411 results about "High titer" patented technology

The invention relates to the technical field of biological medicines, and particularly discloses an anti-human CD3 rabbit recombinant monoclonal antibody as well as a preparation method and application thereof. The antibody obtains anti-CD3 immune response by immunizing a rabbit, is obtained by combining single B cell sorting, gene cloning and a mammalian cell expression system, and has high affinity and good specificity. The antibody can effectively recognize CD3 positive T cells in human peripheral blood and can be applied to the fields of flow cytometry, immunohistochemistry, T cell function research and the like. Experimental results show that the antibody has the advantages of high titer, strong fluorescence signal, low background and the like, and is superior to the traditional murine anti-CD3 antibody. Amino acid sequences of light chain and heavy chain variable regions of the antibody are further obtained, and controllability and repeatability of antibody expression are achieved. The antibody has important scientific research value and industrial application prospect, and is suitable for various scenes such as T cell marker detection, immune state evaluation, immunotherapy auxiliary research and the like.

The invention belongs to the technical field of vaccines and biology, and particularly relates to a broad-spectrum influenza A vaccine immunogen composition and application. The invention provides a nucleic acid molecule for coding an immunogenic peptide, wherein the immunogenic peptide comprises: M2 protein extracellular region (M2e) peptide fragments which are connected in series; a hemagglutinin protein stem region extracellular domain (HA2) peptide fragment derived from an H7N9 influenza virus subtype; multimerization of the motif peptide fragment; and optionally, one or more linker peptide fragments independently located between the peptide fragments, wherein the polynucleotide molecule comprises a coding nucleic acid of each peptide fragment. The broad-spectrum influenza A vaccine can induce an organism to generate antigen-specific immune response, a high-titer antibody can be generated 14 days after last inoculation, the broad-spectrum influenza A vaccine can completely protect and resist attack of various subtype influenza A viruses, and the broad-spectrum influenza A vaccine has a wide application prospect.

The invention relates to the technical field of biology, and aims to provide a monoclonal antibody 7A12 for identifying gene type 2 swine fever as well as a preparation method and an application method of the monoclonal antibody 7A12. The monoclonal antibody comprises Ig structural domains VH CDR1, VH CDR2 and VH CDR3 of a heavy chain of the antibody, and Ig structural domains VL CDR1, VL CDR2 and VL CDR3 of a light chain of the antibody, wherein the amino acid sequences of the VH CDR1, the VH CDR2 and the VH CDR3 are respectively as shown in SEQ ID NO: 1 to SEQ ID NO: 3; the amino acid sequences of the VL CDR1, the VL CDR2 and the VL CDR3 are respectively as shown in SEQ ID NO: 4 to SEQ ID NO: 6. The antibody provided by the invention has the advantages of high titer, strong specificity, high affinity and the like; the kit has the advantages of high sensitivity and strong specificity, has no cross reaction with the type 1 swine fever vaccine strain, and can identify and diagnose swine fever gene type 2 wild strain infection and vaccine immunity in serology; the method can be further used for establishing a blocking ELISA kit for identifying the gene type 2 hog cholera, and can effectively diagnose and purify the gene type 2 hog cholera virus.

The invention relates to the technical field of biological medicines, in particular to a rabies virus G protein mutant as well as a preparation method and application thereof. The rabies virus G protein mutant is obtained by carrying out point mutation on an extracellular domain of G protein, the mutant realizes soluble expression of the extracellular domain of the G protein, can induce generation of a high-titer neutralizing antibody, and has more excellent cellular immunity and antibody immune response compared with commercial human rabies vaccines and canine rabies vaccines; the inoculation frequency is expected to be reduced, the immune procedure is shortened, and the immune dosage is reduced.

The invention relates to the technical field of molecular biology, in particular to an avian adenovirus serum type 4 colloidal gold test strip based on a monoclonal antibody and application of the test strip. According to the invention, two monoclonal antibodies (6B3 and 8G11) aiming at FAdV4 penton protein are successfully prepared through a hybridoma technology, and both the monoclonal antibodies show high titer and strong serotype specificity. Specific analysis proves that the two monoclonal antibodies can recognize 12 FAdV4 isolates from different sources, and do not have cross reaction with other FAdV serotypes or common poultry pathogens. According to the invention, through systematic optimization of key parameters, the detection sensitivity of the test strip is 7.81 * 10 < 4 > TCID50 / 0.1 mL, and the detection time is only 15 minutes. Clinical sample verification shows that compared with an ELISA method, the total coincidence rate is 98.6%.

