based on cascade separation of a deep eutectic solvent and oxalic acid. The method comprises the following steps: 1) stirring and leaching the deep eutectic solvent and black powder at 110-130 DEG C for 3-4 hours according to a liquid-solid ratio of (5-50): 1 (ml: g) to obtain a leaching solution; (2) measuring the contents of lithium, cobalt, nickel and manganese in the leachate according to ICP (Inductively Coupled Plasma), adding oxalic acid exceeding the required amount according to the contents of lithium, cobalt, nickel and manganese, stirring, standing, and centrifuging to obtain a precipitate mixture of nickel oxalate precipitate, cobalt oxalate precipitate and manganese oxalate precipitate and lithium filtrate; and (3) repeating the steps (1)-(2) for at least three times by taking the lithium filtrate obtained at each time as the eutectic solvent, performing rotary evaporation on the obtained final lithium filtrate, adding sodium carbonate, and centrifuging to obtain lithium carbonate precipitate. The purity of the ternary lithium precursor prepared from cobalt, nickel and manganese obtained through the method can reach 99% or above, the purity of obtained lithium carbonate reaches 95% or above, and closed-loop recycling is achieved.

The invention belongs to the technical field of environmental protection, and discloses a method for rapidly and efficiently removing and recycling polycyclic aromatic hydrocarbon in water and a carbon-based adsorbent, the method comprises the following steps: S1, preparing a modified carbon-based adsorbent; s2, treating the polycyclic aromatic hydrocarbon pollutant wastewater; and S3, recycling the polycyclic aromatic hydrocarbon. According to the preparation method, H3PO4 or ZnCl2 is taken as an activating agent, and an activated carbon base material and an activating process are controlled, so that nano-micron multi-scale modified carbon-based adsorbent particles which are mainly prepared from a large number of long-strip-shaped gap mesopores which are communicated with one another and are approximately distributed in parallel and are auxiliary from a small number of discrete irregular groove-shaped mesopores are obtained; the long and narrow long-strip-shaped gap mesopores of the particle are matched with the irregular groove-shaped mesoporous structure, so that large-size polycyclic aromatic hydrocarbon can be quickly adsorbed and desorbed, and the balance of adsorption and desorption efficiency can be realized; on one hand, the adsorption and desorption efficiency of pollutants is improved, and on the other hand, the dosage of the adsorbent is greatly reduced.

The invention relates to the field of biological detection, in particular to a positive sample pretreatment kit and application thereof. The method comprises the following steps: providing a simple survival environment for bacteria by utilizing incubation liquid, and then utilizing the interaction of opposite electric properties between chemical magnetic beads and the bacteria, that is, when the bacteria in a positive sample are negatively charged, the chemical magnetic beads are positive magnetic beads; or when the bacteria in the positive sample are positively charged and the chemical magnetic beads are negative electromagnetic beads, the bacteria-magnetic bead compound is obtained, the operation is simple, the cost is low, the enrichment efficiency is high, the impurity interference resistance is high, the activity state of the enriched bacteria is not changed, the method can be used for subsequent mass spectrum identification and metabolic activity analysis, the application range is wide, and the method is suitable for popularization and application. Various in-vitro samples can be universally enriched, and the method has great significance on the development of the IVD field.

The invention relates to a primer composition and a method for whole genome sequencing of American type porcine reproductive and respiratory syndrome virus and application of the primer composition and the method. The sequences of the primer composition are as shown in SEQ ID No. 1 to SEQ ID No. 35. According to the sequencing method, RNA (Ribonucleic Acid) of a sample to be detected is taken as a template to perform reverse transcription reaction, the primer combination is adopted to perform PCR (Polymerase Chain Reaction) amplification on the whole genome of the American type porcine reproductive and respiratory syndrome virus, and an amplification product is subjected to three-generation sequencing library building and sequencing to perform species identification and genetic typing. The full-length PCR detection method is high in specificity and sensitivity, the efficiency of detecting the whole genome of the virus is greatly improved, rapid and accurate detection of the American type porcine reproductive and respiratory syndrome virus is achieved, accurate typing and evolutionary analysis can be achieved by utilizing the assembled whole genome, and the method is suitable for large-scale popularization and application. Traceability of the reproductive and respiratory syndrome virus is realized, and the method is of great significance to early screening, prevention and control of the American porcine reproductive and respiratory syndrome virus.

