18results about How to "Efficient enrichment" patented technology

The invention belongs to the technical field of environmental pollution treatment and water treatment, and particularly relates to a method for degrading organic pollutants by coupling a nano confinement catalytic membrane with micro-nano bubbles. The method comprises the following steps: adding micro-nano bubble water and an oxidizing agent into an organic pollutant solution, regulating the pH value to prepare a pretreatment mixed solution, and enabling the pretreatment mixed solution to pass through a nano confinement catalytic membrane to catalytically degrade the organic pollutants, the nano confinement catalytic membrane comprises an organic polymer membrane substrate and a catalyst loaded in the organic polymer membrane substrate; the loading capacity of the catalyst is 5 to 30 mg. The problems that antibiotics are difficult to degrade, pollutant concentration is easily caused by membrane separation, a catalyst is difficult to recycle, and membrane flux and catalytic activity are difficult to balance are solved, the method has the advantages of being high in degradation efficiency, stable in membrane flux, easy to recycle the catalyst and wide in application range, and wastewater containing organic pollutants can be efficiently treated.

The application discloses a kind of near-infrared long afterglow luminescent nano probes, its preparation method and application, belong to near-infrared long afterglow material field. With mesoporous silica as template, with zinc acetate, gallium nitrate, tin chloride, chromium acetate, chromium nitrate and yttrium nitrate as doped metal raw materials, long afterglow luminescent core is obtained by reaction and calcination;The outer layer of long afterglow luminescent core is coated with manganese dioxide, to obtain pre-product;Pre-product is surface modified, and coupling target molecule plerixafor, i.e. near-infrared long afterglow luminescent nano probe. The near-infrared long afterglow luminescent nano probe has good biological safety, can be effectively enriched in the tumor area in vivo after mouse tail vein injection, can realize high-sensitivity imaging of multiple small tumors in vivo under red light LED irradiation, and the imaging time is short, suitable for intraoperative imaging, can be used for the imaging of breast cancer tumor model to guide tumor resection.

The application belongs to the technical field of biology and relates to an anti-OX40L nanobody and application thereof. The anti-OX40L nanobody comprises a heavy chain variable region, the amino acid sequence of which is shown as SEQ ID NO:1, and the amino acid sequences of the complementarity determining regions CDR1, CDR2 and CDR3 thereof are shown as SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5 respectively. The application obtains the nanobody targeting OX40L from a llama nanobody natural library through phage display technology, does not need animal immunization, and the obtained antibody can specifically recognize Human OX40L recombinant protein, HEK293 hOX40L overexpression cells and CHO-K1 cynoOX40L overexpression cells, and exhibits good species cross-reactivity and binding activity. Based on the blocking potential of the nanobody on the OX40 / OX40L signal pathway, the nanobody shows a broad prospect in treating inflammatory and autoimmune diseases.

This invention discloses a turtle peptide with angiotensin-converting enzyme (ACE) inhibitory activity, its preparation, and its application, belonging to the field of biotechnology. Using turtle as raw material, this invention obtains peptide components with a molecular weight less than 5 kDa through enzymatic hydrolysis and ultrafiltration. Sequencing reveals three peptide sequences with ACE inhibitory activity: AAGPAGGNLN, EAGAQGPPGA, and GARGDK. In vitro activity experiments verified the ACE inhibitory function of the three turtle peptides. The turtle peptide combination prepared by this invention exhibits significant ACE inhibitory activity and can be used to develop functional foods or antihypertensive drugs. Furthermore, the preparation method is environmentally friendly, efficient, and suitable for industrial production.

The present application provides a kind of fine particle uranium mineral nelson centrifugal reselection feasibility determination method and separation method.Three nelson centrifugal ore dressing is carried out to fine particle uranium ore sample, to determine whether the fine particle uranium mineral is suitable for using nelson centrifugal ore dressing.If it is determined that nelson centrifugal concentrator is an effective separation device, then the method of nelson centrifugal ore dressing-table concentrator-reselection tailings flotation is used to obtain uranium concentrate.The feasibility determination method provided by the present application is simple and easy to operate.The present application combines nelson centrifugal ore dressing, table concentrator and flotation ore dressing method, combines the density, particle size and surface characteristics of mineral, obtains uranium concentrate with higher U grade, improves the U recovery rate of uranium concentrate, provides a new method for effective separation of fine particle uranium mineral, and provides guidance for existing fine particle uranium mineral ore dressing process.