The invention discloses an anti-dsDNA antibody or an antigen binding fragment thereof, a detection reagent and a kit, and relates to the technical field of biological detection. The anti-dsDNA antibody or the antigen binding fragment thereof provided by the invention comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO. 2. The detection reagent provided by the invention comprises the anti-dsDNA antibody or the antigen binding fragment thereof. The anti-dsDNA antibody or the antigen binding fragment thereof provided by the invention solves the problems of traceability of positive blood and large-scale supply. By applying the scheme of the invention, the anti-dsDNA with high titer, high accuracy and good stability or the antigen binding fragment thereof can be prepared, and a raw material source with excellent performance is provided for the preparation of a calibration product / a positive control product / a quality control product of an anti-dsDNA detection kit.

The invention belongs to the technical field of biochemical engineering, and particularly relates to an amitriptyline hapten and a preparation method thereof, an amitriptyline antigen and a preparation method thereof, an antibody, a kit and application. The amitriptyline hapten has a structural formula shown as a formula (I) or a formula (II). Compared with an existing structure, the amitriptyline hapten prepared by the invention has a better immune effect. The obtained antibody is high in titer, strong in specificity and high in affinity, the LOD of amitriptyline by using an Elisa method established by the antibody is 0.013 ng / mL, the IC50 of amitriptyline is 0.865 ng / mL, the quantitative detection range is 0.07-470.3 ng / mL, the detection sensitivity is high, and the linear range is wide.

The invention relates to the technical field of immune carrier microspheres, in particular to an immune carrier microsphere constructed by individualized MHC-II combined polypeptide, a vaccine and application of the immune carrier microsphere and the vaccine. A core comprises microspheres constructed by a plurality of MHC-II binding polypeptides, sequences of the MHC-II binding polypeptides are obtained based on HLA gene typing results of an inoculator, and each HLA gene corresponds to at least one high-affinity MHC-II binding polypeptide; a shell is a glucan with sulfydryl or other polymer coating layer, and sulfydryl is used as a coupling group to be connected with an antigen to construct the vaccine. The method has the advantages that competition is avoided, inhibition is generated, a better immune effect is achieved, antibodies of high-titer carrier molecules are not generated, and cross reaction side effects are avoided. The carrier molecular diversity is reduced; side effects are avoided. And antibody immunity and cellular immunity functions are generated. The particle size of the microspheres is controllable, and the immunologic function can be achieved without adjuvants. The preparation process is simple to operate and good in repeatability.

A specific protein fragment capable of representing salmonella as an antigen is obtained through screening and experimental verification, a mouse is immunized with the specific protein fragment as the antigen, and hybridoma cells capable of stably secreting monoclonal antibodies are obtained through a cell fusion technology. And finally, the anti-salmonella monoclonal antibody with high titer and good specificity is prepared. Similarly, the specific protein fragment is used for immunizing a rabbit to obtain the anti-salmonella polyclonal antibody. On the basis of a mouse monoclonal antibody and a rabbit polyclonal antibody, a salmonella colloidal gold immunochromatography detection method is established, the detection method is good in specificity, and a rapid, simple and convenient detection means is provided for detection of salmonella in food.

The invention discloses an mRNA-LNPs Brucella vaccine, an expression vector and a preparation method thereof. The mRNA-LNPs Brucella vaccine comprises at least one of mRNA of an outer membrane protein BP26 and mRNA of an outer membrane protein Omp 25. According to the present invention, the antigen sequence is optimized, the prepared mRNA Brucella vaccine can stimulate the body to produce strong immunogenicity, and compared with the Brucella vaccine prepared before the sequence optimization, the antibody titer is significantly improved. A mouse immune experiment research result shows that high-titer IgG, IgG1 and IgG2a can be generated. Th1 type reaction, Th2 type reaction or mixed Th type reaction is successfully induced.

