54results about How to "Efficient enrichment" patented technology

A method for preparing a capacitive deionization anode for phosphate adsorption and desorption, and its application, belong to the field of inorganic phosphorus wastewater adsorption and enrichment. This invention aims to solve the problems of existing adsorption materials, such as low phosphate adsorption capacity, difficulty in reusing the adsorbent, or poor conductivity. Method: 1. Preparation of a PEDOT / YMOF-NH2 composite material; 2. Preparation of a capacitive deionization anode. Application: It is used for the adsorption and desorption of phosphates in phosphate-containing water. This invention is used for the preparation of a capacitive deionization anode for phosphate adsorption and desorption.

The invention discloses a xanthene nitroreductase-responsive fluorescent dye compound as well as a preparation method and application thereof, and belongs to the technical field of xanthene derivatives. The fluorescent dye compound can be used for quickly and specifically recognizing overexpressed nitroreductase in tumor cells; after the fluorescent probe acts with nitroreductase, the fluorescence intensity of the fluorescent probe at 718 nm is obviously enhanced, and the fluorescent probe has excellent targeting capability on mitochondria; meanwhile, under the excitation of illumination with the specific wavelength of 660 nm, an acting product of the fluorescent dye compound and nitroreductase can efficiently generate reactive oxygen species (ROS), and then the effect of specifically killing tumor cells is achieved. The xanthene nitroreductase response type fluorescent dye compound provided by the invention can achieve the goal of integration of diagnosis and treatment of solid tumors, and has important clinical application value.

The invention belongs to the technical field of environmental pollution treatment and water treatment, and particularly relates to a method for degrading organic pollutants by coupling a nano confinement catalytic membrane with micro-nano bubbles. The method comprises the following steps: adding micro-nano bubble water and an oxidizing agent into an organic pollutant solution, regulating the pH value to prepare a pretreatment mixed solution, and enabling the pretreatment mixed solution to pass through a nano confinement catalytic membrane to catalytically degrade the organic pollutants, the nano confinement catalytic membrane comprises an organic polymer membrane substrate and a catalyst loaded in the organic polymer membrane substrate; the loading capacity of the catalyst is 5 to 30 mg. The problems that antibiotics are difficult to degrade, pollutant concentration is easily caused by membrane separation, a catalyst is difficult to recycle, and membrane flux and catalytic activity are difficult to balance are solved, the method has the advantages of being high in degradation efficiency, stable in membrane flux, easy to recycle the catalyst and wide in application range, and wastewater containing organic pollutants can be efficiently treated.

The invention relates to the technical field of wastewater treatment, in particular to an electrocatalytic organic wastewater treatment device which comprises a tank body, an outer barrel and an inner barrel, the outer barrel and the inner barrel are arranged in the tank body, the inner barrel is arranged in the outer barrel, a first reaction area is arranged in the inner barrel, a second reaction area is formed between the inner barrel and the outer barrel, and a third reaction area is formed between the outer barrel and the tank body. A communicating hole is formed in the inner barrel and is communicated with the first reaction area and the second reaction area, and an overflow hole is formed in the outer barrel; a feeding assembly is arranged in the first reaction area, polytetrafluoroethylene balls are arranged in the second reaction area, and a waste treatment assembly is arranged in the third reaction area. Meanwhile, non-free-radical active species such as singlet oxygen generated by air on the surfaces of an electric field, ultrasonic waves and a polytetrafluoroethylene material are combined to form a double-effect oxidation system under the combined action of free radicals and non-free radicals, so that the degradation efficiency of organic pollutants is remarkably improved, and the application range of the organic pollutants is remarkably widened.

The invention relates to a tumor-related DNA methylation marker combination, which is selected from at least one of the following: a target sequence as shown in SEQ ID NO: 1-SEQ ID NO: 10, a nucleic acid fragment with the length of at least 15 oligonucleotides or a complementary nucleic acid fragment thereof, and the nucleic acid fragment comprises at least one methylation site indicated by CG. The invention also relates to a detection kit for the methylation marker combination. According to the invention, the combination of the co-methylation states of a plurality of specific methylation regions is used for distinguishing and analyzing the occurrence of tumors, especially kidney cancer, the specific methylation combination has high sensitivity for distinguishing the occurrence of kidney cancer, and the detection method is simple, convenient and feasible. The primer pair combination of the kit overcomes the defect of false positive caused by detection mismatch of a single methylation site in the aspect of primer sequence design, and the interaction between a plurality of methylation biomarker primers and probe pair combinations is considered.