The utility model belongs to wastewater biological treatment technical field discloses a kind of zeolite fluidized bed intermittent nitrosation reactor, including water inlet system, aeration system, stirring device, screen gauze isolator, overflow weir, water outlet system and reactor body;The water inlet system is connected with the bottom of reactor body, the aeration system is connected with the bottom of reactor body, the stirring device extends vertically along reactor body longitudinal center line, paddle is located in reactor middle work area, the screen gauze isolator is straddled in the opening at the upper end of the reactor body, the overflow weir is arranged in the upper portion of the reactor body, the water outlet system is connected with the overflow weir, the reactor body is filled with zeolite filler.The utility model utilizes natural zeolite to adsorb and enrich free ammonia, realizes the efficient enrichment of low concentration free ammonia, and the trans-arched gauze isolator isolates zeolite biofilm in reaction area, realizes the separation of flocculent sludge, and improves effluent quality.

This invention belongs to the field of materials science, specifically disclosing an electrocatalyst, its preparation method, and its application. The electrocatalyst of this invention comprises a copper layer, a copper nanosheet array, and a quaternary ammonium salt polymer; the copper nanosheet array is disposed on the surface of the copper layer; the copper nanosheet array is composed of arranged copper nanosheets; the quaternary ammonium salt polymer is distributed on the surface of the copper nanosheets and between adjacent copper nanosheets. The electrocatalyst of this invention uses a quaternary ammonium salt polymer supported on the surface of and between the copper nanosheets, enabling the electrocatalyst to possess a robust and efficient gas-liquid-solid three-phase interface in a strongly acidic medium, effectively improving the selectivity, carbon conversion rate, and operational lifespan of the electrocatalyst for CO2 reduction.

The application discloses a kind of mixed salt resource utilization preparation agricultural potassium sulfate and industrial grade sodium chloride, it is related to solid waste comprehensive utilization technical field, the method is removed by the step-by-step precipitation of magnesium, calcium of sodium carbonate by removing magnesium of organic matter oxidation degradation organic matter in mixed salt, impurity, then refined by ion flotation, subsequently obtain sodium chloride and potassium sulfate product by gradient crystallization in turn, finally remaining mother liquor backflow reuse, precipitate waste residue pyrolysis preparation biochar, form closed loop cycle;The application realizes the efficient degradation of organic pollutants in high-organic industrial mixed salt and the deep removal of impurities, effectively improves the refining purity of salt solution and the raw material adaptation range;At the same time, gradient crystallization is used to separate salt and closed loop cycle in whole process, directional separation is used to prepare industrial grade sodium chloride and agricultural potassium sulfate, and the mother liquor and waste residue are all converted into available resources, forming a mixed salt resource utilization system with high value utilization and harmless disposal synergy.

This invention relates to a method for recovering associated zinc from copper concentrate during copper smelting, belonging to the field of non-ferrous metal smelting and mineral processing technology. The method includes: preparing and grinding copper concentrate to obtain a finely ground slurry; the -0.074 mm particles in the finely ground slurry constitute 92-95% of the total solid particles; mixing the finely ground slurry with a copper collector and a zinc inhibitor; the zinc inhibitor contains sulfur dioxide, and the mixing temperature of the zinc inhibitor and the finely ground slurry is 40-50°C; and using microbubble flotation technology to separate copper from zinc concentrate using flotation, obtaining copper concentrate and zinc concentrate; the foam diameter of the microbubble flotation is 30-80 μm. This invention achieves efficient inhibition of zinc minerals by controlling the copper flotation separation temperature, at which waste sulfur dioxide is used synergistically as a zinc inhibitor, further controlling the particle size of the fine grinding and the foam diameter during flotation, thus constructing an integrated copper-zinc separation and zinc recovery process.

The application discloses a cultivation method of strontium-rich cordyceps militaris, and relates to the technical field of microbial cultivation, and comprises the following steps: step S1, preparation of a culture medium; step S2, activation of a strain; step S3, liquid seed liquid cultivation; step S4, solid-state cultivation; and step S5, harvesting and drying. The strontium-rich cordyceps militaris cultivated by the cultivation method can realize efficient enrichment of strontium elements, and can guarantee the biomass of the cordyceps militaris and the content of core active ingredients.

This invention discloses an exosome subpopulation enrichment device and method based on dielectrophoresis, belonging to the fields of molecular biology and clinical testing technology. The device includes a pretreatment tube, a dielectrophoresis separation tube, and a collection chamber. The inner wall of the pretreatment tube is modified with a pH-responsive polyelectrolyte brush, a pre-filter membrane is disposed at the bottom, and a non-contact microelectrode array is disposed on the upper part of the outer wall. The dielectrophoresis separation tube is sleeved outside the pretreatment tube, and its inner wall is covered with a conductive layer as a counter electrode. An annular fluid channel is formed between the two tubes, and the area where the microelectrode array is located in the annular fluid channel constitutes the dielectrophoresis enrichment zone. A horizontal microchannel is formed on the side wall of the dielectrophoresis separation tube, and the inlet end of the horizontal microchannel is connected to the top of the dielectrophoresis enrichment zone. The collection chamber is connected to the outlet end of the horizontal microchannel. This invention overcomes the limitations of traditional particle size sieving, enabling precise enrichment and extraction of exosome subpopulations from different cell sources or functional states under shear-free conditions, while maintaining the complete biological activity of the exosomes.

