The invention provides a reagent strip for joint detection of a syphilis specific IgG antibody and a specific total antibody and a preparation method thereof, relating to a reagent for joint detection of a syphilis specific IgG antibody and a specific total antibody. The reagent strip is provided with two reagent strips, wherein one is provided with a vector plate, a sample adding pad, a colloidal gold pad, a nitrocellulose membrane, a syphilis specific IgG antibody detection line, a control line and an absorbent pad; and the other one is provided with a vector plate, a sample adding pad, a colloidal gold pad, a nitrocellulose membrane, a specific total antibody detection line, a control line and an absorbent pad. The preparation method comprises the following steps: preparing recombinant syphilis antigens TPN17 and TPN47, applying samples of nitrocellulose membranes, preparing colloidal gold, labeling TPN17 and TPN47 with colloidal gold and preparing immunochromatography detection strips. The reagent strip can be used for detecting the syphilis specific IgG antibody and the specific total antibody in the specimens of whole blood, blood serum, blood plasma, cerebrospinal fluid and the like. During detection, the specimen amount is small, special instruments are not needed and the results are directly interpreted by the naked eyes. The reagent strip is simple, convenient, fast, accurate and reliable and has strong specificity and high sensitivity.