The invention discloses Fe (at) MoS2 hydrogel for sensitizing immunotherapy and a preparation method of the Fe (at) MoS2 hydrogel. The hydrogel comprises ferrous sulfate, molybdenum disulfide and a hyaluronic acid hydrogel matrix. The hydrogel can continuously release iron ions in a tumor slightly acidic environment, Fe is reduced into Fe by utilizing the catalytic action of MoS, and circular supply of Fe is realized, so that tumor cells are efficiently and enduringly induced to generate ferroptosis. More importantly, it is found that the Fe-coated MoShydrogel can specifically up-regulate expression of IL-36 gamma and CXCL2 in a tumor microenvironment for the first time, and by activating an IL36 gamma / CXCL2 / CXCR2 signal axis, mononuclear macrophages are recruited and remodeled, and the immunosuppression microenvironment is reversed. In-vivo experiments show that when the hydrogel is combined with an immune checkpoint inhibitor (such as an alphaPD-1 antibody), a remarkable synergistic anti-tumor effect can be generated, and the complete remission rate is greatly improved.

The present application relates to a method for detecting a site edited by a base editor on a nucleic acid, and a kit for carrying out the method. The present application also relates to a method for detecting editing efficiency or off-target effect of a base editor editing a nucleic acid.

The present application relates to waste metal material recycling technical field, disclose a kind of from multiple metal elements selectively adsorbed method of gold ion enrichment, including using pyridine triazine derivative as ligand and loaded in macroporous silicon-based material as adsorption material, from the acid aqueous solution containing gold ion and other metal ions and enrichment gold ion;The pyridine triazine derivative structure is as follows, wherein, R is C3-C5 straight-chain or branched alkyl.The present application adopts adsorption material, and Au (III) has high efficient selective enrichment effect, it is applicable to from the weak acid and strong acid gold-containing waste liquid containing multiple other metal elements adsorption and recovery precious metal gold element.Adsorption material itself has the characteristics of strong acid, strong alkali hydrolysis, simple preparation process, low cost, more conducive to in industrial production from gold-containing waste liquid efficiently recover precious metal gold element.

The invention provides a recarburization and acid reduction method for strengthening formation of brick red soil mineral binding state organic carbon. The recarburization and acid reduction method comprises the following steps: S1, constructing a functional microbial population; S2, activating and modifying minerals; S3, preparing a multi-layer gradient coating composite preparation; according to the invention, a synergistic construction method of'functional strain selection + specific phage regulation 'is adopted for the first time, and the selected strains are all strains with strong adaptability to the laterite acidic habitat, and can simultaneously realize multiple functions of microbial residual carbon formation, extracellular polymeric colloid synthesis, iron reduction coprecipitation and the like. According to the method, multiple targets of efficient enrichment of mineral binding state organic carbon, especially short-sequence mineral binding state organic carbon, remarkable improvement of soil pH, great increase of corn yield and the like are achieved at the same time, comprehensive benefits are remarkable, and the method is environmentally friendly and high in sustainability.

The invention provides a quaternary high-entropy alloy nano enzyme as well as a preparation method and application thereof, and belongs to the technical field of nano biological materials and biological medicines. The quaternary high-entropy alloy nano-enzyme comprises a single-phase alloy and polyvinylpyrrolidone, the single-phase alloy is composed of four metal elements of M, rhodium, iridium and ruthenium, the surface of the single-phase alloy is modified with the polyvinylpyrrolidone, the element M is selected from palladium or platinum, and the particle size of the quaternary high-entropy alloy nano-enzyme is smaller than 10 nm. The quaternary high-entropy alloy nano-enzyme disclosed by the invention can penetrate through a blood brain barrier, is enriched at a cerebral hemorrhage focus, and comprehensively blocks reactive oxygen species (ROS) cascade reaction after cerebral hemorrhage.

