89 results about "Strong binding" patented technology

The invention discloses tetrapeptide KA4 with an anti-fatigue function as well as a preparation method and application of the tetrapeptide KA4, and belongs to the technical field of biology. The tetrapeptide KA4 is screened from pacific salmon protamine enzymatic hydrolysate, the amino acid sequence is KYPA, and the tetrapeptide KA4 has strong binding capacity with KEAP1, AMPK and LDH, can significantly reduce the serum lactic acid content of a fatigue model mouse and significantly increase the load swimming time of the fatigue model mouse, has an anti-fatigue function, and can be applied to development of functional food for relieving fatigue.

The invention provides a GPRC5D single-domain antibody B11, and the GPRC5D single-domain antibody B11 has relatively strong binding activity and reaction specificity on GPRC5D. The invention provides an amino acid sequence of a single-domain antibody, an antibody derivative, a biological material and application thereof. The single-domain antibody provided by the invention can be used for constructing a chimeric antigen receptor T cell, and the chimeric antigen receptor T cell has stronger killing power on a target cell.

The invention is suitable for the technical field of biological medicine and nuclear medicine, and provides a PD-L1 targeting compound, a radioactive tracer agent and a preparation method and application of the PD-L1 targeting compound and the radioactive tracer agent. The radioactive tracer provided by the invention has strong binding force with PD-L1, can accurately locate PD-L1 in vivo, has excellent in-vivo targeting performance, and can achieve the purpose of tumor molecular imaging through nuclear medicine imaging; meanwhile, noninvasive visualization of PD-L1 expression is achieved, non-invasive tumor diagnosis can be effectively completed through small animal PET / CT verification, and the clinical application prospect is wide; in addition, the PD-L1 targeting compound and the radioactive tracer derived from the PD-L1 targeting compound further have the advantages of being simple in preparation process, low in cost, high in specificity, good in in-vivo and in-vitro stability, long in imaging period, easy to clinically convert and the like, and the comprehensive performance is outstanding.

The application provides a preparation method of a functionalized phage probe and application of the functionalized phage probe in detection of escherichia coli. The functionalized phage is a recombinant phage (M13KO7@SaBP) in which streptavidin binding peptide (SaBP) is fused and expressed at the N terminal of the main capsid protein P8 protein of M13K07 phage. In the application, the gene encoding SaBP (MDVEAWLGAR) is inserted into the N terminal of the P8 protein gene of M13K07 phage by site-directed mutagenesis to prepare M13K07@SaBP. The stable signal amplification is realized by using the abundant P8 protein (about 2700) of M13 phage and the super strong binding force of biotin-streptavidin, the interference of food sample matrix is reduced by combining the magnetic separation technology, and a detection method of escherichia coli with high sensitivity and small sample matrix interference is constructed.

The invention provides a novel anti-IL-17A single-domain antibody as well as preparation and application thereof, and belongs to the technical field of cellular immunology. The amino acid sequences of the antibody provided by the invention comprise: (1) the amino acid sequences of HCDR1, HCDR2 and HCDR3 respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; and / or; and (2) an amino acid sequence having at least 80% of sequence consistency with the amino acid sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The antibody has the activity or performance of strong binding capacity and stability, good blocking effect and the like. The invention also provides application of the antibody in preparation of drugs for treating autoimmune diseases.

The present invention belongs to the technical fields of molecular biology and immunology, and provides a camelid-derived nanobody that specifically binds to the trypsin analogue TrypLE and its application. It has a heavy chain variable region VHH, and the VHH contains the following CDRs: CDR1 with the amino acid sequence shown in SEQ ID NO:1, CDR2 with the amino acid sequence shown in SEQ ID NO:2, CDR3 with the amino acid sequence shown in SEQ ID NO:3. The VHH includes 4 framework regions of FR1-4, and FR1, FR2, FR3 and FR4 are arranged alternately with CDR1, CDR2 and CDR3 in sequence. It is a nanobody with high neutralizing activity, has a strong binding ability to TrypLE protein, can effectively recognize TrypLE. This nanobody has a small molecular weight, low immunogenicity, better solubility and stability, and a longer CDR region, providing potential application value for the clinical detection of TrypLE residue content.