The invention relates to the technical field of nucleic acid extraction and purification, in particular to a DNA and RNA co-extraction reaction reagent and an extraction method, the reaction reagent comprises a nucleic acid protective agent RP, a sample lysis binding solution LB, a protein washing solution WB1, a nucleic acid rinsing solution WB2 and a nucleic acid eluent EB; the nucleic acid protective agent RP comprises DTT, mercaptoethanol, TCEP and sodium hydroxide; the sample cracking binding solution LB comprises Tris-HCl, benzenesulfonic acid, sodium citrate, guanidine hydrochloride, guanidine isothiocyanate, guanidine thiocyanate, urea, triton 100, SDS, potassium chloride, sodium chloride, bromhexine, N-acetylcysteine, ambroxol hydrochloride, isopropanol and absolute ethyl alcohol; the protein washing liquid WB1 comprises Tris-HCl, benzenesulfonic acid, sodium citrate, guanidine hydrochloride, guanidine isothiocyanate, guanidine thiocyanate, urea, triton 100, sodium chloride, isopropanol and absolute ethyl alcohol; the nucleic acid rinsing liquid WB2 is prepared from Tris-HCl, sodium chloride, isopropanol and absolute ethyl alcohol; and the nucleic acid eluent EB is nuclease-free water. The reaction reagent can be used for rapidly and sufficiently enriching and purifying DNA and RNA of a sample at the same time.

The invention relates to an anaerobic ammonia oxidizing bacteria enrichment optimization control method based on ecological niche regulation and control, which comprises the following steps: S1, selecting key ecological niche indexes of a constructed wetland as investigation factors, performing difference setting on the investigation factors, forming experimental systems of different ecological niche, controlling and monitoring the constructed wetland, and collecting data; s2, taking the relative abundance and / or absolute abundance of the anaerobic ammonia oxidizing bacteria collected in the step S1 as dependent variables, taking key ecological niche indexes as independent variables, and performing fitting so as to predict a model; and S3, performing prediction through the prediction model, comparing a prediction result with a control target, and performing optimization control on the anaerobic ammonia oxidizing bacteria enrichment of the constructed wetland according to a comparison result. According to the method, the prediction model with relatively high prediction accuracy can be obtained, so that the enrichment of anaerobic ammonia oxidizing bacteria can be ensured, the sewage treatment efficiency can be improved and a relatively good total nitrogen removal effect can be obtained by adjusting ecological niche indexes in the constructed wetland by virtue of the prediction model.

The invention relates to the technical field of normal-temperature yoghurt, in particular to normal-temperature yoghurt and a preparation method thereof. By combining double-frequency ultrasonic treatment and ultrahigh pressure treatment and applying ultrasonic treatment in the second-stage fermentation process between the first-stage fermentation and the third-stage fermentation, the drawing characteristic of the yoghourt can be greatly improved on the basis of maintaining relatively low bacterial count and even sterility of the yoghourt; the yogurt can be stored at normal temperature and has a good wire drawing effect. Excessive high-pressure treatment pressure or too long treatment time can cause damage to the structure of the yogurt and incapability of drawing the yogurt, and too low high-pressure treatment pressure and too short treatment time can cause excessive lactic acid content of the yogurt, so that the yogurt cannot be stored at normal temperature and only can be stored at low temperature. According to the invention, the pressure and time of the ultrahigh pressure treatment are controlled within the range, so that a good wire drawing effect is obtained, and the low bacterial count and even sterility of the yoghourt are maintained.

The invention discloses a near-infrared light nano probe as well as a preparation method and application thereof. The preparation method of the near-infrared nano probe comprises the following steps: dissolving polylactic acid-co-acetic acid in an acetonitrile solution, and adding lecithin and 1, 2-dipalmitoyl-sn-glycerol-3-phosphatidyl glycol to obtain a solution A; dissolving indocyanine green in an ethanol solution to obtain a solution B; and mixing the solution A and the solution B, and carrying out ultrasonic treatment to obtain the near-infrared nano probe. The near-infrared light nanoprobe can be used for imaging diagnosis of acute kidney injury, and effectively solves the problems of long diagnosis time, poor stability, high in-vivo clearance rate, low cell absorption rate and the like. Compared with existing ICG nano-particles, the ICG nano-particles are high in biological tissue penetrability, high in resolution ratio, low in phototoxicity and good in fluorescence imaging effect in an acute kidney injury model and have wide application prospects in AKI clinical diagnosis.

The invention discloses a method for recycling target metal in a lithium battery positive electrode material. The method comprises the following steps: pretreating a lithium battery to obtain positive electrode material particles; the particles are added into a composite solvent composed of citric acid and a dinitrogen heterocyclic ligand, target metal ions are leached out, and a leaching solution is obtained; adding a magnetic adsorbent of which the surface is modified with a phosphate group and / or a sulfonic acid group to enrich the target metal ions on the adsorbent; and applying a magnetic field to separate and enrich the magnetic adsorbent of the target metal ions. According to the method for recycling the target metal in the positive electrode material of the lithium battery, provided by the invention, the target metal ions can be efficiently leached by using a composite solvent system consisting of the citric acid and the organic ligand containing the two nitrogen heterocycles; meanwhile, a magnetic adsorbent with the surface modified with a phosphate group or a sulfonic group is adopted, efficient enrichment of target metal ions is achieved, and rapid separation can be achieved under an external magnetic field; and the recovery rate and the recovery purity of the method are relatively high.