This invention provides a method for the synergistic separation of rubidium and cesium and the removal of silicon and aluminum from lithium slag. The method comprises the following steps: mixing lithium slag with an organic acid and performing a leaching reaction; after solid-liquid separation, obtaining a leachate and a leaching residue; applying an external field to the leachate to induce silicic acid condensation in the leachate to form silica gel; after solid-liquid separation, obtaining a desiliconized leachate and silica gel; mixing the desiliconized leachate with a complex-breaking agent and performing a complex-breaking treatment; then adding a precipitant to the solution after the complex-breaking treatment to precipitate aluminum ions; after solid-liquid separation, obtaining a rubidium-cesium leachate and an aluminum-containing precipitate; mixing the rubidium-cesium leachate with an extractant and performing rubidium-cesium extraction to separate a rubidium-cesium-rich organic phase and an aqueous phase. Furthermore, the method of this invention is simple, the reaction conditions are mild, it conforms to the concept of green chemical development, and it has good prospects for industrial application.

The application discloses a dopamine magnetic molecular imprinting polymer and a preparation method and application thereof. The molecular imprinting polymer is prepared by using a surface imprinting method; in the preparation, a virtual template 3-hydroxy-4-methoxyphenethylamine is preassembled with a functional monomer in a solution, then a magnetic carrier Fe3O4@SiO2-MPS, a crosslinking agent and an initiator are added, and the carrier surface is reacted under inert gas protection to obtain a polymerization product, and the polymer is obtained after elution of the template molecule. The virtual template strategy is used to reduce the preparation cost, and the template leakage in application is avoided, so that the problem of false positive results is avoided; the imprinting factor of the polymer for dopamine can reach 7.25, the selectivity is excellent, and the stability is good; the polymer can be used as a solid-phase extraction adsorbent, and is used for efficient separation, enrichment and detection of dopamine in biological samples (especially urine), and has a good application prospect in the field of biomarker detection related to disease diagnosis.

The invention provides a potato-tower-shaped nanofiber as well as a preparation method and application thereof, and belongs to the technical field of solid-phase microextraction. The preparation method of the potato-tower-shaped nanofiber comprises the following steps: mixing and dissolving a fiber-forming polymer, an amino precursor, a spinning aid and a solvent I to prepare an electrostatic spinning solution; preparing nanofibers by using an electrostatic spinning technology; the preparation method comprises the following steps: soaking nanofibers in water, and washing and drying after soaking; dissolving an aldehyde group precursor and a template agent into a solvent II to obtain a mixed solution, dipping the nanofiber into the mixed solution, adding a catalyst, and then performing solvothermal reaction to obtain the potato-tower-shaped nanofiber. A large number of uniformly-distributed and orderly-arranged sheet structures are inlaid on the surface of the nanofiber, and the whole fiber is in a potato tower shape; the potato-tower-shaped nanofiber has excellent extraction performance on a micro-plastic loaded plasticizer. The potato-tower-shaped nanofiber disclosed by the invention shows good application potential in the fields of sample pretreatment and solid-phase microextraction.

This application provides a method for enriching lithium from low-grade lithium clay ore. The method includes the following steps: obtaining low-grade lithium clay ore; crushing and roasting the low-grade lithium clay ore to obtain sinter; scrubbing the sinter to cause it to rub against itself; performing secondary screening on the scrubbed sinter to obtain a primary screening material with a particle size less than or equal to 0.83 mm and a secondary screening material with a particle size less than or equal to 0.015 mm; and performing flotation on the primary screening material to obtain flotated material, wherein the flotated material is combined with the secondary screening material to form lithium concentrate. This method for enriching lithium from low-grade lithium clay ore can efficiently develop and utilize lithium resources in low-grade lithium clay ore.

The invention relates to a method for separating myocardial cell-derived small extracellular vesicles from in-vitro plasma in vitro and application of the myocardial cell-derived small extracellular vesicles. The method comprises the following steps: (1) providing a ligand capable of being specifically combined with a raney base receptor 2 protein RYR2; (2) separating total small cell extracellular vesicles from the in-vitro plasma sample; (3) co-incubating the ligand and total small extracellular vesicles, so that the ligand is specifically combined with the small extracellular vesicles which are derived from myocardial cells and express RYR2 on the surface, and a ligand-vesicle compound is formed; (4) combining the ligand-vesicle compound with a solid-phase carrier so as to separate the myocardial cell-derived extracellular vesicles of which the surfaces express RYR2 from the mixture; and (5) washing and / or eluting the ligand-vesicle compound combined on the solid-phase carrier to obtain enriched small extracellular vesicles derived from myocardial cells.