The invention discloses a hybridoma cell strain secreting an anti-CD4 antibody, the antibody and application of the hybridoma cell strain in detection of CD4 of cattle and sheep, and belongs to the technical field of biology. The hybridoma cell strain is 3F9 and is preserved in the China Center for Type Culture Collection on June 13, 2025, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2025183. The anti-CD4 antibody 3F9 secreted by the hybridoma cell strain provided by the invention has the advantages of high titer and strong binding affinity with natural antigens, and can be used for detecting natural cattle CD4 and sheep CD4 molecules.

This invention belongs to the field of antigen recognition technology, specifically relating to an antibody or its antigen-binding fragment that targets and recognizes mCD155, and its applications. The antibody targeting and recognizing mCD155 includes sequences as shown in SEQ ID NO:8 and SEQ ID NO:16 or as shown in SEQ ID NO:24 and SEQ ID NO:32; it has a strong binding affinity to the mCD155-his protein; and CAR-T cells constructed based on this antibody exhibit excellent killing (apoptotic) activity against mCD155-mediated tumors.

Provided in the present invention are a solid form of a cyclic amine derivative, a preparation method therefor, and a pharmaceutical composition, specifically relating to a solid form of a compound of formula I or a salt thereof, a preparation method therefor, and a pharmaceutical composition. The compound of formula I or the salt thereof provided by the present invention has strong binding ability to apo(a) and high stability, and can be better used in clinical practice.

The utility model discloses a multi-layer graphene heat-conducting sheet with strong binding force and rebound resilience, which comprises a plurality of graphene films which are punched by a laser and irradiated by an ultraviolet lamp for 1-2 minutes; coating the surface of the first graphene film with a first silica gel layer; placing a second graphene film on the first silica gel layer; enabling a part of the first silica gel layer to permeate into the holes of the first graphene film and the second graphene film; repeating the stacking to form a transverse graphene film and silica gel composite material block; cutting along the direction of the stacking thickness to form a plurality of stacked slices; the upper end face and the lower end face are subjected to degumming operation, graphene fiber filling operation is carried out after a hollow part is formed, all the graphene films can be connected with one another, and the multi-layer graphene heat-conducting sheet is high in binding force and rebound resilience. Therefore, the graphene has good bonding performance, can bear high compression rate without cracking, and fundamentally solves the cracking problem of the conventional graphene heat-conducting sheet.

The present invention provides a starch-fatty alcohol complex and a preparation method thereof. The method comprises: subjecting starch and a fatty alcohol having a cycloalkane group to ultrahigh pressure treatment. Under ultrahigh pressure treatment, the starch and the cycloalkane group-containing fatty alcohol exhibit strong binding, effectively improving binding rate, crystallinity, and thermal stability. The resulting starch-fatty alcohol complex has a stable structure and high application value.

This invention belongs to the field of biotechnology, providing a mouse anti-human ELL2 monoclonal antibody, its preparation method, and its applications. This invention successfully obtained a 2E1 anti-human ELL2 monoclonal antibody and established a hybridoma cell line capable of stably producing this antibody. The binding affinity (KD) of the obtained 2E1 monoclonal antibody to human ELL2 protein reached the picomolar level (approximately 10^-3 kDa). ‑12 The antibody (M) exhibits extremely strong binding ability. Cross-reactivity tests confirmed that it binds only to human ELL2, and shows no binding signal with highly homologous human ELL1, ELL3, and mouse ELL2, thus completely solving the specificity problem of existing technologies.

A metal-chelating resin includes (a) a compound represented by Formula (I):or a stereoisomeric form thereof or a salt thereof, wherein R1, R2, R3, R4, Ra, Rb, Rc and Rd are as defined herein; and (b) an organic polymer resin having at least one complementary reactive functional group covalently linked with at least one linking group of the compound represented by Formula (I).