The present application provides an RNA encoding a varicella-zoster (VZV) glycoprotein E (gE) or a variant thereof, the RNA comprising a nucleotide sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity with the nucleotide sequence represented by any one of SEQ ID NO: 11-18. The eight mRNAs provided by the invention can translate in cells, the expression level of the generated VZV gE protein is high, and the mRNAs can induce a mouse to generate a high-titer gE specific IgG antibody in a manner of intramuscular injection into the body of the mouse through a preparation formed by encapsulating the lipidosome nanoparticles.

The invention discloses chicken infectious anemia virus-like particles as well as a preparation method and application thereof. The invention discloses a virus-like particle vaccine for preventing chicken infectious anemia. The virus-like particle vaccine comprises VP1 and VP2 proteins of chicken infectious anemia viruses. Chicken infectious anemia VP1 and VP2 proteins are expressed by using a baculovirus expression system, immunoblotting shows that the two proteins are successfully expressed in sf9 cells, electron microscope observation finds that the expressed proteins can be autonomously assembled into complete chicken infectious anemia virus-like particles, and the chicken infectious anemia virus-like particles have a space structure similar to that of an original virus and can be used for preparing chicken infectious anemia virus-like particles. Meanwhile, the virus-like particles have the advantages of high titer, high safety, capability of stimulating humoral immunity and cellular immunity and the like. The preparation method disclosed by the invention is simple, can be used for preparing the antigen protein of the chicken infectious anemia virus on a large scale, is high in expression quantity and short in time consumption, greatly reduces the production cost, and is suitable for large-scale production.

The invention discloses prokaryotic expression and purification of Echinococcus multilocularis glutathione S-transferase, and preparation and application of a polyclonal antibody, and relates to the technical field of biology.The technical key point is that the preparation method of the polyclonal antibody of the Echinococcus multilocularis glutathione S-transferase comprises the following steps that S1, RNA in vesicle tissue of Echinococcus multilocularis is extracted, and cDNA is prepared; s2, carrying out PCR (Polymerase Chain Reaction) amplification by taking the cDNA as a template to obtain an EmGST gene which can encode an amino acid sequence as shown in SEQ ID NO: 1; s3, connecting the EmGST gene to a prokaryotic expression vector plasmid through a double digestion enzyme reaction to construct a recombinant vector, then transfecting the recombinant vector to competent escherichia coli, and inducing prokaryotic expression and purification to obtain a recombinant protein; and S4, immunizing a mouse to prepare antiserum by taking the echinococcosis multilocularis glutathione transferase as an antigen, and purifying the antiserum to obtain the polyclonal antibody of the echinococcosis multilocularis glutathione transferase. The ImGST protein polyclonal antibody prepared by the invention has the advantages of high titer and good specificity.

The invention discloses a klebsiella pneumoniae bacteriophage vBKpnPGZMUVR2301 and application thereof, and relates to the technical field of biology. The klebsiella pneumoniae bacteriophage vBKpnPGZMUVR2301 is preserved in Guangdong Microbial Culture Collection Center on October 14, 2024, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 65268-B1. The bacteriophage keeps stable titer in a wide pH range (4-10) and a temperature interval (4-50 DEG C), and the highest titer can reach more than 109 pfu / mL. The bacteriophage can efficiently split klebsiella pneumoniae. The invention provides powerful technical support for developing novel antibacterial drugs and treatment schemes for klebsiella pneumoniae, and shows wide application prospect and potential clinical value.

The invention relates to the technical field of antibodies, in particular to an anti-African swine fever virus p30 protein monoclonal antibody and application thereof, and the anti-African swine fever virus p30 protein monoclonal antibody can be specifically combined with African swine fever virus p30 protein. The anti-ASFV p30 protein monoclonal antibody is prepared by immunizing a BALB / c mouse through an immunological method, and the antibody is high in specificity, titer and affinity and can be applied to immunological detection or antibody detection kits.