The application discloses a device and a process for reinforcing degradation of agricultural waste based on intermittent aeration coupling carbon quantum dots, and belongs to the technical field of anaerobic fermentation. The device mainly comprises a feeding pool, a gas collecting device, a heat preservation layer, an anaerobic reactor, an intermittent aeration device and a discharge pool. The treatment process is as follows: livestock and poultry manure is put into the feeding pool, inoculated sludge and carbon quantum dots are added, and after being uniformly stirred, the mixture is pumped into the anaerobic reactor, intermittent aeration is carried out in the anaerobic reactor through the aeration device, the temperature in the anaerobic reactor is controlled to be 30-37 DEG C, the pH is controlled to be 7-8, and the hydraulic retention time is controlled to be 25-35 days; an exhaust valve is opened, and biogas generated is collected; a discharge valve is opened, and a certain volume of fermentation products is discharged into the discharge pool. Micro-aeration is used to provide growth of facultative bacteria and to produce more cellulase and protease hydrolysis enzymes, so that higher hydrolysis efficiency is achieved. The carbon quantum dots are used to enrich methanogenic electroactive bacteria, reinforce degradation of cow manure and improve methanogenic efficiency.

The invention discloses a catalytic diesel oil decoloring and purifying method and application thereof, and belongs to the technical field of petrochemical industry. The method comprises the following steps: carrying out extractive distillation on catalytic diesel oil, and cutting out narrow fractions; carrying out hydrogenation reaction on the narrow fraction in a micro-channel reactor filled with a Ni-Mo-W / TiO2-SiO2 nickel catalyst to obtain a hydrogenation product; and carrying out gas-liquid separation on the hydrogenation product, and carrying out aromatic hydrocarbon enrichment on the separated liquid-phase refined oil through an activated clay pre-adsorption bed and a silica gel-alumina composite adsorbent main adsorption bed in sequence. Through the three links of raw material pretreatment, hydrogenation decolorization and aromatic hydrocarbon enrichment, deep decolorization is realized under the ultra-low pressure condition of 0.5 MPa-1. 0 MPa, meanwhile, the retention rate of aromatic hydrocarbons exceeds 97%, and the distillation range of the product can be accurately controlled. The color number of the obtained product is smaller than 10, the aromatic hydrocarbon content is larger than 95%, and the standard requirement of the SA-1000 high-boiling-point aromatic hydrocarbon solvent is met.

The application discloses a kind of near-infrared long afterglow luminescent nano probes, its preparation method and application, belong to near-infrared long afterglow material field. With mesoporous silica as template, with zinc acetate, gallium nitrate, tin chloride, chromium acetate, chromium nitrate and yttrium nitrate as doped metal raw materials, long afterglow luminescent core is obtained by reaction and calcination;The outer layer of long afterglow luminescent core is coated with manganese dioxide, to obtain pre-product;Pre-product is surface modified, and coupling target molecule plerixafor, i.e. near-infrared long afterglow luminescent nano probe. The near-infrared long afterglow luminescent nano probe has good biological safety, can be effectively enriched in the tumor area in vivo after mouse tail vein injection, can realize high-sensitivity imaging of multiple small tumors in vivo under red light LED irradiation, and the imaging time is short, suitable for intraoperative imaging, can be used for the imaging of breast cancer tumor model to guide tumor resection.

The application belongs to the technical field of biology and relates to an anti-OX40L nanobody and application thereof. The anti-OX40L nanobody comprises a heavy chain variable region, the amino acid sequence of which is shown as SEQ ID NO:1, and the amino acid sequences of the complementarity determining regions CDR1, CDR2 and CDR3 thereof are shown as SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5 respectively. The application obtains the nanobody targeting OX40L from a llama nanobody natural library through phage display technology, does not need animal immunization, and the obtained antibody can specifically recognize Human OX40L recombinant protein, HEK293 hOX40L overexpression cells and CHO-K1 cynoOX40L overexpression cells, and exhibits good species cross-reactivity and binding activity. Based on the blocking potential of the nanobody on the OX40 / OX40L signal pathway, the nanobody shows a broad prospect in treating inflammatory and autoimmune diseases.

The invention discloses a full-automatic online extraction-post-column derivatization combined device for aldehydes and application of the full-automatic online extraction-post-column derivatization combined device. The device integrates on-line in-tube solid-phase microextraction, liquid chromatographic separation, post-column derivatization reaction and mass spectrometry detection modules, and realizes on-line rapid reaction of chromatographic eluent and a derivatization reagent by using a three-way mixer. The method is mainly used for full-automatic accurate analysis of endogenous aldehyde metabolic markers such as hexanal and heptanal in body fluid such as human urine and plasma, biological matrix interference is effectively removed through on-line enrichment, and the detection sensitivity is remarkably improved; meanwhile, the device can be extensively applied to rapid screening and quantification of trace aldehydes in complex samples such as traditional Chinese medicines, foods, environments and plants. The method provided by the invention realizes automation of the whole process from sample pretreatment to detection, solves the problems of tedious and time-consuming traditional off-line derivation operation and poor reproducibility, and has the advantages of high flux, low cost, no pollution to mass spectrum and the like.