A metal-chelating resin includes (a) a compound represented by Formula (I):or a stereoisomeric form thereof or a salt thereof, wherein R1, R2, R3, R4, Ra, Rb, Rc and Rd are as defined herein; and (b) an organic polymer resin having at least one complementary reactive functional group covalently linked with at least one linking group of the compound represented by Formula (I).

The application discloses a single-domain antibody against CD99 protein and a coding gene and application thereof. The single-domain antibody Nab99-30 against CD99 protein comprises three complementarity determining regions CDR1, CDR2 and CDR3, characterized in that the amino acid sequences are sequentially shown in SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3; the amino acid sequence of the single-domain antibody Nab99-30 is shown in SEQ ID NO. 4, and the nucleotide sequence of the coding gene is shown in SEQ ID NO. 5. The single-domain antibody against CD99 protein screened by the application has the advantages of small volume, good stability, high activity and the like compared to common monoclonal antibodies, has strong binding capacity with CD99 protein, has high specificity and high affinity, and can be applied to preparation of a reagent for detecting CD99 protein or treatment of tumors with extracellular abnormal expression of CD99.

The present invention discloses a coating with high infrared emissivity and strong bonding force on the surface of a carbon / carbon composite material, its preparation method and application, which relates to the technical field of infrared radiation coating materials. The coating components and the mass fractions of each component are as follows: 22-26 parts of carbon nanotubes, 6-8 parts of carbon nanotube dispersant, 15-20 parts of nano carbon black, 3-5 parts of phenolic resin, 8-11 parts of urea, and 5000-6100 parts of isopropanol. By applying carbon nanotubes and using the foaming effect of urea for pore regulation, the present invention is beneficial to reducing the areal density of the coating, and better weight reduction benefits can be obtained. Moreover, raw materials with low prices are selected as the main materials of the coating, the composition of the coating is optimized, and an infrared high-emissivity coating with good interfacial bonding with the carbon / carbon composite material and excellent thermal shock resistance is prepared through a simple preparation process.

The application discloses an egg yolk antibody against Nocardiosis and a preparation method and application thereof, and relates to the technical field of biological medicines. A light chain sequence of the egg yolk antibody comprises a sequence shown in SEQ ID NO. 1, and a heavy chain sequence comprises a sequence shown in SEQ ID NO. 2. The application expands culture of pathogenic bacteria Nocardia, prepares an inactivated bacterin vaccine, and immunizes hens, so that the hens produce egg yolk antibodies against Nocardia through an immune response. After immunization, the application collects eggs, extracts the egg yolk antibodies against Nocardiosis from egg yolk by using a water dilution method. It is verified that the egg yolk antibodies have a strong binding capacity with Nocardia, can effectively inhibit Nocardia, and the titer can reach 1:64000 at the highest.

The application relates to a G-quadruplex RNA fluorescent ligand and a preparation method and application thereof, a structure formula of the G-quadruplex RNA fluorescent ligand is shown in the following formula: the compound can be specifically targeted and can be subjected to fluorescent imaging in a solution system and a cell system, a high-throughput fluorescent screening method is established in the solution and the cell system, and an organic small-molecule compound with strong binding capacity to G-quadruplex RNA can be effectively identified and screened from a small-molecule drug library; the screening result of the method is high in accuracy, the affinity and biological activity of a candidate compound can be quickly determined by evaluating the change of a fluorescent signal, and therefore the research and development process of an antitumor drug is accelerated; and the application belongs to the field of biological medicines.

The embodiment of the invention discloses a vehicle control method and device, equipment and a storage medium, and belongs to the technical field of vehicle control. The method comprises the steps that a first vehicle path is obtained with a current path point of a first vehicle as a starting point; determining a first blocked vehicle set of the first vehicle based on the first vehicle path in combination with a waypoint binding relationship; the waypoint binding relation comprises a strong binding relation and a weak binding relation, the strong binding relation is used for indicating the relation between waypoints contained in the area where the vehicles are not allowed to be parallel, and the weak binding relation is used for indicating the relation between waypoints contained in the area where the at least two vehicles are allowed to be parallel; and sending first movement information to the first vehicle under the condition of determining that the first vehicle can reach the first waypoint based on the first blocked vehicle set. According to the method, the path planning pressure for the first vehicle is reduced, the blocking problem is predicted in advance, existence of the problem is predicted before the problem is generated, the blocking problem can be solved in time subsequently, and the smoothness of the driving process of the first vehicle is improved.