The invention provides a monoclonal antibody capable of specifically recognizing a specific metabolic fragment of human amyloid protein and application of the monoclonal antibody, and belongs to the technical field of genetic engineering. The invention provides a hybridoma cell strain AB5H, and the preservation number of the hybridoma cell strain AB5H is CCTCC (China Center For Type Culture Collection) NO: C201255. The hybridoma cell strain AB5H disclosed by the invention can secrete a monoclonal antibody which has relatively high activity and can specifically recognize human beta amyloid protein A beta 40; the monoclonal antibody is high in titer, can be used as a capture antibody, and is used for preparing a human Abeta40 protein quantitative detection kit and quantitatively detecting the content of Abeta40 in cerebrospinal fluid and blood, so that the detection result deviation caused by the fact that the content of Abeta40 in peripheral blood is easily influenced by metabolism, sample treatment conditions and methods and individual differences is overcome, and the detection accuracy is improved. The variation degree of a detection result is reduced, so that a judgment boundary value of a blood A beta detection result is conveniently obtained, and AD early diagnosis is realized.

The invention discloses a hybridoma cell strain secreting an anti-micro-nano plastic monoclonal antibody and application thereof.The hybridoma cell strain comprises a hybridoma cell strain MP-4 and a hybridoma cell strain MP-9, the hybridoma cell strain MP-4 and the hybridoma cell strain MP-9 are obtained through self screening of the inventor, the preservation number of the hybridoma cell strain MP-4 is CCTCC NO: C2024262, the preservation number of the hybridoma cell strain MP-9 is CCTCC NO: C2024262, and the preservation number of the hybridoma cell strain MP-9 is CCTCC NO: C2024262. The preservation number of the hybridoma cell strain MP-9 is CCTCC NO: C2024261. The passage cell strain of the hybridoma cell provided by the invention can stably secrete an anti-broad-spectrum micro-nano plastic protein monoclonal antibody, the secreted antibody has high titer and good sensitivity, can simultaneously recognize PS, PVC, PP, PE and PET, has good broad-spectrum recognizability, provides a core raw material for establishing a broad-spectrum immunodetection method of micro-plastics, and has good application prospects. Wide application prospects are realized.

The invention belongs to the technical field of indication viruses, and particularly relates to a method for improving mouse leukemia virus titer and / or stability. The invention provides an application of mifepristone in improving mouse leukemia virus titer and / or stability. The mifepristone is utilized to integrate the mouse leukemia virus onto the cell genome, so that the amplification of the mouse leukemia virus is completed, the infection efficiency of the mouse leukemia virus is improved, and finally the mouse leukemia virus stock solution with higher titer and better stability is obtained.

The present invention provides a pharmaceutical combination comprising a tetanus-adsorbing vaccine and an antibody against tetanus toxin or an antigen-binding fragment thereof. The pharmaceutical composition is used for preventing and / or treating tetanus. According to the present invention, the combination of the recombinant natural completely humanized monoclonal antibody and the tetanus adsorption vaccine proves that the detection time of the antibody induced by the tetanus adsorption vaccine is advanced, and the high titer level can last for a longer time;

The invention belongs to the technical field of biological medicine, particularly relates to an Alzheimer's disease protein marker, an antibody diagnostic kit and application of the antibody diagnostic kit, and provides the Alzheimer's disease protein marker A beta (1-42). The invention provides a kit for detecting human amyloid A beta based on sandwich ELISA (enzyme-linked immunosorbent assay) of 2B6 and 3A8 antibodies, and further provides a kit for detecting human amyloid A beta based on 2B6 and 3A8 antibodies by predicting B cell epitopes of a template A beta (1-42) protein through ABCpred to reconstruct an immune antigen enhanced Alzheimer disease combined antigen such as SEQ ID NO: 1, called AD-A beta 2 for short, and monoclonal antibodies 2B6 and 3A8 with strong affinity and high titer based on the antigen. The A beta concentration detection line effect of at least 0.5 ng / mL is realized.

The invention belongs to the technical field of immunodetection, and discloses a hapten and an artificial antigen for sulfonamide drug detection, a construction method and an application thereof, the structure of the hapten and the artificial antigen is shown as a formula (1): R1 =-COOH, R2 = R3 = H; r2 =-COOH, R1 = R3 = H; r3 is-COOH, and R1 is equal to R2 and H is equal to H. The invention discloses a novel sulfonamide immune hapten and a construction method of an artificial antigen of the novel sulfonamide immune hapten for the first time, and successfully obtains a specific antibody with high titer, high sensitivity and broad-spectrum recognition capability on the basis of immune animals of the artificial antigen.