The invention discloses a flexible three-dimensional MOF (Metal Organic Framework) array SERS (Surface Enhanced Raman Scattering) substrate and a preparation method thereof. The flexible three-dimensional MOF array SERS substrate comprises a flexible substrate, a three-dimensional MOF periodic array layer and a gold nanoparticle film which are sequentially arranged from bottom to top. The three-dimensional ordered MOF periodic array structure on the substrate can provide more attachment points for the gold nanoparticles, and high-density hot spots are formed; meanwhile, the MOF material has the advantages of high porosity, large specific surface area, adjustable aperture and the like, and can adsorb and capture a target analyte, so that to-be-detected molecules are concentrated and enriched in a hot spot region, and finally, the SERS activity is improved. The SERS substrate is a flexible substrate and can be directly attached to the surface of a complex to-be-detected object, and in-situ detection is achieved. The method has the characteristics of high sensitivity, good repeatability and wide applicability, and provides important support for rapid detection in food safety and environment monitoring.

The invention belongs to the technical field of immune cell culture, and particularly relates to an in-vitro amplification culture method of NK cells. The amplification method comprises the steps of CD16 pretreatment, plasma inactivation, lymphocyte separation and multi-stage fluid infusion. The preparation method comprises the following steps: pretreating CD16 in a T75 culture flask, promoting attachment and activation of NK cells, simulating a natural activation state, improving proliferation efficiency, treating whole blood and inactivating plasma to eliminate immunosuppressive factors, reserving nutritional ingredients, supporting amplification of the NK cells, enriching the NK cells through a lymphocyte separating medium, removing impurities and improving purity, mixing an RPMI-1640 culture medium with a solution A, and culturing for 12-24 hours at a high temperature; oK432 and IL-15 are added, the microenvironment is optimized, growth of the NK cells is remarkably promoted, liquid supplementing operation and bottling and bagging are carried out for multiple times, sufficient space and nutrition are provided, the cells are gradually transferred into a serum-free culture medium, and the cell safety and clinical application potential are improved.

The invention discloses a Fe3O4-COOH (at) ZIF-8-pDA (at) MIPs composite material as well as a preparation method and application thereof. The material comprises carboxyl functionalized magnetic MOFs (Fe3O4-COOH (at) ZIF-8) and a synthesized molecularly imprinted composite material (Fe3O4-COOH (at) ZIF-8-pDA (at) MIPs) loaded on the carboxyl functionalized magnetic MOFs (Fe3O4-COOH (at) ZIF-8). The application of the composite material is that the composite material is applied to pretreatment and enrichment of macrolide antibiotics in an environmental water sample by a magnetic solid phase extraction (MSPE) technology. According to the molecularly imprinted composite material disclosed by the invention, rapid, sensitive and strong-specificity extraction can be realized by using a small amount of adsorbent and sample in a short time, and the molecularly imprinted composite material is economical and applicable. According to the method, the adsorption extraction agent can be screened, designed, regulated and controlled according to the structure of the target analyte, a new thought is provided for analysis of other environmental pollutants, and the method is of great significance to environmental risk assessment, pollutant control and public health.

The invention belongs to the technical field of biology, and discloses a construct for preparing circular RNA, a kit, a preparation method, circular RNA and application. The construction body provided by the invention comprises an adenine-rich marker sequence, the length of the marker sequence is 20-120nt, and the content of adenine or complementary basic groups of the adenine is 75-100%. Due to the introduction of the marker sequence, the space structure of the construct can be optimized, the self-shearing catalytic activity of the construct is improved, and the cyclization efficiency is effectively improved; meanwhile, due to the arrangement of the marker sequence, the circular RNA and other non-cyclized RNA can be distinguished through ligo (dT) or ligo (dA), so that positive screening or negative screening of the circular RNA can be carried out by only utilizing a fixing agent comprising ligo (dT) or ligo (dA) in the preparation of the circular RNA, rapid and efficient enrichment of the circular RNA is realized, and the marker sequence has the potential of being applied to large-scale industrial production of the circular RNA and has a good application prospect.