This application discloses a composite material, its preparation method, and its application, relating to the field of materials technology. The composite material, by mass, comprises 55-75 parts of a matrix resin and 15-25 parts of functional materials. The functional materials include a porous adsorbent, a metal oxide, and a negative ion release agent. The porous adsorbent in the functional materials, due to its large specific surface area, can effectively physically adsorb and enrich organic pollutants such as formaldehyde, catalytically oxidizing them into harmless substances. Negative oxygen ions can charge oxygen molecules in the air, promoting the neutralization, aggregation, and sedimentation of pollutant molecules, or decomposing them. Based on the synergistic effect of the components of the functional materials, the composite material provided in this application can effectively purify air under light-free conditions.

The application discloses an inhalable lung-targeting hybrid vesicle biomimetic nanomaterial and a preparation method and application thereof, and belongs to the technical field of biological medicines. 3+ / Sr 2+ The AuSr-GA network obtains the hybrid vesicle biomimetic nanomaterial (AuSr-GA@BMEVs) with synergistic antibacterial, anti-inflammatory and blood vessel repair promoting functions, can systemically cope with drug-resistant bacterial infection, excessive inflammation and tissue damage. The material is suitable for atomization inhalation, can be efficiently delivered to the lung in a targeted manner, has good biological safety, and provides an integrated new strategy with high efficiency and low toxicity for the treatment of pneumonia.

This invention relates to the field of nanobody technology, specifically to anti-CLDN6 nanobodies and their applications. This invention provides a class of anti-CLDN6 nanobodies with high affinity and specificity. These nanobodies can specifically recognize and bind to CLDN6 targets in various solid tumors, including ovarian cancer, liver cancer, endometrial cancer, testicular cancer, and gastric cancer. The nanobodies of this invention can serve as core recognition elements for constructing various therapeutic and diagnostic platforms, such as chimeric antigen receptor T cells, NK cells, bispecific antibodies, antibody-drug conjugates, or molecular imaging probes. This technical solution addresses the technical problem of the lack of highly specific anti-CLDN6 nanobodies in existing technologies, providing an effective means for the immunotherapy of CLDN6-positive solid tumors.

This invention belongs to the field of cosmetic raw material preparation technology, specifically relating to a method for decolorizing Xinjiang erythromycin, its products, and applications. The method includes: raw material pretreatment: pulverizing Xinjiang erythromycin and processing it using high-pressure instantaneous explosion technology; extraction: extracting the pretreated material with an ethanol-water solution, and obtaining a crude extract of Xinjiang erythromycin after solid-liquid separation; activated carbon decolorization: adding ethanol to the crude extract and mixing evenly, then adding activated carbon for decolorization treatment, followed by solid-liquid separation after decolorization; resin purification: loading the supernatant obtained after decolorization onto a resin column, performing gradient elution, collecting the ethanol eluent rich in phenolic acids, concentrating, and drying. This invention achieves an excellent balance between efficient decolorization and retention of highly active ingredients. It can strongly adsorb and remove dark pigments while significantly reducing the adsorption loss of the target phenolic acids.

The application discloses a multi-channel enzyme typing foot fungus reversible detection patch, and belongs to the technical field of biological detection and shoe functional material. The patch comprises a breathable and dye-resistant surface layer, a multi-channel enzyme sensing middle layer and a low-sensitivity back adhesive bottom layer. The sensing middle layer is a heterogeneous four-layer composite structure formed by a phase separation process, comprising a polyurethane continuous phase layer and a superabsorbent resin dispersed phase layer, which respectively load a ternary acid-base indicator and a fungus cell wall specific degradation enzyme. The application releases acidic substances by enzymatic degradation of the fungus cell wall, realizes multi-color typing by using the difference in metabolic acidity of different fungi, and restores the color after removing the detection environment, thereby realizing reusability. The application effectively eliminates the interference of sweat, bacteria and the like, solves the false positive / false negative problem of the existing humidity color-changing insole, has the advantages of high specificity, high accuracy and non-onetime use, and can be widely applied to the shoe material integration field.