The invention discloses an anti-CD3D antibody and application thereof.The antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of CDR-H1 of the heavy chain variable region is shown as SEQ ID NO: 4, the amino acid sequence of CDR-H2 of the heavy chain variable region is shown as SEQ ID NO: 5, and the amino acid sequence of CDR-H3 of the heavy chain variable region is GDL; the amino acid sequence of the CDR-L1 of the light chain variable region is as shown in SEQ ID NO: 6, the amino acid sequence of the CDR-L2 of the light chain variable region is as shown in SEQ ID NO: 7, and the amino acid sequence of the CDR-L3 of the light chain variable region is as shown in SEQ ID NO: 8. The anti-CD3D antibody is a rabbit monoclonal antibody, the obtained antibody is high in titer, clear in specific positioning in immunohistochemical staining and free of non-specific background staining, the antibody is wide in application, and the antibody can be used in pathological immunohistochemical staining experiments and can also be used in Western blot and flow cytometry experiments.

The invention relates to the field of microorganisms, and particularly discloses a highly pathogenic gosling plague virus and a preparation method of an egg yolk antibody of the highly pathogenic gosling plague virus. The invention relates to a gosling plague virus, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), is named as the gosling plague virus RD2311B-E21, is preserved on April 22, 2024, and has the preservation number of CGMCC NO.45964. The gosling plague virus is named as the gosling plague virus RD2311B-E21. The preparation method of the egg yolk antibody comprises the following steps: proliferating and inactivating gosling plague virus RD2311B-E21 to prepare an immunogen; respectively injecting the immunogen into the laying hens for four times to obtain four-immunized eggs; the preparation method comprises the following steps: soaking a tetra-immunized egg in a bromogeramine solution, performing spray fumigation with glutaraldehyde, then separating yolk, treating the yolk with disodium hydrogen phosphate to obtain a turbid solution, and performing acidification extraction, degreasing with octanoic acid, degerming filtration, virus removal by ultrafiltration, ultrafiltration concentration and subpackaging to obtain a finished product. The compound has the advantages of high purity, high titer and good treatment, prevention and control effects.

The invention belongs to the technical field of bacteriophages, and discloses a salmonella bacteriophage with cross-species lysis capability, a bacteriophage composition and application of the salmonella bacteriophage, the salmonella bacteriophage is named as salmonella bacteriophage PC553, the preservation number of the salmonella bacteriophage PC553 is CGMCC No.46166, and the preservation The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 16, 2024, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacteriophage is wide in splitting spectrum and high in titer, shows cross-species strong splitting energy to escherichia coli, can be prepared into drugs, environment disinfectants, feeds, water additives and the like for preventing and treating pigeon salmonellosis and / or colibacillosis for application, effectively prevents and treats spreading of salmonellosis and avoids the problem of secondary infection of escherichia coli, and has a good application prospect in preventing and treating pigeon salmonellosis and / or colibacillosis. The feed additive has the effects of effectively reducing the death and culling rate of pigeons, reducing the positive rate of salmonellosis antibodies, improving the production performance index of pigeons and the like.

The invention discloses a peste des petits ruminants virus specific monoclonal antibody and application thereof.The monoclonal antibody is obtained by screening peste des petits ruminants virus N protein, the amino acid sequence of the peste des petits ruminants virus N protein is shown as SEQ ID NO: 1, the peste des petits ruminants virus N protein is obtained by transcription of a peste des petits ruminants virus N gene, and the peste des petits ruminants virus N gene is shown as SEQ ID NO: 2. The nucleotide sequence of the codon-optimized peste des petits ruminants virus N gene is shown as SEQ ID NO: 2. The monoclonal antibody provided by the invention can be specifically combined with the peste des petits ruminants virus N protein, the titer is high, the affinity is good, meanwhile, the detection kit prepared based on the antibody pair can effectively carry out peste des petits ruminants virus detection, and a technical support can be provided for epidemiological monitoring and diagnosis of peste des petits ruminants virus infection.