The invention discloses a method and system for recycling CL-20 in strong acid waste liquid through macroporous adsorption resin, and belongs to the technical field of environmental remediation. The method comprises the steps that the macroporous adsorption resin is added into the strong acid waste liquid containing CL-20, and an adsorption reaction is conducted under set conditions; after the adsorption is completed, separating the resin saturated by adsorption from the waste liquid, and treating the resin saturated by adsorption by utilizing a desorption agent; putting the desorbed resin into the waste liquid containing CL-20 again for adsorption, and repeating adsorption-desorption; and treating the solution containing CL-20 obtained by adsorption-desorption to obtain a CL-20 solid. According to the method disclosed by the invention, the CL-20 adsorption rate can reach 97% or above under the condition that the adding amount of the resin is 10g / L, the concentration of the CL-20 in ethyl acetate after three times of circulation can reach 2.60 g / L, the content of the CL-20 in the finally recovered solid is 37.87%, and the total recovery rate reaches 58.25%. The invention provides an efficient, economic and environment-friendly CL-20 recovery method, the treatment problem of the CL-20-containing strong acid waste liquid can be effectively solved, and meanwhile, the recycling of valuable chemicals is realized.

The invention provides a method for detecting environmental pollutants by using a dispersive liquid-liquid microextraction technology. The method is particularly suitable for detecting endocrine disrupters in a urine sample. According to the method, a proper amount of a NaCl solution, a dispersing agent acetonitrile and an extracting agent trichloromethane are added into a urine sample, and target pollutants are rapidly extracted; and after centrifugation and nitrogen blowing drying, redissolving by using a methanol-water mixed solution in a ratio of 1: 1, and finally detecting and quantitatively analyzing through UHPLC (Ultra High Performance Liquid Chromatography). Compared with liquid-liquid extraction and solid-phase extraction, the method has the characteristics of simplicity and convenience in operation, high extraction efficiency and high detection sensitivity, is suitable for detecting bisphenol compounds in various matrixes, conforms to the green chemistry principle, and reduces the use amount of organic solvents. The method can be widely applied to detection and analysis of trace pollutants in environmental samples, and provides reliable technical support for environmental monitoring and pollutant risk assessment.

The invention discloses a catalyst for adsorption-photo-thermal synergistic degradation of VOCs and a preparation method thereof.A photocatalytic material TiO2, a thermocatalytic material MnOx and hollow carbon spheres are combined, the hollow carbon spheres (GCS) are used as a supporting framework, MnO2 and TiO2 are introduced into the GCS through a microwave-hydrothermal method, and the catalyst is prepared. The preparation method comprises the following steps: firstly, preparing a TiO2 (at) MnO2 (at) GCS multifunctional photo-thermal catalytic material with a core-shell structure by using an in-situ calcination reduction process, and constructing a photo-thermal synergistic catalytic composite system with adsorption, thermocatalysis and photocatalysis, so that the problems of high energy consumption, low photocatalysis quantum efficiency and the like of traditional thermocatalysis can be solved, and VOCs can be efficiently degraded.

The invention relates to a method for efficiently recovering valuable components of complex low-grade platinum-palladium-oxygen-sulfur mixed ore. The method comprises the following steps: (1) pretreating the low-grade platinum-palladium-oxygen-sulfur mixed ore to be treated; (2) carrying out primary enrichment on useful components; (3) secondary enrichment by a pyrogenic process; (4) performing different-structure ore making to obtain different-structure sulfonium ore; (5) separating different ores to obtain alloyed gold, silver-containing copper sulfide concentrate and nickel sulfide cobalt concentrate; (6) separating alloyed gold; and (7) respectively refining by-products copper and nickel. According to the method, the efficient recovery effect on the valuable components of the complex low-grade platinum-palladium-oxygen-sulfur mixed ore is remarkable, the recovery rates of the valuable components such as platinum, palladium, gold, silver, copper and nickel are all 60% or above, the recovery benefits are good, the process is stable, adaptability is high, and the method has important significance on comprehensive utilization of low-grade platinum-palladium ore resources poor in copper and nickel.

The invention discloses a method, a primer group and a kit for sequencing an adenovirus whole genome, and belongs to the technical field of DNA (Deoxyribose Nucleic Acid) detection. The method for sequencing the whole genome of the adenovirus comprises the following steps: S1, carrying out a first round of amplification by adopting a first round of amplification primers with sequences as shown in SEQ ID NO.1-SEQ ID NO.93 in a table 1; and S2, carrying out a second round of amplification by adopting the second round of amplification primers with the sequences as shown in SEQ ID NO.94-SEQ ID NO.285 in the table 2. The average length of data sequenced by the method is 0.8 kb, compared with the short read length of a second-generation platform, the uniformity of adenovirus coverage can be greatly improved, meanwhile, the adenovirus type coverage is comprehensive, and the method can be widely popularized.

The invention relates to the technical field of detection, in particular to a method for detecting PTH1-84 by liquid chromatography-mass spectrometry. According to the method, an object to be detected is extracted through antibody immunocapture, PTH1-84 protein is effectively enriched, and after elution, UPLC-MS detection is carried out, so that a good detection effect is achieved.