The invention discloses a nano antibody targeting to Nectin-4 and an application of the nano antibody. The nano antibody comprises a heavy chain variable region, the heavy chain variable region comprises CDR1, CDR2 and CDR3, the amino acid sequence of the CDR1 is SIAMA, the amino acid sequence of the CDR2 is SISGGGSTNYADSVKG, and the amino acid sequence of the CDR3 is DLDYGLGSGEENDY. The Nectin-4-targeted nano antibody disclosed by the invention has relatively strong binding force with a Nectin-4 antigen, and has the advantages of strong tumor targeting property, fast tumor enrichment and long retention.

The invention discloses a decentralized identity strong binding and security recovery method. The method comprises the following steps: S1, deploying an intelligent contract on a block chain network; s2, a user generates a main public and private key pair through a smart contract, the smart contract allocates a unique decentralized identifier to a main public key, sets the main public key to be in an unverified state, and stores an association relationship between the decentralized identifier and the main public key; and S3, for the decentralized identifier in the unverified state, the user submits a binding assertion to the smart contract for validity verification, and after verification is passed, the decentralized identifier is converted into a verified state and granted with authority. The invention also discloses a decentralized identity strong binding and security recovery system. According to the method, the intelligent contract can realize operation of corresponding functions, intervention of a centralized mechanism is not needed in the whole process, the autonomous controllable characteristic of decentralized identity is reserved, and unification of safety, credibility and usability is realized through multi-party authoritative endorsement and a strict verification mechanism.

The application discloses a static self-assembly preparation method of a high-nickel ternary material nano-barium titanate core-shell structure. The technology realizes uniform coating of nano-particles on the surface of a base through electrostatic attraction by means of surface charge regulation and layer-by-layer self-assembly mechanism, that is, by immersing positively charged high-nickel ternary material into negatively charged nano-barium titanate slurry liquid. The application can obtain core-shell materials with strong binding force and controllable structure. The process is simple in operation and low in energy consumption. The barium titanate coating layer based on the coordination of the physical barrier and electric field modulation dual mechanisms effectively improves the interface stability and the electrochemical kinetic performance.

The invention belongs to the field of biology, and discloses an anti-chicken IFN-gamma protein detection antibody, which contains a heavy chain variable region and a light chain variable region, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is shown as SEQ ID No.3; the amino acid sequence of the light chain variable region of the monoclonal antibody is as shown in SEQ ID No.4. The detection antibody has relatively strong binding capacity to chicken IFN-gamma with different concentrations under the same antibody capturing condition, and particularly has excellent suitability when being matched with a specific coated antibody for use. Meanwhile, the invention also provides a group of antibody pairs for detecting chicken interferon or duck interferon and application of the antibody and the antibody pair for detecting the IFN-gamma.

The present invention relates to the fields of medicinal chemistry, radiopharmaceutical chemistry, and clinical nuclear medicine, and in particular to a compound targeting CDK4 / 6 and a radioactive tracer derived therefrom. The CDK4 / 6-targeting compound has the structures shown in the following formulas (I) and (II). The radioactive tracer provided by the present invention has strong binding affinity with CDK4 / 6, can accurately locate CDK4 / 6 in vivo, exhibits excellent in vivo targeting performance, and achieves tumor molecular imaging through nuclear medicine imaging. Formula (I); Formula (II).