Provided are a fully human TSH receptor (TSHR) blocking monoclonal antibody for treating hyperthyroidism, and a preparation method therefor and a use thereof. The method comprises the following steps: using flow cytometry to sort plasma cells and memory single B cells specifically recognizing a TSHR in the peripheral blood of patients having high titers of TSHR blocking antibody (TSH-stimulating blocking antibody, TBAb), performing in vitro cloning of antibody light and heavy chains and performing recombinant expression, and using hTSHR-CHO cells for antibody property screening and validation, to obtain a blocking monoclonal antibody specifically targeting human TSHR. The fully human TSHR blocking monoclonal antibody can specifically bind to the TSHR, has good inhibitory activity in vitro and in vivo experiments, and has broad application prospects in the development of treatment of Graves' diseases.

The invention discloses an allergen specific IgE and IgG antibody composite quality control product or calibration product as well as a preparation method and application thereof, and belongs to the technical field of biological detection. The preparation method comprises the following steps: obtaining a whole-genome sequence comprising a human IgE constant region gene sequence, a human IgG4 constant region gene sequence and a human IgG1 constant region gene sequence, and preparing a fusion protein simultaneously containing a human IgE Fc segment and a human IgG Fc segment; immunizing a healthy animal by using the allergen to obtain a purified antibody; and carrying out chemical coupling on the purified antibody and the fusion protein to obtain allergen specific IgE and IgG antibodies. By constructing allergen specific IgE and IgG antibodies, the kit can be simultaneously applied to detection of allergens IgE and IgG, has the advantages of high titer, high purity, good dilution linearity and the like, and is beneficial to improvement of diagnosis accuracy and treatment effectiveness.

The invention discloses a group of blocking monoclonal antibodies of an SH1 homologous cluster fully human thyrotropin receptor (TSHR) (a TSH receptor, TSHR) as well as a preparation method and application of the blocking monoclonal antibodies of the SH1 homologous cluster fully human thyrotropin receptor (TSHR). The method comprises the following steps: sorting plasma cells and memory single B cells for specifically recognizing TSHR in peripheral blood of a patient with high TSH receptor blocking antibody (TBAb) titer by using flow cytometry, cloning antibody light and heavy chains in vitro and performing recombinant expression, and performing antibody property screening verification by using hTSHR-CHO cells. The blocking monoclonal antibody specifically targeting the human TSHR is obtained. The fully human TSH receptor blocking monoclonal antibody disclosed by the invention can be specifically combined with TSHR, and can effectively block signal transduction after the TSH is combined with the receptor; the synthesis and secretion of thyroid hormone are inhibited; expression and fibrosis of effector cell orbital fibroblasts TSHR for inhibiting thyroid-related eye diseases are remarkably relieved, and wide application prospects are achieved in treatment of Graves' diseases and diseases such as thyroid eye diseases caused by hyperthyroidism.

The invention belongs to the field of biological medicine, and particularly relates to a lysine phenylacetylation modified hapten, an artificial antigen and a preparation method and application of the lysine phenylacetylation modified hapten and the artificial antigen. Researches show that lysine phenylacetylation modification is increased in phenylketonuria, and on the basis, the invention provides the lysine phenylacetylation modified hapten. The lysine phenylacetylation modified hapten is used for further preparing a lysine phenylacetylation modified polyclonal antibody, and the lysine phenylacetylation modified polyclonal antibody has specific recognition and binding capacity on lysine phenylacetylation modification in phenylketonuria patients, and also has relatively high titer and specificity.

The invention relates to the technical field of gene engineering, and particularly discloses application of a gene recombinant of an adenovirus vector and a p53 gene, and application of the recombinant constructed by utilizing the adenovirus vector and the human p35 gene in preparation of drugs for treating proliferative diseases. The recombinant adenovirus is packaged by adopting an AdMax packaging system framework, the E1 / E2-deleted replication-deficient adenovirus is obtained through intracellular homologous recombination, recombinant virus particles with relatively high titer can be obtained, and the recombinant virus particles can have relatively high expression in cells with proliferative diseases, so that the recombinant adenovirus can be used for treating proliferative diseases. By means of the method, in-vivo transfer of the exogenous p53 gene and efficient expression of the exogenous p53 gene in hyperplasia tissue of a patient are achieved, so that the purpose of treatment is achieved, and gene treatment of hyperplasia diseases is achieved.