The invention relates to the technical field of natural product enrichment, in particular to a method for enriching 4-(alpha-L-rhamnosioxy)-benzyl glucoside and related metabolites thereof in moringa oleifera bud seedlings, which comprises the following steps: 1) removing the skin of moringa oleifera seeds to obtain peeled moringa oleifera seeds; 2) treating the peeled moringa seeds with aromatic amino acid; and (3) carrying out germination culture on the treated peeled moringa oleifera seeds. According to the method provided by the invention, efficient enrichment of 4-(alpha-L-rhamnosioxy)-benzyl glucoside and related metabolites thereof in the moringa oleifera bud seedlings is realized by directionally adjusting the metabolic flux, the method meets the requirements of safety and process stability of the food industry, the cost is controllable, and the method is suitable for industrial production. And a brand new technological approach is provided for subsequent development of moringa oleifera functional food.

This invention discloses a turtle peptide with angiotensin-converting enzyme (ACE) inhibitory activity, its preparation, and its application, belonging to the field of biotechnology. Using turtle as raw material, this invention obtains peptide components with a molecular weight less than 5 kDa through enzymatic hydrolysis and ultrafiltration. Sequencing reveals three peptide sequences with ACE inhibitory activity: AAGPAGGNLN, EAGAQGPPGA, and GARGDK. In vitro activity experiments verified the ACE inhibitory function of the three turtle peptides. The turtle peptide combination prepared by this invention exhibits significant ACE inhibitory activity and can be used to develop functional foods or antihypertensive drugs. Furthermore, the preparation method is environmentally friendly, efficient, and suitable for industrial production.

This invention discloses a lithium-sulfur battery binder, its preparation method, and its application, belonging to the field of lithium-ion battery technology. The binder is prepared by grafting polyvinyl alcohol (PVA) with 3-(3,4-dihydroxyphenyl)-DL-alanine (DOPA) onto the PVA molecular chain, with a molar ratio of (0.9~1.2):(0.2~1). This invention, through directional molecular structure design of the binder, simultaneously achieves rapid lithium-ion conduction, improved electrochemical reaction kinetics, and optimized electrode mechanical structure stability, solving the industry problem of traditional lithium-sulfur battery binders' single-performance limitations and inability to meet multiple electrode requirements. The preparation method employs a controllable grafting reaction to functionalize the binder's polymer backbone, introducing sufficient polar functional groups and lithium-ion conduction active sites onto the molecular chain. This balances the stability of the polymer chain's cross-linking structure with electrode interface affinity, synergistically improving interfacial ion transport efficiency. The binder provided by this invention has broad application prospects in lithium-sulfur batteries.

The present application provides a kind of fine particle uranium mineral nelson centrifugal reselection feasibility determination method and separation method.Three nelson centrifugal ore dressing is carried out to fine particle uranium ore sample, to determine whether the fine particle uranium mineral is suitable for using nelson centrifugal ore dressing.If it is determined that nelson centrifugal concentrator is an effective separation device, then the method of nelson centrifugal ore dressing-table concentrator-reselection tailings flotation is used to obtain uranium concentrate.The feasibility determination method provided by the present application is simple and easy to operate.The present application combines nelson centrifugal ore dressing, table concentrator and flotation ore dressing method, combines the density, particle size and surface characteristics of mineral, obtains uranium concentrate with higher U grade, improves the U recovery rate of uranium concentrate, provides a new method for effective separation of fine particle uranium mineral, and provides guidance for existing fine particle uranium mineral ore dressing process.