The invention belongs to the technical field of nano antibodies, and provides a nano antibody OVA-3-23 for resisting chicken ovalbumin and application of the nano antibody OVA-3-23. The nano antibody OVA-3-23 for resisting the chicken ovalbumin has an amino acid sequence as shown in SEQ ID NO. 1. According to the prepared nano antibody OVA-3-23 for resisting the chicken ovalbumin, in the field of directional fixation, accurate anchoring of the chicken ovalbumin is achieved with high specificity, and the defects of impurity interference and fuzzy positioning in traditional fixation are overcome; in the field of immunodetection, high specificity and strong binding force guarantee the detection accuracy, colloidal gold, enzyme and other multi-component components are matched, and the chicken ovalbumin is efficiently identified from food on-site fast screening to laboratory fine detection; in the field of enrichment and purification, a high-purity product is efficiently separated from a complex sample. Especially, reliable support is provided for application scenes such as food detection, feed quality monitoring, immunology and vaccine development, and an efficient solution is provided for detection, research and quality control related to chicken ovalbumin.

The invention discloses a method for rapidly cleaning and transferring a wafer-level two-dimensional material without damage. According to the method, a covalent bond is formed and a stable and regular self-assembled monomolecular layer is constructed by utilizing a strong binding force between a modifier molecule and a growth substrate, and the monomolecular layer effectively reduces the surface energy of the growth substrate, so that the interaction force between the growth substrate and a two-dimensional material is remarkably weakened, and the performance of the two-dimensional material is improved. The material is more easily stripped from the growth substrate; secondly, according to the method, low-viscosity PDMS is selected as a transfer medium, the medium can provide proper adhesive force, and it is ensured that the two-dimensional material cannot fall off due to insufficient adhesive force in the transfer process and cannot be damaged or residues due to too high adhesive force; and after the transfer is completed, the low-viscosity PDMS is more easily stripped from the target substrate, so that residues, bubbles and defects are reduced, and the original electrical and optical characteristics of the two-dimensional material can be effectively maintained.

The invention discloses a preparation method of high-binding-force conductive microspheres for ACF (anisotropic conductive film), and belongs to the technical field of conductive materials. The method comprises the following steps: introducing a bifunctional sulfydryl functional reagent with sulfydryl and double bonds in a polymerization reaction process, and firmly combining the sulfydryl to the surface of a polymer microsphere; carrying out surface activation treatment by utilizing the strong affinity of sulfydryl to palladium ions; and forming a metal coating on the surface of the microsphere through chemical plating. Sulfydryl is stabilized through double-bond copolymerization, palladium ion adsorption and coating binding force are enhanced through the sulfydryl, and the binding strength of the conductive microsphere and a connecting substrate, the adhesive force of a chemical coating and a microsphere matrix and the conductive performance and long-term reliability of ACF are remarkably improved. The conductive microsphere prepared by the invention has the advantages of simple process, controllable cost, excellent performance and the like, is suitable for high-density and high-reliability electronic interconnection, and can be applied to the fields of preparation of anisotropic conductive adhesive films and packaging of related electronic products.

A metal-chelating resin includes (a) a compound represented by Formula (I):or a stereoisomeric form thereof or a salt thereof, wherein R1, R2, R3, R4, Ra, Rb, Rc and Rd are as defined herein; and (b) an organic polymer resin having at least one complementary reactive functional group covalently linked with at least one linking group of the compound represented by Formula (I).

A use of a compound or a medicinal derivative thereof in inhibiting absent in melanoma 2 (AIM2) protein activity is provided and belongs to the field of protein inhibitory medicines. Compared with traditional broad-spectrum immunomodulators, the compound has a strong targeting effect, and is more accurate, rapid, effective, safe and stable. Moreover, the compound has a strong binding force with human AIM2 protein and mouse AIM2 protein, and binding constants are as high as KD=1.56E−6M and KD=5.12E−6M respectively. Compared with traditional biological inhibitors, the compound has advantages of easy storage, stable activity, small molecular weight, lower production cost and easy absorption.

The application provides an anti-IGFBP5 nanobody and a preparation method, application and product thereof, and belongs to the technical field of biotechnology.The anti-IGFBP5 nanobody provided by the application contains a CDR region shown in SEQ ID NO:1-3, or one or more amino acid sequences with a sequence similarity of at least 90% with SEQ ID NO.1-3 respectively, the anti-IGFBP5 nanobody prepared by the application has strong binding capacity and can inhibit tumor cell growth.