The utility model belongs to wastewater biological treatment technical field discloses a kind of zeolite fluidized bed intermittent nitrosation reactor, including water inlet system, aeration system, stirring device, screen gauze isolator, overflow weir, water outlet system and reactor body;The water inlet system is connected with the bottom of reactor body, the aeration system is connected with the bottom of reactor body, the stirring device extends vertically along reactor body longitudinal center line, paddle is located in reactor middle work area, the screen gauze isolator is straddled in the opening at the upper end of the reactor body, the overflow weir is arranged in the upper portion of the reactor body, the water outlet system is connected with the overflow weir, the reactor body is filled with zeolite filler.The utility model utilizes natural zeolite to adsorb and enrich free ammonia, realizes the efficient enrichment of low concentration free ammonia, and the trans-arched gauze isolator isolates zeolite biofilm in reaction area, realizes the separation of flocculent sludge, and improves effluent quality.

This invention discloses a microfluidic detection device based on quantum dot luminescence, comprising a microfluidic chip, including a microfluidic chip body, a sample liquid inlet, a sample chamber, a main channel, at least one branch channel, and at least one detection unit. The detection unit includes a magnetic bead chamber, a mixing channel, a first inverted U-shaped channel, a detection chamber, and a second inverted U-shaped channel arranged sequentially. An electromagnet is located on the back of the detection chamber, which is connected to a three-electrode system. A reagent chamber is located above the detection chamber, and a paraffin valve is located between the reagent chamber and the detection chamber. A heating element is located on the back of the paraffin valve. An electrochemical workstation is also included. At least one detection component includes a rotating filter and a photon counting detector, with the rotating filter facing the front of the detection chamber and the photon counting detector facing the rotating filter. This invention also discloses a microfluidic detection method. The microfluidic chip of this invention is driven entirely by gravity, eliminating the need for an external pump; it can perform simultaneous quantitative detection of multiple viruses.

The invention provides a method for recovering noble metal ruthenium in a Ru / MgO waste catalyst, and belongs to the technical field of noble metal recovery. The method comprises the following steps: S1, carrying out mixed reaction on the Ru / MgO waste catalyst and a saturated ammonium acetate solution, and then carrying out solid-liquid separation to obtain ruthenium-rich filter residues; s2, mixing the ruthenium-rich filter residues with a sodium hypochlorite solution with the pH value of 6.5-7.5 for reaction, and filtering to obtain filtrate containing RuO4 < 2->; s3, adsorbing the filtrate by using strongly basic anion resin, and eluting to obtain an eluent; adding formic acid into the eluent to obtain a precipitation product; and reducing the precipitation product to obtain the noble metal ruthenium. The carrier preferential dissolution and targeted oxidation strategy is adopted, strong acid corrosion and highly toxic RuO4 generation are avoided, raw materials are cheap, equipment is conventional, waste liquid can be recycled, the final ruthenium recovery rate is larger than or equal to 99.5%, and the purity is larger than or equal to 99.9%.

This invention belongs to the field of materials science, specifically disclosing an electrocatalyst, its preparation method, and its application. The electrocatalyst of this invention comprises a copper layer, a copper nanosheet array, and a quaternary ammonium salt polymer; the copper nanosheet array is disposed on the surface of the copper layer; the copper nanosheet array is composed of arranged copper nanosheets; the quaternary ammonium salt polymer is distributed on the surface of the copper nanosheets and between adjacent copper nanosheets. The electrocatalyst of this invention uses a quaternary ammonium salt polymer supported on the surface of and between the copper nanosheets, enabling the electrocatalyst to possess a robust and efficient gas-liquid-solid three-phase interface in a strongly acidic medium, effectively improving the selectivity, carbon conversion rate, and operational lifespan of the electrocatalyst for CO2 reduction.

The application discloses a kind of mixed salt resource utilization preparation agricultural potassium sulfate and industrial grade sodium chloride, it is related to solid waste comprehensive utilization technical field, the method is removed by the step-by-step precipitation of magnesium, calcium of sodium carbonate by removing magnesium of organic matter oxidation degradation organic matter in mixed salt, impurity, then refined by ion flotation, subsequently obtain sodium chloride and potassium sulfate product by gradient crystallization in turn, finally remaining mother liquor backflow reuse, precipitate waste residue pyrolysis preparation biochar, form closed loop cycle;The application realizes the efficient degradation of organic pollutants in high-organic industrial mixed salt and the deep removal of impurities, effectively improves the refining purity of salt solution and the raw material adaptation range;At the same time, gradient crystallization is used to separate salt and closed loop cycle in whole process, directional separation is used to prepare industrial grade sodium chloride and agricultural potassium sulfate, and the mother liquor and waste residue are all converted into available resources, forming a mixed salt resource utilization system with high value utilization and harmless disposal synergy.